KR102508584B1 - Antibacterial paper for breaking dormancy for plant seed germination test - Google Patents

Abstract

The present invention relates to antibacterial paper for breaking dormancy for plant seed germination test. More particularly, it can be utilized for mass propagation by examining whether to break the dormancy of a plant seed by a gibberellin (GA) hormone layer, and can minimize pathogen contamination by endogenous bacteria from a non-sterilized seed. The antibacterial paper includes: a base layer; a first gibberellin layer; a second gibberellin layer; and a second antibacterial layer.

Description

Antibacterial paper for breaking dormancy for plant seed germination test}

The present invention relates to antibacterial paper for breaking dormancy for plant seed germination assay, and more particularly, can be used for germination rate test by quickly examining whether dormancy breaking by a gibberellin (GA) hormone layer in an airplane, and can be used for germination rate test. It relates to antibacterial paper for breaking dormancy for germination assay of plant seeds that can minimize contamination from non-sterilized seeds.

The use of native plants is recommended for the success of afforestation and planting for forest restoration, forest reclamation, prevention of global warming, and gene protection of neighboring species. Seed transfer zone), etc. are set up and utilized actively.

Partial revision of the ‘Act on the Creation and Management of Forest Resources’ (2020.09) Notification of the supply list of native plants for forest restoration makes the use of native plants mandatory during forest restoration, and prepares forest restoration strategies and capabilities for ecological restoration and maintenance of a healthy forest ecosystem Reinforcement, comprehensive system and foundation establishment are required.

The need for localization of native plant seed production for forest restoration is emerging due to the increase in alien species, ecosystem disturbance, and management cost increase due to seed imports.

However, due to the absence of systematic systems such as seed collection, cultivation, management, and standardized production methods, consistent quality assurance, mass production/industrialization information, and technical foundations are generally lacking. In particular, there is a serious lack of standardized technology such as the characteristics of domestic native plant seeds (germination rate, germination and growth under various conditions, etc.) and cultivation methods. For successful forest restoration, it is required to present guidelines for seed migration through seed characteristics research, selection and use of native plant materials.

In addition, in the case of domestic native plant seeds, it is difficult to present guidelines and apply them to the field due to the lack of basic information (germination rate, weight, dormancy type) of various wild plant seeds.

Therefore, this research and development invented a method that can efficiently germinate and screen various wild plant seeds in a short time by utilizing hormone (gibberellin), which is one of the general methods of inducing seed dormancy breakage.

Republic of Korea Patent Publication No. 10-2015-0045611

The present invention has been made to solve the above conventional problems,

Dormancy for plant seed germination assay, which can be used for mass propagation by examining whether plant seeds are dormant broken by the gibberellin (GA) hormone layer, and can minimize pathogen contamination by endogenous bacteria from non-sterilized seeds by the antibacterial layer. An object of the present invention is to provide antibacterial paper for tapa.

In order to achieve the above object, the present invention is a substrate and;

a first gibberellin layer provided on one surface of the substrate by physical vapor deposition to induce germination of seeds by inducing dormancy breaking of plant seeds;

A first antibacterial layer provided on the substrate or the first gibberellin layer by a physical vapor deposition method;

It relates to an antibacterial paper for breaking dormancy for plant seed germination assay, characterized in that it comprises a.

In addition, a second gibberellin layer provided on the other surface of the substrate of the present invention by physical vapor deposition to induce germination of seeds by inducing dormancy breaking of plant seeds;

A second antibacterial layer provided on the other surface of the substrate or the second gibberellin layer by physical vapor deposition;

It relates to an antibacterial paper for breaking dormancy for plant seed germination assay, characterized in that it further comprises.

In addition, the substrate of the present invention relates to an antibacterial paper for breaking dormancy for plant seed germination assay, characterized in that any one of paper, nonwoven fabric, fiber, film, and biodegradable plastic is selected and used.

In addition, the first antibacterial layer and the second antibacterial layer of the present invention contain any one or two or more alloys selected from copper, zinc, palladium, platinum, gold and silver, break dormancy for plant seed germination assay It is about antibacterial paper for use.

In addition, the physical vapor deposition method of the present invention relates to antibacterial paper for breaking dormancy for plant seed germination assay, characterized in that it is deposited by a sputter device.

In addition, the sputtering device of the present invention is provided with a sputtering target for depositing a gibberellin layer and an antibacterial layer inside the sputtering chamber, and after providing the substrate to be spaced apart from the sputtering target, through a magnet array provided inside the sputtering chamber. It relates to antibacterial paper for breaking dormancy for plant seed germination assay, characterized in that a magnetic field is performed in the same direction on the substrate.

As described above, the antibacterial paper for breaking dormancy for plant seed germination assay of the present invention can be used for mass propagation by examining the dormancy breaking of plant seeds by the gibberellin (GA) hormone layer. There is an effect of minimizing pathogen contamination by endogenous bacteria from non-sterilized seeds.

1 is a plan view showing an antibacterial paper for breaking dormancy for plant seed germination assay according to a first embodiment of the present invention;
Figure 2 is a cross-sectional view showing part AA of Figure 1,
3 is a plan view showing an antibacterial paper for breaking dormancy for plant seed germination assay according to a second embodiment of the present invention;
4 is a cross-sectional view showing a portion BB of FIG. 3;
5 is a front view showing a roll-to-roll deposition apparatus according to an embodiment of the present invention;
6 is a photographic diagram showing a comparative experiment on seed germination using antibacterial paper for breaking dormancy for plant seed germination assay according to an embodiment of the present invention.

The present invention having such characteristics will be more clearly explained through the preferred embodiments thereof.

Before describing various embodiments of the present invention in detail with reference to the accompanying drawings, it will be appreciated that the application is not limited to the details of the configuration and arrangement of components described in the following detailed description or shown in the drawings. will be. The invention is capable of being implemented and practiced in other embodiments and of being carried out in various ways. Also, device or element orientation (e.g., "front", "back", "up", "down", "top", "bottom") The expressions and predicates used herein with respect to terms such as ", "left", "right", "lateral", etc. are only used to simplify the description of the present invention and related devices. Or it will be appreciated that it does not indicate or imply that an element simply must have a particular orientation. Also, terms such as “first” and “second” are used herein and in the appended claims for descriptive purposes and are not intended to indicate or imply relative importance or significance.

Therefore, since the embodiments described in this specification and the configurations shown in the drawings are only one of the most preferred embodiments of the present invention and do not represent all of the technical ideas of the present invention, various alternatives may be used at the time of this application. It should be understood that there may be equivalents and variations.

1 is a plan view showing an antibacterial paper for breaking dormancy for plant seed germination assay according to a first embodiment of the present invention, FIG. 2 is a cross-sectional view showing part A-A of FIG. 1, and FIG. 3 is a second embodiment of the present invention. It is a plan view showing an antibacterial paper for breaking dormancy for plant seed germination test according to the example, FIG. 4 is a cross-sectional view showing a part B-B in FIG. 3, and FIG. 5 is a front view showing a roll-to-roll deposition apparatus according to an embodiment of the present invention. 6 is a photographic diagram showing a comparative experiment on seed germination using antibacterial paper for breaking dormancy for plant seed germination assay according to an embodiment of the present invention.

As shown in Figures 1 to 6, the antibacterial paper for breaking dormancy for plant seed germination assay of the present invention includes a substrate 100; a first gibberellin layer 210 provided on one surface of the substrate 100 by physical vapor deposition to induce germination of seeds by inducing dormancy breaking of plant seeds; It consists of a first antibacterial layer 310 provided by a physical vapor deposition method on the substrate 100 or the first gibberellin layer 210.

Here, as shown in FIGS. 3 and 4, a second gibberellin layer 220 provided on the other surface of the base material 100 by a physical vapor deposition method to induce dormancy breaking of plant seeds to induce germination of seeds; A second antimicrobial layer 320 provided by a physical vapor deposition method on the other surface of the substrate 100 or on the second gibberellin layer 220 is further included.

At this time, as shown in FIGS. 2 and 4, the gibberellin layer 200 and the antibacterial layer 300 are selectively provided on one or both sides of the substrate 100, and the antibacterial paper for breaking dormancy for the plant seed germination assay is selectively Optionally, a graphter layer 400 may be further included.

The substrate 100 may be selected from among paper, nonwoven fabric, fiber, film, and biodegradable plastic. In addition, the substrate 100 may include a fabric or the like, but is not limited thereto. At this time, when paper, fabric, etc. are used as the substrate 100, it may have air permeability.

Here, in the case of using the sputtering method as a physical vapor deposition method to prepare the gibberellin layer 200, the antibacterial layer 300, the carbon layer 400, etc. on the substrate 100, plasma is applied to the surface of the substrate 100 during the sputtering process. Since treatment is possible, the surface treatment process can be omitted.

The gibberellin layer 200 is divided into a first gibberellin layer 210 and a second gibberellin layer 220. ) is formed on the other surface of the substrate 100 by physical vapor deposition, respectively.

Here, since gibberellin exhibits a strong effect on the flowering phenomenon that is not greatly affected by auxin, when gibberellin is treated under a single condition, flowering of the plant is induced. In addition, gibberellins cause dormant seeds or buds to wake up from dormancy and start growing, and for some plants, they produce fruit or promote fruit growth. can induce

The antibacterial layer 300 is divided into a first antibacterial layer 310 and a second antibacterial layer 320, the first antibacterial layer 310 is one surface of the substrate 100 or the first gibberellin layer 210 Above, the second antibacterial layer 320 is formed on the other surface of the substrate 100 or the second gibberellin layer 220 by physical vapor deposition, respectively.

Here, the first antibacterial layer 310 and the second antibacterial layer 320 may include a metal layer (not shown) provided by a physical vapor deposition method, the metal layer is copper, zinc, palladium, gold, platinum and silver It includes any one or two or more alloys selected from among.

Copper (Cu) has an antibacterial function, and the antibacterial principle of copper can be explained by the fact that when bacteria, viruses, etc. come into contact with copper, copper ions destroy the cell membranes of bacteria, viruses, etc., and cause them to die through disruption of metabolism. .

Zinc (Zn) has an antibacterial function, and the antibacterial principle of zinc can be explained similarly to that of copper.

Brass (CuZn), an alloy of copper and zinc, has an antibacterial function, and the higher the copper content, the higher the antimicrobial activity.

Silver (Ag) has a function of sterilizing or inactivating microorganisms, and in particular, since the number of resistant microorganisms is small compared to antibiotics, disinfectants, etc., silver (Ag) and silver (Ag) compounds can be used in hospital treatment methods.

As the metal layer, for example, an Ag nano wire film or an Ag nano particle film deposited on the substrate 100 in a nano-sized wire shape by sputtering may be used. .

Palladium (Pd) is not oxidized at room temperature and has a characteristic of being oxidized at a high temperature of 800 degrees or more. Palladium can act as a catalyst to reduce metal ions of the metal layer, which are ionized in the antibacterial process, to metal. For example, palladium can reduce copper ions to copper.

Platinum (Pt) has very low chemical reactivity and is not easily oxidized in air, and can act as a catalyst to reduce metal ions of the metal layer ionized in an antibacterial process to metal.

Gold (Au) has very low chemical reactivity and is not easily oxidized in the air, and can act as a catalyst to reduce metal ions of the metal layer, which are ionized in an antibacterial process, to metal.

As such, when the antibacterial layer 300 provided on the substrate 100 comes into contact with foreign harmful substances, it is possible to kill or inactivate foreign harmful substances such as viruses and bacteria.

On the other hand, the carbon layer 400, as shown in (b) of FIG. 2 and (b) of FIG. 4, may be provided by a physical vapor deposition method between the one surface or the other surface of the substrate 100 and the antibacterial layer 300. Here, the carbon layer 400 may be, for example, a graphite or graphene layer, but is not limited thereto.

The physical vapor deposition method may be, for example, a sputtering method. In the process of preparing the carbon layer 400 on the substrate 100 through the sputtering method, a solid carbon target, that is, a bulk carbon target may be used as a sputtering target (not shown). The deposition rate, deposition shape, and uniformity of the carbon layer 400 deposited on the substrate 100 through the sputtering method depend on the voltage applied to the sputtering target, the degree of vacuum in the sputtering chamber (not shown), and adjacent to the sputtering target. It can be adjusted by adjusting the shape of the provided magnet array (not shown) or the strength of the magnetic field.

As such, in the process of depositing any one or two or more selected from the gibberellin layer 200, the antibacterial layer 300, and the carbon layer 400 on the substrate 100 by applying the sputtering method, the substrate 100 is shown in FIG. 5 A roll-to-roll sputtering device shown as an example may be utilized, but is not limited thereto.

As shown in FIG. 5, the roll-to-roll sputtering device includes a chamber 1, an unwinding roller 2, a winding roller 3, a flexible substrate 4, a deposition main drum 5, a sputtering target 6, and a first tension. A holding part 7 and a second tension holding part 8 may be included. The unwinding roller 2, the winding roller 3, the flexible substrate 4, the deposition main drum 5, the sputtering target 6, the first tension holding unit 7 and the second tension holding unit 8 are It can be placed in the chamber (1). The flexible substrate 4 may be supplied from the unwinding roller 2 and returned to the winding roller 3 via the deposition main drum 5 . The first tension holding unit 7 and the second tension maintaining unit 8 are formed on the flexible substrate 4 in the process of depositing a thin film on the surface of the flexible substrate 4 placed on the deposition main drum 5 by the sputtering target 6 ( The tension of 4) can be kept constant. The deposition main drum 5 may be cooled to maintain a constant temperature of the substrate 4 during the process of depositing a thin film on the surface of the substrate 4 by the sputtering target 6 .

In addition, by using the substrate 100 as the flexible substrate 4 in the roll-to-roll sputtering device and using any one or two or more selected from a gibberellin solid target, a metal solid target, and a solid carbon target as the sputtering target 6 Any one or two or more selected from the gibberellin layer 200, the antibacterial layer 300, and the carbon layer 400 may be provided on the substrate 100 by a sputtering deposition method.

Meanwhile, in the roll-to-roll sputtering device, a sputtering target for depositing a gibberellin layer 200 and an antibacterial layer 300 is provided inside a sputtering chamber, the substrate 100 is provided to be spaced apart from the sputtering target, and then the sputtering chamber A magnetic field may be applied to the base material 100 in the same direction through a magnet array (not shown) provided therein.

For example, the substrate 100 is provided by being attached to the flexible substrate 4 opposite to the sputtering target 6, or the substrate 100 replaces the flexible substrate 4 and the unwinding roller 2 and the winding roller directly (3) may be provided in a winding manner.

When paper is used as the base material 100, the paper can be easily damaged by target particles having high energy beyond a standard value during the sputtering process. Accordingly, the technology disclosed in this specification is a magnet array (not shown) provided inside the sputtering chamber 1 to prevent additional acceleration of target particles scattering when Ar cations collide with the sputtering target 6 or to reduce acceleration. By performing sputtering in a state in which a magnetic field is provided in a direction parallel to the substrate 100 through, the energy of the scattering target particles does not exceed a preset range, thereby preventing the substrate 100 from being damaged by the target particles Provides an effect can do.

Hereinafter, experimental details on seed germination using the antibacterial paper for breaking dormancy for plant seed germination assay described above will be described with reference to FIG. 6 .

1. Test method of antibacterial paper for breaking dormancy for plant seed germination test

First, untreated agar medium or filter paper and paper containing gibberellin are immersed in distilled water for 24 hours, and then seeds are plated three times at the optimal germination temperature condition (20 to 25 ° C). At this time, two kinds of seeds with low germination rate were used, Guanampul seed and Mansam seed.

2. Results and discussion

- Guanampul seeds: 10 grains in total, 3 repetitions

* Control group (untreated): 0% germination rate / Treatment group: approximately 70% germination rate

* 55% improvement compared to untreated, 10% reduction compared to optimal GA content

- Mansam seeds: 10 grains in total, 3 repetitions

* Control group (untreated): Germination rate 10% / Treatment group: Germination rate approximately 68.8%

* 57% improvement compared to untreated, 22% reduction compared to optimal GA content

As such, the germination rate is slightly lower than that of the optimal gibberellin condition (treatment by ppm concentration), but the efficiency is more than 50% compared to the untreated condition.

It is judged that the gibberellin-containing paper developed through this experiment will contribute to the search for dormant types by simply performing screening on large samples and then exploring detailed conditions.

100: materials
200: gibberellin layer 210: first gibberellin layer
220: second gibberellin layer
300: antibacterial layer 310: first antibacterial layer
320: second antibacterial layer
400: carbon layer

Claims (6)

With the substrate 100;
a first gibberellin layer 210 provided on one side of the substrate 100 by physical vapor deposition to induce germination of seeds by inducing dormancy breaking of plant seeds;
a first antibacterial layer 310 provided on the substrate 100 or the first gibberellin layer 210 by a physical vapor deposition method;
a second gibberellin layer 220 provided on the other surface of the base material 100 by physical vapor deposition to induce germination of seeds by breaking dormancy of plant seeds;
A second antibacterial layer 320 provided on the other surface of the substrate 100 or the second gibberellin layer 220 by a physical vapor deposition method;
The physical vapor deposition method is deposited by a sputtering device, and the sputtering device includes a sputtering target for depositing a gibberellin layer and an antibacterial layer in a sputtering chamber, and after providing the substrate to be spaced apart from the sputtering target, the inside of the sputtering chamber Antibacterial paper for breaking dormancy for plant seed germination assay, characterized in that sputtering is performed in a state in which a magnetic field is provided in the same direction to the substrate through a magnet array provided in the.
delete According to claim 1,
The substrate 100 is antibacterial paper for breaking dormancy for plant seed germination assay, characterized in that any one of paper, non-woven fabric, fiber, film, and biodegradable plastic is selected and used.
According to claim 1,
The first antibacterial layer 310 and the second antibacterial layer 320 are for plant seed germination assay, characterized in that comprising any one or two or more alloys selected from copper, zinc, palladium, platinum, gold and silver Antibacterial paper for breaking dormancy.
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