KR102442059B1 - Manufacturing method of plant virus disease prevention and spread prevention agent based on micronutrients - Google Patents

Abstract

The present invention relates to a method for preparing a composition for suppressing and preventing the spread of plant viruses comprising micronutrients, and to a method for spraying a formulation made of the composition onto stems and leaves of a virus-infected plant. More specifically, a pest-controlling composition according to the present invention comprises nitrogen, phosphoric acid, potassium oxide, zinc, boron, copper and a surfactant, and shows an effect on viral diseases of Cucurbitaceae crops or Solanaceae crops.

Description

Method for producing a composition for preventing the onset and spreading of plant virus containing microelements {Manufacturing method of plant virus disease prevention and spread prevention agent based on micronutrients}

The present invention relates to a method for producing a composition for preventing the onset of and spreading of a plant virus comprising a trace element that can be applied by foliage to a virus-infected plant, and more specifically, the composition for controlling a plant virus disease includes nitrogen, phosphoric acid, potassium, It is a composition comprising zinc, boron, copper and a surfactant, and is characterized in that it exhibits an effect on plant virus diseases of gourd crops or solanaceae crops.

Viruses are composed of nucleic acids and proteins, and are organisms that infect and multiply host cells. Depending on the host, there are animal viruses, plant viruses, bacterio phages, and mycoviruses. Plant viruses are viruses that infect plant cells. They do not have a self-replicating organ, so they proliferate within cells after invading host cells, and spread in various ways such as insects, seeds, soil, mechanical contact, vegetative bodies, fungi, and nematodes. About 2,000 plant viruses have been isolated and reported worldwide, and about 120 plant viruses have been reported in Korea.

Plant virus, called plant ADIS, is a nuclear protein that proliferates only in living plant cells and causes disease. Based on its strong infectivity and proliferative power, it causes a great economic loss in agricultural productivity. It is causing serious damage to the overall agricultural production, such as lowering production, lowering quality, and deterioration of quality. In Korea, especially, the damage to soybean (pepper, tomato, etc.) and gourd (cucumber, watermelon, melon, etc.) crops, which are facility vegetable crops with high economic value added, is large.

In general, in the case of plant viruses, unlike fungi or bacteria, it is difficult to selectively control only viruses because they share a metabolic process within the cells of the host plant. Once a plant is infected with a virus, it is practically incurable, and there are currently no drugs that can control it. Therefore, unlike the case of other pathogenic microorganisms, it is more realistic to use a preventive control method for plant viruses. Basically, it is desirable to use an appropriate combination of cultivation of resistant varieties, removal of infectious agents, blocking of transmission pathways, biological control, and use of virus inhibitors.

Many studies have been conducted on antiviral substances that are effective in controlling viruses by directly acting on them. These are mainly metabolites, microbial-derived substances, extracts of higher plants, other biological materials, chemical synthetic products, etc., and their antiviral action against various substances has been investigated. These antiviral agents are sometimes classified into infection inhibitors, proliferation inhibitory substances, local spot inducers, virus movement inhibitors, and the like according to their mechanism of action.

Most of the antiviral activity of substances extracted from higher plants shows an anti-infection effect. So far, it is known that the juices of many plants have antiviral effects, and among them, extracts such as jagongong, marigold, amaranth, and peony flower have been reported to have an effective inhibitory effect. As a technology related thereto, a composition for controlling a plant virus containing extracts of mycelium, Shiza, oyster mushroom or spectators (Registration Patent Publication No. 823085), and a plant virus controlling composition comprising a blackcurrant extract (Registration Patent Publication No. 1457869) ), etc. However, despite many such studies, very few have been developed as practical antiviral agents. The reason is that the effect in the laboratory is poor in the actual field, or it is difficult to apply to plants in practical use.

As such, since a product for controlling plant viruses has not been developed so far, the damage caused by the virus is serious. This is because of the characteristics of plant viruses that use host cell replication machinery and organelles for replication. In order to control plant viruses, the control substance must act directly on the virus or act on the replication mechanism and organelle of the host cell. it is known

Accordingly, the present inventors selected and put efforts to put into practical use a substance having antiviral effect that penetrates into crop cells to inhibit plant virus replication and block virus movement in plants, and as a result, the control composition of the present invention was selected and the A manufacturing method was established. Furthermore, the present invention was completed by confirming the effect of controlling the virus disease by the method of spraying the control composition on the leaves of the Solanaceae and Cucurbitaceae crops.

Republic of Korea Patent Publication No. 823085 (Composition for controlling plant virus) Republic of Korea Patent Publication No. 1457869 (plant virus control composition comprising blackcurrant extract) Republic of Korea Patent Publication No. 2019-32209 (Composition for controlling plant virus and use thereof)

An object of the present invention is to be able to use a method of spraying on the foliage of a plant, by temporarily stopping the activity of a virus infected with cells in a plant (virostatic effect) and inhibiting the movement between cells, thereby transferring the virus to a healthy wine. And to provide a composition for controlling plant viruses that can overcome the effects of viruses by preventing infection and promoting plant growth.

The technical problems to be solved by the invention are not limited to the technical problems mentioned above, and other technical problems not mentioned will be clearly understood by those of ordinary skill in the art to which the present invention belongs from the description below. will be able

The composition for controlling plant viruses for foliar spraying of the present invention is nitrogen (N) 0.1 to 2.5%, phosphoric anhydride (P ₂ O ₅ ) 0.2 to 3.0%, potassium oxide (K ₂ O) 0.1 to 1.0%, boron oxide (B ₂ O ₃ ) 0.05 to 1.5%, zinc (Zn) 0.05 to 3.0%, copper (Cu) 0.05 to 4.0%, and 1.0 to 10.0% of surfactant is included and the pH is 0.5 to 4.5 aqueous solution composition.

The method for preparing the composition for controlling plant viruses for foliar spraying of the present invention,

A first step of mixing and mixing zinc powder (ZnSO ₄ ·7H ₂ O 95% or more) and boron oxide powder (B ₂ O ₃ 56.45%) in distilled water (purified water), followed by stirring and immersion to form a first aqueous solution;

After mixing liquid raw materials containing 1.5 % (w/v) of nitrogen (N), 7.8 % (w/v) of phosphoric acid (P2O5), and 9.1 % (w/v) of potassium (K2O) with distilled water (purified water) a second step of stirring and immersing to form a secondary aqueous solution;

A third step of mixing the chelated copper powder (CuSO ₄ 25% or more) with the first aqueous solution and then stirring and natural immersion to form a first pure stock solution;

a fourth step of completely diluting the first pure stock solution by dropping about 2 ml/sec at a time while stirring the second aqueous solution to form a second pure stock solution;

and a fifth step of finally preparing a composition for controlling plant viruses by increasing the surfactant having an electrodeposition function in the fully formed secondary pure stock solution.

Furthermore, the present invention is a liquid plant virus control agent containing the prepared composition for controlling plant viruses as an active ingredient by treating the leaves of various Cucurbitaceous and Solanaceae crops to prevent virus movement and infection from various crops to wholesome wine, and plant growth It is a method to confirm the control effect that promotes

As a means of solving the above problems, the present invention has an antiviral effect of inhibiting virus replication and inhibiting virus movement in plants, alleviating the symptoms of a diseased strain, and inhibiting viral infection into a healthy strain through mechanical contact.

In addition, the present invention has the effect of promoting plant growth by promoting crop growth.

In addition, the present invention can improve the convenience, efficiency and economical efficiency of the control operation by treating the liquid antiviral agent on the foliage of crops.

Fig. 1 (a) is a conceptual diagram showing a general viral replication mechanism in a plant, and Fig. 1 (b) is a conceptual diagram showing the positive virus effect according to the present invention.
Figure 2 (a) is a conceptual diagram showing the general mechanism of the virus between plants, Figure 2 (b) is a conceptual diagram showing the formation of an infection barrier according to the present invention.

Terms used in this specification will be briefly described, and the present invention will be described in detail.

The terms used in the present invention have been selected as currently widely used general terms as possible while considering the functions in the present invention, but these may vary depending on the intention or precedent of a person skilled in the art, the emergence of new technology, and the like. Therefore, the term used in the present invention should be defined based on the meaning of the term and the overall content of the present invention, rather than the name of a simple term.

In the entire specification, when a part “includes” a certain component, it means that other components may be further included, rather than excluding other components, unless otherwise stated.

Hereinafter, with reference to the accompanying drawings, the embodiments of the present invention will be described in detail so that those skilled in the art can easily carry out the embodiments of the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.

Specific details including the problem to be solved for the present invention, the means for solving the problem, and the effect of the invention are included in the embodiments and drawings to be described below. Advantages and features of the present invention and methods of achieving them will become apparent with reference to the embodiments described below in detail in conjunction with the accompanying drawings.

The method for preparing the composition for controlling plant viruses for foliar spraying according to the present invention is carried out by the following steps.

First, in the first step, 2.5 parts by weight of zinc powder (ZnSO ₄ ·7H ₂ O 95% or more) and 0.9 parts by weight of boron oxide powder (B ₂ O ₃ 56.45%) based on 1 liter of distilled water (purified water) at 10-30°C When mixed (diluted), the water changes to a slightly similar color to rice water, and when it is stirred at 1,750 rpm and dissolved, bubbles with residues are generated. Stirring time should be at least 30-40 minutes, and after 8-12 hours of natural immersion, 50% of the supernatant is collected to form a primary aqueous solution. In this case, the room temperature may be a normal temperature.

If the temperature is too low or too high, self-crystals may form during the dissolution process, so it must be carried out within the given conditions. In addition, if the immersion time is less than 8 hours, floating substances remain in the supernatant and when the product is finished, a floating substance film is formed on the product, so the time must be observed.

Next, the second step is based on 1 liter of distilled water (purified water) at 10-35 ℃ nitrogen (N) 1.5% (w/v), phosphoric acid (P ₂ O ₅ ) 7.8% (w/v), potassium (K ₂ O) When 70-90 parts by weight of a liquid raw material containing 9.1% (w/v) is mixed and dissolved by stirring at a speed of 1,750 rpm for 40 to 80 minutes, a lot of bubbles are generated along with a very dark brown liquid, and the concentration increases it gets very intense If this is naturally immersed for 8 to 12 hours, a large amount of residue is generated. After that, 50-70% of the supernatant water naturally soaked is collected to make a secondary aqueous solution. The liquid raw material can be easily obtained as a liquid fertilizer in the market.

Next, in the third step, after re-warming the dissolved primary aqueous solution to 10-35°C, 150 parts by weight of chelated copper powder (CuSO4 25% or more) is mixed and stirred for 20-40 minutes at 1,750 rpm. In order to increase the synthesis rate between each other, stop all power sources and then naturally immerse for 8-12 hours while maintaining the temperature of the aqueous solution at 15-20 °C. Upon stirring, it is a clear, soft blue solution and produces white foam.

In the primary aqueous solution, copper powder cannot be completely dissolved in distilled water (purified water) at once and a lot of residue is generated. After natural immersion, the residue is completely removed through the drain pipe to form the primary pure undiluted solution.

Next, in step 4, while the already formed secondary aqueous solution is stirred at a speed of 1,750 rpm, the primary pure stock solution is dropped by about 2 ml/sec at a time, and after complete dilution, a secondary pure stock solution is formed. At this time, the temperature should be maintained at 15~30℃, and the shape shown during operation is very dark brown and has a fermented smell.

Next, in the fifth step, the fully formed secondary pure undiluted solution is uniformly well spread and electrodeposited on the surface of the crop, and the silicone surfactant is increased to facilitate penetration. The surfactant is mixed in an amount of 1.0 to 10.0% based on the final composition while stirring the secondary pure stock solution at 15 to 30° C. at a speed of 1,750 rpm.

As an aqueous solution composition through the manufacturing process of the first to fifth steps, nitrogen (N) 0.1 to 2.5%, phosphoric anhydride (P ₂ O ₅ ) 0.2 to 3.0%, potassium oxide (K2O) 0.1 to 1.0%, boron oxide (B ₂ O ₃ ) Plant virus disease for foliar spraying containing 0.05-1.5%, zinc (Zn) 0.05-3.0%, copper (Cu) 0.05-4.0% and surfactant 1.0-10.0% and pH 0.5-4.5 A composition for control is prepared.

Since the control composition contains a surfactant, it has excellent electrodeposition and diffusion functions on the plant, so that the components easily reach the virus present in the cells of the plant to stop its activity. The surfactant maintains a uniform dispersion state of the chelate compound, which is phosphoric anhydride (P2O5), even under dry or wet conditions. In addition, since the composition has excellent water affinity, ions, which are active ingredients, can be uniformly dispersed and spread throughout the plant and penetrated.

The composition for controlling plant virus disease prepared by the above preparation method preferably has a pH of 0.5 to 4.5, more preferably a pH of 0.5 to 1.5. In order for the stable pH balance of this composition to maintain plant growth and prevent virus outbreak, it must be maintained at an appropriate pH, and must be non-toxic (NON-TOXIC) to ensure safety for animals and plants. Since the proper temperature and pH are very important parts for the complete dissolution and electrodeposition of the mixed compositions, the compositions can be stably maintained by maintaining the pH of the solution in which the composition is added to 0.5 to 4.5 and stabilizing it.

As mentioned above with respect to the background of the invention, since plant viruses are obligate parasites that live in cells, there is no therapeutic agent after host infection, so direct control in the actual field was difficult. Therefore, although indirect methods such as resistant variety cultivation, seedling method, and vector source management are used, there are limitations in the immediateness, effectiveness, and efficiency of control operation. However, the plant virus control method of the present invention can be directly controlled by spraying foliage on the field during the crop cultivation period, so it can be controlled much more simply and efficiently compared to the indirect control method.

There are about 800 species in 118 genera in Cucurbitaceae crops, and most of them are annual herbaceous plants and grow in temperate climates or tropical regions (Jeffrey, 1980). Major crops belonging to the Cucurbitaceae family include watermelon (Citrullus lanatus (Thumb.) Matsum. & Nakai.), pumpkin (Cucurbita spp.), cucumber (Cucumis sativus L.), and melon (Cucumis melo L.). When looking at the global production of gourd and major crops in 2013, watermelon was 109 million tons, cucumber 71 million tons, melon 29 million tons, and pumpkin 24 million tons, accounting for about 30% of the total vegetable production (FAO, 2013) are crops that not only generate profits for farmers, but also for the seed industry. Cucumber crops have shown steady improvement in terms of quantity and quality until now due to the development of excellent varieties and cultivation techniques, but the continuous occurrence of diseases and pests is a problem.

Among the pests transmitted to gourd crops, there are more than 30 types of viruses (Povvienti, 1993), Cucumber mosaic virus (CMV) of Cucumovirus, Tomato spotted wilt virus (TSWV) of Orthotospovirus, Potyvirus Watermelon mosaic virus (WMV), Papaya ringspot virus (PRSV), Zucchni yellow mosaic virus (ZYMV), Tobamovirus genus CGMMV: Cucumber green mottle mosaic virus) are known.

In addition, it is known that there are more than 3,000 species of Solanaceae plants, which are one of the plant taxa in which speciation has occurred most diversely in the evolutionary process. These solanaceae crops contain a lot of important economic crops such as peppers, potatoes, tobacco, and tomatoes, and are used as important elements of food, spices, and medicines. Among them, tomatoes are an economically important vegetable with annual global trade of 10 trillion won.

The main viruses transmitted to Solanaceae crops include Tomato yellow leaf curl virus (TYLCV) of the genus Begomo, Tomato spotted wilt virus (TSWV) of the genus Orthotospovirus, and Cucumber: Cucumber of the genus Cucumovirus. Mosaic virus), Potyvirus genus Pepper mottle virus (PepMoV: Pepper mottle virus), etc. are known.

The composition for controlling plant virus diseases of the present invention inhibits virus replication and virus movement in plants (inhibits cell-to-cell movement of plant viruses through protoplasts), alleviates symptoms of diseased strains, and serves as a healthy liquor through mechanical contact. It has antiviral action to inhibit viral infection.

The positive viral effect exerted by the composition for controlling plant virus disease of the present invention will be described with reference to the drawings.

Fig. 1 (a) is a conceptual diagram of a general virus replication mechanism in a plant, and Fig. 1 (b) is a conceptual diagram showing the positive viral action effect of the composition for controlling a plant virus disease of the present invention to stop the activity of the virus. As shown in Fig. 1(a), virus particles generally delivered through a mediator infect a host cell, then invade the host cell nucleus, and during nuclear fission, the viral genetic material is also replicated to form new viral particles. make it Afterwards, the virus infects the entire plant as the virus particles move between cells through the protoplasmic synapses formed between the host cells. On the other hand, the composition for controlling a plant virus disease of the present invention stops the activity of the virus particles penetrating into the cytoplasm of the host cell as shown in FIG. .

The mechanism of action of the composition for controlling plant virus disease of the present invention, as shown in FIG. When a plant infected with a virus is locally treated with the composition for controlling a plant virus disease of the present invention, the activity of the virus is stopped through positive viral action, and movement through the phloem is also suppressed to prevent systemic infection.

Figure 2 (a) is a general mechanism of the virus between plants, and Figure 2 (b) shows that the infection barrier between plants is formed due to the positive effect of the composition for controlling plant virus disease of the present invention, thereby suppressing the overall virus. Since this is a mechanism that also inhibits the movement between the virus-infected strain and the uninfected healthy strain, it minimizes the spread of the virus from the infected strain to the healthy strain and spreads widely throughout the cultivated land.

The following describes examples confirming the control effect of the composition prepared by using the method for preparing the composition for controlling plant viruses for foliar spraying described above.

Example

<Materials and Methods of Control Effect Test>

The components and contents of the aqueous solution formulation for controlling plant viruses disclosed in the assay are nitrogen (N) 0.14%, phosphoric acid (P ₂ O ₅ ) 0.55%, potassium (K ₂ O) 0.64%, boron (B ₂ O ₃ ) 0.05 %, zinc (Zn) 0.05%, copper (Cu) 3.75% and surfactant 3.0% are included and the pH is 1.07 (hereinafter referred to as 'control composition product').

To test the effect of the product, a field control test was performed by selecting a representative species from among the plant virus genera that is a problem for Solanaceae and Cucurbitaceae crops (Table 1). A virus-sensitive strain was selected as the host, and tests were conducted on whole strains that were not infected with all plant diseases such as fungi, bacteria, phytoplasma, and viruses other than the target virus. All tests were conducted in a greenhouse, and no separate insect vector control was performed.

<Experimental Example 1> Tomato yellow leaf curl virus (TYLCV)

Tomato yellow leaf curl virus (TYLCV) is a genus of Begomovirus in the family Geminiviridae and causes damage in tomato plantations worldwide. The symptoms of TYLCV include yellowing of the tomato leaf edge, shrinking upward and smaller, and atrophy and growth of the entire plant (Fig. 1).

TYLCV transmission mode is directly mediated by tobacco powdery mildew, and there is no sap, seed or contact transmission. Tobacco powdery mildew can immunize with TYLCV for up to 20 days, and young plants show symptoms in 10 to 14 days.

For the control effect test of the control composition product, a package with a history of TYLCV was selected and performed. Cultivation was carried out in accordance with general customary cultivation. In the TYLCV control effect test, tomatoes were planted on August 15, referring to the report that the onset of TYLCV rapidly increased from the 20th day after planting when tomatoes were planted in August-September. The onset of TYLCV was induced naturally without separately controlling tobacco whiteflies. The test group consisted of 3 repetitions of 500-fold solution of the control composition product (pH 2.99, hereinafter the same as in other experimental examples) drug-treated group and untreated egg mass method. As for the drug treatment, the leaves were sprayed 3 times at 7-day intervals immediately after the initial TYLCV symptom was confirmed. As a result, the control value was calculated as follows by examining the morbidity rate for 50 weeks of each test group 7 days after the final drug treatment. To improve reliability, the minimum incidence rate without treatment was set at 10%. Since the symptoms of TYLCV are typical, a separate diagnosis was not performed.

15 days after formalization, the initial symptoms of TYLCV were confirmed. As a result of examining the morbidity rate of each test group 7 days after the final drug treatment, the morbidity rate of the control composition product treatment group was 6.7%, and the untreated group disease migration rate was 49.3%. .

<Experimental Example 2> Cucumber mosaic virus (CMV)

Cucumber mosaic virus (CMV) belongs to the genus Bromoviridae and Cucumovirus, and has a very wide host range of 85 families and more than 1,000 species. Peach aphids and cotton aphids are known as major vector vectors, and non-persistent transmission is achieved by the stings of these aphids. It is a plant virus that is widely distributed around the world and damages many kinds of crops, and it is one of the most important pathogens in vegetables and flowers. Plants infected with CMV mainly show mosaic symptoms, and in some plants, symptoms such as necrosis, stunting, and shoe-string appear together in the middle of the infection period.

The CMV control effect test was performed by selecting red pepper plants with a history of CMV onset. Cultivation was carried out in accordance with general customary cultivation. On April 5, 60-day-old peppers were planted in a greenhouse at 45cm intervals and naturally induced CMV without installing a mesh facility for aphid control or treating with pesticides.

The test group consisted of 3 repetitions of the 500-fold drug treatment group and the untreated egg mass method. As for the drug treatment, foliar sprays were applied 3 times at 7-day intervals immediately after the initial symptom of CMV was confirmed. As a result, the control value was calculated by the same formula as in Experimental Example 1 by examining the morbidity rate for 50 weeks of each test group 7 days after the final drug treatment. To improve reliability, the minimum incidence rate without treatment was set at 10%. CMV was diagnosed by RT-PCR.

Early symptoms of CMV were confirmed 20 days after formalization. RT-PCR was performed by randomly collecting red pepper leaves showing viral symptoms. As a result of RT-PCR, it was diagnosed as CMV, and it was confirmed that it was not infected with viruses other than CMV. The result is shown in Figure 2.

Immediately after confirming the initial symptoms, the control composition product was treated 3 times at 7-day intervals, and the disease-related illness rate was investigated 7 days after the final drug treatment. The disease transfer rate in the treatment group was 17.3% and the disease transfer rate in the untreated group was 64.7%. The typical symptoms of CMV in the untreated group are shown in Figure 4.

<Experimental Example 3> Tomato spotted wilt virus (TSWV)

Tomato spot counterfeit virus (TSWV) is a representative virus of the genus Tospoviridae and Orthotospovirus. It is known to be transmitted by 10 species of thrips, including the yellow flower thrips (Frankliniella occidentalis).

The host range is very wide and it is known to infect about 1,000 kinds of plants, and the main damaged crops are solanaceous crops such as peppers and tomatoes, legumes such as peanuts, and flowers such as chrysanthemums. Symptoms of TSWV damage include circular spots, necrotic spots, yellowing and mosaic symptoms on the leaves and fruits of crops, and in severe cases, the entire plant dies.

The main source of TSWV is thrips. The TSWV control effect test was conducted without mixing the soil pesticides for thrips larvae control by selecting red pepper fields with a history of TSWV disease. Cultivation was carried out in accordance with general customary cultivation. Red pepper seedlings that were sown for 62 days were used, and on May 2, they were planted at 45cm intervals in the greenhouse. The test group consisted of 3 repetitions of the 500-fold drug treatment group and the untreated egg mass method. As for the drug treatment, foliar sprays were applied three times at 7-day intervals immediately after confirming the initial symptoms of TSWV. As a result, the control value was calculated as in Experimental Example 1 by examining the morbidity rate for 50 weeks of each test group 7 days after the final drug treatment. To improve reliability, the minimum incidence rate without treatment was set at 10%. TSWV was diagnosed by RT-PCR.

25 days after formalization, the initial symptoms of TSWV were confirmed. RT-PCR was performed by randomly collecting red pepper leaves showing viral symptoms. As a result of RT-PCR, it was diagnosed as TSWV, and it was confirmed that it was not infected with viruses other than TSWV. The result is shown in Figure 5.

Immediately after confirming the initial symptoms, the control composition product was treated 3 times at 7-day intervals, and the disease-related illness rate was investigated 7 days after the final drug treatment. The disease transfer rate of the control composition product treated group was 21.3%, and the disease transfer rate of the untreated group was 74.7%. The typical symptoms of untreated TSWV are shown in Figure 7.

<Experimental Example 4> Cucumber green mottle mosaic virus (CGMMV)

Cucumber green mottle mosaic virus (CGMMV) is taxonomically included in the genus Virgaviidae and Tobamovirus, and has been reported to be easily transmitted by contact, mechanical inoculation, soil, and seeds.

CGMMV shows typical mosaic and malformation symptoms in plants, and in severe cases, the plants die.

For the CGMMV control effect test, melon fields where CGMMV occurs every year were conducted. Cultivation was carried out in accordance with general customary cultivation. On January 9th and 45th, seedless seedlings were planted at 35cm intervals. The test group consisted of 3 repetitions of the 500-fold drug treatment group and the untreated egg mass method. As for the drug treatment, foliar sprays were applied three times at 7-day intervals immediately after the initial symptomatic CGMMV was confirmed. As a result, the control value was calculated as in Experimental Example 1 by examining the morbidity rate for 50 weeks of each test group 7 days after the final drug treatment. To improve reliability, the minimum incidence rate without treatment was set at 10%. CGMMV was diagnosed by RT-PCR.

After 85 days of formalization, early symptoms of CGMMV were confirmed. RT-PCR was performed by randomly collecting melon leaves showing viral symptoms. As a result of RT-PCR, it was diagnosed as CGMMV, and it was confirmed that it was not infected with viruses other than CGMMV. The result for this is shown in [Figure 8].

Immediately after confirming the initial symptoms of CGMMV, the control composition product was treated 3 times at 7-day intervals, and the disease-related illness rate was investigated 7 days after the final drug treatment. The disease transfer rate of the control composition product treated group was 6.0%, and the disease transfer rate of the untreated group was 40.0%. The typical symptoms of CGMMV in the untreated group are shown in Figure 10.

In addition to the plant virus control effect test revealed in the above Examples, the present inventors confirmed the virus control effect by using the plant virus control composition of the present invention, an example tested on paprika infected with CMV (Cucumber mosaic virus), WMV ( Examples of experiments performed on zucchini complex infected with Watermelon mosaic virus and CMV (Cucumber mosaic virus), and examples of experiments with watermelon complex infected with Zucchini yellow mosaic virus (ZYMV) and Papaya ring spot virus (PRSV).

As such, those skilled in the art to which the present invention pertains will understand that the above-described technical configuration of the present invention may be implemented in other specific forms without changing the technical spirit or essential characteristics of the present invention.

Therefore, the embodiments described above are to be understood as illustrative and not restrictive in all respects, and the scope of the present invention is indicated by the following claims rather than the above detailed description, and the meaning and scope of the claims and their All changes or modifications derived from the concept of equivalents should be construed as being included in the scope of the present invention.

Claims (5)

As an aqueous solution composition, nitrogen (N) 0.14%, phosphoric anhydride (P ₂ O ₅ ) 0.55%, potassium oxide (K ₂ O) 0.64%, boron oxide (B ₂ O ₃ ) 0.05%, zinc (Zn) 0.05%, Copper (Cu) 3.75% and surfactant 3.0% A composition for controlling plant viruses of Cucurbitaceae crops or Solanaceae crops for foliage spraying, characterized in that it contains 3.75% and 3.0% surfactant. According to claim 1, wherein the pH of the composition for controlling plant viruses is 0.5 to 4.5, the composition for controlling plant viruses of the solanaceae crops or gourds for leaf spraying, characterized in that. A method for controlling plant viruses of Cucurbitaceae crops or Solanaceae crops by spraying the composition for controlling plant viruses of claim 1 on the foliage of plants. According to claim 3, wherein the plant virus is tomato yellow leaf curl virus (TYLCV), cucumber mosaic virus (CMV), tomato spotted wilt virus (TSWV), cucumber Consists of Cucumber green mottle mosaic virus (CGMMV), watermelon mosaic virus (WMV), Zucchini yellow mosaic virus (ZYMV) and Papaya ringspot virus (PRSV) A method for controlling plant viruses of gourds or Solanaceae crops, characterized in that at least one selected from the group. As a method for preparing the composition for controlling plant viruses of claim 1,
Zinc powder (ZnSO ₄ ·7H ₂ O 95% or more) and boron oxide powder (B ₂ O ₃ 56.45%) are administered and mixed in distilled water (purified water) at 10-30°C and stirred at 1,750 rpm for 30-40 minutes a first step of forming a first aqueous solution with 50% of the supernatant water collected by dissolving and immersing naturally for 8 to 12 hours;
Based on 1 liter of distilled water (purified water) at 10-35℃, nitrogen (N) 1.5 % (w/v), phosphoric acid (P ₂ O ₅ ) 7.8 % (w/v), potassium (K ₂ O) 9.1 % (w) After mixing 70 to 90 parts by weight of liquid raw material containing second step;
After reheating the first aqueous solution to 10 ~ 35 ℃, 150 parts by weight of chelated copper powder (CuSO ₄ 25% or more) was mixed and stirred at a speed of 1,750 rpm for 20 to 40 minutes, and then to increase the synthesis rate between formulations Naturally immerse for 8-12 hours while maintaining the temperature of the aqueous solution at 15-20℃, remove some residue through the drain pipe, stir again at 1,750 rpm for 20-40 minutes, immerse naturally for 8-12 hours, and then pass through the drain pipe. A third step of completely removing the residue to form a primary pure stock solution;
a fourth step of completely diluting the primary pure undiluted solution by dropping 2 ml/sec at a time while maintaining the temperature at 15-30° C. while stirring the secondary aqueous solution at a speed of 1,750 rpm to form a secondary pure undiluted solution;
and a fifth step of increasing the silicone surfactant in the fully formed secondary pure stock solution.

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