KR102375945B1 - Method for production of beer using rice and the rice beer thereof - Google Patents

Method for production of beer using rice and the rice beer thereof Download PDF

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KR102375945B1
KR102375945B1 KR1020200004531A KR20200004531A KR102375945B1 KR 102375945 B1 KR102375945 B1 KR 102375945B1 KR 1020200004531 A KR1020200004531 A KR 1020200004531A KR 20200004531 A KR20200004531 A KR 20200004531A KR 102375945 B1 KR102375945 B1 KR 102375945B1
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정찬희
정성채
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    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
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Abstract

본 발명에 따른 쌀을 이용한 맥주 제조방법은 맥아 60~70중량%와 고시히까리 품종 생벼 30~40중량%을 혼합하고, 50℃의 물에 침지시켜 당화효소를 용출시키는 용출단계; 상기 용출단계 이후, 65℃에서 호화 및 당화 반응으로 당화시키는 당화단계; 상기 당화단계 이후, 당화효소를 불활성화시키고 여과하는 여과단계; 상기 여과단계 이후, 여과 회수된 당화액에 호프를 첨가하고 자비시키는 자비단계; 및 상기 자비단계 이후, 효모를 첨가하고 발효시키는 발효단계를 포함하는 것을 특징으로 한다.The method for producing beer using rice according to the present invention comprises: an elution step of mixing 60 to 70% by weight of malt and 30 to 40% by weight of raw Koshihikari rice, and immersing it in water at 50° C. to elute a saccharifying enzyme; After the elution step, a saccharification step of saccharification by gelatinization and saccharification at 65°C; After the saccharification step, a filtration step of inactivating the saccharification enzyme and filtering; After the filtration step, boiling step of adding hops to the saccharification solution recovered by filtration and boiling; And after the boiling step, it is characterized in that it comprises a fermentation step of adding yeast and fermenting.

Figure 112020003834161-pat00001
Figure 112020003834161-pat00001

Description

쌀을 이용한 맥주 제조방법 및 그 제조방법에 의한 맥주{Method for production of beer using rice and the rice beer thereof}A method for manufacturing beer using rice and beer by the manufacturing method {Method for production of beer using rice and the rice beer thereof}

본 발명은 풍미가 우수한 쌀을 이용한 맥주 제조방법 및 그 제조방법에 의한 맥주에 관한 것으로, 보다 구체적으로는 일반쌀보다 영양성분이 매우 우수한 고시히까리 품종의 생벼쌀을 이용하여 드라이하면서도 풍미가 우수한 쌀을 이용한 맥주 제조방법 및 그 제조방법에 의한 맥주에 관한 것이다.The present invention relates to a method for manufacturing beer using rice with excellent flavor and beer by the manufacturing method, and more specifically, to rice with excellent flavor while being dried using raw rice of Koshihikari, which has a very superior nutritional component than regular rice. It relates to a method for producing beer using the method and to beer by the method for producing the same.

맥주는 현재 국내의 젊은층에서 널리 음용되고 있는 서양의 발효주로, 통상적으로, 보리 또는 밀을 발아시키고 발아 시 생성된 당화 효소를 비롯한 각종 효소에 의해 보리 또는 밀 속의 전분을 당화시켜 당분으로 전환시킨 다음 호프를 첨가하고 자비시켜 당화액을 제조한 후, 맥주효모를 첨가하여 알코올을 생성시키며, 숙성 중에 탄산가스를 잔류시키는 단행복 발효방식에 의해 제조하고 있다.Beer is a Western fermented liquor widely consumed by young people in Korea. Usually, barley or wheat is germinated and the starch in barley or wheat is saccharified by various enzymes including saccharification enzymes produced during germination to convert it into sugar. After adding hops and boiling to prepare a saccharified liquid, brewer's yeast is added to produce alcohol, and carbon dioxide is left behind during ripening.

특히, 맥주는 알코올 함량 약 4% 정도인 황금색의 투명한 액체로 탄산가스를 함유하고 있으며, 백색 크림형의 거품을 나타내는 특징이 있다. 이러한 맥주는 세계 여러 나라의 각 지방마다 그 맛이 다르며, 알코올을 함유한 대중음료로 자리 잡고 있다. 최근 다양한 특징을 가진 맥주의 수입 증대 등 주류 사이클의 변화로 국산 쌀 소비를 이끌어갈 주류 제품 개발 필요성이 제기되고 있으며, 새로운 풍미와 맛을 제공할 수 있는 쌀을 이용한 맥주의 제조 방법에 대한 연구가 요구되고 있다. 이와 관련하여서는 선행기술문헌으로서는 대한민국 등록특허공보 등록번호 제10-1002740호 '쌀맥주의 제조 방법 및 그로부터 제조된 쌀맥주'가 있다.In particular, beer is a golden, transparent liquid with an alcohol content of about 4%, containing carbon dioxide, and exhibiting white creamy foam. The taste of these beers is different in each region of various countries around the world, and it is established as a popular drink containing alcohol. Recently, the need to develop alcoholic beverage products that will lead domestic rice consumption has been raised due to changes in the liquor cycle, such as an increase in imports of beer with various characteristics, and research on a method for manufacturing beer using rice that can provide new flavor and taste is being demanded In this regard, as a prior art document, there is Republic of Korea Patent Publication No. 10-1002740 'Method for producing rice beer and rice beer prepared therefrom'.

본 발명은 새로운 풍미와 크리스피한 맛을 제공하며 드라이한 스타일의 고시히카리 품종 생벼 쌀을 이용한 고급 품질의 맥주를 제공하는데 그 목적이 있다.An object of the present invention is to provide a high-quality beer using dry style Koshihikari cultivar raw rice, which provides a new flavor and crispy taste.

본 발명은 맥아 60~70중량%와 고시히까리 품종 생벼 30~40중량%을 혼합하고, 50℃의 물에 침지시켜 당화효소를 용출시키는 용출단계; 상기 용출단계 이후, 65℃에서 호화 및 당화 반응으로 당화시키는 당화단계; 상기 당화단계 이후, 당화효소를 불활성화시키고 여과하는 여과단계; 상기 여과단계 이후, 여과 회수된 당화액에 호프를 첨가하고 자비시키는 자비단계; 및 상기 자비단계 이후, 효모를 첨가하고 발효시키는 발효단계를 포함하는 것을 특징으로 하는 쌀을 이용한 맥주 제조방법을 제공할 수 있다.The present invention is an elution step of mixing 60 to 70% by weight of malt and 30 to 40% by weight of raw rice of the Koshihikari variety, and immersing it in water at 50° C. to elute the saccharifying enzyme; After the elution step, a saccharification step of saccharification by gelatinization and saccharification at 65°C; After the saccharification step, a filtration step of inactivating the saccharification enzyme and filtering; After the filtration step, boiling step of adding hops to the saccharification solution recovered by filtration and boiling; And after the boiling step, it can provide a method for producing beer using rice, characterized in that it comprises a fermentation step of adding yeast and fermenting.

한편, 본 발명의 상기 발효단계에서, 15℃에서 발효시켜 알코올 함량이 4%가 되도록 발효를 시키는 1차 발효 단계; 및 저온인 5℃에서 발효시켜 알코올 함량이 5%가 되도록 발효시키는 2차 발효단계를 특징으로 하는 쌀을 이용한 맥주 제조방법을 제공할 수 있다.On the other hand, in the fermentation step of the present invention, a primary fermentation step of fermenting at 15 ℃ so that the alcohol content is 4%; And it is possible to provide a beer manufacturing method using rice, characterized in that the secondary fermentation step of fermentation at a low temperature of 5 ℃ fermentation so that the alcohol content is 5%.

한편, 본 발명의 상기 용출단계에서, 상기 맥아와 고시히까리 품종벼로 조성된 홉합곡물량 대비 500~600중량%의 물에 침지시키는 것을 특징으로 하는 쌀을 이용한 맥주 제조방법을 제공할 수 있다.On the other hand, in the dissolution step of the present invention, it is possible to provide a method for producing beer using rice, characterized in that it is immersed in water in an amount of 500 to 600% by weight compared to the amount of hops made from the malt and Koshihikari rice variety.

한편, 본 발명의 상기 용출단계에서, 15 ~ 20분 동안 침지시키는 것을 특징으로 하는 쌀을 이용한 맥주 제조방법을 제공할 수 있다.On the other hand, in the elution step of the present invention, it is possible to provide a beer manufacturing method using rice, characterized in that the immersion for 15 to 20 minutes.

한편, 본 발명의 상기 당화단계에서, 100 ~ 150분 동안 호화 및 당화 반응을 진행하는 것을 특징으로 하는 쌀을 이용한 맥주 제조방법을 제공할 수 있다.On the other hand, in the saccharification step of the present invention, it is possible to provide a beer manufacturing method using rice, characterized in that the gelatinization and saccharification reaction proceeds for 100 to 150 minutes.

또한, 본 발명은 쌀을 이용한 맥주 제조방법에 의하여 제조되는 맥주를 제공할 수 있다.In addition, the present invention can provide beer produced by a method for producing beer using rice.

본 발명에 따른 쌀맥주 제조방법은 일반쌀보다 영양성분이 매우 우수한 고시히카리 품종 생벼 쌀을 이용하여, 새로운 풍미와 크리스피한 맛을 제공하며 드라이한 스타일의 고시히카리 품종 쌀을 이용한 고급 품질의 맥주를 제조할 수 있다.The rice beer manufacturing method according to the present invention uses raw Koshihikari rice, which has a very superior nutritional component than regular rice, to provide a new flavor and crispy taste, and to produce high-quality beer using dry style Koshihikari rice. can

도1은 본 발명의 실시예에 따른 쌀을 이용한 맥주 제조방법의 순서를 나타낸 도면이다.1 is a view showing the sequence of the beer manufacturing method using rice according to an embodiment of the present invention.

이하 본 발명에 따른 쌀을 이용한 맥주 제조방법 및 그 제조방법에 의한 맥주를 구체적으로 설명하기로 한다.Hereinafter, a method for manufacturing beer using rice according to the present invention and beer by the manufacturing method will be described in detail.

도1은 본 발명의 실시예에 따른 쌀을 이용한 맥주 제조방법의 순서를 나타낸 도면이다.1 is a view showing the sequence of the beer manufacturing method using rice according to an embodiment of the present invention.

도1을 참조하면, 본 발명의 실시예에 따른 쌀을 이용한 맥주 제조방법은 맥아와 고시히까리 품종 생벼 쌀을 혼합하고 50℃의 물에 침지시켜 당화효소를 용출시키는 용출단계(S100), 상기 용출단계 이후, 65℃에서 호화 및 당화 반응으로 당화시키는 당화단계(S200), 상기 당화단계 이후, 당화효소를 불활성화시키고 여과하는 여과단계(S300), 상기 여과단계 이후, 여과 회수된 당화액에 호프를 첨가하고 자비시키는 자비단계(S400); 및 상기 자비단계 이후, 효모를 첨가하고 발효시키는 발효단계(S500)를 포함할 수 있다.Referring to Figure 1, the beer manufacturing method using rice according to an embodiment of the present invention is an elution step (S100) of mixing malt and raw rice of Koshihikari variety and immersing it in water at 50 ° C to elute the saccharifying enzyme (S100), the elution step Thereafter, a saccharification step of saccharification by gelatinization and saccharification at 65° C. (S200), a filtration step of inactivating and filtering a saccharification enzyme after the saccharification step (S300), and after the filtration step, hops are added to the filtered and recovered saccharification solution. A boiling step of adding and boiling (S400); And after the boiling step, it may include a fermentation step (S500) of adding yeast and fermenting.

상기 맥아와 고시히까리 품종 생벼 쌀을 혼합하고 50℃의 물에 침지시켜 당화효소를 용출시키는 용출단계(S100)는 고시히까리 생벼 쌀과 맥아로 혼합한 후, 50℃의 물에 침지시켜 당화효소를 용출시키는 단계이다. 상기 맥아는 60~70중량%이며, 상기 고시히까리 생벼 쌀 30~40중량%인 것이 가장 바람직한데, 상기 고시히까리 생벼 쌀이 30중량% 미만으로 첨가되는 경우에는 맥주에서 특유한 새로운 풍미가 감소되어 좋지 않고, 40중량%를 초과하는 경우에는 맥아의 첨가량이 상대적으로 감소하므로 맥주가 가지는 특유의 풍미와 제품 특성이 충분히 발휘되지 못하기 때문에 바람직하지 않다.The elution step (S100) of mixing the malt and Koshihikari raw rice rice and immersing it in water at 50° C. to elute the saccharification enzyme is a step (S100) of mixing Koshihikari raw rice and malt, followed by immersion in water at 50° C. to elute the saccharification enzyme. is a step The malt is 60 to 70% by weight, and it is most preferably 30 to 40% by weight of the raw Koshihikari rice. When it exceeds 40% by weight, the amount of malt added is relatively reduced, and thus the unique flavor and product characteristics of beer are not sufficiently exhibited.

상기 맥아와 고시히까리 생벼 쌀을 혼합하여 50℃ 물에 침지시켜 당화효소를 용출시키는데, 효소의 용출이 효율적이면서 단백질 분해효소의 활성이 증가하여 고소한 맛을 내는 아미노산을 생성할 수 있기 때문이다. 여기서, 물의 함량은 특정의 범위에 한정되는 것은 아니나, 바람직하게는 생벼와 맥아로 조성된 혼합 곡물 양 대비 500~600중량%인 것이 바람직하다. 한편, 침지하는 시간은 특정의 범위에 한정되는 것은 아니나, 바람직하게는 15분인 것이 좋은데, 맥아와 고시히까리 생벼 쌀로부터 당화효소를 충분히 경제적으로 용출시킬 수 있기 때문이다.The malt and raw Koshihikari rice are mixed and immersed in water at 50° C. to elute the saccharification enzyme. This is because the enzyme elution is efficient and the activity of the proteolytic enzyme increases to produce an amino acid with a fragrant taste. Here, the content of water is not limited to a specific range, but is preferably 500 to 600% by weight relative to the amount of mixed grains composed of raw rice and malt. On the other hand, the immersion time is not limited to a specific range, but preferably 15 minutes, because the saccharification enzyme can be sufficiently economically eluted from malt and raw Koshihikari rice.

일반적으로 맥아는 겉보리에 수분·온도·산소를 작용시켜 발아시킨 보리의 낟알로, 녹말과 같은 당류(糖類), 비타민류와 여러 효소를 함유하고 있으며, 맥주에 맥아 고유의 풍미를 제공한다. 맥아는 당화에 필요한 효소 및 그밖에 여러 가지 효소들을 용출시켜 단백질을 아미노산으로 분해하고, 생벼의 전분을 쌀겨의 당화 효소와 함께 발효성당으로 분해한다. In general, malt is a grain of barley germinated by applying moisture, temperature, and oxygen to the outer barley, and contains sugars such as starch, vitamins, and various enzymes, and provides the unique flavor of malt to beer. Malt elutes enzymes and other enzymes necessary for saccharification to break down proteins into amino acids, and raw rice starch into fermented sugars together with saccharification enzymes from rice bran.

한편, 고시히까리 쌀 품종은 조생종 숙기, 현미천립중 21g, 아밀로스 함량 18.6%, 단백질 함량 6%하고 있는 가지며 일반적인 쌀과 비교하여 우수한 영양성분을 가지고 있는 품종이며, 다소 경장난이도가 높은 것으로, 대한민국에서는 경기도 김포에서 주로 재배되고 있다. 통상적으로 쌀은 쌀눈에 약 66%, 쌀겨에 약 29%, 백미에 약 5% 영양소가 분포되어 있기 때문에 도정된 벼 즉, 백미보다 영양소가 풍부하고, 쌀눈을 포함하는 쌀겨는 탄수화물 약 45%, 식이섬유 약 25%, 지방 약 16%, 단백질 약 14%와 미량의 비타민, 칼슘, 마그네슘, 인 등의 미네랄을 함유하며 당화에 필요한 효소를 다량으로 함유하고 있다. 본 발명은 고시히까리 품종의 생벼 쌀을 맥아와 홉합하여 사용하여, 고시히까리 품종 생벼 쌀을 첨가하여, 쌀겨에 함유된 당화 효소는 맥아 유래의 당화효소와 더불어 전분의 호화 및 당화를 수행하기 때문에, 맥아만을 사용하여 당화공정을 수행하는 일반 맥주의 제조방법과 비교하여 비교적 낮은 온도에서도 당화를 수행할 수 있고, 당화를 촉진하기 위해 외부로부터 별도로 당화효소를 첨가시켜 줄 필요가 없다. 또한, 생벼를 사용하기 때문에 백미에는 함유되지 않은 성분들이 맥주의 향기 성분을 증가시켜 풍미가 우수한 맥주를 제조할 수 있다.On the other hand, the Koshihikari rice variety is a variety with excellent nutritional content compared to ordinary rice, with 21 g of brown rice, 18.6% of amylose, and 6% of protein, and has a somewhat higher difficulty of play. It is mainly cultivated in In general, rice contains about 66% of the nutrients in the rice germ, about 29% in the bran, and about 5% in the polished rice, so it has more nutrients than polished rice, that is, white rice. It contains about 25% of dietary fiber, about 16% of fat, about 14% of protein, trace vitamins, minerals such as calcium, magnesium, and phosphorus, and contains a large amount of enzymes necessary for saccharification. In the present invention, raw rice of Koshihikari variety is mixed with malt, raw rice of Koshihikari variety is added, and the saccharification enzyme contained in rice bran performs gelatinization and saccharification of starch together with malt-derived saccharification enzyme. Saccharification can be performed even at a relatively low temperature compared to the manufacturing method of general beer in which a saccharification process is performed using a saccharification process, and there is no need to separately add a saccharification enzyme from the outside to promote saccharification. In addition, since raw rice is used, ingredients not contained in white rice increase the aroma component of the beer, thereby making beer with excellent flavor.

상기 용출단계 이후, 65℃에서 호화 및 당화 반응으로 당화시키는 당화단계(S200)는 침지 후, 62~68℃에서 호화 및 당화시키는 단계이다. 여기서, 입자가 치밀한 전분에 물을 가하고 온도를 높여 팽윤시킴으로써 입자 간의 공간이 생기고 투명한 형태의 콜로이드 상태가 되는 호화과정을 거치는데, 호화된 전분은 생전분보다 효소의 침투가 용이하기 때문에 전분의 이용률이 증가하여 발효성당으로 쉽게 전환될 수 있다. 또한, 당화 과정을 통해 전분이 발효성당으로 변화하는데, 발효성당이란 효모가 대사를 위해 이용 가능한 당으로, 효모는 발효성당을 섭취하여 맥주에 필요한 알코올과 이산화탄소를 생성한다.After the elution step, the saccharification step (S200) of saccharification by gelatinization and saccharification at 65° C. is a step of gelatinization and saccharification at 62 to 68° C. after immersion. Here, by adding water to starch with dense particles and increasing the temperature to swell, spaces between particles are created and the gelatinization process becomes a transparent colloidal state. The utilization rate of starch is because gelatinized starch is easier to penetrate than raw starch. This increase can be easily converted into fermented sugar. In addition, starch is changed to fermented sugar through saccharification process. Fermented sugar is a sugar that yeast can use for metabolism, and yeast consumes fermented sugar to produce alcohol and carbon dioxide necessary for beer.

한편, 본 발명은 기존의 맥주 제조 방법에서 사용하는 70℃ 정도의 호화 온도에 비해 상대적으로 낮은 온도에서 호화 및 당화 공정을 수행하는데, 당화를 위한 효소가 맥아뿐만 아니라 고시히까리 생벼 쌀로부터 유래되어 충분히 존재하기 때문이다. 또한, 통상적으로는 호화 후 온도를 낮추는 별도 단계에 의해 당화공정을 수행하는데, 본 발명은 통상적인 호화 온도보다 상대적으로 낮은 온도인 62~68℃에서 호화를 시키기 때문에 별도의 당화 공정을 추가로 설계하지 않고, 바로 해당 온도에서 당화를 동시에 수행할 수 있다. 또한, 낮은 온도에서 호화를 수행하기 때문에, 향기성분들의 휘발 정도를 감소시켜, 풍미 성분들을 상대적으로 많이 포집할 수도 있다. 한편, 호화 및 당화를 수행하는 시간은 특정의 범위에 한정되는 것은 아니나, 바람직하게는 100~140분 동안 수행하는 것이 좋은데, 호화 및 당화가 충분히 경제적으로 수행되기 때문이다. On the other hand, the present invention performs the gelatinization and saccharification process at a relatively low temperature compared to the gelatinization temperature of about 70°C used in the existing beer manufacturing method, and the enzyme for saccharification is derived from malt as well as raw Koshihikari rice and is sufficiently present. because it does In addition, in general, the saccharification process is performed by a separate step of lowering the temperature after gelatinization, but in the present invention, a separate saccharification process is additionally designed because gelatinization is performed at 62-68° C., which is a relatively lower temperature than the conventional gelatinization temperature. Without this, saccharification can be performed at the same temperature at the same time. In addition, since the gelatinization is performed at a low temperature, it is possible to reduce the degree of volatilization of the fragrance components, thereby capturing a relatively large number of flavor components. On the other hand, the time for performing gelatinization and saccharification is not limited to a specific range, but preferably for 100 to 140 minutes, since gelatinization and saccharification are sufficiently economically performed.

상기 당화단계 이후, 당화효소를 불활성화시키고 여과하는 여과단계(S300)은호화 및 당화 반응 후, 당화효소를 불활성화시키고 여과하는 단계이다. 당화 효소의 불활성화는 특정 조건에서 반드시 수행되어야 하는 것은 아니나, 바람직하게 일 예로 72~78℃에서 10~20분 동안 수행하는 것이 좋다. After the saccharification step, the filtration step (S300) of inactivating and filtering the saccharifying enzyme is a step of inactivating and filtering the saccharifying enzyme after gelatinization and saccharification. The inactivation of the saccharification enzyme is not necessarily performed under specific conditions, but is preferably performed, for example, at 72 to 78° C. for 10 to 20 minutes.

한편, 본 발명은 여과를 통해 생벼 및 맥아의 껍질이 제거되어 맥주 제조시 불순물의 침전을 예방할 수 있다. 이때 여과는 특정 방법에 반드시 한정되는 것은 아니나, 바람직하게는 당화 효소를 불활성화시킨 당화액을 여과조에 옮긴 후, 정치함으로써 여과하는데, 맑은 여과액이 나올 때까지 반복 수행하는 것이 좋다.Meanwhile, in the present invention, the husks of raw rice and malt are removed through filtration to prevent precipitation of impurities during beer production. At this time, the filtration is not necessarily limited to a specific method, but preferably, the saccharification solution in which the saccharification enzyme has been inactivated is transferred to a filtration tank and then filtered by standing still.

상기 여과단계 이후, 여과 회수된 당화액에 호프를 첨가하고 자비시키는 자비단계(S400)는 여과를 거쳐 회수된 당화액에 호프를 첨가하고 자비시키는 단계이다. 자비는 당화액을 농축시키면서 호프의 쓴맛 성분 및 향기를 침출해 내고, 응고성 단백질이나 탄닌 결합물을 석출시키며, 효소의 활성을 중단시킨다.After the filtration step, the boiling step (S400) of adding and boiling hops to the saccharified solution recovered by filtration is a step of adding and boiling hops to the saccharified solution recovered through filtration. While boiling concentrates the saccharification solution, it leaches out the bitter taste components and aromas of hops, precipitates coagulant proteins or tannins, and stops enzyme activity.

호프(hop, humulus lupulus, L)는 뽕나무과에 속하는 자웅이주의 덩굴식물로서, 맥주의 원료로 사용되어 왔다. 호프는 루풀린(lupulin) 성분을 함유하고 있는데, 루풀린은 휴물렌(Humulen), 미르센(Myrcene), 휴물론(Humulon),루풀론(Lupulon), 호프레신 등으로 구성되어 있다. 루풀린의 독특한 향기와 쓴맛은 맥주에 풍미를 풍부하게 하고 쌉쌀한 맛을 제공한다. 또한, 방부의 효과와 항균작용, 진정작용 및 방부효과를 하여 맥주의 저장성을 높이는 효능이 있다. 또한, 호프에 함유된 탄닌도 루풀린과 비슷하게 맥주에서 쌉쌀한 맛을 내는 역할을 하며, 탄산과 잘 조화되어 맛을 좋게 하고 기름진 음식과 함께 섭취시 느끼함을 감소시키는 역할을 한다.Hop (humulus lupulus, L) is a hermaphrodite vine belonging to the family Morus and has been used as a raw material for beer. Hop contains lupulin, which is composed of Humulen, Myrcene, Humulon, Lupulon, and Hoppressin. Lupulin's unique aroma and bitter taste give beer a rich flavor and bitter taste. In addition, it has an antiseptic effect, an antibacterial action, a sedative action, and an antiseptic effect to increase the shelf life of beer. In addition, the tannins contained in hops also play a role in giving beer a bitter taste, similar to lupulin, and it harmonizes well with carbonic acid to improve the taste and reduce greasy feeling when consumed with oily foods.

한편, 본 발명은 바람직하게 여과액이 끓기 시작하면 비터 호프(bitter hop)를 첨가하고 60~120분 동안 자비시키는데, 맥주에 비터 호프(bitter hop)의 향과 맛을 충분히 가미하기 위해서이다. 또한, 아로마 호프(aromahop)는 끓임 종료 10~20분 전에 첨가하는 것이 좋다.Meanwhile, in the present invention, preferably, when the filtrate starts to boil, bitter hops are added and boiled for 60 to 120 minutes, in order to sufficiently add the flavor and flavor of bitter hops to beer. In addition, it is recommended to add aroma hop 10 to 20 minutes before the end of boiling.

한편, 자비 종료 후, 당화액의 최종 당도는 특정의 범위에 한정되는 것은 아니나, 바람직하게는 11~12 브릭스인 것이 좋다. 한편, 본 발명의 쌀맥주의 제조방법은, 바람직하게 자비 후, 침전물을 제거하는 단계를 추가적으로 포함하는 것이 좋다.On the other hand, after completion of boiling, the final sugar content of the saccharification liquid is not limited to a specific range, but preferably 11 to 12 Brix. On the other hand, the method for producing rice beer of the present invention, preferably after boiling, it is good to additionally include the step of removing the sediment.

상기 자비단계 이후, 효모를 첨가하고 발효시키는 발효단계(S500)는 자비 후, 효모를 첨가하고 발효시키는 단계이다. 효모는 자비를 거친 당화액에 함유되어 있는 발효성당을 이용하여 생장 및 번식을 하면서 맥주 제조시 필요한 알코올과 이산화탄소를 생성한다.After the boiling step, the fermentation step (S500) of adding yeast and fermenting is a step of adding yeast and fermenting after boiling. Yeast produces alcohol and carbon dioxide necessary for beer production while growing and propagating using the fermented sugar contained in the saccharified liquid that has undergone boiling.

한편, 발효는 특정의 방법에 반드시 한정되는 것은 아니나, 바람직하게는 12~18℃에서 발효시켜 알코올 함량이 4%가 되도록 1차 발효시키는 단계; 및, 1차 발효 후, 0℃에서 발효시켜 알코올 함량이 5%가 되도록 2차 발효시키는 단계로 수행하는 것이 좋은데, 알코올과 이산화탄소가 적당히 배합되어 풍미가 우수한 맥주를 제조할 수 있기 때문이다. On the other hand, the fermentation is not necessarily limited to a specific method, preferably, the step of fermenting at 12 ~ 18 ℃ primary fermentation so that the alcohol content is 4%; And, after primary fermentation, it is preferable to perform secondary fermentation so that the alcohol content is 5% by fermenting at 0 ° C. This is because beer with excellent flavor can be prepared by appropriately mixing alcohol and carbon dioxide.

이하, 앞서 설명드린 본 발명에 따른 쌀을 이용한 맥주 제조방법에 대하여 구체적인 실시예들을 제시하며, 보다 상세하게 설명드리도록 한다.Hereinafter, specific examples of the method for preparing beer using rice according to the present invention described above will be presented, and will be described in more detail.

<실시예1: 맥아 65중량%, 고시히까리 품종 생벼 35% 혼합>><Example 1: 65% by weight of malt, 35% of raw rice of Koshihikari variety>>

맥아 2600g과 고시히까리 품종 생벼 1400g을 각각 분쇄하여 각각의 분쇄된 원료를 4000g씩 혼합하였다. 전체 중량 55%에 해당하는 침지를 위한 주조용수 44L를 자비조에 투입하고, 가열하여 50℃가 되도록 한 후, 주조용수에 앞서 분쇄한 맥아와 고시히까리 품종 생벼 쌀을 투입하고, 주조용수의 온도를 50℃로 20분간 유지하여 당화에 필요한 효소를 용출시켰다. 효소를 용출시킨 후, 주조용수의 온도를 다시 65℃로 승온시켜 온도를 유지하면서 약 120분간 생벼의 전분을 호화시키면서 쌀 전분과 맥아의 전분을 발효성 당으로 전환하는 당화 과정을 수행하였다. 당화를 2시간 정도 진행하고, 요오드 테스트로서 당화된 정도를 확인한다. 당화 정도가 확인되면, 당화효소를 불활성화시키기 위하여, 주조용수 온도를 75℃ 이상으로 올리고 약 20분 동안 유지하여 당화를 중단시킨다.2600 g of malt and 1400 g of raw Koshihikari rice were ground, respectively, and each pulverized raw material was mixed with 4000 g each. 44L of casting water for immersion, equivalent to 55% of the total weight, is put into the boiling tank, heated to 50°C, and then ground malt and Koshihikari raw rice are added prior to the casting water, and the temperature of the casting water is set to 50 The enzyme required for saccharification was eluted by maintaining it at ℃ for 20 minutes. After the enzyme was eluted, the temperature of the brewing water was raised to 65° C., and the starch of raw rice was gelatinized for about 120 minutes while maintaining the temperature, and a saccharification process was performed to convert rice starch and malt starch into fermentable sugar. The saccharification is carried out for about 2 hours, and the degree of saccharification is confirmed by an iodine test. When the degree of saccharification is confirmed, in order to inactivate the saccharification enzyme, the temperature of the casting water is raised to 75° C. or higher and maintained for about 20 minutes to stop saccharification.

당화를 중단시킨 당화액을 여과조에 옮긴 후, 약 5분 정도 정치시켜 허스크를 이용해 여과층을 생성되면, 여과를 시작하여, 맑은 여과액이 나올 때까지 혼탁한 액은 다시 여과조로 되돌려서 다시 여과를 한다. 이후 맑은 여과액이 나오면 이를 자비조로 옮긴다. After stopping the saccharification, transfer the saccharification solution to the filtration tank, allow it to stand for about 5 minutes, and use a husk to form a filtration layer. Start filtration and return the turbid solution to the filtration tank until a clear filtrate comes out. filter After that, when a clear filtrate comes out, it is transferred to the boiling tank.

자비조의 여과액 온도를 95~100℃정도로 유지하여 여과액을 90분간 끓이는데, 여과액의 온도가 95℃ 이상이 되었을 때를 호프(bitter) 10g을 투입하고, 끊는 것이 끝나기 20분 전에 호프(aroma) 100g을 투입하였다. 끊는 과정이 종료된 상태에서 당화액의 최종당도는 12 브릭스이다. 자비가 끝나면 일정시간 정치 후, 단백질 응고물 등 슬러지를 원심분리을 이용해 20분간 침전시켜 제거하였다. 침전 공정이 완료되면 당화액의 온도를 열교환장치를 이용하여 15℃까지 냉각시켜 발효조로 이동시킨다. Boil the filtrate for 90 minutes by maintaining the temperature of the filtrate in the boiling tank at about 95~100℃. When the temperature of the filtrate reaches 95℃ or higher, add 10g of hops and 20 minutes before boiling ends. aroma) 100 g was added. When the boiling process is completed, the final sugar content of the saccharification solution is 12 Brix. After boiling, after standing for a certain period of time, sludge such as protein coagulated product was removed by precipitating it for 20 minutes by centrifugation. When the precipitation process is completed, the temperature of the saccharification liquid is cooled to 15° C. using a heat exchange device and transferred to the fermenter.

당화액이 절반 정도가 발효조로 운반되면 미리 활성시킨 효모를 발효조에 투입하였다. 효모는 건조효모 90g을 물에 혼합하여 24℃에서 24시간 동안 활성시키고, 당화액을 모두 발효조로 옮긴 후 발효조의 온도를 15℃로 유지하여 약 8일 동안 1차 발효를 진행한다. 1차 발효 기간 동안 발효조 내부의 가스 압력을 15~2psi 정도로 유지하며, 1차 발효가 종료된 맥주의 알코올 함량은 4%였가 되도록 한다. 1차 발효 종료 후, 발효조의 온도를 5℃로 낮추고 18시간을 방치한 뒤 효모를 제거하고, 발효조의 온도를 5℃로 유지하여 2차 발효를 수행한다. 2차 발효 과정은 약 2주간 수행하였고, 2차 발효를 마친 맥주의 알코올 함량은 5%가 되도록 한다.When about half of the saccharification liquid was transported to the fermenter, the previously activated yeast was added to the fermenter. Yeast is activated by mixing 90 g of dry yeast in water at 24° C. for 24 hours, and after transferring all the saccharification solution to the fermenter, the temperature of the fermenter is maintained at 15° C. and primary fermentation is performed for about 8 days. During the primary fermentation period, the gas pressure inside the fermenter is maintained at about 15 to 2 psi, and the alcohol content of the beer after the primary fermentation is completed is 4%. After completion of the primary fermentation, the temperature of the fermenter was lowered to 5° C., left for 18 hours, the yeast was removed, and the temperature of the fermenter was maintained at 5° C. to perform secondary fermentation. The secondary fermentation process was carried out for about 2 weeks, and the alcohol content of the beer after the secondary fermentation was set to 5%.

<비교예1: 맥아 100중량%, 생벼 쌀 포함하지 않은 혼합>><Comparative Example 1: 100% by weight malt, mixed without raw rice>>

맥아 4000g을 분쇄하여 각각의 분쇄된 원료를 4000g씩 혼합하였다. 전체 중량 55%에 해당하는 침지를 위한 주조용수 44L를 자비조에 투입하고, 가열하여 50℃가 되도록 한 후, 주조용수에 앞서 분쇄한 맥아와 고시히까리 품종 생벼 쌀을 투입하고, 주조용수의 온도를 50℃로 20분간 유지하여 당화에 필요한 효소를 용출시켰다. 이하 과정은 실시예1과 동일하므로, 자세할 설명은 생략하기로 한다.4000 g of malt was pulverized and each pulverized raw material was mixed by 4000 g. 44L of casting water for immersion, equivalent to 55% of the total weight, is put into the boiling tank, heated to 50°C, and then ground malt and Koshihikari raw rice are added prior to the casting water, and the temperature of the casting water is set to 50 The enzyme required for saccharification was eluted by maintaining it at ℃ for 20 minutes. Since the following process is the same as that of Example 1, a detailed description thereof will be omitted.

<비교예2: 맥아 75중량%, 고시히까리 품종 생벼 25량% 혼합>><Comparative Example 2: 75% by weight of malt, 25% by weight of raw rice of Koshihikari variety>>

맥아 3000g과 고시히까리 품종 생벼 1000g을 각각 분쇄하여 각각의 분쇄된 원료를 4000g씩 혼합하였다. 전체 중량 55%에 해당하는 침지를 위한 주조용수 44L를 자비조에 투입하고, 가열하여 50℃가 되도록 한 후, 주조용수에 앞서 분쇄한 맥아와 고시히까리 품종 생벼 쌀을 투입하고, 주조용수의 온도를 50℃로 20분간 유지하여 당화에 필요한 효소를 용출시켰다. 이하 과정은 실시예1과 동일하므로, 자세할 설명은 생략하기로 한다.3000 g of malt and 1000 g of raw Koshihikari rice were ground, respectively, and each pulverized raw material was mixed with 4000 g each. 44L of casting water for immersion, equivalent to 55% of the total weight, is put into the boiling tank, heated to 50°C, and then ground malt and Koshihikari raw rice are added prior to the casting water, and the temperature of the casting water is set to 50 The enzyme required for saccharification was eluted by maintaining it at ℃ for 20 minutes. Since the following process is the same as that of Example 1, a detailed description thereof will be omitted.

<비교예3: 맥아 55중량%, 고시히까리 품종 생벼 45량% 혼합>><Comparative Example 3: 55% by weight of malt, 45% by weight of raw rice of Koshihikari variety>>

맥아 2200g과 고시히까리 품종 생벼 1800g을 각각 분쇄하여 각각의 분쇄된 원료를 4000g씩 혼합하였다. 전체 중량 55%에 해당하는 침지를 위한 주조용수 44L를 자비조에 투입하고, 가열하여 50℃가 되도록 한 후, 주조용수에 앞서 분쇄한 맥아와 고시히까리 품종 생벼 쌀을 투입하고, 주조용수의 온도를 50℃로 20분간 유지하여 당화에 필요한 효소를 용출시켰다. 이하 과정은 실시예1과 동일하므로, 자세할 설명은 생략하기로 한다.2200 g of malt and 1800 g of raw Koshihikari rice were ground, respectively, and each pulverized raw material was mixed with 4000 g each. 44L of casting water for immersion, equivalent to 55% of the total weight, is put into the boiling tank, heated to 50°C, and then ground malt and Koshihikari raw rice are added prior to the casting water, and the temperature of the casting water is set to 50 The enzyme required for saccharification was eluted by maintaining it at ℃ for 20 minutes. Since the following process is the same as that of Example 1, a detailed description thereof will be omitted.

<비교예4: 맥아 65중량%, 새누리 품종 생벼 35량% 혼합>><Comparative Example 4: 65% by weight malt, 35% by weight of raw rice of Saenuri variety>>

맥아 2600g과 새누리 품종 생벼 1400g을 각각 분쇄하여 각각의 분쇄된 원료를 4000g씩 혼합하였다. 전체 중량 55%에 해당하는 침지를 위한 주조용수 44L를 자비조에 투입하고, 가열하여 50℃가 되도록 한 후, 주조용수에 앞서 분쇄한 맥아와 고시히까리 품종 생벼 쌀을 투입하고, 주조용수의 온도를 50℃로 20분간 유지하여 당화에 필요한 효소를 용출시켰다. 이하 과정은 실시예1과 동일하므로, 자세할 설명은 생략하기로 한다.2600 g of malt and 1400 g of raw rice of the Saenuri variety were crushed, respectively, and 4000 g of each pulverized raw material was mixed. 44L of casting water for immersion, equivalent to 55% of the total weight, is put into the boiling tank, heated to 50°C, and then ground malt and Koshihikari raw rice are added prior to the casting water, and the temperature of the casting water is set to 50 The enzyme required for saccharification was eluted by maintaining it at ℃ for 20 minutes. Since the following process is the same as that of Example 1, a detailed description thereof will be omitted.

이하 위의 실시예1과 비교예 1 내지 4의 각각에 대한 향기 성분 분석을 한다. 향기 성분 분석의 방법으로 맥주의 향기 성분추출에 SAFE(Solvent Assisted Flavor evaporation) 장치를 사용하되, 내부표준물질은 1-도데카놀(1-Dodecanol)을 사용하며, 용매로 디클로로메탄(dichloromathane)을 사용하여 1-도데카놀(1-Dodecanol) 400㎕를 2ml로 희석하여 사용한다. Hereinafter, the fragrance component analysis for each of Example 1 and Comparative Examples 1 to 4 above. As a method of fragrance component analysis, SAFE (Solvent Assisted Flavor evaporation) is used to extract the aroma components of beer, but 1-dodecanol is used as the internal standard material, and dichloromathane is used as the solvent. Thus, 400 μl of 1-dodecanol is diluted to 2 ml and used.

이에 각 실시예1과 비교예 1 내지 4의 분석할 맥주 시료 100ml에 내부표준물질의 희석액 5㎕를 첨가하여 SAFE장치로 향기성분을 추출하였다. SAFE 장치내부에 감압을 가하고 준비된 맥주 시료를 증류 플라스크에 넣고 수욕 상에서 45℃로 가열하였다. 감압가열 하에서 향기성분은 증류되어 증류 플라스크 반대편에 있는 플라스크에 액체질소에 의해서 냉각 포집되었고, 비휘발성 성분은 증류 플라스크에 남게 되었다. 냉각 포집된 향기성분은 상온에서 녹인 후, 50ml의 디클로로메탄(dichloromethane)과 함께 삼각플라스크에 옮겨 담고 1시간의 교반 과정을 통하여 향기성분을 디클로로메탄(dichloromethane)에 흡수시켰다. 분별 깔때기를 이용하여 디클로로메탄(dichloromethane) 층을 물 층과 분리하였고, 분리해낸 디클로로메탄(dichloromethane) 층에 소디움 설페이트(Sodium sulfate)를 가한 다음, 12시간 동안 5℃의 냉장고에 보관하며 잔여 수분을 제거하였다. 수분을 제거하고 시료가 담긴 플라스크에 비고루스 컬럼(vigorous column)을 연결하고, 시료를 50℃에서 1ml 수준으로 농축한 후, 다시 질소 충진을 통하여 04ml까지 농축하였다. Accordingly, 5 μl of a diluted solution of an internal standard was added to 100 ml of the beer samples to be analyzed in Examples 1 and 1 to 4, and the fragrance component was extracted with a SAFE device. A reduced pressure was applied to the inside of the SAFE apparatus, and the prepared beer sample was placed in a distillation flask and heated to 45° C. in a water bath. The aromatic components were distilled under reduced pressure heating and collected by cooling with liquid nitrogen in a flask opposite the distillation flask, and nonvolatile components remained in the distillation flask. After cooling the captured fragrance component was dissolved at room temperature, it was transferred to an Erlenmeyer flask along with 50 ml of dichloromethane, and the fragrance component was absorbed in dichloromethane through a stirring process of 1 hour. The dichloromethane layer was separated from the water layer using a separatory funnel, and sodium sulfate was added to the separated dichloromethane layer, and then stored in a refrigerator at 5° C. for 12 hours and the remaining moisture was removed. removed. After removing moisture and connecting a vigorous column to the flask containing the sample, the sample was concentrated to 1 ml at 50° C., and then concentrated to 04 ml through nitrogen filling again.

분리된 향기 성분의 분석은 GC-MS를 사용하였다. GC의 분석조건으로 컬럼은 DB-wax를 사용하였고, 인젝터(injector) 온도는 250℃, 오븐 온도는 50℃에서 2분간 유지한 다음, 분당 3℃로 200℃까지 올린 후 250℃에서 10분간 유지하도록 하였으며, 운반기체는 헬륨을 사용하였고, 시료는 스플리트리스 모드(splitless mode)로 1㎕ 주입하였다.GC-MS was used for the analysis of the separated fragrance component. For GC analysis conditions, DB-wax was used for the column, and the injector temperature was 250°C and the oven temperature was maintained at 50°C for 2 minutes, then raised to 200°C at 3°C per minute and maintained at 250°C for 10 minutes. Helium was used as the carrier gas, and 1 μl of the sample was injected in splitless mode.

향기성분 정성을 위한 MS의 분석조건으로 'MS ioniztion voltage'는 70 eV, 소스 온도(source temperature)는 280℃, 매스 스펙트럼 범위(mass spectrum range)는 50-550m/z로 하였다. 향기성분은 'Kovatgas chromatographic retention index I'와 실제 화합물과 비교한 후 각 성분의 'Mass Spectrometry framention, Willy 6th edition MS spectra library'와 실제 성분과 비교하여 정성하였다. 향기성분의 양은 국제적인 스탠다드(International standard)로 쓰인 1-도데카놀(1-Dodecanol) 외 피크면적과 각 화합물들 간의 피크면적 간의 비(피크 비율, Peak Ratio)로 나타내었다.As the analysis conditions for MS for fragrance component quality, the 'MS ioniztion voltage' was 70 eV, the source temperature was 280°C, and the mass spectrum range was 50-550m/z. Fragrance components were qualitatively compared with 'Kovatgas chromatographic retention index I' and actual compounds, and then compared with each component's 'Mass Spectrometry framention, Willy 6th edition MS spectra library' and actual components. The amount of the fragrance component was expressed as the ratio (peak ratio, Peak Ratio) between the peak area of each compound and the peak area other than 1-Dodecanol, which was used as an international standard.

실시예 1 맥아 65중량%, 고시히까리 품종 생벼 35% 맥주 향기 분석 결과Example 1 Malt 65% by weight, Koshihikari variety raw rice 35% beer aroma analysis result 구분division 화합물성분compound component 코바트
(kovat)
kobart
(kovat)
코바트기준
(kovat Ref.)
Kobart standards
(kovat Ref.)
피크비율
(Peak ratio)
peak ratio
(Peak ratio)
1One ethyl butanoateethyl butanoate 1029 1029 1029 1029 0.0327±0.00320.0327±0.0032 22 isobutyl alcoholisobutyl alcohol 1082 1082 1084 1084 0.0354±0.0550.0354±0.055 33 isoamyl acetateisoamyl acetate 1121 1121 1118 1118 0.0635±0.0230.0635±0.023 44 1-butanol1-butanol 1215 1215 1138 1138 2.2636±0.5052.2636±0.505 55 isoamyl aclcoholisoamyl aclcohol 1236 1236 1205 1205 0.0335±0.0050.0335±0.005 66 ethyl hexanoateethyl hexanoate 1357 1357 1229 1229 0.0082±0.00250.0082±0.0025 77 3-methyl-but-3-en-1-ol3-methyl-but-3-en-1-ol 1433 1433 1244 1244 0.0333±0.0220.0333±0.022 88 1-hexanol1-hexanol 1444 1444 1350 1350 0.228±0.03330.228±0.0333 99 ethyl octanoateethyl octanoate 1459 1459 1430 1430 0.0039±0.00050.0039±0.0005 1010 acetic acidacetic acid 1465 1465 1475 1475 0.0023±0.0000.0023±0.000 1111 1-heptanol1-heptanol 1522 1522 1454 1454 0.0032±0.00530.0032±0.0053 1212 benzaldehydebenzaldehyde 1534 1534 1516 1516 0.0538±0.0350.0538±0.035 1313 propanoic acidpropanoic acid 1539 1539 1535 1535 0.0434±0.0330.0434±0.033 1414 linaloollinalool 1562 1562 1546 1546 0.0383±0.0230.0383±0.023 1515 2-methylpropanoic acid2-methylpropanoic acid 1617 1617 1568 1568 0.0395±0.0230.0395±0.023 1616 5,5-dimethyl-2(5H)-furanone5,5-dimethyl-2(5H)-furanone 1623 1623 1590 1590 0.0253±0.0030.0253±0.003 1717 butanoic acidbutanoic acid 1638 1638 1650 1650 0.3243±0.0030.3243±0.003 1818 γ-buyrolactone γ-buyrolactone 16811681 1623 1623 0.0033±0.0000.0033±0.000 1919 3-methylbutanoic acid3-methylbutanoic acid 1731 1731 1680 1680 0.0082±0.0020.0082±0.002 2020 γ-hexalactone γ-hexalactone 1816 1816 1669 1669 0.0753±0.0230.0753±0.023 2121 pentanoic acidpentanoic acid 1839 1839 1750 1750 0.2507±0.2060.2507±0.206 2222 phenyl ethylacetatephenyl ethylacetate 1878 1878 1813 1813 0.0325±0.0020.0325±0.002 2323 hexanoic acidhexanoic acid 1919 1919 1821 1821 3.33±0.5883 3.33±0.5883 2424 phenyl ethylalcoholphenyl ethyl alcohol 1973 1973 1910 1910 1One 2525 1-dodecanol1-dodecanol 2056 2056 1969 1969 0.5605±0.0300.5605±0.030 2626 γ-nonalactone γ-nonactone 2138 2138 2030 2030 0.025±0.02330.025±0.0233 2727 octanoic acidoctanoic acid 2197 2197 2047 2047 0.0526±0.0060.0526±0.006 2828 γ-decalactoneγ-decalactone 2268 2268 2147 2147 0.2583±0.0220.2583±0.022 2929 nonanoic acidnonanoic acid 2424 2424 2260 2260 0.0333±0.0030.0333±0.003 3030 2-methoxy-4-vinylphenol2-methoxy-4-vinylphenol 2480 2480 2195 2195 0.0383±0.0200.0383±0.020 3131 decanoic aciddecanoic acid 2266 2266 2400 2400 0.0525±0.0320.0525±0.032 3232 benzoic acidbenzoic acid 2421 2421 2410 2410 0.0332±0.0320.0332±0.032 3333 odecanoic acidodecanoic acid 2476 2476 2500 2500 0.0573±0.0380.0573±0.038 3434 tetradecanoic acidtetradecanoic acid 2690 2690 2790 2790 0.0543±0.0360.0543±0.036

비교예 1 맥아 100중량%, 생벼 쌀 포함하지 않은 맥주 향기 분석 결과Comparative Example 1 100% by weight of malt, analysis result of beer aroma without raw rice 구분division 화합물성분compound component 코바트
(kovat)
kobart
(kovat)
코바트기준
(kovat Ref.)
Kobart standards
(kovat Ref.)
피크비율
(Peak ratio)
peak ratio
(Peak ratio)
1One ethyl butanoateethyl butanoate 1029 1029 1029 1029 0.0228±0.00240.0228±0.0024 22 isobutyl alcoholisobutyl alcohol 1082 1082 1084 1084 0.0741±0.0540.0741±0.054 33 isoamyl acetateisoamyl acetate 1121 1121 1118 1118 0.0974±0.0160.0974±0.016 44 isoamyl aclcoholisoamyl aclcohol 1215 1215 1138 1138 2.1974±0.4052.1974±0.405 55 ethyl hexanoateethyl hexanoate 1236 1236 1205 1205 0.0275±0.0050.0275±0.005 66 1-hexanol1-hexanol 1357 1357 1229 1229 0.008±0.00150.008±0.0015 77 ethyl octanoateethyl octanoate 1433 1433 1244 1244 0.0732±0.0110.0732±0.011 88 acetic acidacetic acid 1444 1444 1350 1350 0.118±0.02620.118±0.0262 99 1-heptanol1-heptanol 1459 1459 1430 1430 0.003±0.00050.003±0.0005 1010 furfuralfurfural 1465 1465 1475 1475 0.0012±0.0000.0012±0.000 1111 benzaldehydebenzaldehyde 1522 1522 1454 1454 0.007±0.00470.007±0.0047 1212 propanoic acidpropanoic acid 1534 1534 1516 1516 0.0538±0.0340.0538±0.034 1313 furfuryl acetatefurfuryl acetate 1539 1539 1535 1535 0.0467±0.0330.0467±0.033 1414 2-methylpropanoic acid2-methylpropanoic acid 1562 1562 1546 1546 0.0383±0.0170.0383±0.017 1515 5,5-dimethyl-2(5H)-furanone5,5-dimethyl-2(5H)-furanone 1617 1617 1568 1568 0.0374±0.0170.0374±0.017 1616 butanoic acidbutanoic acid 1623 1623 1590 1590 0.0147±0.0030.0147±0.003 1717 γ-buyrolactone γ-buyrolactone 16381638 1650 1650 0.2142±0.0030.2142±0.003 1818 ethyl succinateethyl succinate 1681 1681 1623 1623 0.0033±0.0000.0033±0.000 1919 pentanoic acidpentanoic acid 1731 1731 1680 1680 0.0082±0.0010.0082±0.001 2020 phenyl ethylacetatephenyl ethylacetate 1816 1816 1669 1669 0.0957±0.0170.0957±0.017 2121 hexanoic acidhexanoic acid 1839 1839 1750 1750 0.1501±0.1090.1501±0.109 2222 benzyl alcoholbenzyl alcohol 1878 1878 1813 1813 0.0214±0.0010.0214±0.001 2323 phenyl ethylalcoholphenyl ethyl alcohol 1919 1919 1821 1821 3.23±0.5886 3.23±0.5886 2424 dodecanol dodecanol 1973 1973 1910 1910 1 One 2525 octanoic acidoctanoic acid 2056 2056 1969 1969 0.4704±0.0700.4704±0.070 2626 ethyl cinamateethyl cinamate 2138 2138 2030 2030 0.015±0.01720.015±0.0172 2727 2-methoxy-4-vinylphenol2-methoxy-4-vinylphenol 2197 2197 2047 2047 0.0519±0.0090.0519±0.009 2828 decanoic aciddecanoic acid 2268 2268 2147 2147 0.1443±0.0110.1443±0.011 2929 benzoic acidbenzoic acid 2424 2424 2260 2260 0.0373±0.0070.0373±0.007 3030 dodecanoic aciddodecanoic acid 2480 2480 2195 2195 0.0372±0.0100.0372±0.010

우선, 실시예1과 비교예1의 맥주의 향기성분을 분석한 결과, 실시예 1(표 1)은 34가지의 향기 성분들이 분석되었고, 비교예 1(표2)은 30의 향기 성분이 분석되었다. 또한, 실시예1(표1)에서는 비교예 1(표2)에서는 검출되지 않았던 γ-헥사락톤(γ-hexalactone), γ-노나락톤(γ-nonalactone), γ-데카락톤(γ-decalactone) 등의 락톤(lactone)류의 화합물들과 1- 부타놀(1-butanol), 3- 케닐-부-3-엔-1-올(3-methyl-but-3-en-1-ol), 3- 메틸부탄올(3-methylbutanol) 등의 향기성분들이 검출되어, 실시예 1(표1)에서 풍부한 향미를 가지는 것이 확인되었다.First, as a result of analyzing the aroma components of the beer of Example 1 and Comparative Example 1, 34 types of aroma components were analyzed in Example 1 (Table 1), and 30 aroma components were analyzed in Comparative Example 1 (Table 2). became In addition, in Example 1 (Table 1), γ-hexalactone, γ-nonalactone, γ-decalactone, which were not detected in Comparative Example 1 (Table 2), γ-decalactone ) and other lactone compounds and 1-butanol, 3-kenyl-but-3-en-1-ol , 3-methylbutanol (3-methylbutanol) and the like were detected, and it was confirmed that it had a rich flavor in Example 1 (Table 1).

비교예 2 맥아 75중량%, 고시히까리 품종 생벼 25량% 맥주 향기 분석 결과Comparative Example 2 Malt 75% by weight, Koshihikari variety raw rice 25% by weight Beer aroma analysis result 구분division 화합물성분compound component 코바트
(kovat)
kobart
(kovat)
코바트기준
(kovat Ref.)
Kobart standards
(kovat Ref.)
피크비율
(Peak ratio)
peak ratio
(Peak ratio)
1One ethyl butanoateethyl butanoate 10191019 1029 1029 0.0336±0.00340.0336±0.0034 22 isobutyl alcoholisobutyl alcohol 10631063 1084 1084 0.0541±0.0740.0541±0.074 33 isoamyl acetateisoamyl acetate 11111111 1118 1118 0.0777±0.0160.0777±0.016 44 1-butanol1-butanol 11861186 1138 1138 2.0976±0.4052.0976±0.405 55 isoamyl aclcoholisoamyl aclcohol 12261226 1205 1205 0.0289±0.0150.0289±0.015 66 ethyl hexanoateethyl hexanoate 13401340 1229 1229 0.0081±0.01250.0081±0.0125 77 3-methyl-but-3-en-1-ol3-methyl-but-3-en-1-ol 13921392 1244 1244 0.0533±0.0320.0533±0.032 88 1-hexanol1-hexanol 14301430 1350 1350 0.128±0.02640.128±0.0264 99 ethyl octanoateethyl octanoate 14491449 1430 1430 0.021±0.00040.021±0.0004 1010 acetic acidacetic acid 14551455 1475 1475 0.0018±0.00010.0018±0.0001 1111 1-heptanol1-heptanol 15121512 1454 1454 0.0074±0.00550.0074±0.0055 1212 benzaldehydebenzaldehyde 15231523 1516 1516 0.0432±0.0330.0432±0.033 1313 propanoic acidpropanoic acid 15291529 1535 1535 0.0439±0.0320.0439±0.032 1414 linaloollinalool 15521552 1546 1546 0.0291±0.0240.0291±0.024 1515 2-methylpropanoic acid2-methylpropanoic acid 16071607 1568 1568 0.0476±0.0160.0476±0.016 1616 5,5-dimethyl-2(5H)-furanone5,5-dimethyl-2(5H)-furanone 16031603 1590 1590 0.0158±0.0020.0158±0.002 1717 butanoic acidbutanoic acid 16281628 1650 1650 0.2241±0.00280.2241±0.0028 1818 γ-buyrolactoneγ-buyrolactone 16711671 1623 1623 0.0045±0.00010.0045±0.0001 1919 3-methylbutanoic acid3-methylbutanoic acid 17161716 1680 1680 0.0096±0.00150.0096±0.0015 2020 γ-hexalactoneγ-hexalactone 17431743 1669 1669 0.0887±0.0280.0887±0.028 2121 oentanoic acidoentanoic acid 18191819 1750 1750 0.1612±0.1160.1612±0.116 2222 phenyl ethylacetatephenyl ethylacetate 18681868 1813 1813 0.0313±0.0020.0313±0.002 2323 hexanoic acidhexanoic acid 18891889 1821 1821 2.23±0.4212 2.23±0.4212 2424 phenyl ethylalcoholphenyl ethyl alcohol 19631963 1910 1910 1 One 2525 1-dodecanol1-dodecanol 20382038 1969 1969 0.4552±0.0650.4552±0.065 2626 γ-nonalactoneγ-nonactone 21052105 2030 2030 0.0213±0.02230.0213±0.0223 2727 octanoic acidoctanoic acid 21872187 2047 2047 0.0425±0.0080.0425±0.008 2828 γ-decalactoneγ-decalactone 22532253 2147 2147 0.1555±0.0190.1555±0.019 2929 nonanoic acidnonanoic acid 23112311 2260 2260 0.0404±0.0060.0404±0.006 3030 2-methoxy-4-vinylphenol2-methoxy-4-vinylphenol 24702470 2195 2195 0.0386±0.0210.0386±0.021 3131 decanoic aciddecanoic acid 22562256 2400 2400 0.0456±0.0320.0456±0.032 3232 benzoic acidbenzoic acid 24112411 2410 2410 0.0377±0.0390.0377±0.039 3333 dodecanoic aciddodecanoic acid 24662466 2500 2500 0.0511±0.0350.0511±0.035 3434 tetradecanoic acidtetradecanoic acid 24702470 2790 2790 0.0489±0.0220.0489±0.022

비교예 3 맥아 55중량%, 고시히까리 품종 생벼 45량% 맥주 향기 분석 결과Comparative Example 3 55% by weight of malt, 45% by weight of raw rice of Koshihikari variety Beer aroma analysis result 구분division 화합물성분compound component 코바트
(kovat)
kobart
(kovat)
코바트기준
(kovat Ref.)
Kobart standards
(kovat Ref.)
피크비율
(Peak ratio)
peak ratio
(Peak ratio)
1One ethyl butanoateethyl butanoate 1030 1030 1029 1029 0.0199±0.00320.0199±0.0032 22 isobutyl alcoholisobutyl alcohol 1075 1075 1084 1084 0.0241±0.0430.0241±0.043 33 isoamyl acetateisoamyl acetate 1121 1121 1118 1118 0.0624±0.0190.0624±0.019 44 1-butanol1-butanol 1215 1215 1138 1138 2.1624±0.4052.1624±0.405 55 isoamyl aclcoholisoamyl aclcohol 1216 1216 1205 1205 0.0224±0.0110.0224±0.011 66 ethyl hexanoateethyl hexanoate 1357 1357 1229 1229 0.008±0.00170.008±0.0017 77 3-methyl-but-3-en-1-ol3-methyl-but-3-en-1-ol 1433 1433 1244 1244 0.0222±0.0120.0222±0.012 88 1-hexanol1-hexanol 1444 1444 1350 1350 0.118±0.02250.118±0.0225 99 ethyl octanoateethyl octanoate 1459 1459 1430 1430 0.002±0.00130.002±0.0013 1010 acetic acidacetic acid 1465 1465 1475 1475 0.0012±0.0110.0012±0.011 1111 1-heptanol1-heptanol 1522 1522 1454 1454 0.002±0.00420.002±0.0042 1212 benzaldehydebenzaldehyde 1534 1534 1516 1516 0.0428±0.0240.0428±0.024 1313 propanoic acidpropanoic acid 1539 1539 1535 1535 0.0422±0.0220.0422±0.022 1414 linaloollinalool 1562 1562 1546 1546 0.0282±0.0120.0282±0.012 1515 2-methylpropanoic acid2-methylpropanoic acid 1617 1617 1568 1568 0.0224±0.0120.0224±0.012 1616 5,5-dimethyl-2(5H)-furanone5,5-dimethyl-2(5H)-furanone 1623 1623 1590 1590 0.0142±0.0020.0142±0.002 1717 butanoic acidbutanoic acid 1638 1638 1650 1650 0.2142±0.0020.2142±0.002 1818 γ-buyrolactoneγ-buyrolactone 1681 1681 1623 1623 0.0022±0.0020.0022±0.002 1919 3-methylbutanoic acid3-methylbutanoic acid 1731 1731 1680 1680 0.0082±0.00120.0082±0.0012 2020 γ-hexalactoneγ-hexalactone 1816 1816 1669 1669 0.0642±0.0120.0642±0.012 2121 pentanoic acidpentanoic acid 1839 1839 1750 1750 0.1401±0.1060.1401±0.106 2222 phenyl ethylacetatephenyl ethylacetate 1857 1857 1813 1813 0.0214±0.0010.0214±0.001 2323 hexanoic acidhexanoic acid 1919 1919 1821 1821 2.22±0.4882 2.22±0.4882 2424 phenyl ethylalcoholphenyl ethyl alcohol 1913 1913 1910 1910 1 One 2525 1-dodecanol1-dodecanol 2056 2056 1969 1969 0.4204±0.02010.4204±0.0201 2626 γ-nonalactoneγ-nonactone 2138 2138 2030 2030 0.014±0.01220.014±0.0122 2727 octanoic acidoctanoic acid 2197 2197 2047 2047 0.0416±0.0060.0416±0.006 2828 γ-decalactoneγ-decalactone 2268 2268 2147 2147 0.1442±0.01120.1442±0.0112 2929 nonanoic acidnonanoic acid 2424 2424 2260 2260 0.0222±0.0020.0222±0.002 3030 2-methoxy-4-vinylphenol2-methoxy-4-vinylphenol 2480 2480 2195 2195 0.0222±0.01040.0222±0.0104 3131 decanoic aciddecanoic acid 2266 2266 2400 2400 0.0414±0.02120.0414±0.0212 3232 benzoic acidbenzoic acid 2421 2421 2410 2410 0.0221±0.02130.0221±0.0213 3333 dodecanoic aciddodecanoic acid 2476 2476 2500 2500 0.0442±0.0280.0442±0.028 3434 tetradecanoic acidtetradecanoic acid 2690 2690 2790 2790 0.0442±0.0260.0442±0.026

에틸 아세테이트(Ethyl acetate) 등Ethyl acetate, etc.

위와 같이 실시예1과 비교예 2 내지 3의 맥주의 향기성분을 분석한 결과, 실시예 1(표 1)과 비교예 2 내지 3에서 모두 34가지의 향기 성분들이 인출되었다.As a result of analyzing the aroma components of the beer of Example 1 and Comparative Examples 2 to 3 as above, in Example 1 (Table 1) and Comparative Examples 2 to 3, all 34 types of aroma components were withdrawn.

그러나, 실시예1과 달리, 비교예 2에서 고사히끼리 생벼 쌀을 비율을 45%로 높이는 경우 비교예1(표2)에서 검출되지 않은 고사히끼리 생벼 쌀의 혼합 투입으로 인하여 인출되는 향기 성분들인 γ-헥사락톤(γ-hexalactone), γ-노나락톤(γ-nonalactone), γ-데카락톤(γ-decalactone) 등의 락톤(lactone)류의 화합물들과 1- 부타놀(1-butanol), 3- 케닐-부-3-엔-1-올(3-methyl-but-3-en-1-ol), 3- 메틸부탄올(3-methylbutanol) 등의 수치는 높게 나타났으나, 맥주 본연의 맥아의 투입으로 인하여 인출되는 맥주의 일반적인 특징 향기 성분인 페닐 에틸 알코올(Phenyl ethyl alcohol), 페닐 에틸 알코올(Phenyl ethyl alcohol), 이소아밀 알코올(Isoamyl alcohol), 이소부틸 알코올(Isobutyl alcohol) 등의 알콜류와, 페닐 에틸 아세테이트(Phenyl ethyl acetate) 등의 에스테르화합물류, 부타노일 엑시드(Butanoic acid) 등의 유기산 종류가 감소되어 맥주로서의 특성을 잃어버리는 단점을 확인할 수 있다.However, unlike Example 1, when the ratio of raw Kosahiki raw rice in Comparative Example 2 is increased to 45%, the fragrance component extracted due to the mixed input of Kosahiki raw rice not detected in Comparative Example 1 (Table 2) Lactone compounds such as γ-hexalactone, γ-nonalactone, and γ-decalactone and 1-butanol ), 3-kenyl-but-3-en-1-ol (3-methyl-but-3-en-1-ol), and 3-methylbutanol (3-methylbutanol) were high, but beer General characteristics of beer extracted due to the original malt input Phenyl ethyl alcohol, Phenyl ethyl alcohol, Isoamyl alcohol, Isobutyl alcohol, etc. Alcohols, ester compounds such as phenyl ethyl acetate, and organic acids such as butanoic acid are reduced, so the disadvantage of losing the characteristics of beer can be confirmed.

또한, 실시예1과 달리, 비교예3과 같이 고사히끼리 생벼 쌀의 비율을 25%로 낮출 경우에는 고사히끼리 생벼 쌀의 투입으로 인출되는 γ-헥사락톤(γ-hexalactone), γ-노나락톤(γ-nonalactone), γ-데카락톤(γ-decalactone) 등의 락톤(lactone)류의 화합물들과 1- 부타놀(1-butanol), 3- 케닐-부-3-엔-1-올(3-methyl-but-3-en-1-ol), 3- 메틸부탄올(3-methylbutanol) 등 특유의 향기 성분들의 수치가 매우 낮아져, 본 발명의 목적인 특유의 풍미를 가미한 맥주로서의 장점을 잃어버리는 것이 확인된다.In addition, unlike Example 1, when the ratio of raw rice with dead rice is lowered to 25% as in Comparative Example 3, γ-hexalactone and γ-no Lactone compounds such as γ-nonalactone and γ-decalactone and 1-butanol, 3-kenyl-but-3-en-1- The level of unique fragrance components, such as 3-methyl-but-3-en-1-ol and 3-methylbutanol, is very low, so the advantage as a beer with a unique flavor, which is the object of the present invention, has been improved. lost is confirmed.

이상과 같이, 본 발명에 따른 쌀맥주 제조방법은 일반쌀보다 영양성분이 매우 우수한 고시히카리 품종 생벼 쌀을 이용하여, 새로운 풍미와 크리스피한 맛을 제공하며 드라이한 스타일의 고시히카리 품종 쌀을 이용한 고급 품질의 맥주를 제조할 수 있다.As described above, the rice beer manufacturing method according to the present invention uses raw Koshihikari rice, which has a very superior nutritional component than normal rice, and provides a new flavor and crispy taste, and is of high quality using dry style Koshihikari rice. Beer can be brewed.

삭제delete

Claims (6)

맥아 65중량%와 고시히까리 품종 생벼 35중량%을 혼합하고, 50℃의 물에 침지시켜 당화효소를 용출시키는 용출단계;
상기 용출단계 이후, 65℃에서 호화 및 당화 반응으로 당화시키는 당화단계;
상기 당화단계 이후, 당화효소를 불활성화시키고 여과하는 여과단계;
상기 여과단계 이후, 여과 회수된 당화액에 호프를 첨가하고 자비시키는 자비단계; 및
상기 자비단계 이후, 효모를 첨가하고 발효시키는 발효단계를 포함하고,
상기 발효단계에서,
15℃에서 발효시켜 알코올 함량이 4%가 되도록 발효를 시키는 1차 발효 단계; 및 저온인 5℃에서 발효시켜 알코올 함량이 5%가 되도록 발효시키는 2차 발효단계를 특징으로 하고,
상기 용출단계에서,
상기 맥아와 고시히까리 품종벼로 조성된 홉합곡물량 대비 500~600중량%의 물에 침지시키는 것을 특징으로 하고,
상기 용출단계에서,
15 ~ 20분 동안 침지시키는 것을 특징으로 하고,
상기 당화단계에서,
100 ~ 150분 동안 호화 및 당화 반응을 진행하는 것을 특징으로 하는 쌀을 이용한 맥주 제조방법.
An elution step of mixing 65% by weight of malt and 35% by weight of raw Koshihikari rice, and immersing it in water at 50° C. to elute the saccharifying enzyme;
After the elution step, a saccharification step of saccharification by gelatinization and saccharification reaction at 65°C;
After the saccharification step, a filtration step of inactivating the saccharification enzyme and filtering;
After the filtration step, boiling step of adding hops to the saccharified solution recovered by filtration and boiling; and
After the boiling step, it includes a fermentation step of adding yeast and fermenting,
In the fermentation step,
The primary fermentation step of fermentation at 15° C. so that the alcohol content is 4%; and a secondary fermentation step in which the alcohol content is fermented to 5% by fermentation at a low temperature of 5°C,
In the elution step,
It is characterized in that it is immersed in water in an amount of 500 to 600% by weight compared to the amount of hop grains composed of the malt and Koshihikari rice,
In the elution step,
characterized in that it is immersed for 15 to 20 minutes,
In the saccharification step,
A method for producing beer using rice, characterized in that gelatinization and saccharification reactions are performed for 100 to 150 minutes.
삭제delete 삭제delete 삭제delete 삭제delete 상기 제1항에 따른 쌀을 이용한 맥주 제조방법에 의하여 제조되는 맥주.Beer produced by the method for producing beer using rice according to claim 1.
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