KR102538067B1 - Reaction cassette for measuring glycated hemoglobin - Google Patents

Abstract

The present invention is a housing in which one upper surface is open, a guide end is disposed inwardly, and a detachable cartridge is inserted and fixed; a first accommodating area accommodating a first reagent inside the housing after the cartridge is inserted into the housing; a second accommodating area physically separated from the first accommodating area and accommodating a second reagent inside the housing; a measurement area in which the blood sample delivered into the housing reacts with the first reagent or the second reagent to measure the total hemoglobin or glycated hemoglobin content of the blood sample; and a blocking member disposed on one side of the first accommodating region or close to the first accommodating region and guiding the flow of the second reagent when the housing rotates on one axis. to provide.

Description

Glycated hemoglobin measurement cassette {REACTION CASSETTE FOR MEASURING GLYCATED HEMOGLOBIN}

The present invention relates to a cassette for measuring glycated hemoglobin. More specifically, the present invention relates to a glycated hemoglobin measurement cassette that prevents a blood sampling unit from being contaminated by a blood sample and thereby reducing the accuracy of the glycated hemoglobin measurement value, thereby providing an accurate glycated hemoglobin measurement result.

Glycated hemoglobin is one of the hemoglobins contained in human red blood cells. When blood sugar (glucose) in the blood rises, some of the glucose in the blood binds to hemoglobin. Hemoglobin combined with glucose is called glycated hemoglobin and is also called hemoglobin A1c (HbA1c).

A conventional cassette for measuring glycated hemoglobin has separate areas for accommodating reaction reagents and washing reagents, and the reagents are sequentially introduced into a blood sampling unit carrying a blood sample to measure the total hemoglobin content in the blood sample. , The washing reagent is designed to wash the blood sample and measure the content of glycated hemoglobin.

At this time, the blood sample is supported on the front end of the blood sampling unit and goes through a process of being drawn into the measurement cassette. Due to the structure of the blood sampling unit, there is a possibility that the blood sample contaminates the outer circumferential surface of the blood sampling unit, and the cleaning reagent remains on the outer circumference of the blood sampling unit. When reacting with a blood sample, the content of the reacting glycated hemoglobin increases, causing a problem in that the accuracy of the glycated hemoglobin measurement result is lowered.

Therefore, it is urgent to develop a glycosylated hemoglobin measuring cassette capable of preventing the reduction in the accuracy of the measured value of glycated hemoglobin due to the delivery of the washing reagent to the blood sample contaminated by the blood sampling unit.

Prior art for the present invention is disclosed in Korean Registration Publication No. 10-0798471.

An object of the present invention is that when the outer circumferential surface of a blood sampling unit that flows into a glycosylated hemoglobin measuring cassette and delivers a blood sample is contaminated by an incorrect blood sample collection method, a washing reagent is delivered to the contaminated part of the blood sampling unit to increase the measured value of glycated hemoglobin. It is an object of the present invention to provide an glycated hemoglobin measuring cassette that exhibits a more accurate glycated hemoglobin measurement value by preventing a decrease in accuracy in advance and allowing only a quantified blood sample carried in a blood sampling unit to react with the washing reagent.

The above and other objects of the present invention can all be achieved by the present invention described below.

1. One aspect of the present invention relates to a glycosylated hemoglobin measurement cassette.

a housing in which one upper side is opened, a guide end is disposed inwardly, and a detachable cartridge is inserted and fixed;

a first accommodating area accommodating a first reagent inside the housing after the cartridge is inserted into the housing;

a second accommodating area physically separated from the first accommodating area and accommodating a second reagent inside the housing;

a measurement area in which the blood sample delivered into the housing reacts with the first reagent or the second reagent to measure the total hemoglobin or glycated hemoglobin content of the blood sample; and

and a blocking member disposed on one side of the first accommodating area or close to the first accommodating area and guiding the flow of the second reagent when the housing rotates on one axis.

2. In the first embodiment, the first accommodating area may provide a space in which the first reagent or the second reagent flows and reacts with the blood sample.

3. In the first embodiment, the blocking member is disposed to be close to one surface of the housing, and divides the first accommodating area so that the second reagent can flow only under one end of the blocking member.

4. In the first embodiment, the cartridge may store the first reagent and the second reagent separately.

5. In the first embodiment, the cartridge is provided with fastening guides on both sides of the blood sampling unit fastened to the guide end;

a first storage area disposed on one side of the blood sampling unit and storing a first reagent; and

It may include a second storage region disposed on one side of the first storage region and storing a second reagent.

6. In the above 5 embodiments, the blood sampling unit

a front end provided with a capillary through which the blood sample is permeated;

an extension portion extending from the front end portion and bent so that an outer periphery of the front end portion approaches the blocking member; and

It may include a; fastening part supporting the extension part, extending in the longitudinal direction, and having fastening guides disposed on both side surfaces.

7. In the sixth embodiment, the front end may be disposed in the first accommodating area according to Equations 1 and 2 below.

[Equation 1]

_D1 > _D2

[Equation 2]

_L1 > _L2

Here, D ₁ is the distance from one surface of the housing to the front end, D ₂ is the distance from one surface of the blocking member to the front end, L ₁ is the distance from one surface of the housing to one end of the blocking member, and L ₂ is the housing It is the distance from one side to the front end.

8. In the first embodiment, the cartridge includes a blood sampling unit capable of injecting a blood sample into the measurement cassette,

The blood sampling unit may include a front end portion discharging the blood sample into the housing;

an extension portion extending from the front end portion and bent so that an outer periphery of the front end portion approaches the blocking member; and

It may include a; fastening part supporting the extension part, extending in the longitudinal direction, and having fastening guides disposed on both sides thereof.

In the present invention, even when the outer circumferential surface of the blood collection unit inserted into the measurement cassette is contaminated due to operator error, poor blood collection process, or incorrect blood collection environment, the washing reagent inserted into the measurement cassette and accommodated is used to drive or clean the measurement cassette. In the process of moving the reagent, it is not transferred to the blood sample contamination site, so the cleaning reagent does not react with the contaminated blood, and only the blood sample of a certain amount carried in the blood collection unit reacts, so that a more accurate glycated hemoglobin measurement value can be displayed.

1 is a perspective view of a glycosylated hemoglobin measurement cassette according to one embodiment of the present invention.
2 is a front cross-sectional view of the measurement cassette according to FIG. 1;
3 is an exploded perspective view of the measuring cassette according to FIG. 1;
4 is a schematic diagram showing a state in which a cartridge is inserted into a glycosylated hemoglobin measuring cassette according to an embodiment of the present invention.
5 is a schematic diagram showing a state in which a second reagent is stored in a second accommodating region in the glycated hemoglobin measurement cassette according to one embodiment of the present invention.
FIG. 6 is a schematic diagram showing a movement path of a second reagent when the glycated hemoglobin measuring cassette rotates clockwise about one axis in FIG. 5 .

Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings. However, the following drawings are only provided to aid understanding of the present invention, and the present invention is not limited by the drawings. In addition, since the shape, size, ratio, angle, number, etc. disclosed in the drawings are exemplary, the present invention is not limited to the illustrated details.

Like reference numbers designate like elements throughout the specification. In addition, in describing the present invention, if it is determined that a detailed description of related known technologies may unnecessarily obscure the subject matter of the present invention, the detailed description will be omitted.

When 'includes', 'has', 'consists of', etc. mentioned in this specification is used, other parts may be added unless 'only' is used. In the case where a component is expressed in the singular, the case including the plural is included unless otherwise explicitly stated.

In interpreting the components, even if there is no separate explicit description, it is interpreted as including the error range.

When the positional relationship of two parts is described by 'over', 'over', 'under', 'beside', etc., unless 'immediately' or 'directly' is used, there is a One or more other parts may be located.

Positional relationships such as 'top', 'top', 'bottom', and 'bottom' are described based on the drawings, and do not represent absolute positional relationships. That is, the positions of 'upper' and 'lower' or 'upper surface' and 'lower surface' may be changed depending on the observation position.

In this specification, "a to b" representing a numerical range is defined as "≥a and ≤b".

1 is a perspective view of a glycosylated hemoglobin measurement cassette according to one embodiment of the present invention, FIG. 2 is a front sectional view of the measurement cassette according to FIG. 1, FIG. 3 is an exploded perspective view of the measurement cassette according to FIG. 1, and FIG. It is a schematic diagram showing a state in which the cartridge is inserted into the glycated hemoglobin measurement cassette according to one embodiment of the present invention.

Hereinafter, a glycosylated hemoglobin measuring cassette according to one embodiment of the present invention will be described with reference to FIGS. 1 to 4.

The glycated hemoglobin measurement cassette 1000 includes a housing 200, a first accommodating area 300, a second accommodating area 400, a measurement area 500, a sample absorbing part 600 and a blocking member 700. do.

The upper side of the housing 200 is opened to form an open surface 210, and a guide end 240 is disposed to the inside so that the detachable cartridge 100 can be inserted and fixed. More specifically, the guide end 240 may protrude or be provided with a groove, and may be provided singly or in plural numbers, but if the structure allows the cartridge 100 to be inserted, the shape and number are not limited. don't

The housing 200 provides a space in which the cartridge 100 is inserted into the open surface 210 and fixed therein.

The cartridge 100 includes a blood sample, a first reagent, and a second reagent, and the first reagent and the second reagent can be stored separately.

More specifically, the cartridge 100 includes a blood collection unit 110 for holding the blood sample, and the blood collection unit 110 carries blood to be measured for glycated hemoglobin and is first accommodated inside the housing 200. It can be delivered to area 300.

In the first accommodating region, the second accommodating region, the reaction region, etc. described in this specification and claims, "area" means a physical space separated temporally or spatially. For example, the first accommodating area, the second accommodating area, and the reaction area may be the same space from different viewpoints or may be different spaces from the same viewpoint. This is because the solution accommodated in the measurement cassette moves as the measurement cassette rotates.

The cartridge 100 may include a blood sampling unit 110, a first storage area 120, and a second storage area 130, but the blood sampling unit 110 is configured separately from the cartridge 100. It could be.

The blood sampling unit 110 may be provided with fastening guides 111a and 111b on both sides and fastened to the guide end 240 .

The fastening guides 111a and 111b are provided so that when the blood sampling unit 110 is inserted into the housing 200, the position and path are limited, and the blood sampling unit 110 moves the guide end 240. It can be slid along and inserted into the housing 200.

Specifically, the fastening guide is provided along the longitudinal direction on both sides of the blood sampling unit 110, and the fastening guide 111b provided on the other side may be higher than the fastening guide 111a on one side.

When the fastening guides 111a and 111b are provided on both sides of the blood sampling unit 110, they are fastened to the guide end 240, so that the cartridge 100 is inserted by sliding, and the fastening guides 111a and 111b When the height of ) is arranged differently on both sides, the first receiving area 300 may be arranged so that the blood sampling unit 110 is spaced apart from the outer wall of the housing 200 at regular intervals.

The first storage area 120 is disposed on one side of the blood sampling unit 110 and stores a first reagent.

The first reagent may be hemolysis and glycated hemoglobin binding material-beads.

Specifically, HbA1c-binding substances capable of specifically binding to HbA1c and hemoglobin containing surfactant include boronic acid (BA), concanavalin A (Lectin), and antibodies. The glycated hemoglobin beads may be at least any one of agarose beads, sepharose beads, latex beads, and glass beads.

The first reagent binds HbA1c-tagged beads with non-glycated hemoglobin by hemolyzing the blood of the blood sample, and when the reactants thereof are measured, the total hemoglobin content can be measured.

The second storage region 130 is disposed on one side of the first storage region 120 and stores a second reagent.

The second reagent may remove non-glycated hemoglobin by washing the mixture of the first reagent and the blood sample.

More specifically, the cartridge 100 includes a first storage region 120 and a second storage region 130 containing the first reagent and the second reagent. The storage areas 130 are separately stored, and the first storage area 120 and the second storage area 130 are sealed with foil covers so that the first reagent and the second reagent can be stored separately.

The cartridge 100 may further include a handle part 140 , and the handle part 140 is disposed above the first storage area 120 and the second storage area 130 .

The handle part 140 provides a space in which the cartridge 100 can be gripped and transported, and the blood collection part 110 in the cartridge 100 is collected while a blood sample is collected and inserted into the housing 200. access of workers can be effectively prevented.

The cartridge 100 may further include reinforcing ribs 150, and the reinforcing ribs 150 are disposed between the first storage area 120 and the second storage area 130 in plurality.

When the reinforcing rib 150 is disposed, it is easy to maintain the shape of the first storage region 120 and the second storage region 130, and the first storage region 120 and the second storage region 120 and the second storage region against external impact. The risk of breakage of 130 can be reduced.

When the cartridge 100 is inserted into the housing 200 along the guide end 240, the first storage area 120 and the second storage area ( 130), the first reagent and the second reagent may be discharged.

The housing 200 may be made of a transparent resin, and may be, for example, one or more of polycarbonate, (meth)acrylic resin, and polyester resin.

When the housing 200 is made of the above type of transparent resin, the strength is increased to reduce the risk of damage due to impact, and the total light transmittance is increased to grasp the flow of the first reagent and the second reagent, and the measurement area In step 500, it is possible to effectively measure the change of the first reagent and the second reagent in response to the blood sample.

More specifically, since the glycated hemoglobin measurement cassette 1000 measures the contents of hemoglobin and glycated hemoglobin by detecting changes in the light signal passing through the measurement area 500, the housing 200 is made of high-strength transparent resin. In this case, the risk of damage during storage and transportation is reduced, and measurement sensitivity can be effectively increased without the need to form a separate observation window for receiving light in the measurement area 500 .

The housing 200 may be formed by combining a lower case 230 and an upper case 220 .

When the housing 200 includes a lower case 230 and an upper case 220 and is formed by being coupled to each other, the manufacturing efficiency of the HbA1c measurement cassette 1000 is increased, and the first accommodating area 300, the second 2 The receiving area 400, the measurement area 500, and the sample absorbing part 600 are arranged inside the lower case 230 to facilitate configuration.

In one specific example, the housing 200 may be disposed so that a plurality of guide ends 240 protrude. Specifically, the plurality of guide ends 240 are respectively disposed on the lower case 230 and the upper case 220 .

When the guide end 240 is disposed, the blood collection unit 110 of the cartridge 100 can be slidably inserted along the guide end 240, and the guide end 240 is inserted into the cartridge 100. By limiting the direction and location, the cartridge 100 can be positioned at an exact location within the housing 200 .

The lower case 230 may include foil tabs 231 disposed in the first accommodating area 300 and the second accommodating area 400 .

When the moving direction and position of the cartridge 100 is limited by the guide end 240, the foil tab 231 is disposed at a position in contact with the foil cover and moves the foil cover according to the movement of the cartridge 100. is pressed, and the foil cover is finally opened so that the first reagent and the second reagent can be discharged.

In one embodiment, the height of the rear end of the foil tab 231 may be increased compared to the front end.

The foil tab 231 is disposed at the moving position of the first storage region 120 and the second storage region 130, and controls the direction in which the first storage region 120 and the second storage region 130 are moved. When the height of the rear end is increased more than the front end toward the front end, the pressing force against the foil cover is gradually increased according to the height of the foil tab 231, so that the foil cover can be effectively opened by pressing the cartridge 100 with a small force. .

Since the foil tabs 231 are provided in plurality and are spaced apart from the outer wall of the housing 200 by the same length, the foil covers of the first storage region 120 and the second storage region 130 are simultaneously opened. The first reagent and the second reagent are discharged together.

A first member 800 may be disposed between the foil tabs 231, and the first member 800 includes the first reagent discharged from the first storage region 120 and the second storage region 130 and The second reagent may be separated and introduced into the first accommodating region 300 and the second accommodating region 400 .

The first member 800 is bent at the center so that both ends are provided toward the first accommodating area 300 and the second accommodating area 400, respectively, to guide the movement path of the first reagent and the second reagent. It may respectively flow into the first accommodating area 300 and the second accommodating area 400 along the first member 800 .

It is also possible to further restrict the flow of the first reagent and the second reagent by further disposing a vertical blocking guide (not shown) at the center of the first member 800 to prevent mixing of the first reagent and the second reagent. .

In one specific example, the upper case 220 may have one end of a pressing piece 221 elastically fixed to the upper case 220 to press one side of the cartridge 100 .

Specifically, when one end of the pressing piece 221 is bent and placed in the upper case 220, the pressing piece 221 is the first storage area 120 and the second storage area 130 of the cartridge 100. ) By pressing one surface, the cartridge 100 may be fixed to the housing 200.

The pressing piece 221 may further include a protruding protrusion at the other end to increase the force pressing the storage areas 120 and 130, but the first storage area 120 and the second storage area 130 It is not limited as long as it is a structure in which one surface is pressed and fixed with a certain force or more.

The first accommodating region 300 is disposed inside the housing 200 and accommodates the first reagent discharged from the first storage region 120 .

The first accommodating area 300 is disposed on one side of the inside of the cartridge 100, and is preferably disposed directly below a point where the first reagent is discharged.

The first accommodating area 300 provides a space in which the blood sample carried in the blood sampling unit 110 of the cartridge 100 flows in and reacts with the first reagent after the first reagent is stored.

Specifically, the first receiving area 300 has one surface provided as an outer wall of the housing 200 and the other surface partitioned by an outer wall of the measurement area 500, so that one end of the blood sampling unit 110 is immersed in the first reagent. Create a space that can be

In one embodiment, the first accommodating area 300 may provide a space in which the first reagent or the second reagent flows and reacts with the blood sample.

Therefore, the first accommodating area 300 is a space where the first reagent reacts with the blood sample carried in the blood collection unit 110 to form a mixture, and then is temporally separated and the second reagent is introduced and reacted. .

The second accommodating region 400 is configured and arranged to be temporally and spatially separated from the first accommodating region 300 and accommodates the second reagent.

The second accommodating area 400 accommodates the second reagent, and it is preferable that the second accommodating area 400 is disposed directly below the point where the second reagent is discharged.

The second reagent may remove non-glycated hemoglobin by washing the mixture of the first reagent and the blood sample.

Specifically, when the housing 200 is rotated at a certain angle with respect to one axis after the blood sample of the blood sampling unit 110 is first reacted, the liquid second reagent is in the second accommodating area 400 according to the inclination. It is transferred to the first receiving area 300, and the second reagent can wash the blood sample so that only glycated hemoglobin remains in the blood sample.

The measuring area 500 is disposed below the second receiving area 400, and the blood sample delivered into the housing 200 reacts with the first reagent or the second reagent to obtain total hemoglobin or glycated hemoglobin in the blood sample. measure the content of

In a specific example, in the measurement area 500, a blood sample reacted with the first reagent in the first accommodating area 300 is introduced, and a light emitting device disposed outside the housing 200 emits light, and the light is When the hemoglobin is reached, the light signal of the light receiver disposed outside the other side of the housing 200 changes according to the specific absorption or reflected light of the hemoglobin, and the total hemoglobin content can be measured by measuring this.

After the total hemoglobin content is measured, the second reagent in the second accommodating area 400 flows into the first accommodating area 300 as the housing 200 rotates in one direction, and the blood sampling part 110 ) is washed, and when the blood sample is rotated in the other direction, the washed blood sample flows into the measuring area 500, and the content of glycated hemoglobin can be measured in the same way as the total hemoglobin measurement method.

At this time, the glycated hemoglobin ratio may be determined according to Equation 3 below.

[Equation 3]

The sample absorption unit 600 is disposed below the measurement area 500 and absorbs the blood sample and the reagent reaction solution after the reaction has been completed.

The sample absorbing unit 600 is disposed below the measuring area 500 to prevent blood samples or reagents from leaking to the outside according to the rotation of the housing 200, and hemoglobin, glycated hemoglobin and glycated hemoglobin within the measuring area It can absorb all of the remaining materials except for the glycated hemoglobin binding material-beads that bind to.

Meanwhile, the blocking member 700 is disposed on one side of the first accommodating area 300 and restricts the flow of the second reagent when the housing 200 rotates on one axis.

Conventionally, the blood collection part is a length member that supports the blood sample at one end and is directly placed in the first receiving area where the reaction with the first reagent is performed. Blood remains on the outer circumferential surface of the sampling unit, and in this case, the second reagent flows into the first receiving area, and even contaminated blood is washed on the outer circumferential surface of the blood sampling unit. As the content is increased, there is a problem in that the accuracy of the glycated hemoglobin measuring device is finally greatly reduced.

The blocking member 700 restricts the passage through which the second reagent moves inside the first accommodating area 300 to effectively prevent the second reagent from washing contaminated blood in the blood sampling unit 110. there is.

In one embodiment, the blocking member 700 is disposed so as to be close to one surface of the housing 200, and divides the first accommodating area 300 so that the second reagent flows only down one end of the blocking member 700. can do.

Specifically, the blocking member 700 is disposed within or close to the first accommodating area 300 to partition a space within the first accommodating area 300, and the blood collection unit 110 is placed in the first accommodating area. When the housing 200 rotates about one axis in a state of being disposed in the 300, the second reagent is prevented from flowing down the ramp and being delivered to the outer circumferential surface of the blood sampling unit 110.

In one specific example, the blood sampling unit 110 may include a front end 112, an extension 113 and a fastening unit 111.

The fastening part 111 may connect the blood collection part 110 and the first storage region 120 and support the extension part 113 at the same time.

The fastening part 111 is provided with fastening guides 111a and 111b on both sides, is inserted into the guide end 240, and slides to transport the cartridge 100 to a predetermined position in the housing 200. .

The extension part 113 extends from the front end part 112, and the outer circumference of the front end part 112 is bent so as to approach the blocking member 700.

The extension part 113 can approach the front end part 112 to the blocking member 700 to restrict the reaction of the contaminated blood remaining in the extension part 113 or the fastening part 111 with the second reagent. there is.

Preferably, the extension part 113 is bent to approach the blocking member 700, but it is disposed on one side of the blocking member 700 so that the front end part 112 is connected to the extension part 113 or the fastening part 111. ), the shape such as length and angle is not limited as long as it is disposed in a position where it does not react with the contaminated blood remaining in the front end 112, and it is also possible to extend straight from the front end 112.

In addition, when the extension part 113 is disposed, the length of the fastening part 111 can be reduced, and in this case, the possibility of blood remaining on the outer circumferential surface of the blood collection part 110 due to the error of the blood collection worker can be greatly reduced. can

The front end 112 may be disposed in the first accommodating area 300 according to Equations 1 and 2 below.

[Equation 1]

_D1 > _D2

[Equation 2]

_L1 > _L2

Here, D ₁ is the distance from one surface of the housing 200 to the front end 112, D ₂ is the distance from one surface of the blocking member 700 to the front end 112, and L ₁ is the distance from the front end 112 to the housing 200. It is the distance from one side to one end of the blocking member 700, and L ₂ is the distance from one side of the housing 200 to the front end 112.

According to Equations 1 and 2, the front end 112 is spaced apart from one side of the housing 200 to secure a sufficient space to react with the second reagent, and at the same time, the distance from the blocking member 700 is the second reagent. Even if it is introduced along this slope, it is effectively prevented from flowing into the extension part 113 and the fastening part 111, so that the accuracy of the glycated hemoglobin measurement value can be dramatically improved.

The front end 112 is provided with a capillary tube 112a through which the blood sample is permeated.

When the capillary tube 112a is provided, the blood sample can be effectively supported on the front end 112 by using the effect of the capillary tube 112a.

The blood sampling unit 110 has the front end 112 separately so that errors in the blood sampling process can be reduced compared to the conventional blood sampling unit 110, and the front end 112 connected to the extension unit 113 Since only the second reagent is supported, the accuracy of the measurement by reacting with the second reagent is further increased.

Hereinafter, a method of using the glycated hemoglobin measurement cassette will be described.

First, a blood sample to be measured for glycated hemoglobin is supported on the front end 112 of the cartridge 100, and the cartridge 100 is inserted into the housing 200.

At this time, the movement and position of the cartridge 100 are fixed by the fastening of the fastening guide of the blood sampling unit 110 and the guide end 240 within the housing 200 as described above.

The cartridge 100 is disposed in the housing 200, the first reagent and the second reagent are discharged from the first storage region 120 and the second storage region 130, and the second reagent is a blocking member. Movement is restricted according to step 700 and stored in the second accommodating area 400, and the first reagent is first a blood sample in the front end 112 of the blood sampling unit 110 located in the first storage area 120. will react with

When the housing 200 is rotated counterclockwise by a certain angle with respect to one axis, the mixture generated by reacting with the first reagent flows into the measurement area 500, and the total hemoglobin content is first measured.

5 is a schematic diagram showing a state in which a second reagent is stored in a second accommodating region in the glycated hemoglobin measuring cassette according to one embodiment of the present invention, and FIG. 6 is a glycated hemoglobin measuring cassette in FIG. 5 based on one axis. It is a schematic diagram showing the movement path of the second reagent when rotating in a clockwise direction.

5 and 6, after measuring the total hemoglobin content, when the housing 200 is horizontally restored and rotated clockwise again, the second reagent 2A forms a flow F along the ramp and flows into the first accommodating area 300. At this time, the blocking member 700 divides the first accommodating area 300 and extends close to the outer wall of the housing 200 to limit the movement of the second reagent 2A.

The second reagent (2A) flows only under the one end of the blocking member (700).

Meanwhile, the front end 112 is close to the blocking member 700 by the extension 113, and the second reagent 2A, the flow of which is restricted by the blocking member 700, is connected to the extension 113 or fastening. It cannot be delivered to the part 111, and the contaminated blood B remaining on the outer circumferential surface of the extension part 113 or the fastening part 111 is not washed by the second reagent, so it is supported on the front end part 112. Only the blood sample to be measured reacts with the second reagent 2A.

The blood sample reacted with the second reagent 2A contains glycated hemoglobin. For example, when the housing 200 rotates counterclockwise, it flows into the measurement area 500 to obtain more accurate glycated hemoglobin. content is measured.

Therefore, in the glycated hemoglobin measurement cassette 1000 according to the present invention, the blocking member 700 is placed in the housing 200 to restrict the movement of the second reagent, thereby reducing the glycated hemoglobin generated when the outer circumferential surface of the blood sampling unit 110 is contaminated. It is possible to prevent an error in the measurement value of the content, and only the front end 112 of the blood collection unit 110 reacts with the second reagent, thereby greatly increasing the accuracy of the HbA1c measurement value. In particular, the front end 112 The overall length of the blood sampling unit 110 provided with the extension unit 113 is reduced, and the front end 112 and the fastening unit 111 are maximally spaced apart by the bent extension unit 113, thereby collecting blood samples. Errors generated in can also be effectively improved.

So far, the present invention has been looked at mainly through embodiments. Those skilled in the art to which the present invention pertains will be able to understand that the present invention may be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments should be considered from an illustrative rather than a limiting point of view. The scope of the present invention is shown in the claims rather than the foregoing description, and all differences within the equivalent scope will be construed as being included in the present invention.

1000: cassette for measuring glycated hemoglobin
100: cartridge 110: blood sampling unit
111: fastening part 111a, 111b: fastening guide
112: front end 112 a: capillary
113: extension 120: first storage area
130: second storage area 140: handle part
150: reinforcing rib 200: housing
210: upper open surface 220: upper case
221: pressing side 230: lower case
231: foil tab 240: guide end
300: first accommodating area 400: second accommodating area
500: measurement area 600: sample absorption unit
700: blocking member 800: first member
2A: second reagent F: second reagent flow
B: contaminated blood

Claims (8)

a housing in which one upper side is opened, a guide end is disposed inwardly, and a detachable cartridge is inserted and fixed;
a first accommodating area accommodating a first reagent inside the housing after the cartridge is inserted into the housing;
a second accommodating area physically separated from the first accommodating area and accommodating a second reagent inside the housing;
a measurement area in which the blood sample delivered into the housing reacts with the first reagent or the second reagent to measure the total hemoglobin or glycated hemoglobin content of the blood sample; and
A blocking member disposed on one side of the first accommodating area or close to the first accommodating area and guiding the flow of the second reagent when the housing is rotated based on one axis;
The cartridge includes a blood sampling unit capable of injecting a blood sample into the measurement cassette,
The blood sampling unit may include a front end portion discharging the blood sample into the housing;
an extension portion extending from the front end portion and bent so that an outer periphery of the front end portion approaches the blocking member; and
A glycosylated hemoglobin measurement cassette comprising: a fastening part supporting the extension part, extending in a longitudinal direction, and having fastening guides disposed on both sides of the fastening part.
The method of claim 1, wherein the first accommodating area
The glycated hemoglobin measurement cassette characterized in that it provides a space in which the first reagent or the second reagent flows and reacts with the blood sample.
The method of claim 1, wherein the blocking member
The glycated hemoglobin measuring cassette characterized in that it is disposed close to one surface of the housing, and divides the first accommodating area so that the second reagent flows only under one end of the blocking member.
The method of claim 1, wherein the cartridge
A glycosylated hemoglobin measurement cassette characterized in that the first reagent and the second reagent are stored separately.
The method of claim 1, wherein the blood sampling unit
Fastening guides are provided on both sides and fastened to the guide ends,
A glycosylated hemoglobin measuring cassette having a first storage region disposed on one side of the blood sampling unit and storing a first reagent and a second storage region disposed on one side of the first storage region and storing a second reagent.
The method of claim 1, wherein the front end
A glycosylated hemoglobin measuring cassette having a capillary through which the blood sample is permeated.
The glycated hemoglobin measuring cassette according to claim 1, wherein the front end is disposed in the first receiving region according to Equations 1 and 2 below:
[Equation 1]
_D1 > _D2
[Equation 2]
_L1 > _L2
Here, D ₁ is the distance from one surface of the housing to the front end, D ₂ is the distance from one surface of the blocking member to the front end, L ₁ is the distance from one surface of the housing to one end of the blocking member, and L ₂ is the distance of the housing Distance from one side to the front end. delete

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