KR102513550B1 - Natural immersion liquid for improving storability of peeled bellflower root and method for improving storability of peeled bellflower root using the same - Google Patents

Natural immersion liquid for improving storability of peeled bellflower root and method for improving storability of peeled bellflower root using the same Download PDF

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KR102513550B1
KR102513550B1 KR1020220117630A KR20220117630A KR102513550B1 KR 102513550 B1 KR102513550 B1 KR 102513550B1 KR 1020220117630 A KR1020220117630 A KR 1020220117630A KR 20220117630 A KR20220117630 A KR 20220117630A KR 102513550 B1 KR102513550 B1 KR 102513550B1
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peeled
bellflower
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허명제
주광식
권성희
박병규
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인천광역시
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23BPRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
    • A23B7/00Preservation or chemical ripening of fruit or vegetables
    • A23B7/14Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
    • A23B7/153Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463Organic compounds; Microorganisms; Enzymes
    • A23L3/3472Compounds of undetermined constitution obtained from animals or plants
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3589Apparatus for preserving using liquids
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23PSHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P20/00Coating of foodstuffs; Coatings therefor; Making laminated, multi-layered, stuffed or hollow foodstuffs
    • A23P20/10Coating with edible coatings, e.g. with oils or fats
    • A23P20/15Apparatus or processes for coating with liquid or semi-liquid products
    • A23P20/17Apparatus or processes for coating with liquid or semi-liquid products by dipping in a bath
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts

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Abstract

The present invention relates to a technique for improving the storability of peeled bellflower root inhibiting microbial growth and preventing browning. The present invention includes: washing, peeling, and cutting bellflower root; immersing the peeled bellflower root in a natural immersion liquid; and packaging the same. More particularly, the present invention relates to a natural immersion liquid for improving the storability of peeled bellflower root, which comprises, as active ingredients, Artemisia princeps Pampanini extract, salicornia extract, wilfordi root extract, and grapefruit seed extract. According to the present invention, a method for improving the storability of peeled bellflower root can maintain the unique aroma and taste of bellflower root and maintain the white surface to inhibit the growth of microorganisms while maintaining high marketability, to store the same for a long time and extend the expiration date, thereby providing the bellflower root that consumers prefer.

Description

박피 도라지의 저장성 향상을 위한 천연침지액 및 이를 이용한 박피 도라지의 저장성 향상 방법{Natural immersion liquid for improving storability of peeled bellflower root and method for improving storability of peeled bellflower root using the same}Natural immersion liquid for improving storability of peeled bellflower root and method for improving storability of peeled bellflower root using the same}

본 발명은 천연 추출물을 함유하는 박피 도라지의 저장성을 향상시키는 천연침지액에 관한 것으로서, 보다 구체적으로는 강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물 및 자몽종자 추출물을 함유하며 미생물 성장억제 및 갈변방지 효과가 있는 박피 도라지의 저장성 향상을 위한 천연침지액에 관한 것이다.The present invention relates to a natural soaking liquid for improving the storability of peeled bellflowers containing natural extracts, and more specifically, to a natural soaking liquid containing Ganghwa lion's foot mugwort extract, glasswort extract, white hazelnut extract, and grapefruit seed extract, which inhibits microbial growth and prevents browning. It relates to a natural immersion liquid for improving the storage stability of peeled bellflowers, which is effective.

건강에 대한 관심이 증가되고 신선식품에 대한 소비자의 선호도와 기호도가 증가하면서 신선한 과일과 채소류의 소비는 꾸준히 늘어나고 있다.Consumption of fresh fruits and vegetables is steadily increasing as interest in health increases and consumer preference and preference for fresh food increases.

최근에는 신선도와 편의성을 동시에 추구하는 소비자의 요구를 충족시켜 줄 수 있는 최소가공 채소류(minimally processed vegetables)의 소비가 급격하게 증가하고 있다.Recently, the consumption of minimally processed vegetables that can meet the needs of consumers who pursue freshness and convenience at the same time is rapidly increasing.

이러한 최소가공 채소류는 생산 단계에서 세척, 박피, 절단 등의 과정을 거치기 때문에 소비자는 이러한 제품을 구입한 후에 전처리 과정을 거치지 않고 바로 사용할 수 있는 장점이 있다.Since these minimally processed vegetables go through processes such as washing, peeling, and cutting in the production stage, consumers have the advantage of using these products immediately without going through a pretreatment process after purchasing them.

일반적으로 근채류(root vegetables)란 뿌리를 식용으로 하는 채소를 말하며 당근, 더덕, 도라지, 마늘, 무, 연근, 우엉 등이 포함된다.In general, root vegetables refer to vegetables whose roots are edible, and include carrots, deodeok, bellflower, garlic, radish, lotus root, burdock, and the like.

최근 근채류에 대한 긍정적인 영양학적 연구 결과가 알려지며 근채류의 소비는 꾸준히 늘어나고 있다.Recently, positive nutritional research results on root vegetables have been known, and the consumption of root vegetables is steadily increasing.

신선편이 근채류 제품은 근채류를 세척, 박피, 절단한 후 소비자의 요구에 맞는 형태와 크기로 포장하여 유통, 판매하는 형태를 말하며 이용의 편의성을 장점으로 한다.Fresh cut root vegetable products refer to a form of washing, peeling, and cutting root vegetables, packaging them in a shape and size that meets the needs of consumers, and distributing and selling them, with the advantage of convenience of use.

그러나, 박피, 절단 등의 가공공정 중 발생한 조직의 손상에 따른 연화, 절단면이 공기에 노출됨에 따른 갈변, 미생물 오염 및 증식 등의 문제가 발생하여 저장성 및 안전성이 떨어지는 문제점이 있다.However, problems such as softening due to damage to tissues generated during processing processes such as peeling and cutting, browning due to exposure of the cut surface to air, and contamination and proliferation of microorganisms occur, resulting in poor storage and safety.

갈변현상(browing reaction)은 신선편이 채소류의 가공 또는 저장 중 고유한 색깔이 변화를 일으켜 갈색 또는 암갈색으로 변하는 것을 말하여 채소류의 외관과 풍미를 손상, 소비자의 기호성 저하, 영양가 손실 등에 영향을 미쳐 상품의 가치를 직/간접적으로 떨어뜨리기 때문에 채소류의 보관과 저장, 가공 등에 있어 매우 중요하여 냉장, 포장 등의 방법으로 갈변반응을 억제하는 것이 필수적이다.Browning reaction refers to a change in the unique color of fresh cut vegetables to brown or dark brown during processing or storage. Because it directly or indirectly lowers the value of vegetables, it is very important in storage, storage, and processing of vegetables, so it is essential to suppress the browning reaction by methods such as refrigeration and packaging.

일반적으로 갈변과 미생물 증식을 억제하기 위해 사용하는 화학물질은 소비자에게 거부감을 주거나 건강상의 문제가 발생할 수 있어 천연물질을 사용한 미생물 성장 및 갈변억제에 대한 소비자의 요구가 증가하고 있는 추세이다.In general, chemicals used to inhibit browning and microbial growth may give consumers a sense of rejection or cause health problems, so consumer demand for microbial growth and browning inhibition using natural materials is increasing.

한편, 도라지 뿌리는 전통적으로 도라지 무침, 도라지 초무침, 도라지 볶음, 도라지 산적, 도라지장아찌, 도라지 밥 등과 같은 식품으로써 널리 이용되고 있다.On the other hand, bellflower roots are traditionally widely used as foods such as bellflower seasoning, bellflower vinegar, bellflower stir-fry, bellflower bandits, bellflower pickles, bellflower rice, and the like.

그러나, 식용 목적의 도라지는 장기간 보관이 곤란하여 채취한 후 바로 섭식하여야 한다는 문제점이 있다.However, there is a problem that it is difficult to store bellflower for long-term storage for edible purposes and must be eaten immediately after harvesting.

특히, 바쁜 현대인이 가정에서 도라지를 다듬는 일은 별로 없고 상업적으로 도라지를 박피하여 세정하고 가늘게 쪼갠 상태로 포장 판매되고 있으므로 유통 중 미생물 성장에 의한 변질 및 부패, 갈변현상에 의한 상품성 저하 등의 우려가 매우 크다고 할 수 있다.In particular, busy modern people rarely trim bellflowers at home, and commercial bellflowers are peeled, cleaned, and sliced and sold in packaging, so there are very concerns about deterioration and spoilage due to microbial growth during distribution, and deterioration in marketability due to browning. can be said to be large.

도라지는 뿌리껍질이 붙어 있는 상태에서는 저장성이 상대적으로 양호한 편이지만 식재료로 사용하기 위해서 뿌리껍질을 제거하면 저장성이 현저히 떨어지고 갈변되며 수분, 온도, 광선, 산소, 뿌리내 효소 및 미생물 작용에 의해 부패될 수 있다.Bellflower has relatively good storability when the root bark is attached, but when the root bark is removed for use as a food material, the storage quality is significantly reduced, and it turns brown and can be corrupted by moisture, temperature, light, oxygen, enzymes in the root, and microbial action. can

현실적으로도 박피 도라지의 유통기한은 하루 정도에 불과하므로 유통기한을 연장하기 위해 진공포장, 탈산소제 포장, 액체침지 포장 등의 형태로 유통되고 있다.Realistically, the shelf life of peeled bellflower is only about a day, so it is distributed in the form of vacuum packaging, oxygen scavenger packaging, liquid immersion packaging, etc. to extend the shelf life.

액체침지 포장 유통의 경우, 미생물의 성장을 억제하고 갈변을 방지하기 위해 합성보존료(소르빈산칼륨), 산화방지제(이산화황) 등 화학물질을 사용하여 소비자들이 기피하거나 건강상의 문제가 발생할 가능성이 있다.In the case of liquid immersion packaging distribution, chemicals such as synthetic preservatives (potassium sorbate) and antioxidants (sulfur dioxide) are used to inhibit the growth of microorganisms and prevent browning, which may cause health problems or avoid consumers.

또한, 일부에서는 박피 도라지의 유통기한을 연장하기 위해 출혈성 위장염과 소화 장애를 유발하는 것으로 알려진 공업용 활산알루미늄 희석액으로 세척하는 불법행위가 적발되는 사례도 있었다.In addition, in some cases, an illegal act of washing with an industrial aluminum activated acid diluted solution known to cause hemorrhagic gastroenteritis and digestive disorders was discovered in order to extend the shelf life of peeled bellflowers.

대한민국 공개특허 제10-2015-0034327호Republic of Korea Patent Publication No. 10-2015-0034327 대한민국 공개특허 제10-2016-0084050호Republic of Korea Patent Publication No. 10-2016-0084050 대한민국 등록특허 제10-1803756호Republic of Korea Patent No. 10-1803756 대한민국 등록특허 제10-0915016호Republic of Korea Patent No. 10-0915016

따라서, 본 발명은 이러한 문제점들을 해결하기 위한 것으로서, 본 발명의 목적은 도라지 특유의 향과 맛을 유지하면서 박피 도라지의 상품성을 높이기 위해 천연 추출물을 유효성분으로 함유하는 천연침지액을 제공하여 미생물 성장을 억제하여 저장성을 향상시키는 방법을 제공하기 위한 것이다.Therefore, the present invention is to solve these problems, and an object of the present invention is to provide a natural soaking liquid containing natural extracts as active ingredients in order to increase the marketability of peeled bellflower while maintaining the unique aroma and taste of bellflower, thereby growing microorganisms. It is to provide a method for improving storage stability by suppressing.

또한, 본 발명의 목적은 박피 도라지의 갈변 현상을 효과적으로 방지하여 상품성 및 유통성 향상 방법을 제공하기 위한 것이다.In addition, an object of the present invention is to provide a method for improving marketability and distribution by effectively preventing browning of peeled bellflower.

또한, 본 발명의 목적은 상기한 첫 번째 및 두 번째 목적에 따른 방법에 의하여 얻어지는 저장성, 상품성 및 유통성이 향상된 박피 도라지를 제공하기 위한 것이다.In addition, an object of the present invention is to provide peeled bellflower with improved storage, marketability and distribution obtained by the method according to the first and second objects described above.

이와 같은 기술적 과제를 해결하기 위해 본 발명은, 상기 목적을 달성하기 위해서 본 발명은 강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물 및 자몽종자 추출물을 유효성분으로 함유하는 저장성 향상을 위한 천연침지액을 특징으로 한다.In order to solve such a technical problem, the present invention, in order to achieve the above object, the present invention is a natural soaking liquid for improving storage stability containing Ganghwasajabalyak mugwort extract, seaweed extract, white hazelnut extract and grapefruit seed extract as active ingredients. to be characterized

상기 강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물 및 자몽종자 추출물을 중량비 기준으로 총 3.02중량%가 정제수 96.98중량%에 포함되는 것을 특징으로 한다.It is characterized in that a total of 3.02% by weight of the Ganghwa lion's foot wormwood extract, glasswort extract, baekhasuo extract and grapefruit seed extract is included in 96.98% by weight of purified water.

본 발명은 강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물 및 자몽종자 추출물을 유효성분으로 함유하여 미생물 성장억제 및 갈변현상을 방지하는 근채류(박피 도라지)의 저장성 향상을 위한 천연침지액을 제공한다.The present invention provides a natural soaking liquid for improving storage stability of root vegetables (peeled bellflower), which inhibits microbial growth and prevents browning by containing Ganghwasaja's foot wormwood extract, glasswort extract, white hazelnut extract, and grapefruit seed extract as active ingredients.

본 발명의 강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물 및 자몽종자 추출물은 미생물 항균활성 효과가 우수하여 미생물의 성장을 억제하고 효소적 갈변현상을 효과적으로 방지하여 준다.The Ganghwa lion's foot wormwood extract, glasswort extract, white hazelnut extract, and grapefruit seed extract have excellent antibacterial activity against microorganisms, thereby inhibiting the growth of microorganisms and effectively preventing enzymatic browning.

본 발명에 따른 상기 강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물 및 자몽종자 추출물 혼합 침지액에 박피 도라지를 넣어 침지 포장함으로써 미생물의 성장을 억제하고 갈변현상을 방지하여 도라지 고유의 맛과 향이 그대로 유지되고 저장성 및 유통기한을 연장시켜 준다.By putting the peeled bellflower in the mixed immersion liquid of the Ganghwa lion's foot medicinal mugwort extract, glasswort extract, white hazelnut extract, and grapefruit seed extract according to the present invention, immersion packaging suppresses the growth of microorganisms and prevents browning, thereby maintaining the unique taste and aroma of bellflower. and prolongs shelf life and shelf life.

본 발명에 따르면, 천연침지액은 폴리페놀, 플라보노이드 등의 성분을 함유하는 추출물의 효과로 인하여 박피 도라지의 유통 중 미생물의 성장 및 갈변현상을 최대한 억제할 수 있어 소비자들이 선호하는 도라지를 제공할 수 있는 효과가 있다.According to the present invention, the natural immersion liquid can suppress the growth of microorganisms and browning as much as possible during distribution of peeled bellflowers due to the effect of extracts containing components such as polyphenols and flavonoids, thereby providing bellflowers preferred by consumers. There is an effect.

또한, 박피 도라지의 영양소 파괴도 최소화시켜 주며 냉장온도에서 장시간 미생물의 성장 및 갈변현상이 억제되어 박피 도라지의 유통기간을 효과적으로 연장시킬 수 있는 효과가 있다.In addition, it minimizes the destruction of nutrients in the peeled bellflower, and inhibits the growth and browning of microorganisms for a long time at refrigeration temperature, thereby effectively extending the shelf life of the peeled bellflower.

도 1은 본 발명에 따른 박피 도라지의 저장성 향상을 위한 천연침지액을 이용하여 미생물 성장 억제 및 갈변이 방지된 박피 도라지를 제조하는 순서도이다.
도 2는 본 발명에 따른 박피 도라지의 저장성 향상을 위한 천연침지액에 박피도라지를 10분간 침지하고 꺼내어 자연 탈수시킨 후 냉장온도(4℃)에 보관하면서 시간에 따른 박피 도라지의 외관 변화를 나타내는 도면이다.
도 3은 본 발명에 따른 박피 도라지의 저장성 향상을 위한 천연침지액에 보관하면서 측정한 미생물수(일반세균수, 대장균군수, 진균수)의 변화를 나타내는 도면이다.
1 is a flow chart for producing peeled bellflowers in which microbial growth is inhibited and browning is prevented using a natural immersion liquid for improving the storage stability of peeled bellflowers according to the present invention.
Figure 2 is a view showing the appearance change of peeled bellflower over time while storing at refrigeration temperature (4 ℃) after immersing peeled bellflower in natural immersion liquid for 10 minutes to improve storage stability of peeled bellflower according to the present invention, taking it out, and naturally dehydrating it. am.
Figure 3 is a view showing the change in the number of microorganisms (general bacterial count, coliform count, fungal count) measured while stored in a natural immersion liquid for improving the storage stability of peeled bellflowers according to the present invention.

본 발명은 다양한 변경을 가할 수 있고 여러 가지 실시예를 가질 수 있다. 특정 실시 예를 도면에 예시하여 본 발명을 실시하기 위한 구체적인 내용에 대해서 보다 상세히 설명하고자 한다.The present invention can make various changes and have various embodiments. By exemplifying specific embodiments in the drawings, the specific contents for carrying out the present invention will be described in more detail.

그러나, 이들 실시예는 단지 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 범위가 이들 실시예로 한정되지 않음을 알린다.However, it is noted that these examples are only for explaining the present invention more specifically, and the scope of the present invention is not limited to these examples.

이하에서는 본 발명의 바람직한 제조방법을 보다 상세히 설명한다.Hereinafter, a preferred manufacturing method of the present invention will be described in more detail.

본 발명은 천연침지액 조성물 총 중량에 대해 1.0중량% 강화사자발약쑥 추출물, 1.0중량% 함초 추출물, 1.0중량% 백하수오 추출물 및 0.02중량% 자몽종자 추출물을 유효성분으로 포함함으로써 상기 천연침지액은 세척 후 박피 및 절단한 근채류에 적용하는 경우 효과적으로 미생물 성장 및 갈변현상을 방지 할 수 있는 저장성 향상을 위한 천연침지액을 제공한다.The present invention contains, as active ingredients, 1.0% by weight of strengthened lion's foot mugwort extract, 1.0% by weight of glasswort extract, 1.0% by weight of white hazelnut extract, and 0.02% by weight of grapefruit seed extract, based on the total weight of the natural immersion liquid composition, so that the natural immersion liquid is washed. When applied to post-peeled and cut root vegetables, it provides a natural soaking liquid for improving storage stability that can effectively prevent microbial growth and browning.

강화사자발약쑥(Artemisia princeps Pampanini)은 국화과에 속하는 초본식물로 인천 강화도 지역에서 주로 자생하며 키는 70cm 내외로 줄기가 다소 굵고 곧게 자라며 연보라색의 꽃이 핀다.Ganghwa lion-footed mugwort (Artemisia princeps Pampanini) is a herbaceous plant belonging to the Asteraceae family. It grows wild mainly in Ganghwa-do, Incheon. Its height is around 70cm, its stem grows rather thick and straight, and its light purple flowers bloom.

특히, 잎이 넓고 끝이 보족하며 짙은 녹색으로 사자발 모양으로 갈려져 있고 특유의 향을 가지고 있다. 강화사자발약쑥은 황해쑥, 인진쑥에 비해 폴리페놀과 플라보노이드 함량이 높고 항균, 항산화 및 항염증 효과가 우수하다고 알려져 있다.In particular, the leaves are wide and the ends are thick, dark green, ground in the shape of a lion's foot, and have a unique scent. Ganghwa Sajabal Yak Wormwood is known to have higher polyphenol and flavonoid content than Hwanghae Wormwood and Injin Wormwood, and has excellent antibacterial, antioxidant and anti-inflammatory effects.

함초(Salicornia herbacea)는 염전주변이나 갯벌에서 자라는 염생식물로 명 아주과(Chenopdiaceae)에 속하며 마디가 퉁퉁하게 튀어나온 풀이라 하여 퉁퉁마디라고도 한다. 갯벌식물인 함초는 다량의 염분을 체내에 축적하고 있을 뿐만 아니라 천연 미네랄이 풍부하고 필수지방산인 리놀렌산과 필수 아미노산인 발린, 류신, 히스티딘 등을 다량 함유하는 식물로 식품소재로 활용되고 있다. 이외에 플라보노이드 성분도 함유하고 있어 항산화 효과가 있는 것으로 알려져 있다.Hamcho (Salicornia herbacea) is a salt plant that grows around salterns or on tidal flats, and belongs to the Chenopdiaceae family. Hamcho, a tidal flat plant, not only accumulates a large amount of salt in the body, but is also rich in natural minerals and is used as a food ingredient as a plant containing large amounts of essential fatty acids such as linolenic acid and essential amino acids such as valine, leucine, and histidine. In addition, it is known to have an antioxidant effect because it contains flavonoids.

백하수오(Cynanchi wilfordii Radix)는 덩이뿌리로 원불 모양이고 길이는 5-10cm, 지름 15-35mm로 알려져 있으며 황갈색을 띤 표면에 오목하게 함몰된 가로로 긴 피공 및 뿌리 흔적이 있다.White hazelnut (Cynanchi wilfordii Radix) is a tuberous root, round, 5-10 cm long, 15-35 mm in diameter, and is known to have concave, concave, horizontally long pores and root traces on the yellowish brown surface.

우리나라에서는 전국 각지에서 야생하고 있으며 맛은 단맛, 쓴맛 그리고 떫은맛을 가지고 있어 주로 찌거나 끓인 후 건조하여 사용해 왔다.In Korea, it is wild all over the country and has a sweet, bitter, and astringent taste, so it has been used mainly after being steamed or boiled and then dried.

백하수오의 성분은 acetophenone과 polyoxypregnane glycoside가 주요성분이라는 보고가 있으며 항균 및 항산화 효과가 있는 것으로 알려져 있다. 자몽종자추출물(Grapefruit seed extract)은 운향과 자몽의 종자를 물, 에탄올 또는 글리세린으로 추출하여 얻어지는 것으로 지방산, 플라보노이드 등이 주성분이며 ascorbic acid, sterols, palmitic acid, naringin 등을 함유하고 있다. 자몽종자추출물은 식품첨가물공전에 천연첨가물로 등재되어 사용량 및 사용대상 식품의 제한이 없으며 미생물의 항균효과가 우수한 것으로 알려져 있다.It has been reported that acetophenone and polyoxypregnane glycoside are the main components of white hazelnut, and it is known to have antibacterial and antioxidant effects. Grapefruit seed extract is obtained by extracting rue and grapefruit seeds with water, ethanol or glycerin. Its main components are fatty acids and flavonoids, and it contains ascorbic acid, sterols, palmitic acid, and naringin. Grapefruit seed extract is listed as a natural additive in the Food Additives Codex, so there are no restrictions on usage and target foods, and it is known to have excellent antibacterial effects against microorganisms.

도 1은 본 발명의 저장성 향상 천연침지액을 이용하여 미생물 성장억제 및 갈변현상이 방지된 박피 도라지를 제조하는 순서도이다.1 is a flow chart for producing peeled bellflowers in which microbial growth is inhibited and browning is prevented using the natural immersion liquid for improving storage stability of the present invention.

도시된 바와 같이 도라지를 선별하여 세척, 박피한 후 절단하여 도라지 절편을 제조하는 단계; 강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물 및 자몽종자 추출물을 포함하여 저장성 향상을 위한 천연침지액을 제조하는 단계; 상기 박피 절단한 도라지를 상기 제조된 저장성 향상을 위한 천연침지액로 처리하는 단계; 냉장(4℃)에서 저장 및 보관하는 단계;를 포함하여 미생물 성장억제 및 갈변을 방지하는 박피 도라지의 저장성 향상 방법을 제공한다.As shown, preparing bellflower slices by selecting bellflower, washing, peeling, and then cutting; Preparing a natural soaking liquid for improving storage stability including Ganghwasajabalak mugwort extract, glasswort extract, baekhasuo extract, and grapefruit seed extract; Treating the peeled and cut bellflower with a natural soaking liquid for improving storage stability; Storing and storing at refrigeration (4 ° C.); provides a method for improving the shelf life of peeled bellflower, including inhibiting microbial growth and preventing browning.

본 저장성 향상을 위한 천연침지액은 미생물 성장 및 갈변현상이 일어나는 모든 과일, 채소류에 적용할 수 있으며 바람직하게는 도라지, 더덕, 연근 등에 적용할 수 있다.This natural immersion liquid for improving storage stability can be applied to all fruits and vegetables in which microbial growth and browning occur, and can be preferably applied to bellflower, deodeok, and lotus root.

이하, 본 발명의 저장성 향상을 위한 천연침지액 및 이를 이용한 박피 도라지의 저장성 향상(미생물 성장 억제 및 갈변 방지 방법)에 대해 비교예, 실시예, 실험예를 들어 상세히 설명하기로 한다.Hereinafter, the natural immersion liquid for improving storage stability of the present invention and the improvement of storage stability of peeled bellflowers using the same (method for inhibiting microbial growth and preventing browning) will be described in detail with comparative examples, examples, and experimental examples.

비교예comparative example

비교예 1Comparative Example 1

강화사자발약쑥 분말 시료 10g에 50% 에탄올 용액 90g을 넣고 50℃ 항온진탕조에서 24시간 동안 추출하였다. 추출액을 원심분리(3,000 x g, 10분)한 후 상등액을 취하여 0.2㎛ 필터로 여과하여 추출물을 수득하였다.90 g of a 50% ethanol solution was added to 10 g of powdered mugwort samples and extracted for 24 hours in a 50° C. constant temperature shaking bath. After the extract was centrifuged (3,000 x g, 10 minutes), the supernatant was taken and filtered through a 0.2 μm filter to obtain an extract.

상기 수득된 강화사자발약쑥 추출물 1.0g을 취하여 정제수 99.0g에 넣고 잘 혼합 한 후 미생물 성장 억제 및 갈변 현상을 방지하는 저장성 향상을 위한 천연침지액을 제조하였다.After taking 1.0 g of the obtained Ganghwasajabal wormwood extract, adding it to 99.0 g of purified water and mixing well, a natural soaking liquid for improving storage stability to inhibit microbial growth and prevent browning was prepared.

준비한 박피 도라지를 상기 저장상 향상을 위한 천연침지액에 10분간 침지하고 꺼내어 자연탈수 시킨 후 물기를 제거한 박피 도라지를 냉장(4℃)에서 12일간 보관하면서 외관의 변화를 관찰하였다.The prepared peeled bellflower was immersed in the natural immersion liquid for improving the storage phase for 10 minutes, taken out and naturally dehydrated, and then the dried bellflower was stored in a refrigerator (4 ° C) for 12 days to observe changes in appearance.

또한, 박피 도라지를 상기 저장성 향상을 위한 천연침지액에 넣어 포장한 후 냉장(4℃)에서 12일간 보관하면서 미생물(일반세균수, 대장균군, 진균수)의 변화를 측정하였다.In addition, after packing the peeled bellflower seeds in the natural soaking liquid for improving storage stability, the change in microorganisms (general bacterial count, coliform group, fungal count) was measured while stored in a refrigerator (4 ° C) for 12 days.

비교예 2Comparative Example 2

함초 분말 시료 10g에 70% 에탄올 용액 90g을 넣고 50℃ 항온진탕조에서 24시간동안 추출하였다. 추출액을 원심분리(3,000 x g, 10분)한 후 상등액을 취하여 0.2㎛필터로 여과하여 추출물을 수득하였다.90 g of 70% ethanol solution was added to 10 g of glasswort powder sample and extracted for 24 hours in a 50 ° C constant temperature shaking bath. After the extract was centrifuged (3,000 x g, 10 minutes), the supernatant was taken and filtered through a 0.2 μm filter to obtain an extract.

저장성 향상을 위한 천연침지액의 제조시, 상기 수득된 함초 추출물 1g과 정제수 99.0g 포함하는 것을 제외하고는 비교예 1과 동일한 방법으로 박피 도라지를 처리하였다.When preparing a natural soaking liquid for improving storage stability, peeled bellflower seeds were treated in the same manner as in Comparative Example 1, except that 1 g of the obtained seaweed extract and 99.0 g of purified water were included.

비교예 3Comparative Example 3

백하수오 분말 시료 10g에 70% 에탄올 용액 90g을 넣고 50℃ 항온진탕조에서 24시간 동안 추출하였다. 추출액을 원심분리(3,000 x g, 10분)한 후 상등액을 취하여 0.2㎛ 필터로 여과하여 추출물을 수득하였다.90 g of a 70% ethanol solution was added to 10 g of a white hazelnut powder sample, and extraction was performed in a 50° C. constant temperature shaking bath for 24 hours. After the extract was centrifuged (3,000 x g, 10 minutes), the supernatant was taken and filtered through a 0.2 μm filter to obtain an extract.

저장성 향상을 위한 천연침지액의 제조시, 상기 수득된 백하수오 추출물 1g과 정제수 99.0g 포함하는 것을 제외하고는 비교예 1과 동일한 방법으로 박피 도라지를 처리하였다.In the preparation of the natural soaking liquid for improving storage stability, peeled bellflower seeds were treated in the same manner as in Comparative Example 1, except that 1 g of the obtained white hazelnut extract and 99.0 g of purified water were included.

비교예 4Comparative Example 4

자몽종자추출물은 액체타입의 제품(DF-100)을 활용하였다. 저장성 향상을 위한 천연침지액의 제조시, 자몽종자추출물 0.02g과 정제수 99.08g 포함하는 것을 제외하고는 비교예 1과 동일한 방법으로 박피 도라지를 처리하였다.For the grapefruit seed extract, a liquid type product (DF-100) was used. When preparing a natural soaking solution for improving storage stability, peeled bellflower seeds were treated in the same manner as in Comparative Example 1, except that 0.02 g of grapefruit seed extract and 99.08 g of purified water were included.

실시예Example

실시예 1Example 1

저장성 향상을 위한 천연침지액의 제조시, 강화사자발약쑥 추출물 1g, 함초추출물 1g, 백하수오 추출물 1g과 정제수 97g을 포함하는 것을 제외하고 비교예 1과 동일한 방법으로 박피 도라지를 처리하였다.When preparing a natural soaking solution for improving storage stability, peeled bellflower seeds were treated in the same manner as in Comparative Example 1, except that 1 g of Ganghwasajabal medicinal mugwort extract, 1 g of glasswort extract, 1 g of white hazelnut extract, and 97 g of purified water were included.

실시예 2Example 2

저장성 향상을 위한 천연침지액의 제조시, 강화사자발약쑥 추출물 1g, 함초추출물 1g, 백하수오 추출물 1g, 자몽종자 추출물 0.02g과 정제수 96.98g을 포함하는 것을 제외하고 비교예 1과 동일한 방법으로 박피 도라지를 처리하였다.In the preparation of the natural soaking liquid for improving storage stability, peeled bellflower seeds were peeled in the same manner as in Comparative Example 1, except that 1 g of Ganghwasajabal medicinal mugwort extract, 1 g of glasswort extract, 1 g of white hazelnut extract, 0.02 g of grapefruit seed extract, and 96.98 g of purified water were included. was processed.

실험예Experimental example

본 발명에 따른 미생물 성장억제 및 갈변현상을 방지하는 박피 도라지의 저장상 향상 효과를 실험하기 위해 다음과 같이 실험하였다.In order to test the storage phase improvement effect of peeled bellflower, which inhibits microbial growth and prevents browning according to the present invention, the following experiments were conducted.

본 실험예에 있어서 도라지는 수확한지 얼마 되지 않은 신선한 도라지를 구입하여 사용하였다.In this experimental example, fresh bellflower seeds that have just been harvested were purchased and used.

우선 흐르는 물에서 3~4회 도라지를 세척하여 흙과 표면의 이물질을 제거한 후 박피 도구를 이용하여 도라지의 표면을 박피한 후 일정한 크기로 세절하였다.First, the bellflower was washed 3 to 4 times in running water to remove soil and foreign substances on the surface, and then the surface of the bellflower was peeled using a peeling tool, and then cut to a certain size.

본 발명에 따른 저장성 향상을 위한 천연침지액의 효과를 분석하기 위하여 비교예와 실시예에 의해 제조된 저장성 향상을 위한 천연침지액으로 처리된 박피 도라지를 즉시, 3일, 6일, 9일, 12일에 걸쳐 총 5회의 미생물수와 색도변화를 측정하였다.In order to analyze the effect of the natural immersion solution for improving storage stability according to the present invention, peeled bellflower seeds treated with the natural immersion solution for improving storage stability prepared in Comparative Examples and Examples were immediately, 3 days, 6 days, 9 days, The number of microorganisms and color change were measured a total of 5 times over 12 days.

실험예 1 박피 도라지의 갈변현상 관찰Experimental Example 1 Observation of browning of peeled bellflower

강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물, 자몽종자 추출물의 갈변 억제효과를 확인하기 위해 박피 도라지를 각각의 추출물에 침지시켜 색 변화를 관찰하였다.In order to confirm the browning inhibitory effect of Ganghwasajabalya wormwood extract, Hamcho extract, Baekhasuo extract, and Grapefruit seed extract, peeled bellflower seeds were immersed in each extract and the color change was observed.

도라지를 세라믹 칼을 이용하여 15 mm 두께로 세절한 후 조성물 총 중량에 대하여 강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물은 각각 1.0중량%, 자몽종자 추출물 0.02중량% 함유한 용액에 10분간 침지한 후 1분간 자연건조하고, 흡수지로 수분을 가볍게 제거한 후 냉장(4℃)에서 12일 동안 관찰하였다.After cutting the bellflower to a thickness of 15 mm using a ceramic knife, it was immersed for 10 minutes in a solution containing 1.0% by weight of Ganghwa Lion's Foot Artemisia extract, 1.0% by weight of Hamcho extract, and 0.02% by weight of grapefruit seed extract, respectively, based on the total weight of the composition. Then, it was naturally dried for 1 minute, and after lightly removing moisture with an absorbent paper, it was observed for 12 days in a refrigerator (4℃).

도2는 본 발명의 저장성 향상을 위한 천연침지액으로 처리한 실시예1, 실시예2와 무처리구의 박피 도라지 색상을 나타낸 것이다.Figure 2 shows the color of the peeled bellflower in Examples 1 and 2 treated with the natural immersion liquid for improving storage stability of the present invention and untreated.

실시예의 방법에 의하여 제조된 저장성 향상을 위한 천연침지액으로 처리한 우측사진에서 갈변현상 방지 효과가 우수함을 알 수 있다.It can be seen that the effect of preventing browning is excellent in the right picture treated with the natural immersion liquid for improving storage stability prepared by the method of Example.

실험예 2 박피 도라지 표면색 측정Experimental Example 2 Measurement of surface color of peeled bellflower

상기 무처리구, 실시예1~2, 비교예1~4에 의해 제조된 저장성 향상을 위한 천연침지액의 갈변현상 억제 효과를 색도변화를 통해 나타내기 위하여 박피 도라지의 표면색을 측정하였다.The surface color of the peeled bellflower was measured to show the browning inhibitory effect of the natural immersion liquid for improving storage stability prepared by the untreated group, Examples 1 to 2, and Comparative Examples 1 to 4 through color change.

색의 변화는 L값(lightness, 명도), a값(redness, 적색도), b값(yellowness, 황색도)을 측정하여 확인하였으며, L값, a값, b값을 이용한 전체 색변화를 ΔE 값으로 계산하였다. L값, a값, b값 및 ΔE 값은 화이트 타일(white tile, L=10000, a=-043 및 b=+043)로 눈금이 정해진 크로마미터(Chroma meter)(Model CR-400, Minolta Camera Co, Osaka, Japan)를 사용하여 측정하고, 수학식 1에 따라 총색차(ΔE)를 이용하여 분석하였으며 계산식은 다음과 같다.The color change was confirmed by measuring the L value (lightness), a value (redness), and b value (yellowness), and the overall color change using the L value, a value, and b value was measured by ΔE. value was calculated. The L, a, b, and ΔE values were measured using a chroma meter (Model CR-400, Minolta Camera) calibrated with white tiles (L=10000, a=-043, and b=+043). Co, Osaka, Japan), and analyzed using the total color difference (ΔE) according to Equation 1, and the calculation formula is as follows.

Figure 112022097910497-pat00001
Figure 112022097910497-pat00001

그 결과, 아래 표 1 <박피 도라지의 표면색 측정>에서 보는 바와 같이 저장성 향상을 위한 천연침지액을 처리하지 않은 박피 도라지의 L값이 52.42±0.33를 나타낸 반면, 1.0% 강화사자발약쑥 추출물은 59.50±0.55, 1.0% 함초 추출물은 57.48±0.50, 1.0% 백하수오 추출물은 57.59±0.89, 자몽종자 추출물은 55.23±0.09로 나타냈으며 특히 실시예1은 61.11±0.22, 실시예2는 60.41±0.03을 나타내어 실시예1과 실시예2로 처리한 박피 도라지가 높은 L 값을 보였다.As a result, as shown in Table 1 <Surface color measurement of peeled bellflowers>, the L value of peeled bellflowers not treated with natural immersion liquid for improving storage stability was 52.42±0.33, whereas the 1.0% strengthened lion's foot wormwood extract was 59.50± 0.55, 1.0% seaweed extract showed 57.48±0.50, 1.0% white hazelnut extract showed 57.59±0.89, and grapefruit seed extract showed 55.23±0.09. In particular, Example 1 showed 61.11±0.22, Example 2 showed 60.41±0.03, Peeled bellflower seeds treated with Examples 1 and 2 showed high L values.

L 값이 높고, a 값이 마이너스 값을 보이며, b 값이 낮을수록 갈변현상이 낮은 것으로 평가된다.The higher the L value, the negative a value, and the lower the b value, the lower the browning phenomenon.

또한, 12일 저장시간 기준으로 ΔE 값은 추출물로 처리하지 않은 박피 도라지에서 116.61±1.35의 색변화를 나타낸 반면, 실시예1로 처리한 경우는 13.00±0.53, 실시예2로 처리한 경우는 4.79±0.33의 색변화를 나타내 추출물 혼합액에서 색 변화가 낮게 나타남을 알 수 있었다.In addition, based on the storage time of 12 days, the ΔE value showed a color change of 116.61 ± 1.35 in the peeled bellflower not treated with the extract, whereas in the case of Example 1, it was 13.00 ± 0.53, and in the case of Example 2, it was 4.79. It was found that the color change of ± 0.33 was shown to be low in the extract mixture.

구 분division 천연 침지액 조성물 성분Natural Immersion Liquid Composition Ingredients 저장시간
(days)
storage time
(days)
Hunter’s valueHunter's value
L (Lightness)L (Lightness) a (redness)a (redness) b (yellowness)b (yellowness) ΔEΔE 무처리구untreated -- 00 62.11±1.1662.11±1.16 -0.01±0.41-0.01±0.41 7.50±1.857.50±1.85 -- 33 60.91±1.06 60.91±1.06 0.16±0.080.16±0.08 12.15±1.9212.15±1.92 11.53±0.7711.53±0.77 66 59.61±1.1859.61±1.18 0.52±0.310.52±0.31 14.40±1.5814.40±1.58 27.02±0.0427.02±0.04 99 55.83±1.3555.83±1.35 2.14±1.072.14±1.07 16.21±5.7816.21±5.78 59.94±7.9559.94±7.95 1212 52.42±0.3352.42±0.33 3.69±1.713.69±1.71 18.71±2.3918.71±2.39 116.61±1.35116.61±1.35 실시예1Example 1 비교예1+비교예2
+비교예3
Comparative Example 1 + Comparative Example 2
+Comparative Example 3
00 61.78±0.1361.78±0.13 -0.01±0.41-0.01±0.41 6.65±1.176.65±1.17 --
33 60.18±0.2260.18±0.22 -0.11±0.02-0.11±0.02 6.49±0.186.49±0.18 1.30±1.851.30±1.85 66 60.01±0.4060.01±0.40 0.08±0.090.08±0.09 7.82±0.707.82±0.70 2.25±0.202.25±0.20 99 56.92±0.2256.92±0.22 -0.07±0.14-0.07±0.14 6.10±0.906.10±0.90 11.96±0.0811.96±0.08 1212 61.11±0.2261.11±0.22 0.94±0.190.94±0.19 11.61±0.1811.61±0.18 13.00±0.5313.00±0.53 실시예2Example 2 비교예4+실시예1Comparative Example 4 + Example 1 00 60.78±0.1360.78±0.13 -0.01±0.41-0.01±0.41 7.50±1.857.50±1.85 -- 33 60.72±0.0060.72±0.00 0.75±0.200.75±0.20 9.13±0.819.13±0.81 1.62±0.571.62±0.57 66 60.26±0.0260.26±0.02 0.96±0.160.96±0.16 9.32±0.379.32±0.37 2.25±0.992.25±0.99 99 58.05±0.2558.05±0.25 0.27±0.540.27±0.54 8.16±2.878.16±2.87 3.98±1.023.98±1.02 1212 60.41±0.0360.41±0.03 1.02±0.371.02±0.37 10.04±0.1410.04±0.14 4.79±0.334.79±0.33 비교예1Comparative Example 1 1.0%
강화사자발약쑥
추출물
1.0%
Ganghwa Lion's Foot Wormwood
extract
00 62.11±1.1662.11±1.16 -0.01±0.41-0.01±0.41 7.50±1.857.50±1.85 --
33 59.51±0.4159.51±0.41 0.74±0.240.74±0.24 8.39±1.078.39±1.07 4.05±3.694.05±3.69 66 60.29±0.2860.29±0.28 0.74±0.230.74±0.23 8.53±0.628.53±0.62 2.45±1.162.45±1.16 99 57.44±0.7157.44±0.71 -0.02±0.42-0.02±0.42 8.39±1.848.39±1.84 11.28±0.1011.28±0.10 1212 59.50±0.5559.50±0.55 1.63±0.211.63±0.21 11.40±0.7511.40±0.75 12.34±0.8112.34±0.81 비교예2Comparative Example 2 1.0%
함초 추출물
1.0%
Glasswort extract
00 62.11±1.1662.11±1.16 -0.01±0.41-0.01±0.41 7.50±1.857.50±1.85 --
33 59.30±0.2459.30±0.24 0.22±0.230.22±0.23 8.28±0.588.28±0.58 4.29±4.784.29±4.78 66 59.44±1.7459.44±1.74 0.54±0.440.54±0.44 8.61±1.278.61±1.27 4.33±0.344.33±0.34 99 56.79±1.1856.79±1.18 -0.58±0.05-0.58±0.05 4.93±0.354.93±0.35 17.62±1.1917.62±1.19 1212 57.48±0.5057.48±0.50 1.15±0.491.15±0.49 11.67±2.1011.67±2.10 20.09±0.2520.09±0.25 비교예3Comparative Example 3 1.0%
백하수오
추출물
1.0%
baekhasoo
extract
00 62.11±1.1662.11±1.16 -0.01±0.41-0.01±0.41 7.50±1.857.50±1.85 --
33 58.23±0.9858.23±0.98 0.56±0.060.56±0.06 7.25±0.067.25±0.06 7.72±9.197.72±9.19 66 58.13±0.5158.13±0.51 1.23±0.341.23±0.34 9.44±1.179.44±1.17 10.57±0.4510.57±0.45 99 56.49±0.2656.49±0.26 -0.22±0.28-0.22±0.28 6.79±0.016.79±0.01 16.09±2.1016.09±2.10 1212 57.59±0.8957.59±0.89 2.01±0.202.01±0.20 12.77±1.3712.77±1.37 26.12±0.1726.12±0.17 비교예4Comparative Example 4 0.02%
자몽종자
추출물
0.02%
grapefruit seed
extract
00 61.44±0.4961.44±0.49 -0.01±0.41-0.01±0.41 7.50±1.857.50±1.85 --
33 59.55±0.0059.55±0.00 0.62±0.030.62±0.03 7.70±0.007.70±0.00 2.01±3.572.01±3.57 66 57.95±0.8257.95±0.82 0.91±0.000.91±0.00 7.65±0.087.65±0.08 6.54±1.716.54±1.71 99 55.85±0.9155.85±0.91 -0.31±0.35-0.31±0.35 5.21±0.595.21±0.59 18.34±0.8918.34±0.89 1212 55.23±0.0955.23±0.09 0.84±0.260.84±0.26 10.32±0.7710.32±0.77 23.62±0.6823.62±0.68

실험예3 PPO의 활성저해도 측정Experimental Example 3 Measurement of activity inhibition of PPO

50 mM phosphate buffer(pH 6.5) 1.7 ㎖와 PPO(4,276 units/㎎) 0.2 ㎖를 혼합한 후 0.5%와 1% 농도의 저해제를 0.1㎖를 첨가하여 25℃로 조절된 항온수조에서 15분간 방치하고 기질로서 4 mM catechin 용액 1 ㎖를 각각 첨가하고, 분광광도계(Cary300, Spectrophotometer, USA)를 이용하여 420 nm에서 5분간의 변화를 측정하면 표 2의 <PPO 활성저해도 측정결과>와 같다.After mixing 1.7 ml of 50 mM phosphate buffer (pH 6.5) and 0.2 ml of PPO (4,276 units/mg), 0.1 ml of 0.5% and 1% inhibitors were added and left in a constant temperature water bath adjusted to 25 ° C for 15 minutes. When 1 ml of 4 mM catechin solution was added as a substrate, and the change at 420 nm for 5 minutes was measured using a spectrophotometer (Cary300, Spectrophotometer, USA), Table 2 shows <PPO activity inhibition degree measurement result>.

효소의 활성능은 흡광도의 변화를 관찰한 후 curve의 직선부위로부터 계산하였으며, 효소 저해활성은 흡광도 감소량 %로 나타내었다.(Dennis & Miller 1998)The activity of the enzyme was calculated from the linear portion of the curve after observing the change in absorbance, and the enzyme inhibitory activity was expressed as the percentage of decrease in absorbance. (Dennis & Miller 1998)

그리고, 비교예1~4, 실시예1~2에 의해 제조된 저장성 향상을 위한 천연침지액의 PPO 저해활성 값으로 하였다.In addition, the PPO inhibitory activity value of the natural immersion liquid for improving storage stability prepared by Comparative Examples 1 to 4 and Examples 1 to 2 was used.

Inhibition of PPO activity(%)=[1-(A/B)]×100Inhibition of PPO activity (%)=[1-(A/B)]×100

(A : sample의 흡광도, B : blank solution의 흡광도)(A: absorbance of sample, B: absorbance of blank solution)

농도(%)density(%) 활성저해도(%)Activity inhibition degree (%) 비교예1Comparative Example 1 0.50.5 16.3216.32 1One 32.4232.42 비교예2Comparative Example 2 0.50.5 14.3314.33 1One 32.2132.21 비교예3Comparative Example 3 0.50.5 13.6713.67 1One 29.0329.03 비교예4Comparative Example 4 0.50.5 12.2312.23 1One 28.9828.98 실시예1Example 1 0.50.5 19.3419.34 1One 37.5637.56 실시예2Example 2 0.50.5 20.8120.81 1One 42.6042.60

PPO 활성 저해력은 실시예2 > 실시예1 > 비교예1 > 비교예2 > 비교예3 > 비교예 4순인 것으로 파악되었다.The PPO activity inhibition was found to be in the order of Example 2 > Example 1 > Comparative Example 1 > Comparative Example 2 > Comparative Example 3 > Comparative Example 4.

실험예4 박피 도라지의 미생물수 측정Experimental Example 4 Measurement of the number of microorganisms in peeled bellflower

본 발명의 비교예, 실시예에 따른 저장성 향상을 위한 천연침지액 225g에 세절한 박피 도라지 25g을 넣어 침지한 후 냉장(4℃)에서 12일간 보관하면서 3일 간격으로 일반세균수, 대장균군수, 진균수의 변화를 측정하였다.After immersing 25 g of peeled bellflower root in 225 g of natural immersion liquid for improving storage stability according to Comparative Examples and Examples of the present invention, immersed in refrigerated (4 ℃) for 12 days, general bacterial count, coliform count, Changes in the number of fungi were measured.

미생물수 측정은 침지액을 1mL씩 취하여 9mL의 멸균된 saline 용액으로 단계 희석하였다. 각 단계 희석액 1mL을 일반세균수 측정용 건조필름(petrifilm aerobic count plates, 3M Co, USA), 대장균 및 대장균군수 측정용 건조필름(petrifilm Ecoli/coliform count plates, 3M Co, USA), 진균수 측정용 건조필름(petrifilm yeast/mold count plates, 3M Co, USA)에 무균적으로 취하여 일반세균, 대장균 및 대장균군은 35±1에서 48시간 배양하고 진균은 25℃에서 5일간 배양한 후 형성된 colony 수를 측정하여 CFU/g 단위로 나타내었다.For the measurement of the number of microorganisms, 1 mL of the immersion solution was diluted step by step with 9 mL of a sterilized saline solution. 1mL of each dilution solution was used for measuring general bacterial counts (petrifilm aerobic count plates, 3M Co, USA), dry films for measuring E. coli and coliform count plates (petrifilm Ecoli/coliform count plates, 3M Co, USA), and for measuring the number of fungi Aseptically taken on dry films (petrifilm yeast/mold count plates, 3M Co, USA), general bacteria, E. coli, and coliforms were cultured at 35±1 for 48 hours, and fungi were cultured at 25℃ for 5 days, and the number of colonies formed was counted. It was measured and expressed in terms of CFU/g.

표 3은 본 발명에 따른 박피 도라지를 저장성 향상을 위한 천연침지액에 침지하여 저장기간에 따른 일반세균수의 변화를 나타낸 것이다. 일반세균수의 측정단위는 CFU/g 이다Table 3 shows the change in the number of general bacteria according to the storage period by immersing the peeled bellflower according to the present invention in a natural immersion liquid to improve storage stability. The unit of measurement for the normal bacterial count is CFU/g.

구분division Storage period(days)Storage period (days) 00 33 66 99 1212 무처리구untreated 3.5 x 104 3.5 x 10 4 1.8 x 106 1.8 x 10 6 1.4 x 107 1.4 x 10 7 2.9 x 108 2.9 x 10 8 6.7 x 108 6.7 x 10 8 비교예1Comparative Example 1 4.4 x 104 4.4 x 10 4 9.2 x 105 9.2 x 10 5 3.9 x 106 3.9 x 10 6 1.3 x 108 1.3 x 10 8 1.0 x 108 1.0 x 10 8 비교예2Comparative Example 2 3.2 x 104 3.2 x 10 4 5.5 x 105 5.5 x 10 5 7.0 x 106 7.0 x 10 6 1.6 x 108 1.6 x 10 8 1.3 x 108 1.3 x 10 8 비교예3Comparative Example 3 4.9 x 104 4.9 x 10 4 6.4 x 105 6.4 x 10 5 7.9 x 106 7.9 x 10 6 1.4 x 108 1.4 x 10 8 2.8 x 108 2.8 x 10 8 비교예4Comparative Example 4 3.4 x 104 3.4 x 10 4 7.1 x 105 7.1 x 10 5 2.3 x 106 2.3 x 10 6 6.3 x 107 6.3 x 10 7 8.2 x 107 8.2 x 10 7 실시예1Example 1 2.8 x 104 2.8 x 10 4 2.9 x 105 2.9 x 10 5 3.6 x 106 3.6 x 10 6 7.3 x 107 7.3 x 10 7 9.4 x 107 9.4 x 10 7 실시예2Example 2 2.3 x 104 2.3 x 10 4 2.2 x 105 2.2 x 10 5 1.8 x 106 1.8 x 10 6 4.6 x 107 4.6 x 10 7 4.1 x 107 4.1 x 10 7

표 3에서 알 수 있듯이, 저장일수가 증가함에 따라 박피 도라지의 일반세균수는 전반적으로 증가하는 경향을 보였으나 본 발명에 따른 실시예1과 실시예2의 일반세균수 측정값은 무처리구의 일반세균수보다 낮게 측정되었다. 따라서, 본 발명에 따른 저장성 향상을 위한 천연침지액은 일반세균수의 감소에 기여함을 확인할 수 있다.As can be seen in Table 3, as the storage days increased, the number of general bacteria of peeled bellflower tended to increase overall, but the measured values of the number of general bacteria in Examples 1 and 2 according to the present invention showed that the general bacteria in the untreated group measured lower than the number of Therefore, it can be confirmed that the natural immersion liquid for improving storage stability according to the present invention contributes to the reduction of the number of general bacteria.

표 4는 본 발명에 따른 박피 도라지를 저장성 향상을 위한 천연침지액에 침지하여 저장기간에 따른 대장균군수의 변화를 나타낸 것이다.Table 4 shows the change in the number of coliform counts according to the storage period by immersing the peeled bellflower according to the present invention in a natural immersion liquid for improving storage stability.

본 실험에서 대장균은 검출되지 않았다. 대장균군수의 측정단위는 CFU/g 이다Escherichia coli was not detected in this experiment. The unit of measurement for coliform count is CFU/g.

구분division Storage period(days)Storage period (days) 00 33 66 99 1212 무처리구untreated 3.7 x 103 3.7 x 10 3 3.2 x 104 3.2 x 10 4 8.6 x 104 8.6 x 10 4 4.3 x 105 4.3 x 10 5 7.9 x 105 7.9 x 10 5 비교예1Comparative Example 1 2.8 x 103 2.8 x 10 3 1.8 x 104 1.8 x 10 4 5.9 x 104 5.9 x 10 4 2.5 x 105 2.5 x 10 5 4.6 x 105 4.6 x 10 5 비교예2Comparative Example 2 1.6 x 103 1.6 x 10 3 2.3 x 104 2.3 x 10 4 4.4 x 104 4.4 x 10 4 3.8 x 105 3.8 x 10 5 5.1 x 105 5.1 x 10 5 비교예3Comparative Example 3 1.2 x 103 1.2 x 10 3 3.5 x 104 3.5 x 10 4 6.9 x 104 6.9 x 10 4 3.4 x 105 3.4 x 10 5 3.2 x 105 3.2 x 10 5 비교예4Comparative Example 4 1.1 x 103 1.1 x 10 3 3.4 x 104 3.4 x 10 4 4.3 x 104 4.3 x 10 4 1.7 x 105 1.7 x 10 5 1.2 x 105 1.2 x 10 5 실시예1Example 1 1.2 x 103 1.2 x 10 3 8.1 x 103 8.1 x 10 3 1.4 x 104 1.4 x 10 4 7.9 x 104 7.9 x 10 4 8.5 x 104 8.5 x 10 4 실시예2Example 2 1.5 x 103 1.5 x 10 3 5.7 x 103 5.7 x 10 3 7.1 x 103 7.1 x 10 3 2.7 x 104 2.7 x 10 4 5.3 x 104 5.3 x 10 4

표 4에서 알 수 있듯이, 저장일수가 증가함에 따라 박피 도라지의 대장균군수는 전반적으로 증가하는 경향을 보였으나 본 발명에 따른 실시예1과 실시예2의 대장균군수 측정값은 무처리구의 대장균군수보다 낮게 측정되었다.As can be seen in Table 4, as the storage days increased, the number of coliforms in peeled bellflower seeds tended to increase overall, but the measured values of the number of coliforms in Examples 1 and 2 according to the present invention were lower than those in the untreated group. has been measured

따라서, 본 발명에 따른 저장성 향상을 위한 천연침지액은 대장균군수의 감소에 기여함을 확인할 수 있다.Therefore, it can be confirmed that the natural immersion liquid for improving storage stability according to the present invention contributes to the reduction of the coliform count.

표 5는 본 발명에 따른 박피 도라지를 저장성 향상을 위한 천연침지액에 침지하여 저장기간에 따른 진균수의 변화를 나타낸 것이다. 진균수의 측정단위는 CFU/g 이다Table 5 shows the change in the number of fungi according to the storage period by immersing the peeled bellflower according to the present invention in a natural immersion liquid for improving storage stability. The unit of measure for the number of fungi is CFU/g.

구분division Storage period(days)Storage period (days) 00 33 66 99 1212 무처리구untreated 6.9 x 102 6.9 x 10 2 3.2 x 103 3.2 x 10 3 2.8 x 104 2.8 x 10 4 4.6 x 104 4.6 x 10 4 8.9 x 104 8.9 x 10 4 비교예1Comparative Example 1 3.3 x 102 3.3 x 10 2 5.8 x 103 5.8 x 10 3 1.4 x 104 1.4 x 10 4 3.5 x 104 3.5 x 10 4 4.9 x 104 4.9 x 10 4 비교예2Comparative Example 2 6.2 x 102 6.2 x 10 2 4.2 x 103 4.2 x 10 3 2.3 x 104 2.3 x 10 4 4.1 x 104 4.1 x 10 4 6.2 x 104 6.2 x 10 4 비교예3Comparative Example 3 2.7 x 102 2.7 x 10 2 8.6 x 103 8.6 x 10 3 1.7 x 104 1.7 x 10 4 3.3 x 104 3.3 x 10 4 2.7 x 104 2.7 x 10 4 비교예4Comparative Example 4 2.0 x 102 2.0 x 10 2 7.1 x 103 7.1 x 10 3 1.2 x 104 1.2 x 10 4 2.8 x 104 2.8 x 10 4 2.3 x 104 2.3 x 10 4 실시예1Example 1 1.8 x 102 1.8 x 10 2 1.1 x 103 1.1 x 10 3 3.9 x 103 3.9 x 10 3 2.5 x 103 2.5 x 10 3 8.2 x 103 8.2 x 10 3 실시예2Example 2 3.4 x 102 3.4 x 10 2 1.0 x 103 1.0 x 10 3 2.7 x 103 2.7 x 10 3 2.3 x 103 2.3 x 10 3 5.4 x 103 5.4 x 10 3

본 발명에 따른 실시예1과 실시예2 진균수 측정값은 무처리구의 진균수보다 낮게 측정되었다. 따라서, 본 발명에 따른 저장성 향상을 위한 천연침지액은 진균수의 감소에 기여함을 확인할 수 있다.The measured values of the number of fungi in Examples 1 and 2 according to the present invention were measured lower than the number of fungi in the untreated group. Therefore, it can be confirmed that the natural immersion liquid for improving storage stability according to the present invention contributes to the reduction of the number of fungi.

전술한 바와 같이 본 발명에 의해 제조된 저장성 향상을 위한 천연침지액은 도라지와 같은 박피 근채류의 미생물 성장 및 갈변현상을 최대한 억제할 수 있고 고유의 맛과 향이 지속되고 영양소 파괴를 최소화시켜 소비자가 원하는 제품을 제공할 수 있을 것으로 기대될 수 있다.As described above, the natural immersion liquid for improving storage stability prepared by the present invention can suppress microbial growth and browning of peeled root vegetables such as bellflower as much as possible, maintains the original taste and aroma, and minimizes nutrient destruction, which is what consumers want You can expect to be able to provide products.

본 발명이 속하는 기술분야의 당업자는 상술한 본 발명이 그 기술적 사상이나 필수적 구성을 변경하지 않고서 다른 구체적인 형태로 실시될 수 있다는 것을 이해할 수 있을 것이다.Those skilled in the art to which the present invention pertains will be able to understand that the above-described present invention can be implemented in other specific forms without changing the technical spirit or essential configuration.

그러므로, 이상에서 기술한 실시예들은 모든 면에서 예시적인 것이며 한정적이 아닌 것으로 이해하여야 한다. 본 발명의 범위는 상기 상세한 설명보다는 후술하는 특허청구범위에 의하여 나타내어지며 특허청구범위의 의미 및 범위 그리고 등가 개념으로부터 도출되는 모든 변경 또는 형태가 본 발명의 범위에 포함되는 것으로 해석되어야 한다.Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. The scope of the present invention is indicated by the claims to be described later rather than the detailed description above, and all changes or forms derived from the meaning and scope of the claims and equivalent concepts should be construed as being included in the scope of the present invention.

본 발명은 천연 추출물을 함유하는 박피 도라지의 저장성을 향상시키는 천연침지액에 관한 것으로서, 보다 구체적으로는 강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물 및 자몽종자 추출물을 함유하며 미생물 성장억제 및 갈변방지 효과가 있는 박피 도라지의 저장성 향상을 위한 천연침지액에 관한 것이다.The present invention relates to a natural soaking liquid for improving the storability of peeled bellflowers containing natural extracts, and more specifically, to a natural soaking liquid containing Ganghwa lion's foot mugwort extract, glasswort extract, white hazelnut extract, and grapefruit seed extract, which inhibits microbial growth and prevents browning. It relates to a natural immersion liquid for improving the storage stability of peeled bellflowers, which is effective.

Claims (4)

강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물 및 자몽종자 추출물을 유효성분으로 포함하는 박피 도라지의 저장성 향상을 위한 천연침지액.
A natural soaking liquid for improving storage stability of peeled bellflower, containing Ganghwasajabalak mugwort extract, glasswort extract, baekhasuo extract, and grapefruit seed extract as active ingredients.
제1항에 있어서,
천연침지액 조성물 총 중량에 대해 상기 강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물은 각 1.0중량%, 자몽종자 추출물은 0.02중량% 포함됨을 특징으로 하는 박피 도라지의 저장성 향상을 위한 천연침지액.
According to claim 1,
A natural soaking liquid for improving the shelf life of peeled bellflowers, characterized in that each of the Ganghwa lion's foot wormwood extract, glasswort extract, and white hazelnut extract contains 1.0% by weight and grapefruit seed extract of 0.02% by weight, based on the total weight of the natural soaking liquid composition.
강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물 및 자몽종자 추출물을 포함하는 박피 도라지의 저장성 향상을 위한 천연침지액에 박피 도라지를 침지하여 포장하는 것을 특징으로 하는 박피 도라지의 저장성 향상 방법.
A method for improving the storage stability of peeled bellflower, characterized in that the peeled bellflower is immersed and packaged in a natural immersion liquid for improving the storage stability of peeled bellflower containing Ganghwasajabal wormwood extract, seaweed extract, white hazelnut extract and grapefruit seed extract.
(1) 도라지를 선별, 세척하여 준비한 후 절단하여 도라지 절편을 제조하는 단계;
(2) 강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물을 제조하는 단계;
(3) 강화사자발약쑥 추출물, 함초 추출물, 백하수오 추출물 및 자몽종자 추출물을 포함하여 저장성 향상을 위한 천연침지액을 제조하는 단계;
(4) 상기 제조한 박피 도라지를 상기 저장성 향상 천연침지액에 침지 처리하는 단계;
(5) 냉장(4℃)에서 저장 및 보관하는 단계;를 포함하는 박피 도라지의 저장성 향상 방법.
(1) selecting, washing, preparing, and then cutting to prepare bellflower slices;
(2) preparing Ganghwa lion's foot wormwood extract, glasswort extract, and baekhasuo extract;
(3) preparing a natural soaking liquid for improving storage stability, including Ganghwasajabal medicinal mugwort extract, glasswort extract, baekhasuo extract, and grapefruit seed extract;
(4) immersing the prepared peeled bellflower in the storage-enhancing natural immersion liquid;
(5) storage and storage in refrigeration (4 ℃); storage improvement method of peeled bellflower, including.
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