KR102527256B1 - A composition for the prevention, improvement or treatment of sleep disorders or insomnia containing Glehnia littoralis extract - Google Patents
A composition for the prevention, improvement or treatment of sleep disorders or insomnia containing Glehnia littoralis extract Download PDFInfo
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- KR102527256B1 KR102527256B1 KR1020200125727A KR20200125727A KR102527256B1 KR 102527256 B1 KR102527256 B1 KR 102527256B1 KR 1020200125727 A KR1020200125727 A KR 1020200125727A KR 20200125727 A KR20200125727 A KR 20200125727A KR 102527256 B1 KR102527256 B1 KR 102527256B1
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- sleep
- extract
- insomnia
- preventing
- disorder
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- 229920001285 xanthan gum Polymers 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
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- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 description 1
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Abstract
본 발명은 갯방풍(Glehnia littoralis) 추출물을 유효성분으로 함유하는 수면장애 또는 불면증의 예방, 개선 또는 치료용 조성물에 관한 것이다.
본 발명의 갯방풍 추출물은 입면시간의 감소, 수면 지속시간의 증가 및 논렘 수면의 증가 효과가 매우 뛰어나다. 또한, 천연물인 갯방풍으로부터 유래한 것이어서 장기간 사용하여도 인지장애(cognitive impairment), 내성 또는 의존성이 형성되는 부작용이 없다. 따라서, 본 발명의 갯방풍 추출물을 유효성분으로 포함하는 수면장애 예방, 개선 또는 치료용 조성물은 경쟁력 있는 식품 조성물 및 의약 조성물의 제조에 매우 유용하다.The present invention relates to a composition for preventing, improving or treating sleep disorder or insomnia, containing an extract of Glehnia littorali s as an active ingredient.
The gaetbangpung extract of the present invention is very effective in reducing elevation time, increasing sleep duration, and increasing non-REM sleep. In addition, since it is derived from the sea breeze, which is a natural product, there are no side effects such as cognitive impairment, tolerance or dependence even when used for a long time. Therefore, the composition for the prevention, improvement or treatment of sleep disorder comprising the extract of the present invention as an active ingredient is very useful for the preparation of competitive food compositions and pharmaceutical compositions.
Description
본 발명은 갯방풍 추출물을 유효성분으로 함유하는 수면장애 또는 불면증의 예방, 개선 또는 치료용 조성물에 관한 것이다.The present invention relates to a composition for the prevention, improvement or treatment of sleep disorder or insomnia, containing an extract of sea bream as an active ingredient.
수면은 여러 가지 관점에서 정의할 수 있지만 주로 외부 환경을 인식하고 반응하는 능력이 가역적, 반복적, 정상적으로 정지되어 있는 움직이지 않는 상태로 정의한다. 상기 수면은 뇌의 활동에 의해 이루어지지만 다른 신체 부위의 생리학적 변화와도 깊이 연관되어 있다.Sleep can be defined from various perspectives, but it is mainly defined as a state of immobility in which the ability to recognize and respond to the external environment is reversible, repetitive, and normally stopped. The sleep is achieved by brain activity, but is also deeply related to physiological changes in other body parts.
수면장애는 현대인들이 흔히 겪는 문제 중의 하나로서, 인구의 약 20% 이상이 경험한 적이 있거나 앓고 있는 매우 흔한 질환이다. 이러한 수면장애란 건강한 수면을 취하지 못하거나, 충분한 수면을 취하고 있음에도 낮 동안에 각성을 유지하지 못하는 상태, 또는 수면리듬이 흐트러져 있어서 잠자거나 깨어 있을 때 어려움을 겪는 상태를 말한다. 정신적인 문제가 큰 원인이 되기도 하는데 과도한 스트레스와 불안감, 긴장감, 공포 등으로 인해 발생한다.Sleep disorder is one of the common problems of modern people, and it is a very common disease that more than 20% of the population has experienced or suffers from. Such a sleep disorder refers to a state in which a person does not have a sound sleep, does not maintain arousal during the day despite having enough sleep, or has difficulty falling asleep or waking up due to a disturbed sleep rhythm. Mental problems are also a major cause, and it occurs due to excessive stress, anxiety, tension, and fear.
수면장애는 여러 가지 개인적, 사회적 문제를 초래할 수 있으며 학습장애, 능률저하, 교통사고, 안전사고, 정서장애, 사회 적응장애, 결혼생활의 불만족, 그리고 산업재해 등의 원인이 된다. 또한 수면장애를 적절하게 치료하지 않으면 이미 앓고 있는 내과적, 신경과적, 정신과적 질환이 악화되거나 회복이 지연될 수 있고 심근경색증, 뇌졸중 등의 심각한 병을 초래할 수 있다.Sleep disorders can cause various personal and social problems, and cause learning disabilities, reduced performance, traffic accidents, safety accidents, emotional disorders, social adjustment disorders, dissatisfaction in marriage, and industrial accidents. In addition, if sleep disorders are not properly treated, existing medical, neurological, and psychiatric diseases may worsen or recovery may be delayed, and serious diseases such as myocardial infarction and stroke may result.
이러한 수면장애를 치료하기 위하여 중추신경을 가역적으로 억압하여 수면을 유도하고 수면 상태를 유지시키는 화학적 수면제가 사용된다. 종래의 수면제는 마취제와 같은 중추억제작용이 있어 소량으로는 진정작용, 중등량으로는 수면작용을 하지만, 다량으로는 혼수, 마비, 호흡억제작용을 한다. 바르비탈계 약제는 안전성이 낮아 내성과 의존성이 쉽게 일어나고 장기 연용 후 중지하면 악몽 등에 의한 수면장애가 일어나는 문제가 있다.In order to treat these sleep disorders, chemical hypnotics are used that reversibly suppress the central nervous system to induce sleep and maintain the sleep state. Conventional sleeping pills have a central depressant action like an anesthetic, so they have a sedative action in a small amount and a sleeping action in a moderate dose, but a coma, paralysis, and respiratory suppression action in a large amount. Barbital-based drugs have low safety, and tolerance and dependence easily occur, and when they are stopped after long-term continuous use, there is a problem in that sleep disorders such as nightmares occur.
최근에는 유해작용이 적고 항불안 작용이 있는 벤조디아제핀계 약제가 사용되고 있다. 상기 벤조다이아제핀을 포함하여 많은 약물들이 GABAA 수용체에 결합하여 약리작용을 행하고 있고, 벤조다이아제핀 부위에 약물 또는 보조제가 결합하면 GABA에 대한 GABAA 수용체 친화도를 증진시키고, 염소 이온의 세포 내 유입을 증가시켜 불안 완화, 경련 개선, 진정 작용, 및 수면 유도 및 개선 효과를 보인다. GABAA 수용체는 멤브레인 이온 채널을 형성하는 펜타머릭 단백질로서, 진정, 수면, 불안, 근육긴장, 경련, 기억 상실 등의 조절과 밀접한 관련이 있고, 이를 통하여 GABA(감마-아미노부티르산)이 작용하게 된다. 이러한 약물들은 장기간 사용하였을 때 내성 및 의존성이 형성되는 부작용을 동반하며 근육완화, 건망증, 무기력, 혼수상태, 관상혈관 확장, 심경근 차단 등의 증상이 나타나고, 특히 임산부가 사용하면 선천성 기형을 유발할 수도 있다.Recently, benzodiazepine-based drugs with low harmful effects and anti-anxiety effects have been used. Many drugs, including the benzodiazepines, bind to GABA A receptors to exert pharmacological action, and when drugs or adjuvants bind to benzodiazepine sites, the affinity of GABA A receptors for GABA is enhanced, and chloride ions are released into cells. By increasing the influx, it exhibits anxiolytic, convulsive, sedative, and sleep inducing and improving effects. The GABA A receptor is a pentameric protein that forms a membrane ion channel, and is closely related to the regulation of sedation, sleep, anxiety, muscle tension, convulsions, and memory loss, through which GABA (gamma-aminobutyric acid) acts. . When these drugs are used for a long time, they are accompanied by side effects such as tolerance and dependence, and symptoms such as muscle relaxation, forgetfulness, lethargy, coma, coronary vasodilation, and myocardial muscle block appear. .
따라서, 수면제 장기 복용자 및 제한자의 경우 이를 대체할 수 있는 수단의 필요성이 높아지고 있으며, 수면 증진용 건강기능식품에 대한 수요가 증대되고 있는 실정이다.Therefore, in the case of long-term users and restrictive users of sleeping pills, the need for alternative means is increasing, and the demand for health functional foods for sleep enhancement is increasing.
본 발명의 목적은 갯방풍 추출물을 유효성분으로 함유하여 수면장애 또는 불면증을 예방 또는 개선할 수 있는 식품 조성물을 제공하는데 있다.An object of the present invention is to provide a food composition capable of preventing or improving sleep disorder or insomnia by containing an extract of sea bream as an active ingredient.
또한, 본 발명의 다른 목적은 갯방풍 추출물을 유효성분으로 함유하여 수면장애 또는 불면증을 예방 또는 치료할 수 있는 약학 조성물을 제공하는데 있다.In addition, another object of the present invention is to provide a pharmaceutical composition capable of preventing or treating sleep disorder or insomnia by containing an extract of sea bream as an active ingredient.
또한, 본 발명의 다른 목적은 갯방풍 추출물을 유효성분으로 함유하여 수면장애 또는 불면증을 예방 또는 개선할 수 있는 건강기능식품 조성물을 제공하는데 있다.In addition, another object of the present invention is to provide a health functional food composition that can prevent or improve sleep disorder or insomnia by containing the extract of sea bream as an active ingredient.
상기한 목적을 달성하기 위한 본 발명의 수면장애 또는 불면증의 예방 또는 개선용 식품 조성물은 갯방풍(Glehnia littoralis) 추출물을 유효성분으로 포함할 수 있다. To achieve the above object, the food composition for preventing or improving sleep disorder or insomnia of the present invention may contain Glehnia littoralis extract as an active ingredient.
상기 갯방풍 추출물은 물, 탄소수 1 내지 4의 저급알코올 또는 이들의 혼합용매로 추출된 것일 수 있다.The sea bream extract may be extracted with water, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof.
상기 혼합용매는 20 내지 80% 부피%의 메탄올, 에탄올, 부탄올 또는 프로판올 수용액일 수 있으며, 바람직하게는 60 내지 80%의 에탄올 수용액일 수 있다.The mixed solvent may be a 20 to 80% volume % methanol, ethanol, butanol or propanol aqueous solution, preferably a 60 to 80% ethanol aqueous solution.
상기 갯방풍 추출물은 65 내지 95 ℃의 추출온도로 추출된 것일 수 있다.The sea bream extract may be extracted at an extraction temperature of 65 to 95 ° C.
상기 수면장애 또는 불면증은 카페인 투여로 인한 것일 수 있다.The sleep disorder or insomnia may be due to caffeine administration.
상기 수면장애 또는 불면증의 예방 또는 치료는 입면 시간의 감소, 수면 지속 시간의 증가, 또는 논렘 수면의 증가를 위한 것일 수 있다.Prevention or treatment of the sleep disorder or insomnia may be for reducing the sleeping time, increasing the duration of sleep, or increasing nonrem sleep.
상기 식품 조성물의 제형은 분말, 과립, 정제, 캡슐 또는 음료일 수 있다. The dosage form of the food composition may be powder, granule, tablet, capsule or beverage.
상기 식품 조성물은 커피 음료일 수 있다.The food composition may be a coffee beverage.
또한, 상기한 다른 목적을 달성하기 위한 본 발명의 수면장애 또는 불면증의 예방 또는 치료용 약학 조성물은 갯방풍(Glehnia littoralis) 추출물을 유효성분으로 포함할 수 있다. In addition, the pharmaceutical composition for preventing or treating sleep disorder or insomnia of the present invention for achieving the above other object may include a Glehnia littoralis extract as an active ingredient.
또한, 상기한 또 다른 목적을 달성하기 위한 본 발명의 수면장애 예방 또는 개선용 건강기능식품 조성물은 갯방풍(Glehnia littoralis) 추출물을 유효성분으로 포함할 수 있다. In addition, the health functional food composition for preventing or improving sleep disorder of the present invention for achieving another object described above may include a Glehnia littorali s extract as an active ingredient.
본 발명의 갯방풍 추출물은 입면시간의 감소, 수면 지속시간의 증가 및 논렘 수면의 증가 효과가 매우 뛰어나다. 또한, 천연물인 갯방풍으로부터 유래한 것이어서 장기간 사용하여도 인지장애(cognitive impairment), 내성 또는 의존성이 형성되는 부작용이 없다. 따라서, 본 발명의 갯방풍 추출물을 유효성분으로 포함하는 수면장애 예방, 개선 또는 치료용 조성물은 경쟁력 있는 식품 조성물 및 의약 조성물의 제조에 매우 유용하다.The gaetbangpung extract of the present invention is very effective in reducing elevation time, increasing sleep duration, and increasing non-REM sleep. In addition, since it is derived from the sea breeze, which is a natural product, there are no side effects such as cognitive impairment, tolerance or dependence even when used for a long time. Therefore, the composition for the prevention, improvement or treatment of sleep disorder comprising the extract of the present invention as an active ingredient is very useful for the preparation of competitive food compositions and pharmaceutical compositions.
도 1a는 펜토바비탈 수면용량(45 mg/kg, i.p.)을 투여한 마우스의 입면시간에 대한 갯방풍 추출물(KS-093)의 농도별 효과를 비교하여 나타내는 그래프이며, 도 1b는 펜토바비탈 수면용량(45 mg/kg, i.p.)을 투여한 마우스의 수면시간에 대한 갯방풍 추출물(KS-093)의 농도별 효과를 대표적인 수면제인 디아제팜(diazepam)과 비교하여 나타내는 그래프이다. 대조군(5% tween 80-식염수 10 mL/kg), diazepam(2 mg/kg) 및 KS-093(50, 100, 200, 300 mg/kg)를 경구투여(p.o.)하고 45분 후 펜토바비탈을 투여하였다. 각 그래프는 평균값(meansㅁSEM, n=10)을 나타낸다. ***은 대조군과 비교하여 p<0.001에서 유의적 차이가 있음(Tukey's test)을 의미한다. KS-093은 갯방풍 추출물의 약자이다.
도 2a는 수면 구조 분석을 위해 측정된 각 그룹별 단일 마우스의 뇌파(EEG), 근전도도(EMG), 및 hypnograms의 전형적인 예시를 나타낸 그래프이다. 도 2b는 수면구조분석을 통해 마우스의 입면시간에 대한 갯방풍 추출물(KS-093)의 농도별 효과를 시판 수면제인 졸피뎀(zolpidem)과 비교하여 나타낸 그래프이며, 도 2c는 논렘수면(NREMS) 증가 효과에 대한 갯방풍 추출물(KS-093)의 농도별 효과를 시판 수면제인 졸피뎀(zolpidem)과 비교하여 나타내는 그래프이다. Vehicle은 대조군을 나타내고, *은 대조구와 비교하여 p<0.05에서, **은 대조군과 비교하여 p<0.01에서 유의적 차이가 있음(t-test)을 의미한다. KS-093은 갯방풍 추출물의 약자이고, Wake는 wakefulness의 약자이며, NREMS 및 REMS는 각각 non-rapid eye movement sleep 및 rapid eye movement sleep의 약자이다. 또한 EEG 및 EMG는 각각 electroencephalogram 및 electromyogram의 약자이다.
도 3은 24 시간동안의 각 시간대별 논렘수면(NREMS), 렘수면(REMS) 및 각성(Wake)의 변화에 대한 실시예의 갯방풍 추출물(KS-093, 300 mg/kg) 및 졸피뎀(zolpidem, 10 mg/kg)의 효과를 나타내는 그래프이다. Vehicle은 대조군을 나타내고, *은 대조구와 비교하여 p<0.05에서, **은 대조군과 비교하여 p<0.01에서 유의적 차이가 있음(t-test)을 의미한다. KS-093은 갯방풍 추출물의 약자이고, Wake는 wakefulness의 약자이며, NREMS 및 REMS는 각각 non-rapid eye movement sleep 및 rapid eye movement sleep의 약자이다.
도 4는 세부적인 수면구조분석을 위한 mean duration, number of bouts, 및 stage transition 수에 대한 실시예 1의 갯방풍 추출물(KS-093, 300 mg/kg) 및 졸피뎀(zolpidem, 10 mg/kg)의 효과를 나타내는 그래프이다. Vehicle은 대조군을 나타내고, *은 대조구와 비교하여 p<0.05에서, **은 대조군과 비교하여 p<0.01에서 유의적 차이가 있음(t-test)을 의미한다. KS-093은 갯방풍 추출물의 약자이고, Wake(혹은 W)는 wakefulness의 약자이며, NREMS(혹은 N) 및 REMS(혹은 R)는 각각 non-rapid eye movement sleep 및 rapid eye movement sleep의 약자이다.
도 5는 갯방풍 추출물(300 mg/kg) 및 졸피뎀(10 mg/kg)의 duration 별 bouts 수 및 논렘수면에서의 뇌파(EEG, elctroencephalogram) 파워 밀도 및 델타 활성(Delta activity)를 나타낸 그래프이다. Vehicle은 대조군을 나타내고, *은 대조구와 비교하여 p<0.05에서, **은 대조군과 비교하여 p<0.01에서 유의적 차이가 있음(t-test)을 의미한다. KS-093은 갯방풍 추출물의 약자이고, NREMS는 non-rapid eye movement sleep의 약자이다.
도 6a는 카페인 유도 수면장애 마우스 모델에서 펜토바비탈 수면유도 실험을 통하여 입면시간에 대한 실시예 1의 갯방풍 추출물(KS-093)의 농도별 효과를 나타내는 그래프이고, 도 6b는 카페인 유도 수면장애 마우스 모델에서 펜토바비탈 수면유도 실험을 통하여 수면시간에 대한 갯방풍 추출물(KS-093)의 농도별 효과를 나타내는 그래프이다. 일반 대조군(CON; 5% tween 80-식염수 10 mL/kg), 카페인 단독, 카페인 및 실시예, 카페인 및 졸피뎀을 경구투여(p.o.)하였다. 각 그래프는 평균값(meansㅁSEM, n=10)을 나타낸다. # 및 ##은 일반대조군과 비교하여 각각 p<0.05 및 p<0.01에서 유의적 차이가 있음을 의미하며, *, **, 및 ***은 카페인 단독투여군과 비교하여 각각 p<0.05, p<0.01, 및 p<0.001에서 유의적 차이가 있음(Tukey's test)을 의미한다. CON은 일반대조군을 나타내고, KS-093은 갯방풍 추출물의 약자이며, Ca 및 ZPD는 각각 카페인(caffeine) 및 졸피뎀(zolpidem)의 약자이다.
도 7a는 카페인 유도 수면장애 마우스 모델에서 각 그룹별 단일 마우스의 뇌파(EEG), 근전도도(EMG), 및 hypnograms의 전형적인 예시를 나타낸 것이고, 도 7b는 카페인 유도 수면장애 마우스 모델에서, 입면시간, 논렘수면(NREMS) 시간, 렘수면(REMS) 시간에 대한 실시예의 갯방풍 추출물(KS-093)의 효과를 나타내는 그래프이다. 일반 대조군(Vehicle; 5% tween 80-식염수 10 mL/kg), 카페인(25 mg/kg), 카페인(25 mg/kg) + ZPD(10 mg/kg) 및 카페인(25 mg/kg) + 실시예 1의 갯방풍 추출물(300 mg/kg)을 경구투여(p.o.)하였다. 각 그래프는 평균값(meansㅁSEM, n=7)을 나타낸다. **은 대조군과 비교하여 p<0.01에서 유의적 차이가 있음을 의미하고, ##은 카페인 단독투여군과 비교하여 p<0.01에서 유의적 차이가 있음(t-test)을 의미 한다. Vehicle은 대조군을 나타내고, KS-093은 갯방풍 추출물의 약자이며, Wake, NREMS, 및 REMS는 각각 wakefulness, non-rapid eye movement sleep, 및 rapid eye movement sleep의 약자이다. 또한 EEG 및 EMG는 각각 electroencephalogram 및 electromyogram의 약자이다.Figure 1a is a graph showing the effect of each concentration of the sea bream extract (KS-093) on the elevation time of mice administered with pentobarbital sleep dose (45 mg / kg, ip), Figure 1b is pentobarbital This is a graph showing the effect of each concentration of sea bream extract (KS-093) on the sleep time of mice administered with a sleeping dose (45 mg/kg, ip) compared to that of diazepam, a representative sleeping pill. Control group (5% tween 80-
Figure 2a is a graph showing typical examples of electroencephalogram (EEG), electromyography (EMG), and hypnograms of a single mouse for each group measured for sleep structure analysis. Figure 2b is a graph showing the effect of each concentration of sea bream extract (KS-093) on the elevation time of mice through sleep structure analysis compared to zolpidem, a commercially available sleeping pill, Figure 2c is non-REM sleep (NREMS) This is a graph showing the effect of each concentration of sea bream extract (KS-093) on the increasing effect compared to zolpidem, a commercially available sleeping pill. Vehicle represents the control group, * indicates a significant difference at p<0.05 compared to the control group, and ** indicates a significant difference at p<0.01 compared to the control group ( t -test). KS-093 is an abbreviation of seaweed extract, Wake is an abbreviation of wakefulness, and NREMS and REMS are abbreviations of non-rapid eye movement sleep and rapid eye movement sleep, respectively. Also, EEG and EMG are abbreviations of electroencephalogram and electromyogram, respectively.
Figure 3 is an example of the change in non-REM sleep (NREMS), REM sleep (REMS) and wakefulness (Wake) for each time period for 24 hours (KS-093, 300 mg / kg) and zolpidem (zolpidem, It is a graph showing the effect of 10 mg/kg). Vehicle represents the control group, * indicates a significant difference at p<0.05 compared to the control group, and ** indicates a significant difference at p<0.01 compared to the control group ( t -test). KS-093 is an abbreviation of seaweed extract, Wake is an abbreviation of wakefulness, and NREMS and REMS are abbreviations of non-rapid eye movement sleep and rapid eye movement sleep, respectively.
Figure 4 shows the mean duration, number of bouts, and number of stage transitions for detailed water surface structure analysis in Example 1 for the extract (KS-093, 300 mg/kg) and zolpidem (zolpidem, 10 mg/kg). ) is a graph showing the effect of Vehicle represents the control group, * indicates a significant difference at p<0.05 compared to the control group, and ** indicates a significant difference at p<0.01 compared to the control group ( t -test). KS-093 is an abbreviation for seaweed extract, Wake (or W) is an abbreviation for wakefulness, and NREMS (or N) and REMS (or R) are an abbreviation for non-rapid eye movement sleep and rapid eye movement sleep, respectively.
Figure 5 is a graph showing the number of bouts by duration of sea bream extract (300 mg / kg) and zolpidem (10 mg / kg), and the electroencephalogram (EEG) power density and delta activity in non-REM sleep. . Vehicle represents the control group, * indicates a significant difference at p<0.05 compared to the control group, and ** indicates a significant difference at p<0.01 compared to the control group ( t -test). KS-093 is an abbreviation for sea bream extract, and NREMS is an abbreviation for non-rapid eye movement sleep.
Figure 6a is a graph showing the effect of each concentration of the sea bream extract (KS-093) of Example 1 on the elevation time through the pentobarbital sleep induction experiment in the caffeine-induced sleep disorder mouse model, Figure 6b is caffeine-induced sleep disorder It is a graph showing the effect of each concentration of sea bream extract (KS-093) on sleep time through a pentobarbital sleep induction experiment in a mouse model. A general control group (CON; 5% tween 80-
Figure 7a shows typical examples of brain waves (EEG), electromyography (EMG), and hypnograms of a single mouse for each group in a caffeine-induced sleep disorder mouse model, and Figure 7b is a caffeine-induced sleep disorder mouse model, elevation time, Non-REM sleep (NREMS) time, it is a graph showing the effect of the sea breeze extract (KS-093) of the examples for the REM sleep (REMS) time. General control (Vehicle; 5% tween 80-
본 발명은 갯방풍 추출물을 유효성분으로 함유하여 수면장애 또는 불면증을 예방, 개선 또는 치료할 수 있는 조성물에 관한 것이다.The present invention relates to a composition capable of preventing, improving, or treating sleep disorder or insomnia by containing an extract of sea bream as an active ingredient.
상기 수면장애 또는 불면증은 다양한 원인에 의해 발생될 수 있다. 상기 수면장애 또는 불면증은 카페인 투여로 인한 것일 수 있고, 구체적으로 커피, 고카페인 에너지 음료, 콜라, 녹차, 홍차, 코코아 등 카페인이 들어있는 식품의 섭취로 인한 것일 수 있으나, 이에 제한되지 않는다. The sleep disorder or insomnia may be caused by various causes. The sleep disorder or insomnia may be due to caffeine administration, and specifically may be due to consumption of caffeine-containing foods such as coffee, high-caffeine energy drinks, cola, green tea, black tea, and cocoa, but is not limited thereto.
상기 수면장애 또는 불면증의 예방, 개선 또는 치료는 입면 시간(sleep latency)이 감소하는 효과, 수면 지속 시간(sleep duration)이 증가하는 효과 또는 논렘 수면이 증가하는 효과일 수 있으나, 이에 제한되지 않는다.Prevention, improvement or treatment of the sleep disorder or insomnia may be an effect of reducing sleep latency, an effect of increasing sleep duration, or an effect of increasing nonrem sleep, but is not limited thereto.
또한, 상기 수면장애는 기능부전적 수면 기전, 수면 중 생리학적 기능의 이상, 생체시계의 이상, 및 수면과정과 무관한 인자에 의해서 유도된 수면장애를 포함한 다수의 원인으로 인하여 야기되는 붕괴된 수면 패턴을 의미하고, 예를 들어 불면증일 수 있다. 상기 불면증에는 야반 (middle-of-the-night) 불면증, 심야 (late night) 불면증, 수면 시작 후에 장기간 깨어있는 불면증, 수면 유지 불면증, 및 야반 기상 후에 이어지는 불면증을 포함할 수 있다.In addition, the sleep disorder is disrupted sleep caused by a number of causes, including dysfunctional sleep mechanisms, abnormal physiological functions during sleep, abnormalities in the biological clock, and sleep disorders induced by factors unrelated to the sleep process. It means a pattern, and can be, for example, insomnia. The insomnia may include middle-of-the-night insomnia, late night insomnia, prolonged waking insomnia after the onset of sleep, sleep maintenance insomnia, and subsequent insomnia after waking up at night.
하기 실시예에서는, 갯방풍 추출물이 종래에 수면제로서 사용되고 있는 졸피뎀 또는 디아제팜과 비교하여 거의 동일한 수준 또는 향상된 입면 시간의 감소 및 수면 지속 시간의 증가 효과를 나타내며, 논렘 수면시간을 증가시켜줌을 확인하였다. 따라서, 갯방풍 추출물은 수면장애의 예방, 개선 또는 치료용 조성물의 유효성분으로서 사용될 수 있다. 종래의 수면제와는 달리 갯방풍 추출물은 식품으로서 승인되어 있는 인체에 무해한 성분으로서 부작용이 없을 뿐만 아니라 입면 유도 효과 및 수면 연장 효과가 우수하므로, 수면장애의 예방, 개선 또는 치료를 위해 유용하게 사용될 수 있다.In the following examples, it was confirmed that sea bream extract showed almost the same level or improved effect of reducing sleep time and increasing sleep duration compared to zolpidem or diazepam, which were conventionally used as sleeping pills, and increased non-REM sleep time. . Therefore, sea bream extract can be used as an active ingredient of a composition for preventing, improving or treating sleep disorders. Unlike conventional sleeping pills, sea bream extract is a food-approved, harmless ingredient to the human body that has no side effects and has excellent sleep-inducing and sleep-prolonging effects, so it can be usefully used to prevent, improve, or treat sleep disorders. there is.
이하, 본 발명을 상세하게 설명한다.Hereinafter, the present invention will be described in detail.
본 발명의 수면장애 예방, 개선 또는 치료용 조성물은 갯방풍 추출물을 유효성분으로 함유한다.The composition for preventing, improving, or treating sleep disorders of the present invention contains the extract of sea bream as an active ingredient.
갯방풍은 산형과(Umbelliferae)에 속하는 여러해살이 풀로서, 우리나라 해안가 모래땅에 자생하며 일본, 만주, 중국 등지에 분포하는 약용식물이다. 그 뿌리를 북사삼(北沙蔘), 빈방풍(濱防風) 또는 해방풍(海防風)이라 부르며 양음(養陰), 청폐(淸肺), 거담(祛痰), 지해(止咳)의 효능이 있어 전통적으로 항염증, 진해거담약 및 소화기계약으로 사용되어 오고 있다. Gaetbangpung is a perennial plant belonging to the family Umbelliferae, and is a medicinal plant that grows wild in sandy soil along the coast of Korea and is distributed in Japan, Manchuria, and China. Its roots are called northern ginseng (北沙蔘), binbang wind (濱防wind), or liberation wind (海防風), and are said to have positive effects on yin, clearing the lungs, expectoration, and retardation. It has been traditionally used as an anti-inflammatory, antitussive expectorant and digestive contracture.
한편, 갯방풍(Glehnia littoralis)과 명칭이 유사한 식물로서 방풍(ledebouriella seseloides)이 있는데, 상기 방풍은 방풍의 뿌리 및 뿌리줄기를 사용해 만든 약재로서 풍을 막아 준다는 뜻으로 풍병(風病)을 치료하는데 있어서 매우 중요하다. 구체적으로, 상기 방풍은 외감성 두통, 오한, 발열, 전신통, 인후통 등 모든 풍증(風症)에 효과가 있으며, 풍한습의 사지관절동통, 파상풍, 근육경련, 중풍으로 인한 반신불수, 마비동통, 피부가려움증, 버짐 등에 쓰이고, 약리작용으로는 해열, 항염증, 진경, 면역활성화, 항알레르기, 항궤양, 항균, 피부개선균 억제 등이 보고되었다.On the other hand, there is a windbreak ( ledebouriella seseloides ) as a plant with a similar name to the windbreak ( Glehnia littorali s ). very important in doing Specifically, the windshield is effective for all wind symptoms such as external headache, chills, fever, systemic pain, and sore throat, and is effective for all wind symptoms such as wind and damp dampness, tetanus, muscle spasms, paraplegia due to paralysis, paralysis, It is used for skin itching and ringworm, and pharmacological actions have been reported for antipyretic, anti-inflammatory, antispasmodic, immune activation, anti-allergy, anti-ulcer, antibacterial, and suppression of skin improving bacteria.
또한, 갯방풍과 명칭이 유사한 식물로서 식방풍이 있는데, 식방풍의 경우 '방풍나물'로 불리며 약용보다는 식용으로 인기가 높다.In addition, there is Sikbangpung as a plant with a similar name to Gaetbangpung. In the case of Sikbangpung, it is called 'Bangpungnamul' and is more popular for food than medicinal use.
상기 갯방풍, 방풍 및 식방풍은 명칭이 유사하지만, 학명, 서식지 및 형태가 다르고, 유전자가 상이하며, 지표성분들이 다른 별개의 식물이다. 특히, 상기 갯방풍, 방풍 및 식방풍은 지표성분, 또는 생리활성성분에 차이가 있어 상이한 약효를 나타낸다. 구체적으로, 갯방풍에 함유되어 있는 지표성분인 imperatorin 및 panaxydiol은 상기 방풍 및 식방풍에는 함유되어 있지 않은 것으로 확인되었다("방풍, 식방풍 및 해방풍의 감별자료집", 식품의약품안전평가원 참조).The windbreak, windbreak, and windbreak have similar names, but are different plants with different scientific names, habitats and shapes, different genes, and different index components. In particular, the windbreak, windbreak, and windbreak show different medicinal effects due to differences in index components or physiologically active components. Specifically, it was confirmed that imperatorin and panaxydiol, which are index components contained in the windbreak, were not contained in the windbreak and the windbreak (refer to "Discrimination of windbreak, windbreak, and windbreak", Korea Food and Drug Safety Evaluation Institute).
본 발명의 갯방풍은 꽃, 줄기, 잎, 미성숙열매 및 열매 중에서 선택되는 1종 이상의 지상부, 뿌리줄기, 또는 뿌리일 수 있고, 바람직하게는 꽃, 줄기, 잎, 미성숙열매 및 열매 중에서 선택되는 1종 이상의 지상부일 수 있으며, 더욱 바람직하게는 잎 및 줄기 중에서 선택되는 1종 이상의 지상부일 수 있다. 기존에 약재로 사용되던 갯방풍은 갯방풍의 뿌리를 의미하며, "대한민국약전(KP)" 에 등재된 품목도 갯방풍의 뿌리로 등재되어 있다. 그러나, 본 발명은 갯방풍의 지상부를 원료로 하는 것이 수면효능 면에서 더욱 바람직하다.The windbreak of the present invention may be one or more above-ground parts, rhizomes, or roots selected from flowers, stems, leaves, immature fruits and fruits, and preferably one selected from flowers, stems, leaves, immature fruits and fruits. It may be more than one species of aerial parts, more preferably one or more kinds of aerial parts selected from leaves and stems. Gaetbangpung, which was previously used as a medicinal material, means the root of Gaetbangpung, and items listed in the "Korean Pharmacopoeia (KP)" are also listed as the root of Gaetbangpung. However, in the present invention, it is more preferable in terms of sleep effect to use the aerial part of the sea breeze as a raw material.
상기 갯방풍은 추출용매와 1 : 10 내지 30, 바람직하게는 1 : 15 내지 25, 더욱 바람직하게는 1 : 18 내지 22의 중량비로 혼합하여 65 내지 95 ℃, 바람직하게는 70 내지 90 ℃, 더욱 바람직하게는 75 내지 85 ℃에서 1 내지 10시간, 바람직하게는 1 내지 5시간 동안 추출한 후 감압농축을 수행하여 추출물을 제조한다. 상기 갯방풍과 추출용매의 중량비가 상기 범위를 벗어나는 경우에는 추출물에 갯방풍의 유효성분이 적은 양으로 추출될 수 있다.The sea breeze is mixed with the extraction solvent at a weight ratio of 1: 10 to 30, preferably 1: 15 to 25, more preferably 1: 18 to 22, and the temperature is 65 to 95 ℃, preferably 70 to 90 ℃, more Preferably, the extract is prepared by performing extraction at 75 to 85 ° C. for 1 to 10 hours, preferably 1 to 5 hours, and then concentrated under reduced pressure. When the weight ratio of the sea breeze and the extraction solvent is out of the above range, the active ingredient of the sea breeze may be extracted in a small amount in the extract.
상기 각 추출물을 추출하는 추출용매는 물, 탄소수 1 내지 4의 저급알코올 또는 이들의 혼합용매이다. 상기 저급알코올로는 20 내지 80%의 메탄올, 에탄올, 부탄올 또는 프로판올을 들 수 있다.The extraction solvent for extracting each extract is water, lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof. Examples of the lower alcohol include 20 to 80% methanol, ethanol, butanol or propanol.
상기 추출용매로는 특별히 한정하는 것은 아니지만 60 내지 80%의 에탄올 수용액으로 추출된 추출물이 수면장애 또는 불면증의 예방, 개선 또는 치료에 바람직하게 작용한다.The extraction solvent is not particularly limited, but the extract extracted with 60 to 80% aqueous ethanol solution preferably acts to prevent, improve or treat sleep disorders or insomnia.
상기 갯방풍 추출물은 갯방풍을 물, 탄소수 1 내지 4의 저급알코올 또는 이들의 혼합용매로 추출한 추출물을 제2의 유기용매로 재분획한 분획물일 수 있다. 바람직하게는, 갯방풍 추출물은 갯방풍의 에탄올 추출물을 헥산, 에틸아세테이트, 부탄올 또는 물로 재분획한 분획물일 수 있다.The extract of the sea bream may be a fraction obtained by re-fractionating an extract obtained by extracting the sea bream with water, a lower alcohol having 1 to 4 carbon atoms, or a mixed solvent thereof with a second organic solvent. Preferably, the bay wind extract may be a fraction obtained by re-fractionating the ethanol extract of sea bay wind with hexane, ethyl acetate, butanol or water.
본 명세서에서 갯방풍을 언급하면서 사용되는 용어 "추출물"은 추출용매를 처리하여 얻은 조추출물뿐만 아니라 갯방풍 추출물의 가공물도 포함한다. 예를 들어, 갯방풍 추출물은 감압 증류 및 동결 건조 또는 분무 건조 등과 같은 추가적인 과정에 의해 분말 상태로 제조될 수 있다.The term "extract" used herein while referring to the sea breeze includes not only the crude extract obtained by treating the extraction solvent, but also the processed product of the sea breeze extract. For example, sea bream extract can be prepared in a powder state by additional processes such as distillation under reduced pressure and freeze drying or spray drying.
또한, 본 발명의 갯방풍 추출물은 광의로는 갯방풍을 동물에게 투여할 수 있도록 제형화된 갯방풍 가공물, 예컨대, 갯방풍 분말도 포함하는 의미를 갖는다. 비록 본 발명에서 갯방풍으로 실험을 진행하긴 하였으나, 갯방풍 가공물과 같은 형태로도 목적하는 효과를 달성할 수 있음은 당업자라면 예상가능할 것이다.In a broad sense, the extract of the present invention also has a meaning that includes a sea breeze processed product formulated to be administered to animals, such as a sea breeze powder. Although the experiment was conducted with the sea breeze in the present invention, it will be expected by those skilled in the art that the desired effect can be achieved even in the same form as the sea breeze.
한편, 본 명세서에서 용어 "유효성분으로 함유하는"이란 갯방풍 추출물의 효능 또는 활성을 달성하는 데 충분한 양을 포함하는 것을 의미한다. 본 발명의 갯방풍 추출물의 1일 투여량은, 10 내지 1,000 mg/kg일 수 있으며, 바람직하게는 20 내지 500 mg/kg일 수 있고, 더욱 바람직하게는 50 내지 500 mg/kg일 수 있으며, 더욱 바람직하게는 100 내지 500 mg/kg일 수 있고, 더욱 바람직하게는 100 내지 300 mg/kg 또는 200 내지 500 mg/kg일 수 있으며, 더욱 바람직하게는 200 내지 400 mg/kg일 수 있고, 가장 바람직하게는 200 내지 300 mg/kg일 수 있다. 본 발명의 갯방풍 추출물의 투여량의 상기 하한치 미만인 경우에는 수면 효과가 원하는 정도로 나타나지 않을 수 있고, 상기 상한치를 초과하는 경우에는 투여량이 증가하는 만큼 수면 효과가 증가하지 않을 수 있다. 갯방풍 추출물은 천연물로서 과량 투여하여도 인체에 부작용이 없으므로 본 발명의 조성물 내에 포함되는 방풍 추출물의 양적 상한은 당업자가 적절한 범위 내에서 선택하여 실시할 수 있다.On the other hand, in the present specification, the term "contained as an active ingredient" means that it contains a sufficient amount to achieve the efficacy or activity of the sea chestnut extract. The daily dose of the lobster extract of the present invention may be 10 to 1,000 mg/kg, preferably 20 to 500 mg/kg, and more preferably 50 to 500 mg/kg, It may be more preferably 100 to 500 mg/kg, more preferably 100 to 300 mg/kg or 200 to 500 mg/kg, more preferably 200 to 400 mg/kg, and most preferably 200 to 400 mg/kg. It may be preferably 200 to 300 mg/kg. If the dosage of the sea bream extract of the present invention is less than the lower limit, the sleep effect may not appear as desired, and if it exceeds the upper limit, the sleep effect may not increase as much as the dosage increases. As a natural product, there is no side effect on the human body even when administered in excess, so the upper limit of the amount of the extract contained in the composition of the present invention can be selected and carried out by those skilled in the art within an appropriate range.
또한, 본 발명의 갯방풍 추출물은 방풍 추출물, 감태 추출물 또는 쌀겨 추출물 등 기존의 식물 추출물이 각각 500 mg/kg 이상의 농도에서만 유의적인 수면 효과를 나타내는 한편, 식물 추출물로서는 상대적으로 저용량의 농도에서도 유의적인 수면 효과를 나타낼 수 있다.In addition, the gaetbangpung extract of the present invention shows a significant sleep effect only at a concentration of 500 mg / kg or more of existing plant extracts such as windbreak extract, Ecklonia cava extract, or rice bran extract, respectively. May have a sleep effect.
본 발명의 구체적인 일 실시예에서는, 펜토바비탈 수면유도 실험에서, 마우스 모델에 본 발명의 갯방풍 추출물을 투여한 결과 수면 지속 시간의 증가, 또는 논렘 수면의 증가 효과를 나타내어, 수면장애 또는 불면증을 개선하는 효과가 있음을 구체적으로 확인하였다(도 1 내지 도 5 참조). 또한, 카페인 중독에 의한 수면장애를 가지는 마우스 모델에 본 발명의 갯방풍 추출물을 투여한 결과, 카페인 투여에 의한 수면장애를 개선하는 효과가 있음을 구체적으로 확인하였다(도 6 내지 도 7 참조). 이는, 본 발명의 갯방풍 추출물이 수면장애 또는 불면증의 예방, 개선 또는 치료에 유용하게 이용될 수 있음을 시사하는 것이다.In a specific embodiment of the present invention, in a pentobarbital sleep induction experiment, as a result of administering the sea bream extract of the present invention to a mouse model, the effect of increasing sleep duration or non-REM sleep was shown, resulting in sleep disorders or insomnia. It was specifically confirmed that there is an improvement effect (see FIGS. 1 to 5). In addition, as a result of administering the sea bream extract of the present invention to a mouse model having sleep disorders due to caffeine addiction, it was specifically confirmed that there is an effect of improving sleep disorders caused by caffeine administration (see FIGS. 6 to 7). This suggests that the sea bream extract of the present invention can be usefully used for preventing, improving or treating sleep disorders or insomnia.
본 발명의 용어 "예방"이란, 상기 수면장애 또는 불면증을 억제시키거나 또는 지연시키는 모든 것을 의미한다.The term "prevention" of the present invention means anything that suppresses or delays the sleep disorder or insomnia.
본 발명의 용어 "치료"란, 달리 언급되지 않는 한, 상기 수면장애 또는 불면증의 증상을 역전시키거나, 완화시키거나, 그 진행을 억제하거나, 또는 예방하는 것을 의미한다. The term "treatment" of the present invention, unless otherwise stated, means reversing, alleviating, inhibiting the progression of, or preventing the symptoms of the sleep disorder or insomnia.
본 발명의 용어 "개선"이란, 본 발명의 갯방풍 추출물을 포함하는 조성물의 투여로 치료되는 상태와 관련된 파라미터, 예를 들면 증상의 정도를 적어도 감소시키는 것을 의미한다.The term "improvement" of the present invention means at least a decrease in the parameters related to the condition to be treated, for example, the severity of symptoms, by administration of a composition comprising the extract of the present invention.
본 발명에 따른 식품 조성물은 식품학적으로 적합하고 생리학적으로 허용되는 보조제를 사용하여 분말, 과립, 정제, 캡슐 등으로 제제화하여 기능성 식품으로 이용조할 수 있다. 상기 보조제로는 부형제, 붕해제, 감미제, 결합제, 피복제, 팽창제, 윤활제, 활택제 또는 향미제 등을 사용할 수 있다. The food composition according to the present invention may be formulated into a powder, granule, tablet, capsule, etc. using a food-appropriate and physiologically-acceptable auxiliary agent and used as a functional food. As the adjuvant, excipients, disintegrants, sweeteners, binders, coating agents, expanding agents, lubricants, lubricants, or flavoring agents may be used.
또한, 본 발명에 따른 식품 조성물은 예를 들어, 음료류, 알코올 음료류, 과자류, 다이어트바, 유제품, 육류, 초코렛, 피자, 라면, 기타 면류, 껌류, 아이스크림류 등일 수 있다. 바람직하게는 본 발명의 식품 조성물은 커피 음료일 수 있다.In addition, the food composition according to the present invention may be, for example, beverages, alcoholic beverages, confectionery, diet bars, dairy products, meat, chocolate, pizza, ramen, other noodles, chewing gum, ice cream, and the like. Preferably, the food composition of the present invention may be a coffee beverage.
본 발명의 식품 조성물은 유효성분으로서 갯방풍 추출물뿐만 아니라, 식품 제조 시에 통상적으로 첨가되는 성분을 포함할 수 있으며, 예를 들어, 단백질, 탄수화물, 지방, 영양소, 조미제 및 향미제를 포함한다. 상술한 탄수화물의 예는 모노사카라이드, 예를 들어, 포도당, 과당 등; 디사카라이드, 예를 들어 말토스, 슈크로스, 올리고당 등; 및 폴리사카라이드, 예를 들어 덱스트린, 사이클로덱스트린 등과 같은 통상적인 당 및 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 향미제로서 천연 향미제 [타우마틴, 스테비아 추출물 (예를 들어 레바우디오시드 A, 글리시르히진 등]) 및 합성 향미제(사카린, 아스파르탐 등)를 사용할 수 있다. 예컨대, 본 발명의 식품 조성물이 드링크제와 음료류로 제조되는 경우에는 본 발명의 갯방풍 추출물 이외에 구연산, 액상과당, 설탕, 포도당, 초산, 사과산, 과즙, 및 각종 식물 추출액 등을 추가로 포함시킬 수 있다. 상기 식품 조성물의 제형은 분말, 과립, 정제, 캡슐 또는 음료일 수 있다.The food composition of the present invention may include ingredients that are commonly added during food preparation, as well as sea bream extract as an active ingredient, and include, for example, proteins, carbohydrates, fats, nutrients, seasonings, and flavoring agents. . Examples of the aforementioned carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides such as conventional sugars such as dextrins and cyclodextrins and sugar alcohols such as xylitol, sorbitol and erythritol. As flavoring agents, natural flavoring agents [thaumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.]) and synthetic flavoring agents (saccharin, aspartame, etc.) can be used. For example, when the food composition of the present invention is made into drinks and beverages, citric acid, high fructose corn syrup, sugar, glucose, acetic acid, malic acid, fruit juice, and various plant extracts may be further included in addition to the seaweed extract of the present invention. . The dosage form of the food composition may be powder, granule, tablet, capsule or beverage.
상기 식품 조성물에서 상기 갯방풍 추출물의 함량은 0.001 내지 5 중량%, 바람직하게는 0.001 내지 1 중량%, 더욱 바람직하게는 0.001 내지 0.5 중량%, 더욱 바람직하게는 0.002 내지 0.3 중량%, 더욱 바람직하게는 0.005 내지 0.3 중량%, 더욱 바람직하게는 0.01 내지 0.2 중량%, 더욱 바람직하게는 0.02 내지 0.1 중량%일 수 있다. The content of the sea bream extract in the food composition is 0.001 to 5% by weight, preferably 0.001 to 1% by weight, more preferably 0.001 to 0.5% by weight, more preferably 0.002 to 0.3% by weight, still more preferably 0.005 to 0.3% by weight, more preferably 0.01 to 0.2% by weight, more preferably 0.02 to 0.1% by weight.
또한, 본 발명은 갯방풍 추출물을 유효성분으로 함유하는 수면장애의 예방 또는 치료용 약학 조성물을 제공한다.In addition, the present invention provides a pharmaceutical composition for the prevention or treatment of sleep disorders containing the extract of sea bream as an active ingredient.
본 발명의 약학 조성물은 상기 유효 성분 이외에 약학적으로 적합하고 생리학적으로 허용되는 보조제를 사용하여 제조될 수 있으며, 상기 보조제로는 부형제, 붕해제, 감미제, 결합제, 피복제, 팽창제, 윤활제, 활택제 또는 향미제 등을 사용할 수 있다.The pharmaceutical composition of the present invention can be prepared using pharmaceutically suitable and physiologically acceptable adjuvants in addition to the above active ingredients, and the adjuvants include excipients, disintegrants, sweeteners, binders, coating agents, expanding agents, lubricants, and lubricants. agents or flavoring agents may be used.
상기 약학 조성물은 투여를 위해서 상기 기재한 유효 성분 이외에 추가로 약제학적으로 허용 가능한 담체를 1종 이상 포함하여 약학 조성물로 바람직하게 제제화할 수 있다.The pharmaceutical composition may be preferably formulated as a pharmaceutical composition by including one or more pharmaceutically acceptable carriers in addition to the above-described active ingredients for administration.
상기 약학 조성물의 제제 형태는 과립제, 산제, 정제, 피복정, 캡슐제, 좌제, 액제, 시럽, 즙, 현탁제, 유제, 점적제 또는 주사 가능한 액제 등이 될 수 있다. 예를 들어, 정제 또는 캡슐제의 형태로의 제제화를 위해, 유효 성분은 에탄올, 글리세롤, 물 등과 같은 경구, 무독성의 약학적으로 허용 가능한 불활성 담체와 결합될 수 있다. 또한, 원하거나 필요한 경우, 적합한 결합제, 윤활제, 붕해제 및 발색제 또한 혼합물로 포함될 수 있다. 적합한 결합제는 이에 제한되는 것은 아니나, 녹말, 젤라틴, 글루코스 또는 베타-락토오스와 같은 천연 당, 옥수수 감미제, 아카시아, 트래커캔스 또는 소듐올레이트와 같은 천연 및 합성 검, 소듐 스테아레이트, 마그네슘 스테아레이트, 소듐 벤조에이트, 소듐 아세테이트, 소듐 클로라이드 등을 포함한다. 붕해제는 이에 제한되는 것은 아니나, 녹말, 메틸 셀룰로스, 아가, 벤토니트, 잔탄 검 등을 포함한다.Formulations of the pharmaceutical composition may be granules, powders, tablets, coated tablets, capsules, suppositories, solutions, syrups, juices, suspensions, emulsions, drops, or injectable solutions. For example, for formulation in the form of tablets or capsules, the active ingredient may be combined with an oral, non-toxic, pharmaceutically acceptable inert carrier such as ethanol, glycerol, water, and the like. In addition, if desired or necessary, suitable binders, lubricants, disintegrants and coloring agents may also be included in the mixture. Suitable binders include, but are not limited to, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tracacanth or sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride, and the like. Disintegrants include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum, and the like.
액상 용액으로 제제화되는 조성물에 있어서 허용 가능한 약제학적 담체로는, 멸균 및 생체에 적합한 것으로서, 식염수, 멸균수, 링거액, 완충 식염수, 알부민 주사용액, 덱스트로즈 용액, 말토 덱스트린 용액, 글리세롤, 에탄올 및 이들 성분 중 1 성분 이상을 혼합하여 사용할 수 있으며, 필요에 따라 항산화제, 완충액, 정균제 등 다른 통상의 첨가제를 첨가할 수 있다. 또한 희석제, 분산제, 계면활성제, 결합제 및 윤활제를 부가적으로 첨가하여 수용액, 현탁액, 유탁액 등과 같은 주사용 제형, 환약, 캡슐, 과립 또는 정제로 제제화할 수 있다.In compositions formulated as liquid solutions, acceptable pharmaceutical carriers are sterile and biocompatible, and include saline, sterile water, Ringer's solution, buffered saline, albumin injection solution, dextrose solution, maltodextrin solution, glycerol, ethanol and One or more of these components may be mixed and used, and other conventional additives such as antioxidants, buffers, and bacteriostatic agents may be added if necessary. In addition, diluents, dispersants, surfactants, binders, and lubricants may be additionally added to prepare formulations for injections such as aqueous solutions, suspensions, and emulsions, pills, capsules, granules, or tablets.
더 나아가 해당분야의 적절한 방법으로 Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA에 개시되어 있는 방법을 이용하여 각 질환에 따라 또는 성분에 따라 바람직하게 제제화할 수 있다.Furthermore, using a method disclosed in Remington's Pharmaceutical Science, Mack Publishing Company, Easton PA as an appropriate method in the field, it can be preferably formulated according to each disease or component.
본 발명의 약학 조성물은 경구 또는 비경구로 투여할 수 있고, 비경구 투여인 경우에는 정맥내 주입, 피하 주입, 근육 주입, 복강 주입, 경피 투여 등으로 투여할 수 있으며, 바람직하게는 경구 투여이다.The pharmaceutical composition of the present invention can be administered orally or parenterally, and in the case of parenteral administration, it can be administered by intravenous injection, subcutaneous injection, intramuscular injection, intraperitoneal injection, transdermal administration, etc., preferably oral administration.
본 발명의 약학 조성물의 적합한 투여량은 제제화 방법, 투여 방식, 환자의 연령, 체중, 성, 병적 상태, 음식, 투여 시간, 투여 경로, 배설 속도 및 반응 감응성과 같은 요인들에 의해 다양하며, 보통으로 숙련된 의사는 소망하는 치료 또는 예방에 효과적인 투여량을 용이하게 결정 및 처방할 수 있다. 본 발명의 바람직한 구현예에 따르면, 본 발명의 약학 조성물의 1일 투여량은 0.001-10 g/㎏일 수 있다.The suitable dosage of the pharmaceutical composition of the present invention varies depending on factors such as formulation method, administration method, patient's age, weight, sex, medical condition, food, administration time, administration route, excretion rate and reaction sensitivity, usually This allows the skilled physician to readily determine and prescribe dosages effective for the desired treatment or prophylaxis. According to a preferred embodiment of the present invention, the daily dosage of the pharmaceutical composition of the present invention may be 0.001-10 g/kg.
본 발명의 약학 조성물은 약제학적으로 허용되는 담체 및/또는 부형제를 이용하여 제제화함으로써 단위 용량 형태로 제조되거나 또는 다용량 용기 내에 내입시켜 제조될 수 있다. 이때 제형은 오일 또는 수성 매질중의 용액, 현탁액 또는 유화액 형태이거나 엑스제, 분말제, 과립제, 정제 또는 캅셀제 형태일 수도 있으며, 분산제 또는 안정화제를 추가적으로 포함할 수 있다. The pharmaceutical composition of the present invention may be prepared in a unit dose form by formulation using a pharmaceutically acceptable carrier and/or excipient, or prepared by putting it into a multi-dose container. In this case, the formulation may be in the form of a solution, suspension or emulsion in an oil or aqueous medium, or may be in the form of an extract, powder, granule, tablet or capsule, and may additionally contain a dispersing agent or stabilizer.
또한, 본 발명은 상기 갯방풍 추출물을 유효성분으로 포함하는 수면장애의 예방, 개선 또는 치료용 건강기능식품 조성물을 제공한다. 건강기능식품이란, 갯방풍 추출물을 다양한 식품 소재에 첨가하거나, 캡슐화, 분말화, 현탁액 등으로 제조한 식품으로, 이를 섭취할 경우 건강상 특정한 효과를 가져오는 것을 의미하나, 일반 약품과는 달리 식품을 원료로 하여 약품의 장기 복용시 발생할 수 있는 부작용 등이 없는 장점이 있다. 이와 같이 하여 얻어지는 본 발명의 건강기능식품은, 일상적으로 섭취하는 것이 가능하기 때문에 매우 유용하다. 이와 같은 건강기능식품에 있어서의 갯방풍 추출물의 첨가량은, 대상인 건강기능식품의 종류에 따라 달라 일률적으로 규정할 수 없지만, 식품 본래의 맛을 손상시키지 않는 범위에서 첨가하면 되며, 대상 식품에 대하여 통상 0.01 내지 50 중량%, 바람직하기로는 0.1 내지 20 중량%의 범위이다. 또한, 환제, 과립제, 정제 또는 캡슐제 형태의 건강기능식품의 경우에는 통상 0.1 내지 100 중량% 바람직하기로는 0.5 내지 80 중량%의 범위에서 첨가하면 된다. 한 구체예에서, 본 발명의 건강기능식품은 분말, 과립, 정제, 캡슐 또는 음료의 형태일 수 있다.In addition, the present invention provides a health functional food composition for the prevention, improvement or treatment of sleep disorders, comprising the extract of sea bream as an active ingredient. Health functional food refers to a food made by adding turmeric extract to various food materials, encapsulating, powdering, or suspension. As a raw material, it has the advantage of not having side effects that may occur when taking drugs for a long time. The health functional food of the present invention obtained in this way is very useful because it can be consumed on a daily basis. The addition amount of turmeric extract in such a health functional food cannot be uniformly defined depending on the type of target health functional food, but it can be added within a range that does not impair the original taste of the food. It ranges from 0.01 to 50% by weight, preferably from 0.1 to 20% by weight. In addition, in the case of health functional foods in the form of pills, granules, tablets or capsules, it is usually added in the range of 0.1 to 100% by weight, preferably 0.5 to 80% by weight. In one embodiment, the health functional food of the present invention may be in the form of a powder, granule, tablet, capsule or beverage.
또한, 본 발명은 수면장애의 예방, 개선 또는 치료용 의약 또는 식품의 제조를 위한 갯방풍 추출물의 용도를 제공한다. 상기한 바와 같이 갯방풍 추출물은 수면장애 또는 불면증의 예방, 개선 또는 치료를 위한 용도로 이용될 수 있다.In addition, the present invention provides a use of the extract of the sea bream for the manufacture of a medicine or food for preventing, improving or treating sleep disorders. As described above, sea bream extract can be used for the prevention, improvement or treatment of sleep disorders or insomnia.
또한, 본 발명은 포유동물에게 유효량의 갯방풍 추출물을 투여하는 것을 포함하는 수면장애 또는 불면증의 예방, 개선 또는 치료 방법을 제공한다.In addition, the present invention provides a method for preventing, improving or treating sleep disorder or insomnia, which comprises administering an effective amount of an effective amount of an extract to a mammal.
여기에서 사용된 용어 "포유동물"은 치료, 관찰 또는 실험의 대상인 포유동물을 말하며, 바람직하게는 인간을 말한다.As used herein, the term "mammal" refers to a mammal that is a subject of treatment, observation or experimentation, and preferably refers to a human.
여기에서 사용된 용어 "유효량"은 연구자, 수의사, 의사 또는 기타 임상의에 의해 생각되는 조직계, 동물 또는 인간에서 생물학적 또는 의학적 반응을 유도하는 유효 성분 또는 약학적 조성물의 양을 의미하는 것으로, 이는 해당 질환 또는 장애의 증상의 완화를 유도하는 양을 포함한다. 본 발명의 유효 성분에 대한 유효량 및 투여횟수는 원하는 효과에 따라 변화될 수 있다. 그러므로, 투여될 최적의 투여량은 당업자에 의해 쉽게 결정될 수 있으며, 질환의 종류, 질환의 중증도, 조성물에 함유된 유효성분 및 다른 성분의 함량, 제형의 종류, 및 환자의 연령, 체중, 일반 건강 상태, 성별 및 식이, 투여 시간, 투여 경로 및 조성물의 분비율, 치료기간, 동시 사용되는 약물을 비롯한 다양한 인자에 따라 조절될 수 있다. 본 발명의 예방, 개선 또는 치료 방법에 있어서, 성인의 경우, 갯방풍 추출물을 1일 1회 내지 수회 투여시, 0.001 g/kg 내지 10 g/kg의 용량으로 투여하는 것이 바람직하다.As used herein, the term "effective amount" means an amount of an active ingredient or pharmaceutical composition that induces a biological or medical response in a tissue system, animal or human, which is considered by a researcher, veterinarian, physician or other clinician, which is An amount that induces relief of the symptoms of a disease or disorder is included. The effective amount and frequency of administration of the active ingredient of the present invention can be varied depending on the desired effect. Therefore, the optimal dose to be administered can be easily determined by those skilled in the art, and the type of disease, the severity of the disease, the content of the active ingredient and other ingredients contained in the composition, the type of formulation, and the patient's age, weight, and general health Condition, sex and diet, administration time, administration route and secretion rate of the composition, treatment period, can be adjusted according to various factors including drugs used simultaneously. In the prevention, improvement or treatment method of the present invention, in the case of adults, it is preferable to administer the extract of sea bream at a dose of 0.001 g/kg to 10 g/kg when administered once to several times a day.
본 발명의 치료방법에서 갯방풍 추출물을 유효 성분으로 포함하는 조성물은 경구, 직장, 정맥내, 동맥내, 복강내, 근육내, 흉골내, 경피, 국소, 안구내 또는 피내 경로를 통해 통상적인 방식으로 투여할 수 있다.In the treatment method of the present invention, the composition containing the extract of sea bream as an active ingredient is conventionally administered through oral, rectal, intravenous, intraarterial, intraperitoneal, intramuscular, intrasternal, transdermal, topical, intraocular or intradermal routes. can be administered with
이하, 본 발명에 따른 갯방풍 추출물을 유효성분으로 포함하는 수면장애 또는 불면증의 예방, 개선 또는 치료용 조성물에 대해 실시예 및 비교예를 들어 상세히 설명하기로 한다.Hereinafter, a composition for preventing, improving, or treating sleep disorder or insomnia, including the extract of sea bream according to the present invention as an active ingredient, will be described in detail with Examples and Comparative Examples.
<실시예><Example>
실시예 1. 갯방풍 에탄올 추출물(KS-093)Example 1. Sea breeze ethanol extract (KS-093)
국내산 갯방풍 지상부(잎 및 줄기)를 동결건조한 분쇄물과 70% 에탄올 수용액을 1 : 20의 중량비로 혼합한 후 80 ℃에서 3시간 동안 추출하여 갯방풍 에탄올 추출물을 수득하였다. 상기 수득된 추출물은 여과 및 감압 농축을 거쳐 동결건조로 분말화하여 이용하였다.After mixing the freeze-dried ground parts (leaves and stems) of domestic sphagnum japonica with a 70% aqueous ethanol solution at a weight ratio of 1:20, extraction was performed at 80 ° C. for 3 hours to obtain an ethanol extract of sphagnum sphagnum. The obtained extract was used after being pulverized by lyophilization through filtration and concentration under reduced pressure.
<시험방법><Test method>
실험 동물laboratory animals
ICR 마우스(18-22 g, 웅성) 및 C57BL/6N 마우스 (28-30 g, 웅성)는 ㈜코아텍(경기, 평택)에서 분양받아 실험동물용 사육상자에 일주일간 적응시킨 후 실험에 사용하였다. 동물의 사육은 온도 23ㅁ 1℃, 습도 55ㅁ 5%, 명-암주기(명: 오전 7시-오후 7시), 조도 3000 Lux의 조건하에 이루어졌으며, 사료 및 음수는 자유 급여하였다. 모든 동물들은 한국식품연구원 동물관리위원회(KFRI-IACUC, Korea Food Research Institutional Animal Care and Use Committee)의 실험동물 사용지침에 따라 관리하였다.ICR mice (18-22 g, male) and C57BL/6N mice (28-30 g, male) were purchased from Coretech Co., Ltd. (Pyeongtaek, Gyeonggi-do) and used in the experiment after adapting to the experimental animal breeding box for a week. . Breeding of the animals was carried out under the conditions of temperature 23ㅁ 1℃, humidity 55ㅁ 5%, light-dark cycle (light: 7:00 AM-7:00 PM), illumination intensity 3000 Lux, and feed and drinking water were freely fed. All animals were managed according to the guidelines for use of laboratory animals of the Korea Food Research Institutional Animal Care and Use Committee (KFRI-IACUC).
펜토바비탈 수면유도 실험(Pentobarbital-induced sleep test)Pentobarbital-induced sleep test
펜토바비탈 수면유도 실험은 오후 1시에서 5시 사이의 일정한 시간 내에 진행하였으며, 그룹 당 10 마리(n=10)의 마우스를 실험 전 24시간 동안 절식시킨 후 사용하였다. 모든 시료는 5% tween 80-식염수 용액을 이용하여 제조하였고 펜토바비탈 투여 45분 전에 마우스에 경구투여(p.o.)하였다. 일반 대조군 실험군은 5% tween 80-식염수 수용액을 10 mg/kg의 농도로 처리하였으며, 디아제팜(diazepam, ㈜명인제약)은 대표적인 수면제로서 추출물의 수면증진 효과를 비교하기 위하여 양성 대조군 약물로 사용하였다. 펜토바비탈((주)한림제약)은 실험 디자인에 따라 45 mg/kg(hypnotic dosage)의 농도로 복강주사(i.p.)를 통해 투여하였다. 펜토바비탈 처리 후 각각의 개체를 독립된 공간에 옮겨서 입면시간(sleep latency)과 수면시간(sleep duration)을 측정하였다. 입면시간은 펜토바비탈을 복강주사한 후 정반사(righting reflex)를 1 분 이상 상실할 때까지의 경과시간으로 설정하였고, 수면시간은 다시 정반사를 회복할 때까지의 시간으로 설정하였다. 펜토바비탈 투여 후 10분이 지나도 수면행동을 보이지 않은 마우스는 실험에서 제외하였다.The pentobarbital sleep induction experiment was conducted within a certain time between 1:00 pm and 5:00 pm, and 10 mice per group (n = 10) were used after fasting for 24 hours before the experiment. All samples were prepared using 5% tween 80-saline solution and orally administered (p.o.) to mice 45 minutes before administration of pentobarbital. The general control group was treated with 5% tween 80-saline solution at a concentration of 10 mg/kg, and diazepam (Myeongin Pharmaceutical Co., Ltd.) was used as a positive control drug to compare the sleep enhancing effect of the extract as a representative sleeping pill. Pentobarbital (Hanlim Pharmaceutical Co., Ltd.) was administered through intraperitoneal injection (i.p.) at a concentration of 45 mg/kg (hypnotic dosage) according to the experimental design. After pentobarbital treatment, each object was moved to an independent space to measure sleep latency and sleep duration. The elevation time was set to the elapsed time until the righting reflex was lost for 1 minute or more after intraperitoneal injection of pentobarbital, and the sleep time was set to the time until the righting reflex was restored again. Mice that did not show sleep behavior even after 10 minutes after pentobarbital administration were excluded from the experiment.
수면구조 분석Sleep structure analysis
C57BL/6N 마우스를 1주일간 적응시킨 후 뇌파(Electroencephalogram, EEG) 및 근전도도(Electromyogram, EMG) 측정을 위해 전극 삽입 수술을 실시하였다. 마우스를 pentobarbital(50 mg/kg, i.p.)로 마취시키고 뇌정위기(stereotaxic instrument)에 두부를 고정시켰다. 두부 피하 결합조직을 절개한 후 EEG 및 EMG 측정을 위하여 Mouse EEG/EMG Headmount (Pinnacle Technology Inc, Oregon, USA)를 삽입하였다. 이후 dental cement로 고정 후 수술부위의 소독 및 항생제 투여를 3일 동안 실시하여 수술로 인한 염증을 예방하였으며, 7일간 회복기간을 두었다. 뇌파측정환경에 적응시키기 위하여 측정 4일 전부터 recording 장치를 연결하여 본 실험과정에 순응하도록 유도하였다. EEG 및 EMG는 시료 경구투여 후 5분간 안정화 시킨 다음 PAL-8200 series (Pinnacle Technology Inc, Oregon, USA)를 이용하여 17:00부터 24시간동안 측정하였다. EEG 및 EMG의 sampling rate는 200 Hz로 설정하고(epoch time: 10 s), EEG는 0.1-25 Hz, EMG는 10-100 Hz의 필터 영역을 설정하여 데이터를 기록하였다. 수면구조 분석은 fast Fourier transform (FFT) 알고리즘에 의해 수행되었으며, SleepSign 프로그램(Ver. 3.0, Kissei Comtec, Nagono, Japan)을 이용하였다. 분석결과는 Wake(wakefulness), REMS (rapid eye movement sleep, theta band: 6-10 Hz), NREMS (non-rapid eye movement sleep, delta band: 0.65-4 Hz)으로 구분하여 나타내었고, Sleep latency(입면시간)는 10초 epoch 단위의 NREM 수면이 12번 이상 연속적으로 나타나는데 걸리는 시간으로 설정하였다.After adapting the C57BL/6N mice for 1 week, electrode insertion was performed to measure the EEG and electromyogram (EMG). Mice were anesthetized with pentobarbital (50 mg/kg, i.p.) and head-fixed in a stereotaxic instrument. After incising the head subcutaneous connective tissue, a Mouse EEG/EMG Headmount (Pinnacle Technology Inc, Oregon, USA) was inserted for EEG and EMG measurement. After fixation with dental cement, disinfection of the surgical site and administration of antibiotics were performed for 3 days to prevent inflammation caused by surgery, and a 7-day recovery period was allowed. In order to adapt to the EEG measurement environment, the recording device was connected 4 days before the measurement to induce adaptation to this experimental process. EEG and EMG were measured for 24 hours from 17:00 using a PAL-8200 series (Pinnacle Technology Inc, Oregon, USA) after stabilization for 5 minutes after oral administration of the sample. The sampling rate of EEG and EMG was set to 200 Hz (epoch time: 10 s), and the data was recorded by setting the filter area of 0.1-25 Hz for EEG and 10-100 Hz for EMG. The sleep structure analysis was performed by the fast Fourier transform (FFT) algorithm, and the SleepSign program (Ver. 3.0, Kissei Comtec, Nagono, Japan) was used. The analysis results were divided into wake (wakefulness), REMS (rapid eye movement sleep, theta band: 6-10 Hz), and NREMS (non-rapid eye movement sleep, delta band: 0.65-4 Hz), and sleep latency ( Elevation time) was set as the time required for 10-second epoch units of NREM sleep to appear more than 12 consecutively.
<시험예><Test Example>
시험예 1: 실시예의 갯방풍 추출물(KS-093)을 이용한 펜토바비탈 수면 유도 실험Test Example 1: Pentobarbital sleep induction experiment using the example of sea bream extract (KS-093)
펜토바비탈 수면유도 실험을 통해 실시예 1의 갯방풍 추출물(KS-093)이 어떠한 수면 효능을 나타내는지를 확인하고자 하였다. 이를 위하여, 마우스를 각 군당 10마리씩 6그룹으로 나누어 준비하였다. 일반대조군은 5% tween 80-saline을 투여하였고 실시예 1의 갯방풍 추출물(KS-093)을 50, 100, 200 및 300 mg/kg의 농도로 경구투여(p.o.)한 후 펜토바비탈 (45 mg/kg, i.p.)에 의한 수면유도 효과를 분석하였다. Through a pentobarbital sleep induction experiment, it was attempted to confirm what kind of sleep effect the sea bream extract (KS-093) of Example 1 exhibits. To this end, the mice were prepared by dividing them into 6 groups of 10 mice per group. The general control group was administered with 5% tween 80-saline, and after oral administration (p.o.) of the extract (KS-093) of Example 1 at concentrations of 50, 100, 200 and 300 mg/kg, pentobarbital (45 The sleep induction effect by mg/kg, i.p.) was analyzed.
그 결과, 실시예 1의 갯방풍 추출물(KS-093)은 식물 추출물로서는 상대적으로 저용량인 200 mg/kg 및 300 mg/kg의 농도에서 유의적인 수면 지속 시간의 증가 효과를 나타내었다(도 1 참조).As a result, the sea bream extract (KS-093) of Example 1 showed a significant increase in sleep duration at concentrations of 200 mg/kg and 300 mg/kg, which are relatively low doses for a plant extract (see FIG. 1 ).
시험예 2: 실시예의 갯방풍 추출물(KS-093)을 이용한 수면 구조 분석Test Example 2: Water surface structure analysis using the example of sea bream extract (KS-093)
실시예 1의 갯방풍 추출물(KS-093)의 수면의 양 및 질을 평가하기 위해 마우스의 수면 구조를 분석하고자 하였고, 이를 위해 각 군당 8 마리씩 4그룹의 마우스를 준비하였다. 또한, 본 발명의 갯방풍 추출물(KS-093)의 농도별 수면 효과와 시판 수면제인 졸피뎀(zolpidem)과의 효능을 비교하고자 하였다.In order to evaluate the amount and quality of sleep of the sea bream extract (KS-093) of Example 1, the sleep structure of mice was analyzed. For this purpose, 4 groups of mice, 8 mice in each group, were prepared. In addition, it was intended to compare the sleeping effect of the present invention's sea bream extract (KS-093) by concentration and the efficacy with zolpidem, a commercially available sleeping pill.
수면 구조 분석 결과, 본 발명의 갯방풍 추출물(KS-093)은 농도 의존적으로 논렘 수면을 증가시켰으며, 모든 농도에서 유의적으로 입면시간을 감소시켰다. 또한, 갯방풍 추출물(KS-093)을 300 mg/kg 농도로 투여한 경우 졸피뎀(zolpidem, 10 mg/kg)과 유사한 수면 효과를 나타내었다. 한편, 모든 실험군에서 REM 수면의 변화는 나타나지 않았다(도 2 b 및 c 참조).As a result of the sleep structure analysis, the sea bream extract (KS-093) of the present invention increased nonrem sleep in a concentration-dependent manner, and significantly reduced the elevation time at all concentrations. In addition, when 300 mg/kg of sea bream extract (KS-093) was administered, it showed a sleep effect similar to that of zolpidem (10 mg/kg). On the other hand, no change in REM sleep was observed in all experimental groups (see FIGS. 2 b and c).
또한, 실시예 1의 갯방풍 추출물(KS-093, 300 mg/kg) 및 졸피뎀(zolpidem 10 mg/kg)을 각각 경구투여한 후 C57BL/6N mice의 뇌파를 측정하는 동안 매 시간별 Wake, NREM, 및 REM 수면시간의 변화를 도 3에 나타내었다. 도 3을 살펴보면, 갯방풍 추출물(KS-093)은 경구 투여 후 처음 2시간 동안 NREM 수면의 유의적인 증가를 나타내었고(도 3a 참조), 양성대조군인 졸피뎀(zolpidem)은 대조군(Vehicle) 보다 5시간 동안 NREM 수면의 유의적인 증가를 보였다(도 3b 참조). 이러한 결과로부터, 갯방풍 추출물(KS-093)은 수면 초반에 수면증진효과를 나타낸 후에는 자연적인 수면을 유도한 반면, 수면제인 졸피뎀(zolpidem)은 갯방풍 추출물(KS-093)과 같이 수면 초반에 수면증진효과를 나타내었으나 그 효과가 지나치게 장시간 동안 지속되어, 수면제의 전형적인 부작용을 유발하게 된 것을 확인할 수 있었다. In addition, after oral administration of the sea bream extract (KS-093, 300 mg/kg) and zolpidem (
또한, 갯방풍 추출물(KS-093, 300 mg/kg) 및 졸피뎀(zolpidem, 10 mg/kg)의 경구 투여로 인한 마우스의 sleep-wake profile의 변화를 더욱 구체적으로 파악하기 위해 mean duration, number of bouts, stage transition number 및 episode number를 분석하였다. 갯방풍 추출물(KS-093)과 졸피뎀(zolpidem)의 경구투여에 의해 Wake의 mean duration이 유의적으로 감소하였고 NREMS과 REMS은 변화가 없었다(도 4a 참조).In addition, in order to more specifically identify changes in the sleep-wake profile of mice caused by oral administration of sea bream extract (KS-093, 300 mg/kg) and zolpidem (zolpidem, 10 mg/kg), mean duration, number of blows, stage transition number and episode number were analyzed. The mean duration of wake was significantly decreased by oral administration of sea bream extract (KS-093) and zolpidem, and there was no change in NREMS and REMS (see Fig. 4a).
또한, 갯방풍 추출물(KS-093)과 졸피뎀(zolpidem)은 NREM과 Wake의 bouts의 수를 유의적으로 증가시켰고(도 4b 참조), Wake에서 NREMS으로 그리고 NREMS에서 Wake로 변환되는 횟수 또한 유의적으로 증가시켰다(도 4c 참조). 게다가 갯방풍 추출물(KS-093)과 졸피뎀(zolpidem)에 의해 각각 10-30초 및 10-120초 동안 NREMS를 유지한 episode 수가 유의적으로 증가하였다(도 5a 참조). 이러한 경향은 갯방풍 추출물(KS-093)과 졸피뎀(zolpidem)의 투여가 마우스의 각성 유지를 방해한다는 것을 의미하며, 이는 곧 수면을 증진시켰다는 것을 의미한다.In addition, sea bream extract (KS-093) and zolpidem significantly increased the number of bouts of NREM and Wake (see Fig. 4b), and the number of bouts from Wake to NREMS and from NREMS to Wake was also significant. increased progressively (see Fig. 4c). In addition, the number of episodes in which NREMS was maintained for 10-30 seconds and 10-120 seconds, respectively, was significantly increased by the use of sea bream extract (KS-093) and zolpidem (see Fig. 5a). This tendency means that the administration of sea bream extract (KS-093) and zolpidem interfered with the maintenance of wakefulness of the mice, which in turn improved sleep.
또한, 논렘 수면 중 델타 활성(delta activity)의 변화는 수면의 질과 깊이의 생리적인 지표라 할 수 있다[Behav. Brain Res. 210, 5456. Huang et al., 2010]. 또한 졸피뎀(zolpidem)은 논렘 수면을 증가시키고 논렘 수면 중의 델타 파워를 감소시킨다고 보고된 바 있다[PNAS. 101, 3674-3679. Kopp et al., 2004]. 이러한 전형적인 졸피뎀(zolpidem)의 특성은 본 연구에서도 뚜렷하게 나타났다. 예상대로 졸피뎀(zolpidem)은 논렘 수면 중 델타 활성을 유의적(p<0.01)으로 감소시켰으며, 갯방풍 추출물(KS-093)의 델타 활성은 유의적인 변화가 나타나지 않았다(도 5b 참조). 이러한 결과는 갯방풍 추출물(KS-093)이 생리적인 수면에 영향을 끼치지 않고 NREM 수면을 증가시킨다는 것을 의미한다. In addition, changes in delta activity during non-REM sleep can be said to be a physiological indicator of sleep quality and depth [Behav. Brain Res. 210, 5456. Huang et al., 2010]. It has also been reported that zolpidem increases non-REM sleep and decreases delta power during non-REM sleep [PNAS. 101, 3674-3679. Kopp et al., 2004]. These typical characteristics of zolpidem were also evident in this study. As expected, zolpidem significantly (p<0.01) reduced the delta activity during non-REM sleep, and no significant change was observed in the delta activity of the sea breeze extract (KS-093) (see FIG. 5b). These results indicate that the sea bream extract (KS-093) increases NREM sleep without affecting physiological sleep.
이상적인 수면제는 입면시간이 빠르게 나타나고 수면유지가 지속되며 생리적인 수면에 변화를 주지 않는 특성을 가지는 약물이다[Sleep. 31, 259-270. Alexandre et al., 2008]. 따라서 본 발명의 갯방풍 추출물(KS-093)은 졸피뎀(zolpidem)과 같은 부작용이 있는 BDZ 및 non-BDZ 약물보다 더욱 안전하고 자연적인 수면을 증진시킬 수 있으리라 판단된다.An ideal sleeping agent is a drug that has fast awakening time, sustained sleep maintenance, and does not change physiological sleep [Sleep. 31, 259-270. Alexandre et al., 2008]. Therefore, it is believed that the sea bream extract (KS-093) of the present invention can promote natural sleep more safely than BDZ and non-BDZ drugs with side effects such as zolpidem.
시험예 3: 카페인 투여로 인한 수면장애 또는 불면증의 개선 효과Test Example 3: Improvement effect of sleep disorder or insomnia due to caffeine administration
시험예 3-1: 펜토바비탈 수면 유도 실험Test Example 3-1: Pentobarbital sleep induction experiment
펜토바비탈 수면유도 실험을 통해 카페인에 의해 유도된 마우스의 수면장애 또는 불면증에 대해 실시예의 갯방풍 추출물이 어떠한 효능을 나타내는지를 확인하고자 하였다. 이를 위하여, 마우스를 각 군당 10 마리씩 6그룹으로 나누어 준비하였다. 일반 대조군(Vehicle)은 5% tween 80-saline을 투여하였고, 카페인 50 mg/kg 단독 투여군, 카페인 50 mg/kg + 실시예 1의 갯방풍 추출물(KS-093) 100 mg/kg 투여군, 카페인 50 mg/kg + 실시예 1의 갯방풍 추출물(KS-093) 200 mg/kg 투여군, 카페인 50 mg/kg + 실시예 1의 갯방풍 추출물(KS-093) 300 mg/kg 투여군, 카페인 50 mg/kg + 졸피뎀(zolpidem) 10 mg/kg 투여군으로 나누었고, 각각의 농도로 경구투여(p.o.)한 후 펜토바비탈 (45 mg/kg, i.p.)에 의한 수면유도 효과를 분석하였다. 수면장애 마우스 모델을 위해 카페인을 음성대조군 약물로 사용하였다.Through the pentobarbital sleep induction experiment, it was intended to confirm the effect of the extracts of the examples on the sleep disorder or insomnia of mice induced by caffeine. To this end, mice were prepared by dividing into 6 groups of 10 mice in each group. The general control group (Vehicle) was administered with 5% tween 80-saline,
그 결과, 실시예 1의 갯방풍 추출물(KS-093)들은 카페인 투여로 인해 증가한 입면시간을 유의적으로 감소시켰고(도 6a 참조), 100 mg/kg, 200 mg/kg 및 300 mg/kg의 농도에서 유의적인 수면 지속 시간의 증가 효과를 나타내었다(도 6b 참조). 이는 본 발명의 갯방풍 추출물(KS-093)은 카페인 투여로 인한 수면장애 또는 불면증을 개선하는 효과가 있음을 나타낸다.As a result, the sea bream extract (KS-093) of Example 1 significantly reduced the elevation time increased due to caffeine administration (see FIG. 6a), and 100 mg/kg, 200 mg/kg and 300 mg/kg of concentration showed a significant increase in sleep duration (see Fig. 6b). This indicates that the sea bream extract (KS-093) of the present invention has an effect of improving sleep disorder or insomnia caused by caffeine administration.
시험예 3-2: 수면 구조 분석Test Example 3-2: Sleep structure analysis
실시예 1의 갯방풍 추출물(KS-093)의 수면의 양 및 질을 평가하기 위해 마우스의 수면 구조를 분석하고자 하였다. 이를 위하여, 마우스를 각 군당 7 마리씩 3그룹으로 나누어 준비하였다. 일반 대조군(Vehicle)은 5% tween 80-saline을 투여하였고, 카페인 25 mg/kg 단독 투여군, 실시예 1의 갯방풍 추출물(KS-093) 300 mg/kg + 카페인 25 mg/kg 투여군, ZPD(10 mg/kg) + 카페인 25 mg/kg 투여군으로 나누어 실험하였다.In order to evaluate the quantity and quality of sleep of the sea bream extract (KS-093) of Example 1, the sleep structure of mice was analyzed. To this end, the mice were prepared by dividing them into 3 groups of 7 mice per group. The general control group (Vehicle) was administered with 5% tween 80-saline, 25 mg/kg caffeine alone administration group, Example 1's seaweed extract (KS-093) 300 mg/kg +
도 7a의 hypnograms를 살펴보면, 카페인 단독 투여군은 일반대조군(Vehicle)에 비해 각성 시간(Wake)이 매우 길게 나타나고, 논렘수면(NREMS) 시간은 감소하였으나, 실시예 1의 갯방풍 추출물 투여군은 일반대조군과 비교하여 보았을 때 각성 시간(Wake) 및 논렘수면(NREMS) 시간의 큰 차이가 없다는 것을 알 수 있다. 좀 더 명확하게, 도 7b를 살펴보면, 카페인 단독 투여군은 일반대조군(Vehicle)에 비해 입면시간이 유의적(p<0.01)으로 크게 증가하고, 논렘수면 시간은 약 56% 감소하였다. 하지만, 카페인+갯방풍 추출물(KS-093) 투여군 및 카페인+졸피뎀(ZPD) 투여군은 입면 시간과 NREM 수면시간 모두 일반 대조군과 유사하게 나타났다. 이로 인해, 본 발명의 갯방풍 추출물(KS-093)은 카페인으로 인한 수면장애 또는 불면증을 개선하는 효과, 즉 카페인으로 인한 각성을 억제하는 효과가 있음을 알 수 있다.Looking at the hypnograms of Figure 7a, the caffeine alone administration group showed a very long wake time (Wake) compared to the general control group (Vehicle), and the non-REM sleep (NREMS) time decreased. When compared, it can be seen that there is no significant difference between wake time and non-REM sleep time (NREMS). More clearly, looking at Figure 7b, the caffeine alone administration group significantly increased the elevation time significantly (p <0.01) compared to the general control group (Vehicle), nonrem sleep time was reduced by about 56%. However, the caffeine + marigold extract (KS-093) administration group and the caffeine + zolpidem (ZPD) administration group showed similarity to the general control group in terms of both hypnotic time and NREM sleep time. From this, it can be seen that the sea bream extract (KS-093) of the present invention has an effect of improving sleep disorder or insomnia caused by caffeine, that is, an effect of suppressing arousal caused by caffeine.
하기에 본 발명의 갯방풍 추출물을 함유하는 조성물의 제제예를 설명하나, 본 발명은 이를 한정하고자 함이 아닌 단지 구체적으로 설명하고자 함이다.Hereinafter, formulation examples of compositions containing the extract of the present invention will be described, but the present invention is not intended to limit them, but only to be specifically described.
제제예 1. 산제의 제조Formulation Example 1. Preparation of powder
실시예 1에서 얻은 추출물 분말 300 mg300 mg of extract powder obtained in Example 1
유당 100 mg
탈크 10 mg
상기의 성분들을 혼합하고 기밀포에 충진하여 산제를 제조한다.A powder is prepared by mixing the above ingredients and filling them in an airtight bag.
제제예 2. 정제의 제조Formulation Example 2. Preparation of tablets
실시예 1에서 얻은 추출물 분말 300 mg 300 mg of extract powder obtained in Example 1
옥수수전분 100 mg
유당 100 mg
스테아린산 마그네슘 2 mg
상기의 성분들을 혼합한 후 통상의 정제의 제조방법에 따라서 타정하여 정제를 제조한다.After mixing the above ingredients, tablets are prepared by tableting according to a conventional tablet manufacturing method.
제제예 3. 캅셀제의 제조Formulation Example 3. Preparation of capsule formulation
실시예 1에서 얻은 추출물 분말 200 mg 200 mg of extract powder obtained in Example 1
결정성 셀룰로오스 3 mg3 mg of crystalline cellulose
락토오스 14.8 mgLactose 14.8 mg
스테아린산 마그네슘 0.2 mgMagnesium stearate 0.2 mg
통상의 캡슐제 제조방법에 따라 상기의 성분을 혼합하고 젤라틴 캡슐에 충전하여 캡슐제를 제조한다.Capsules are prepared by mixing the above ingredients and filling them into gelatin capsules according to a conventional capsule preparation method.
제제예 4. 주사제의 제조Formulation Example 4. Preparation of Injections
실시예 1에서 얻은 추출물 분말 600 mg600 mg of extract powder obtained in Example 1
만니톨 180 mgMannitol 180 mg
주사용 멸균 증류수 2974 mgSterile Distilled Water for Injection 2974 mg
Na2HPO4,12H2O 26 mgNa 2 HPO 4 , 12H 2 O 26 mg
통상의 주사제의 제조방법에 따라 1 앰플 당 상기의 성분 함량으로 제조한다. It is prepared with the above component content per 1 ampoule according to the conventional method for preparing injections.
제제예 5. 액제의 제조Formulation Example 5. Preparation of liquid formulation
실시예 1에서 얻은 추출물 분말 2 g2 g of extract powder obtained in Example 1
이성화당 10 gIsomerized sugar 10 g
만니톨 5 g5 g mannitol
정제수 적량Appropriate amount of purified water
통상의 액제의 제조방법에 따라 정제수에 각각의 성분을 가하여 용해시키고 레몬향을 적량 가한 다음 상기의 성분을 혼합한 다음 정제수를 가하여 전체를 정제수를 가하여 전체 100g으로 조절한 후 갈색병에 충진하여 멸균시켜 액제를 제조한다.According to the conventional liquid formulation manufacturing method, each component is dissolved in purified water, lemon flavor is added in an appropriate amount, the above components are mixed, and then purified water is added to adjust the total amount to 100g, and then filled into a brown bottle to be sterilized. to prepare a liquid.
제제예 6. 과립제의 제조Formulation Example 6. Preparation of granules
실시예 1에서 얻은 추출물 분말 1,000 mg1,000 mg of extract powder obtained in Example 1
비타민 혼합물 적량Appropriate amount of vitamin mixture
비타민 A 아세테이트 70 ㎍Vitamin A Acetate 70 μg
비타민 E 1.0 mgVitamin E 1.0 mg
비타민 B1 0.13 mgVitamin B1 0.13 mg
비타민 B2 0.15 mgVitamin B2 0.15 mg
비타민 B6 0.5 mgVitamin B6 0.5 mg
비타민 B12 0.2 ㎍Vitamin B12 0.2 μg
비타민 C 10 mg
비오틴 10 ㎍Biotin 10 μg
니코틴산아미드 1.7 mgNicotinamide 1.7 mg
엽산 50 ㎍
판토텐산 칼슘 0.5 mgCalcium Pantothenate 0.5 mg
무기질 혼합물 적량Appropriate amount of mineral mixture
황산제1철 1.75 mgFerrous sulfate 1.75 mg
산화아연 0.82 mgZinc Oxide 0.82 mg
탄산마그네슘 25.3 mgMagnesium Carbonate 25.3 mg
제1인산칼륨 15 mg
제2인산칼슘 55 mgDibasic Calcium Phosphate 55 mg
구연산칼륨 90 mg
탄산칼슘 100 mg
염화마그네슘 24.8 mgMagnesium Chloride 24.8 mg
상기의 비타민 및 미네랄 혼합물의 조성비는 비교적 과립제에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만, 그 배합비를 임의로 변형 실시하여도 무방하며, 통상의 과립제 제조방법에 따라 상기의 성분을 혼합한 다음, 과립을 제조하고, 통상의 방법에 따라 건강기능식품 조성물 제조에 사용할 수 있다. Although the composition ratio of the above vitamin and mineral mixture was prepared by mixing ingredients suitable for granules in a preferred embodiment, the mixing ratio may be arbitrarily modified, and after mixing the above ingredients according to a conventional granule manufacturing method, It can be prepared and used for preparing a health functional food composition according to a conventional method.
제제예 7. 기능성 음료의 제조Formulation Example 7. Manufacturing of functional beverages
실시예 1에서 얻은 추출물 분말 1,000 mg 1,000 mg of extract powder obtained in Example 1
구연산 1,000 mgCitric Acid 1,000 mg
올리고당 100 g100 g of oligosaccharides
매실농축액 2 g2 g plum concentrate
타우린 1 g1 g of taurine
정제수를 가하여 전체 900 mLAdd purified water to total 900 mL
통상의 건강음료 제조방법에 따라 상기의 성분을 혼합한 다음, 약 1 시간 동안 85 ℃에서 교반 가열한 후, 만들어진 용액을 여과하여 멸균된 2 L 용기에 취득하여 밀봉 멸균한 뒤 냉장 보관한 다음 본 발명의 기능성 음료 조성물 제조에 사용한다. After mixing the above ingredients according to the usual health drink manufacturing method, stirring and heating at 85 ° C. for about 1 hour, the resulting solution is filtered and collected in a sterilized 2 L container, sealed and sterilized, and then refrigerated. It is used for preparing the functional beverage composition of the present invention.
상기 조성비는 비교적 기호음료에 적합한 성분을 바람직한 실시예로 혼합 조성하였지만, 수요계층, 수요국가, 사용용도 등 지역적, 민족적 기호도에 따라서 그 배합비를 임의로 변형 실시하여도 무방하다.Although the composition ratio is a mixture of ingredients suitable for a relatively favorite beverage in a preferred embodiment, the mixing ratio may be arbitrarily modified according to regional and ethnic preferences such as the class of demand, the country of demand, and the purpose of use.
비록 본 발명이 상기에 언급된 바람직한 실시예로서 설명되었으나, 발명의 요지와 범위로부터 벗어남이 없이 다양한 수정이나 변형을 하는 것이 가능하다. 또한, 첨부된 청구범위는 본 발명의 요지에 속하는 이러한 수정이나 변형을 포함한다.Although the present invention has been described in terms of the preferred embodiments mentioned above, various modifications and variations are possible without departing from the spirit and scope of the invention. The appended claims also cover such modifications and variations as fall within the subject matter of this invention.
Claims (16)
상기 지상부는 갯방풍의 잎 및 줄기 중에서 선택되는 1종 이상이고,
상기 수면장애 또는 불면증은 입면시간이 증가되는 입면장애 및 논렘 수면시간이 감소되는 숙면장애 중에서 선택되는 1종 이상인 것을 특징으로 하는 수면장애 또는 불면증의 예방 또는 개선용 식품 조성물.A food composition for preventing or improving sleep disorder or insomnia using Glehnia littorali s aboveground extract as an active ingredient,
The aerial part is at least one selected from the leaves and stems of the sea breeze,
The sleep disorder or insomnia is a food composition for preventing or improving sleep disorders or insomnia, characterized in that at least one selected from the sleeping disorder that increases the sleeping time and sleep disorder that reduces nonrem sleep time.
상기 갯방풍 지상부 추출물은 물, 탄소수 1 내지 4의 저급알코올 또는 이들의 혼합용매로 추출된 것을 특징으로 하는 수면장애 또는 불면증의 예방 또는 개선용 식품 조성물.According to claim 1,
The aerial part extract of the sea bream is a food composition for preventing or improving sleep disorders or insomnia, characterized in that extracted with water, lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof.
상기 혼합용매는 20 내지 80 부피%의 메탄올, 에탄올, 부탄올 또는 프로판올 수용액인 것을 특징으로 하는 수면장애 또는 불면증의 예방 또는 개선용 식품 조성물.According to claim 2,
The mixed solvent is a food composition for preventing or improving sleep disorders or insomnia, characterized in that 20 to 80% by volume of methanol, ethanol, butanol or propanol aqueous solution.
상기 혼합용매는 60 내지 80 부피%의 에탄올 수용액인 것을 특징으로 하는 수면장애 또는 불면증의 예방 또는 개선용 식품 조성물.According to claim 2,
The mixed solvent is a food composition for preventing or improving sleep disorders or insomnia, characterized in that 60 to 80% by volume of ethanol aqueous solution.
상기 갯방풍 지상부 추출물은 65 내지 95 ℃의 추출온도로 추출된 것을 특징으로 하는 수면장애 또는 불면증의 예방 또는 개선용 식품 조성물.According to claim 1,
The extract of the aerial part of the sea breeze is a food composition for preventing or improving sleep disorders or insomnia, characterized in that extracted at an extraction temperature of 65 to 95 ℃.
상기 식품 조성물의 제형은 분말, 과립, 정제, 캡슐 또는 음료인 것을 특징으로 하는 수면장애 또는 불면증의 예방 또는 개선용 식품 조성물.According to claim 1,
The formulation of the food composition is a food composition for preventing or improving sleep disorders or insomnia, characterized in that the powder, granules, tablets, capsules or drinks.
상기 지상부는 갯방풍의 잎 및 줄기 중에서 선택되는 1종 이상이고,
상기 수면장애 또는 불면증은 입면시간이 증가되는 입면장애 및 논렘 수면시간이 감소되는 숙면장애 중에서 선택되는 1종 이상인 것을 특징으로 하는 수면장애 또는 불면증의 예방 또는 치료용 약학 조성물.A pharmaceutical composition for the prevention or treatment of sleep disorder or insomnia, comprising an extract of the aerial part of Glehnia littorali s as an active ingredient,
The aerial part is at least one selected from the leaves and stems of the sea breeze,
The sleep disorder or insomnia is a pharmaceutical composition for preventing or treating sleep disorders or insomnia, characterized in that at least one selected from the sleeping disorder that increases the sleeping time and sleep disorder that reduces the non-REM sleep time.
상기 갯방풍 지상부 추출물은 물, 탄소수 1 내지 4의 저급알코올 또는 이들의 혼합용매로 추출된 것을 특징으로 하는 수면장애 또는 불면증의 예방 또는 치료용 약학 조성물. According to claim 10,
The extract of the aerial part of the sea bream is a pharmaceutical composition for preventing or treating sleep disorders or insomnia, characterized in that extracted with water, lower alcohol having 1 to 4 carbon atoms or a mixed solvent thereof.
상기 혼합용매는 20 내지 80 부피%의 메탄올, 에탄올, 부탄올 또는 프로판올 수용액인 것을 특징으로 하는 수면장애 또는 불면증의 예방 또는 치료용 약학 조성물. According to claim 11,
The mixed solvent is a pharmaceutical composition for preventing or treating sleep disorders or insomnia, characterized in that 20 to 80% by volume of methanol, ethanol, butanol or propanol aqueous solution.
상기 혼합용매는 60 내지 80 부피%의 에탄올 수용액인 것을 특징으로 하는 수면장애 또는 불면증의 예방 또는 치료용 약학 조성물. According to claim 11,
The mixed solvent is a pharmaceutical composition for preventing or treating sleep disorders or insomnia, characterized in that 60 to 80% by volume of ethanol aqueous solution.
상기 갯방풍 지상부 추출물은 65 내지 95 ℃의 추출온도로 추출된 것을 특징으로 하는 수면장애 또는 불면증의 예방 또는 치료용 약학 조성물. According to claim 10,
The pharmaceutical composition for preventing or treating sleep disorder or insomnia, characterized in that the extract of the aerial part of the sea bream is extracted at an extraction temperature of 65 to 95 ° C.
상기 지상부는 갯방풍의 잎 및 줄기 중에서 선택되는 1종 이상이고,
상기 수면장애 또는 불면증은 입면시간이 증가되는 입면장애 및 논렘 수면시간이 감소되는 숙면장애 중에서 선택되는 1종 이상인 것을 특징으로 하는 수면장애 또는 불면증의 예방 또는 개선용 건강기능식품 조성물.A health functional food composition for preventing or improving sleep disorder or insomnia using Glehnia littorali s aerial part extract as an active ingredient,
The aerial part is at least one selected from the leaves and stems of the sea breeze,
The sleep disorder or insomnia is a health functional food composition for preventing or improving sleep disorder or insomnia, characterized in that at least one selected from the sleep disorder in which the sleeping time is increased and the sleep disorder in which the non-REM sleep time is reduced.
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