KR102482588B1 - Portable Colorimetric Bio-Sensing Device - Google Patents

Abstract

The present invention relates to a portable colorimetric bio sensing device. The portable colorimetric bio sensing device according to the present invention is characterized by comprising: a first reaction chamber in which a first substance is provided to firstly react with an injected specimen; a second reaction chamber in which a second substance is provided to react with the first reactant formed after the specimen and the first substance reacted; an opening and closing valve which is provided to selectively open and close the interval between the first and second reaction chambers; and a detection unit which accommodates a second reactant to check the color of the second reactant which is formed after the first reactant and the second substance reacted. Therefore, provided is the bio sensing device, which can quickly detect harmful substances, can be conveniently carried, and can facilitate ease in maintenance and management.

Description

Portable colorimetric bio-sensing device {Portable Colorimetric Bio-Sensing Device}

The present invention relates to a portable colorimetric biosensing device using a colorimetric reaction proposed to conveniently and quickly detect various harmful substances in various fields, preferably using a colorimetric reaction that is convenient to carry and easy to maintain. It relates to a portable colorimetric bio-sensing device.

As the basic standard of living has improved due to the development of science and technology, mankind has moved beyond one-dimensional needs such as food and shelter to place more importance on issues related to quality of life such as health and the environment. For this reason, interest in harmful substances that impair the quality of life is increasing throughout the fields of food, medicine, housing, industrial sites, and defense.

Air quality is an important factor in residential or industrial sites, and gas can be sampled immediately on site due to its nature, so it is possible to accurately evaluate hazardous substances. Although it is important to secure reliability and accuracy due to high consumer interest, it is difficult to detect harmful substances immediately due to the limited amount of samples and difficulty in collecting accurate samples.

That is, in the case of food or medicine, in most cases, samples are taken from various sites and then delivered to a laboratory to be tested using expensive equipment, resulting in economic and time loss.

For example, in the past, polymerase chain reaction, enzyme immunoassay, ATP bioluminescence, etc. have been used for rapid food safety diagnosis. As a result, the detection period was prolonged. In addition, in the case of other methods, the accuracy of the diagnostic technique and the degree of commercialization are insufficient, so there is a limit that is not realistic.

Meanwhile, the applicant of the present invention published in an academic publication "Development of a portable lab-on-a-valve device for making primary diagnoses based on goldnanoparticle aggregation induced by a switchable linker" (published on October 2020, The Royal Society of Chemistry, 2020) has been published. The above publication introduces a method for rapidly and accurately detecting various harmful substances using a colorimetric reaction by aggregation of gold nanoparticles.

However, in order to quickly and accurately detect harmful substances along with sample collection in the field, development of a hazardous substance detection device that can be used simply and is convenient to carry should be carried out in parallel, but a realistic detection device for effective colorimetric reaction is still proposed It has never happened.

Recently, a detection kit for the purpose of a colorimetric reaction has been proposed, but as it is composed of a number of parts, it is difficult to assemble and install, is inconvenient to carry, and is difficult to access by the general public, so there are limitations in popularization.

The present invention has been devised to solve the above problems, and is convenient to carry, so it is possible to quickly detect harmful substances regardless of location, the user can immediately check the detection result with the naked eye, and additional assembly or disassembly is required. An object of the present invention is to provide a portable colorimetric bio-sensing device that is integrated to be minimized, convenient to use, and easy to maintain so that it can be used multiple times.

In order to achieve the above object, a portable colorimetric bio-sensing device according to an embodiment of the present invention relates to a portable colorimetric bio-sensing device having a kit body 100. A first reaction chamber 10 in which materials are provided; a secondary reaction chamber 20 equipped with a second material for reacting with a primary reactant formed by the reaction of the test substance and the first material; an on/off valve 30 for selectively opening and closing between the first and second reaction chambers 10 and 20; The other side of the secondary reaction chamber 20 has a filter unit 40 coupled to be replaceable to filter a secondary reactant formed by reacting the primary reactant and the second material; and a detection unit 50 accommodating a secondary reactant so that the color of the secondary reactant formed by the reaction between the first reactant and the second material is confirmed, but the kit body 100 has one side into which the test substance is injected. It is characterized in that the primary reaction chamber 10, the opening/closing valve 30, and the secondary reaction chamber 20 are sequentially formed to enable continuous movement of materials to the other side.

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In addition, the valve body 31 of the opening/closing valve 30 is connected to the lever 200 exposed to the outside of the kit body 100, and as the lever 200 rotates, the first and second reaction chambers 10 ) (20) can be selectively opened and closed.

In addition, the kit body 100 is composed of a pair of halves 100a and 100b that can be separated and fastened to each other, and the pair of halves 100a and 100b have a primary reaction chamber groove from one side to the other ( 110a) (110b), the opening/closing valve grooves 130a and 130b, and the secondary reaction chamber grooves 120a and 120b may be sequentially formed.

In addition, the detection unit 50 provided on the other side of the secondary reaction chamber 20 may be provided so that the transparent container 51 is exposed to the outside of the kit body 100 .

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In addition, the filtering unit 40 may be integrally formed with an inlet pipe 43 and an outlet pipe 44 having a pipe shape formed on one side and the other side of the filter body 42 having the filter paper 41 as the center.

According to the portable colorimetric bio-sensing device of the present invention, a first reaction chamber, an on/off valve, and a second reaction chamber are continuously arranged as parts for realizing a colorimetric reaction, so that the test object and the first and second materials are sequentially connected without temporal interruption. , so it has the effect of promptly detecting harmful substances.

In addition, it is possible to expect high-accuracy detection results without errors by fundamentally blocking the possibility of inflow of external foreign substances that may impede the accuracy of detection results.

Furthermore, since most of the parts are integrally provided in one kit body, it is convenient to carry, so there is an advantage in that it is possible to quickly detect harmful substances regardless of location, and it is convenient to use by minimizing additional assembly or disassembly.

In addition, the detection unit is provided to check the color of the filtrate, so that the user can immediately check the detection result with the naked eye.

In addition, by making the filtering unit and the detecting unit selectively separable from the kit body, maintenance is easy and can be used multiple times, and only the filtering unit or the detecting unit, which are difficult to recycle, can be replaced.

1A and 1B are perspective views illustrating the overall shape of a portable colorimetric bio-sensing device according to an embodiment of the present invention.
2a and 2b are disassembled perspective views of a portable colorimetric bio-sensing device according to an embodiment of the present invention;
3 is a cross-sectional view showing the overall shape of a portable colorimetric bio-sensing device according to an embodiment of the present invention.
Figure 4 is a cross-sectional view showing an open state and (b) closed state of the on-off valve according to an embodiment of the present invention (a).
5 is a conceptual diagram for explaining a colorimetric reaction by aggregation of gold nanoparticles according to an embodiment of the present invention.
6 is a block diagram illustrating a hazardous substance sensing method in time series according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in more detail with reference to the accompanying drawings. The embodiments of the present invention can be modified in various forms, and the scope of the present invention should not be construed as being limited to the following examples. This embodiment is provided to more completely explain the present invention to those skilled in the art.

The portable colorimetric bio-sensing device according to the present invention injects an object collected from a sample, first reacts with a first material to form a first reactant, and additionally reacts the first reactant with a second material to form a second reactant. After forming, it is proposed to check the detection result according to the colorimetric reaction, and it is preferable to manufacture the first material and the second material to be provided in the sensing device SD in advance.

In addition, the portable colorimetric bio-sensing device (SD) according to the present invention is manufactured to be easily carried and stored in an integrated state by the kit body 100 as a whole, as shown in the perspective view of the coupled state of FIGS. 1A and 1B. As shown in the cross-sectional view of FIG. 3, the first reaction chamber 10, the opening/closing valve 30, and the second reaction chamber 20 may be sequentially formed in the kid body 100.

The first reaction chamber 10 is a space portion in which a first material is provided, and at the same time also functions as a space portion for a primary reaction with an object collected and injected from a sample. An inlet 11 may be formed at one side of the primary reaction chamber 10 so that a test object may be injected, and the inlet may be selectively opened by a cap 12 . The cap 12 may be detachably provided in various ways capable of opening and closing the inlet 11 . In addition, the inlet 11 may be formed on the top or side of the kit body 100, unlike the shape shown in the drawings.

On the other hand, the second reaction chamber 20 is a space where the second material is provided, and at the same time, the first reactant formed by the first reaction between the first material and the test object in the first reaction chamber 10 is It also functions as a space for secondary reactions with secondary substances. The secondary reaction chamber 20 is formed on the other side of the primary reaction chamber 10, and between the primary reaction chamber 10 and the secondary reaction chamber 20, the primary reactant is disposed in the secondary reaction chamber ( 20) is provided with an opening/closing valve 30 for opening and closing the space between them to selectively control the movement.

The opening/closing valve 30 may be manufactured in various ways capable of opening and closing a space between the first reaction chamber 10 and the second reaction chamber 20 .

In one embodiment, the on-off valve 30 is provided so that the valve body 31 through which the cylinder hole 31a is formed is rotatable, and according to the rotation of the valve body 31, the first and second reaction chambers 10 ) (20) may be formed to selectively communicate. In another embodiment, although not shown in the drawings, the on-off valve 30 is manufactured in a rod-shaped structure that can move up and down so that the space between the first and second reaction chambers 10 and 20 can be opened and closed. can also be manufactured.

Meanwhile, a filtering unit 40 may be provided to filter a secondary reactant formed by reacting the primary reactant and the second material in the secondary reaction chamber 20 . The filtering unit 40 may be integrally formed with a filter body 42 having a filter paper 41. The filter paper 41 may be manufactured in various types according to the nature of the secondary reactant to be filtered, and the pore size may also be selectively adopted.

The filter body 42 of the filtration unit 40 is integrally formed with an inlet pipe 43 in the direction of the secondary reaction chamber 20 into which the secondary reactant flows, and the filtrate passing through the filter paper 41 The discharge pipe 44 is integrally formed in the direction of the detection unit 50, which will be described later, through which the discharge pipe 44 is discharged, so that it can perform a guide function so that it can move naturally even when the pressure changes rapidly.

On the other hand, the detection unit 50 is to accommodate it so that the color of the secondary reactant or filtrate is confirmed, the detection unit 50 may be made of a transparent container (51).

According to the portable colorimetric bio-sensing device (SD) of the present invention described above, the first reaction chamber 10, the on-off valve 30 and the second reaction chamber 20 are provided on one side as parts for implementing a colorimetric reaction. Since the test object and the first and second substances can react sequentially without temporal interruption by being continuously disposed on the side, there is an effect of rapidly detecting harmful substances.

On the other hand, as shown in FIG. 3, the present invention is such that the kit body 100 is formed as described above, and the material is continuously moved from one side to which the test object is injected into the kit body 100 to the other side. The first reaction chamber 10, the opening/closing valve 30, and the second reaction chamber 20 may be sequentially formed.

The kit body 100 is preferably formed to entirely cover the detailed parts of the portable colorimetric bio-sensing device SD, except for the detection unit 50 that needs to be visually observed from the outside, the first reaction chamber 10 ), the opening/closing valve 30 and the secondary reaction chamber 20 can be formed inside the kit body 100, thereby fundamentally blocking the possibility of inflow of external foreign substances that can impede the accuracy of the detection result, resulting in a high level without error. Accurate detection results can be expected.

In addition, since most of the parts are integrally provided in the kit body 100, it is convenient to carry and can quickly detect harmful substances regardless of location, and it is convenient to use by minimizing additional assembly or disassembly.

In one embodiment, as shown in FIGS. 2A and 2B, the kit body 100 is composed of a pair of halves 100a and 100b capable of being separated and fastened to each other, and the pair of halves 100a ( In 100b), primary reaction chamber grooves 110a and 110b, opening/closing valve grooves 130a and 130b, and secondary reaction chamber grooves 120a and 120b may be sequentially formed from one side to the other.

That is, when the pair of halves 100a and 100b are detachably provided, the convenience of maintenance can be secured, and the primary reaction chamber groove portion ( 110a and 110b form the first reaction chamber 10, and the second reaction chamber grooves 120a and 120b may form the second reaction chamber 20. However, a separate endothelium may be additionally provided in the groove portion to form the first and second reaction chambers 10 and 20.

Also, depending on the embodiment, the filtering unit 40 may be formed integrally with the filter body 42 having the filter paper 41 so that the filtering unit 40 is detachable. As a result, since the function of the filtering unit 40 deteriorates due to repeated use and requires periodic replacement, the filtering unit 40 is manufactured to have a separate filter body 42 so that the filtering unit 40 whose life has expired can be replaced. it is desirable

Meanwhile, it is preferable that the detector 50 is formed of a transparent container 51 and exposed to the outside of the kit body 100 so that the color of the filtrate can be confirmed with the naked eye. That is, since the detection unit 50 also requires periodic replacement, it is possible to replace the detection unit 50 whose life has expired.

Depending on the embodiment, the transparent container may be screwed to the fastening head 52, and the outlet 44 of the filtering unit 40 may pass through the inside of the fastening head 52, or the outlet 44 By forming a stopper 44a on the outer circumferential surface of the stopper 44a so as not to be separated from the fastening head 52, a tight coupling structure between the filtering unit 40 and the detection unit 50 can be expected.

In addition, the filtering unit 40 and the detecting unit 50 may be formed to be separated and coupled at the same time, and for this purpose, the filtering unit 40 and the detecting unit 50 may be manufactured as an integrated structure.

On the other hand, as described above, the on-off valve 30 is provided so that the valve body 31 is rotatable, and in the embodiment in which the cylinder hole 31a is formed through the valve body 31, as shown in FIG. Likewise, the lever 200 is connected to the valve body 31, and the lever 200 is formed to be exposed to the outside of the kit body 100, so that the first and second reaction chambers are formed according to the rotation of the lever 200. (10) It can be manufactured to be selectively opened and closed between (20).

Figure 4 (a) shows a state in which the valve body 31 opens between the first and second reaction chambers 10 and 20, and Figure 4 (b) shows the valve body 31 is 1 It shows a closed state between the primary and secondary reaction chambers 10 and 20.

On the other hand, the valve body 31 is preferably formed to have a round surface to ensure easy rotation, and between the first reaction chamber 10 and the second reaction chamber 20 formed on one side and the other side, respectively Each support flange 32 is provided to form a tight structure so that the rotation of the valve body 31 does not leak.

To this end, it is preferable that the support flange 32 is also manufactured in a shape corresponding to the round surface of the valve body 31 . In addition, although the support flange 32 may be made of an elastic material, it is preferable to additionally include a sealing body made of an elastic material to prevent leakage.

Hereinafter, as a representative example, the first substance is a 'converting functional linker', the second material is a 'gold nanoparticle', and the harmful substance to be detected is 'Salmonella (antigen)', based on the present invention. The operating principle of the bio-sensing device (SD) using a colorimetric reaction will be described.

The conversion functional linker is a linker that induces aggregation by selectively binding harmful substances, and the gold nanoparticles have aggregation ability depending on the degree of harmful substances bound to the conversion functional linker while aggregation is induced by the conversion functional linker. There are characteristics that are affected.

Figure 5 (a) is a schematic diagram showing the shape of a linker with a conversion function, a structure in which biotin is coupled to an antibody, and as shown in the upper right corner, streptavidin equipped with the biotin in gold nanoparticles ( Aggregation of gold nanoparticles can be induced through reaction with streptavidin, but, as shown in the bottom right, when an antigen is present, an antigen-antibody reaction occurs, so there is a limitation in inducing aggregation of gold nanoparticles will do

Figure 5 (b) is a schematic diagram showing the reaction of the control group (Control) and the test group (Target) that can be generated by the conversion function linker. The control group on the left shows a state in which aggregation of gold nanoparticles is induced by the conversion function linker forming a biotin-strapavidin reaction, and in the test group on the right, the conversion function linker forms an antigen-antibody reaction, thereby showing a state in which aggregation of gold nanoparticles is induced. It shows a state in which aggregation of is limitedly induced.

If this principle is applied to the bio-sensing device (SD) of the present invention, the first material, the conversion function linker, is provided in the first reaction chamber 10, and the second material, gold nanoparticles, is provided in the second reaction chamber 20 ) is provided. Since the initial opening/closing valve 30 is in a closed state, the conversion function linker and the gold nanoparticles are stored in a separated state without mutual reaction.

Thereafter, the cap 12 is covered in a state in which the specimen is injected into the primary reaction chamber 10 equipped with a conversion function linker through the injection port 11, and a predetermined flow (stirring) is applied to the primary reaction chamber ( In 10), a primary reaction occurs, and the primary reactant formed as a result is moved to the secondary reaction chamber 20 together with the opening/closing valve 30.

On the other hand, when the first reactant completely moves into the second reaction chamber 20, by applying a predetermined flow (agitation) with the on-off valve 30 closed again, the second reaction chamber 20 is equipped with A secondary reactant is formed through a secondary reaction with gold nanoparticles.

Depending on the embodiment, the opening/closing valve 30 may be opened later to induce movement by applying a predetermined pressure so that the secondary reactant can pass through the filter paper 41 of the filtering unit 40. A syringe or the like may be coupled to the inlet 11 to provide pressure. The filtrate passing through the filtering unit 40 is accommodated in the transparent container 51 of the detecting unit 50 .

At this time, when there is no antigen in the specimen or the antigen is present below the standard value, aggregation of the gold nanoparticles is induced and the color becomes blue, and when the antigen is present at or above the standard value, the aggregation of the gold nanoparticles is limitedly induced, resulting in aggregation The gold nanoparticles appear red in color. As a result, it is possible to easily observe with the naked eye whether or not harmful substances are present in the collected specimen, so that it is possible to quickly and accurately detect harmful substances in the field.

Hereinafter, the harmful substance sensing method (M) using the portable colorimetric bio-sensing device (SD) of the present invention will be described in detail. 6 is a block diagram showing a method M for sensing harmful substances in time series according to an embodiment of the present invention.

First, a test object is injected into the primary reaction chamber 10 equipped with a first material (S10). Since an inlet 11 communicating to the outside is formed on one side of the primary reaction chamber 10, the collected specimen can be injected. The specimen may be injected into the first reaction chamber 10 using various known injection means.

Thereafter, a first reactant is formed by reacting the first material with the test object in the first reaction chamber 10 (S20). Since the initial opening/closing valve 30 is closed, it is preferable to proceed with the first reaction by applying a predetermined flow while covering the cap 12, which is selectively detachable to the outside of the inlet 11.

When the first reactant is completed, the open/close valve 30 is opened to move the first reactant to the second reaction chamber 20 equipped with the second material (S30). At this time, a predetermined slope (gradient) may be formed between the first reaction chamber 10 and the second reaction chamber 20 so that the first reactant can move naturally. It is also possible to implement the primary reactant to naturally move along the slope by binding to the body 100 .

Thereafter, the second material and the first reactant are reacted by closing the opening/closing valve 30 to form a second reactant (S40). Likewise, it is preferable to proceed with the secondary reaction by applying a predetermined flow.

Depending on the embodiment, when the secondary reactant is completed, the secondary reactant may be filtered through the filtration unit 40 (S50). The step S50 may include opening the on/off valve 30 (S51) and forming a pressure in one direction so that the secondary reactant passes through the filtration unit 40 (S52).

That is, it is preferable to induce the movement by applying a predetermined pressure so that the secondary reactant can pass through the filter paper 41 of the filtration unit 40, and in one embodiment, by coupling a syringe or the like to the inlet 11 pressure can be provided.

Thereafter, the color of the filtrate or the secondary reactant received in the detection unit 50 after passing through the filtration unit 40 is checked (S60). Thus, according to the harmful substance sensing method (M) using the bio-sensing device of the present invention, it is possible to quickly and accurately detect the level of harmful substances contained in the test object in the field.

In the above, the portable colorimetric bio-sensing device (SD) of the present invention and the harmful substance sensing method (M) using the same have been mainly described and described, but those of ordinary skill in the art have described the present invention described in the claims The present invention can be variously modified and changed by adding, changing, deleting or adding elements within the scope of not departing from the spirit of the above, and this will also be said to be included in the scope of the present invention.

Furthermore, in describing the embodiments of the present invention, if it is determined that a detailed description of a known function or configuration may unnecessarily obscure the subject matter of the present invention, the detailed description is omitted. In addition, the terms used are terms defined in consideration of functions in the embodiments of the present invention, which may vary according to the intention or relationship of the user or operator. Therefore, the definition should be made based on the contents throughout this specification. Accordingly, the above detailed description of the invention is not intended to limit the invention to the disclosed embodiments, and the appended claims should be construed to include other embodiments as well.

SD: bio-sensing device 100: kit body
10: primary reaction chamber 11: inlet
12: cap 20: secondary reaction chamber
30: on-off valve 31: valve body
31a: cylinder hole 32: support flange
40: filter unit 41: filter paper
42: filter body 43: inlet pipe
44: discharge pipe 50: detection unit
51: transparent container 52: fastening head
200: lever
M: Hazardous Substance Sensing Method

Claims (7)

It relates to a portable colorimetric bio-sensing device having a kit body 100,
a primary reaction chamber 10 equipped with a first material for primary reaction with the injected test object;
a secondary reaction chamber 20 equipped with a second material for reacting with a primary reactant formed by the reaction of the test substance and the first material;
an on/off valve 30 for selectively opening and closing between the first and second reaction chambers 10 and 20;
The other side of the secondary reaction chamber 20 has a filter unit 40 coupled to be replaceable to filter a secondary reactant formed by reacting the primary reactant and the second material; and
A detection unit 50 accommodating the secondary reactant so that the color of the secondary reactant formed by the reaction of the first reactant and the second material is confirmed;
In the kit body 100, the first reaction chamber 10, the opening/closing valve 30, and the second reaction chamber 20 are sequentially formed to enable continuous movement of substances from one side into which the test object is injected to the other side. Portable colorimetric bio-sensing device, characterized in that being.
delete According to claim 1,
The valve body 31 of the opening/closing valve 30 is connected to the lever 200 exposed to the outside of the kit body 100, and as the lever 200 rotates, the first and second reaction chambers 10 ( 20) A portable colorimetric bio-sensing device characterized in that the gap is selectively opened and closed.
According to claim 1,
The kit body 100 is composed of a pair of halves 100a and 100b that can be separated and fastened to each other, and the pair of halves 100a and 100b have primary reaction chamber grooves 110a from one side to the other. (110b), the opening/closing valve grooves (130a, 130b), and the secondary reaction chamber grooves (120a, 120b) are sequentially formed.
According to claim 1,
The detection unit 50 provided on the other side of the secondary reaction chamber 20 is a portable colorimetric bio-sensing device, characterized in that provided so that the transparent container 51 is exposed to the outside of the kit body 100.
delete According to claim 1,
The filtering unit 40 is characterized in that the inlet pipe 43 and the discharge pipe 44 are formed integrally with one side and the other side of the filter body 42 having the filter paper 41 as the center. Portable colorimetric bio-sensing device.

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