KR102388736B1 - Method for removing residual pesticide in natural plant extract by three stage treatment - Google Patents

Abstract

The present invention comprises the steps of: treating a glycerin fatty acid ester; adding edible oil; and performing ultrafiltration. The present invention relates to a method for manufacturing a ginseng concentrate with reduced fat-soluble residual pesticide content, and a ginseng concentrate with reduced fat-soluble residual pesticide content produced by the method.

Description

Method for removing residual pesticide in natural plant extract by three stage treatment

The present invention comprises the steps of (1) preparing a ginseng extract by adding water and glycerin fatty acid ester to ginseng and then extracting; (2) stirring after adding edible oil to the ginseng extract prepared in step (1); (3) removing the upper oil layer from the ginseng oil liquid separated into an upper oil layer and a lower ginseng extract layer by allowing the stirred ginseng oil liquid of step (2) to stand; (4) ultra-filtering the ginseng extract layer from which the oil layer of step (3) has been removed; and (5) a method for producing a ginseng concentrate with reduced pesticide content, characterized in that it comprises the step of concentrating the ultra-filtered ginseng extract of step (4), and ginseng with reduced pesticide content prepared by the method It's about the concentrate.

Pesticides refer to all drugs used for the purpose of disinfecting the soil and seeds of farmland for growing crops, protecting crops from pests and pests occurring during crop cultivation, and controlling diseases and pests of stored agricultural products.

Although there are some differences depending on the type, there is a certain degree of toxicity, so excessive use of pesticides may cause adverse effects such as damage to crops or excessive pesticide residues in agricultural products, contaminating our food and polluting the environment. is likely to cause

In particular, the Ministry of Food and Drug Safety considers agricultural products as food and sets acceptable standards for pesticide residues in agricultural products.

Ginseng is one of Korea's representative economic crops and is grown in the same place for 4 to 6 years, so pesticides are inevitably used to prevent pests and diseases. The types of pesticides required for cultivation are set by the government, and residue tolerance standards are set so that safe products can be distributed.

However, consumers who want to be safe from pesticide residues and environmental pollutants have a growing preference for organic products, but the distribution of organic ginseng is very small and the price is too high. In addition, there are differences in the permissible standards for pesticide residues by country, and the United States and Japan are managed at a lower level than Korea's permissible standards, which is a very big obstacle when trying to export.

Korean Patent No. 1210522 discloses a method for removing pesticide residues in ginseng, and Korean Patent Registration No. 1638544 discloses a method for removing pesticide residues from plant powder, but residues in natural plant extracts by the three-step treatment of the present invention It is different from the method of removing pesticides.

The present invention has been derived from the above needs, and an object of the present invention is to optimize the treatment process to produce a ginseng concentrate that can effectively reduce the residual pesticide content in the ginseng concentrate while maintaining the quality of the ginseng concentrate as much as possible. , to provide a method for producing a ginseng concentrate that maximizes the reduction of harmful components such as phthalates and residual pesticides.

In order to solve the above problems, the present invention comprises the steps of (1) preparing a ginseng extract by adding water and glycerin fatty acid ester to ginseng and then extracting; (2) stirring after adding edible oil to the ginseng extract prepared in step (1); (3) removing the upper oil layer from the ginseng oil liquid separated into an upper oil layer and a lower ginseng extract layer by allowing the stirred ginseng oil liquid of step (2) to stand; (4) ultra-filtering the ginseng extract layer from which the oil layer of step (3) has been removed; And (5) provides a method for producing a ginseng concentrate with reduced pesticide content, characterized in that it comprises the step of concentrating the ultra-filtered ginseng extract of the step (4).

In addition, the present invention provides a ginseng concentrate with reduced pesticide content prepared by the above method.

The ginseng concentrate processed by the method of the present invention can completely remove 320 kinds of pesticide components and also remove phthalates, so consumers can safely consume it, and the characteristic bitter taste of ginseng is reduced and due to its soft taste. It has the advantage of being easy to consume.

In order to achieve the object of the present invention, the present invention

(1) preparing a ginseng extract by adding water and glycerin fatty acid ester to ginseng and extracting;

(2) stirring after adding edible oil to the ginseng extract prepared in step (1);

(3) removing the upper oil layer from the ginseng oil liquid separated into an upper oil layer and a lower ginseng extract layer by allowing the stirred ginseng oil liquid of step (2) to stand;

(4) ultra-filtering the ginseng extract layer from which the oil layer of step (3) has been removed; and

(5) provides a method for producing a ginseng concentrate with reduced pesticide content, characterized in that it comprises the step of concentrating the ultra-filtered ginseng extract of the step (4).

In the method for producing a ginseng concentrate of the present invention, the ginseng may be one or more ginseng selected from the group consisting of fresh ginseng, red ginseng, dried ginseng, white ginseng, black ginseng, Taegeuk ginseng, gok ginseng, wild ginseng, Jangnoe ginseng, wild ginseng cultured root, sprout ginseng, and wild ginseng. , but not limited thereto.

In addition, in place of the ginseng, one or more materials selected from the group consisting of herbal medicines, vegetables and fruits may be used, and more specifically, deodeok, sagebrush, hwangchil, mul honey, antler, nokak, cordyceps, garlic, gold, cabbage, pumpkin, At least one material selected from the group consisting of pomegranate, plum, and jujube may be used, but the present invention is not limited thereto.

In addition, in the method for producing a ginseng concentrate of the present invention, the ginseng extract of step (1) is preferably 60 to 80 ° C by adding 450 to 550 mL of water and 0.8 to 1.2 g of glycerin fatty acid ester to 80 to 120 g of ginseng. 40 to 45 Brix of ginseng extract can be prepared by extraction for 2 to 4 hours at A ginseng extract of ~45 Brix can be prepared.

In addition, in the method for producing the ginseng concentrate of the present invention, the step (2) is preferably after adding canola oil to the ginseng extract in a volume ratio of 8 to 9: 1 to 2 and then stirring at 30 to 35° C. for 30 to 60 minutes. More preferably, after adding canola oil to the ginseng extract in a volume ratio of 8.5:1.5, the mixture may be stirred at 30 to 35° C. for 30 to 60 minutes.

In addition, when the oil is added, in order to further improve the flavor and taste of the ginseng concentrate, coconut oil and date palm oil may be additionally added. More specifically, 8 to 9 canola oil, coconut oil and date palm oil are added to the ginseng extract. :0.8~1.2:0.2~0.4:0.1~0.3 After adding in a volume ratio, it can be stirred at 30~35℃ for 30~60 minutes, more preferably canola oil, coconut oil and date palm oil in ginseng extract 8.5: After adding in a volume ratio of 1:0.3:0.2, it can be stirred at 30-35° C. for 30-60 minutes.

In addition, in the method for producing a ginseng concentrate of the present invention, in step (3), the stirred ginseng oil solution is preferably left at 15 to 25° C. for 20 to 28 hours, the upper oil layer, and the lower ginseng extract layer The oil layer of the upper layer can be removed from the ginseng oil liquid separated by The upper oil layer can be removed from the oil liquid.

In addition, in the method for producing a ginseng concentrate of the present invention, the step (5) may preferably concentrate the ultra-filtered ginseng extract at 60 to 65° C. for 2 to 4 hours to 65 to 70 Brix, more preferably can concentrate the ultra-filtered ginseng extract to 65-70 Brix for 3 hours at 60-65°C.

The method for producing a ginseng concentrate with reduced pesticide content of the present invention, more specifically

(1) preparing a 40-45 Brix ginseng extract by adding 450-550 mL of water and 0.8-1.2 g of glycerin fatty acid ester to 80-120 g of ginseng and extracting it at 60-80°C for 2-4 hours;

(2) adding canola oil to the ginseng extract prepared in step (1) in a volume ratio of 8 to 9: 1 to 2, followed by stirring at 30 to 35° C. for 30 to 60 minutes;

(3) The stirred ginseng oil solution of step (2) was left at 15-25° C. for 20-28 hours, and the upper oil layer was separated from the ginseng oil liquid separated into the upper oil layer and the lower ginseng extract layer. removing;

(4) ultra-filtering the ginseng extract layer from which the oil layer of step (3) has been removed; and

(5) may include the step of concentrating the ultra-filtered ginseng extract of step (4) to 65-70 Brix at 60-65° C. for 2-4 hours,

more specifically

(1) preparing a ginseng extract of 40-45 Brix by adding 500 mL of water and 1 g of glycerin fatty acid ester to 100 g of ginseng and extracting it at 70° C. for 3 hours;

(2) adding canola oil to the ginseng extract prepared in step (1) in a volume ratio of 8.5:1.5 and stirring at 30 to 35° C. for 30 to 60 minutes;

(3) removing the upper oil layer from the ginseng oil liquid separated into an upper oil layer and a lower ginseng extract layer by allowing the stirred ginseng oil solution of step (2) to stand at 20° C. for 24 hours;

(4) ultra-filtering the ginseng extract layer from which the oil layer of step (3) has been removed; and

(5) It may include the step of concentrating the ultra-filtered ginseng extract of step (4) to 65-70 Brix at 60-65° C. for 3 hours.

The present invention also provides a ginseng concentrate with reduced pesticide content prepared by the above method.

Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples only illustrate the present invention, and the content of the present invention is not limited to the following examples.

Preparation Example 1. Red ginseng concentrate (glycerin fatty acid ester-edible oil-ultrafiltration treatment)

(1) 500 mL of purified water and 1 g of glycerin fatty acid esters (Glycerin Esters of Fatty Acids, GEFA) were added to 100 g of red ginseng, and extracted at 70° C. for 3 hours to prepare a 40-45 Brix red ginseng extract.

(2) After adding canola oil to the red ginseng extract prepared in step (1) at a volume ratio of 9:1, the mixture was circulated with a pump and stirred at 30 to 35° C. at 45 rpm for 30 to 60 minutes.

(3) The stirred red ginseng oil solution of step (2) was left at 20° C. for 24 hours, and the upper oil layer was removed from the red ginseng oil solution separated into an upper oil layer and a lower red ginseng extract layer.

(4) The red ginseng extract layer from which the oil layer of step (3) was removed was ultra-filtered.

At this time, the membrane used in the ultrafiltration used at least one of cellulose acetate and polyethersulfone, the membrane pore size was 0.1 to 0.3 μm, the feed pressure was 5 to 20 bar, and it was carried out at room temperature. The ultrafiltration process was first filtered through a 50 μm sieve, then secondarily filtered through a 30 μm sieve, cooled to 20° C., filtered thirdly through a 20 μm sieve, and then cooled to 2° C. again, 0.1 A multi-stage filtration step of filtration through a sieve of μm was performed.

(5) The ultra-filtered red ginseng extract of step (4) was concentrated at 60-65° C. under a pressure of 600-670 mmHg for 3 hours to prepare a 65-70 Brix red ginseng concentrate.

Preparation Example 2. Red ginseng concentrate (glycerin fatty acid ester-edible oil-ultrafiltration treatment)

(1) 500 mL of purified water and 1 g of glycerin fatty acid esters (Glycerin Esters of Fatty Acids, GEFA) were added to 100 g of red ginseng, and extracted at 70° C. for 3 hours to prepare a 40-45 Brix red ginseng extract.

(2) After adding canola oil, coconut oil, and date palm oil in a volume ratio of 8.5:1:0.3:0.2 to the red ginseng extract prepared in step (1), circulating with a pump at 30-35°C at 45 rpm for 30- Stir for 60 minutes.

(3) The stirred red ginseng oil solution of step (2) was left at 20° C. for 24 hours, and the upper oil layer was removed from the red ginseng oil solution separated into an upper oil layer and a lower red ginseng extract layer.

(4) The red ginseng extract layer from which the oil layer of step (3) was removed was ultra-filtered.

At this time, the membrane used in the ultrafiltration used at least one of cellulose acetate and polyethersulfone, the membrane pore size was 0.1 to 0.3 μm, the feed pressure was 5 to 20 bar, and it was carried out at room temperature. The ultrafiltration process was first filtered through a 50 μm sieve, then secondarily filtered through a 30 μm sieve, cooled to 20° C., filtered thirdly through a 20 μm sieve, and then cooled to 2° C. again, 0.1 A multi-stage filtration step of filtration through a sieve of μm was performed.

(5) The ultra-filtered red ginseng extract of step (4) was concentrated at 60-65° C. under a pressure of 600-670 mmHg for 3 hours to prepare a 65-70 Brix red ginseng concentrate.

control group. Red Ginseng Concentrate

(1) 100 g of red ginseng was added with 500 mL of purified water and extracted at 70° C. for 3 hours to prepare a 40-45 Brix red ginseng extract.

(2) The red ginseng extract prepared in step (1) was concentrated at 60-65° C. at a pressure of 600-670 mmHg for 3 hours to prepare a 65-70 Brix red ginseng concentrate.

Comparative Example 1. Red ginseng concentrate (glycerin fatty acid ester treatment)

(1) 500 mL of purified water and 1 g of glycerin fatty acid esters (Glycerin Esters of Fatty Acids, GEFA) were added to 100 g of red ginseng, and extracted at 70° C. for 3 hours to prepare a 40-45 Brix red ginseng extract.

(2) The red ginseng extract prepared in step (1) was concentrated at 60-65° C. at a pressure of 600-670 mmHg for 3 hours to prepare a 65-70 Brix red ginseng concentrate.

Comparative Example 2. Red ginseng concentrate (cooking oil treatment)

(1) 100 g of red ginseng was added with 500 mL of purified water and extracted at 70° C. for 3 hours to prepare a 40-45 Brix red ginseng extract.

(2) After adding canola oil to the red ginseng extract prepared in step (1) in a volume ratio of 9:1, it was stirred at 20° C. at 45 rpm for 30 to 60 minutes while circulating with a pump.

(3) The stirred red ginseng oil solution of step (2) was left at 20° C. for 24 hours, and the upper oil layer was removed from the red ginseng oil solution separated into an upper oil layer and a lower red ginseng extract layer.

(4) The red ginseng extract layer from which the oil layer of step (3) was removed was concentrated at 60-65° C. under a pressure of 600-670 mmHg for 3 hours to prepare a 65-70 Brix red ginseng concentrate.

Comparative Example 3. Red ginseng concentrate (ultrafiltration treatment)

(1) 100 g of red ginseng was added with 500 mL of purified water and extracted at 70° C. for 3 hours to prepare a 40-45 Brix red ginseng extract.

(2) The red ginseng extract prepared in step (1) was ultra-filtered.

At this time, the membrane used in the ultrafiltration used at least one of cellulose acetate and polyethersulfone, the membrane pore size was 0.1 to 0.3 μm, the feed pressure was 5 to 20 bar, and it was carried out at room temperature. The ultrafiltration process was first filtered through a 50 μm sieve, then secondarily filtered through a 30 μm sieve, cooled to 20° C., filtered thirdly through a 20 μm sieve, and then cooled to 2° C. again, 0.1 A multi-stage filtration step of filtration through a sieve of μm was performed.

(3) The ultra-filtered red ginseng extract of step (2) was concentrated at 60-65° C. under a pressure of 600-670 mmHg for 3 hours to prepare a 65-70 Brix red ginseng concentrate.

Comparative Example 4. Red ginseng concentrate (glycerin fatty acid ester-cooking oil treatment)

(1) 500 mL of purified water and 1 g of glycerin fatty acid esters (Glycerin Esters of Fatty Acids, GEFA) were added to 100 g of red ginseng, and extracted at 70° C. for 3 hours to prepare a 40-45 Brix red ginseng extract.

(2) After adding canola oil to the red ginseng extract prepared in step (1) in a volume ratio of 9:1, it was stirred at 20° C. at 45 rpm for 30 to 60 minutes while circulating with a pump.

(3) The stirred red ginseng oil solution of step (2) was left at 20° C. for 24 hours, and the upper oil layer was removed from the red ginseng oil solution separated into an upper oil layer and a lower red ginseng extract layer.

(4) The red ginseng extract layer from which the oil layer of step (3) was removed was concentrated at 60-65° C. under a pressure of 600-670 mmHg for 3 hours to prepare a 65-70 Brix red ginseng concentrate.

Comparative Example 5. Red ginseng concentrate (glycerin fatty acid ester-ultrafiltration treatment)

(1) 500 mL of purified water and 1 g of glycerin fatty acid esters (Glycerin Esters of Fatty Acids, GEFA) were added to 100 g of red ginseng, and extracted at 70° C. for 3 hours to prepare a 40-45 Brix red ginseng extract.

(2) The red ginseng extract prepared in step (1) was ultra-filtered.

At this time, the membrane used in the ultrafiltration used at least one of cellulose acetate and polyethersulfone, the membrane pore size was 0.1 to 0.3 μm, the feed pressure was 5 to 20 bar, and it was carried out at room temperature. The ultrafiltration process was first filtered through a 50 μm sieve, then secondarily filtered through a 30 μm sieve, cooled to 20° C., filtered thirdly through a 20 μm sieve, and then cooled to 2° C. again, 0.1 A multi-stage filtration step of filtration through a sieve of μm was performed.

(3) The ultra-filtered red ginseng extract of step (2) was concentrated at 60-65° C. under a pressure of 600-670 mmHg for 3 hours to prepare a 65-70 Brix red ginseng concentrate.

Comparative Example 6. Red ginseng concentrate (edible oil-ultrafiltration treatment)

(1) 100 g of red ginseng was added with 500 mL of purified water and extracted at 70° C. for 3 hours to prepare a 40-45 Brix red ginseng extract.

(2) After adding canola oil to the red ginseng extract prepared in step (1) in a volume ratio of 9:1, it was stirred at 20° C. at 45 rpm for 30 to 60 minutes while circulating with a pump.

(3) The stirred red ginseng oil solution of step (2) was left at 20° C. for 24 hours, and the upper oil layer was removed from the red ginseng oil solution separated into an upper oil layer and a lower red ginseng extract layer.

(4) The red ginseng extract layer from which the oil layer of step (3) was removed was ultra-filtered.

At this time, the membrane used in the ultrafiltration used at least one of cellulose acetate and polyethersulfone, the membrane pore size was 0.1 to 0.3 μm, the feed pressure was 5 to 20 bar, and it was carried out at room temperature. The ultrafiltration process was first filtered through a 50 μm sieve, then secondarily filtered through a 30 μm sieve, cooled to 20° C., filtered thirdly through a 20 μm sieve, and then cooled to 2° C. again, 0.1 A multi-stage filtration step of filtration through a sieve of μm was performed.

(5) The ultra-filtered red ginseng extract of step (4) was concentrated at 60-65° C. under a pressure of 600-670 mmHg for 3 hours to prepare a 65-70 Brix red ginseng concentrate.

Experimental method

1. Analysis of pesticide residues by mass spectrometer

go. Analysis by LC-MS/MS (Liquid Chromatograph Mass Spectrometer)

5 g of the red ginseng concentrate prepared by each method was precisely weighed, put into a 50 mL centrifuge tube, 10 mL of acetonitrile containing an internal standard (0.1 mg/L triphenylphosphate) was added, and then shaken for 1-2 minutes.

Add 4.0(±0.2) g of anhydrous magnesium sulfate, 1.0(±0.05) g of sodium chloride, 1.0(±0.05) g of sodium citrate dihydrate, and 0.5(±0.03) g of disodium hydrogencitrate sesquihydrate to the solution in a centrifuge tube, and put it up and down for 1 minute. After mixing by shaking left and right, centrifugation (3,000 rpm/min, 5 minutes) was performed to separate the acetonitrile layer and the water layer, and then the acetonitrile layer was used as an LC-MS/MS sample extract.

Preparation of medium calibration calibration solution for LC-MS/MS (Liquid Chromatograph-Mass Spectrometer) division Standard material for calibration curve preparation sample solution One 2 3 4 target concentration 1 μg/L 5 μg/L 20 μg/L 50 μg/L preparation method buffer solution
600 μl +
acetonitrile
200 μl buffer solution
600 μl +
acetonitrile
200 μl buffer solution
600 μl +
acetonitrile
200 μl buffer solution
600 μl +
acetonitrile
200 μl Buffer 600 μl+
acetonitrile
300 μl STD
10 μg/L
×100 μl STD
50 μg/L
×100 μl STD
200 μg/L
×100 μl STD
500 μg/L
×100 μl Pesticide-free sample extract
100 μl Pesticide-free sample extract
100 μl Pesticide-free sample extract
100 μl Pesticide-free sample extract
100 μl sample extract
100 μl

Analysis conditions of LC-MS/MS (liquid chromatography-mass spectrometry) division Condition column C18 (2.1 mm × 150 mm, 2.6 ㎛) or equivalent mobile phase A mobile phase: aqueous solution containing 0.1% formic acid and 5 mM ammonium formate
B Mobile phase: Methanol solution containing 0.1% formic acid and 5 mM ammonium formate column temperature 35℃ injection volume 10 μl ionization method Electrospray ionization (ESI, positive)

Mobile Phase Program of LC-MS/MS (Liquid Chromatograph-Mass Spectrometer) hours (minutes) Flow rate (mL/min) Mobile phase A (%) Mobile phase B (%) 0 0.3 85 15 1.0 0.3 85 15 1.5 0.3 40 60 10.0 0.3 10 90 12.0 0.3 10 90 12.1 0.3 2 98 16.0 0.3 2 98 16.1 0.3 85 15 20.0 0.3 85 15

me. Analysis by GC-MS/MS (Gas Chromatograph Mass Spectrometer)

5 g of the red ginseng concentrate prepared by each method was precisely weighed, put into a 50 mL centrifuge tube, 10 mL of acetonitrile containing an internal standard (0.1 mg/L triphenylphosphate) was added, and then shaken for 1-2 minutes.

Add 4.0(±0.2) g of anhydrous magnesium sulfate, 1.0(±0.05) g of sodium chloride, 1.0(±0.05) g of sodium citrate dihydrate, and 0.5(±0.03) g of disodium hydrogencitrate sesquihydrate to the solution in a centrifuge tube, and put it up and down for 1 minute. After mixing by shaking left and right, centrifugation (3,000 rpm/min, 5 minutes) was performed to separate the acetonitrile layer and the water layer, and then the acetonitrile layer was separated. The acetonitrile layer separation solution was placed in a powder-phase extraction tube containing 25 mg of PSA and 150 mg of anhydrous magnesium sulfate, shaken for 1 minute, centrifuged (10,000 rpm/min, 2 minutes), and filtered through a membrane filter (0.2 μm). It was used as a GC-MS/MS sample extract.

Preparation of medium calibration calibration solution for GC-MS/MS (gas chromatograph-mass spectrometer) division Standard material for calibration curve preparation sample solution One 2 3 4 target concentration 10 μg/L 50 μg/L 200 μg/L 500 μg/L preparation method AP 20 μl AP 20 μl AP 20 μl AP 20 μl AP 20 μl internal standard material
1000 μg/L
×50 μl internal standard material
1000 μg/L
×50 μl internal standard material
1000 μg/L
×50 μl internal standard material
1000 μg/L
×50 μl acetonitrile
75 μl STD
200 μg/L
×25 μl STD
1,000 μg/L
×25 μl STD
4,000 μg/L
×25 μl STD
10,000 μg/L
×25 μl Pesticide-free sample extract
405 μl Pesticide-free sample extract
405 μl Pesticide-free sample extract
405 μl Pesticide-free sample extract
405 μl sample extract
405 μl

GC-MS/MS (gas chromatograph-mass spectrometer) measurement conditions division Condition column DB-1701 Capillary column (0.25 mm I.D. × 30 m, 0.25 ㎛) or equivalent column temperature Hold at 120°C for 2 minutes, increase the temperature by 10°C per minute to reach 280°C, and hold for 10 minutes. Adjust appropriately as needed. injection part temperature 240℃ injection method Split (10:1) detector Mass Spectrometer Selective Ion Mode Ionization method EI mode Ionization voltage 70 eV carrier gas Helium (flow rate: 1 mL per minute)

2. Phthalate analysis by GC-MS (Gas Chromatograph Mass Spectrometer)

Take 25 g of each red ginseng concentrate, transfer it to a separatory funnel, add 50 mL of a 1:1 (v/v) mixture of acetone and hexane, shake vigorously for 5 minutes, and then stand still, and transfer the acetone and hexane layers to a 250 mL flask. To the remaining filtrate, 50 mL of a mixed solution of acetone and hexane was added, and the same operation was repeated twice. The acetone and hexane layers were combined in the flask above and concentrated under reduced pressure. Then, the residue was dissolved in acetone to make 5 mL, and the solution was used as the test solution.

GC-MS (gas chromatograph-mass spectrometer) measurement conditions division Condition column DB-1701 capillary column (0.25 mm I.D. × 30 m, 0.25 μm) or equivalent. injection part Programmed Temperature Vaporizer (PTV) or equivalent
After injecting the sample solution at 80°C and holding it for 0.5 minutes, increase it to 280°C at the rate of 200°C/min and keep it for 29 minutes
The split ratio is set to 50:1 for 1.5 minutes after maintaining the initial condition 20:1, splitless for 0.5 minutes injection volume 3 μl Oven After injecting the sample solution at 90°C and holding it for 3 minutes, increase it to 120°C at the rate of 20°C/min, increase it to 300°C at the rate of 8°C/min, and hold it for 3 minutes mass spectrometer Multiple reaction monitoring (MRM) mode Electron multiplier voltage : 1,100 V
- scan time: about 1 second
- Mass peak width: quad1 (1.5 m/z), quad3 (2.5 m/z)
- Transfer line: 280℃, Manifold: 40℃, Ion source: 230℃

Example 1. Residual pesticide content before and after processing

The results of comparing the residual pesticide content of 320 kinds of red ginseng concentrate according to the process treatment are shown in Tables 7 to 17 below.

Residual pesticide content (ppm)-1 division pesticide ingredients Residual amount before treatment
(control group) Residual amount after three-step treatment (Preparation Example 1) Removal rate (%) One Abamectin B1 0.081 ND 100.0 2 Acephate 0.015 ND 100.0 3 Acetamiprid 0.069 ND 100.0 4 Acrinathrin 0.124 ND 100.0 5 Alachlor 0.226 ND 100.0 6 Aldicarb 0.032 ND 100.0 7 Aldrin 0.189 ND 100.0 8 Ametoctradin 0.050 ND 100.0 9 Amisulbrom 0.087 ND 100.0 10 Anilofos 0.386 ND 100.0 11 Azaconazole 0.051 ND 100.0 12 Azimsulfuron 0.081 ND 100.0 13 Azinphos-methyl 0.110 ND 100.0 14 Azoxystrobin 0.093 ND 100.0 15 Bendiocarb 0.097 ND 100.0 16 Benfursate 0.076 ND 100.0 17 Bensulfuron-methyl 0.074 ND 100.0 18 Benthiavalicarb-isopropyl 0.064 ND 100.0 19 Benzobicyclon 0.073 ND 100.0 20 Benzoximate 0.068 ND 100.0 21 BHC(alpha,beta,delta) 0.229 ND 100.0 22 Bifenox 0.127 ND 100.0 23 Bifenthrin 0.125 ND 100.0 24 Bitertanol 0.039 ND 100.0 25 Boscalid 0.119 ND 100.0 26 Bromacil 0.040 ND 100.0 27 Bromobutide 0.149 ND 100.0 28 Bromopropylate 0.245 ND 100.0 29 Buprofezin 0.053 ND 100.0 30 Butachlor 0.058 ND 100.0

Residual pesticide content (ppm)-2 division pesticide ingredients Residual amount before treatment
(control group) Residual amount after three-step treatment (Preparation Example 1) Removal rate (%) 31 Butafenacil 0.320 ND 100.0 32 Cadusafos 0.057 ND 100.0 33 Cafenstrole 0.059 ND 100.0 34 Carbaryl 0.050 ND 100.0 35 Carbendazim 0.299 ND 100.0 36 Carbofuran 0.047 ND 100.0 37 Carbophenothion 0.150 ND 100.0 38 carboxin 0.043 ND 100.0 39 Carfentrazone-ethyl 0.065 ND 100.0 40 Carpropamide 0.063 ND 100.0 41 Chlorantraniliprole 0.070 ND 100.0 42 Chlordane (2 isomer)-cis,trans 0.160 ND 100.0 43 Chlorfenapyr 0.080 ND 100.0 44 Chlorfenvinphos (2 isomer) E,Z 0.201 ND 100.0 45 Chlorfluazuron 0.058 ND 100.0 46 Chlorobenzilate 0.213 ND 100.0 47 Chlorpropham 0.121 ND 100.0 48 Chlorpyrifos 0.047 ND 100.0 49 Chlorpyrifos-methyl 0.085 ND 100.0 50 Chlorsulfuron 0.173 ND 100.0 51 Chromafenozide 0.042 ND 100.0 52 Clethodim 0.051 ND 100.0 53 Clofentezine 0.040 ND 100.0 54 Clomazone 0.061 ND 100.0 55 Clothianidin 0.073 ND 100.0 56 Cyazofamid 0.065 ND 100.0 57 Cyclosulfamuron 0.098 ND 100.0 58 Cyflufenamid 0.059 ND 100.0 59 Cyfluthrin (4 isomers) 1,2,3,4 0.698 ND 100.0 60 Cyhalofop-butyl 0.038 ND 100.0

Residual pesticide content (ppm)-3 division pesticide ingredients Residual amount before treatment
(control group) Residual amount after three-step treatment (Preparation Example 1) Removal rate (%) 61 Cyhalothrin1,2 0.197 ND 100.0 62 Cymoxanil 0.337 ND 100.0 63 Cypermethrin1,2,3,4 0.159 ND 100.0 64 Cyproconazole (l,II) 0.065 ND 100.0 65 Cyprodinil 0.055 ND 100.0 66 Daimuron 0.045 ND 100.0 67 Deltamethrin 1.041 ND 100.0 68 Demeton-S-Methyl 0.034 ND 100.0 69 Diazinon 0.034 ND 100.0 70 Dichlorvos (DDVP) 0.028 ND 100.0 71 Diclofop-methyl 0.104 ND 100.0 72 Dicloran 0.164 ND 100.0 73 Dicofol 0.073 ND 100.0 74 Dieldrin 0.090 ND 100.0 75 Diethofencarb 0.060 ND 100.0 76 Difenoconazole (2 isomer) 1,2 0.057 ND 100.0 77 Diflubenzuron 0.068 ND 100.0 78 Dimepiperate 0.048 ND 100.0 79 Dimethametryn 0.075 ND 100.0 80 Dimethenamid 0.053 ND 100.0 81 Dimethoate 0.049 ND 100.0 82 Dimethylvinphos E,Z 0.098 ND 100.0 83 Dimethylvinphos E,Z 0.058 ND 100.0 84 Diniconazole 0.044 ND 100.0 85 Dinotefuran 0.045 ND 100.0 86 Diphenamid 0.060 ND 100.0 87 Diphenylamine 0.079 ND 100.0 88 Disulfoton 0.124 ND 100.0 89 Dithiopyr 0.060 ND 100.0 90 Diuron 0.097 ND 100.0

Residual pesticide content (ppm)-4 division pesticide ingredients Residual amount before treatment
(control group) Residual amount after three-step treatment (Preparation Example 1) Removal rate (%) 91 Edifenphos 0.059 ND 100.0 92 Endosulfan (alpha, beta, sulfate) 0.213 ND 100.0 93 Endrin 0.140 ND 100.0 94 EPN 0.055 ND 100.0 95 Epoxiconazole 0.198 ND 100.0 96 Esprocarb 0.432 ND 100.0 97 Ethaboxam 0.044 ND 100.0 98 Ethhalfluralin 0.146 ND 100.0 99 Ethiofencarb 0.033 ND 100.0 100 Ethion 0.180 ND 100.0 101 Ethoprophos 0.060 ND 100.0 102 Ethoxysulfuron 0.347 ND 100.0 103 Etofenprox 0.035 ND 100.0 104 Etoxazole 0.039 ND 100.0 105 Etridiazole 0.100 ND 100.0 106 Etrimfos 0.034 ND 100.0 107 Famoxadone 0.087 ND 100.0 108 Fenamiphos 0.090 ND 100.0 109 Fenarimol 0.069 ND 100.0 110 Fenazaquin 0.052 ND 100.0 111 Fenbuconazole 0.050 ND 100.0 112 Fenclorim 0.055 ND 100.0 113 Fenhexamid 0.078 ND 100.0 114 Fenitrothion 0.157 ND 100.0 115 Fenobucarb 0.059 ND 100.0 116 Fenothiocarb 0.054 ND 100.0 117 Fenoxanil 0.078 ND 100.0 118 Fenoxaprop-ethyl 0.021 ND 100.0 119 Fenoxycarb 0.051 ND 100.0 120 Fenpropathrin 0.242 ND 100.0

Residual pesticide content (ppm)-5 division pesticide ingredients Residual amount before treatment
(control group) Residual amount after three-step treatment (Preparation Example 1) Removal rate (%) 121 Fenpyroximate 0.051 ND 100.0 122 Fenthion 0.041 ND 100.0 123 Fentrazamide 0.059 ND 100.0 124 Fenvalerate (2 isomer) 1,2 3.930 ND 100.0 125 Ferimzone (E,Z) 0.028 ND 100.0 126 Fipronil 0.056 ND 100.0 127 Flonicamid 0.070 ND 100.0 128 Fluacrypyrim 0.043 ND 100.0 129 Flubendiamide 0.064 ND 100.0 130 Flucetosulfuron 0.194 ND 100.0 131 Flucythrinate (2 isomer) 1,2 1.124 ND 100.0 132 Fludioxonil 0.222 ND 100.0 133 Flufenacet 0.067 ND 100.0 134 Flufenoxuron 0.057 ND 100.0 135 Flumioxazine 0.217 ND 100.0 136 Fluopicolide 0.079 ND 100.0 137 Fluopyram 0.061 ND 100.0 138 Fluquinconazole 0.060 ND 100.0 139 Flusilazole 0.048 ND 100.0 140 Flutolanil 0.088 ND 100.0 141 Fluxapyroxad 0.075 ND 100.0 142 Fonofos 0.081 ND 100.0 143 Forchlorfenuron 0.034 ND 100.0 144 Fosthiazate 0.030 ND 100.0 145 Fthalide 0.099 ND 100.0 146 Furathiocarb 0.055 ND 100.0 147 Gibberellic acid 0.178 ND 100.0 148 Halfenprox 0.235 ND 100.0 149 Halosulfuron-methyl 0.080 ND 100.0 150 haloxyfop 0.055 ND 100.0

Residual pesticide content (ppm)-6 division pesticide ingredients Residual amount before treatment
(control group) Residual amount after three-step treatment (Preparation Example 1) Removal rate (%) 151 Heptachlor 0.222 ND 100.0 152 Heptachlor-epoxide 0.097 ND 100.0 153 Hexaconazole 0.055 ND 100.0 154 Hexaflumuron 0.067 ND 100.0 155 Hexazinone 0.050 ND 100.0 156 Hexythiazox 0.056 ND 100.0 157 Imazalil 0.040 ND 100.0 158 Imazosulfuron 0.097 ND 100.0 159 Imibenconazole 0.124 ND 100.0 160 Imicyafos 0.052 ND 100.0 161 Imidacloprid 0.157 ND 100.0 162 Inabenfide 0.045 ND 100.0 163 Indanofan 0.278 ND 100.0 164 Indoxacarb 0.126 ND 100.0 165 Ifrobenfos 0.055 ND 100.0 166 Iprodione 0.048 ND 100.0 167 Iprovalicarb 0.054 ND 100.0 168 Isazofos 0.096 ND 100.0 169 Isofenphos 0.155 ND 100.0 170 Isoprocarb 0.052 ND 100.0 171 Isoprothiolane 0.057 ND 100.0 172 Isopyrazam 0.052 ND 100.0 173 Kresoxim-methyl 0.055 ND 100.0 174 Lindane (gamma-BHC) 0.077 ND 100.0 175 Linuron 0.057 ND 100.0 176 Lufenuron 0.074 ND 100.0 177 Malathion 0.055 ND 100.0 178 Mandipropamid 0.073 ND 100.0 179 Mecarbam 0.339 ND 100.0 180 Mefenacet 0.060 ND 100.0

Residual pesticide content (ppm)-7 division pesticide ingredients Residual amount before treatment
(control group) Residual amount after three-step treatment (Preparation Example 1) Removal rate (%) 181 Mepanipyrim 0.029 ND 100.0 182 Mepronil 0.059 ND 100.0 183 Metalaxyl 0.033 ND 100.0 184 metamifop 0.062 ND 100.0 185 Metazosulfuron 1.070 ND 100.0 186 Metconazole 0.060 ND 100.0 187 Methabenzthiazuron 0.045 ND 100.0 188 Methidathion 0.168 ND 100.0 189 Methiocarb 0.058 ND 100.0 190 Methomyl 0.041 ND 100.0 191 Methoxyfenozide 0.032 ND 100.0 192 Metobromuron 0.046 ND 100.0 193 Metolachlor 0.061 ND 100.0 194 Metolcarb 0.055 ND 100.0 195 Metrafenone 0.077 ND 100.0 196 Metribuzin 0.117 ND 100.0 197 Mevinphos 0.055 ND 100.0 198 Milbemectin A3, A4 0.100 ND 100.0 199 Molinate 0.053 ND 100.0 200 Monocrotophos 0.040 ND 100.0 201 Myclobutanil 0.090 ND 100.0 202 Napropamide 0.053 ND 100.0 203 Nicosulfuron 0.101 ND 100.0 204 Novaluron 0.025 ND 100.0 205 Nuarimol 0.055 ND 100.0 206 o,p-DDT,p,p-DDD,DDE,DDT 0.208 ND 100.0 207 Ofurace 0.058 ND 100.0 208 Omethoate 0.011 ND 100.0 209 Oxadiazon 0.067 ND 100.0 210 Oxadixyl 0.044 ND 100.0

Residual pesticide content (ppm)-8 division pesticide ingredients Residual amount before treatment
(control group) Residual amount after three-step treatment (Preparation Example 1) Removal rate (%) 211 Oxamyl 0.030 ND 100.0 212 Oxaziclomefone 0.053 ND 100.0 213 Oxyfluorfen 0.308 ND 100.0 214 Paclobutrazol 0.059 ND 100.0 215 Parathion-ethyl 0.210 ND 100.0 216 Parathion-methyl 0.158 ND 100.0 217 Penconazole 0.058 ND 100.0 218 Penencycuron 0.056 ND 100.0 219 Pendimethalin 0.066 ND 100.0 220 Penoxsulam 0.107 ND 100.0 221 Penthiopyrad 0.056 ND 100.0 222 Pentoxazone 0.072 ND 100.0 223 Permethrin (2 isomer) 0.171 ND 100.0 224 Phenthoate 0.117 ND 100.0 225 Phorate 0.204 ND 100.0 226 Phosalone 0.069 ND 100.0 227 Phosphamidon 0.009 ND 100.0 228 Phoxim 0.043 ND 100.0 229 Picoxystrobin 0.063 ND 100.0 230 Piperonyl butoxide 0.153 ND 100.0 231 Piperophos 0.062 ND 100.0 232 Pirimicarb 0.050 ND 100.0 233 Pirimiphos-ethyl 0.105 ND 100.0 234 pirimiphos-methyl 0.035 ND 100.0 235 Pretilachlor 0.133 ND 100.0 236 Probenazole 0.047 ND 100.0 237 Prochloraz 0.064 ND 100.0 238 Procymidone 0.089 ND 100.0 239 Profenofos 0.070 ND 100.0 240 Promecarb 1.550 ND 100.0

Residual pesticide content (ppm)-9 division pesticide ingredients Residual amount before treatment
(control group Residual amount after 3-step treatment Preparation Example 1) Removal rate (%) 241 Prometry 0.056 ND 100.0 242 Propachlor 0.127 ND 100.0 243 Propamocarb 0.016 ND 100.0 244 Propanil 0.078 ND 100.0 245 Propaquizafop 0.059 ND 100.0 246 Propazine 0.077 ND 100.0 247 Propiconazole (2 isomer)1,2 0.094 ND 100.0 248 propisochlor 0.102 ND 100.0 249 Propoxur 0.052 ND 100.0 250 Propyzamide 0.127 ND 100.0 251 Prothiofos 0.129 ND 100.0 252 Pyraclofos 0.086 ND 100.0 253 Pyraclostrobin 0.051 ND 100.0 254 Pyrazolate 0.040 ND 100.0 255 Pyrazophos 0.079 ND 100.0 256 Pyribenzoxim 0.052 ND 100.0 257 Pyributicarb 0.047 ND 100.0 258 Pyridaben 0.055 ND 100.0 259 Pyridalyl 0.238 ND 100.0 260 Pyridaphenthion 0.074 ND 100.0 261 Pyrifluquinazon 0.064 ND 100.0 262 Pyriftalid 0.064 ND 100.0 263 Pyrimethanil 0.058 ND 100.0 264 Pyrimidifen 0.072 ND 100.0 265 Pyriminobac-methyl(E,Z) 0.065 ND 100.0 266 Pyrimisulfan 0.070 ND 100.0 267 Pyriproxyfen 0.047 ND 100.0 268 Pyroquilon 0.059 ND 100.0 269 Quinalphos 0.058 ND 100.0 270 Quinmerac 0.040 ND 100.0

Residual pesticide content (ppm)-10 division pesticide ingredients Residual amount before treatment
(control group) Residual amount after three-step treatment (Preparation Example 1) Removal rate (%) 271 Quinoclamine 0.054 ND 100.0 272 Quintozene 0.200 ND 100.0 273 Quizalofop-ethyl 0.042 ND 100.0 274 Saflufenacil 0.115 ND 100.0 275 Sethoxydim 0.069 ND 100.0 276 Silafluofen 0.065 ND 100.0 277 Simazine 0.743 ND 100.0 278 Simeconazole 0.070 ND 100.0 279 Simetryn 0.046 ND 100.0 280 Spinetoram (J, L) 0.069 ND 100.0 281 Spirodiclofen 0.050 ND 100.0 282 Spiromesifen 0.039 ND 100.0 283 Spirotetramat 0.117 ND 100.0 284 Sulfoxaflor 0.060 ND 100.0 285 Tebuconazole 0.117 ND 100.0 286 Tebufenozide 0.068 ND 100.0 287 Tebufenpyrad 0.055 ND 100.0 288 Tebupirimfos 0.051 ND 100.0 289 Teflubenzuron 0.072 ND 100.0 290 Tefluthrin 0.082 ND 100.0 291 Terbufos 0.122 ND 100.0 292 Terbutylazine 0.042 ND 100.0 293 Terbutryn 0.053 ND 100.0 294 Tetraconazole 0.043 ND 100.0 295 Tetradifon 0.076 ND 100.0 296 Thenylchlor 0.067 ND 100.0 297 Thiabendazole 0.038 ND 100.0 298 Thiacloprid 0.060 ND 100.0 299 Thiamethoxam 0.083 ND 100.0 300 Thiazopyr 0.062 ND 100.0

Residual pesticide content (ppm)-11 division pesticide ingredients Residual amount before treatment
(control group) Residual amount after three-step treatment (Preparation Example 1) Removal rate (%) 301 Thidiazuron 0.049 ND 100.0 302 Thifensulfuron-methyl 0.072 ND 100.0 303 Thifluzamide 0.172 ND 100.0 304 Thiobencarb 0.046 ND 100.0 305 Thiodicarb 0.092 ND 100.0 306 Tiadinil 0.081 ND 100.0 307 Tolclofos-methyl 0.087 ND 100.0 308 tralomethrin 0.327 ND 100.0 309 Triadimefon 0.057 ND 100.0 310 Triadimenol 0.196 ND 100.0 311 Tri-allate 0.076 ND 100.0 312 Tricyclazole 0.039 ND 100.0 313 Trifloxystrobin 0.052 ND 100.0 314 Triflumizole 0.043 ND 100.0 315 Triflumuron 0.055 ND 100.0 316 Trifluralin 0.143 ND 100.0 317 Uniconazole 0.074 ND 100.0 318 Vamidothion 0.047 ND 100.0 319 Vinclozolin 0.089 ND 100.0 320 Zoxamide 0.073 ND 100.0

Example 2. Phthalate content before and after processing

The results of comparing the phthalate content of two types of red ginseng concentrate according to the process treatment are shown in Table 18 below. As a result, the process-treated red ginseng concentrate of Preparation Example 1 completely removed the following two types of phthalates.

Phthalate content (mg/kg) division phthalates Residual amount before treatment
(control group) Residual amount after three-step treatment (Preparation Example 1) Removal rate (%) One DBP 0.3642 ND 100 2 DEHP 1.1560 ND 100

Example 3. Residual pesticide content before and after processing

The results of comparing the residual pesticide content of 35 kinds of red ginseng concentrate according to the processing steps are shown in Tables 19 and 20 below. As a result, as in Preparation Example 1, the preparation of a red ginseng concentrate through glycerin fatty acid ester treatment, edible oil treatment, and ultrafiltration treatment was the most effective in reducing pesticide residues.

Reduction effect of pesticide residues by treatment process-1 division pesticide residue Residual amount of untreated group
(control group) 3 step processing
(Production Example 1) Step 1 single processing
(Comparative Example 1) 2nd stage single processing
(Comparative Example 2) 3 step single processing
(Comparative Example 3) residual amount
(ppm) Removal rate (%) Removal rate (%) Removal rate (%) Removal rate (%) One Acetamiprid 0.069 ND 100.0 62.3 92.0 10.8 2 Aldicarb 0.032 ND 100.0 56.8 62.2 1.7 3 Azoxystrobin 0.093 ND 100.0 75.9 95.2 47.1 4 Bifenthrin 0.125 ND 100.0 54.9 85.4 2.4 5 Bromacil 0.040 ND 100.0 54.9 94.2 5.9 6 Cadusafos 0.057 ND 100.0 46.9 44.9 11.6 7 Carbendazim 0.299 ND 100.0 34.8 95.7 8.7 8 Carbofuran 0.047 ND 100.0 84.2 93.2 29.8 10 Deltamethrin 1.041 ND 100.0 34.9 100.0 90.0 11 Diethofencarb 0.060 ND 100.0 54.8 100.0 2.4 12 Difenoconazole 0.057 ND 100.0 41.6 67.8 9.2 13 Dimethenamid 0.053 ND 100.0 76.4 100.0 0.0 14 Fenhexamid 0.078 ND 100.0 46.8 100.0 0.0 15 Fludioxonil 0.088 ND 100.0 56.7 67.4 19.6 16 Fluopicolide 0.079 ND 100.0 34.69 96.5 4.5 17 Flutolanil 0.088 ND 100.0 13.7 91.7 23.0 18 Hexaconazole 0.055 ND 100.0 16.9 70.6 16.3 19 Kresoxim-methyl 0.055 ND 100.0 43.6 82.9 18.8 20 Metalaxyl 0.033 ND 100.0 26.7 46.4 0.7 21 Penencycuron 0.056 ND 100.0 33.7 94.7 -3.1 22 Permethrin 0.171 ND 100.0 62.7 95.9 14.1 23 Procymidone 0.089 ND 100.0 42.6 84.8 6.9 24 Pyraclostrobin 0.051 ND 100.0 45.3 93.3 35.3 25 Pyrimethanil 0.058 ND 100.0 66.7 92.0 9.7 26 Tebuconazole 0.117 ND 100.0 12.4 91.9 3.8 27 Tebupirimfos 0.051 ND 100.0 23.6 97.2 19.0 28 Tefluthrin 0.082 ND 100.0 46.7 96.5 30.9 29 Terbufos 0.122 ND 100.0 51.7 40.3 23.9 30 Thiamethoxam 0.083 ND 100.0 46.7 80.2 48.8 31 Thiazopyr 0.062 ND 100.0 25.7 100.0 55.5 32 Thiodicarb 0.046 ND 100.0 46.5 63.4 16.2 33 Thifensulfuron-methyl 0.072 ND 100.0 33.4 98.7 20.0 34 Trifloxystrobin 0.052 ND 100.0 12.7 62.8 22.1 35 Triflumizole 0.043 ND 100.0 36.8 96.4 3.6

Reduction effect of pesticide residues by treatment process-2 pesticide residue Residual amount of untreated group
(control group) 3 step processing
(Production Example 1) Stage 1 processing, then stage 2 processing
(Comparative Example 4) Stage 1 processing, then stage 3 processing
(Comparative Example 5) Step 2 processing, then step 3 processing
(Comparative Example 6) residual amount
(ppm) Removal rate (%) Removal rate (%) Removal rate (%) Removal rate (%) One Acetamiprid 0.069 ND 100.0 68.1 34.3 92.0 2 Aldicarb 0.032 ND 100.0 76.1 17.0 63.1 3 Azoxystrobin 0.093 ND 100.0 100.0 100.0 100.0 4 Bifenthrin 0.125 ND 100.0 100.0 100.0 100.0 5 Bromacil 0.040 ND 100.0 100.0 59.1 100.0 6 Cadusafos 0.057 ND 100.0 75.4 71.0 58.0 7 Carbendazim 0.299 ND 100.0 56.5 56.5 56.5 8 Carbofuran 0.047 ND 100.0 100.0 68.6 100.0 10 Deltamethrin 1.041 ND 100.0 100.0 100.0 100.0 11 Diethofencarb 0.060 ND 100.0 100.0 75.9 100.0 12 Difenoconazole 0.057 ND 100.0 100.0 94.0 71.7 13 Dimethenamid 0.053 ND 100.0 100.0 100.0 100.0 14 Fenhexamid 0.078 ND 100.0 100.0 100.0 100.0 15 Fludioxonil 0.088 ND 100.0 87.0 41.3 63.0 16 Fluopicolide 0.079 ND 100.0 100.0 76.6 95.0 17 Flutolanil 0.088 ND 100.0 100.0 100.0 93.4 18 Hexaconazole 0.055 ND 100.0 76.3 78.7 73.6 19 Kresoxim-methyl 0.055 ND 100.0 100.0 60.8 87.1 20 Metalaxyl 0.033 ND 100.0 65.6 59.6 65.6 21 Penencycuron 0.056 ND 100.0 100.0 88.5 97.3 22 Permethrin 0.171 ND 100.0 100.0 67.6 95.9 23 Procymidone 0.089 ND 100.0 100.0 54.1 88.5 24 Pyraclostrobin 0.051 ND 100.0 100.0 99.3 93.3 25 Pyrimethanil 0.058 ND 100.0 90.2 96.1 94.6 26 Tebuconazole 0.117 ND 100.0 100.0 77.1 92.8 27 Tebupirimfos 0.051 ND 100.0 100.0 87.4 98.6 28 Tefluthrin 0.082 ND 100.0 93.0 51.9 96.5 29 Terbufos 0.122 ND 100.0 100.0 100.0 49.3 30 Thiamethoxam 0.083 ND 100.0 100.0 100.0 84.3 31 Thiazopyr 0.062 ND 100.0 100.0 100.0 100.0 32 Thiodicarb 0.046 ND 100.0 63.4 87.2 69.4 33 Thifensulfuron-methyl 0.072 ND 100.0 100.0 100.0 84.6 34 Trifloxystrobin 0.052 ND 100.0 76.2 98.8 64.4 35 Triflumizole 0.043 ND 100.0 64.3 64.3 64.3

Example 8. Sensory evaluation of edible oil-treated red ginseng extract layer

When red ginseng extract was treated with edible oil, 40 adults were ingested with a layer of red ginseng extract prepared by different types of edible oil. Preference was evaluated to be closer to 5 points as it was closer to the control group, and taste and odor were evaluated as 1 point when very bad and 5 points when very good.

Sensory evaluation of red ginseng extract layer according to types of cooking oil type of cooking oil untreated group canola oil soybean oil corn oil Canola Oil + Coconut Oil Canola Oil + Coconut Oil + Date Palm Oil Residual pesticide removal rate - over 90 over 90 over 90 over 90 over 90 Phthalate Removal Rate - over 90 over 90 over 90 over 90 over 90 sensuality preference 5.0 4.5 3.9 4.1 4.6 4.8 already 4.1 3.9 3.1 3.0 4.2 4.6 off-flavor 4.2 3.8 3.3 3.5 4.3 4.7

As a result, more than 90% of pesticides and phthalates were removed from all cooking oils, so there was no difference in the removal rate.

In addition, it was confirmed that the addition of both canola oil + coconut oil + date palm oil improved the preference compared to the untreated group in the rice and odor.

Example 9: Sensory evaluation of red ginseng concentrate

After ingesting 40 adults with each processed red ginseng concentrate, the higher the preference, the closer to 5 points, and the value divided by the average is shown in Table 22 below.

Sensory evaluation of red ginseng concentrate division Types of red ginseng concentrate control 4.1 Preparation Example 1 4.4 Preparation 2 4.7 Comparative Example 1 3.9 Comparative Example 2 3.9 Comparative Example 3 4.1 Comparative Example 4 4.0 Comparative Example 5 4.1 Comparative Example 6 4.1

As a result, the red ginseng concentrate having a more improved sensory compared to the untreated group (control group) was shown in Preparation Examples 1 and 2.

Claims (5)

(1) preparing a 40-45 Brix ginseng extract by adding 450-550 mL of water and 0.8-1.2 g of glycerin fatty acid ester to 80-120 g of ginseng and extracting it at 60-80°C for 2-4 hours;
(2) After adding canola oil, coconut oil and date palm oil to the ginseng extract prepared in step (1) in a volume ratio of 8~9:0.8~1.2:0.2~0.4:0.1~0.3, 30~ at 30~35℃ stirring for 60 minutes;
(3) The stirred ginseng oil solution of step (2) is left at 15-25° C. for 20-28 hours, and the upper oil layer is separated from the ginseng oil solution separated into the upper oil layer and the lower ginseng extract layer. removing;
(4) ultra-filtering the ginseng extract layer from which the oil layer of step (3) has been removed; and
(5) of the ginseng concentrate with reduced pesticide content, characterized in that it comprises the step of concentrating the ultra-filtered ginseng extract of the step (4) to 65-70 Brix at 60-65 ° C. for 2-4 hours manufacturing method. According to claim 1, wherein the ginseng is fresh ginseng, red ginseng, dried ginseng, white ginseng, black ginseng, Taegeuk ginseng, gok ginseng, wild ginseng, camphor ginseng, wild ginseng cultured root, sprout ginseng, and wild ginseng, characterized in that at least one ginseng selected from the group consisting of pesticide content A method for producing this reduced ginseng concentrate. delete delete A ginseng concentrate with reduced pesticide content prepared by the method of claim 1 or 2.