KR102423351B1 - Portable radioactivity test apparatus and method using bio sensor - Google Patents

Abstract

A portable radioactivity testing apparatus and method using a biosensor are provided. A portable radioactivity test apparatus according to an embodiment of the present invention includes: a biosensor including a transistor including biomolecules; a data generator configured to generate data by measuring the current output from the biosensor; a data storage unit for storing data before the biosensor is exposed to a measurement environment; a data processing unit that compares the data generated by the data generation unit with the data stored in the data storage unit to determine the presence or absence of radioactivity and the influence of radioactivity in the measurement environment; and an external frame configured to accommodate the biosensor, the data generation unit, the data storage unit, and the data processing unit.

Description

Portable radioactivity test apparatus and method using bio sensor

The present invention relates to a portable radioactivity test device, which is not a method of measuring the level of radioactivity, but can sense the effect of radioactivity present inside the measurement target, including a biosensor that uses biological entities as an index of influence on radioactivity It relates to a radioactivity testing apparatus and method.

The need for a portable sensor for food inspection is required at a time when anxiety about food safety is growing due to a radiation accident in Japan. Various technologies have been developed.

However, in the case of gamma rays, which have good permeability to the human body, it is possible to measure them with a conventional portable radiation detector. However, in the case of alpha and beta rays emitted from radioactive substances such as strontium and plutonium, which can have a fatal effect on the human body, it is difficult to penetrate the food itself when included in food, so it is theoretically impossible to measure with the existing portable surface radiation dose measuring device. do. In the case of the alpha rays and beta rays, if they occur from the natural environment or the ground, it is possible to shield them, but when alpha rays and beta rays are introduced into the body in a state of being contained in food, internal exposure may occur, resulting in a fatal risk.

The present invention aims to develop a new type of radiation sensor for food inspection that uses a biosensor using a transistor as a radiation measuring element to indirectly measure the effect on the human body.

The present invention provides a new type of radiographic examination apparatus and method for indirectly measuring the effect on the human body by using a biosensor used as an index of influence on radioactivity of biological entities.

A portable radioactivity test apparatus according to an embodiment of the present invention includes: a biosensor including a transistor including biomolecules; a data generator configured to generate data by measuring the current output from the biosensor; a data storage unit for storing data before the biosensor is exposed to a measurement environment; a data processing unit that compares the data generated by the data generation unit with the data stored in the data storage unit to determine the presence or absence of radioactivity and the influence of radioactivity in the measurement environment; and an external frame configured to accommodate the biosensor, the data generation unit, the data storage unit, and the data processing unit.

A portable radioactivity test method according to an embodiment of the present invention is a portable radioactivity test method using a biosensor, wherein the biosensor includes a transistor including biomolecules, and the method comprises: exposing the biosensor to a measurement environment generating first measurement data of the previous biosensor, and storing the generated first measurement data in a data storage unit; exposing the biosensor to the measurement environment and generating second measurement data of the biosensor; and comparing the generated second measurement data with the first measurement data stored in the data storage unit to determine the presence or absence of radioactivity and the radioactivity influence of the measurement environment.

A portable radioactivity test apparatus and method using a biosensor according to an embodiment of the present invention uses a transistor for biomolecules susceptible to radiation to measure the effects of alpha-ray and beta-ray nuclides existing inside a measurement target on the human body with a portable sensor. It attaches to the sensor and observes changes in biomolecular properties inside the measurement target. To this end, an array-type biosensor is formed on a frame that can be inserted inside the measurement target, and the measured electrical data is compared with the data before sensing to indicate the effect of the biomolecules.

In addition, unlike conventional sensors that are highly affected by external radiation, it is an important difference to overcome the limitations of existing sensors in that they are injected inside the measurement target to measure the influence that can affect living things. Specifically, it is possible to measure the effect on the living body rather than a direct method of measuring the absolute amount of radiation.

1 is an exemplary diagram illustrating the structure of a portable radioactivity testing apparatus according to an embodiment of the present invention.
FIG. 2 shows the structure of a transistor included in the biosensor of the portable radioactivity test device of FIG. 1 .
3 is a flowchart of a radioactivity test method according to another embodiment of the present invention.

Hereinafter, preferred embodiments according to the present invention will be described in detail with reference to the accompanying drawings. DETAILED DESCRIPTION The detailed description set forth below in conjunction with the appended drawings is intended to describe exemplary embodiments of the present invention and is not intended to represent the only embodiments in which the present invention may be practiced. The following detailed description includes specific details in order to provide a thorough understanding of the present invention. However, one of ordinary skill in the art will recognize that the present invention may be practiced without these specific details. Specific terms used in the following description are provided to help the understanding of the present invention, and the use of these specific terms may be changed to other forms without departing from the technical spirit of the present invention.

1 is an exemplary diagram showing the structure of a portable radioactivity testing apparatus according to an embodiment of the present invention. FIG. 2 shows the structure of a transistor included in the biosensor of the portable radioactivity test device of FIG. 1 .

Referring to FIG. 1 , a portable radioactivity test apparatus 10 according to an embodiment of the present invention includes a biosensor 100 , a data generator 110 , a data processor 120 , a data storage unit 130 , and a power supply unit ( 140 ) and an outer frame 150 .

A portable radiological testing device according to embodiments may be entirely hardware, or may have aspects that are partly hardware and partly software. For example, the portable radioactivity test device of the present specification and each unit included therein may collectively refer to a device for exchanging data in a specific format and content in an electronic communication method and software related thereto. As used herein, terms such as “unit”, “module”, “server”, “system”, “device” or “terminal” refer to a combination of hardware and software driven by the hardware. it is intended to be For example, the hardware herein may be a data processing device including a central processing unit (CPU) or other processor. In addition, software driven by hardware may refer to a running process, an object, an executable file, a thread of execution, a program, and the like.

In addition, each part constituting the portable radiological testing device is not necessarily intended to refer to a separate physically distinct component. In FIG. 1, the in situ biosensor 100, the data generating unit 110, the data processing unit 120, and the data storage unit 130 are shown as separate blocks that are separated from each other, but this is a device constituting a portable radioactivity testing device. It is only functionally classified by the actions performed by the device. Accordingly, in some embodiments, the biosensor 100 , the data generator 110 , the data processor 120 , and the data storage 130 may be integrated in part or all in the same device, and at least one It may be implemented as a separate device physically separated from other units, or may be components communicatively connected to each other in a distributed computing environment.

The biosensor 100 may have an array structure arranged in one direction or a matrix. The biosensor 100 may include nano-sized biological entities such as DNA, proteins, and viruses, and the biosensor 100 may include a device for detecting changes in these biological entities as changes in current. have. For example, the biosensor 100 may be a field effect transistor (FET) configured to sense a DNA sequence.

Referring to FIG. 2 , the biosensor 100 includes a substrate 101 , a gate 102 , a source region 103 , a drain region 104 , a channel region 105 , an interlayer insulating layer 106 , and a biomolecule 107 . ), a sidewall 108 and a gate insulating film 109 .

The substrate 101 may be formed of any suitable semiconductor material, for example, silicon (Si), germanium (Ge), silicon germanium (SiGe), indium arsenide (InAs), silicon germanium (Sin), germanium tin (GeSn), silicon. germanium tin (SiGeSn), or any other III-V or II-VI semiconductors.

An interlayer insulating film 106 is formed on the substrate 101 . The interlayer insulating film 106 may be formed of a silicon oxide film (SiOx) or a silicon nitride film (SiNx), and may be formed of a single layer of an oxide film or a nitride film, or a multilayer of two or more layers.

A source 103 and a drain 104 are formed on the interlayer insulating layer 106 to be spaced apart from each other with a channel region 105 interposed therebetween. The source region 3 may include a semiconductor material doped with an n-type dopant species, and the drain region 4 may include a semiconductor material doped with a p-type dopant species, but is not limited thereto.

The gate insulating film 109 is formed on the region between the source 103 and the drain 104 of the substrate 101 , that is, the channel region 105 , and the gate 102 is formed on the gate insulating film 109 . The gate insulating film 109 is formed on the upper portion of the region located between the region in which the source 103 and the drain 104 are formed in the substrate 101 . The gate insulating layer 109 refers to a region including silicon oxide between the gate 102 and the substrate 101 , a high insulating material (High-K), and an insulating material that can be used in a field effect transistor.

The gate 102 may be formed on a portion of the gate insulating layer 109 to electrically control an energy band of a portion of the channel region 105 .

The biomolecules 107 are located on the side of the gate 102 . That is, the biomolecules 107 are formed on another portion of the upper portion of the gate insulating layer 109 so as not to overlap the gate 102 . Biomolecules 107 may electrically affect energy bands in other channel regions 105 that are not controlled by gate 102 . The biomolecules 107 may be formed by bonding the biomolecules 107 to the surface of the gate insulating layer 109 exposed by etching the gate 102 after a portion of the gate 102 is etched by a hard mask.

The biomolecule 107 can be appropriately selected according to the type of the detection target substance. For example, the biomolecule 107 may be one or more of DNA, RNA, nucleic acid analogs, proteins, peptides, amino acids, ligands, antibody-antigen substances, glycostructures, organic/inorganic compounds, vitamins, drugs, and enzymes. may include

In addition, the biomolecule 107 may be a biological medium sensitive to radiation. That is, in the biomolecule 107, at least one of the binding between the internal DNA bases, the amino acid binding, and the modification of the protein may occur according to the radiation exposure. In particular, DNA modifications such as cleavage of DNA base bonds by direct radiation or free radicalization of water molecules and the binding of two adjacent thymines on one base strand occur in DNA. will do Accordingly, the biomolecule 107 may include a DNA nucleotide sequence mainly including a thymine base, and thus may exhibit a characteristic change sensitive to radiation exposure. Current characteristics of the biosensor 100 of the present invention may vary according to changes in the biomolecules 107 , and the biosensor 100 may function as a sensor using this phenomenon. Since the portable radioactivity test device according to an embodiment of the present invention uses biomolecules rather than absolute values of radioactivity, it is possible to measure the influence that it can have on a living body.

Here, the channel region 105 disposed under the gate insulating film 109 is divided into a part in which the energy band is controlled by the effect of the charge generated by the bio-molecules 107 and a part in which the energy band is controlled by the gate 102 . However, the amount of current generated during measurement by the gate voltage by the gate 102 can be freely adjusted. Here, if a part of the channel region 105 is electrically controlled by the gate 102 and the remaining part is electrically controlled due to the effect of the charge amount of the bio-molecules 107, a feedback phenomenon occurs, and the bio-molecules 107 The current flowing through the channel region 105 is rapidly changed according to the amount of charge. Accordingly, the current is controlled only by the effect of the charge amount of the bio-molecules 107 , and a minute change of the bio-molecules 107 can be detected.

A sidewall 108 is formed between the gate 102 and the bio-molecules 107 to electrically insulate the gate 102 and the bio-molecules 107 . The sidewall 108 may be formed of a silicon oxide film (SiOx) or a silicon nitride film (SiNx), and may be formed of a single layer of an oxide film or a nitride film, or a multilayer of two or more layers. The sidewall 108 is formed for the electrical separation of the gate 102 and the biomolecule 107 , and different portions of the channel region 105 are connected to the gate 102 and the biomolecule 107 by the sidewall 108 . Because it is easy to control electrically due to the influence of

The data generator 110 is electrically connected to each part of the array of the biosensor 100 to measure the current output from the biosensor 100 to generate data. The data generator 110 may include a noise filter and an amplifier circuit to ensure reliability of the generated data. The data generated by the data generating unit 110 may be provided to the data storage unit 130 and the data processing unit 120 .

The data storage unit 130 may store the data generated by the data generation unit 110 . The data storage unit 130 may store data prior to exposure to the measurement environment, and this previous data may be used as comparative data for determining whether or not radiation exposure is present. In addition, the data storage unit 130 may further provide a function of temporarily storing temporary data generated when the data generation unit 110 and the data processing unit 120 generate and process data.

The data processing unit 120 may compare the measured data with the data stored in the data storage unit 140 that stores the data before exposure to the measurement environment to determine the presence of radioactivity inside the measurement target and the influence of the radioactivity. As the portable radioactivity test device 10 is exposed to the measurement environment, the biomolecules 107 included in the biosensor 110 are affected by the radiation contained in the measurement environment. Accordingly, the output current value of the biosensor 110 generated by the data generator 110 may be changed. The data processing unit 120 determines the influence of radioactivity by comparing the data before and after exposure to the measurement environment. For example, the data processing unit 120 may determine that radioactivity does not exist when a difference value between data before and after exposure to the measurement environment is less than or equal to a predetermined reference value. In addition, the data processing unit 120 determines that radioactivity is present when the difference value between data before and after exposure to the measurement environment exceeds a predetermined reference value, and may determine the influence of radioactivity in proportion to the difference value.

The power supply unit 140 supplies power to enable the operation of the portable radioactivity test device 10 using the biosensor. The power supply unit 140 may be a power adapter for supplying DC power to the portable radiation test device 10, but is not limited thereto. The power supply unit 140 may be configured as a portable battery, and thus the portability of the portable radioactivity testing device 10 may be further increased.

The outer frame 150 may accommodate the components of the portable radioactivity testing device 10 . The outer frame 150 is manufactured in a structure for easy insertion into the radioactivity measurement target. That is, since the radioactivity test is performed after being inserted into the measurement target, interference with the test by external radioactivity can be minimized. Here, the radioactivity measurement target may be food, and the outer frame 150 may be configured in a form that can be inserted into the food. Also, the external frame 150 may block external factors other than radiation from flowing into the biosensor 100 . That is, the biosensor 100 may be in a sealed state in which contact with external factors is blocked by the external frame 150 until it is used as a sensor. The outer frame 150 has an insulating configuration to block external electrical factors, a shielding configuration to block light of a specific wavelength, a heat dissipation configuration such as a heat pipe to prevent damage to the biomolecules 107 due to high temperature, a constant It may include at least one of a constant humidity configuration to maintain the humidity in the range.

The portable radioactivity test apparatus 10 using a biosensor according to an embodiment of the present invention uses a transistor to convert biomolecules vulnerable to radiation to measure the effects of alpha-ray and beta-ray nuclides existing inside a measurement target on the human body with a portable sensor. It is attached to the used sensor and the change of the biomolecular properties inside the measurement target is observed. To this end, an array-type biosensor is formed on a frame that can be inserted inside the measurement target, and the measured electrical data is compared with the data before sensing to indicate the effect of the biomolecules.

A portable radioactivity test apparatus and method using a biosensor according to an embodiment of the present invention uses a transistor for biomolecules susceptible to radiation to measure the effects of alpha-ray and beta-ray nuclides existing inside a measurement target on the human body with a portable sensor. It attaches to the sensor and observes changes in biomolecular properties inside the measurement target. To this end, an array-type biosensor is formed on a frame that can be inserted inside the measurement target, and the measured electrical data is compared with the data before sensing to indicate the effect of the biomolecules.

In addition, unlike conventional sensors that are highly affected by external radiation, it is an important difference that can overcome the limitations of existing sensors in that they are injected inside the measurement target to measure the influence that can affect living things. Specifically, it is possible to measure the effect on the living body rather than a direct method of measuring the absolute amount of radiation.

3 is a portable radioactivity test method using a biosensor according to another embodiment of the present invention.

The portable radioactivity test method of FIG. 3 can be performed using a portable radioactivity test apparatus using a biosensor according to FIGS. 1 and 2, and in the description of this embodiment, a portable radioactivity test apparatus using the biosensor of FIGS. 1 and 2 The description may be referred to.

Referring to FIG. 3 , a portable radioactivity test method using a biosensor according to another embodiment of the present invention is a portable radioactivity test method using a biosensor, wherein the biosensor includes a transistor including biomolecules, the method comprising: : generating first measurement data of the biosensor before the biosensor is exposed to a measurement environment, and storing the generated first measurement data in a data storage unit (S100); exposing the biosensor to the measurement environment and generating second measurement data of the biosensor (S110); and comparing the generated second measurement data with the first measurement data stored in the data storage unit to determine the presence or absence of radiation and the radiation influence of the measurement environment (S120).

First, first measurement data of the biosensor is generated before the biosensor is exposed to the measurement environment, and the generated first measurement data is stored in the data storage unit (S100).

This step ( S100 ) corresponds to a step of generating comparison data before exposure to a measurement environment and a target. That is, before the biosensor is exposed to the measurement environment, first measurement data (comparative data) of the biosensor is generated and stored in the data storage unit.

Next, the biosensor is exposed to the measurement environment, second measurement data of the biosensor is generated (S110), and the generated second measurement data is compared with the first measurement data stored in the data storage unit to measure the measurement. It determines the presence or absence of radioactivity in the environment and the influence of radioactivity (S120).

Here, the biomolecules included in the biosensor may be changed by radioactivity present in the measurement environment, so that the characteristics of the current output from the biosensor may vary. Data before exposure to the measurement environment (first measurement data) and data measured after exposure to the measurement environment (second measurement data) may be compared to determine the presence or absence of radioactivity inside the measurement target and the influence of radioactivity. Here, the biomolecule is a DNA nucleotide sequence, and a change in binding of the DNA nucleotide sequence may occur due to the radioactivity.

In the step (S120) of determining the presence or absence of radioactivity and the influence of radiation in the measurement environment, when the difference between the second measurement data and the first measurement data stored in the data storage unit exceeds a predetermined reference value, the radiation in the measurement environment is It is determined that there is, and the radiation influence can be determined according to the magnitude of the difference.

In the test method according to this embodiment, in order to measure the effects of alpha-ray and beta-ray nuclides existing inside the measurement target on the human body with a portable sensor, biomolecules susceptible to radiation are attached to a sensor using a transistor, and biomolecules inside the measurement target are attached to the sensor. Observe the characteristic change. To this end, an array-type biosensor is formed on a frame that can be inserted inside the measurement target, and the measured electrical data is compared with the data before sensing to indicate the effect of the biomolecules. In addition, unlike conventional sensors that are highly affected by external radiation, it is an important difference to overcome the limitations of existing sensors in that they are injected inside the measurement target to measure the influence that can affect living things. Specifically, it is possible to measure the effect on the living body rather than a direct method of measuring the absolute amount of radiation.

Although described above with reference to the embodiments, the present invention should not be construed as being limited by these embodiments or drawings, and those skilled in the art will appreciate the spirit and scope of the present invention described in the claims below. It will be understood that various modifications and variations of the present invention can be made without departing from the scope of the present invention.

10: portable radioactivity testing device
100: biosensor
110: data generation unit
120: data processing unit
130: data storage unit
140: power unit
150: outer frame

Claims (10)

A channel region, a source region and a drain region disposed with the channel region interposed therebetween, a gate insulating layer disposed on the channel region, a gate formed on a portion of the upper portion of the gate insulating layer, and an upper portion of the gate insulating layer so as not to overlap the gate a biosensor comprising a transistor comprising biomolecules positioned therein and a sidewall electrically insulating the gate and the biomolecules;
a data generator configured to generate data by measuring the current output from the biosensor;
a data storage unit for storing data before the biosensor is exposed to a measurement environment;
a data processing unit that compares the data generated by the data generation unit with the data stored in the data storage unit to determine the presence or absence of radioactivity and the influence of radioactivity in the measurement environment; and
and an external frame configured to accommodate the biosensor, the data generation unit, the data storage unit, and the data processing unit. The method of claim 1,
The biomolecules are changed by radioactivity present in the measurement environment, so that the current characteristics output from the biosensor are different,
The data processing unit determines the change in current characteristics of the biomolecules to determine the presence or absence of radioactivity and the influence of radioactivity in the measurement environment. 3. The method of claim 2,
The biomolecule is a DNA base sequence,
A portable radioactivity test device, characterized in that a change in the binding of the DNA base sequence occurs by the radioactivity. The method of claim 1,
The data processing unit determines that radioactivity exists in the measurement environment when the difference between the data generated by the data generation unit and the data stored in the data storage unit exceeds a predetermined reference value,
The data processing unit portable radioactivity test device, characterized in that for determining the influence of the radioactivity according to the magnitude of the difference. The method of claim 1,
The external frame is a portable radioactivity testing device, characterized in that sealing the biosensor to block contact with external factors until the state before the biosensor is used as a sensor. 6. The method of claim 5,
The outer frame has an insulating configuration to block external electrical factors, a shielding configuration to block light of a specific wavelength, a heat dissipation configuration to prevent damage to the biomolecules due to high temperature, and constant humidity to maintain a certain range of humidity Portable radioactivity testing device comprising at least one of the components. A portable radioactivity test method using a biosensor, wherein the biosensor is formed in a channel region, a source region and a drain region disposed with the channel region interposed therebetween, a gate insulating film positioned on the channel region, and a portion of the upper portion of the gate insulating film A transistor comprising: a transistor comprising a gate, a biomolecule positioned on the gate insulating layer so as not to overlap the gate, and a sidewall electrically insulating the gate and the biomolecule, the method comprising:
generating first measurement data of the biosensor before the biosensor is exposed to a measurement environment, and storing the generated first measurement data in a data storage unit;
exposing the biosensor to the measurement environment and generating second measurement data of the biosensor; and
A portable radioactivity test method comprising the step of comparing the generated second measured data with the first measured data stored in the data storage unit to determine the presence or absence of radioactivity and radioactivity influence of the measuring environment. 8. The method of claim 7,
The biomolecule is a portable radioactivity test method, characterized in that the change occurs by the radioactivity present in the measurement environment, characterized in that the current output from the biosensor is different. 9. The method of claim 8,
The biomolecule is a DNA base sequence,
A portable radioactivity test method, characterized in that a change in the binding of the DNA base sequence occurs by the radioactivity. 8. The method of claim 7,
The step of determining the presence or absence of radioactivity and the radioactive influence of the measurement environment,
When the difference between the second measurement data and the first measurement data stored in the data storage unit exceeds a predetermined reference value, it is determined that radiation is present in the measurement environment, and the radiation influence is determined according to the magnitude of the difference Portable radioactivity testing method, characterized in that.

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