KR102512999B1 - 금 나노 입자-DNA 복합체 및 CRISPR/Cas12a 복합체를 포함하는 바이오센서, 및 이를 이용한 표적 DNA 검출 방법 - Google Patents

금 나노 입자-DNA 복합체 및 CRISPR/Cas12a 복합체를 포함하는 바이오센서, 및 이를 이용한 표적 DNA 검출 방법 Download PDF

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KR102512999B1
KR102512999B1 KR1020210053086A KR20210053086A KR102512999B1 KR 102512999 B1 KR102512999 B1 KR 102512999B1 KR 1020210053086 A KR1020210053086 A KR 1020210053086A KR 20210053086 A KR20210053086 A KR 20210053086A KR 102512999 B1 KR102512999 B1 KR 102512999B1
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dna
stranded dna
gold nanoparticle
crispr
gold
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KR1020210053086A
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KR20220146162A (ko
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백세환
최정우
최진하
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솔바이오 주식회사
서강대학교산학협력단
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Priority to KR1020210053086A priority Critical patent/KR102512999B1/ko
Priority to PCT/KR2021/015153 priority patent/WO2022225114A1/fr
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
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    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/137—Metal/ion, e.g. metal label
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/155—Particles of a defined size, e.g. nanoparticles

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
KR1020210053086A 2021-04-23 2021-04-23 금 나노 입자-DNA 복합체 및 CRISPR/Cas12a 복합체를 포함하는 바이오센서, 및 이를 이용한 표적 DNA 검출 방법 KR102512999B1 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
KR1020210053086A KR102512999B1 (ko) 2021-04-23 2021-04-23 금 나노 입자-DNA 복합체 및 CRISPR/Cas12a 복합체를 포함하는 바이오센서, 및 이를 이용한 표적 DNA 검출 방법
PCT/KR2021/015153 WO2022225114A1 (fr) 2021-04-23 2021-10-27 Biocapteur comprenant un complexe nanoparticule d'or-adn et un complexe crispr/cas12a, et procédé de détection d'adn cible à l'aide de celui-ci

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KR1020210053086A KR102512999B1 (ko) 2021-04-23 2021-04-23 금 나노 입자-DNA 복합체 및 CRISPR/Cas12a 복합체를 포함하는 바이오센서, 및 이를 이용한 표적 DNA 검출 방법

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KR20220146162A KR20220146162A (ko) 2022-11-01
KR102512999B1 true KR102512999B1 (ko) 2023-03-23

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WO (1) WO2022225114A1 (fr)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102086282B1 (ko) 2019-01-02 2020-03-06 서강대학교산학협력단 금속 나노입자-핵산-펩타이드 복합체를 이용한 단백질 분해 효소 검출용 센서

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10253365B1 (en) * 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102086282B1 (ko) 2019-01-02 2020-03-06 서강대학교산학협력단 금속 나노입자-핵산-펩타이드 복합체를 이용한 단백질 분해 효소 검출용 센서

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BIOLOGY METHODS AND PROTOCOLS, 2020, 1-7 [DOI: 10.1093/BIOMETHODS/BPAA020]
J. AM. CHEM. SOC. 2020, VOL.142, P. 7506-7513 [DOI.ORG/10.1021/JACS.0C00217]
JOURNAL OF NANOBIOTECHNOLOGY 2011, 9:16 [DOI:10.1186/1477_3155_9_16]
NANO LETT. 2020, 20, 7100_7107 [.DOI.ORG/10.1021/ACS.NANOLETT.0C02343]
NANOPHOTONICS 2017; VOL. 6(1), P. 71-92 [DOI 10.1515/NANOPH_2015_0143]

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WO2022225114A1 (fr) 2022-10-27

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