KR102370859B1 - Composition for controlling pine wood nematode containing saccharine - Google Patents
Composition for controlling pine wood nematode containing saccharine Download PDFInfo
- Publication number
- KR102370859B1 KR102370859B1 KR1020200044560A KR20200044560A KR102370859B1 KR 102370859 B1 KR102370859 B1 KR 102370859B1 KR 1020200044560 A KR1020200044560 A KR 1020200044560A KR 20200044560 A KR20200044560 A KR 20200044560A KR 102370859 B1 KR102370859 B1 KR 102370859B1
- Authority
- KR
- South Korea
- Prior art keywords
- composition
- pine wilt
- pine
- nematodes
- controlling
- Prior art date
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/80—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/34—Shaped forms, e.g. sheets, not provided for in any other sub-group of this main group
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
- Zoology (AREA)
- Toxicology (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
본 발명은 사카린을 포함하는 소나무재선충 방제용 조성물 및 이를 이용한 소나무재선충을 방제하는 방법에 관한 것이다.The present invention relates to a composition for controlling pine wilt nematodes containing saccharin and a method for controlling pine wilt nematodes using the same.
Description
본 발명은 사카린을 포함하는 소나무재선충 방제용 조성물 및 이를 이용한 소나무재선충을 방제하는 방법에 관한 것이다.The present invention relates to a composition for controlling pine wilt nematodes containing saccharin and a method for controlling pine wilt nematodes using the same.
소나무재선충(Bursaphelenchus xylophilus)은 소나무재선충병을 일으켜 소나무와 잣나무에 심각한 피해를 입히고 있다. 이 식물병은 한번 감염되면 치료가 되지 않고 거의 모든 소나무를 고사시킨다. 우리나라의 경우, 남부지방에서는 주로 소나무가 피해를 입고 있고, 중부지방에서는 잣나무가 주로 피해를 입고 있다. 소나무재선충(Bursaphelenchus xylophilus)은 분류학상으로 후생동물아계(Metazoa), 진정후생동물(Eumetazoa), 좌우대칭동물(Bilateria), 의체강동물(Pseudocoelomata), 선충문(Nematoda, Nemata)에 속하는 동물로서 실모양을 하고 있다. 길이는 1㎜도 안되지만, 번식력이 높아 한 쌍이 20일 만에 20만 마리로 늘어날 정도이다. 소나무재선충은 스스로 나무에 침입하거나 나무 사이를 이동할 수 없고, 반드시 매개충을 통해야만 가능하다. 매개충인 솔수염하늘소(Monochamus alternatus) 또는 북방수염하늘소(M. saltuarius)는 주로 병든 소나무나 잣나무를 선택해 알을 낳는데, 재선충에 감염된 나무에 낳은 매개충의 알이 봄에 번데기로 될 때 재선충이 주위에 모이게 되고, 매개충이 5월 중순 ~ 7월 하순 동안 우화(羽化)할 때 몸에 붙어 있는 재선충도 함께 이동한다. 목질부를 탈출한 매개충은 건강한 나무의 새로 자란 가지(1~2년생 가지)를 주로 갉아먹으며, 이때 생긴 상처를 통하여 소나무재선충을 감염시킨다. 병든 소나무의 경우, 잎 전체가 갈색으로 변하면서 보통 1년 안에 말라죽고 만다. 1905년 일본에서 처음으로 발병한 소나무재선충병의 원인은 오랜 세월 규명되지 않다가 1970년에 이르러 비로소 원인이 밝혀졌다. 일본은 이 병에 의해 역사적 가치가 높은 조경수를 제외하고는 대부분의 소나무 자생림이 사라졌다. 우리나라의 경우에는 1988년 부산 금정산에서 처음으로 발생하였는데, 초기에는 항공 살포에 의한 매개충 박멸, 감염목의 조기 발견 및 제거, 감염목 주변나무들의 적극적인 수간 주입 등으로 부산 일대에서만 발병할 뿐 잠잠했다. 하지만, 살충제의 항공 살포에 의한 꿀벌 등의 유익충에 대한 피해, 환경오염 등의 이유로 인한 환경단체 중심의 항의 시위 및 국민들의 피해목 이동 등으로 효과적인 방제가 진행되지 못하였고, 결국에는 전국에 걸쳐서 소나무재선충병이 발병하게 되었다.The pine wilt nematode (Bursaphelenchus xylophilus) causes pine ash nematode disease, causing serious damage to pine and cypress trees. This plant disease, once infected, is not curable and kills almost all pine trees. In the case of Korea, pine trees are mainly damaged in the southern regions, and pine trees are mainly damaged in the central regions. The pine wilt nematode (Bursaphelenchus xylophilus) is taxonomically classified as an animal belonging to the subfamily Metazoa, Eumetazoa, Bilateria, Pseudocoelomata, and Nematoda, Nemata. is taking shape Although the length is less than 1 mm, the fertility is so high that a pair can grow to 200,000 in 20 days. Pine wilt nematodes cannot invade trees or move between trees by themselves, but only through insect vectors. The insect vectors Monochamus alternatus or M. saltuarius mainly select diseased pines or cypress trees to lay eggs. The nematodes that are attached to the body migrate together when the insect vectors emerge from mid-May to late July. The vector that escaped from the xylem mainly gnaws at the newly grown branches (1~2 year old branches) of healthy trees, and infects the pine wilt nematodes through the wounds. In the case of diseased pines, the entire leaf turns brown and usually dies within a year. The cause of pine wilt disease, which first appeared in Japan in 1905, has not been identified for a long time, but the cause was only revealed in 1970. In Japan, most of the native pine forests except for landscape trees with high historical value disappeared due to this disease. In Korea, the first outbreak occurred in Mt. Geumjeong, Busan in 1988. In the early days, the disease was quiet only in the Busan area due to the eradication of insect vectors by aerial spraying, early detection and removal of infected trees, and active stem injection of trees around the infected trees. However, effective control was not carried out due to damage to beneficial insects such as bees caused by aerial spraying of pesticides, protests by environmental groups centered on environmental pollution, and people's movement of damaged trees. A pine wilt disease has occurred.
소나무재선충은 소나무속(Pinus) 뿐만 아니라, 전나무속(Abies), 가문비나무속(Picea), 잎갈나무속(Larix)의 일부 수종 등도 가해하는 것으로 알려져 있다. 경상도, 전라도 및 강릉 지역은 주로 소나무에서 발병되는 반면, 강원도 서부 지역과 경기도에서는 주로 잣나무에서 발병이 되었다. 남부지방과 중부지방의 수목 피해종의 이러한 차이는 소나무재선충을 매개하는 매개충의 차이에 의한 것으로 알려져 있다. 남부지방의 경우, 솔수염하늘소의 밀도가 높고, 중부지방의 경우 북방수염하늘소의 밀도가 높은데, 이들 매개충이 선호하는 식물체가 서로 다르기 때문인 것으로 추정되고 있다.The pine wilt nematode is known to injure not only the genus Pinus, but also some species of the genus Abies, spruce (Picea), and larch (Larix). In Gyeongsang-do, Jeolla-do, and Gangneung regions, the disease mainly occurred in pine trees, whereas in the western part of Gangwon-do and Gyeonggi-do, the disease mainly occurred in pine trees. It is known that this difference in tree-damaged species in the southern and central regions is due to the difference in the vector of pine wilt nematodes. In the southern region, the density of Monochamus alternatus is high, and in the central region, the density of Monochamus alternatus is high.
이 식물병을 방제하기 위한 방법으로는, 1) 건전한 나무에 살선충약제의 예방적인 수간 주입, 2) 매개충 방제를 위한 합성 살충제 또는 길항 미생물의 항공 살포, 3) 감염목으로부터 재선충과 매개충을 방제하기 위한 감염목의 파쇄, 연소 및 훈증 등의 방법이 있다. 이 중, 감염목의 파쇄, 연소 및 훈증 방법은 사후적 방제방법으로서, 이미 재선충에 감염된 수목을 처리하는 방법일 뿐 소나무재선충병을 예방하거나, 감염된 소나무를 치료하고 소생시키는 방법이 아니라는데 근원적 문제가 있다. 직접적으로 소나무재선충을 타겟으로 하는 방제법은 살아있는 소나무에 예방약을 수관 주입하는 방법밖에 없다.Methods for controlling this plant disease include: 1) prophylactic injection of nematodes into healthy trees, 2) aerial spraying of synthetic insecticides or antagonistic microorganisms to control insect vectors, 3) control of wilt nematodes and vectors from infected trees There are methods such as crushing, burning, and fumigation of infected trees. Among them, crushing, burning and fumigation of infected trees is a post-mortem control method, and it is a method of treating trees already infected with wilt nematodes, not preventing pine wilt disease or treating and revitalizing infected pine trees. there is The only control method that directly targets the pine wilt nematode is to inject a preventive agent into the living pine tree.
본 발명자들은 소나무재선충을 타겟으로 하는 방제법을 연구하던 중에 인공감미료 중에 하나인 사카린이 소나무재선충에 처리한 결과, 사카린의 농도 의존적으로 소나무재선충의 치사율이 증가하는 것을 확인하고 본 발명을 완성하였다. The present inventors have completed the present invention by confirming that saccharin, one of the artificial sweeteners, was treated on pine wilt nematodes while researching a control method targeting pine wilt nematodes, and confirmed that the mortality rate of pine wilt nematodes increased in a concentration-dependent manner of saccharin.
본 발명자들은 소나무재선충을 타겟으로 하는 방제법을 연구하던 중에 인공감미료 중에 하나인 사카린이 소나무재선충에 처리한 결과, 사카린의 농도 의존적으로 소나무재선충의 치사율이 증가하는 것을 확인하고 본 발명을 완성하였다. The present inventors have completed the present invention by confirming that saccharin, one of the artificial sweeteners, was treated on pine wilt nematodes while researching a control method targeting pine wilt nematodes, and confirmed that the mortality rate of pine wilt nematodes increased in a concentration-dependent manner of saccharin.
본 발명의 목적은 사카린을 포함하는 소나무재선충 방제용 조성물을 제공하는 것이다. An object of the present invention is to provide a composition for controlling pine wilt nematodes comprising saccharin.
상기 과제를 해결하기 위해, 사카린을 포함하는 소나무재선충 방제용 조성물을 제공할 수 있다. In order to solve the above problems, it is possible to provide a composition for controlling pine wilt nematodes comprising saccharin.
상기 사카린의 농도는 2M 내지 0.1M일 수 있다. The concentration of the saccharin may be 2M to 0.1M.
상기 서술한 방제용 조성물에 살충제를 추가로 포함할 수 있다. An insecticide may be further included in the above-mentioned composition for controlling.
상기 조성물이 현탁제, 유제, 액제, 수화제, 과립수화제, 입제, 분제, 정제 및 팩제 중에서 선택되는 어느 하나의 제형을 가질 수 있다. The composition may have any one formulation selected from suspensions, emulsions, solutions, wettable powders, granular wettable powders, granules, powders, tablets, and packs.
본 발명은 또한 상기 서술한 조성물을 식물 또는 토양에 처리하여 소나무재선충을 방제하는 방법을 제공할 수 있다. The present invention may also provide a method for controlling pine wilt nematodes by treating the above-described composition on plants or soil.
본 발명에 따르면 사카린은 소나무재선충병에 농도 의존적으로 소나무재선충을 사멸시키는 효과가 있다. 사카린을 포함하는 소나무재선충병 방제를 할 수 있는 효과가 있다. According to the present invention, saccharin has an effect of killing pine wilt nematodes in a concentration-dependent manner against pine wilt disease. It is effective in controlling the pine wilt disease containing saccharin.
도 1은 사카린 수용액에 따른 살재 선충율의 결과이다.1 is a result of the nematode killing rate according to the saccharin aqueous solution.
상기 과제를 해결하기 위해, 사카린을 포함하는 소나무재선충 방제용 조성물을 제공할 수 있다. In order to solve the above problems, it is possible to provide a composition for controlling pine wilt nematodes comprising saccharin.
본 발명은 소나무재선충병의 병방제 기작을 규명하는 연구를 진행하는 중에 사카린이 기존에 연구되지 않았던 소나무재선충병의 기주인 소나무류에 저항성을 유도하는데 매우 중요한 역할을 하며, 이를 활용하여 소나무재선충병을 방제할 수 있다는 사실을 발견하였다. The present invention plays a very important role in inducing resistance to pine trees, the host of pine wilt disease, which has not been studied before, in which saccharin plays a very important role in inducing resistance to pine wilt disease, which has not been studied before, during research to identify the control mechanism of pine wilt disease. was found to be able to control
본 발명은, 사카린은 농약학적으로 허용가능한 담체 및/또는 부형제를 추가로 포함할 수 있다. 본 발명의 조성물에 포함되는 부형제는 농업 분야에서 통상적으로 사용되고 있는 것으로, 예를 들면, 규조토, 소석회 등의 산화물, 인회석 등의 인산염, 석고 등의 황산염, 클레이, 카올린, 벤토나이트, 산성백토, 석영, 실리카 등의 광물질 분말 등의 고체 담체와 충진제, 항응집제, 계면활성제, 유화제, 방부제 등을 추가하여 포함할 수 있다. 또한, 본 발명의 조성물을 식물에 처리하여 활성성분이 신속방출, 서방출, 지연된 방출할 수 있도록 당업계에서 공지된 방법을 사용하여 제형화할 수 있다. 제형화를 위해서는 통상적으로 사용하는 계면활성제, 희석제, 분산제, 보조제 등의 첨가제를 활성성분과 배합하여 수화제, 현탁제, 유제, 유탁제, 미탁제, 액제, 분산성 액제, 입상수화제, 입제, 분제, 액상수화제, 수면부상성입제, 정제 등 각종 형태로 제제화하여 사용할 수 있다.In the present invention, saccharin may further include an agrochemically acceptable carrier and/or excipient. The excipients included in the composition of the present invention are commonly used in the agricultural field, for example, oxides such as diatomaceous earth and slaked lime, phosphates such as apatite, sulfates such as gypsum, clay, kaolin, bentonite, acid clay, quartz, It may include a solid carrier such as a mineral powder such as silica, and a filler, an anti-aggregant, a surfactant, an emulsifier, a preservative, and the like. In addition, the composition of the present invention can be formulated using a method known in the art so that the active ingredient can be rapidly released, sustained release, or delayed release by treating plants. For formulation, commonly used additives such as surfactants, diluents, dispersants, and adjuvants are mixed with the active ingredient to form wettable powders, suspensions, emulsions, emulsions, mucuss, liquids, dispersible solutions, granular wettable powders, granules, powders, It can be formulated and used in various forms such as liquid wettable powder, sleep-injury granules, and tablets.
상기 방제용 조성물은 기존에 알려있는 소나무재선충 방제용 조성물인 메탐포타시움액제, 메탐쇼듐액제, 모란텔타트레이트액제, 포스티아제이트액제, 에마멕틴벤조에이트유제, 아바멕틴유제 등과 같이 병용으로 사용할 수 있다. The control composition can be used in combination with the known compositions for controlling pine wilt nematodes, such as methampotacium solution, methamshodium solution, morantel tartrate solution, postiase solution, emamectin benzoate emulsion, abamectin emulsion, etc. there is.
본 발명에 따른 소나무재선충병 방제용 조성물은 예를 들어 직접 분사가능한 용액, 분말 및 현탁액의 형태 또는 고농축 수성, 유성 또는 다른 현탁액, 분산액, 에멀젼, 유성 분산액, 페이스트, 분진, 흩뿌림 물질 또는 과립제로 제조할 수 있으나, 이에 제한되지는 않는다. 또한, 상기 소나무재선충병 방제용 조성물은 분사, 분무, 살포, 흩뿌림 또는 붓기에 의해 사용될 수 있다. 사용 형태는 의도한 목적에 의존하는데, 모든 경우에 본 발명에 따른 조성물의 분포가 가능한 한 미세하고 균일하도록 해야 한다.The composition for controlling pine wilt disease according to the present invention is, for example, in the form of directly sprayable solutions, powders and suspensions, or highly concentrated aqueous, oily or other suspensions, dispersions, emulsions, oily dispersions, pastes, dusts, scattering materials or granules. It can be manufactured, but is not limited thereto. In addition, the composition for controlling the pine wilt nematode disease may be used by spraying, spraying, spraying, scattering or pouring. The form of use depends on the intended purpose, in all cases it should be such that the distribution of the composition according to the invention is as fine and uniform as possible.
본 발명의 소나무재선충병 방제용 조성물은 다양한 형태로 제제화할 수 있다. 상기 제제는 예를 들어 용매 및/또는 담체를 첨가함으로서 제조될 수 있다. 종종, 비활성 첨가제 및 표면-활성 물질, 예를 들어 유화제 또는 분산제를 제제에 혼합한다. 적합한 표면-활성 물질은 방향족 술폰산(예를 들어 리그노술폰산, 페놀-술폰산, 나프탈렌- 및 디부틸나프탈렌술폰산), 지방산, 알킬- 및 알킬아릴술포네이트, 알킬 라우릴 에테르, 지방 알코올 술페이트의 알칼리 금속, 알카라인 토금속, 암모늄염, 술페이트화 헥사-, 헵타- 및 옥타데칸올, 지방 알코올 글리콜 에테르의 염, 술포네이트 나프탈렌 및 이의 유도체, 포름알데히드의 축합물, 나프탈렌 또는 나프탈렌술폰산, 페놀 및 포름알데히드의 축합물, 폴리옥시에틸렌옥틸 페놀 에테르, 에톡실화 이소옥틸-, 옥틸- 또는 노닐페놀, 알킬페닐 또는 트리부틸페닐 폴리글리콜 에테르, 알킬아릴폴리에테르 알코올, 이소트리데실 알코올, 지방 알코올/에틸렌옥사이드 축합물, 에톡실화 피마자유, 폴리옥시에틸렌 알킬에테르 또는 폴리옥시프로필렌, 라우릴 알코올 폴리글리콜 에테르 아세테이트, 소르비톨 에스테르, 리그닌-술파이트 폐액 또는 메틸셀룰로오스일 수 있으나, 이에 제한되지는 않는다.The composition for controlling pine wilt nematode disease of the present invention can be formulated in various forms. The formulations can be prepared, for example, by adding a solvent and/or carrier. Often, inert additives and surface-active substances such as emulsifiers or dispersants are mixed into the formulation. Suitable surface-active substances are aromatic sulfonic acids (eg lignosulfonic acid, phenol-sulfonic acid, naphthalene- and dibutylnaphthalenesulfonic acid), fatty acids, alkyl- and alkylarylsulfonates, alkyl lauryl ethers, alkalis of fatty alcohol sulfates Metals, alkaline earth metals, ammonium salts, sulfated hexa-, hepta- and octadecanols, salts of fatty alcohol glycol ethers, sulfonates naphthalene and derivatives thereof, condensates of formaldehyde, naphthalene or naphthalenesulfonic acids, phenols and formaldehyde Condensate, polyoxyethyleneoctyl phenol ether, ethoxylated isooctyl-, octyl- or nonylphenol, alkylphenyl or tributylphenyl polyglycol ether, alkylarylpolyether alcohol, isotridecyl alcohol, fatty alcohol/ethylene oxide condensate , ethoxylated castor oil, polyoxyethylene alkyl ether or polyoxypropylene, lauryl alcohol polyglycol ether acetate, sorbitol ester, lignin-sulfite waste liquor or methylcellulose.
본 발명의 소나무재선충병 방제용 조성물은 식물체 흡수 및 효과를 증진시키기 위하여 확산제 및 침투제, 또는 계면활성제와도 혼용이 가능하다.The composition for controlling pine wilt disease of the present invention can be mixed with a spreading agent and penetrant, or a surfactant to enhance plant absorption and effect.
상기 사카린의 농도는 2.0M 내지 0.1M일 수 있다. The concentration of the saccharin may be 2.0M to 0.1M.
상기 서술한 방제용 조성물에 살충제를 추가로 포함할 수 있다. An insecticide may be further included in the above-mentioned composition for controlling.
상기 조성물이 현탁제, 유제, 액제, 수화제, 과립수화제, 입제, 분제, 정제 및 팩제 중에서 선택되는 어느 하나의 제형을 가질 수 있다. The composition may have any one formulation selected from suspensions, emulsions, solutions, wettable powders, granular wettable powders, granules, powders, tablets, and packs.
본 발명은 또한 상기 서술한 조성물을 식물 또는 토양에 처리하여 소나무재선충을 방제하는 방법을 제공할 수 있다.The present invention may also provide a method for controlling pine wilt nematodes by treating the above-described composition on plants or soil.
이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples only illustrate the present invention, and the content of the present invention is not limited to the following examples.
실시예 1. 살재선충 활성 검정을 위한 해충준비 Example 1. Preparation of pests for nematode activity assay
사카린의 살선충 및 살비 활성검정을 위하여 소나무재선충을 살선충 및 살비 활성 검정 대상으로 선정하였다. 상기 살성충활성 검정에 사용된 소나무재선충은 진주지역의 소나무재선충병에 의해 고사된 곰솔에서 시료를 채취하여 베르만방법(Baermann funnel method)으로 검출하여 잿빛곰팡이병균(Botrytis cinerea)을 먹이로 하여 온도 25±1℃ 항온조건에서 증식시켜 사용하였다.For the nematicidal and acaricidal activity test of saccharin, the pine wood nematode was selected as a nematode and acaricidal activity test target. The pine wilt nematode used in the insecticidal activity assay was detected by the Baermann funnel method after collecting a sample from the pine wilt nematode in the Jinju area, and feeding the gray mold fungus (Botrytis cinerea) at a temperature of 25 It was used by propagation at ±1°C constant temperature conditions.
실험예 1. 살재선충 활성 검정Experimental Example 1. Assay for nematode activity
사카린 선충효과를 확인하기 위하여 사카린을 정제수에 2M농도로 조제한뒤 1.8, 0.9, 0.45, 0.23, 0.11, 0.05M 농도로 희석하였다. 소나무재선충을 접종하여 24시간 경과후 소나무재선충의 치사율을 확인하였다. 사카린 희석액 처리시 살선충 효과는 0.9M 농도에서도 100%의 높은 살선충율을 보였다 (도 1). In order to confirm the saccharin nematode effect, saccharin was prepared at a concentration of 2M in purified water, and then diluted to a concentration of 1.8, 0.9, 0.45, 0.23, 0.11, or 0.05M. After 24 hours of inoculation with pine wilt nematodes, the mortality rate of pine wilt nematodes was confirmed. The nematicidal effect of saccharin dilution treatment showed a high nematicidal rate of 100% even at a concentration of 0.9M ( FIG. 1 ).
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020200044560A KR102370859B1 (en) | 2020-04-13 | 2020-04-13 | Composition for controlling pine wood nematode containing saccharine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020200044560A KR102370859B1 (en) | 2020-04-13 | 2020-04-13 | Composition for controlling pine wood nematode containing saccharine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR20210126904A KR20210126904A (en) | 2021-10-21 |
| KR102370859B1 true KR102370859B1 (en) | 2022-03-07 |
Family
ID=78268897
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1020200044560A KR102370859B1 (en) | 2020-04-13 | 2020-04-13 | Composition for controlling pine wood nematode containing saccharine |
Country Status (1)
| Country | Link |
|---|---|
| KR (1) | KR102370859B1 (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005018327A1 (en) * | 2003-08-26 | 2005-03-03 | Kumiai Chemical Industry Co., Ltd. | Benzisothiazoline derivative, plant disease control agent for agricultural or horticultural use, and pest control agent for agricultural or horticultural use |
| CN104770365A (en) | 2015-03-10 | 2015-07-15 | 华南农业大学 | Application of sweetening agent to control of fruit fly pests |
| CN104814018A (en) | 2015-03-10 | 2015-08-05 | 华南农业大学 | Application of sweetening agent in control of ant pests |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3639901A1 (en) * | 1986-11-22 | 1988-06-01 | Bayer Ag | SACCHARINE SALTS FROM SUBSTITUTED AMINES |
-
2020
- 2020-04-13 KR KR1020200044560A patent/KR102370859B1/en active IP Right Grant
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005018327A1 (en) * | 2003-08-26 | 2005-03-03 | Kumiai Chemical Industry Co., Ltd. | Benzisothiazoline derivative, plant disease control agent for agricultural or horticultural use, and pest control agent for agricultural or horticultural use |
| CN104770365A (en) | 2015-03-10 | 2015-07-15 | 华南农业大学 | Application of sweetening agent to control of fruit fly pests |
| CN104814018A (en) | 2015-03-10 | 2015-08-05 | 华南农业大学 | Application of sweetening agent in control of ant pests |
Non-Patent Citations (1)
| Title |
|---|
| Chunyan Zheng et al., ‘Effect of sweeteners on the survival and behaviour of Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)’, Pest Manag Sci, Vol.72, pp.990-996, (2015.08.07.)* |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20210126904A (en) | 2021-10-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP4118948B2 (en) | Synergistic herbicide mixture containing cyclohexenone oxime ether | |
| KR20070093121A (en) | Fungicidal mixtures | |
| KR20070093123A (en) | Fungicidal mixtures | |
| EP3685668A2 (en) | Composition comprising acibenzolar-s-methyl as effective ingredient for controlling bursaphelenchus xylophilus disease and method for controlling bursaphelenchus xylophilus using same | |
| JPH07173017A (en) | Composition for preventing pines from being damaged by death and method for prevention | |
| KR102287545B1 (en) | Composition for controlling pine wood nematode containing benzyloxyalcohol | |
| KR102370859B1 (en) | Composition for controlling pine wood nematode containing saccharine | |
| US20080166437A1 (en) | Methods of reducing pests and treating gastrointestinal nematode infections | |
| KR102288958B1 (en) | Composition for controlling pine wood nematode containing naphthoquinone-based compounds | |
| CA3017543C (en) | Fungicidal composition | |
| KR20100134075A (en) | Novel pyriproxyfen compositions | |
| CN107156160A (en) | A kind of composition and method for preventing and treating oriental fruit months | |
| KR102557889B1 (en) | Composition for controlling pine wood nematode containing extract of Chelidonium majus var. asiaticum | |
| KR101987032B1 (en) | Composition for controlling pine wilt disease comprising methyl salicylate or its derivative as effective component and method for controlling pine wilt disease using the same | |
| JP2006520340A (en) | Composition for the control of plant pests | |
| KR101508163B1 (en) | Composition for controlling plant viruses comprising ellagic acid as effective ingredient | |
| BG61128B1 (en) | Fungicidal composition | |
| KR101975256B1 (en) | Composition for controlling pine wood nematode containing novel avermectin glycoside as effective component | |
| KR102099419B1 (en) | Bee-vectoring composition for controlling plant disease comprising polyethylene glycol, skim milk and useful microorganism as effective component | |
| Eden et al. | Evaluation of spring and autumn applied insecticides for the control of black beetle | |
| MX2014005631A (en) | Combinations of antifungal compounds and tea tree oil for the treatment of oomycetes plant infection. | |
| US20080311233A1 (en) | Naturally Occurring Phenolic Substances Useful as Pesticides | |
| CN109380240B (en) | Pesticide composition containing oxathiapiprolin and application thereof | |
| KR20230133039A (en) | Novel emamectin glucoside and insecticide composition containing the same | |
| KR20240066529A (en) | Production of nematicidal pinosylvin stilbenes in cell suspension culture of Pinus koraiensis by fungal elicitation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| E902 | Notification of reason for refusal | ||
| E701 | Decision to grant or registration of patent right | ||
| GRNT | Written decision to grant |