KR102371112B1 - Anti-oxidant and Anti-obesity composition comprising Complex the extract of Allium senescens.L and crisium setidens as the effient composition - Google Patents

Abstract

The present invention provides a composition for preventing and treating or preventing and improving obesity, comprising a mixture (complex) formulated in an equal ratio (w/w) of 1:1 of the dried powder of Dume leek extract and Gondre extract, The mixture mixed with the same ratio (w/w) of the dry powder of Dume leek and Gondre extracts is better than Dume leek single extract or Gondre single extract due to the synergistic effect of Dume leek extract and Gondre extract when treated at the same concentration as the single extract. There is an outstanding effect of remarkable anti-obesity.

Description

Anti-oxidant and Anti-obesity composition comprising Complex the extract of Allium senescens.L and crisium setidens as the effient composition

The present invention relates to an anti-obesity composition and a method for preparing the same, and more particularly, to an extract mixture (complex) of Dume leek and gondre, a pharmaceutical composition for preventing and treating obesity containing the same as an active ingredient, and health function for preventing and improving obesity It relates to food and a quasi-drug composition for preventing and improving obesity.

Dume leek ( Allium senescens L. ) is a perennial herb belonging to the family Liliaceae , and it was named because it grows in rare places such as deep mountains. It is a plant that grows widely in Europe and Asia, as well as in the mountainous regions of Gangwon-do, Ulleungdo, and Baekdusan, as well as in Europe and Asia. In Donguibogam, it is said about Dume leek, "It is warm in nature, has a spicy taste, and is non-toxic. It is good for the stomach and gives energy to those who have weak energy."." is introduced. The main active ingredients contained in Dume leek are known as allicin and allin , which are contained in large amounts in common Allium genus plants, and their physiological activities regarding antibacterial effects, liver health improvement, and antioxidant activity have been reported in the literature. . In addition, among plants of the genus Allium , quercetol is the most abundant ingredient in Dume leek, which contains about 993.90g/100g of living things. Quercetol is a component that has been commercialized as a health supplement in Colombia, and its physiological activities such as anticancer effect and detoxification effect from lead poisoning have been reported. Choi et al. (2014) reported a study on the content that the methanol extract of Dume leek inhibited the generation of reactive oxygen species in differentiation-induced adipocytes and suppressed the expression of adipocyte differentiation factors.

Gondree, also known as Goryeo thistle ( Cirsium setidens ), is a perennial herb belonging to the Compositae family. am. Gondree is a material with high expectations for physiological activity due to its high fiber content and polyphenol content, and a lot of research has been done recently. Anti-oxidation, liver protection from alcoholic and non-alcoholic damage, and anti-obesity effects have been reported, and pectolinarin and its non-glycoside pectolinarigenin are known as indicator or active ingredients. Noh et al. (2013) reported that Gondree extract inhibited the accumulation of fat in the liver by promoting beta oxidation in rats fed a high-fat diet, and confirmed its potential as a composition for preventing, treating or improving obesity. Cho (2017) et al. reported that in the process of confirming the active ingredient (pectolinarin) content and fat accumulation inhibitory effect of Gondree at each harvest period, the ethanol extract of Gondae affected transcription factors related to fat accumulation, leading to differentiation-induced fat in adipocytes. It was found that the production was effectively inhibited. As a measure to improve the bioavailability of pectolinarin, which is known as an active ingredient of Gondae, in a study on the deglycosylation of Gondae through bioconversion, the Gondree extract bioconverted through the fermentation process suppressed higher fat accumulation than the Gondree extract before fermentation. showed efficacy. It is estimated that this result indicates that the bioavailability of pectolinarigenin, which is a non-glycoside form of pectolinarin, is high.

Modern people often experience complications due to overweight and obesity due to lifestyle habits that reduce activity as food becomes abundant and life structure becomes more convenient. is expected to have a detrimental effect on society as a whole as it has a fatal effect on physical and mental health. The World Health Organization (WHO) warns that industrialization worldwide will become more common as industrialization becomes more common and food resources become plentiful. seems to have an impact.

Various natural resources contain a large amount of polyphenols such as flavonoids and carotenoids and are known for their antioxidant action, and the anti-obesity efficacy of extracts extracted from Dume leek and Gondree has also been reported through literature. In addition, the mechanism of action of the fermented gondree extract to inhibit the differentiation of adipocytes has also been known. However, when mixed with Doume leek extract, which significantly inhibits adipocyte differentiation, and Gondree extract to effectively decompose fat accumulated in fat through the promotion of beta oxidation of unsaturated fatty acids, a combination of different mechanisms of action has a synergistic effect. There has been no known literature disclosed or implied so far.

Accordingly, the present inventors demonstrated the synergistic effect of the complex of Dume leek and gondre extracts compared to the single extracts of each raw material by confirming the effect of inhibiting the differentiation of adipocytes and the accumulation of fat within the range that does not show cytotoxicity. The invention was completed.

1: Patent No. 10-1766911 (2017-08-03, registered) "Method for producing fermented gondree for inhibition of adipocyte differentiation through bioconversion"

1: Marcel P. et al. (2011) Allicin and allin content and antifungal activity of Allium senescens L. ssp montanum (F.W.Schmidt) Holub ethanol extract. Journal of Medicinal Plant Research 5(29):6544-6549. 2: Shin GM et al. (2018) Anti-hepatofibrosis effect of Allium senescens in activated hepatic stellate cells and thiacetamide-induced fibrosis rat model. Pharmaceutical Biology 56(1):632-642. 3: Choi HY, and Kim GH. (2014) Inhibitory effects of Allium senescens L. methanol extrats on reactive oxygen species production and lipid accumulation during differentiatioin in 3T3-L1 cells. Korean Journal of Food Science and Technology 46(4):498-504. 4: Kim EH, and Chung J. (2016) Protective effects of Cirsium setides ethanolic extracts against alcoholic fatty liver injury in rats. Journal of Nutrition and Health 49(6):420-428. 5: Cho BY et al. (2017) Changes in antioxidant and antiobesity activities of Cirsium setidens nakai ethanolic extract depending on different harvest time. Journal of Food Hygiene and Safety 32(3):234-242. 6: Vlase L et al. (2013) Chemical constituents of three Allium species from romania. Molecules 18:114-127. 7: Ali H et al. (2016) Isolation and evaluation of biological efficacy of quercetol in human hepatic carcinoma cells. Drug Design, Development and Therapy 6(10):155-162. 8: Taralunga G et al. (2010) In vivo quercetol effect in lead acetate poisoning. Farmacia 58(1):54-61

It is an object of the present invention to confirm the synergistic effect of a mixture (complex) of Dume leek and Gondree extract compared to a single extract on the anti-obesity effect.

Another object of the present invention is to provide a pharmaceutical composition for preventing or treating obesity, comprising a mixture (hereinafter, referred to as an extract mixture) of Dume leek and gondre extract.

Another object of the present invention is to provide a health functional food for the prevention or improvement of obesity comprising a mixture of Dume leek and Gondree extract.

Furthermore, it is another object of the present invention to provide a quasi-drug composition for preventing or improving obesity comprising a mixture of Doume leek and Gondree extract.

The object of the present invention is

(a) drying Dume leek and Gondree, respectively, and pulverizing each to obtain a raw material powder;

(b) mixing each raw material powder obtained in the above step with a solvent (water or ethanol), stirring and extracting at room temperature, and then concentrating and drying each;

(c) uniformly mixing each extract obtained in step (b) at a predetermined ratio;

(d) Evaluating and confirming the anti-obesity synergistic effect of a mixture (complex) obtained by mixing Dume leek and Gondre extracts obtained in step (c) in a certain ratio compared to the single extract obtained in step (b) It is achieved through the step of confirming .

Dume leek can be selected from all species classified as Allium senescens and is a perennial herb in the family Liliaceae. Dume leek of the present invention may refer to some or all of various parts, that is, leaves, stems, fruits, flowers, seeds, and roots, and specifically may refer to the leaves and stem parts.

In one embodiment of the present invention, the gondre or Korean thistle may be selected from all species classified as Cirsium setidens , and is a perennial herb of the Asteraceae family. In the present invention, gondre may refer to some or all of various parts, ie, leaves, stems, fruits, flowers, seeds, and roots, and specifically may refer to a leaf part.

As used herein, the term "extract" refers to a filtrate obtained after drying and filtering raw materials by appropriately mixing water or alcohol with a dry powder raw material obtained by drying and pulverizing raw materials, extracting them by general extraction methods such as stirring, ultrasonication, shaking, reflux, etc. Although not limited thereto, it may be an extract obtained by extraction treatment, a diluted or concentrated liquid of the extract, a dried product obtained by drying the extract, or a crude product or purified product thereof. With respect to the extract, it can be prepared and used alone or by mixing specific fractions by using an additional separation and purification method.

As used herein, the term "dume leek and gondre extract mixture (complex)" means that the extract obtained by extracting the doume leek and gondre raw materials respectively is mixed in an arbitrary ratio, and the mixing ratio is 99.9999:0.0001 to 0.0001:99.9999 by weight It can be set and used within the sub range. The manufacturing order of the extract complex is not limited to the above-mentioned method, and a method in which the two raw materials are first mixed and then subjected to an extraction process can be used, and it does not matter whether the biological raw material is mixed from the drying process before proceeding.

The Dume leek and Gondre extract complex may be an extract obtained by extracting the washed and dried Dume leek or Gondre powder with water, a lower alcohol having 1 to 6 carbon atoms, or a mixed solvent thereof, specifically, 10 to 70 parts by volume of a lower alcohol may be an extract extracted with a solvent mixed with 90 to 30 parts by volume of water, and more specifically, may be an extract extracted with a solvent obtained by mixing 30 parts by volume of ethanol and 70 parts by volume of water. In addition, the extraction may be carried out at a temperature of 4 to 85 ℃, specifically, it may be carried out at 25 ℃. The extraction may be repeated several times, and the extracted sample may be obtained by filtration, concentration and drying with a vacuum concentrator.

In the present invention, the antioxidant effect can be achieved by identifying the total polyphenol content and DPPH radical scavenging ability, which are widely known for their antioxidant activity, and according to a specific embodiment of the present invention, the total polyphenol content is found to be high. It was confirmed (FIG. 1), and according to another specific example, it was confirmed that the Doumebu extract and Gondree extract mixture (complex) according to the present invention exhibited a remarkable DPPH radical scavenging ability compared to each single material of the Doumebu extract and Gondre extract. It was also confirmed that there is a synergistic effect of efficacy (Fig. 2).

In the present invention, safety can be achieved by showing a survival rate of 90% or more through MTT assay using pre-adipocytes, and according to a specific embodiment of the present invention, each 50 , at concentrations of 100 and 200 ug/mL, all showed more than 90% viability, confirming the absence of cytotoxicity (Figure 3).

In the present invention, the prevention or treatment of obesity can be achieved by inhibiting the differentiation of pre-adipocytes into adipocytes, and according to a specific embodiment of the present invention, the mixture of extracts from D. By inhibiting it, the fat accumulation inhibitory effect was confirmed, and in particular, it was confirmed that there was a synergistic effect by showing a more pronounced activity than when the single extract of Dume leek or the single extract of Gondree was used (Fig. 4).

The pharmaceutical or food composition for preventing, treating, and improving obesity of the present invention can be used singly, and can be prepared and used as a combination formulation by additionally including another composition for which the efficacy of preventing or treating obesity is recognized, and is allowed according to the use Formulations may be made with the addition of possible carriers, excipients, or diluents.

The composition may be in various formulations, such as oral or parenteral, and when formulated, commonly used fillers, extenders, binders, wetting agents, disintegrants, and diluents or excipients such as surfactants may be used. Solid preparations for oral administration include tablets, pills, powders, granules, capsules, etc., and these solid preparations include one or more excipients, for example, dextrin, maltodextrin, starch, calcium carbonate, sucrose or lactose ( It can be prepared by mixing lactose), gelatin, etc. In addition, a lubricant such as magnesium stearate or talc may be used. Liquid formulations for oral administration include suspensions, internal solutions, emulsions, syrups, etc., and various excipients, such as wetting agents, sweeteners, fragrances, and preservatives, in addition to water and liquid paraffin, which are commonly used simple diluents, are included. can Formulations for parenteral administration include sterile aqueous solutions, non-aqueous solutions, suspensions, emulsions, freeze-dried preparations, and suppositories. Non-aqueous solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. As the base of the suppository, witepsol, macrogol, tween 61, cacao butter, laurin, glycerogelatin, etc. can be used.

In the composition of the present invention, the Dumebuchu gondre extract mixture may be included in a pharmaceutically effective amount. In the present invention, the term "pharmaceutically effective amount" means an amount sufficient to treat a disease at a reasonable benefit/risk ratio applicable for the purpose of medical treatment, and the effective dose level is determined by the type and severity of the subject, age, sex, drug activity, sensitivity to drugs, administration time, administration route and excretion rate, duration of treatment, factors including concurrent use, and other factors well-known in the medical field. The composition of the present invention may be administered as an individual therapeutic agent or in combination with other therapeutic agents, and may be administered sequentially or simultaneously with conventional therapeutic agents. and may be administered single or multiple. Taking all of the above factors into consideration, it is important to administer an amount that can obtain the maximum effect with a minimum amount without side effects, and can be easily determined by those skilled in the art.

The pharmaceutical composition of the present invention can be administered in a pharmaceutically effective amount. As used herein, the term "administration" means introducing a predetermined substance to an individual by an appropriate method, and the administration route of the composition may be administered through any general route as long as it can reach a target tissue. Intraperitoneal administration, intravenous administration, intramuscular administration, subcutaneous administration, intradermal administration, oral administration, topical administration, intranasal administration, intrapulmonary administration, may be administered intrarectally, but is not limited thereto. The term “individual” refers to all animals, including humans, rats, mice, and livestock that have or can develop obesity.

As another aspect, the present invention provides a health functional food for preventing or improving obesity comprising a mixture of Doume leek and gondre extract.

The term "health functional food" as used herein refers to a food manufactured and processed by extracting, concentrating, refining, mixing, etc., a specific ingredient as a raw material or a specific ingredient contained in a food raw material for the purpose of health supplementation, and the ingredient It refers to food designed and processed to sufficiently exert biological control functions, such as biological defense, regulation of biological rhythm, prevention and recovery of disease, etc. related functions can be performed.

In addition, there is no limitation on the type of health functional food in which the composition of the present invention can be used. In addition, the composition comprising the complex of the present invention as an active ingredient can be prepared by mixing an appropriate other auxiliary ingredient that can be contained in a health functional food and a known additive according to the selection of those skilled in the art. Examples of foods that can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages and There are vitamin complexes and the like, and it can be prepared by adding the complex according to the present invention as a main component to juice, tea, jelly, juice, and the like.

As another aspect, the present invention provides a quasi-drug composition for preventing or improving obesity comprising a mixture of Dume leek and Gondrei extract.

In the present invention, the term "quasi-drug" refers to fibers, rubber products, or the like, which are used for the purpose of treating, alleviating, treating or preventing diseases of humans or animals, have a weak action on the human body or do not directly act on the human body, and Non-machines and similar products, which fall under one of sterilization, insecticide, and similar preparations used for the urethra for the prevention of infection type, used for the purpose of diagnosing, treating, alleviating, treating or preventing diseases of humans or animals It refers to items that are not instruments, machines or devices, and items that are not used for the purpose of pharmacologically affecting the structure and function of humans or animals, other than those that are not instruments, machines, or devices.

When the composition of the present invention is used as a quasi-drug additive, the complex may be added as it is or used together with other quasi-drugs or quasi-drug ingredients, and may be appropriately used according to a conventional method. The mixing amount of the active ingredient may be suitably determined according to the purpose of use.

The quasi-drug composition of the present invention is not limited thereto, but specifically, it may be a disinfectant cleaner, shower foam, mouthwash such as Gagreen, wet tissue, detergent soap, hand wash, humidifier filler, mask, ointment, patch or filter filler.

The composition comprising the extract of Dume leek and Gondree extract of the present invention as an active ingredient can be usefully used as an anti-obesity composition by very effectively inhibiting the differentiation of pre-adipocytes into adipocytes, so it may occur due to the accumulation of fat. It can be used as a pharmaceutical composition for the prevention or treatment of fatty liver, fatty fibrosis, cardiovascular disease, a health functional food for preventing or improving obesity, or a composition for quasi-drugs.

1 is a graph showing the polyphenol content of a doume leek extract, a gondre extract, and a complex prepared by mixing a doume leek extract and a gondre extract in equal ratios according to an embodiment of the present invention.
2 is a graph showing the results of a DPPH radical scavenging ability test of a mixture of Doume leek extract, Gondre extract, and Doume leek and Gondre extract prepared according to an embodiment of the present invention in equal ratio.
FIG. 3 is a diagram showing the cytotoxicity results on pre-adipocytes (3T3-L1) of Dume leek extract, Gondree extract, and a mixture of Dume leek and Gondre extract prepared according to an embodiment of the present invention in equal ratios. .
4 is a process for adipocytes by treating differentiation-induced preadipocytes (3T3-L1) with a mixture of Doume leek extract, Gondree extract, and Doume leek and Gondre extract prepared according to an embodiment of the present invention in equal ratios; Figure showing the efficacy of inhibiting the differentiation of

Hereinafter, the present invention will be described in more detail through specific examples. These examples are for illustrative purposes, and the scope of the present invention is not limited to these examples.

Example 1: Preparation of Dume leek and Gondre extract mixture

Dume leek and gondree creatures were purchased, dried in the sun, and then the dried raw materials were pulverized. 30% ethanol was added to each dry powder raw material 20 times (w/w) with respect to the raw material, followed by extraction with stirring at room temperature. The extracts of each raw material are concentrated under reduced pressure, freeze-dried, and then pulverized (referred to as 'Dume leek extract' and 'Gondre extract', respectively), and each extract obtained in this way is uniformly mixed at a ratio of 1:1 (w/w) Dume leek and gondre extract mixture (complex) was used as a sample.

Example 2: Measurement of total polyphenol content of Dume leek and gondre extract mixture

The total polyphenol content of the Dumebu extract and the Gondre extract prepared in the above example and each of these extract mixtures (1:1, w/w) was carried out by modifying Gutfinger's Folin-Denis method. Add 1 mL of 50% folin-ciocalteu's reagent to 1 mL of a uniformly diluted sample, mix, and leave for 3 minutes. Then, add 1 mL of 10% Na ₂ CO ₃ and shake, and leave at room temperature for 1 hour using a spectrophotometer. to measure the absorbance at 700 nm. The content (mg/g) was converted by measuring gallic acid prepared in various concentrations in the same way as a standard product and substituting it for the proportional formula of the standard curve. The total polyphenol content of each sample was found to be 20.2 to 2.58 mg/g for Dumebu extract, 65.8 to 1.07 mg/g for Gondre extract, and 46.2 to 1.31 mg/g for Dumebuchu and Gondre extract mixture.

Example 3: Measurement of DPPH radical scavenging activity of a mixture of Dume leek and gondre extract dry powder

The DPPH radical scavenging activity for the Dumebu extract and Gondre extract and their respective extract mixtures (1:1, w/w) prepared in Example 1 was measured by applying the method of Brand-Williams et al. That is, the DPPH solution was prepared by dissolving DPPH (2,2-Diphenyl-1-picrylhydrazyl) in 50% ethanol at a concentration of 150 uM. After that, 500uL of the DPPH solution and 500uL of each sample diluted to a constant concentration were mixed, reacted at room temperature for 10 minutes, and absorbance was measured at 517 nm. As shown in [Fig. 2], the DPPH radical scavenging activity of the Dumebu extract was 22.2% and that of the Gondae extract was 43.5%, and their mixture showed a radical scavenging activity of 51.0%, which showed that the antioxidant activity compared to the single extract was 129.9%, respectively. , increased by 17.3%. Based on this, it was confirmed that the extract mixture of the Dumebu extract and the Gondre extract of the present invention has a synergistic effect on antioxidant activity compared to a single material.

Example 4: Cytotoxicity analysis of Dume leek and Gondre extract mixture

In order to confirm the cytotoxicity of the Dumebu extract and Gondae extract prepared in Example 1 and each extract mixture (1:1, w/w), the mouse precursor cell line 3T3-L1 (American Type Culture Collection; ATCC) , USA) was measured by applying the MTT assay. Specifically, the MTT solution was dissolved in PBS (phosphate buffered saline) to 5 mg/mL, filtered through a syringe filter, packaged in aluminum foil, and stored in a refrigerator. Cells were aliquoted in a 24-well plate so that the number of cells was 2 x 10 ⁴ cells/well and incubated for 24 hours. treated with and incubated for 24 hours. After that, 500uL per well was washed twice with SF, 20uL of MTT solution was dispensed, and then cultured at 37°C for 4 hours. After removing the medium, 100uL of DMSO was dispensed. After wrapping it with foil and leaving it at room temperature for 30 minutes, 80uL was taken and transferred to a 96-well plate, and absorbance was measured at a wavelength of 570 nm. Based on the untreated group (CON), it was determined that there was no cytotoxicity in samples and concentrations showing a survival rate of 90% or more, and it was confirmed that there was no cytotoxicity in all samples and concentrations [Fig. 3].

Example 5: Analysis of adipocyte differentiation inhibitory ability of Dume leek and gondre extract mixture (1:1, w/w)

The ability to inhibit adipocyte differentiation of the Dumebu extract and Gondae extract prepared in Example 1 and each extract mixture (1:1, w/w) was tested by artificially inducing differentiation in 3T3-L1, a mouse precursor cell line. Identified by processing the material. Specifically, 3T3-L1 progenitor cells were cultured in DMEM (Dulbeco's Modified Eagles' Media) medium supplemented with 10% bovine calf serum (BCS) and 1% penicillin/streptomycin. After dispensing the cells in a 24-well plate so that the cells were 2 x 10 ⁴ cells/well, the cells were cultured to a confluent state. Differentiation induction was performed as follows for each cycle. The initial induction of differentiation was performed by mixing 10% fetal bovine serum (FBS), 1uM dexamethasone, 0.5mM 3-isobutyl-1-methylxanthine (IBMX), and 5ug/mL insulin in DMEM medium for 2 days. cultured. Next, incubation was performed for an additional 2 days using a medium containing 10% FBS and 5ug/mL insulin in DMEM medium. Finally, the medium containing 10% FBS in DMEM medium was changed every 2 days, 4 It was maintained for ~8 days. In all differentiation induction media, each test substance was treated at each concentration (50, 100, 200 ug/mL). After differentiation induction was completed, the cell culture medium was removed, washed with PBS, and then 500 uL of 4% paraformaldehyde was added, and the cells were left standing for at least 1 hour to fix the cells. After fixation was completed, paraformaldehyde was removed, washed with 60% isopropanol, treated with 500uL of Oil Red O staining solution, and left for 1-2 hours for staining. The dyeing reagent was used by mixing Oil Red O (0.5% in isopropanol) reagent and distilled water in a ratio of 3:2 (v/v). The results were derived by washing the cells with distilled water 4 times, lysing the cells with isopropanol, and measuring the absorbance at a wavelength of 495 nm. The results were expressed as a percentage of the absorbance reduced based on the control group (CON) that was not treated with anything, and is shown in FIG. 4 . When the concentration of the test substance was confirmed based on 100ug/mL, it was confirmed that the Dumebu extract and Gondree extract were reduced by 19.3% and 7.5%, respectively, compared to the untreated group, CON, and 39.4% in the Dumebuchu gondre extract mixture, which was mixed in the same ratio. % reduction, and it was found that the synergistic effect was very clear compared to the single material. This explains that the mixture exhibits excellent synergistic effect by showing 104.1% and 425.3% higher body fat accumulation inhibitory effects, respectively, compared to Dumebu extract and Gondre extract alone.

Claims (5)

To each dry powder raw material of Dume leek and Gondree, 20 times (w/w) of 30% ethanol was added, stirred and extracted at a temperature of 4 to 85°C, concentrated under reduced pressure, freeze-dried, and pulverized to 1:1 ( w/w) A method for producing a mixture of Doume leek and Gondre extract, characterized in that it is blended in a ratio. A pharmaceutical composition for the prevention or treatment of obesity disease, comprising as an active ingredient a mixture of Dume leek and gondre extract prepared by the method of claim 1 . A health functional food composition for preventing or improving obesity, comprising as an active ingredient a mixture of Doume leek and Gondre extract prepared by the method of claim 1. A quasi-drug composition for preventing or improving obesity, comprising as an active ingredient a mixture of Doume leek and Gondre extract prepared by the method of claim 1. [Claim 5] The quasi-drug composition according to claim 4, wherein the quasi-drug composition is one selected from disinfectant cleaner, shower foam, mouthwash, wet tissue, detergent soap, hand wash, humidifier filler, mask, ointment, patch and filter filler. composition.

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