KR102445766B1 - Oil-in-water emulsion composition and food and beverage containing same - Google Patents

Abstract

Contains astaxanthin, oily component, enzyme-decomposed lecithin, sucrose fatty acid ester, water-soluble tea extract, and water, and the water-soluble tea extract contains 20% by mass or more of tea polyphenols based on the total mass of the water-soluble tea extract, Oil-in-water emulsification, wherein the content rate of astaxanthin with respect to the total mass of the oil-in-water emulsion composition is 0.1 mass % to 20.0 mass %, and the content rate of the water-soluble tea extract with respect to the total mass of the oil-in-water emulsion composition exceeds 0 mass % and 20.0 mass % or less Compositions and their applications.

Description

Oil-in-water emulsion composition and food and beverage containing same

The present disclosure relates to an oil-in-water emulsion composition and food and beverage containing the same.

Astaxanthin is attracting attention as a component having an "antioxidant action" that eliminates active oxygen, and in recent years has been widely distributed as a raw material for health food. Astaxanthin is a kind of natural carotenoid pigment, and is widely distributed in the natural world, and is contained, for example, in fish such as salmon and red sea bream, crustaceans such as crab, shrimp and krill, algae, and the like.

However, when astaxanthin is used as a food raw material, it has poor digestibility and absorption due to its low solubility in water, and also has drawbacks such as a decrease in the palatability of a product due to a peculiar smell derived from the raw material.

Japanese Patent Publication No. 4163218 discloses an emulsion composition containing hematococcus algae pigment that is stable, does not generate odor, and can be stored for a long time, suitable for use in various foods, for the purpose of, An emulsion composition comprising an oil containing a coccus algal pigment, at least an emulsifier in an amount sufficient to emulsify the oil, a tea extract, and a hydrophilic medium is prepared. A method for suppressing a characteristic odor is disclosed.

The present invention produced an emulsion for the purpose of an astaxanthin-containing oil-in-water emulsion composition for food additives with high palatability, which reduced the unique odor derived from the raw material of astaxanthin, the method described in Japanese Patent Publication No. 4163218 It turned out that emulsion stability worsened.

One of the embodiments of the present invention makes it a subject to provide an oil-in-water emulsion composition which suppresses the odor derived from the raw material of astaxanthin in view of the above problems and is excellent in emulsion stability.

One of the embodiments of the present invention makes it a subject to provide a food or beverage containing an oil-in-water emulsion composition which suppresses the odor derived from the raw material of astaxanthin and is excellent in emulsion stability.

The present inventors found that astaxanthin, oily components, enzymatically digested lecithin, sucrose fatty acid ester, water-soluble tea extract, and water are included, and the water-soluble tea extract contains 20% by mass or more of tea polyphenols based on the total mass of the water-soluble tea extract. containing, the content rate of astaxanthin with respect to the total mass of the oil-in-water emulsion composition is 0.1 mass% to 20.0 mass%, and the content rate of the water-soluble tea extract with respect to the total mass of the oil-in-water emulsion composition is more than 0 mass% and 20.0 mass% or less, The oil-in-water emulsion composition discovered that the said subject could be solved.

Specific means for solving the above problems include the following embodiments.

[1] Contains astaxanthin, oily component, enzyme-decomposed lecithin, sucrose fatty acid ester, water-soluble tea extract, and water, and the water-soluble tea extract contains 20% by mass or more of tea polyphenols based on the total mass of the water-soluble tea extract And, the content rate of astaxanthin with respect to the total mass of the oil-in-water emulsion composition is 0.1 mass% to 20.0 mass%, and the content rate of the water-soluble tea extract with respect to the total mass of the oil-in-water emulsion composition is more than 0 mass% and 20.0 mass% or less, Oil-in-water emulsion composition.

[2] The oil-in-water emulsion composition according to [1], further comprising a polyglycerol fatty acid ester.

[3] The oil-in-water emulsion composition according to [1], wherein the emulsifier contains at least one selected from the group consisting of polyglycerol fatty acid esters, sucrose fatty acid esters and enzymatically digested lecithin.

[4] The oil-in-water according to [2] or [3], which contains a polyglycerol fatty acid ester, and the content of the polyglycerol fatty acid ester with respect to the total mass of the oil-in-water emulsion composition is in the range of 2% by mass or more and 22% by mass or less. Emulsifying composition.

[5] The oil-in-water emulsion composition according to any one of [1] to [4], wherein the water-soluble tea extract is contained in the aqueous phase.

[6] The oil-in-water emulsion composition according to any one of [1] to [5], wherein the sucrose fatty acid ester has a ratio of mono-substituted products to the total mass of the sucrose fatty acid ester of 75% by mass or more.

[7] The oil-in-water emulsion composition according to any one of [1] to [6], wherein the sucrose fatty acid ester is contained in an amount of 0.01 mass % or more and 10 mass % or less with respect to the total mass of the oil-in-water emulsion composition.

[8] The oil-in-water emulsion composition according to any one of [1] to [7], wherein the enzyme-decomposed lecithin is contained in an amount of 0.00001 mass % or more and 7 mass % or less with respect to the total mass of the oil-in-water emulsion composition.

[9] The oil-in-water emulsion composition according to any one of [1] to [8], further comprising glycerin in an amount of 20% by mass or more and 50% by mass or less with respect to the total mass of the oil-in-water emulsion composition.

[10] The oil-in-water emulsion composition according to any one of [1] to [9], wherein the water-soluble tea extract contains 80% by mass or more of tea polyphenols based on the total mass of the water-soluble tea extract.

[11] The oil-in-water emulsion composition according to any one of [1] to [10], wherein the oily component is at least one selected from the group consisting of medium-chain fatty acid triglycerides and animal and vegetable oils.

[12] The oil-in-water emulsion composition according to any one of [1] to [11], which is used as a food additive.

[13] A food comprising the oil-in-water emulsion composition according to any one of [1] to [12].

[14] A beverage comprising the oil-in-water emulsion composition according to any one of [1] to [12].

According to one of the embodiments of the present invention, it is possible to provide an astaxanthin-containing oil-in-water emulsion composition which suppresses the odor derived from the raw material of astaxanthin and is excellent in emulsion stability.

Further, according to one of the embodiments of the present invention, it is possible to provide a food or beverage containing an astaxanthin-containing oil-in-water emulsion composition which suppresses the odor derived from the raw material of astaxanthin and is excellent in emulsion stability.

EMBODIMENT OF THE INVENTION Hereinafter, although specific embodiment of this invention is described in detail, this invention is not limited to the following embodiment, Within the range of the objective of this invention, it can implement by adding a change suitably.

In the present disclosure, the numerical range indicated using “to” means a range including the numerical values before and after “to” as the minimum and maximum values, respectively.

In the numerical range described in steps in the present disclosure, the upper limit or lower limit described in a certain numerical range may be substituted with the upper limit or lower limit of the numerical range described in another stepwise manner. In addition, in the numerical range described in this indication, you may substitute the value shown in the Example for the upper limit or lower limit described in a certain numerical range.

In the present disclosure, the amount of each component means the total amount of the plurality of types of substances present in the composition, unless otherwise specified, when a plurality of types of substances corresponding to each component exist in the composition.

In this indication, the combination of two or more preferable aspects is a more preferable aspect.

In the present disclosure, "HLB (Hydrophile-Lipophile Balance) value" uses a commercially available product as a component, and in literature, such as a catalog of a commercial product, when the HLB value of the commercial product is clearly indicated, literature, such as a catalog Use the values shown in

When the component to be used is not a commercial item, or when an HLB value is not clearly shown in literature, such as a catalog, even if it is commercially available, the Kawakami formula shown below is employ|adopted as an HLB value in this indication.

HLB=7+11.7log(M _w /M ₀ )

Here, M _W is the molecular weight of the hydrophilic group, and M ₀ is the molecular weight of the hydrophobic group.

In the present disclosure, the term "process" is included in this term as long as the intended purpose of the process is achieved, even if it is not an independent process and cannot be clearly distinguished from other processes.

In the present disclosure, "aqueous phase" is used as a word for "oil phase" regardless of the type of solvent.

One of the embodiments of the present disclosure includes astaxanthin, an oily component, enzymatically digested lecithin, sucrose fatty acid ester, a water-soluble tea extract, and water, wherein the water-soluble tea extract contains tea polyphenols based on the total mass of the water-soluble tea extract contains 20% by mass or more, the content rate of astaxanthin with respect to the total mass of the oil-in-water emulsion composition is 0.1 mass% to 20.0 mass%, and the content rate of the water-soluble tea extract with respect to the total mass of the oil-in-water emulsion composition is 0 mass% It is an oil-in-water emulsion composition (hereinafter, simply referred to as "oil-in-water emulsion composition of the present disclosure" in some cases) exceeding 20.0 mass % or less.

One of the embodiments of the present disclosure is a food or beverage (hereinafter, simply referred to as “food of the present disclosure” or “beverage of the present disclosure” in some cases) comprising the oil-in-water emulsion composition of the present disclosure.

[Oil-in-water emulsion composition]

The oil-in-water emulsion composition of the present disclosure contains astaxanthin, an oily component, an enzymatically decomposed lecithin, a sucrose fatty acid ester, and an oil-in-water emulsion in a content ratio of 0.1% by mass to 20.0% by mass relative to the total mass of the oil-in-water emulsion composition. It is an oil-in-water emulsion composition comprising water and a water-soluble tea extract having a content of more than 0% by mass and not more than 20.0% with respect to the total mass of the composition and containing 20% by mass or more of tea polyphenols based on the total mass of the water-soluble tea extract.

Astaxanthin is attracting attention as a component having an "antioxidant action" that eliminates active oxygen. Moreover, it is known that astaxanthin has various functions, such as immune vitality, fatigue recovery, and balance adjustment of an autonomy nerve, and it is used abundantly as a raw material, such as a health food. However, it became clear from examination of this inventor that the palatability of a product may fall by the odor derived from the raw material of astaxanthin. Such a phenomenon tends to occur especially when a food containing astaxanthin at a higher concentration is prepared for the purpose of functional display or the like.

On the other hand, according to the present disclosure, by including a specific amount of astaxanthin, an oily component, an enzyme-decomposed lecithin, a sucrose fatty acid ester, a specific amount of a water-soluble tea extract, and water, odor is suppressed and emulsified An oil-in-water emulsion composition having excellent stability (especially storage stability over time at room temperature) can be provided.

In the present disclosure, room temperature refers to “25±3° C.”.

Regarding this point, the present inventor estimates as follows.

In general, it is known that tea extract has a deodorizing effect. However, according to the study of the present inventor, in order to reduce the odor derived from the raw material of astaxanthin, when tea extract is contained in the oil-in-water emulsion composition, it can be seen that the stability of the oil-in-water emulsion composition deteriorates over time at room temperature. there was. As the present inventors have studied repeatedly, among tea extracts, water-soluble tea extracts containing 20% by mass or more of tea polyphenols are included in a specific amount, and oil-in-water emulsions containing oily components, enzymatically decomposed lecithin, and sucrose fatty acid esters By setting it as a composition, it became clear that the odor derived from the raw material of astaxanthin was suppressed, and exhibited the outstanding emulsion stability, although astaxanthin of high concentration was included.

In addition, by setting the oil-in-water composition of the present disclosure as described above, the stability of the oil-in-water emulsion composition when stored under refrigeration is also excellent, and the viscosity of the oil-in-water emulsion composition by astaxanthin blended at a high concentration. A rise can be suppressed and it is thought that it has formulation aptitude as an oil-in-water emulsion.

In this indication, refrigeration storage refers to the preservation|save in the atmospheric temperature of "more than 1 degreeC and 10 degrees C or less". In addition, in this indication, refrigeration storage and low temperature storage are synonymous.

In addition, said assumption does not interpret the oil-in-water composition of this indication limitedly, but demonstrates it as an example.

[Astaxanthin]

The oil-in-water emulsion composition of the present disclosure contains astaxanthin.

The oil-in-water emulsion composition of the present disclosure contains astaxanthin. In the present disclosure, unless otherwise specified, astaxanthin and its derivatives are collectively referred to as "astaxanthin". That is, in the oil-in-water emulsion composition of the present disclosure, at least one selected from the group consisting of astaxanthin and derivatives thereof is included as astaxanthin. Astaxanthin derivatives include astaxanthin esters and the like.

As the astaxanthin, a synthetic product of astaxanthin obtained by a conventional method other than astaxanthin derived from natural products such as plants, algae, crustaceans, and bacteria can also be used.

Astaxanthin can be extracted from cultures such as red yeast papia, algae hematococcus, marine bacteria, and krill.

From the viewpoint of quality and productivity, astaxanthin is preferably an extract from Haematococcus algae (hereinafter referred to as "Hematococcus algae extract") or astaxanthin derived from an extract from krill, Astaxanthin derived from Matococcus algae extract is particularly preferred.

In the oil-in-water emulsion composition of the present disclosure, it is preferable that astaxanthin is dissolved in an oily component to be described later. That is, astaxanthin can be blended with the oil-in-water emulsion composition of the present disclosure in the form of astaxanthin-containing oil in which astaxanthin is contained in the oily component.

Specific examples of Haematococcus algae include Haematococcus pluvialis, Haematococcus lacustris, Haematococcus capensis, Haematococcus capensis. and Cus droebakensis (Haematococcus droebakensis) and Haematococcus zimbabwiensis (Haematococcus zimbabwiensis).

Among these, as a Haematococcus alga, Haematococcus pluvialis is preferable.

The Hematococcus algae extract is obtained by disrupting the cell wall of the Hematococcus algae, if necessary, by the method disclosed in Japanese Patent Application Laid-Open No. Hei 5-068585, etc., followed by acetone, ether, chloroform, alcohol (ethanol , methanol, etc.) or an extraction solvent such as carbon dioxide in a supercritical state.

As the Haematococcus algae extract, a commercially available product may be used.

Examples of commercial products of the Hematococcus algae extract include Fujifilm Co., Ltd. ASTOTS (registered trademark)-S, ASTOTS (registered trademark)-5O, ASTOTS (registered trademark)-10O, etc., manufactured by Fuji Chemical Co., Ltd. Astaryl (registered trademark) oil 50F, astaryl (registered trademark) oil 5F, etc., BioAstin SCE7 of Toyoko Chemical Co., Ltd., etc. are mentioned.

The content rate of astaxanthin as a pure pigment in the Hematococcus algae extract is, from the viewpoint of handling at the time of production, preferably 0.001 mass % to 50 mass %, more preferably 0.01 mass % to 40 mass % to be.

In addition, the Hematococcus algae extract may contain astaxanthin or its ester form as a pure pigment, similarly to the pigment|dye described in Unexamined-Japanese-Patent No. 2-049091.

The content rate of astaxanthin in the oil-in-water emulsion composition of this indication is 0.1 mass % - 20.0 mass %.

The content rate of astaxanthin is, for example, from the viewpoint of sufficiently obtaining the effect expected by including astaxanthin, with respect to the total mass of the oil-in-water emulsion composition, it is preferably 0.2 mass % or more, and more preferably 0.4 mass % or more. and more preferably 1.0 mass % or more.

Moreover, it is preferable that it is 10 mass % or less with respect to the total mass of an oil-in-water emulsion composition from a viewpoint of easiness of intake, for example, and, as for the content rate of astaxanthin, it is more preferable that it is 8 mass % or less.

[oily ingredients]

The oil-in-water emulsion composition of the present disclosure contains an oily component.

In the oil-in-water emulsion composition of the present disclosure, the oily component is a component having a solubility in water at 25°C of less than 0.1 mass % (less than 1 g/L). The oily component can be suitably used as a compound used for the purpose of dissolving astaxanthin. Astaxanthin is not included in the oily component in the oil-in-water emulsion composition of the present disclosure.

As an oily component, it is preferable to contain at least 1 sort(s) selected from the group which consists of a medium-chain fatty acid triglyceride and animal and vegetable fats and oils from a viewpoint on affinity with astaxanthin and the Food Sanitation Act.

As an example of a medium-chain fatty acid triglyceride, C8-10 medium-chain fatty acid triglyceride of the fatty acid which comprises a triglyceride is mentioned.

Examples of animal and vegetable oils and fats include vegetable oils such as soybean oil, rapeseed oil, cottonseed oil, sunflower oil, safflower oil, palm oil, wheat germ oil, corn germ oil, olive oil, rice bran oil, coconut oil, liver oil, fish oil, etc. , and animal fats such as light oil.

Among these oily components, medium-chain fatty acid triglycerides are preferable, and as the oily component contained in the oil-in-water emulsion composition of the present disclosure, only medium-chain fatty acid triglycerides are more preferable.

As described above, astaxanthin is an astaxanthin-containing oil containing astaxanthin in an oily component, and may be contained in the oil-in-water emulsion composition of the present disclosure, or may be derived from the antioxidant B described later. It is preferable that astaxanthin containing oil contains a medium-chain fatty acid triglyceride as an oily component.

The number of oily components may be one, and they may combine 2 or more types.

It is preferable that it is the range of 15 mass % or more and 50 mass % or less with respect to the total mass of the oil-in-water emulsion composition, and, as for the total content of an oil component, it is more preferable that it is the range of 20 mass % or more and 50 mass % or less.

[Water-soluble tea extract]

The oil-in-water emulsion composition of the present disclosure contains a water-soluble tea extract having a content of more than 0 mass% and 20.0 mass% or less with respect to the total mass of the oil-in-water emulsion composition.

The water-soluble tea extract used in the oil-in-water emulsion composition of the present disclosure contains 20% by mass or more of tea polyphenols based on the total mass of the water-soluble tea extract.

The water-soluble tea extract used in the oil-in-water emulsion composition of the present disclosure is not particularly limited as long as it is a tea extract having a solubility in water at 25°C of 20% by mass or more (200g/L or more).

The water-soluble tea extract is extracted from one or two or more types of tea leaves selected from green tea, oolong tea, and black tea, and contains 20 mass% or more of tea polyphenols (catechins) based on the total mass of the water-soluble tea extract. . The solvent (also called "extraction solvent") used for extraction is not specifically limited, Hot water, methanol, ethanol, isopropanol, ethyl acetate, etc. are mentioned. By performing an extraction operation using one or a mixture of two or more of these extraction solvents, an extract containing a water-soluble tea extract is obtained.

In the case of hot water extraction, the extract can be used as it is, preferably in a powder form from which water has been removed by spray drying, freeze drying, etc.

In the oil-in-water emulsion composition of the present disclosure, the water-soluble tea extract functions as an antioxidant. Hereinafter, the water-soluble tea extract according to the present disclosure is also referred to as "antioxidant A".

From the viewpoint of reducing odor, the lower limit of the content of the antioxidant A is preferably 0.1 mass% or more, more preferably 0.3 mass% or more, based on the total mass of the oil-in-water emulsion composition.

On the other hand, from the viewpoint of reducing the viscosity of the oil-in-water emulsion composition by astaxanthin to improve production aptitude, the upper limit of the content of antioxidant A is not particularly limited, but from the viewpoint of emulsion stability, it is 20.0% by mass or less, Preferably it is 15.0 mass % or less, More preferably, it is 10.0 mass % or less, More preferably, it is 8.0 mass % or less, More preferably, it is 5.0 mass % or less.

As antioxidant A, commercially available products can also be used, Sunfood (registered trademark) 100, CD, aqueous, horse (30%) (above, trade name, manufactured by Mitsubishi Chemical Foods Co., Ltd.), Sanphenone (registered trademark) 30S- OP, EGCG-OP, BG-3, 90S, 90MB-OP (above, trade name, manufactured by Daiyo Chemical Co., Ltd.), polyphenone (registered trademark) G, 70S, PF (above, trade name, Mitsui Norin Co., Ltd.) ), Nteafuran (registered trademark) 30A, 90S (above, brand name, manufactured by Itoen Co., Ltd.), Sanwoolong (registered trademark, brand name, manufactured by Suntory Co., Ltd.), and the like.

Antioxidant A is, from the viewpoint of coexistence of odor reduction and emulsion stability, as tea polyphenols, catechins having a basic structure of flavan-3-ol, that is, epicatechin, epigallocatechin, epicatechin gallate, epigal It is preferable to include one or two or more selected from the group consisting of locatechin gallate, catechin, gallocatechin, catechin gallate, and gallocatechin gallate. From the viewpoint of reducing odor, the content of the tea polyphenol relative to the total mass of the antioxidant A is preferably 40 mass % or more, more preferably 50 mass % or more, still more preferably 70 mass % or more, and 80 mass % or more. % or more is even more preferred. When the oil-in-water emulsion composition of the present disclosure is applied to food or beverage, from the viewpoint of reducing odor, the tea polyphenol contains at least one or more of epigallocatechin gallate or epigallocatechin in 10% by mass or more. it is preferable Moreover, it is preferable that an upper limit is 50 mass % or less from a viewpoint of enabling sufficient odor reduction while suppressing the bitterness peculiar to tea.

In the oil-in-water emulsion composition of the present disclosure, at least one of the aqueous phase and the oil phase may contain the antioxidant A, but from the viewpoint of more effectively suppressing the odor derived from the raw material of astaxanthin, the antioxidant A is contained in the aqueous phase. it is preferable

In this regard, the present inventors believe that when the antioxidant A is contained in the aqueous phase, in the oil-in-water composition, the presence at the interface of the oil phase suppresses the diffusion of odors into the aqueous phase, which is the continuous phase. .

(Other antioxidants)

The oil-in-water emulsion composition of the present disclosure may contain at least one type of antioxidant (hereinafter also referred to as "antioxidant B") different from the above-mentioned water-soluble tea extract (antioxidant A).

It does not specifically limit as antioxidant B.

Examples of the antioxidant B include at least one compound selected from the group consisting of ascorbic acid, ascorbic acid derivatives, and salts thereof, vitamin E and derivatives thereof such as tocopherol, vitamin A, retinoic acid, retinol, and acetic acid. Retinol, retinol palmitate, retinyl acetate, retinyl palmitate, tocopheryl retinoate, vitamin C and its derivatives, kinetin, sesamine, α-lipoic acid, coenzyme Q10, flavonoids, erythobic acid, propyl gallate, BHT (di-n-butylhydroxytoluene), BHA (butylhydroxyanisole), tretinoin, polyphenol, SOD (superoxide dismutase), phytic acid, astragalus extract, soybean extract, brier extract , a rosemary extract, etc. are mentioned.

Preferably as the antioxidant B, at least one compound selected from the group consisting of ascorbic acid, ascorbic acid derivatives, and salts thereof, vitamin E and derivatives thereof such as tocopherol, vitamin A, retinoic acid, retinol, Retinol acetate, more preferably at least one compound selected from the group consisting of ascorbic acid, ascorbic acid derivatives, and salts thereof, and vitamin E and derivatives thereof such as tocopherol, more preferably tocopherol and vitamin E and its derivatives.

The content of the antioxidant B in the oil-in-water emulsion composition of the present disclosure can be appropriately set according to the type of the antioxidant B in consideration of maintenance of emulsion stability of astaxanthin, etc.

The content of the antioxidant B is preferably 0.1 times or more and 100 times or less with respect to the mass of astaxanthin, more preferably 0.1 times or more and 10 times or less, and still more preferably 0.1 times or more and 5 times or less.

When at least one compound selected from the group consisting of ascorbic acid, ascorbic acid derivatives, and salts thereof is used as the antioxidant B in the oil-in-water emulsion composition of the present disclosure, ascorbic acid and its salts, Specifically, ascorbic acid, sodium ascorbate, potassium ascorbate, calcium ascorbate, etc. are mentioned.

Examples of ascorbic acid derivatives and salts thereof include ascorbic acid phosphate esters and salts, ascorbic acid sulfate esters and salts thereof, palmitic acid ascorbic acid esters and salts thereof, and the like.

A suitable aspect of the antioxidant B contained in the oil-in-water emulsion composition of the present disclosure is at least one compound selected from the group consisting of ascorbic acid, ascorbic acid derivatives, and salts thereof, and/or vitamin E such as tocopherol. and derivatives thereof.

In the oil-in-water emulsion composition of the present disclosure, as the antioxidant B, at least one compound selected from the group consisting of ascorbic acid, ascorbic acid derivatives, and salts thereof, and/or vitamin E and derivatives thereof such as tocopherol When contained, the total content is preferably 0.5 mass % to 6 mass % with respect to the total mass of the oil-in-water emulsion composition of the present disclosure, from the point of suppressing the precipitation of these compounds with time at low temperature. , 0.5 mass % - 4 mass % are more preferable, and 0.5 mass % - 3 mass % are still more preferable.

Ascorbic acid, ascorbic acid derivatives, and vitamin E, such as a compound of the group which consists of salts and/or tocopherol, and its derivative(s) may be one type, and may combine 2 or more types.

In the oil-in-water emulsion composition of the present disclosure, from the viewpoint of improving the stability of astaxanthin, the antioxidant B is preferably contained in the oily composition.

In the oil-in-water emulsion composition of the present disclosure, a water-soluble antioxidant can be used in addition to the water-soluble tea extract (antioxidant A) as long as the effect is not impaired. preferred in

From the viewpoint of emulsion stability, the content of the water-soluble tea extract and antioxidant B is preferably 0.6% by mass or more and 11% by mass or less, and more than 1.0% by mass or more and 8.0% by mass or less, based on the total mass of the oil-in-water emulsion composition. desirable.

[Enzyme-digested lecithin]

The oil-in-water emulsion composition of the present disclosure contains enzymatically decomposed lecithin.

Lecithin is a general name for a mixture containing various phospholipids such as phosphatidylcholine (PC). Enzyme-decomposed lecithin (also called lysolecithin) is a composition containing lysophosphatidylcholine in which one fatty acid in a phosphatidylcholine molecule is lost by an enzyme such as phosphatidase. In addition, in the oil-in-water emulsion composition of the present disclosure, the enzymatically decomposed lecithin contains so-called hydrogenated enzymatically decomposed lecithin, which is hydrogenated to improve oxidative stability by using the bound fatty acid as a saturated fatty acid.

From the viewpoint of obtaining a stable oil-in-water emulsion composition, the concentration of lysophosphatidylcholine contained in the enzymatically decomposed lecithin is preferably 18% by mass or more and 75% by mass or less, more preferably 18% by mass or more and 65% by mass or less, 18% by mass % or more and 30 mass % or less is more preferable.

The content rate of enzymatically decomposed lecithin in the oil-in-water emulsion composition of the present disclosure is preferably in the range of 0.00001 mass % or more and 7 mass % or less, with respect to the total mass of the oil-in-water emulsion composition, 0.00001 mass % or more and 5 mass % or less. It is more preferable that it is a range. From the viewpoint of ensuring emulsion stability while suppressing the taste derived from enzymatically decomposed lecithin, it is preferable that the content of enzymatically decomposed lecithin is 0.00001 mass % or more and 3.0 mass % or less with respect to the total amount of the oil-in-water emulsion composition.

As the enzymatically decomposed lecithin, commercially available enzymatically decomposed lecithin can be used. As the enzyme-decomposed lecithin, Benecot (registered trademark) BMI-40L (trade name, manufactured by Kao Corporation), Recimar (registered trademark) EL (trade name, manufactured by Riken Vitamin Corporation), SLP-Pasterizo (trade name, manufactured by Tsuji Seiyu Corporation), SLP -White Riso (trade name, manufactured by Tsuji Seiyu Co., Ltd.), SLP-White Riso H (trade name, manufactured by Tsuji Seiyu Co., Ltd.), and the like.

[Sucrose Fatty Acid Ester]

The oil-in-water emulsion composition of the present disclosure includes a sucrose fatty acid ester. When the oil-in-water emulsion composition of the present disclosure contains a sucrose fatty acid ester, the absorption of astaxanthin in the body is improved, and the emulsion stability with time is improved.

The oil-in-water emulsion composition of this indication may contain sucrose fatty acid ester individually by 1 type, and may contain it in combination of 2 or more type.

The HLB value of the sucrose fatty acid ester is 10 or more, preferably 12 or more, and more preferably 14 or more from the viewpoint of the absorption of astaxanthin in the body.

Further, the HLB value of the sucrose fatty acid ester is preferably 19 or less from the viewpoint of showing amphiphilicity.

It is preferable that carbon number of the fatty acid which comprises a sucrose fatty acid ester is 8 or more, for example from a viewpoint of showing amphiphilicity, and it is more preferable that it is 10-18.

Specific examples of the sucrose fatty acid ester include sucrose lauric acid ester, sucrose myristic acid ester, sucrose palmitic acid ester, sucrose stearic acid ester, and sucrose oleic acid ester.

Examples of commercially available sucrose fatty acid esters include Lyoto (registered trademark) Sugar Ester S1170 (trade name, sucrose stearic acid ester, HLB value: about 11 (catalog value), Mitsubishi Chemical Foods Co., Ltd.), Lyoto (registered trademark) Sugar Ester S1570 (trade name, sucrose stearate, HLB value: about 15 (catalog value), Mitsubishi Chemical Foods Co., Ltd.), Ryoto (registered trademark) sugar ester S1670 (brand name, sucrose stearate, HLB value: about 16 (catalog) Value), Mitsubishi Chemical Foods Co., Ltd.), Lyoto (registered trademark) Sugar Ester P1570 (trade name, sucrose palmitic acid ester, HLB value: Approx. 15 (catalog value), Mitsubishi Chemical Foods Co., Ltd.), Lyoto ( Registered trademark) Sugar Ester P1670 (trade name, sucrose palmitic acid ester, HLB value: about 16 (catalog value), Mitsubishi Chemical Foods Co., Ltd.), Ryoto (registered trademark) Sugar Ester L1695 (trade name, sucrose lauric acid ester) , HLB value: about 16 (catalog value), and the like.

In the oil-in-water emulsion composition of the present disclosure, from the viewpoint of improving the emulsion stability at low temperature, it is preferable that the ratio of mono-substituted products (also referred to as monoesters) to the total mass of sucrose fatty acid esters is 75% by mass or more. The proportion of the monoester may be 100% by mass (that is, all sucrose fatty acid esters are monoesters). The oil-in-water emulsion composition of the present disclosure more preferably contains a sucrose stearic acid ester as the sucrose fatty acid ester.

The content rate of the monoester body in sucrose stearic acid ester can be measured with the following method.

(Measurement of content rate of monoester form in sucrose stearic acid ester]

<Preparation of sample>

Sucrose stearic acid ester is added to tetrahydrofuran in an amount used to be 1 mg/mL and dissolved to obtain a sample solution.

<Measurement conditions>

Apparatus used: high performance liquid chromatography prominence (Shimazu Seisakusho Co., Ltd.)

Detection method: detection of corona charged particles

Column: Capcellpack C18 UG120 4.6 mm x 150 mm (Shiseido Co., Ltd.)

Column temperature: 40°C

Eluent: 0-20 min: water/methanol 50/50, 7 mM ammonium acetate; 20-30 min: methanol, 7 mM ammonium acetate

Flow: 1mL/min

Injection volume: 10 μL

<Method for calculating the content of monoesters>

The ratio of the peak area in the holding time of 17 minutes - 22 minutes with respect to the peak total area in the holding time of 15 minutes - 30 minutes in high performance liquid chromatography is computed, and let this be the content rate of a monoester body.

Moreover, the sucrose fatty acid ester containing 75 mass % - 100 mass % of a monoester form WHEREIN: Content of a monoester form can select and use 75 mass % or more in the commercial item shown below. In addition, a mixture of a plurality of the following commercial products may be mixed so that the content of the monoester body is 75% by mass or more. You may use the refined product raised to mass % or more.

As a commercial item, For example, Lyoto (trademark) Sugar Ester S-070, S-170, S-270, S-370, S-370F, S-570, S-770, S-970, S-1170, S -1170F, S-1570, S-1670 (above, trade name, manufactured by Mitsubishi Chemical Foods Co., Ltd.), DK Ester (registered trademark) SS, F160, F140, F110, F90, F70, F50, F-20W, F -10, FA-10E, Cosmetic Like, S-10, S-50, S-70, S-110, S-160, S-190 (above, brand name, manufactured by Daiichi Kogyo Seiyaku Co., Ltd.), etc. can be heard

Among them, Lyoto Sugar Ester S-1170, S-1170 F, S-1570, S-1670, DK Ester SS, F160, F140, F110, Cosmetic Like S-110, S-160, S-19, etc. are preferable. do. From the viewpoint of a high content of the monoester body, it is most preferably composed of only DK ester SS.

The content of sucrose fatty acid ester is, for example, from the viewpoint of astaxanthin absorption in the body, with respect to the total mass of the oil-in-water emulsion composition, preferably 0.1 mass% or more, more preferably 0.3 mass% or more, 0.5 It is more preferable that it is mass % or less.

Moreover, it is preferable that the content rate of a sucrose fatty acid ester is 10 mass % or less with respect to the total mass of an oil-in-water emulsion composition from a viewpoint of emulsion stability, for example.

[Polyglycerin fatty acid ester]

The oil-in-water emulsion composition of the present disclosure more preferably contains a polyglycerol fatty acid ester.

The polyglycerol fatty acid ester is preferably a polyglycerol fatty acid ester having at least one of a saturated fatty acid structure having 10 or more carbon atoms, a branched fatty acid structure, and an unsaturated fatty acid structure.

The polyglycerol fatty acid ester in the present disclosure preferably has an average degree of polymerization of 6 to 20, more preferably 8 to 16, still more preferably 8 to 10 polyglycerol, a saturated fatty acid having 10 to 22 carbon atoms, and carbon number and esters of fatty acids selected from unsaturated fatty acids having 8 to 22 carbon atoms and branched saturated fatty acids having 8 to 22 carbon atoms. Further, saturated fatty acids having 10 to 22 carbon atoms include linear saturated fatty acids having 10 to 22 carbon atoms and branched unsaturated fatty acids having 10 to 22 carbon atoms, and unsaturated fatty acids having 8 to 22 carbon atoms include linear unsaturated fatty acids having 8 to 22 carbon atoms. Fatty acids and branched unsaturated fatty acids having 8 to 22 carbon atoms are included.

When the polymerization degree of polyglycerol exists in the said range, emulsion stability becomes more favorable.

The polyglycerol fatty acid ester is preferably an ester having a fatty acid structure selected from a saturated fatty acid structure having 10 to 22 carbon atoms, an unsaturated fatty acid structure having 8 to 22 carbon atoms, and a branched saturated fatty acid structure having 8 to 22 carbon atoms, and having 10 to 22 carbon atoms. An ester of a fatty acid having at least one of a saturated fatty acid structure, a branched fatty acid structure having 16 to 22 carbon atoms, and an unsaturated fatty acid structure having 16 to 22 carbon atoms is more preferable, and a saturated fatty acid structure having 10 to 20 carbon atoms and a branched fatty acid having 16 to 18 carbon atoms An ester of a fatty acid having at least one of a structure and an unsaturated fatty acid structure having 16 to 18 carbon atoms is more preferable.

Examples of the fatty acid selected from saturated fatty acids having 10 to 22 carbon atoms include lauric acid, myristic acid, palmitic acid and stearic acid.

Examples of fatty acids selected from unsaturated fatty acids having 8 to 22 carbon atoms and branched saturated fatty acids having 8 to 22 carbon atoms include oleic acid, linoleic acid, and isostearic acid.

Although the polyglycerol fatty acid ester may be a monoester form or a diester form may be sufficient as it, it is preferable that it is a monoester form.

Moreover, polyglycerol fatty acid ester has surfactant ability. From a viewpoint of improving emulsion stability more, a compound of 5 or more is preferable, as for HLB, 8 or more compounds are more preferable, and 10 or more compounds are still more preferable. On the other hand, although the upper limit of HLB of polyglycerol fatty acid ester is not specifically limited, 20 or less are preferable and 18 or less are more preferable.

Preferred specific examples of the polyglycerol fatty acid ester include decaglycerin lauric acid ester, decaglycerin myristic acid ester, decaglycerin palmitic acid ester, decaglycerin stearic acid ester, hexaglycerin monooleic acid ester, decaglycerin monooleic acid ester, decaglycerin. Linoleic acid ester, decaglycerin isostearic acid ester, hexaglycerin isostearic acid ester, decaglycerin monoisostearic acid ester, etc. are mentioned, Among them, decaglycerol lauric acid ester, decaglycerin myristic acid ester, and decaglycerin pal. It is preferable that it is at least 1 sort(s) selected from the group which consists of mitic acid ester, decaglycerol stearic acid ester, isostearic acid polyglyceryl, and oleic acid polyglyceryl.

As the polyglycerol fatty acid ester, a commercial item may be used, and as a commercial item, for example, NIKKOL (registered trademark) Decaglyn 1-ISV, NIKKOL Decaglyn 1-OV, NIKKOL Decaglyn 1-LN, NIKKOL Decaglyn 1- manufactured by Nikko Chemicals Co., Ltd. PVEX, NIKKOL Decaglyn 1-SVEX, NIKKOL Decaglyn 1-L, NIKKOL Decaglyn 1-M, Mitsubishi Chemical Foods Co., Ltd. Lyotopoly Glyester O-15D, O-50D, Taiyo Chemical Co., Ltd. Sunsoft Q -17S, Riken Vitamin Co., Ltd. Poem J-0381V, etc. are mentioned.

Among these, more preferably, decaglycerin monolauric acid ester, decaglycerin stearic acid ester, decaglycerin monomyristic acid ester, decaglycerin palmitic acid ester, decaglycerin monooleic acid ester, decaglycerin monoisostearic acid ester and the like.

The oil-in-water emulsion composition of this indication may contain only 1 type, and may contain 2 or more types of polyglycerol fatty acid ester.

The content of the polyglycerol fatty acid ester in the oil-in-water emulsion composition of the present disclosure is preferably 2% by mass to 22% by mass, and more preferably 5% by mass to 22% by mass, based on the total mass of the oil-in-water emulsion composition. desirable.

The content of the polyglycerol fatty acid ester in the oil-in-water emulsion composition is within the above range, uniform and fine oily particles are easily obtained, and the emulsion stability of the oil-in-water emulsion composition at low temperature becomes more favorable.

From a viewpoint of improving the emulsion stability of the oil-in-water emulsion composition of this indication, it is preferable that content of polyglycerol fatty acid ester with respect to a sucrose fatty acid ester is 2 times amount - 13 times amount.

[water]

The oil-in-water emulsion of the present disclosure contains water.

There is no restriction|limiting in particular as water, Natural water, purified water, distilled water, ion exchange water, pure water, ultrapure water (Milli-Q water etc.), etc. can be used. In addition, Milli-Q water is ultrapure water obtained by the Milli-Q water manufacturing apparatus of Merck Millipore.

(emulsifier)

It is preferable that the oil-in-water emulsion composition of this indication contains an emulsifier.

The emulsifier in the oil-in-water emulsion composition of the present disclosure preferably contains at least one selected from the group consisting of enzymatically decomposed lecithin, sucrose fatty acid esters and polyglycerol fatty acid esters as described above, but oil-in-water emulsification of the present disclosure As long as the effect expected for the composition is not impaired, other emulsifiers may be contained.

As another emulsifier, you may contain lecithin, saponin, a sterol, etc. other than enzymatically-decomposed lecithin from a viewpoint that high pressure emulsification can be performed using only the emulsifier derived from a natural product, and time-lapse|temporal stability.

Here, "the emulsifier contains only at least one selected from the group consisting of enzymatically decomposed lecithin, sucrose fatty acid ester, and polyglycerol fatty acid ester" means, It shows that a content rate is 1 mass % or less with respect to the total mass of an oil-in-water emulsion composition. The content of the other emulsifier is preferably 0.001 mass% or more and 1 mass% or less, more preferably 0.01 mass% or more and 1 mass% or less, and still more preferably 0.01 mass% or more and 0.5 mass% or less.

When the emulsifier in the oil-in-water emulsion composition of the present disclosure contains only one or more selected from the group consisting of substantially enzymatically decomposed lecithin, sucrose fatty acid ester and polyglycerol fatty acid ester, enzymatically decomposed lecithin: sucrose fatty acid ester: poly It is preferable that glycerol fatty acid ester sets it as 0.00001:15:1-7:1:15 by mass ratio.

(polyhydric alcohol)

It is preferable that the oil-in-water emulsion composition of embodiment of this indication contains a polyhydric alcohol. From the viewpoint of emulsion stability, the polyhydric alcohol is preferably at least one selected from the group consisting of glycerin, sorbitol and reduced starch syrup, and more preferably at least one selected from glycerin or reduced starch syrup.

<Glycerin>

Glycerin is preferably used from the viewpoint of reducing the average particle diameter of the emulsified particles containing astaxanthin, and making it easy to keep the particle diameter small over a long period of time.

In the oil-in-water emulsion composition of the present disclosure, the content of glycerin is preferably in the range of 20% by mass or more and 60% by mass or less, and 20% by mass or more and 50% by mass or less with respect to the total mass of the oil-in-water emulsion composition. It is more preferable, and it is more preferable that it is the range of 30 mass % or more and 60 mass % or less.

In the oil-in-water emulsion composition of the present disclosure, the content of glycerin is preferably 1 to 100 times the mass of astaxanthin, more preferably 2 to 50 times or less, and 2 to 10 times or less more preferably.

<Reduced Starch Syrup>

Reduced starch syrup is one type of sugar alcohol, and is synthesized by reducing (that is, hydrogenating) starch syrup. Reduced starch syrup can adjust the viscosity by changing the composition of the raw material starch syrup (that is, saccharides), and is classified into high saccharide reduced starch syrup and low saccharide reduced starch syrup.

A low-saccharification reduced starch syrup is preferably used from the viewpoint of making the average particle diameter of the emulsified particles containing astaxanthin small and making it easy to keep the particle diameter small over a long period of time.

As the reduced starch syrup, from the viewpoint of maintaining the particle size of the emulsified particles containing astaxanthin and suppressing precipitation in the oil-in-water emulsion composition, a starch syrup obtained by reducing oligotose (straight-chain oligosaccharide) is particularly preferable. The origin of the oligotose is not particularly limited, but it is preferably a linear oligosaccharide containing maltotriose (G3) as a main component, a linear oligosaccharide containing maltotriose as a main component, and more preferably a low glucose and high molecular weight dextrin content. do.

The reduced starch syrup may be a commercial item.

Examples of commercial products of reduced starch syrup include oligotose (Sanwa Denpoon Kogyo Co., Ltd.), oligotose (trade name, Busan Food Science Co., Ltd.), ESWEE 30 (trade name, Busan Food Science Co., Ltd.), ESWEE 100 (brand name, Busan Food Science Co., Ltd.) Food Science Co., Ltd.) Oligotose H-70 (Mitsubishi Chemical Foods Co., Ltd.), Halodex (trade name, Hayashibara Corporation), Tetrup, Tetrup-H (trade name, Hayashibara Corporation), Pentrup (trade name, Hayashibara Corporation), Coupling Sugar (trade name, Hayashibara Corporation), Coupling Sugar-S (trade name, Hayashibara Corporation), etc. are mentioned.

The reduced starch syrup may be in either a solid (powdered form) or a liquid state containing a solvent (eg, water, etc.), but is preferably in a liquid form from the viewpoint of productivity.

Although the liquid reduced starch syrup of a commercial item contains 20 mass % or more and 40 mass % or less of water in many cases, as long as a desired effect is acquired, it may contain water outside this range.

The content rate of reduced starch syrup in the oil-in-water emulsion composition of the present disclosure is preferably in the range of 30% by mass or more and 70% by mass or less, and 35% by mass or more and 70% by mass or less with respect to the total amount of the oil-in-water emulsion composition. It is more preferable that it is a range, and it is more preferable that it is the range of 40 mass % or more and 70 mass % or less.

In addition, the "content rate of reduced starch syrup in the oil-in-water emulsion composition" described in the present disclosure means the content rate based on the mass of the solid content of the reduced starch syrup contained in the oil-in-water emulsion composition.

In the oil-in-water emulsion composition of the present disclosure, the content of reduced starch syrup is preferably 1 to 100 times the mass of astaxanthin, more preferably 2 to 50 times or less, and 2 to 10 times or less. it is more preferable

As polyhydric alcohol, it is preferable not to use polyhydric alcohol other than glycerol and reduced starch syrup, when using the oil-in-water emulsion composition of this embodiment for food.

[Other ingredients]

The oil-in-water emulsion composition of the present disclosure may contain, in addition to each component described above, one type or two or more types of optional other components as needed. Examples of other ingredients include physiologically active ingredients other than astaxanthin, such as nutritional ingredients, active ingredients, and pharmacological ingredients, pigments (not including astaxanthin), antioxidants, and liquid sugars such as starch syrup. . As such other components, it will not restrict|limit especially if it can be used for food-drinks and pharmaceuticals.

[Method for producing oil-in-water emulsion composition]

The oil-in-water emulsion composition of the present disclosure prepares a mixed solution obtained by mixing an oily composition containing astaxanthin and an oily component, and an aqueous composition containing water, sucrose fatty acid ester, and enzymatically decomposed lecithin, It is preferable to manufacture by a manufacturing method which includes emulsifying by a method. The water-soluble tea extract (antioxidant A) may be contained in either the oil phase composition or the aqueous phase composition, but from the viewpoint of effectively suppressing odor, the aqueous composition is preferably included.

Since the oil-in-water emulsion composition of the present disclosure contains the antioxidant A, while containing a high concentration of astaxanthin, the increase in the viscosity of the oil-in-water emulsion composition can be suppressed, and thus it is excellent in manufacturability.

The liquid mixture to be used for emulsification may be prepared by separately preparing the oil phase composition and the aqueous phase composition, then combining the prepared oil phase composition and the aqueous phase composition, or each component contained in the oil phase composition and the aqueous phase composition may be mixed together or You may prepare by mixing sequentially.

The oily composition may contain other optional components along with astaxanthin and oily components. As an antioxidant (antioxidant B) different from antioxidant A from the viewpoint of enhancing the stability of astaxanthin, at least one compound selected from the group consisting of ascorbic acid, ascorbic acid derivatives, and salts thereof, tocopherol, etc. It is preferable that at least one compound selected from the group consisting of vitamin E and derivatives thereof, and salts thereof is included.

In the aqueous composition, water, enzymatically digested lecithin, and sucrose fatty acid ester are included, and preferably, antioxidant A is included. The aqueous composition is preferably prepared by preparing a solution obtained by dissolving enzymatically decomposed lecithin and sucrose fatty acid ester in polyhydric alcohol, and then adding antioxidant A and water to this solution.

In one aspect of the method for producing an oil-in-water emulsion composition, for example, a) water, enzymatically decomposed lecithin, sucrose fatty acid ester, and antioxidant A are mixed and dissolved to obtain an aqueous composition, b) astaxanthin and , an oily component is mixed and dissolved to obtain an oily composition, and c) an aqueous phase composition and an oily composition are mixed under stirring to obtain a mixed solution, and the obtained mixed solution is emulsified and dispersed.

In the case of emulsification and dispersion, for example, after emulsification using a conventional emulsifying device using a shearing action such as a stirrer, impeller-type stirrer, homomixer, or continuous flow shearing device, through a high-pressure homogenizer, etc. It is preferable to use 2 or more types of emulsification apparatus together by a method. When a high-pressure homogenizer is used, the emulsified particles can be more uniformly aligned to a close particle diameter. For the purpose of further uniformizing the particle size, emulsification and dispersion may be performed a plurality of times.

The high-pressure homogenizer includes a chamber-type high-pressure homogenizer having a chamber in which a flow path of a processing liquid is fixed, and a homogenizing valve-type high-pressure homogenizer having a homogenization valve.

Since the homogenizer valve type high-pressure homogenizer can easily adjust the width of the flow path of the treatment liquid, the pressure and flow rate at the time of operation can be arbitrarily set, and the operation range is wide. It can be used preferably.

Although the degree of freedom of operation is low, since a mechanism for increasing the pressure is easy to make, a chamber-type high-pressure homogenizer can also be suitably used for applications requiring ultra-high pressure.

Examples of the chamber-type high-pressure homogenizer include a microfluidizer (manufactured by Microfluidics Co., Ltd.), a nanomizer (manufactured by Yoshida Kikai Kogyo Co., Ltd.), and an optimizer (manufactured by Sugino Machinery Co., Ltd.).

As the homogeneous valve-type high-pressure homogenizer, a Golin type homogenizer (manufactured by APV), a Lanier type homogenizer (manufactured by Lanier Corporation), a high-pressure homogenizer (manufactured by Niro Soabi Corporation), a homogenizer (manufactured by Sanwa Kikai) Co.), a high-pressure homogenizer (manufactured by Izumi Food Machinery Co., Ltd.), an ultra-high-pressure homogenizer (manufactured by Ika Corporation), and the like.

The pressure of the high-pressure homogenizer is preferably 50 MPa or more, more preferably 50 MPa or more and 250 MPa or less, still more preferably 100 MPa or more and 250 MPa or less.

It is preferable from the viewpoint of maintaining the particle size of the emulsified particles that the obtained oil-in-water emulsion composition is cooled through a cooler within 30 seconds, preferably within 3 seconds, immediately after passing through the chamber.

Moreover, when applying the obtained oil-in-water emulsion composition as an oil-in-water emulsion composition for soft capsule formulations, from a viewpoint of preventing the swelling of a soft capsule, etc., it is preferable to defoaming.

(Content of specific component in oil-in-water emulsion composition)

The odor derived from the raw material of astaxanthin originates from a plurality of substances, and as one of the causative substances, 2,4-heptadienal is mentioned. For this reason, from the viewpoint of reducing odor, the content (A) of 2,4-heptadienal is preferably 1000 ppb or less, more preferably 800 ppb or less, and further 500 ppb or less with respect to the total mass of the oil-in-water emulsion composition. desirable.

From the viewpoint of reducing swallowing when processed into beverages, especially algae odor (a fishy smell such as algae or fishy smell flowing from the nose after putting it in the mouth), a concentration of 1% by mass astaxan calculated according to the following formula (1) 200 ppb or less is preferable, as for content (B) of 2, 4- heptadiener converted per 100 mass parts of tin containing oil-in-water emulsions, 100 ppb or less is more preferable, and its 80 ppb or less is still more preferable.

(B) = (A)/astaxanthin content in oil-in-water emulsion composition... Formula (1)

The method for reducing the content of 2,4-heptadienal is not particularly limited,

(1) a method of preparing an oil-in-water emulsion composition of the present disclosure by using the purified astaxanthin after refining the raw material itself of astaxanthin by a known deodorizing method, and

(2) After preparing the oil-in-water emulsion composition of the present disclosure, any method such as purification by a known deodorizing method may be used.

As a known deodorizing method, a supercritical extraction method, a separation and purification method using a porous adsorbent such as activated carbon or silica gel, a masking method, an ozone oxidation method, a catalytic combustion method, etc. are mentioned.

The method for measuring the content of 2,4-heptadienal in the oil-in-water emulsion composition of the present disclosure is not particularly limited as long as the content of 2,4-heptadienal in the oil-in-water emulsion composition can be measured. Graph analysis is preferable, and gas chromatographic analysis or liquid chromatographic analysis is more preferable. From the viewpoint of accurately and relatively easily measuring 2,4-heptadienal contained in a trace amount in the oil-in-water emulsion composition, for example, headspace gas chromatograph mass spectrometry, purge & trap gas chromatograph mass spectrometry, etc. is mentioned as a more preferable method.

In the present disclosure, 2,4-heptadienal includes structural isomers.

(Viscosity of oil-in-water emulsion composition)

Although the viscosity of the oil-in-water emulsion composition of this indication is not specifically limited, From a viewpoint of manufacturing aptitude, such as emulsion dispersion, 600 mPa*s or less is preferable, and 500 mPa*s or less is more preferable. Although a lower limit in particular is not restrict|limited, For example, 50 mPa*s or more is mentioned.

The viscosity of the oil-in-water emulsion composition is not particularly limited as long as it is a method measured according to JIS Z8803 (2011), and a value measured at 25°C is used.

(Average particle diameter of oil-in-water emulsion composition)

The average particle diameter (volume average particle diameter) of the oil-in-water emulsion composition of the present disclosure is not particularly limited, but is preferably 5 nm to 200 nm, and from the viewpoint of good transparency and stability of the oil-in-water emulsion composition, 10 nm to 150 nm is more It is preferable, and it is more preferable to set it as 10 nm - 120 nm.

The average particle diameter (volume average particle diameter) of the oil-in-water emulsion composition can be measured with a commercially available particle size distribution meter or the like. As a method of particle size distribution measurement, optical microscopy, confocal laser microscopy, electron microscopy, atomic force microscopy, static light scattering method, laser diffraction method, dynamic light scattering method, centrifugal sedimentation method, electric pulse measurement method, chromatography method , ultrasonic attenuation method, etc., but are not limited thereto.

The particle size measurement is preferably performed by a dynamic light scattering method. As a commercially available measuring device using the dynamic light scattering method, Nanotrac UPA (trade name, Nikkiso Co., Ltd.), dynamic light scattering particle size distribution measuring device LB-550 (trade name, Horiba Seisakusho Co., Ltd.), a particle size analyzer with a concentration system Although FPAR-1000 (trade name, Otsuka Electric Co., Ltd.) etc. are mentioned, A measuring apparatus is not limited to these as long as a particle size measurement becomes possible based on the principle of a dynamic light scattering method.

The average particle diameter of the oil-in-water emulsion composition is, for example, Otsuka Denshi Co., Ltd., using a thick particle size analyzer FPAR1000 (trade name), 100-fold diluted oil-in-water emulsion composition into the sample cell in the apparatus, 25 ° C. The measured value is adopted, and it can be measured with a 50% cumulative particle diameter.

(Powder of oil-in-water emulsion composition)

The oil-in-water emulsion composition of the present disclosure can be made into a powdered form by drying.

The drying method at the time of powdering an oil-in-water emulsion composition is not specifically limited, Common methods, such as spray drying, kneading|mixing granulation, and freeze-drying, can be used.

The powdered oil-in-water emulsion composition can be used as an oil-in-water emulsion composition by suspending in a solvent containing water.

In the powdered oil-in-water emulsion composition, when improving the flowability in powder form and dispersibility at the time of suspension, it is preferable that the medium (ie, water) of the aqueous phase is removed as much as possible. What is necessary is just to perform removal of the medium of an aqueous phase by heat-drying means, such as spray drying by spray drying.

In the powdering of the oil-in-water emulsion composition, substances known in the art necessary for powdering, for example, excipients, binders, disintegrants, lubricants, sweeteners, preservatives, coating agents, stabilizers, fluidizing agents, thickening agents, solubilizing agents , and may further contain additives such as buffers, fragrances, colorants, and emulsifiers. In addition, content of the substance required for powdering is not specifically limited, It can set suitably.

When pulverizing the oil-in-water emulsion composition, if necessary, for example, polymer substances such as carboxymethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxypropyl methyl cellulose, polyvinyl alcohol and polyvinylpyrrolidone; natural high molecular substances such as gum arabic, gum tragacanth, and gelatin; Additives such as powdered lactose, casein, microcrystalline cellulose, starch, wheat flour, dextrin, and silicon dioxide may be added.

[purpose]

The oil-in-water emulsion composition of the present disclosure can be applied to various uses such as food or beverage (functional food or beverage, health food or beverage, etc.), pharmaceuticals, quasi-drugs, cosmetics, and the like, and among them, astaxanthin in high concentration Since it is possible to reduce the odor derived from the raw material of astaxanthin while containing it, the oil-in-water emulsion composition of the present disclosure is preferably used for food or beverage, and particularly preferably for beverage.

Formulations of foods, pharmaceuticals, quasi-drugs, or cosmetics to which the oil-in-water emulsion composition of the present disclosure is applied include, for example, liquid formulations and solid formulations (powders, granules, etc.) obtained by drying them.

The oil-in-water emulsion composition of the present disclosure can be applied to a liquid formulation and a solid formulation obtained by drying the same, for example, hard capsules, soft capsules, tablets, and the like. These formulations can also be prepared according to a conventional method by adding excipients, disintegrants, binders, lubricants, surfactants, water-soluble polymers, sweeteners, flavoring agents, acidulants, etc. according to the formulation. In addition, the solid preparation formed by applying the oil-in-water emulsion composition of the present disclosure may be coated or coated by a known method.

(soft capsule formulation)

One suitable application form of the oil-in-water emulsion composition of the present disclosure is a soft capsule formulation comprising the oil-in-water emulsion composition of the present disclosure. That is, the soft capsule formulation of the present disclosure is obtained by encapsulating the contents containing the oil-in-water emulsion composition of the present disclosure in a soft capsule shell.

The content in the soft capsule formulation of the present disclosure may contain 0.0001 mass % to 95 mass % of the oil-in-water emulsion composition of the present disclosure with respect to the total amount of the soft capsule formulation.

As the base material for forming the soft capsule film (hereinafter also simply referred to as a film) (hereinafter also referred to as a film-forming composition), a substrate containing agar, gelatin or gellan gum as a main component is preferable from the viewpoint of transparency of the film and the like. For example, a soft capsule shell is prepared from a gelatin coating liquid containing gelatin, a plasticizer, and water when gelatin is used as the base material for the coating film. Examples of the gelatin used include gelatin, acid gelatin, alkaline gelatin, peptide gelatin, low molecular weight gelatin, and gelatin derivatives.

Moreover, the soft capsule membrane|film|coat may contain the plasticizer, As a plasticizer, Glycerin; glycols such as propylene glycol and polyethylene glycol; sugars such as corn syrup, sucrose, fructose, sorbitol, and mannitol; and water-insoluble celluloses such as crystalline cellulose, starch, low-substituted hydroxypropyl cellulose and ethyl cellulose. In addition, these plasticizers may be used individually by 1 type, and may be used in combination of 2 or more type arbitrarily. As a plasticizer, glycerol is preferable.

In the soft capsule preparation (content or film), an additive component normally used in the field of food may be appropriately blended as long as the effect expected in the soft capsule preparation of the present disclosure is not impaired. Examples of the additive component include colorants such as pigments or pigments, preservatives, fragrances, fruit juices, various vitamins, animal and plant extracts, amino acids, minerals, colorants, thickeners, pH adjusters, preservatives, disintegrants, surfactants, flavoring agents, odorants , sweeteners, organic acids, and the like, and may be appropriately selected from these components as needed and blended.

As a manufacturing method of a soft capsule formulation, for example, (i) preparing the contents of a capsule and a composition for film formation, respectively (hereinafter referred to as a composition preparation step), (ii) encapsulating the contents in the obtained composition for film formation and molding and drying to obtain an intermediate formulation (hereinafter referred to as a molding step), and (iii) drying the obtained intermediate formulation (hereinafter referred to as a drying step) to obtain a suitable manufacturing method.

In a composition preparation process, the content containing the oil-in-water emulsion composition of this indication, and the composition for film formation are prepared, respectively. The method for preparing the oil-in-water emulsion composition of the present disclosure is as described above. Moreover, what is necessary is just to prepare the composition for film formation as a solution in which the predetermined|prescribed component contained in the composition was dissolved.

In a shaping|molding process, the content containing the oil-in-water emulsion composition of this indication is enclosed in the coating film formed using the composition for film formation, and it shape|molds and dries, and obtains an intermediate formulation.

In the soft capsule formulation of the present disclosure, the encapsulation of the contents into the composition for film formation can be performed according to a conventionally known method for producing a soft capsule formulation, for example, a flat plate method or a rotary die method.

In a drying process, the intermediate preparation obtained in a shaping|molding process is dried. By passing through this process, the soft capsule formulation which is a finished product is obtained.

The drying means applied to a drying process is not specifically limited, Well-known drying machines, such as a tumbler dryer (namely, rotary drum type dryer), can be used.

Conditions in particular at the time of drying, such as temperature and time, are not restrict|limited, According to the kind of component contained in a food composition, and a component contained in a capsule film, it can adjust suitably.

There is no restriction|limiting in particular as a shape of a soft capsule formulation, Any shape, such as an oval (OVAL), a rectangle (OBLONG), and a sphere (ROUND), can be taken. Since it is set as these shapes, a method or apparatus well-known in the art can be applied.

(beverage)

The oil-in-water emulsion composition of the present disclosure can be diluted with a solvent such as water to obtain a beverage.

It is good also as a beverage by adding a solvent, such as water, to the oil-in-water emulsion composition of this indication used as solid preparations, such as a powder.

In the case of a beverage, the oil-in-water emulsion composition of the present disclosure may be contained in preferably 0.0001 mass% to 50 mass%, more preferably 0.1 mass% to 20 mass%, with respect to the total amount of the drink. can

By mixing a sweetener, a flavoring agent, an acidulant, a thickening stabilizer, an antioxidant, etc. with a beverage, the taste etc. as a beverage can be adjusted suitably.

As a sweetener, any material may be sufficient as long as it is a material which shows sweetness. For example, fruit juice, saccharide|sugar, a high-sweetness sweetener, etc. are mentioned. Examples of saccharides include monosaccharides such as glucose, fructose, galactose, and fructose, disaccharides such as sucrose, lactose, and palatinose, fructooligosaccharides, isomaltooligosaccharides, galactooligosaccharides, oligosaccharides such as palatinose, For example, monosaccharide alcohols such as erythritol, sorbitol, xylitol, and mannitol; disaccharide alcohols such as maltitol, isomaltitol, and lactitol; Sugar alcohols, such as tetrasaccharide or more alcohols, such as these, are mentioned. For example, stevia, aspartame, saccharin, glycyrrhizin, thaumatin, sucralose, acesulfame K etc. are mentioned as a high-sweetness sweetener.

As a fragrance|flavor, a natural fragrance|flavor, a synthetic fragrance|flavor, etc. are mentioned, for example.

As a natural fragrance|flavor, the fragrance component containing material etc. which were prepared by a conventional method using herb root, bark, flowers, fruit, a fruit skin, or other animals and plants as raw materials are mentioned, for example. Moreover, the essential oil etc. which processed and separated a natural raw material by the steam distillation method, a compression method, an extraction method, etc. are mentioned as a natural fragrance|flavor.

Examples of the synthetic fragrance include coffee-derived fragrance, black tea-derived fragrance, green tea-derived fragrance, oolong tea-derived fragrance, cocoa-derived fragrance, herb-derived fragrance, spice-derived fragrance, fruit-derived fragrance, and the like.

The beverage may be a canned beverage by filling the container.

As a container used for a drink, what is necessary is just what is used as a container for drinks, For example, a PET bottle, a paper carton, a glass container, an aluminum can, a steel can, etc. are mentioned.

The range of 1.0-9.0 is preferable, as for pH in 20 degreeC, as for pH of a beverage, the range of 1.5-7.0 is more preferable, The range of 2.0-5.0 is especially preferable. If it is pH 1.0 or more, it is suitable for drinking, and if it is pH 9.0 or less, the flavor stabilization effect as a beverage can fully be acquired.

Example

Hereinafter, the oil-in-water emulsion composition of the present disclosure will be described in detail in Examples. However, the oil-in-water emulsion composition of the present disclosure is not limited to their Examples. In addition, unless otherwise specified, "%" and "part" are based on mass.

The oil-in-water emulsion composition prepared in the Examples can be suitably used for soft capsule formulations, foods (functional foods, health foods, beverages, etc.), pharmaceuticals, quasi-drugs, or cosmetics.

[Examples 1A to 11A, Comparative Examples 1A to 4A]

1A. Preparation of oil-in-water emulsion composition

Each component was mixed and dissolved in the composition shown in Table 1 and Table 2, respectively, and an aqueous phase composition and an oil phase composition were obtained, respectively. The obtained aqueous phase composition and oil phase composition were mixed while stirring using a magnetic stirrer to obtain a liquid mixture.

In addition, while continuing stirring with a stirrer, the mixture is heated to 70° C. for 30 minutes, and then shearing force is applied for 3 minutes at 3000 rpm (revolutions per minute, hereinafter the same) using a TK homo mixer (Primix Co., Ltd.), and oil-in-water An emulsion composition was prepared.

The oil-in-water emulsion composition of Examples 1A to 11A and Comparative Examples 1B to 4B was obtained by treating the obtained oil-in-water emulsion composition three times at a pressure of 200 MPa with a high-pressure dispersing device, Starburst Mini Machine (Sugino Machine Co., Ltd.) got it

The detail of each component shown in Table 1 and Table 2 is as follows.

Astaxanthin: ASTOTS (registered trademark)-S (trade name, hematococcus algae-derived astaxanthin-containing oil, astaxanthin 20% by mass, medium-chain fatty acid triglyceride content, Fujifilm Co., Ltd.)

・Tri (caprylic/capric acid) glyceryl: O.D.O (trade name, Nisshin Oilio Co., Ltd.)

· Enzyme-digested lecithin: SLP-White Rizo (trade name, Tsuji Seiyu Co., Ltd.)

・Sucrose fatty acid ester 1: DK ester SS (trade name, Daiichi Kogyo Seiyaku Co., Ltd.)

・Sucrose fatty acid ester 2: Lyoto sugar ester S-1670 (trade name, Mitsubishi Chemical Foods Co., Ltd.)

・Polyglycerin fatty acid ester: Poem J-0381 V (trade name, Riken Vitamin Co., Ltd.)

Mixed tocopherols (vitamin E mixture): Riken E Oil 800 (trade name, Riken Vitamin Co., Ltd.)

Glycerin: food additive grade

Water-soluble tea extract 1: Polyphenone 70S (trade name, containing 80% tea polyphenol, Mitsui Norin Co., Ltd.)

Water-soluble tea extract 2: Sunfood 100 (trade name, Mitsubishi Chemical Foods Co., Ltd.)

Water-soluble antioxidant: sodium L-ascorbate (Mitsubishi Chemical Foods Co., Ltd.)

Oil-soluble tea extract: Sunfood oily (trade name, Mitsubishi Chemical Foods Co., Ltd.)

・Antioxidant B: Rosemary extract (Nissin Oilio Co., Ltd.)

Water: Purified water

The numerical value shown about each component in Table 1 and Table 2 shows the content rate (mass %) of each component.

In Table 1 and Table 2, description of "-" in content of each component shows that the said component is not contained.

2A. Evaluation of oil-in-water emulsion composition A1

Each obtained oil-in-water emulsion composition was evaluated by the following method. A result is shown in Table 1 and Table 2.

2A-1. Odor of oil-in-water emulsion composition

The obtained oil-in-water emulsion composition was melt|dissolved in mineral water so that the density|concentration of astaxanthin might be 6 mg/100g, and the beverage composition was prepared.

The prepared beverage composition was drank by 8 subjects, and the algae odor (a fishy smell like algae or fishy smell that flows out from the nose after being put in the mouth) was evaluated by the numerical values in the evaluation steps shown below. The average value which carried out the arithmetic mean of each obtained numerical value was given a rank by A, B, or C shown below, and it was set as the evaluation result.

<Evaluation stage>

-2: No algae smell, easy to drink.

0: There is a slight algae smell, but it is drinkable.

+2: There is a distinct algae smell, and it is not drinkable.

<average value>

A: -1.0 points or less

B: More than -1.0 points and less than 0.5 points

C: More than 0.5 points and less than 1.5 points

D: over 1.5 points

2A-2. Emulsion stability at room temperature

After the oil-in-water emulsion composition was prepared and stored at 60°C for 1 week, the presence or absence of precipitation in the oil-in-water emulsion composition was visually observed and evaluated according to the evaluation criteria shown below.

Here, the storage period of one week at 60°C corresponds to the period of storage at room temperature (25°C) for about one year. Also, A and B are practically no problem levels.

<Evaluation criteria>

A: No precipitation was observed in the oil-in-water emulsion composition.

B: Some floating matter is confirmed in the oil-in-water emulsion composition.

C: Precipitation and precipitation are confirmed in the oil-in-water emulsion composition, or phase separation is confirmed.

[Table 1]

[Table 2]

The oil-in-water emulsion compositions of Examples 1A to 11A were all oil-in-water emulsion compositions in which the odor derived from the raw material of astaxanthin was suppressed, and the emulsion stability at room temperature was also excellent.

3A. Evaluation of oil-in-water emulsion composition A2

The following method evaluated the oil-in-water emulsion composition of Examples 1A-11A. A result is shown in Table 3.

3A-1. Viscosity of oil-in-water emulsion composition

After preparing the oil-in-water emulsion composition, the viscosity (mPa·s) at 25°C was measured using a vibrating viscometer (product number: VM-10A-L, Asone Co., Ltd.).

3A-2. Manufacturing aptitude of oil-in-water emulsion composition

For each oil-in-water emulsion composition, it was confirmed what kind of pass treatment was possible with a high-pressure emulsifying device (Starburst Mini, manufactured by Sugino Machinery Co., Ltd.) by a nozzle spraying method using a spray nozzle having a pressure of 200 MPa and a hole diameter of 0.15 µm. The evaluation criteria shown below evaluated.

<Evaluation criteria>

A: 3 passes or more available

B: 2 passes available

C: 1 pass is possible, but it is slightly difficult to process more than 2 passes

3A-3. Emulsion stability at low temperature

After the oil-in-water emulsion composition was prepared and stored at 10°C for one month, the presence or absence of precipitation in the oil-in-water emulsion composition was visually observed and evaluated according to the standards shown below. Also, A and B are practically no problem levels.

<Evaluation criteria>

A: No precipitation was observed in the oil-in-water emulsion composition.

B: Some floating matter is confirmed in the oil-in-water emulsion composition.

[Table 3]

The oil-in-water emulsion compositions of Examples 1A to 11A were all oil-in-water emulsion compositions excellent in emulsion stability at low temperatures. Moreover, all of Examples 1A-11A were capable of high-pressure emulsification treatment and were excellent in manufacturing aptitude. In particular, it was found that Examples 3A to 5A and 7A to 11A having a viscosity of 600 mPa·s or less were more preferable for producing an oil-in-water emulsion having a small particle diameter in which three or more passes could be processed.

[Examples 1B to 12B, Comparative Example 1B]

1B. Preparation of oil-in-water emulsion composition

Each component was mixed and dissolved in the composition shown in Table 4, respectively, and an aqueous phase composition and an oil phase composition were obtained, respectively.

The oily composition was treated with activated carbon after preparation. The presence or absence of activated carbon treatment was described in Table 4.

The obtained aqueous phase composition and oil phase composition were mixed while stirring using a magnetic stirrer to obtain a liquid mixture.

Further, the mixture was heated to 70° C. for 30 minutes while stirring with a stirrer, and then a shear force was applied at 3000 rpm for 3 minutes using a TK homomixer (Primix Co., Ltd.) to prepare an oil-in-water emulsion composition.

The oil-in-water emulsion composition of Examples 1B to 12B and Comparative Example 1B was obtained by treating the obtained oil-in-water emulsion composition three times at a pressure of 200 MPa with a high-pressure dispersing device, Starburst Mini Machine (Sugino Machine Co., Ltd.).

The detail of each component shown in Table 4 is as follows.

Hematococcus algae pigment 1: ASTOTS (registered trademark)-S (trade name, hematococcus algae-derived astaxanthin-containing oil, color value 2000-5000, astaxanthin 20% by mass, medium-chain fatty acid triglyceride content , content of 2,4-heptadienal A: 6.2 mg/kg, concentration of 1 mass % astaxanthin-containing oil-in-water emulsion content of 2,4-heptadienal converted per 100 parts by mass (B): 31.0 mg /kg, Fujifilm Co.).

Hematococcus algae pigment 2: ASTOTS (registered trademark)-SS (trade name, hematococcus algae-derived astaxanthin-containing oil, color value 5000-7500, astaxanthin 35% by mass, medium-chain fatty acid triglyceride content , 2,4-heptadienal content A: 4.5 mg/kg, 1 mass % astaxanthin-containing oil-in-water emulsion content of 2,4-heptadienal converted per 100 parts by mass (B): 12.9 mg/kg, Fujifilm Co.)

・Tri (caprylic/capric acid) glyceryl: O.D.O (trade name, Nisshin Oilio Co., Ltd.)

· Enzyme-digested lecithin: SLP-White Rizo (trade name, Tsuji Seiyu Co., Ltd.)

・Sucrose fatty acid ester 1: DK ester SS (trade name, Daiichi Kogyo Seiyaku Co., Ltd.)

・Polyglycerol fatty acid ester 1: Nikkol Decaglyn1-M (trade name, Nikko Chemicals Co., Ltd., Decaglycerin Monomyristic Acid Ester)

・Polyglycerol fatty acid ester 2: Nikkol Decaglyn1-L (trade name, Nikko Chemicals Co., Ltd., decaglycerin monolauric acid ester)

Mixed tocopherols (vitamin E mixture): Riken E Oil 800 (trade name, Riken Vitamin Co., Ltd.)

Glycerin: food additive grade

Water-soluble tea extract 1: Polyphenone 70S (trade name, tea polyphenol content: 80%, medium epigallocatechin gallate content: 20-30%, Mitsui Norin Co., Ltd.)

Water-soluble tea extract 2: N-theafuran 90S (trade name, tea polyphenol content: 90%, medium epigallocatechin gallate content: 40-50%, Itoen Co., Ltd.)

Water-soluble tea extract 3: Sanphenone EGCG-OP (trade name, tea polyphenol content: 95%, medium epigallocatechin gallate content: 94% or more, Taiyo Chemical Co., Ltd.)

Water: Purified water

The numerical value shown about each component in Table 4 shows the content rate (mass %) of each component.

In Table 4, the description of "-" in the content of each component indicates that the corresponding component is not contained.

2B. Evaluation of oil-in-water emulsion composition B1

Each obtained oil-in-water emulsion composition was evaluated by the following method. A result is shown in Table 4.

2B-1. Odor of oil-in-water emulsion composition

Evaluation was performed in the same manner as in 2A-1 described above.

2B-2. Emulsion stability at room temperature

Evaluation was performed in the same manner as in 2A-2 described above.

3B. Evaluation of oil-in-water emulsion composition B2

The following method evaluated the oil-in-water emulsion composition of Examples 1B-12B and Comparative Example 1B. A result is shown in Table 4.

3B-1. Viscosity of oil-in-water emulsion composition

Evaluation was performed in the same manner as in 3A-1 described above.

3B-2. Preparation of oil-in-water emulsion composition

Evaluation was carried out in the same manner as in 3A-2 described above.

3B-3. Emulsion stability at low temperature

Evaluation was performed in the same manner as in 3A-3 described above.

[Table 4]

4B. Evaluation of oil-in-water emulsion composition B3

Content of 4B-1.2,4-heptadienal

For the oil-in-water emulsion compositions of Examples 2B, 7B and Comparative Example 1B, the content of 2,4-heptadienal was measured by the following method. A result is shown in Table 5.

(1) Preparation of calibration curve

2,4-heptadienal standard solution (concentration of 1000 ppm, Fujifilm Wako Junyaku Co., Ltd.) was prepared so that the concentration of 2,4-heptadienal was 10 ppb, 50 ppb, 250 ppb, 500 ppb, and 40 mass % of glycerin It diluted using the aqueous solution (Fujifilm Wako Junyaku Co., Ltd.), and the sample solution for calibration curve preparation was prepared, respectively.

Then, 1 mL of each sample solution for preparing a calibration curve is collected and sealed in a head space vial to prepare a sample for calibration curve production, and a head space gas chromatograph mass spectrometer (HS/GC-MS: Head Space-Gas Chromatograph/Mass Spectrometer) ) to measure the peak area. A measurement apparatus and measurement conditions are shown below.

Then, the peak area corresponding to the concentration of 2,4-heptadienal in each sample for preparing a calibration curve was plotted to prepare a calibration curve.

<Measuring device>

HS Department: HS-20, Shimadzu Seisakusho

GC-MS Department: GSMS-QP2020, Shimadzu Seisakusho

<Measurement conditions>

・Headspace conditions

Oven temperature: 80℃

Keep warm time: 30min

·GC/MS conditions

Column: 40℃(5min hold)→250℃(15min hold)

Split ratio: 10:1

Detection: SIM mode (m/z=81)

(2) Evaluation of the content of 2,4-heptadienal in the sample

1 mL of each of the oil-in-water emulsion compositions of Examples 2B, 7B and Comparative Example 1B were collected and sealed in a headspace vial to prepare each measurement sample, and according to the measurement device and measurement conditions described in (1), the headspace The peak area was measured with a gas chromatograph mass spectrometer.

The obtained peak area was interpolated to the analytical curve prepared in (1), and the content (A) of 2, 4- heptadienal in each measurement sample was calculated|required.

Then, according to Formula (1), content (B) of 2, 4- heptadienal converted per 100 mass parts of concentration 1 mass % astaxanthin containing oil-in-water emulsion was calculated|required.

(B) = (A)/astaxanthin content in oil-in-water emulsion composition... Formula (1)

4-2. Assessment of odors by expert panelists

The odor of the oil-in-water emulsion composition of Example 2B, Example 7B, and Comparative Example 1B was evaluated by 12 expert panelists who satisfy|fill the criteria for panel selection shown below. Specifically, each oil-in-water emulsion composition of Example 2B, Example 7B and Comparative Example 1B was left at room temperature (25° C.), the sample temperature was returned to room temperature, and then consumed by a professional panelist, in the following steps It evaluated and the average value which carried out the arithmetic mean was used.

A result is shown in Table 5.

<Criteria for panel selection>

The taste of an aqueous solution of 0.4% sucrose, 0.02% citric acid, 0.13% salt, 0.05% sodium glutamate and 0.03% caffeine was judged to be a normal person by the standard for panel selection [Daichi Yakuhin Sangyo Co., Ltd.] who can.

<Evaluation criteria>

The intensity of the degree of feeling the algae smell (a fishy smell like algae or fishy smell flowing from the nose after putting it in the mouth) was assigned a rank in the same manner as below, with Comparative Example 1B being 0 points.

3 points: very strong

2 points: strong

1 point: Slightly strong

0 points: no car

-1 point: slightly weak

-2 points: weak

-3 points: very weak

With respect to the obtained scoring results, the evaluation of the significant difference by the t-test was confirmed, and a significance level of 1% was confirmed in all cases.

[Table 5]

From the above results, 2,4-heptadienal content (A) was reduced, and in Examples 2B and 7B containing a water-soluble tea extract containing 20 mass % or more of tea polyphenol, 2,4- The evaluation of odor by the expert panelists was better than Comparative Example 1B in which the heptadienal content A was not reduced. In particular, Example 2B in which the content (B) of 2,4-heptadienal converted per 100 parts by mass of the oil-in-water emulsion containing 1% by mass of astaxanthin was reduced was a more favorable evaluation result.

Further, from the above results, it was shown that there is a correlation between the content of 2,4-heptadienal and the degree of odor perceived by humans.

As for the indication of the JP Patent application 2017-190995 for which it applied on September 29, 2017, the whole is taken in into this specification by reference.

All documents, patent applications, and technical standards described in this specification are incorporated herein by reference to the same extent as if each individual document, patent application, and technical standard was specifically and individually indicated to be incorporated by reference. .

Claims (14)

astaxanthin, oily ingredients, enzymatically digested lecithin, sucrose fatty acid ester, polyglycerin fatty acid ester, water-soluble tea extract, and water;
The sucrose fatty acid ester has 10 to 18 carbon atoms in the fatty acid constituting the sucrose fatty acid ester,
The polyglycerol fatty acid ester is an ester of a fatty acid selected from polyglycerin having an average degree of polymerization of 6 to 20, saturated fatty acids having 10 to 22 carbon atoms, unsaturated fatty acids having 8 to 22 carbon atoms, and branched saturated fatty acids having 8 to 22 carbon atoms,
The content of astaxanthin with respect to the total mass of the oil-in-water emulsion composition is 0.1% by mass to 20.0% by mass,
The water-soluble tea extract contains 80% by mass or more of tea polyphenols based on the total mass of the water-soluble tea extract,
The content of the water-soluble tea extract relative to the total mass of the oil-in-water emulsion composition is 0.3% by mass or more and 5.0% by mass or less,
The oil-in-water emulsion composition, wherein the tea polyphenol contains at least one or more of epigallocatechin gallate or epigallocatechin in an amount of 10% by mass or more. The method according to claim 1,
The oil-in-water emulsion composition in which the content rate of polyglycerol fatty acid ester with respect to the total mass of the oil-in-water emulsion composition is 2 mass % or more and 22 mass % or less. The method according to claim 1 or 2,
The oil-in-water emulsion composition, wherein the content of the polyglycerol fatty acid ester with respect to the sucrose fatty acid ester is 2 to 13 times. The method according to claim 1 or 2,
An oil-in-water emulsion composition comprising enzyme-decomposed lecithin in an amount of 0.00001 mass % or more and 7 mass % or less with respect to the total mass of the oil-in-water emulsion composition. The method according to claim 1 or 2,
The oil-in-water emulsion composition, wherein the content rate of astaxanthin with respect to the total mass of the oil-in-water emulsion composition is 1% by mass to 20.0% by mass. The method according to claim 1 or 2,
The oil-in-water emulsion composition, wherein the sucrose fatty acid ester contains at least one selected from the group consisting of sucrose lauric acid ester, sucrose myristic acid ester, sucrose palmitic acid ester, sucrose stearic acid ester, and sucrose oleic acid ester. The method according to claim 1 or 2,
Polyglycerol fatty acid ester, decaglycerin lauric acid ester, decaglycerin myristic acid ester, decaglycerin palmitic acid ester, decaglycerin stearic acid ester, hexaglycerin monooleic acid ester, decaglycerin monooleic acid ester, decaglycerin linoleic acid ester, An oil-in-water emulsion composition comprising at least one selected from the group consisting of decaglycerin isostearic acid ester, hexaglycerin isostearic acid ester, and decaglycerin monoisostearic acid ester. The method according to claim 1 or 2,
The oil-based component is an oil-in-water emulsion composition comprising at least one selected from the group consisting of medium-chain fatty acid triglycerides having 8 to 10 carbon atoms of fatty acids constituting the triglyceride, and animal and vegetable oils and fats. delete delete delete delete delete delete