KR102436573B1 - Senior high protein oral composition comprising radish leaves extracts and the process for the preparation thereof - Google Patents
Senior high protein oral composition comprising radish leaves extracts and the process for the preparation thereof Download PDFInfo
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- KR102436573B1 KR102436573B1 KR1020210030521A KR20210030521A KR102436573B1 KR 102436573 B1 KR102436573 B1 KR 102436573B1 KR 1020210030521 A KR1020210030521 A KR 1020210030521A KR 20210030521 A KR20210030521 A KR 20210030521A KR 102436573 B1 KR102436573 B1 KR 102436573B1
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- A—HUMAN NECESSITIES
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Abstract
Description
본 발명은 무청 추출액을 유효성분으로 함유하는 노인식 고단백 경구용 조성물 및 이의 제조방법에 대한 것으로, 보다 상세하게는 무청 열수 추출액과 함께 쌀 추출액, 대두 단백질, 프락토 올리고당, 알룰로오스 및 비타민 C와 비타민 B의 혼합제제를 유효성분으로 포함하는 것을 특징으로 하는 노인식 고단백 경구용 조성물로서 식이섬유, 칼슘, Fe, 총 폴리페놀, 유리아미노산, 무기질 등 노인에게 유용한 기능성 성분이 다량 함유되어 있을 뿐만 아니라 기호도가 증대된 노인식 고단백 경구용 조성물 및 이의 제조방법에 관한 것이다.The present invention relates to a composition for oral administration of high protein for the elderly containing a radish extract as an active ingredient and a method for preparing the same, and more particularly, to a composition for oral administration of a high-protein composition for the elderly containing a radish extract as an active ingredient. A high-protein oral composition for the elderly, characterized in that it contains a vitamin B mixture as an active ingredient. The present invention relates to a composition for oral high-protein oral administration for the elderly, and a method for preparing the same.
우리나라의 2013년 현재 총인구는 50,142,158명이며, 이 중 65세~74세 노인인구는 3,658,353명, 75세~84세 노인인구는 2,007,685, 85세 이상 노인인구는 471,664명으로 집계되었다. 65세 이상 노인인구는 전체 인구의 11%로 고령화 사회로 매우 빠른 속도로 진입하고 있다. 이처럼 현재 우리나라는 가장 급속하게 고령화가 진행되고 있으며 2018년에는 노인인구 비율이 14.3%로 고령사회로 진입이 예상되며 2026년에는 20.8%에 도달하여 초고령 사회로의 진입이 예상되고 있다. 급격한 노인인구의 증가에 따라 노인문제가 점차 사회문제로 인식되고 있으며 노인이 건강하게 장수하기 위해서는 신체적, 정신적 건강과 더불어 균형 잡힌 영양섭취가 필수적이라고 할 수 있다. 노인의 건강은 영양 상태와 밀접한 관련이 있으며 생리적, 사회적, 경제적, 심리적인 요인으로 인해 식품 선택에 제한이 생기게 되며, 더 나아가 영양불량이 초래된다. 노화에 의해 저작, 소화, 흡수 등의 기능 저하가 일어나며 치아의 부실 또는 손실은 음식물의 저작에 영향을 미쳐 영양 불량이나 편식을 초래할 수 있다. 또한 나이가 들수록 후각과 미각의 기능이 감소하여 맛을 즐기지 못해 음식의 섭취가 감소하고 영양흡수와 영양소 사용이 훼손되어 평균 인구와 비교하였을 때 영양부족에 대한 위험도가 증가하게 된다.As of 2013, the total population of Korea is 50,142,158, of which 3,658,353 people aged 65 to 74 years, 2,007,685 people aged 75 to 84, and 471,664 people aged 85 or older. The elderly population over the age of 65 accounts for 11% of the total population, and is rapidly entering an aging society. As such, Korea is currently aging the most rapidly, and in 2018, the proportion of the elderly population is 14.3%, which is expected to enter an aged society, and in 2026, it is expected to reach 20.8% and enter a super-aged society. With the rapid increase of the elderly population, the elderly problem is increasingly recognized as a social problem, and in order for the elderly to live a long and healthy life, it can be said that a balanced nutritional intake along with physical and mental health is essential. The health of the elderly is closely related to their nutritional status, and physiological, social, economic, and psychological factors cause restrictions on food choices, which further leads to malnutrition. Aging causes deterioration of functions such as mastication, digestion, and absorption, and tooth loss or loss can affect chewing of food, resulting in poor nutrition or unbalanced diet. In addition, as the function of smell and taste decreases with age, food intake decreases due to the inability to enjoy taste, and nutrient absorption and nutrient use are impaired, which increases the risk of malnutrition compared to the average population.
노인에게 원기를 회복시킬 수 있는 음식과 영양 제공, 먹는 것에 대한 심리적인 요소를 고려한 건강한 영양 상태는 삶의 질과 밀접한 관계가 있다. 노인의 균형 잡힌 영양섭취는 노인의 삶을 보다 건강하게 누릴 수 있게 해주는 수단이다. 따라서 노인들이 건강한 노년을 보내기 위해서는 무엇보다 균형 잡힌 노인식의 개발은 필수적이라 볼 수 있다. 음식을 먹는 행위는 인생 최대의 즐거움이라고 말할 수 있지만 먹는 행위에 관여하는 기능에 장애가 있으면 먹는 즐거움을 잃게 될 뿐만 아니라 저영양 상태에 직면하게 되며 생명에도 위해를 미칠 수 있다. 연하, 저작 장애는 다양한 질환으로 생기지만 고령도 큰 원인의 하나이다. 고령에 따라 연하, 저작, 소화 흡수 등의 신체 기능이 저하되어 가며 앞으로 기능이 저하된 고령자는 급증할 것으로 예상된다. 이러한 상황에서 연하, 저작 등을 배려한 노인식의 개발이 요구된다.Healthy nutrition is closely related to the quality of life, considering the psychological factors of providing the elderly with rejuvenating food and nutrition, and eating. A balanced nutrition intake for the elderly is a means to enable them to enjoy a healthier life. Therefore, it is essential to develop a balanced diet for the elderly to live a healthy old age. It can be said that the act of eating is the greatest pleasure in life, but if there is a dysfunction in the function involved in the act of eating, not only will you lose the pleasure of eating, but you will also face a low-nutrition state and it can be life-threatening. Swallowing and mastication disorders are caused by various diseases, but aging is also one of the major causes. With aging, body functions such as swallowing, mastication, digestion and absorption decline, and the number of elderly people with reduced functions is expected to increase sharply in the future. In such a situation, it is required to develop a diet for the elderly in consideration of swallowing and writing.
한편, 무(Raphanus sativus L.)는 고려시대부터 재배가 보편화된 것으로 추정되는 우리나라 대표적인 채소로 쌍떡잎식물, 양귀비목, 겨자과에 속하는 한해살이 또는 두해살이 초본으로, 수분 약 93%, 조단백질 1%, 당질 약 3%, 비타민 C 19~39 mg%를 함유하고 있다. 무의 조직에는 섬유소와 펙틴질, 각종 무기질과 소화효소인 amylase 등이 있으며, methyl mercaptane이나 mustard oil과 같은 특유의 방향성분을 가지고 있다. On the other hand, radish (Raphanus sativus L.) is a representative vegetable in Korea that is estimated to have been cultivated universally since the Goryeo Dynasty. It is an annual or biennial herb belonging to the dicotyledonous plant, Papaver, and Mustard family. It contains about 93% of moisture, 1% of crude protein, and about approx. It contains 3%, vitamin C 19-39 mg%. Radish tissue contains fibrin, pectin, various minerals, and amylase, a digestive enzyme, and has unique aromatic components such as methyl mercaptane and mustard oil.
또한, 무청(Radish leaves)은 무의 지상부 엽채를 말하며 우리나라에서는 건조하여 국이나 나물로 조리하여 이용하며, 영양학적으로 비타민 A와 C, 칼슘이 뿌리 부분에 비하여 더 많이 함유되어 있고, 건조된 무청(시래기)은 풍부한 식이섬유의 주요 급원으로 알려지면서 무청의 효율적인 이용에 대한 관심이 증가되고 있으나, 이러한 우수성에도 불구하고 재배되는 무의 소비량에 비해 무청의 소비율이 적어 많은 양의 무청이 폐기되고 있어 적절한 대안이 필요한 실정이다.In addition, radish leaves refer to the above-ground part of radish. In Korea, it is dried and cooked with soup or vegetables. Nutritionally, it contains more vitamins A, C, and calcium than the root part, and dried radish leaves. (Siraegi) is known as a major source of abundant dietary fiber, and interest in the efficient use of radish is increasing. Appropriate alternatives are needed.
현재 무청에 대한 연구로는 조리방법에 따른 미네랄 함량 변화, 열풍건조에 따른 품질 특성 변화, 무청의 건조방법에 따른 미생물 변화 등이 알려져 있으며, 생리활성에 대한 보고로는 무청의 항고혈압 활성, 유방암 세포 성장억제활성, 폐암세포 생육억제효과, 항산화 활성, 콜레스테롤 축적 억제효과, 장 기능 개선 및 혈중 지질 개선 효과, 위 장 자극 저하 및 자궁수축 활성 등이 있다. Current research on radish includes changes in mineral content according to cooking methods, changes in quality characteristics due to hot air drying, and microbial changes according to drying methods of radish. It has cell growth inhibitory activity, lung cancer cell growth inhibitory effect, antioxidant activity, cholesterol accumulation inhibitory effect, intestinal function improvement and blood lipid improvement effect, gastrointestinal stimulation lowering and uterine contraction activity.
이에 본 발명자들은 노인식 경구용 기능성 식품을 개발하기 위해 계속 연구를 진행하던 중 무청 추출액을 이용하여 제조된 경구용 조성물이 식이섬유, 칼슘, Fe, 총 폴리페놀, 유리아미노산, 무기질 등 노인에게 유용한 기능성 성분이 다량 함유되어 있을 뿐만 아니라 노인에 대한 기호도도 증대시킬 수 있다는 사실을 발견함으로써 본 발명을 완성하였다.Therefore, the present inventors continued research to develop functional food for the elderly, and while the study was continued, an oral composition prepared using a non-corrosive extract was useful for the elderly such as dietary fiber, calcium, Fe, total polyphenols, free amino acids, minerals, etc. The present invention was completed by discovering the fact that not only contains a large amount of ingredients, but also increases the preference for the elderly.
따라서, 본 발명에서 해결하고자 하는 기술적 과제는 노인식 고단백 경구용 조성물을 제공하기 위한 것이다. Accordingly, the technical problem to be solved by the present invention is to provide a composition for oral use with high protein for the elderly.
또한, 본 발명에서 해결하고자 하는 다른 기술적 과제는 노인식 고단백 경구용 조성물의 제조방법을 제공하기 위한 것이다. In addition, another technical problem to be solved in the present invention is to provide a method for preparing a composition for oral administration of high protein for the elderly.
상기한 기술적 과제를 해결하기 위하여, 본 발명에서는 무청 추출액을 유효성분으로 포함하는 노인식 고단백 경구용 조성물을 제공한다.In order to solve the above technical problem, the present invention provides a composition for oral administration of high protein for the elderly comprising a radish extract as an active ingredient.
바람직하게, 상기 노인식 고단백 경구용 조성물은 무청 추출액을 0.1 내지 10 중량%의 양으로 포함하는 것을 특징으로 한다.Preferably, the composition for oral administration of high protein for the elderly is characterized in that it comprises 0.1 to 10% by weight of the green radish extract.
바람직하게, 상기 무청 추출액은 냉동 저장한 무청을 해동하여 탈수한 후 10 내지 14시간 동안 열풍 건조한 후 분쇄하여 15 내지 25배(부피비)의 증류수를 가하여 90 내지 110℃에서 5 내지 7 시간 동안 열수 추출하여 수득된 것임을 특징으로 한다.Preferably, the radish extract is thawed and dehydrated, dried in hot air for 10 to 14 hours, then pulverized and 15 to 25 times (volume ratio) of distilled water is added to extract hot water at 90 to 110° C. for 5 to 7 hours. It is characterized in that it has been obtained.
바람직하게, 상기 추출공정에서 열수 추출 시 상기 무청 분말의 총 중량을 기준으로 하여 2 내지 5 중량% 양의 구연산을 첨가하여 추출하는 것을 특징으로 한다.Preferably, it is characterized in that the extraction is performed by adding citric acid in an amount of 2 to 5% by weight based on the total weight of the rust-free powder during hot water extraction in the extraction process.
바람직하게, 상기 노인식 고단백 경구용 조성물은 쌀 추출액, 대두 단백질, 프락토 올리고당, 알룰로오스 및 비타민 C와 비타민 B가 97:3으로 혼합된 비타민 혼합제제를 추가로 포함하는 것을 특징으로 한다.Preferably, the composition for oral administration of high protein for the elderly is characterized in that it further comprises a vitamin mixture in which rice extract, soy protein, fructooligosaccharide, allulose, and vitamin C and vitamin B are mixed in a ratio of 97:3.
상기 쌀 추출액 5 내지 10 중량%, 대두 단백질 3 내지 8 중량%, 프락토 올리고당 1 내지 3 중량%, 알룰로오스 1 내지 5 중량% 및 비타민 혼합제제 0.02 내지 0.05 중량%의 양으로 포함하는 것이 바람직하다.It is preferable to include 5 to 10% by weight of the rice extract, 3 to 8% by weight of soy protein, 1 to 3% by weight of fructooligosaccharide, 1 to 5% by weight of allulose, and 0.02 to 0.05% by weight of the vitamin mixture. do.
상기한 다른 기술적 과제를 해결하기 위하여, 본 발명에서는 하기 단계를 포함하는 것을 특징으로 하는 무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 제조방법을 제공한다:In order to solve the other technical problems described above, the present invention provides a method for preparing a composition for oral administration of high protein for the elderly comprising the following steps:
(S1) 냉동 저장한 무청을 해동하여 탈수한 후 10 내지 14시간 동안 열풍 건조한 후 분쇄하여 15 내지 25배(부피비)의 증류수를 가하여 90 내지 110℃에서 5 내지 7 시간 동안 열수 추출하여 무청 추출액을 수득하는 단계;(S1) After thawing and dehydration of frozen radish, dried with hot air for 10 to 14 hours, pulverized, added 15 to 25 times (volume ratio) of distilled water, and hot water extraction at 90 to 110 ° C. for 5 to 7 hours to obtain a rust free extract obtaining;
(S2) 상기 단계 (S1)에서 제조된 무청 추출액 0.1 내지 10 중량%에 쌀 추출액 5 내지 10 중량%, 대두 단백질 3 내지 8 중량%, 프락토 올리고당 1 내지 3 중량%, 알룰로오스 1 내지 5 중량% 및 비타민 혼합제제 0.02 내지 0.05 중량% 및 잔량의 물을 혼합하는 단계;(S2) 5 to 10% by weight of rice extract, 3 to 8% by weight of soybean protein, 1 to 3% by weight of fructose oligosaccharide, 1 to 5% by weight of allulose in 0.1 to 10% by weight of the unsweetened extract prepared in step (S1) mixing the weight% and 0.02 to 0.05% by weight of the vitamin mixture and the remaining amount of water;
(S3) 상기 단계 (S2)에서 수득된 혼합물을 90 내지 110℃에서 2 내지 5분 동안 가열하는 단계; 및(S3) heating the mixture obtained in step (S2) at 90 to 110° C. for 2 to 5 minutes; and
(S4) 상기 단계 (S3)에서 가열된 혼합물을 실온에서 25 내지 35 분간 냉각한 다음 4℃에서 냉장시켜 무청 추출액을 함유하는 노인식 고단백 경구용 조성물을 수득하는 단계.(S4) cooling the mixture heated in step (S3) at room temperature for 25 to 35 minutes, and then refrigeration at 4° C. to obtain a composition for oral use with high protein content for the elderly containing a rust-free extract.
이와 같이, 본 발명에 따른 무청 추출액을 유효성분으로 포함하는 노인식 고단백 경구용 조성물은 식이섬유, 칼슘, Fe, 총 폴리페놀, 유리아미노산, 무기질 등 노인에게 유용한 기능성 성분이 다량 함유되어 있다. 또한, 본 발명의 노인식 고단백 경구용 조성물은 색, 맛 및 풍미에 대한 평가가 우수하여 전체적인 기호도가 대폭 증대되어 노인식 고단백 기능성 경구용 식품으로 매우 유용하게 이용될 수 있을 것으로 기대된다.As described above, the composition for oral administration of high protein for the elderly comprising the radish extract according to the present invention as an active ingredient contains a large amount of functional ingredients useful for the elderly, such as dietary fiber, calcium, Fe, total polyphenols, free amino acids, and minerals. In addition, the composition for oral use with high protein for the elderly of the present invention is expected to be very useful as an oral high-protein functional food for the elderly because it has excellent evaluation of color, taste and flavor, and thus overall preference is greatly increased.
본 명세서에 첨부되는 다음의 도면들은 본 발명의 바람직한 실시예를 예시하는 것이며, 전술한 발명의 내용과 함께 본 발명의 기술사상을 더욱 이해시키는 역할을 하는 것이므로, 본 발명은 그러한 도면에 기재된 사항에만 한정되어 해석되어서는 아니 된다.
도 1은 무청 추출액의 제조공정을 도식화한 것이다.
도 2는 무청 추출액 제조공정을 보여주는 각 단계별 사진이다.
도 3은 본 발명에 따라 제조된 무청 추출액 A 및 무청 추출액 B의 사진이다.
도 4는 본 발명에 따른 무청 추출액 함유 노인식 고단백 경구용 조성물의 제조공정을 도식화한 것이다.
도 5는 본 발명에 따라 제조된 무청 추출액 함유 노인식 고단백 경구용 조성물이 포장된 상품의 일 례를 보여주는 것이다.The following drawings attached to the present specification illustrate preferred embodiments of the present invention, and serve to further understand the technical idea of the present invention together with the above-described contents of the present invention, so the present invention is limited to the matters described in such drawings It should not be construed as being limited.
1 is a schematic diagram of the manufacturing process of a rust free extract.
Figure 2 is a photograph showing each step of the manufacturing process of the extract without rust.
Figure 3 is a photograph of the non-corrosive extract A and the non-corrosive extract B prepared according to the present invention.
Figure 4 is a schematic diagram of the manufacturing process of the composition for oral use of the elderly food containing a radish extract according to the present invention.
5 shows an example of a packaged product in which the elderly food high-protein oral composition containing radish extract prepared according to the present invention is packaged.
이하 본 발명을 좀더 상세하게 설명한다.Hereinafter, the present invention will be described in more detail.
본 발명에서는 무청 추출액을 유효성분으로 포함하는 것을 특징으로 하는 노인식 고단백 경구용 조성물을 제공한다.In the present invention, there is provided a composition for oral use with high protein for the elderly, characterized in that it contains a radish extract as an active ingredient.
본 발명의 노인식 고단백 경구용 조성물의 유효성분인 무청(Radish leaves)은 무의 지상부 엽채로서, 영양학적으로 비타민 A와 C, 칼슘이 함유되어 있고, 풍부한 식이섬유를 함유하고 있으며, 항고혈압 활성, 유방암 세포 성장억제활성, 폐암세포 생육억제효과, 항산화 활성, 콜레스테롤 축적 억제효과, 장 기능 개선 및 혈중 지질 개선 효과, 위 장 자극 저하 및 자궁수축 활성 등이 있다. Radish leaves, which are the active ingredients of the composition for oral administration of high protein for the elderly of the present invention, are above-ground leaves of radishes, nutritionally contain vitamins A and C, and calcium, contain abundant dietary fiber, and have antihypertensive activity, It has breast cancer cell growth inhibitory activity, lung cancer cell growth inhibitory effect, antioxidant activity, cholesterol accumulation inhibitory effect, intestinal function improvement and blood lipid improvement effect, gastrointestinal stimulation lowering and uterine contraction activity.
본 발명의 용어 "추출액(extract)"은 무청을 적절한 침출액으로 짜내고 침출액을 증발시켜 농축한 제제를 의미하는 것으로, 이에 제한되지는 않으나, 추출처리에 의해 얻어지는 추출액, 추출액의 희석액 또는 농축액, 추출액을 건조하여 얻어지는 건조물, 이들의 조정제물 또는 정제물일 수 있다. As used herein, the term "extract" refers to a preparation obtained by squeezing radish greens with an appropriate leachate and evaporating the leachate, but is not limited thereto, and includes, but is not limited to, an extract obtained by extraction, a diluted or concentrated liquid of the extract, or an extract. It may be a dried product obtained by drying, a crude product thereof, or a purified product.
본 발명의 무청 추출액은 당업계에 공지된 일반적인 추출방법, 분리 및 정제방법을 이용하여 제조할 수 있다. 상기 추출방법으로는 열탕 추출, 열수 추출, 냉침 추출, 환류 냉각 추출 또는 초음파 추출 등의 방법을 사용할 수 있으나, 이에 제한되지는 않는다. The rust free extract of the present invention can be prepared using a general extraction method, separation and purification method known in the art. As the extraction method, a method such as hot water extraction, hot water extraction, cold extraction, reflux cooling extraction, or ultrasonic extraction may be used, but is not limited thereto.
본 발명의 하나의 구현예에 따르면, 상기 무청 추출액은 무를 그대로 분쇄하여 사용하거나, 열풍건조기 또는 동결건조기를 이용하여 건조하여 분쇄한 후 추출용매, 예컨대 물, 탄소수 1~3개의 무수 또는 함수 저급 알콜 및 이들의 혼합용매로 이루어진 군에서 선택된 용매를 추출용매, 바람직하게는 물 또는 에탄올을 사용하여 추출하여 수득할 수 있다. 구체적으로, 상기 무청 추출액은 냉동 저장한 무청을 해동하여 탈수한 후 10 내지 14시간, 바람직하게는 12시간 동안 열풍 건조한 후 분쇄하여 15 내지 25배(부피비), 바람직하게는 20배(부피비)의 증류수를 가하여 90 내지 110℃, 바람직하게는 100℃에서 5 내지 7 시간, 바람직하게는 6 시간 동안 열수 추출하여 수득할 수 있다. According to one embodiment of the present invention, the non-corrosive extract is used by pulverizing radish as it is, or after drying and pulverizing using a hot air dryer or freeze dryer, an extraction solvent such as water, anhydrous or hydrous lower alcohol having 1 to 3 carbon atoms And a solvent selected from the group consisting of a solvent mixture thereof can be obtained by extraction using an extraction solvent, preferably water or ethanol. Specifically, the radish extract is dried by hot air for 10 to 14 hours, preferably 12 hours, after thawing and dehydrating frozen radish extract, and then pulverized to 15 to 25 times (volume ratio), preferably 20 times (volume ratio). It can be obtained by hot water extraction at 90 to 110° C., preferably 100° C. for 5 to 7 hours, preferably 6 hours by adding distilled water.
본 발명의 하나의 구현예에 따르면, 상기 무청 추출액을 추가로 농축하여 30 내지 40 °Brix를 가지는 것을 특징으로 한다.According to one embodiment of the present invention, it is characterized in that it has 30 to 40 °Brix by further concentrating the extract without rust.
본 발명의 하나의 구현예에 따르면, 상기 추출공정에서 열수 추출 시 상기 무청 분말의 총 중량을 기준으로 하여 2 내지 5 중량% 양의 구연산을 첨가하여 추출함으로써 식이섬유가 다량 함유된 무청 추출액을 수득할 수 있다.According to one embodiment of the present invention, when extracting with hot water in the extraction process, citric acid in an amount of 2 to 5% by weight is added based on the total weight of the non-corrosive powder, thereby obtaining a non-corrosive extract containing a large amount of dietary fiber. can do.
본 발명의 하나의 구현예에 따르면, 본 발명의 노인식 고단백 경구용 조성물은 무청 추출액을 0.1 내지 10 중량%, 바람직하게는 0.5 내지 5 중량%의 양으로 포함하는 것을 특징으로 한다.According to one embodiment of the present invention, the composition for oral administration of high protein for the elderly of the present invention is characterized in that it comprises 0.1 to 10% by weight of the extract, preferably 0.5 to 5% by weight.
본 발명의 하나의 구현예에 따르면, 본 발명의 노인식 고단백 경구용 조성물은 쌀 추출액, 대두 단백질, 프락토 올리고당, 알룰로오스 및 비타민 C와 비타민 B가 97:3으로 혼합된 비타민 혼합제제를 추가로 포함하는 것을 특징으로 한다.According to one embodiment of the present invention, the composition for oral administration of high protein for the elderly of the present invention adds a vitamin mixed preparation in which rice extract, soy protein, fructooligosaccharide, allulose, and vitamin C and vitamin B are mixed in a ratio of 97:3 It is characterized in that it contains.
상기 쌀 추출액 5 내지 10 중량%, 대두 단백질 3 내지 8 중량%, 프락토 올리고당 1 내지 3 중량%, 알룰로오스 1 내지 5 중량% 및 비타민 혼합제제 0.02 내지 0.05 중량%의 양으로 포함하는 것이 바람직하다.It is preferable to include 5 to 10% by weight of the rice extract, 3 to 8% by weight of soy protein, 1 to 3% by weight of fructooligosaccharide, 1 to 5% by weight of allulose, and 0.02 to 0.05% by weight of the vitamin mixture. do.
본 발명의 하나의 구현예에 따르면, 본 발명에 따른 무청 추출액을 유효성분으로 포함하는 노인식 고단백 경구용 조성물은 식이섬유, 칼슘, Fe, 총 폴리페놀, 유리아미노산, 무기질 등 유용한 기능성 성분이 다량 함유되어 있다. According to one embodiment of the present invention, the composition for oral use of high protein for the elderly comprising the green radish extract according to the present invention as an active ingredient contains a large amount of useful functional ingredients such as dietary fiber, calcium, Fe, total polyphenols, free amino acids, and minerals. has been
본 발명의 하나의 구현예에 따르면, 본 발명에 따른 노인식 고단백 경구용 조성물은 본 발명의 목적효과에 영향을 주지 않는 범위 내에서 맛과 향을 향상시키기 위하여 추가의 첨가제를 첨가할 수 있다. 예를 들어, 향료, 당성분 등을 추가로 포함할 수 있다. According to one embodiment of the present invention, to the composition for oral administration of high protein for the elderly according to the present invention, additional additives may be added to improve taste and flavor within a range that does not affect the intended effect of the present invention. For example, it may further include a fragrance, a sugar component, and the like.
상기 향료는 노인식 고단백 경구용 조성물의 관능적 요소 중 풍미를 증진시키기 위해 사용한다. 향료로는 천연향료, 합성향료 등을 사용할 수 있다. 노인식 고단백 경구용 조성물 중의 향료 함량은 0.05 내지 5.0 중량%로 하는 것이 바람직한데, 함량이 0.05 중량% 미만이면 풍부한 향을 제공하지 못하여 관능적으로 좋은 결과를 얻을 수 없는 문제가 있을 수 있고, 5.0 중량%를 초과하면 노인식 고단백 경구용 조성물이 연화되고 관능적 평가에서 좋은 결과를 얻을 수 없다.The fragrance is used to enhance flavor among the sensory elements of the composition for oral administration of high protein for the elderly. As the fragrance, natural fragrances, synthetic fragrances, etc. may be used. It is preferable that the content of fragrance in the composition for oral administration of high protein for the elderly is 0.05 to 5.0% by weight, but if the content is less than 0.05% by weight, there may be a problem in that it cannot provide a rich flavor and thus good results cannot be obtained sensually, 5.0% by weight If it is exceeded, the high-protein oral composition for the elderly is softened and good results cannot be obtained in sensory evaluation.
상기 당성분은 노인식 고단백 경구용 조성물의 단맛을 증진시키기 위해 사용된다. 당성분으로는 설탕, 액상과당, 꿀 등을 사용할 수 있다. 노인식 고단백 경구용 조성물 중의 당성분 함량은 1 내지 30 중량%로 하는 것이 바람직한데, 함량이 1 중량% 미만이면 단맛을 제공하지 못하여 관능적으로 좋은 결과를 얻을 수 없는 문제가 있을 수 있고, 30 중량%를 초과하면 단맛이 증가하여 관능적으로 좋지 않은 문제가 있다.The sugar component is used to enhance the sweetness of the high-protein oral composition for the elderly. Sugar, liquid fructose, honey, etc. may be used as the sugar component. It is preferable that the content of sugar components in the composition for oral administration of high protein for the elderly is 1 to 30% by weight, but if the content is less than 1% by weight, there may be a problem in that it does not provide a sweet taste and thus good results cannot be obtained sensibly, and 30% by weight If it is exceeded, there is a problem that the sweetness is increased, which is not good sensibly.
한편, 본 발명에서는 하기 단계를 포함하는 것을 특징으로 하는 무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 제조방법을 제공한다:On the other hand, the present invention provides a method for preparing a composition for oral use with a high protein composition for the elderly comprising the following steps:
(S1) 냉동 저장한 무청을 해동하여 탈수한 후 10 내지 14시간 동안 열풍 건조한 후 분쇄하여 15 내지 25배(부피비)의 증류수를 가하여 90 내지 110℃에서 5 내지 7 시간 동안 열수 추출하여 무청 추출액을 수득하는 단계;(S1) After thawing and dehydration of frozen radish, dried with hot air for 10 to 14 hours, pulverized, added 15 to 25 times (volume ratio) of distilled water, and hot water extraction at 90 to 110 ° C. for 5 to 7 hours to obtain a rust free extract obtaining;
(S2) 상기 단계 (S1)에서 제조된 무청 추출액 0.1 내지 10 중량%에 쌀 추출액 5 내지 10 중량%, 대두 단백질 3 내지 8 중량%, 프락토 올리고당 1 내지 3 중량%, 알룰로오스 1 내지 5 중량% 및 비타민 혼합제제 0.02 내지 0.05 중량% 및 잔량의 물을 혼합하는 단계;(S2) 5 to 10% by weight of rice extract, 3 to 8% by weight of soybean protein, 1 to 3% by weight of fructose oligosaccharide, 1 to 5% by weight of allulose in 0.1 to 10% by weight of the unsweetened extract prepared in step (S1) Mixing the weight% and 0.02 to 0.05% by weight of the vitamin mixture and the remaining amount of water;
(S3) 상기 단계 (S2)에서 수득된 혼합물을 90 내지 110℃에서 2 내지 5분 동안 가열하는 단계; 및(S3) heating the mixture obtained in step (S2) at 90 to 110° C. for 2 to 5 minutes; and
(S4) 상기 단계 (S3)에서 가열된 혼합물을 실온에서 25 내지 35 분간 냉각한 다음 4℃에서 냉장시켜 무청 추출액을 함유하는 노인식 고단백 경구용 조성물을 수득하는 단계.(S4) cooling the mixture heated in step (S3) at room temperature for 25 to 35 minutes, and then refrigeration at 4° C. to obtain a composition for oral use with high protein content for the elderly containing a rust-free extract.
본 발명의 하나의 구현예에 따르면, 상기 단계 (S1)의 무청 추출액의 추출공정에서 열수 추출 시 상기 무청 분말의 총 중량을 기준으로 하여 2 내지 5 중량% 양의 구연산을 첨가하여 추출하는 것을 특징으로 한다.According to one embodiment of the present invention, the extraction is performed by adding citric acid in an amount of 2 to 5% by weight based on the total weight of the rustless powder during hot water extraction in the extraction process of the rustless extract of step (S1). do it with
본 발명의 하나의 구현예에 따르면, 상기 단계 (S1)의 무청 추출액의 추출공정에서 수득된 무청 추출액을 추가로 농축하는 공정을 거쳐 30 내지 40 °Brix의 무청 추출액을 수득하는 것을 특징으로 한다.According to one embodiment of the present invention, through a process of further concentrating the rustless extract obtained in the extracting process of the rustless extract of the step (S1), it is characterized in that 30 to 40 °Brix of the rustless extract is obtained.
이와 같이, 본 발명에 따른 무청 추출액을 유효성분으로 포함하는 노인식 고단백 경구용 조성물은 식이섬유, 칼슘, Fe, 총 폴리페놀, 유리아미노산, 무기질 등 노인에게 유용한 기능성 성분이 다량 함유되어 있다. 또한, 본 발명의 노인식 고단백 경구용 조성물은 색, 맛 및 풍미에 대한 평가가 우수하여 전체적인 기호도가 대폭 증대되어 노인식 고단백 기능성 경구용 식품으로 매우 유용하게 이용될 수 있을 것으로 기대된다.As described above, the composition for oral administration of high protein for the elderly comprising the radish extract according to the present invention as an active ingredient contains a large amount of functional ingredients useful for the elderly, such as dietary fiber, calcium, Fe, total polyphenols, free amino acids, and minerals. In addition, the composition for oral use with high protein for the elderly of the present invention is expected to be very useful as an oral high-protein functional food for the elderly because it has excellent evaluation of color, taste and flavor, and thus overall preference is greatly increased.
이하, 본 발명의 이해를 돕기 위하여 실시예 등을 들어 상세하게 설명하기로 한다. 그러나, 본 발명에 따른 실시예들은 여러 가지 다른 형태로 변형될 수 있으며, 본 발명의 범위가 하기 실시예들에 한정되는 것으로 해석되어서는 안 된다. 본 발명의 실시예들은 당업계에서 평균적인 지식을 가진 자에게 본 발명을 보다 완전하게 설명하기 위해 제공되는 것이다. Hereinafter, examples and the like will be described in detail to help the understanding of the present invention. However, the embodiments according to the present invention may be modified in various other forms, and the scope of the present invention should not be construed as being limited to the following examples. The embodiments of the present invention are provided to more completely explain the present invention to those of ordinary skill in the art.
<실시예 1> 무청 추출액 제조<Example 1> Preparation of extract without rust
가. 실험 재료 및 방법go. Experimental materials and methods
(1) 재료(1) Material
본 실험에서 사용한 무청은 충북 청주지역에서 구매한 국내산 무청을 세척한 후 냉동 보관하면서 시료로 사용하였다.Radish used in this experiment was used as a sample while refrigerated after washing domestic radish purchased in Cheongju, Chungcheongbuk-do.
(2) 무청 추출액 A 제조(2) Preparation of rust-free extract A
무청 추출액의 제조는 도 1에 나타낸 바와 같다. 도 1은 무청 추출액의 제조공정을 도식화한 것이다.The preparation of the rust free extract is as shown in FIG. 1 . 1 is a schematic diagram of the manufacturing process of a rust free extract.
무청 추출액 제조를 위해 냉동 저장한 무청을 해동하여 탈수한 후 55℃로 조절한 건조기(LD-918TH, L'EQUIP, Korea)에서 12시간 동안 열풍 건조하였다. 이후 건조된 시료를 분쇄기(FM-681, Hanil, Haman, Korea)로 분쇄하고, 분쇄된 분말의 20배에 해당하는 증류수를 가하여 100℃에서 6시간 동안 열수 추출하였다. 열수 추출한 무청 추출액을 감압여과한 후 농축하여 무청 추출액 A를 제조하였다.After thawing and dehydrating frozen radish to prepare a radish extract, it was dried with hot air for 12 hours in a dryer (LD-918TH, L'EQUIP, Korea) controlled at 55°C. Thereafter, the dried sample was pulverized with a pulverizer (FM-681, Hanil, Haman, Korea), distilled water equivalent to 20 times the pulverized powder was added, and hot water extraction was performed at 100° C. for 6 hours. After filtration under reduced pressure of the extract with hot water, the extract was concentrated to prepare the extract with no rust A.
도 2는 무청 추출액 제조공정을 보여주는 각 단계별 사진이다.Figure 2 is a photograph showing each step of the manufacturing process of the extract without rust.
(3) 무청 추출액 B 제조(3) Preparation of extract B without rust
효과적인 무청 식이섬유 추출을 위해 냉동 저장한 무청을 해동, 탈수, 건조, 분쇄한 후 열수 추출 시 분쇄된 무청 분말의 3 중량% 양의 구연산을 첨가하는 것을 제외하고는 무청 추출액 A 제조와 동일한 방식으로 무청 추출액 B를 제조하였다. For effective extraction of rust-free dietary fiber, thaw, dehydrate, dry, and pulverize frozen radish, and then use the same method as in the preparation of extract A without adding citric acid in an amount of 3% by weight of the pulverized radish powder during hot water extraction. A rust-free extract B was prepared.
도 3은 본 발명에 따라 제조된 무청 추출액 A 및 무청 추출액 B의 사진이다.Figure 3 is a photograph of the non-corrosive extract A and the non-corrosive extract B prepared according to the present invention.
(4) 무청 추출액의 적정 농도 설정(4) Setting the appropriate concentration of rust-free extract
무청 추출액의 적정 농도 설정을 위해 농축시간에 따른 °Brix를 측정하였다. 무청 추출액 50 g를 증발접시에 넣고 수욕 중에서 증발 건조한 후, 이를 식힌 다음 칭량하여 증발접시의 전후의 중량차를 측정하였으며 굴절당도계(Hand Refractometer, Atago)를 이용하여 °Brix를 측정하였다. °Brix was measured according to the concentration time to set the appropriate concentration of the rust-free extract. After putting 50 g of the rust-free extract into an evaporation dish, it was evaporated to dryness in a water bath, cooled and weighed to measure the weight difference before and after the evaporation dish, and °Brix was measured using a hand refractometer (Atago).
무청 추출액 적정농도 설정을 위해 농축시간에 따른 °Brix와 증발 접시의 전후 중량차를 측정하였다. 농축 2시간 후 무청 추출액은 2°Brix에서 15°Brix로 변화하였으며 전후 중량은 하기 표 1에 나타낸 바와 같다.To set the optimum concentration of the rust free extract, the difference in weight between °Brix and the evaporation dish according to the concentration time was measured. After 2 hours of concentration, the rust-free extract was changed from 2°Brix to 15°Brix, and the before and after weights are as shown in Table 1 below.
이하 15°Brix의 무청 추출액을 추가 농축하여 34°Brix의 무청 추출액이 제조하였다. 전후 중량은 다음 표 2와 같다.Hereinafter, a rustless extract of 15°Brix was further concentrated to prepare a rustless extract of 34°Brix. The weight before and after is shown in Table 2 below.
(5) 무청 추출액의 식이섬유 함량 분석(5) Analysis of the dietary fiber content of the radish extract
무청 추출액 A 및 B의 식이섬유 함량은 식품공전 상의 식이섬유 추출 분석법 중 효소 중량법을 사용, 주관기관의 식이섬유 추출장치를 활용해 분석하였다. 건조된 무청 추출액 시료 두 개를 준비하고 이를 내열성 α-아밀라아제(Thermophile α-amylase), 프로테아제, 아밀로글루코시다제 효소로 연속적으로 분해하여 전분과 단백질을 제거하였다. 총 식이섬유(TDF) 정량은 효소분해물에 녹아 있는 식이섬유를 에탄올로 처리하여 침전시켜 여과하고 에탄올과 아세톤으로 세척한 후, 건조하여 그 무게를 확인하였다. 불용성 식이섬유(IDF)는 효소분해물을 여과하여 잔사(residue)를 따뜻한 물로 세척하고 그 무게를 확인하여 정량하였다. 수용성식이섬유(SDF)는 IDF의 전처리과정에서 얻은 여과액과 세척액을 합하여 에탄올로 침전시킨 후 여과하여 잔류물을 건조하고 무게를 확인하여 정량하였다. 그 결과는 하기 표 3에 나타내었다.The dietary fiber content of the radish extracts A and B was analyzed using the enzyme weight method among the dietary fiber extraction analysis methods in the Food Standards Code, and using the dietary fiber extraction device of the competent institution. Two dried radish extract samples were prepared, and they were successively digested with heat-resistant α-amylase, protease, and amyloglucosidase enzymes to remove starch and proteins. To determine the total dietary fiber (TDF), the dietary fiber dissolved in the enzymatic digest was treated with ethanol, precipitated, filtered, washed with ethanol and acetone, and dried to determine the weight. Insoluble dietary fiber (IDF) was quantified by filtering the enzymatic digest, washing the residue with warm water, and checking the weight. Soluble dietary fiber (SDF) was quantified by combining the filtrate and washing solution obtained in the pretreatment of IDF, precipitating it with ethanol, filtration, drying the residue, and checking the weight. The results are shown in Table 3 below.
그 결과, 상기 표 3에서 보듯이, 무청 추출액 A의 식이섬유 함량은 76.4 mg/g 으로 나타났으며, 무청 추출액 B의 식이섬유 함량은 102.9 mg/g로 나타났다. 구연산을 첨가해 열수 추출한 무청 추출액 B의 식이섬유 함량이 높은 것을 보아, 구연산 첨가를 통해 식이섬유 추출이 더욱 효과적으로 이루어졌음을 확인할 수 있었다.As a result, as shown in Table 3, the dietary fiber content of the extract A was 76.4 mg/g, and the fiber content of the extract B was 102.9 mg/g. Seeing that the dietary fiber content of radish extract B extracted with hot water with the addition of citric acid was high, it was confirmed that the dietary fiber extraction was more effective through the addition of citric acid.
(6) 무청 추출액의 일반성분 분석(6) Analysis of general components of extracts without rust
무청 추출액의 일반성분 분석은 식품공전 및 AOAC의 방법을 참고하여 실시하였다. 수분함량은 105℃ 상압가열 건조법(J-DSA2, Jisico Co., Seoul, Korea), 회분은 550℃의 직접 회화법(J-FM, Jeil science Co., Gyeonggi, Korea)을 이용하여 분석하였다. 지방의 함량은 주관기관의 조지방 분석장치를 활용, 산 분해법(Foss tecator digestor auto & Kjeltec auto 2300, Foss, MA, USA)으로 측정하고 단백질의 함량 역시 주관기관이 보유한 조단백 분석장치를 활용 micro-Kjeldahl의 질소 정량법(Foss Kjeltec 820, Foss, MA, USA)(Foss tecator digestor auto & Kjeltec auto 2300, Foss, MA, USA)을 사용하였다. 탄수화물 함량은 100에서 수분, 조회분, 조단백질, 조지방의 중량을 뺀 값으로 계산하였다. 그 결과는 하기 표 4에 나타내었다.The analysis of general components of the extract was carried out with reference to the Food Standards Code and the AOAC method. The moisture content was analyzed using the atmospheric heating and drying method at 105°C (J-DSA2, Jisico Co., Seoul, Korea) and the ash content was analyzed using the direct ashing method at 550°C (J-FM, Jeil science Co., Gyeonggi, Korea). The fat content is measured by the acid digestion method (Foss tecator digestor auto & Kjeltec auto 2300, Foss, MA, USA) using the crude fat analyzer of the host institution, and the protein content also uses the crude protein analyzer owned by the host institution micro-Kjeldahl Nitrogen quantification method (Foss Kjeltec 820, Foss, MA, USA) (Foss tecator digestor auto & Kjeltec auto 2300, Foss, MA, USA) was used. Carbohydrate content was calculated by subtracting the weight of water, crude flour, crude protein, and crude fat from 100. The results are shown in Table 4 below.
무청 추출액의 일반성분 분석을 각 5회씩 실시하였다. 분석 결과 상기 표 4에서 보듯이 무청 추출액 A는 탄수화물 18.01%, 수분 69.78%, 회분 4.81%, 단백질 6.93%, 지방 0.47%로 나타났으며, 무청 추출액 B는 탄수화물 17.63%, 수분 71.905, 회분 4.77%, 단백질 5.46%, 지방 0.24%로 나타났다.The general component analysis of the radish extract was carried out 5 times each. As a result of the analysis, as shown in Table 4 above, the unsweetened extract A contained 18.01% carbohydrate, 69.78% water, 4.81% ash, 6.93% protein, and 0.47% fat. , protein 5.46% and fat 0.24%.
(7) 무청 추출액의 Vitamin C 및 Fe 함량(7) Vitamin C and Fe content of the radish extract
Vitamin C는 무청 추출액 1 g을 10% metaphosphate 용액 50 mL를 첨가하고 10분간 shaking한 후 5% metaphosphate 용액으로 100 mL 정용시키고, 3,000 rpm에서 10분간 원심분리 후 상등액을 취해 0.2 μm membrane filter를 통과시켜 여과 후 HPLC로 분석하였다. For Vitamin C, 50 mL of 10% metaphosphate solution is added to 1 g of the rust-free extract, and after shaking for 10 minutes, 100 mL of 5% metaphosphate solution is used. After centrifugation at 3,000 rpm for 10 minutes, the supernatant is taken and passed through a 0.2 μm membrane filter. After filtration, it was analyzed by HPLC.
Fe 함량은 무청 추출액 1 g에 미리 제조한 HCl (증류수:HCl=1:1) 20 mL를 가하여 가열시킨 후 잔여물을 증류수 100 mL에 희석시키고, 이를 1시간 이상 가열한 다음 방냉시켜 100 mL로 정용 및 여과 후 spectrophotometer로 측정하였다.The Fe content is determined by adding 20 mL of previously prepared HCl (distilled water: HCl=1:1) to 1 g of the rust-free extract, heating it, and diluting the residue in 100 mL of distilled water, heating it for at least 1 hour, and then cooling it to 100 mL. After dialysis and filtration, it was measured with a spectrophotometer.
무청 추출액의 Vitamin C와 Fe를 분석한 결과는 하기 표 5에 나타내었다.The results of analyzing the vitamin C and Fe of the radish extract are shown in Table 5 below.
상기 표 5에서 보듯이, 무청 추출액 A, 무청 추출액 B 모두 Vitamin C는 불검출되었으며, 칼슘은 무청 추출액 A는 8,422.0 mg/100g, B는 8,816.0 mg/100g, 철은 각각 4.48, 4.84 mg/100g 으로 나타났다. As shown in Table 5 above, vitamin C was not detected in both the extract A and the extract B, and the calcium was found to be 8,422.0 mg/100g for the unrusted extract A, 8,816.0 mg/100g for B, and 4.48 and 4.84 mg/100g for iron, respectively. .
(8) 무청 추출액의 생리활성 분석(8) Analysis of the physiological activity of the extract
① 총 폴리페놀 함량① Total polyphenol content
총 폴리페놀 함량은 Folin-Denis 방법으로 측정하였다. 제조된 무청 추출액을 1.0 mg/mL의 농도로 3차 증류수에 희석한 액 0.1 mL에 2 N Folin reagent(Sigma, USA) 0.5 mL, 증류수 8.4 mL를 가하고 3분간 정치한 다음 1.0 mL의 20% Na2CO3 용액을 가하였다. 이 혼합액을 1시간 동안 정치한 후 분광광도계를 사용하여 725 nm에서 흡광도를 측정하고 gallic acid (Sigma, USA)를 이용하여 작성한 표준곡선으로부터 총 폴리페놀 함량을 mg/g로 구하였다. 무청 추출액의 총 폴리페놀 함량을 측정한 결과는 하기 표 6과 같다. Total polyphenol content was determined by Folin-Denis method. To 0.1 mL of the prepared rust-free extract diluted in tertiary distilled water to a concentration of 1.0 mg/mL, 0.5 mL of 2 N Folin reagent (Sigma, USA) and 8.4 mL of distilled water are added, left for 3 minutes, and then 1.0 mL of 20% Na 2 CO 3 solution was added. After standing this mixture for 1 hour, absorbance was measured at 725 nm using a spectrophotometer, and the total polyphenol content in mg/g was obtained from a standard curve prepared using gallic acid (Sigma, USA). The results of measuring the total polyphenol content of the radish extract are shown in Table 6 below.
상기 표 6에서 보듯이, 무청 추출액 A는 1980.78 mg/100g으로 나타났으며, 무청 추출액 B는 2073.06 mg/100g 으로 나타나, 무청 추출액 B가 더 높은 폴리페놀 함량을 나타내었다. As shown in Table 6, the amount of the extract A without rust was found to be 1980.78 mg/100 g, and the amount of the extract without the extract B was found to be 2073.06 mg/100 g.
② DPPH 전자공여능② DPPH electron donating ability
전자공여능은 시료의 flavonoids 및 폴리페놀 물질 등에 대한 항산화 작용의 지표로 free radical을 환원시키거나 상쇄시키는 능력이 커서 높은 항산화 활성 및 활성산소에 대한 소거활성을 기대할 수 있다고 보고되어 있다. 전자공여능은 추출액에 대한 DPPH(α,α-diphenyl-2-picryl-hydrazyl, Sigma, USA)의 전자공여효과로 각 시료의 환원력을 측정하였다. 무청 추출액을 1.0 mg/mL의 농도로 3 차 증류수에 희석한 액 0.2 mL에 4x10-4 M DPPH용액(99% 에탄올 에 용해) 0.8 mL, 99% 에탄올 2 mL를 가하여 총액의 부피가 3 mL가 되도록 하였다. 이 반응액을 약 10 초간 혼합하여 실온에 15분 방치한 후 분광광도계(V-550 spectrophotometer, Jasco, Japan)를 사용하여 525 nm에서 흡광도를 측정하였다. 무청 추출액의 전자공여능은 하기 수학식 1에 따라 산술하였으며, 그 결과는 하기 표 7에 나타내었다. It has been reported that electron donating ability is an index of the antioxidant action on flavonoids and polyphenols of the sample, and has a high ability to reduce or cancel free radicals, so that high antioxidant activity and scavenging activity against free radicals can be expected. The electron donating ability was measured by the electron donating effect of DPPH (α,α-diphenyl-2-picryl-hydrazyl, Sigma, USA) to the extract, and the reducing power of each sample was measured. Add 0.8 mL of 4x10-4 M DPPH solution (dissolved in 99% ethanol) and 2 mL of 99% ethanol to 0.2 mL of a solution diluted in tertiary distilled water at a concentration of 1.0 mg/mL to make the total volume of 3 mL. made to be The reaction solution was mixed for about 10 seconds, left at room temperature for 15 minutes, and then absorbance was measured at 525 nm using a spectrophotometer (V-550 spectrophotometer, Jasco, Japan). The electron donating ability of the radish extract was calculated according to Equation 1 below, and the results are shown in Table 7 below.
A : 추출액 첨가구의 흡광도A: Absorbance of extract solution addition port
B : 추출액 무 첨가구의 흡광도B: Absorbance of extract without addition
상기 표 7에서 보듯이, DPPH 전자공여능은 무청 추출액 A는 38.66%, 무청 추출액 B는 40.58%로 나타났다. As shown in Table 7, the DPPH electron donating ability was 38.66% for the unstained extract A and 40.58% for the unstained extract B.
③ 아질산염 소거작용③ Nitrite scavenging action
아질산염은 amine류와 반응하여 nitrosamine을 생성하는 것으로 알려져 있다. 이것은 체내에서 diazoalkane으로 전환되어 핵산, 단백질 등의 세포내 성분들을 alkyl화하여 암을 유발하는 것으로 알려져 있다. It is known that nitrites react with amines to form nitrosamines. It is converted into diazoalkane in the body and is known to cause cancer by alkylating intracellular components such as nucleic acids and proteins.
아질산염 소거작용은 1 mM NaNO2 0.1 mL에 무청 추출액을 1.0 mg/mL의 농도로 3차 증류수에 희석한 액 0.2 mL와 0.1 N HCl를 0.7 mL 가하여 반응용액의 부피를 1mL로 하였다. 이를 37℃에서 1시간 동안 반응시킨 후 2% acetic acid 5 mL, Griess 시약(Sigma, USA) 0.4 mL를 가하여 잘 혼합 시켜 15분간 실온에서 방치시킨 후 분광광도계를 사용하여 520 nm에서 흡광도를 측정하여 잔존하는 아질산염량을 구하였다. 이때 대조구는 Griess 시약 대신 증류수 0.4 mL을 가하여 상기와 동일하게 행하였다. 무청 추출액의 아질산염 소거능은 하기 수학식 2에 따라 산술하였으며, 그 결과는 하기 표 8에 나타내었다. For the nitrite scavenging action, 0.2 mL of a solution obtained by diluting a rust-free extract in 0.1 mL of 1 mM NaNO2 in tertiary distilled water to a concentration of 1.0 mg/mL and 0.7 mL of 0.1 N HCl was added to make the volume of the reaction solution 1 mL. After reacting this at 37°C for 1 hour, add 5 mL of 2% acetic acid and 0.4 mL of Griess reagent (Sigma, USA), mix well, leave at room temperature for 15 minutes, and measure absorbance at 520 nm using a spectrophotometer. The amount of nitrite remaining was calculated|required. In this case, the control was performed in the same manner as above by adding 0.4 mL of distilled water instead of the Griess reagent. The nitrite scavenging ability of the non-rusk extract was calculated according to Equation 2 below, and the results are shown in Table 8 below.
N : 아질산염 소거능N: nitrite scavenging ability
A : 1 mM NaNO2 용액에 시료를 첨가하여 1시간 반응시킨 후의 흡광도A: Absorbance after adding sample to 1 mM NaNO2 solution and reacting for 1 hour
B : 1 mM NaNO2 용액에 시료 대신 증류수를 첨가하여 1시간 반응시킨 후의 흡광도B: Absorbance after reaction for 1 hour by adding distilled water instead of sample to 1 mM NaNO 2 solution
C : 시료 추출액 자체의 흡광도C: Absorbance of the sample extract itself
상기 표 8에서 보듯이, pH 2.07에서 아질산염 소거작용을 측정하였을 때 무As shown in Table 8 above, when the nitrite scavenging activity was measured at pH 2.07, no
청 추출액 A는 61.17%, 무청 추출액 B는 65.73%를 나타내었다. pH 4.2에서는 무청 추출액 A 42.45%, 무청 추출액 B 42.30%로 낮아지는 것을 확인하였다. 추출 시 시료량과 농도를 높이고, 본 실험에서 사용한 pH 2.07보다 낮은 pH에서는 아질산 소거능이 높아질 것으로 여겨진다.Blue extract A showed 61.17%, and non-blue extract B showed 65.73%. At pH 4.2, it was confirmed that the rust-free extract A 42.45% and the non-corrosive extract B 42.30% were lowered. It is believed that the nitrite scavenging ability will increase at a pH lower than the pH 2.07 used in this experiment, increasing the sample amount and concentration during extraction.
④ SOD 유사활성④ SOD-like activity
SOD 유사활성 물질은 효소는 아니지만 SOD와 유사한 역할을 하는 저분자 물질로 주로 phytochemicals에 속하며 superoxide의 반응성을 억제하여 산화적 장애를 방어할 수 있다고 알려져 있다. SOD 유사활성 측정은 추출액을 감압 농축한 후 Tris-HCl buffer(50 mM tris[hydroxymethyl] amino-methaneㅁ10 mM EDTA, pH 8.5)를 이용하여 pH 8.5로 조절된 시료액을 만들었다. 1.0 mg/mL 농도의 시료 0.2 mL에 tris-HCl buffer(pH 8.5) 3 mL와 7.2 mM pyrogallol 0.2 mL를 가하고 25℃에서 10분간 방치한 후 1 N HCl 1 mL로 반응을 정지시킨 후 분광광도계를 이용하여 420 nm에서 흡광도를 측정하여 차이를 백분율로 나타냈다. 무청 추출액의 SOD 유사활성은 하기 수학식 3에 따라 산술하였으며, 그 결과는 하기 표 9에 나타내었다. Although SOD-like active substances are not enzymes, they are low-molecular substances that play a similar role to SOD, mainly belonging to phytochemicals, and are known to be able to defend against oxidative disorders by inhibiting the reactivity of superoxide. For measurement of SOD-like activity, a sample solution adjusted to pH 8.5 was prepared using Tris-HCl buffer (50 mM tris[hydroxymethyl] amino-methane ㅁ10 mM EDTA, pH 8.5) after concentrating the extract under reduced pressure. 3 mL of tris-HCl buffer (pH 8.5) and 0.2 mL of 7.2 mM pyrrogallol were added to 0.2 mL of a sample with a concentration of 1.0 mg/mL, left at 25°C for 10 minutes, and the reaction was stopped with 1 mL of 1 N HCl, followed by a spectrophotometer. Absorbance was measured at 420 nm using the The SOD-like activity of the radish extract was calculated according to Equation 3 below, and the results are shown in Table 9 below.
A : 추출액 첨가구의 흡광도A: Absorbance of extract solution addition port
B : 추출액 무 첨가구의 흡광도B: Absorbance of extract without addition
단, A, B는 대조구의 흡광도를 제외한 수치임However, A and B are values excluding the absorbance of the control.
상기 표 9에서 보듯이, SOD 유사 활성은 무청 추출액 A, B가 각각 32.61%, 32.88%로 나타나, 전체적으로 무청 추출액의 항산화 활성은 30%를 넘는 것으로 나타났다. As shown in Table 9, the SOD-like activity of the radish extracts A and B were 32.61% and 32.88%, respectively, indicating that the antioxidant activity of the radish extract as a whole exceeded 30%.
⑤ MTT 방법을 이용한 무청 추출액의 독성검사⑤ Toxicity test of extracts of green radish using MTT method
3T3-L1 전지방세포주를 96well plate에 분주하여 24시간 배양한 후, 추출액을 농도별 (0, 10, 25, 50, 100, 150, 200μg/mL)로 처리하여 24시간 배양하였다. 3-(4,5-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) 시약을 10 μL 처리하여 4시간 배양한 후, 배지와 MTT 시약을 제거하고, DMSO 시약 100 μL를 가하여 570 nm에서 ELISA reader를 이용하여 세포독성을 측정하였다.The 3T3-L1 whole adipocyte line was dispensed into a 96-well plate and cultured for 24 hours, and the extract was treated with each concentration (0, 10, 25, 50, 100, 150, 200 μg/mL) and cultured for 24 hours. Treated with 3-(4,5-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent with 10 μL and incubated for 4 hours, remove the medium and MTT reagent, and add 100 μL of DMSO reagent to 570 Cytotoxicity was measured at nm using an ELISA reader.
그 결과, 3T3-L1 전지방 세포 분화과정 중 무청 추출액을 다양한 농도로 처리하여 분화 8일째에 XTT 방법으로 세포독성을 측정하였다. 선행연구를 통해 무청 추출액을 50~300 μg/mL의 농도로 처리한 후 세포생존 정도를 측정한 결과 300μg/mL부터 유의적으로 세포 성장과 분화에 영향을 나타내었다. 따라서 이후의 지방 축적 억제효과 시험에서는 50, 100 및 200 μg/mL의 안전한 분획물 농도에서 연구를 수행하였다. 무청 추출액의 세포독성 결과를 다른 천연물 또는 식품소재의 분획물을 연구한 보고와 비교해볼 때 안전 범위가 넓게 나타나고 있어 세포독성이 상대적으로 낮은 것으로 여겨진다.As a result, cytotoxicity was measured by the XTT method on the 8th day of differentiation by treating the radish extract at various concentrations during the 3T3-L1 pre-adipocyte differentiation process. As a result of measuring the degree of cell viability after treating the radish extract at a concentration of 50 to 300 μg/mL through a previous study, it showed a significant effect on cell growth and differentiation from 300 μg/mL. Therefore, in the subsequent fat accumulation inhibitory effect test, studies were conducted at safe fraction concentrations of 50, 100 and 200 μg/mL. When comparing the cytotoxicity results of the radish extract with the reports of studies on fractions of other natural products or food materials, the safety range is wide and the cytotoxicity is considered to be relatively low.
⑥ Oil Red O 염색법을 이용한 무청 추출액의 지방축적 억제효과⑥ Fat accumulation inhibitory effect of radish extract using Oil Red O dyeing method
전지방세포인 3T3-L1 세포주를 American Type Culture Collection(ATCC)에서 구입하여 10% fetal bovine serum(FBS), 1% penicillin/streptomycin이 첨가된 Dulbecco's modified Eagle's medium(DMEM)배지에 2x105 cells/mL 농도로 부유시켜 48시간 동안 배양하여 confluent 상태가 되게 37℃ 항온기에서 배양하였다. 분화배지(10% FBS, 1% penicillin/streptomycin, 5 μg/mL insulin, 1 μM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine)로 교체하여 2일간 배양 후, 지방세포로의 분화를 촉진하기 위해 5 μg/mL insulin이 포함된 DMEM 배지로 3일간 배양하였다. 무청 추출액 처리군은 각각 25, 50, 100 μg/mL의 농도로 처리하여 분화 시에 같이 처리하였다. 그리고 중성지질이 존재하는 지방세포만을 염색할 수 있는 Oil Red O 염색액을 이용하여 염색한 후, isopropanol로 염색액을 추출하여 510 nm 에서 ELISA reader를 이용하여 지방세포내의 중성지질의 정도를 측정하였다.Whole adipocytes, 3T3-L1 cell line, were purchased from the American Type Culture Collection (ATCC), and 2x10 5 cells/mL in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. It was suspended at a concentration and incubated for 48 hours in a 37°C incubator to become confluent. After replacing with differentiation medium (10% FBS, 1% penicillin/streptomycin, 5 μg/mL insulin, 1 μM dexamethasone, 0.5 mM 3-isobutyl-1-methylxanthine) and culturing for 2 days, 5 to promote differentiation into adipocytes It was cultured for 3 days in DMEM medium containing μg/mL insulin. The unsweetened extract treated group was treated at a concentration of 25, 50, and 100 μg/mL, respectively, and treated at the time of differentiation. And after staining with Oil Red O staining solution that can stain only adipocytes with neutral lipids, the staining solution was extracted with isopropanol and the level of neutral lipids in adipocytes was measured using an ELISA reader at 510 nm.
무청 추출액 처리에 의한 3T3-L1 세포의 분화과정 중 지방구의 생성 억제 유무를 Oil Red O 염색법을 이용하여 측정하였다. Oil Red O 염색시약은 중성지질, 콜레스테롤 등과 결합하며, 지방분화로 인해 축적된 대부분의 지질은 중성지방으로 염색된 세포의 붉은색 정도를 통해 분화 정도를 확인할 수 있다. 완전히 분화된 지방세포(100%)와 비교하였을 때, 무청 추출액을 50 및 100 μg/mL를 처리한 군에서는 지방 축적이 유의적으로 감소하지 않았으나, 200 μg/mL의 농도를 처리한 군에서는 지방 축적이 10.8% 유의적으로 감소함으로써 지방구 생성 억제효과가 있음을 확인하였다.The presence or absence of inhibition of the production of adipocytes during the differentiation process of 3T3-L1 cells by treatment with radish extract was measured using Oil Red O staining method. Oil Red O staining reagent binds with triglycerides and cholesterol, and the degree of differentiation of most lipids accumulated due to fat differentiation can be confirmed by the degree of redness of cells stained with triglycerides. Compared with fully differentiated adipocytes (100%), fat accumulation was not significantly reduced in the groups treated with 50 and 100 µg/mL of the radish extract, but fat accumulation was not significantly reduced in the group treated with 200 µg/mL of the extract. As the accumulation significantly decreased by 10.8%, it was confirmed that there was an inhibitory effect on the formation of fat cells.
<실시예 2> 무청 추출액을 함유하는 노인식 고단백 경구용 조성물 제조<Example 2> Preparation of a composition for oral use with high protein for the elderly containing a green radish extract
(1) 재료(1) Material
무청 추출액을 함유하는 노인식 고단백 경구용 조성물에 사용된 무청 추출액 B는 충북 청주지역에서 구매한 국내산 무청을 세척, 건조, 분쇄, 구연산 첨가 및 열수추출, 감압여과, 농축한 후 냉장 보관하면서 시료로 사용하였다. The radish extract B used in the elderly-style high-protein oral composition containing radish extract is used as a sample while refrigerated after washing, drying, grinding, citric acid addition, hot water extraction, vacuum filtration, and concentration of domestic radish purchased in Cheongju, Chungbuk. did.
(2) 무청 추출액을 함유하는 노인식 고단백 경구용 조성물 제조(2) Preparation of a composition for oral use with high protein for the elderly containing a green radish extract
무청 추출액 함유 노인식 고단백 경구용 조성물은 구연산을 첨가해 추출한 무청 추출액 B와 익산지역의 쌀을 활용한 쌀 추출액과 대체감미료인 알룰로오스 및 프락토 올리고당을 첨가하고, 여기에 난소화성 말토덱스트린, 분리대두단백, 황산마그네슘, 글리콘산 아연, 정제 소금, 비타민 혼합제제, 정제수를 혼합 및 가열하여 제조하였다.The elderly food high-protein oral composition containing radish extract contains citric acid extract B, rice extract using rice from Iksan region, and alternative sweeteners allulose and fructooligosaccharide, and indigestible maltodextrin, separated It was prepared by mixing and heating soy protein, magnesium sulfate, zinc glycolate, refined salt, a vitamin mixture, and purified water.
영양불량에 노출되기 쉽고 소화 기능이 저하된 노인을 위해 단백질과 식이섬유가 높게 함유된 경구식을 제조하였다. 분리대두단백 첨가를 통해 단백질 함량을 증가시켰으며, 식이섬유 함량 증가를 위해 프락토 올리고당, 난소화성 말토덱스트린을 첨가하였다. 또한 익산지역의 쌀을 활용한 쌀 추출물을 제조하여 경구식에 첨가하였으며, 스파우트 파우치 형식의 튜브형 포장지를 사용, 기존 250 mL였던 경구식 용량을 200 mL로 변경하여 무청 추출액 함유 노인식 고단백 경구용 조성물을 제조하였다.An oral meal containing high protein and dietary fiber was prepared for the elderly who are easily exposed to malnutrition and have reduced digestive function. Protein content was increased by adding soy protein isolate, and fructooligosaccharide and indigestible maltodextrin were added to increase dietary fiber content. In addition, a rice extract using rice from the Iksan region was prepared and added to the oral diet. Using spout pouch-type tubular packaging, the oral dose from 250 mL was changed to 200 mL, and an elderly-style high-protein oral composition containing unsweetened extract was prepared. prepared.
최종 무청 추출액을 함유하는 노인식 고단백 경구용 조성물 제품의 배합비는 다음과 표 10과 같으며, 비타민 혼합제제의 비율은 하기 표 11과 같다. 도 4는 본 발명에 따른 무청 추출액 함유 노인식 고단백 경구용 조성물의 제조공정을 도식화한 것이다.The compounding ratio of the elderly-type high-protein oral composition product containing the final radish extract is shown in Table 10 below, and the ratio of the vitamin mixture is shown in Table 11 below. Figure 4 is a schematic diagram of the manufacturing process of the composition for oral use of the elderly food containing a radish extract according to the present invention.
도 5는 본 발명에 따라 제조된 무청 추출액 함유 노인식 고단백 경구용 조성물이 포장된 상품의 일 례를 보여주는 것이다.5 shows an example of a packaged product in which the elderly-style high-protein oral composition containing a radish extract prepared according to the present invention is packaged.
<시험예 1> 무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 품질평가<Test Example 1> Quality evaluation of elderly-type high-protein oral composition containing radish extract
① 일반성분 분석① General component analysis
무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 일반성분 분석을 실시하였다. 일반성분 분석은 식품공전 및 AOAC의 방법을 참고하여 실시하였다. 수분함량은 105℃ 상압가열 건조법(J-DSA2, Jisico Co., Seoul, Korea), 회분은 550℃의 직접 회화법 (J-FM, Jeil science Co., Gyeonggi, Korea)을 이용하여 분석하였다. 지방의 함량은 주관 기관의 조지방 분석장치를 활용, 산 분해법(Foss tecator digestor auto & Kjeltec auto 2300, Foss, MA, USA)으로 측정하고 단백질의 함량 역시 주관기관이 보유한 조단백 분석장치를 활용 micro-Kjeldahl의 질소 정량법(Foss Kjeltec 820, Foss, MA, USA)(Foss tecator digestor auto & Kjeltec auto 2300, Foss, MA, USA)을 사용하였다. 탄수화물 함량은 100에서 수분, 조회분, 조단백질, 조지방의 중량을 뺀 값으로 계산하였다. 무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 일반성분 분석 결과는 표 12에 나타내었다.containing unsweetened extract General component analysis of the composition for oral high-protein elderly diet was performed. General component analysis was carried out by referring to the method of the Food Standards Code and AOAC. Moisture content was analyzed using atmospheric heating and drying at 105°C (J-DSA2, Jisico Co., Seoul, Korea), and ash content at 550°C by direct aeration (J-FM, Jeil science Co., Gyeonggi, Korea). The fat content was measured by the acid digestion method (Foss tecator digestor auto & Kjeltec auto 2300, Foss, MA, USA) using the crude fat analyzer of the host institution, and the protein content was also measured using the crude protein analyzer owned by the host institution micro-Kjeldahl Nitrogen quantification method (Foss Kjeltec 820, Foss, MA, USA) (Foss tecator digestor auto & Kjeltec auto 2300, Foss, MA, USA) was used. Carbohydrate content was calculated by subtracting the weight of water, crude flour, crude protein, and crude fat from 100. containing unsweetened extract Table 12 shows the results of analysis of the general components of the elderly high-protein oral composition.
열량, 단백질, 탄수화물, 당류, 지방, 포화지방, 트랜스지방, 콜레스테롤, 나트륨 분석을 실시하였다. 분석 결과 열량 65.47 kcal/100g, 단백질 5.36 g/100g, 탄수화물 10.76 g/100g, 당류 5.31 g/100g, 지방 0.11 g/100g, 포화지방 0.04 g/100g, 트랜스지방 0.00 g/100g, 콜레스테롤 불검출, 나트륨 211.83 mg/100g 으로 나타났다.Calorie, protein, carbohydrate, saccharide, fat, saturated fat, trans fat, cholesterol, and sodium were analyzed. Analysis Result Calorie 65.47 kcal/100g, protein 5.36 g/100g, carbohydrate 10.76 g/100g, sugar 5.31 g/100g, fat 0.11 g/100g, saturated fat 0.04 g/100g, trans fat 0.00 g/100g, cholesterol free, sodium It was found to be 211.83 mg/100g.
② 안전성 평가 ② Safety evaluation
무청 추출액 함유 노인식 고단백 경구용 조성물의 미생물 안전성을 평가하기 위해 식품공전상 명시된 방법에 따라 대장균군, 살모넬라 검출시험을 실시하였다.In order to evaluate the microbial safety of the composition for oral high-protein oral composition containing green radish extract, E. coli group and Salmonella detection test were conducted according to the method specified in the Food Standards Code.
무청 추출액 함유 노인식 고단백 경구용 조성물의 미생물 안전성을 평가하기 위해 대장균군, 살모넬라 검출 시험을 실시하였다. 결과적으로 모두 불검출인 것으로 나타나 무청 추출액 함유 노인식 고단백 경구용 조성물의 안전성을 확인하였다.In order to evaluate the microbial safety of the composition for oral administration of high-protein diet for the elderly containing green radish extract, a test for detecting E. coli group and Salmonella was conducted. As a result, it was found that all of them were non-detectable, confirming the safety of the composition for oral use of the elderly food containing the extract of green radish.
<시험예 2> 무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 무기질 함량 측정<Test Example 2> Determination of the mineral content of a composition for oral use with high protein for the elderly containing a radish extract
무기질 분석을 위해 시료 5 g을 550 ℃에서 건식회화, 방냉한 후 증류수로 적시고 HCl:H2O(1:1)용액 10 mL를 가하여 용해시킨 다음 Water bath(SH-GWB11, Samheung Instrument, Seoul, Korea)상에서 증발건조하고 HCl:H2O(1:3)용액 10 mL를 가하여 여과한 후 증류수 100 mL로 정용하여 분석하였다. 전처리 과정을 거친 시험용액의 무기질 함량은 ICP Atomic Emission Spectrometer(ICP-IRIS, Thermo Elemental, Franklin, MA, USA)로 분석하였다. 비교를 위해 무청 추출액을 함유하지 않은 노인식 경구용 조성물을 대조군으로 사용하였다.For mineral analysis, 5 g of the sample was dried at 550 °C, allowed to cool, wetted with distilled water, dissolved by adding 10 mL of HCl:H2O (1:1) solution, and then dissolved in a water bath (SH-GWB11, Samheung Instrument, Seoul, Korea) After evaporation to dryness, 10 mL of a HCl:H 2 O (1:3) solution was added and filtered, followed by analysis with 100 mL of distilled water. The mineral content of the test solution subjected to the pretreatment process was analyzed with an ICP Atomic Emission Spectrometer (ICP-IRIS, Thermo Elemental, Franklin, MA, USA). For comparison, an oral composition for the elderly that does not contain a radish extract was used as a control.
상기 표 13에 나타낸 바와 같이, 본 발명의 무청 추출액 함유 노인식 고단백 경구용 조성물은 대조군에 비하여 무기질 함량이 높은 것을 확인하였다. 특히, Ca과 Fe 함량이 2배 이상 높으므로 노인들에게 좋은 역할을 할 것으로 기대된다.As shown in Table 13 above, it was confirmed that the composition for oral high-protein oral use for the elderly containing radish extract of the present invention had a higher mineral content than the control. In particular, the Ca and Fe content is more than twice as high, so it is expected to play a good role in the elderly.
<시험예 3> 무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 유리아미노산 함량 측정<Test Example 3> Measurement of free amino acid content of elderly-type high-protein oral composition containing radish extract
유리아미노산 분석을 위해 시료 1.0 g씩 취하여 80% 에탄올 10 mL를 가한 다음 20분간 초음파 처리한 후 1,800 g로 4℃에서 10분간 원심 분리하였다. 상기 과정을 2회 반복하고 상등액만을 모아 여과지로 여과한 후, 유리아미노산용 sample dilution buffer(pH 2.2) 1.0 mL에 용해시킨 후 0.45 μm NYLON syringe filter로 2회 여과하여 분석용 시료로 사용하였다. 전처리 과정을 거친 시험용액의 유리아미노산 함량은 아미노산 자동분석기(L-8900 Hitachi High-Technologies Corporation, Tokyo, Japan)를 사용하였으며, S7130 auto sampler와 S2100 solvent delivery system로 분석하였다. 비교를 위해 무청 추출액을 함유하지 않은 노인식 경구용 조성물을 대조군으로 사용하였다.For free amino acid analysis, 1.0 g of each sample was taken, 10 mL of 80% ethanol was added, and then sonicated for 20 minutes, followed by centrifugation at 1,800 g at 4°C for 10 minutes. The above process was repeated twice, and only the supernatant was collected and filtered with filter paper, dissolved in 1.0 mL of sample dilution buffer for free amino acids (pH 2.2), filtered twice with a 0.45 μm NYLON syringe filter, and used as a sample for analysis. The free amino acid content of the test solution subjected to the pretreatment process was analyzed using an automatic amino acid analyzer (L-8900 Hitachi High-Technologies Corporation, Tokyo, Japan), and an S7130 auto sampler and S2100 solvent delivery system. For comparison, an oral composition for the elderly that does not contain a radish extract was used as a control.
상기 표 14에 나타낸 바와 같이, 본 발명의 무청 추출액 함유 노인식 고단백 경구용 조성물은 대조군에 비하여 유리아미노산의 함량이 높은 것을 확인하였다.As shown in Table 14, it was confirmed that the high-protein oral composition for the elderly containing radish extract of the present invention had a higher free amino acid content than the control.
노인이 되면 근육이 감소되어 골다공증, 낙상 등으로 인한 높은 유병율 및 사망률을 보인다. 근육이 부족한 상태에서 질병에 걸리면 회복이 어렵고, 치료를 해도 회복이 더뎌 쉽게 합병증에 빠지고 사망으로 이어지는 경우가 많다. 루이신은 근육 건강에 핵심적 역할을 하는 것으로 알려져 있는 물질로서, 근육 단백질 합성을 증가시키고, 근육 단백질 분해를 감소시켜 근육량 증가에 긍정적인 영향을 끼친다. 이처럼 근육생성에 중요한 루이신은 체내에서 합성되지 않는 필수아미노산으로 반드시 음식으로 섭취해야 하는데 본 발명의 무청 추출액 함유 노인식 고단백 경구용 조성물은 루이신의 함량이 매우 높다.As the elderly age, muscles decrease, resulting in high morbidity and mortality due to osteoporosis and falls. If you fall ill with a lack of muscle, recovery is difficult, and even with treatment, recovery is slow, leading to complications and death in many cases. As a substance known to play a key role in muscle health, leucine has a positive effect on muscle mass by increasing muscle protein synthesis and decreasing muscle protein breakdown. As such, leucine, which is important for muscle growth, is an essential amino acid that is not synthesized in the body and must be ingested with food.
또한, 상기 글루탐산은 동물의 체내에서 신경 전달 물질로 기능을 하며 글루탐산 수용체를 통해 신경 말단의 흥분을 전달한다. 동물과 식물의 단백질로 고루 발견되며 조미료용 염인 모노소듐 글루탐산의 주성분일 뿐만 아니라 엽산의 합성에도 반드시 필요하다. 더욱이 글루탐산을 통해 합성된 엽산이 뇌세포에 축적될 경우 뇌졸중 및 발달 위험성을 감소시키므로 반드시 필요한 성분이다. 본 발명의 무청 추출액 함유 노인식 고단백 경구용 조성물은 글루탐산의 함량이 매우 높은 것으로 확인되었다.In addition, the glutamic acid functions as a neurotransmitter in the body of the animal and transmits the excitation of nerve terminals through the glutamic acid receptor. It is found evenly as a protein in animals and plants, and is not only the main component of monosodium glutamic acid, a salt for seasoning, but also essential for the synthesis of folic acid. Moreover, when folic acid synthesized through glutamic acid is accumulated in brain cells, it is an essential ingredient because it reduces the risk of stroke and development. It was confirmed that the composition for oral administration of high-protein diet for the elderly containing radish extract of the present invention has a very high content of glutamic acid.
<시험예 4> 무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 관능평가<Test Example 4> Sensory evaluation of a composition for oral use with high protein for the elderly containing a radish extract
무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 관능평가는 관능평가 실무교육을 받은 50명을 대상으로 시료의 관능적 품질특징과 평가방법을 교육하고, 그 중 30명을 검사원으로 최종 선발하여 수행하였다. 검사 시 시료는 15℃로 조정하여 임의의 3자리 숫자로 구분된 흰색 종이컵에 담아 제시하였으며, 시료의 색, 풍미, 맛, 전반적인 기호도 등에 대하여 9점 척도법(1=extremely dislike, 9=extremely like)으로 평가하였다.The sensory evaluation of the elderly-style high-protein oral composition containing radish extract was conducted by educating 50 people who received practical training for sensory evaluation on the sensory quality characteristics and evaluation methods of the samples, and 30 of them were finally selected as inspectors. During the test, the sample was adjusted to 15℃ and presented in a white paper cup separated by a random 3-digit number, and a 9-point scale method (1=extremely dislike, 9=extremely like) for the color, flavor, taste, and overall preference of the sample. was evaluated as
무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 관능적 품질 특성을 조사한 결과는 표 15에 나타내었다. containing unsweetened extract The results of examining the sensory quality characteristics of the elderly high-protein oral composition are shown in Table 15.
상기 표 15에서 보듯이, 무청 추출액 함유 노인식 고단백 경구용 조성물은 색 6.36점, 풍미 7.72점, 맛 7.36점으로 평가되었고, 전체적 기호도가 7.19점으로 매우 높은 것으로 나타났다.As shown in Table 15 above, the high-protein oral composition for the elderly containing radish extract was evaluated as color 6.36 points, flavor 7.72 points, and taste 7.36 points, and the overall preference was very high with 7.19 points.
<시험예 5> 무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 유통기한 설정<Test Example 5> Set the shelf life of a composition for oral use with high protein for the elderly containing a green radish extract
무청 추출액을 함유하는 노인식 고단백 경구용 조성물 제품의 유통기한을 설정하기 위하여 식품의약품안전처의 '유통기한 설정기준'고시에 근거하여 기존에 유통 중인 동일한 제품의 유통기한 이내로 설정하였다. 또한 식약처에 고시되어 있는 '유통기한 설정 프로그램'을 활용하여 유통기한 산출 후 여기에 안전계수 0.8을 곱하여 최종 유통기한을 설정하였다.In order to set the shelf life of the high-protein oral composition for the elderly containing the extract, the expiration date was set within the expiration date of the same product in circulation based on the 'Expiration Date Setting Standards' notice of the Ministry of Food and Drug Safety. In addition, the expiration date was calculated using the 'expiry date setting program' announced by the Ministry of Food and Drug Safety, and the final expiration date was set by multiplying this by a safety factor of 0.8.
실온(5℃~35℃)에서 유통되는 무청 추출액 함유 노인식 고단백 경구용 조성물의 유통기한은 15개월로 산출되었다. 여기에 유통과정 중의 안전을 고려하고자 안전 계수 0.8을 곱하여 최종 유통기한을 12개월로 설정하였다.The shelf life of the elderly food high-protein oral composition containing radish extract distributed at room temperature (5℃~35℃) was calculated to be 15 months. In order to consider safety during the distribution process, the final shelf life was set to 12 months by multiplying by a safety factor of 0.8.
<시험예 6> 제형 안정성 시험<Test Example 6> Formulation stability test
무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 제형 안정성 시험을 수행하였다.A formulation stability test of a composition for oral use with high protein for the elderly containing a green radish extract was performed.
관찰 기간은 1주일 동일 실시하였으며, 다양한 온도에서 안정성을 관찰하였고, 그 결과를 하기 표 16에 나타내었다.The observation period was the same for one week, and stability was observed at various temperatures, and the results are shown in Table 16 below.
상기 표 16에서 보듯이, 본 발명에 따라 제조된 무청 추출액을 함유하는 노인식 고단백 경구용 조성물은 다양한 온도에서 안정성이 양호한 것을 확인할 수 있었다.As shown in Table 16 above, it was confirmed that the composition for oral use with high protein for the elderly containing the radish extract prepared according to the present invention had good stability at various temperatures.
이상으로 본 발명의 특정한 부분을 상세히 기술하였는바, 당업계의 통상의 지식을 가진 자에게 있어서 이러한 구체적인 기술은 단지 바람직한 구현예일 뿐이며, 이에 본 발명의 범위가 제한되는 것이 아닌 점은 명백하다. 따라서, 본 발명의 실질적인 범위는 첨부된 청구항과 그의 등가물에 의하여 정의된다고 할 것이다.As described above in detail a specific part of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and it is clear that the scope of the present invention is not limited thereto. Accordingly, the substantial scope of the present invention will be defined by the appended claims and their equivalents.
Claims (4)
무청 추출액을 유효성분으로 포함하고,
상기 무청 추출액은 냉동 저장한 무청을 해동하여 탈수한 후 10 내지 14시간 동안 열풍 건조한 후 분쇄하여 부피비로 15 내지 25배의 증류수를 가하여 90 내지 110℃에서 5 내지 7 시간 동안 열수 추출하여 수득하되, 열수 추출 시 상기 무청 분말의 총 중량을 기준으로 하여 2 내지 5 중량% 양의 구연산을 첨가하여 추출한 것이며,
상기 노인식 고단백 경구용 조성물은 무청 추출액 0.1 내지 10 중량%에 쌀 추출액 5 내지 10 중량%, 대두 단백질 3 내지 8 중량%, 프락토 올리고당 1 내지 3 중량%, 알룰로오스 1 내지 5 중량% 및 비타민 혼합제제 0.02 내지 0.05 중량% 및 잔량의 물을 포함하는 것을 특징으로 하는 노인식 고단백 경구용 조성물.A composition for oral high-protein oral administration for the elderly, comprising:
Containing radish extract as an active ingredient,
The radish extract is thawed and dehydrated, dried with hot air for 10 to 14 hours, then pulverized, added with distilled water 15 to 25 times the volume ratio, and extracted with hot water at 90 to 110° C. for 5 to 7 hours. It is extracted by adding citric acid in an amount of 2 to 5% by weight based on the total weight of the rust-free powder during hot water extraction,
The elderly food high-protein oral composition contains 5 to 10% by weight of rice extract, 3 to 8% by weight of soybean protein, 1 to 3% by weight of fructooligosaccharide, 1 to 5% by weight of allulose, and 1 to 5% by weight of allulose to 0.1 to 10% by weight of the anti-rust extract. A composition for oral use with high protein for the elderly, comprising 0.02 to 0.05% by weight of the mixed formulation and the remaining amount of water.
상기 비타민 혼합제제는 비타민 C와 비타민 B가 97:3 중량비로 혼합된 것을 특징으로 하는 노인식 고단백 경구용 조성물.According to claim 1,
The vitamin mixture formulation is a high-protein oral composition for the elderly, characterized in that vitamin C and vitamin B are mixed in a weight ratio of 97:3.
(S2) 상기 단계 (S1)에서 제조된 무청 추출액 0.1 내지 10 중량%에 쌀 추출액 5 내지 10 중량%, 대두 단백질 3 내지 8 중량%, 프락토 올리고당 1 내지 3 중량%, 알룰로오스 1 내지 5 중량% 및 비타민 혼합제제 0.02 내지 0.05 중량% 및 잔량의 물을 혼합하는 단계;
(S3) 상기 단계 (S2)에서 수득된 혼합물을 90 내지 110℃에서 2 내지 5분 동안 가열하는 단계; 및
(S4) 상기 단계 (S3)에서 가열된 혼합물을 실온에서 25 내지 35 분간 냉각한 다음 4℃에서 냉장시켜 무청 추출액을 함유하는 노인식 고단백 경구용 조성물을 수득하는 단계;를 포함하고,
상기 단계 (S1)에서, 열수 추출 시 상기 무청 분말의 총 중량을 기준으로 하여 2 내지 5 중량% 양의 구연산을 첨가하여 추출하는 것을 특징으로 하는 무청 추출액을 함유하는 노인식 고단백 경구용 조성물의 제조방법.(S1) After thawing and dehydration of frozen radish, dried with hot air for 10 to 14 hours, pulverized, added 15 to 25 times (volume ratio) of distilled water, and hot water extraction at 90 to 110 ° C. for 5 to 7 hours to obtain a rust free extract obtaining;
(S2) 5 to 10% by weight of rice extract, 3 to 8% by weight of soybean protein, 1 to 3% by weight of fructose oligosaccharide, 1 to 5% by weight of allulose in 0.1 to 10% by weight of the unsweetened extract prepared in step (S1) mixing the weight% and 0.02 to 0.05% by weight of the vitamin mixture and the remaining amount of water;
(S3) heating the mixture obtained in step (S2) at 90 to 110° C. for 2 to 5 minutes; and
(S4) cooling the mixture heated in step (S3) at room temperature for 25 to 35 minutes and then refrigeration at 4° C. to obtain a composition for oral use with high protein for the elderly containing a rust-free extract;
In the step (S1), when extracting with hot water, 2 to 5% by weight of citric acid is added based on the total weight of the radish powder. .
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KR20060015997A (en) * | 2004-08-16 | 2006-02-21 | 한국식품연구원 | Preparation method of concentrate of processed radish leaf having high capability of iron absorption, high content of dietary fiber, and inhibition activity of liver cancer |
KR20130082025A (en) * | 2012-01-10 | 2013-07-18 | 씨제이제일제당 (주) | Anabolic protein composition comprising rice bran protein |
KR20150072675A (en) * | 2013-12-20 | 2015-06-30 | 대상 주식회사 | Composition of balanced nutrition for improving frailty and geriatric syndrome and method for preparing the same |
KR20200125155A (en) * | 2019-04-26 | 2020-11-04 | 한국식품연구원 | Composition for improvementing, preventing or treating obesity and metabolic diseases comprising fractions or extract of radish leave |
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KR20060015997A (en) * | 2004-08-16 | 2006-02-21 | 한국식품연구원 | Preparation method of concentrate of processed radish leaf having high capability of iron absorption, high content of dietary fiber, and inhibition activity of liver cancer |
KR20130082025A (en) * | 2012-01-10 | 2013-07-18 | 씨제이제일제당 (주) | Anabolic protein composition comprising rice bran protein |
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