KR102433268B1 - Composition Comprising Exosome for Improving Skin Beauty - Google Patents

Abstract

The present invention relates to a composition for improving skin beauty, and more particularly, relates to a composition for improving skin beauty comprising a human stem cell culture medium (STEMATURE-Exosome^TM) in which exosome production is promoted.

Description

Composition for improving skin beauty containing exosomes {Composition Comprising Exosome for Improving Skin Beauty}

The present invention relates to a composition for improving skin beauty, and more particularly, to a composition for improving skin beauty, including a human stem cell culture medium (STEMATURE-Exosome ^TM ) that promotes exosome production.

Exosome refers to a small vesicle with a membrane structure with a size of 50 - 200 nm that is secreted out of the cell with proteins, DNA, RNA, etc. for signal transduction between cells.

It was observed that these exosomes originate in specific intracellular compartments called multivesicular bodies (MVBs) and are released and secreted out of the cells, rather than directly detaching from the plasma membrane in studies through electron microscopy. That is, when the fusion of the polycystic body and the plasma membrane occurs, such vesicles are released into the extracellular environment, which is called exosomes.

It is known that various types of immune cells, including B-lymphocytes, T-lymphocytes, dendritic cells, megakaryocytes, macrophages, etc., produce and secrete exosomes from stem cells and tumor cells.

Exosomes contain various proteins, DNA, RNA, etc. in the cell. Substances that are contained in these exosomes and secreted out of cells are re-introduced into other cells by fusion or endocytosis with the cell membrane and serve as a communicator between cells. In addition, substances contained in these exosomes and secreted out of the cells can be used to improve skin function and the like.

Under this technical background, the inventors of the present application confirmed that skin beauty can be improved through a human stem cell culture medium (STEMATURE-Exosome ^TM ) in which exosome production is promoted, and completed the present invention.

1. Korea Patent Publication No. 10-2284517 (2021.07.27)

The technical problem to be solved by the present invention is to provide a composition for improving skin beauty containing exosomes.

In order to solve the above-described technical problem, it provides a composition for improving skin beauty, including a human stem cell culture medium (STEMATURE-Exosome ^TM ) in which exosome production is promoted.

The composition is purified water, glycerin, butylene glycol, glycerose, dipropylene glycol, cetyl ethyl hexanoate, triethyl hexanoin, sucrose oleate, oleander leaf extract, allantoin, glyceryl acrylate / acrylic acid Copolymer, polyglyceryl-10 dioleate, pentylene glycol, caprylyl glycol, 1,2-hexanediol and one or more selected from the group consisting of disodium EDTA may be further included have.

Based on the total weight of the composition, exosome production promoted human stem cell culture medium (STEMATURE-Exosome ^TM ) 15-25% (w/w) and purified water 40-50% (w/w) may be included.

The exosome-promoted human stem cell culture medium (STEMATURE-Exosome ^TM ) is treated with a complex composition containing human mesenchymal stem cells, human platelet lysate, serum replacement, sodium pyruvate, insulin, transferrin and sodium selenite. It is a human stem cell culture medium (Human Stem Cell Conditioned Media) obtained by a method for promoting the production of exosomes and/or extracellular vesicles, including the step of culturing in a cultured medium.

The improvement of the skin function may include improvement of skin moisture, improvement of skin tone, improvement of skin whitening by hyperpigmentation, improvement of wrinkles around the eyes, improvement of skin elasticity fatigue, or improvement of skin density.

The composition according to the present invention exhibits the effect of improving skin moisture, improving skin tone, improving skin whitening by hyperpigmentation, improving wrinkles around the eyes, improving skin elasticity, fatigue, or improving skin density, and thus can be usefully used to improve skin function.

The technical effects of the present invention are not limited to those mentioned above, and other technical effects not mentioned will be clearly understood by those skilled in the art from the following description.

1 shows a change in pigmentation of facial skin when a composition according to an embodiment of the present invention is treated.
Figure 2 shows the change in elasticity of the facial skin when the composition according to an embodiment of the present invention is treated.
3 shows a change in the density of the skin around the eyes when the composition according to an embodiment of the present invention is treated.

While the present invention is susceptible to various modifications and variations, specific embodiments thereof are illustrated and shown in the drawings and will be described in detail hereinafter. However, it is not intended to limit the invention to the particular form disclosed, but rather the invention includes all modifications, equivalents and substitutions consistent with the spirit of the invention as defined by the claims.

Although the terms first, second, etc. may be used to describe various elements, components, regions, layers and/or regions, such elements, components, regions, layers and/or regions are not It will be understood that they should not be limited by these terms.

The present invention relates to a composition for improving skin beauty, comprising a human stem cell culture medium (STEMATURE-Exosome ^TM ) in which exosome production is promoted.

The "stem cell" refers to a cell having self-renewal ability (self-renewal) with pluripotent or totipotent capable of differentiating into cells of all tissues of an individual, and the stem cell is an adult Includes stem cells, pluripotent stem cells, induced pluripotent stem cells or embryonic stem cells. The "adult stem cell" refers to a stem cell that appears in the stage of development of each organ of the embryo or at the stage of adulthood as the development process progresses.

"Mesenchymal stem cells" refers to mesenchymal-derived pluripotent progenitor cells before differentiation into cells of specific organs, such as bone, cartilage, fat, tendon, nerve tissue, fibroblasts, and muscle cells. The mesenchymal stem cells are human umbilical cord, Wharton's jelly, umbilical cord blood, placenta, adipose tissue, bone marrow, tonsil, human embryonic yolk sac, umbilical cord, skin, peripheral blood, muscle, liver, It can be derived from nerve tissue, periosteum, fetal membrane, synovial membrane, synovial fluid, amniotic membrane, meniscus, anterior cruciate ligament, articular chondrocytes, baby teeth, perivascular cells, trabecular bone, subpatellar fat mass, spleen, and thymus. Specifically, the mesenchymal stem cells may be adipose, bone marrow, umbilical cord or cord blood-derived stem cells.

The stem cells may be adipose-derived stem cells, and may be isolated from human adipose tissue. The adipose-derived stem cells may be preadipocytes, stromal cells, multipotent adipose-derived cells, or adipose derived adult stem cells, but are not limited thereto.

The 'stem cell culture medium' refers to stem cells cultured in a medium capable of supporting stem cell growth and survival under in vitro culture conditions, and the present invention is a human stem cell culture medium that promotes exosome production (STEMATURE-Exosome ^TM ) includes

The exosome-promoted human stem cell culture medium (STEMATURE-Exosome ^TM ) is treated with a complex composition containing human mesenchymal stem cells, human platelet lysate, serum replacement, sodium pyruvate, insulin, transferrin and sodium selenite. It is a human stem cell culture medium (Human Stem Cell Conditioned Media) obtained by a method for promoting the production of exosomes and/or extracellular vesicles, including the step of culturing in a cultured medium.

The manufacturing method of the human stem cell culture medium in which the exosome production is promoted is specifically described in Korean Patent No. 10-2284517, and may be introduced as a reference in the present invention.

In one embodiment, the concentration of the serum replacement agent may be in the range of 5 to 15%, preferably 7 to 12%, more preferably 9 to 11%, and most preferably 10%.

In one embodiment, the concentration of sodium pyruvate may be in the range of 500 to 2000 μM, preferably 700 to 1600 μM, more preferably 900 to 1300 μM, and most preferably 1000 μM.

In one embodiment, the concentration of the insulin may be in the range of 0.86 to 3.44 μM, preferably 0.96 to 2.44 μM, more preferably 1.06 to 2.08 μM, and most preferably 1.72 μM.

In one embodiment, the concentration of the transferrin may be in the range of 0.03 to 0.14 μM, preferably 0.04 to 0.13 μM, more preferably 0.05 to 0.12 μM, and most preferably 0.07 μM.

In one embodiment, the concentration of sodium selenite may be in the range of 0.01 to 0.08 μM, preferably 0.02 to 0.07 μM, more preferably 0.03 to 0.06 μM, and most preferably 0.04 μM.

The composition of the present invention is purified water, glycerin, butylene glycol, glycerose, dipropylene glycol, cetyl ethyl hexanoate, triethyl hexanoin, sucrose oleate, oleander leaf extract, allantoin, glyceryl acrylate / Acrylic acid copolymer, polyglyceryl-10 dioleate, pentylene glycol, caprylyl glycol, 1,2-hexanediol, and further comprising at least one selected from the group consisting of EDTA can do.

Specifically, the composition of the present invention may include additional components in addition to the human stem cell culture medium (STEMATURE-Exosome ^TM ). For example, purified water, glycerin, butylene glycol, glycerin, dipropylene glycol, cetyl ethyl hexanoate, triethyl hexanoin, sucrose oleate, oleander leaf extract, allantoin, glyceryl acrylate/acrylic It may include acid copolymer, polyglyceryl-10 dioleate, pentylene glycol, caprylyl glycol, 1,2-hexanediol, and disodium EDTA.

The human stem cell culture medium (STEMATURE-Exosome ^TM ) is 15-25% (w / w), specifically 18-22% (w / w), more specifically 20% (w / w) based on the total weight of the composition may be included as

The purified water may be included in 40-50% (w/w), specifically 43-48% (w/w), more specifically 47-48% (w/w) based on the total weight of the composition.

Additionally, the composition of the present invention may include one or more components selected from the group consisting of glycerin, butylene glycol, glycereth-26, dipropylene glycol, cetylethylhexanoate, and triethylhexanoin.

The glycerin is included in 10-20% (w / w), specifically 12-15% (w / w) based on the total weight of the composition, and butylene glycol is 5-15% (based on the total weight of the composition) w/w), specifically 8-12% (w/w), and glycereth-26 is 1-5% (w/w), specifically 2-4% (w/w) based on the total weight of the composition w), and dipropylene glycol is included in 1-5% (w/w), specifically 1-3% (w/w) based on the total weight of the composition, cetylethylhexanoate, tri Ethylhexanoin may be included in 0.5-3% (w/w), specifically 0.5-2% (w/w), based on the total weight of the composition.

In addition, additionally sucrose oleate, sucrose leaf extract, allantoin, glyceryl acrylate / acrylic acid copolymer, polyglyceryl-10 dioleate, pentylene glycol, caprylyl glycol, 1,2-hexanedi All and/or disodium EDIT may be included in an amount of 0.001 to 0.9% (w/w), respectively, based on the total weight of the composition.

Components included in the composition of the present invention and specific contents of each are as follows.

The present invention is a composition for improving skin beauty, and the skin is composed of an epidermis, a dermis, and a subcutaneous tissue. The epidermis, the outer layer of the skin, is composed of stratified squamous epithelium and acts as a protective barrier of the body against the external environment. From the outside, the epidermis is divided into stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum and stratum basale. The dermis is a layer between the epidermis and subcutaneous tissue, and it is divided into a papillary dermis and a reticular dermis. Sensory nerves, fibroblasts and macrophages are present and occupy the largest portion of the skin.

The dermis consists mainly of collagen and elastin fibers, which serve to support the skin. Therefore, when such a problem occurs in the dermis, wrinkles are formed and skin elasticity is lost, leading to skin aging.

The composition according to the present invention can be used to improve skin beauty, for example, improve skin moisture, improve skin tone, improve skin whitening due to hyperpigmentation, improve wrinkles around eyes, improve skin elasticity and fatigue, or improve skin density.

The composition may be a cosmetic composition, and may be prepared in the form of a lotion, skin, toner, essence, ampoule, emulsion, gel, water, solid, suspension, foam, aerosol, foam, pack, patch or cream, etc. It is not limited.

In some cases, it may further include one or more cosmetically acceptable carriers, for example, oil and fat components, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, ultraviolet absorbers, preservatives, A disinfectant, an antioxidant, a plant extract, a pH adjuster, alcohol, a colorant, a fragrance, a blood circulation promoter, or a cooling agent may be appropriately mixed, but is not limited thereto.

Example

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples.

manufacturing example. Composition specifics

A sample containing the composition according to the present invention is named 'STEMATURE-Exosome ^TM 20% ampoule'.

[STEMATURE-Exosome ^TM 20% Ampoule]

The purpose of this study was to verify the clinical efficacy of 'STEMATURE-Exosome ^TM 20% Ampoule' on the skin. The specific research method is as follows.

(1) Clinical study subjects: 10 women in their 40s and 50s

(2) Research sample: STEMATURE-Exosome ^TM 20% ampoule 1 type

① For 4 weeks (28 days), 3 mL twice a day in the morning and evening was applied to the entire face.

② After applying the sample, it was left untouched so that it was absorbed.

(3) Clinical trial period: September 9 - October 22, 2021

(4) skin measurement

① When carrying out skin measurement according to the items, the study subjects wash their face, which is the measurement site, and induce skin stabilization in an environment of constant temperature and humidity (temperature 22±2℃, humidity 40-60%) for 30 minutes, and then perform skin measurement. did.

Example: Results

1. Changes in facial skin hydration

① There was a significant increase (p<.05) in the moisture content of the skin by 8.9%.

② The amount of transdermal water loss decreased by 6.2%.

2. Changes in pigmentation of facial skin (Fig. 1)

① Skin tone increased by 0.3%.

② Melanin pigment was significantly decreased (p<.05) by 3.1%.

3. Changes in elasticity of facial skin (Fig. 2)

① There was a significant decrease (p<.05) of wrinkles around the eyes by 7.4%.

② Skin fatigue decreased by 3.8%.

4. Changes in skin density: Confirmation results are shown in FIG. 3 .

From the above data, the following can be confirmed.

(1) 'STEMATURE-Exosome ^TM 20% Ampoule' is effective in moisturizing the skin.

(2) 'STEMATURE-Exosome ^TM 20% Ampoule' helps to brighten skin tone.

(3) 'STEMATURE-Exosome ^TM 20% Ampoule' has the effect of improving whitening in hyperpigmentation such as spots and freckles.

(4) 'STEMATURE-Exosome ^TM 20% Ampoule' is effective in improving wrinkles around the eyes.

(5) 'STEMATURE-Exosome ^TM 20% Ampoule' helps improve skin elasticity and fatigue.

(6) 'STEMATURE-Exosome ^TM 20% Ampoule' helps to improve skin density.

As described above in detail a specific part of the content of the present invention, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and the scope of the present invention is not limited thereby It will be obvious. Accordingly, it is intended that the substantial scope of the present invention be defined by the appended claims and their equivalents.

Claims (5)

As a composition for improving skin beauty,
47-48% by weight of purified water based on the total weight of the composition;
20% by weight of human stem cell culture medium containing exosomes,
12-15% by weight of glycerin,
butylene glycol 8-12 wt%,
Glycereth-26 2-4% by weight,
1-3 wt% of dipropylene glycol,
0.5-2 wt% of cetylethylhexanoate,
0.5-2% by weight of triethylhexanoin,
sucrose oleate, sucrose leaf extract, allantoin, glyceryl acrylate/acrylic acid copolymer, polyglyceryl-10 dioleate, pentylene glycol, caprylyl glycol, 1,2-hexanediol and diol A composition for improving skin beauty, each comprising 0.001 to 0.9 wt% of sodium EDI.
delete delete According to claim 1, wherein the exosome-containing human stem cell culture medium is human mesenchymal stem cells human platelet lysate, a serum substitute, sodium pyruvate, insulin, transferrin and sodium selenite in the treated medium with a complex composition comprising A composition for improving skin beauty comprising a human stem cell culture medium (Human Stem Cell Conditioned Media) obtained by a method for promoting generation of exosomes and/or extracellular vesicles, comprising the step of culturing.
According to claim 1, wherein the skin beauty improvement is skin moisturizing improvement, skin tone improvement, skin whitening improvement by hyperpigmentation, wrinkles around the eyes, skin elasticity improvement or skin density improvement, including improvement of skin density, improvement composition.

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