KR102364513B1 - Immunity induction device and method for mass production of larva - Google Patents

Abstract

The immunogen injection automation system of the present invention removes the injection part pressure plate and adjusts the needle to be inserted only to a part of the groove of the injection line 21 (control so that the needle does not penetrate into the larvae) It is possible to minimize the death of the larvae from wounds and shock. .
The immunogen injection automation system of the present invention tilts the transfer unit in which the injection line is formed, and vibrates the transfer unit and at the same time sprays air to move the larvae along the injection line of the transfer unit. Compared to the method of transferring the loading part to the transfer part, the transfer method is continuous, so that the work efficiency can be improved.
The immunogen injection automation system of the present invention is provided with a vibration damping unit to reduce vibration between the transfer unit and the support unit and a wheel at the lower end of the support unit to increase mobility and work space efficiency.

Description

Immunogen injection automation system and method for mass production {Immunity induction device and method for mass production of larva}

The present invention relates to an automated immunogen injection system and method for mass production, and more particularly, the silkworm during transport through the scanning line of the inclined transfer part where the larvae are located, the inclined injection part, the vibrating part for moving the larvae and the air injection part It relates to an automated immunogen injection system and method that reduces wounds and improves transport efficiency.

Animal antibiotics have been used for growth promotion and treatment, and have played a leading role in today's large-scale intensive and commercial livestock breeding. Antibiotics for growth promotion are antibiotics that are added to feed at a low level for the purpose of promoting the growth of livestock, suppressing harmful intestinal bacteria, preventing diseases, and improving the breeding environment. In addition, therapeutic antibiotics are antibiotics administered at a high level under a veterinarian's prescription for the purpose of treating diseases when abnormal symptoms and diseases of livestock occur.

However, recently, problems such as emergence of resistant bacteria due to misuse of antibiotics in livestock feed, antibiotic residues in livestock products, weakening of disease resistance of livestock, and contamination of the ecosystem due to antibiotic residues spilled into manure are emerging.

Therefore, it is urgent to prepare countermeasures to prevent the misuse of antibiotics, to promote the hygiene and safety of livestock products by consumers, to strengthen regulations on the use of antibiotics to enhance domestic livestock competitiveness, and to promote non-antibiotic livestock product certification policies.

Recently, the development of natural antibiotics with a new mechanism of action that can combat resistant strains due to the misuse of antibiotics is being sought. Insects such as silkworms have strong antibacterial substances for self-defense through a long evolutionary history in a poor environment, and are considered to be very excellent materials for developing natural antibiotics that can replace chemical antibiotics.

According to the prior art, Korean Patent Application Laid-Open No. 2008-0083111 (transgenic silkworm producing antibody and method for producing the same), a method for producing a recombinant antibody from silk glands of the transgenic silkworm is disclosed. However, the development of a device for inducing a large amount of insects with natural antibiotic peptides was insufficient in Korea Patent Publication.

In order to improve this, Korean Patent No. 10-1896842 of the present applicant includes an input part, a loading part, an injection part, a transfer part, a collection part, and a control part, but a plurality of needles are formed in the injection part to stab the larvae An automatic injector for mass production of crevice-forming larvae is disclosed.

In the Korean patent registration, the larvae are pressed with a pressing plate and then the larvae are stabbed with a needle, and then the injection unit is raised to lift the larvae. However, in the above registered patent, the larvae were pressed with the injection presser plate (to lift the larvae with the needle) or the needle penetrated the body of the larvae, resulting in injuries and shock deaths.

In addition, since the registered patent undergoes discontinuous processes such as raising and lowering of the injection unit, movement of the loading unit through the transfer unit, and immersion in the immune inducing solution in a state in which the larvae are inserted into the needle, there is a limit to increasing the working efficiency.

The present invention provides an automated immunogen injection system and method that minimizes the death of larvae from wounds and shock.

An object of the present invention is to provide an automated immunogen injection system and method capable of improving work efficiency by reducing discontinuous processes.

One aspect of the present invention is

The input unit 10 for separating and discharging the agglomerated larvae;

a transfer unit 20 positioned at the rear end of the input unit, the scan lines 21 formed of a plurality of grooves formed in the longitudinal direction at predetermined intervals, and inclined downward in the transfer direction;

The injection unit 30 is located in the upper portion of the rear end of the conveying unit, has a plurality of needles, and descends to the conveying unit to form a wound or a gap in the larvae transferred to the conveying unit groove;

an air injection unit 40 located at the upper portion of the conveying unit and a front end of the injection unit, and spraying air in the direction of the conveying unit groove or the injection unit needle;

an immune inducing unit 50 having an immune inducing solution, located below the rear end of the transfer unit, in which the accommodated larvae are immersed in the immune inducing solution, and the immune inducing solution is injected into the larvae through a wound or crevice; and

It is located at the lower end of the transfer unit, and relates to an automated immunogen injection system for mass production including a vibrating unit 60 for vibrating the transfer unit.

The immunogen injection automation system of the present invention removes the injection part pressure plate and adjusts the needle to be inserted only to a part of the groove of the injection line 21 (control so that the needle does not penetrate the larvae) It is possible to minimize the death of the larvae from wounds and shock. .

In the immunogen injection automation system of the present invention, the transfer unit in which the injection line is formed is located inclined, and the larvae are moved along the injection line of the transfer unit by the transfer unit, and the injection by the injection unit and the movement by the air injection unit are continuously performed. Compared to the method of transferring the loading part containing derivate or larvae to the transfer part, mass production is possible and work efficiency can be improved.

The immunogen injection automation system of the present invention can increase mobility and work space efficiency by providing a vibration damping unit to reduce vibration between the transfer unit and the support unit and a wheel at the lower end of the support unit.

The immunogen injection automation system of the present invention immerses the larvae into the immune induction part after stabbing the larvae with a needle (unlike the method of immersing the larvae in a state where the larvae are stabbed with a needle), so the larvae can be soaked in the immune inducer for a longer time. Therefore, it is possible to increase the injection amount of the immune inducer to the maximum.

1 is a perspective view of an automated immunogen injection system of the present invention.
2 is a side view of the automated immunogen injection system of the present invention.
3 is a view showing the transfer unit and the injection unit of FIG. 1 .
FIG. 4 is a more enlarged view of FIG. 3 .
FIG. 5 illustrates the vibrating unit in FIG. 1 .
6 shows the operation of the injection unit.
7 shows the injection unit of Korean Patent Registration No. 10-1896842.

Hereinafter, a preferred embodiment of the present invention will be described with reference to the drawings. However, the scope of the present invention is not limited to the description or drawings of the following embodiments. That is, the terms used herein are used only to describe specific embodiments, and are not intended to limit the present invention. The singular expression includes the plural expression unless the context clearly dictates otherwise. In addition, terms such as "comprises" or "have" described in this specification are intended to designate that the features, numbers, steps, operations, components, parts, or combinations thereof described in the specification exist, but one or the It should be understood that the existence or addition of the above other features or numbers, steps, operations, components, parts or combinations thereof is not precluded in advance.

In addition, terms such as "unit", "group", and "module" described in the specification mean a unit that processes at least one function or operation, which may be implemented as hardware or software or a combination of hardware and software.

1 is a perspective view of the automated immunogen injection system of the present invention, FIG. 2 is a side view of the automated immunogen injection system of the present invention, FIG. 3 shows the transfer unit and the injection unit of FIG. 1 , and FIG. 4 is a view of FIG. 3 more It is an enlarged view, and FIG. 5 shows the vibrating unit in FIG. 1 , and FIG. 6 shows the operation of the scanning unit.

1 to 6 , the automated larval immunogen injection system of the present invention includes an input unit 10 , a transfer unit 20 , an injection unit 30 , an air injection unit 40 , an immune induction unit 50 and a vibrating unit. (60).

Larvae (a) may be insect larvae (mealworms, larvae, silkworms, slugs, etc.), and there is no particular limitation thereto. For example, it could be a silkworm larva that can be utilized as a feed additive.

The input unit 10 may separate and discharge the agglomerated larvae. The input unit 10 may use a known vibrating screen. For example, the input unit 10 may include a housing, a vibration motor positioned inside the housing, and a screen (slanted at a predetermined angle).

The transfer unit 20 is located at the rear end of the input unit, and the scanning lines 21 formed of a plurality of grooves formed in the longitudinal direction are formed at predetermined intervals, and are inclined downward in the caterpillar transfer direction.

The transfer unit 20 is formed with a plurality of grooves 21 in the longitudinal direction.

For example, the transfer unit may include a plate 22 in which a plurality of grooves 21 are formed and a guard frame 23 forming a side surface of the plate.

For example, the plate having a plurality of grooves may be made of acrylic, stainless steel, aluminum, or the like.

The groove may have a width and depth sufficient to insert a caterpillar. For example, the groove may have a width of 3 to 20 mm and a depth of 3 to 20 mm.

The groove may be a passage through which the larvae are transported.

In addition, the needles of the injection unit may be positioned directly above the groove, respectively, and the needle of the injection unit may be an injection line that descends and stabs the larvae.

The transfer unit 20 is inclined downward in the caterpillar transfer direction, for example, the inclination angle may be 1 ~ 45 °.

A lubricating film may be coated on the upper surface (the upper surface between the grooves) and the groove of the transfer unit. The lubricating film lowers the larvae's ability to attach adhesively, so that the larvae can be more easily put into the grooves. A known lubricant may be used for the lubricating film. For example, the lubricant includes silicone wax, polyolefin wax, Teflon coating agent, molybdenum coating agent, and the like.

The vibrating unit 60 is located at the lower end of the conveying unit and may vibrate the conveying unit.

Referring to FIG. 5, the vibrating unit 60 is coupled to both ends of the motor 61, the first shaft 62, the second shaft 63, the first shaft and the second shaft, and has a rotating weight therein (Fig. (not shown) is located on the cover 64, the first belt 65 for shearing the rotational force from the motor to the first shaft, the second belt 66 for transmitting the rotational force from the first shaft to the second shaft, and the rotary weight Transmitting the generated vibration to the transfer unit may include a connection unit 67 .

The connection part 67 may transfer the vibration generated by bonding to the transport part to the transport part.

That is, the vibrating unit generates vibration by transmitting the rotational force of the motor to the rotating weight, and the generated vibration is transmitted to the conveying unit.

Since the immunogen injection automation system of the present invention can move the larvae (in the forward direction) to the groove of the transfer unit by vibrating the inclined transfer unit 20, the conventional process of moving forward and backward the loading box containing the larvae or the immersion solution is stored Compared to that, since the transfer method is continuous, work efficiency can be improved.

The injection unit 30 is located above the rear end of the transfer unit, has a plurality of needles, and descends to the transfer unit to form a wound or a gap in the caterpillar transferred to the transfer unit groove.

The injection unit 30 includes a plate-shaped fixing plate 31 to which the tip of the needle is fixed, a plurality of needles 32 having a needle tip positioned directly above the scanning line 21, and the fixing plate up and down. It includes a driving unit 33 for moving.

When the fixed plate descends to the transfer unit, the needles are arranged on the fixed plate 41 so that the needle 32 is positioned in the groove 21 of the transfer unit. That is, the needles may be arranged at predetermined intervals on the fixing plate to correspond to the grooves of the loading part.

For example, the driving unit 33 may include a motor 331 and a connector 332 to convert the rotational motion of the motor into a linear motion to move the fixed plate up and down.

The injection unit may be installed to be inclined downward at the same angle as the transfer unit.

The injection part fixes the support bar 34 to the lower frame 1 and the inclined frame 2 by giving an inclination at the same angle as the transfer part, and the upper plate 35 coupled to the support bar, the inner surface of the fixing plate 31 It may include a connection plate 36 coupled to the side surface of the connector 332 and a spring bar 37 providing elasticity to the connection plate 36 .

When the injection unit descends and stabs the larva, the needle may be inserted within 3/4 of the bottom depth (h) of the groove 21 of the conveying unit, preferably in the range of 1/100 to 3/4. When the needle is inserted thinly within 1/100 of the bottom depth (h), the induction of the immune-inducing fluid through wounds or crevices in the larvae is not smooth, and the needle is inserted deep enough to exceed 3/4 of the bottom depth (h). In this case, the mortality rate of the larvae increases.

The injection part is a needle syringe interval of 5 to 50 mm, and the needle thickness is 0.2 mm to 0.5 mm, preferably 0.35 ± 0.5 mm. At one time of induction of immunity, up to 4 needles per larva can be inserted into the larva at the same time.

The air injection unit 40 may be positioned above the conveying unit and at the front end of the injection unit, and may inject air in the direction of the conveying unit groove or the injection unit needle.

The air injection unit 40 is provided with an air supply unit 41, a distribution unit 42, and a plurality of injection bars 43, it is possible to forcibly transport the larvae by injecting air after injection by the injection unit. .

The distribution unit 42 may be a pipe positioned above the transfer unit. The distribution part 42 may be fixed in the longitudinal direction of the transfer part.

The injection bar 43 may be a micro tube having a predetermined length. One end of the injection bar 43 is coupled to the distribution unit to receive air, and the other end is located directly above the groove 21 , but the air discharge direction of the other end may be the needle direction of the injection unit.

The injection bar 43 can forcibly discharge not only the larvae staying in the grooves but also the body fluid that flows out during injection by injecting air into the larvae after the immune induction injection along the groove 21 or the injection line, so that the larvae transfer efficiency can increase

The immunogen injection automation system of the present invention may further include a vibration damping unit 80 to reduce vibration between the transfer unit and the support unit 70 .

Due to the vibration damping part, the system of the present invention can include the wheel 3 at the lower end by greatly reducing the intensity of vibration transmitted to the support part.

The immunity inducing unit 50 may be a storage tank in which a predetermined amount of the immune inducing liquid is loaded. The immune induction unit 50 is located below the rear end of the transfer unit.

The immune-inducing fluid may be a substance having an effect of regulating immune ability without being toxic to the living body. For example, the immune inducing fluid may be a biological response modifier (BRM), specifically, AHCC (Active Hexose Correlated Compound), Polysaccarides, Polysaccaride peptides, Nucleosides, Triterpenoids, etc. A variety of substances can be applied to the present invention as an immune inducer.

The larvae may be immersed in the immune induction part for 1 second or more.

When the larvae are immersed in the immune inducing part, the immune inducing solution may be injected into the larvae through a wound or crevice formed by the needle.

The immunogen injection automation system of the present invention can immerse the larvae in the immune inducer for a longer period of time, thereby maximally increasing the injection amount of the immune inducing liquid.

The immunogen injection automation system may include a sensor for detecting the operation of the vibration unit, the injection unit, the transfer unit, and the air injection unit, or a control unit for controlling them.

The control unit can control whether each device is operated, operating time, number of vibrations and feed rate, whether the injection unit rises and falls, the depth of descent of the injection unit (the insertion depth of the needle groove), and the descent and rise time of the injection unit.

The control unit can execute the operation of the immunogen injection automation system by having a user's input condition or operation process (algorithm) built-in and systemized in the form of a program command.

In another aspect, the present invention relates to a method for automated injection of an immunogen for mass production. The immunogen injection automation method may use the automated immunogen injection system of FIGS. 1 to 4, but is not limited thereto.

The immunogen injection automation method includes an input step, a transfer step, an injection step, an air spray step, and an immune induction step.

The input step is a step to separate and discharge the larvae that are agglomerated. For example, in the input step, using a known vibrating screen, it is possible to separate and discharge the clumped larvae.

The transfer step is a step of moving the larvae to the lower part of the injection unit 30 using the transfer unit 20 and the vibrating unit 60 .

In the transfer step, the larvae can be moved (in the forward direction) to the groove 21 of the transfer unit by vibrating the transfer unit 20 with the vibrating unit. Compared to that, since the transfer method is continuous, work efficiency can be improved.

The transfer unit 20 and the vibrating unit 60 may refer to the above-described contents.

Referring to FIG. 4 , the injection step is a step in which the injection unit 30 descends to the conveying unit, stabs the caterpillar located in the groove of the conveying unit with a needle, and ascends again.

The scanning step may be repeated according to a set time, and when the scanning step is entered, the motor operation of the vibrator is stopped. For example, the present invention enters the scanning phase at intervals of 30 seconds, and the scanning phase may be set to 2 seconds or 3 seconds. That is, in the present invention, when the vibrating unit is operated for 30 seconds and the caterpillar advances, the operation of the vibrating unit is stopped (after 30 seconds), and the injection unit descends to stab the caterpillar and then rises (2 to 3 seconds), the vibration The sub can be activated again.

As described above, when the injection unit descends and stings the caterpillar, the control unit removes the needle of the injection unit within 3/4 of the bottom depth (h) of the groove 21 of the conveying unit, preferably 1/100 ~ You can control it to insert in a 3/4 range.

The injection unit 30 may refer to the above-mentioned contents.

The air jetting step is a step of jetting air along the groove (scan line, 21) of the conveying unit using the air jetting unit 40 .

The air injection step is started when the injection unit rises after descending, is stopped after operating for a predetermined time (eg, 1 second to 20 seconds after the air injection unit is operated), and is operated when the injection unit descends and then rises again.

In the air injection step, by injecting air along the groove 21 or the injection line, not only the caterpillars staying in the conveying unit groove 21 but also the body fluid flowing out during injection can be forcibly discharged, thereby increasing the feeding efficiency of the caterpillars. .

The control unit may control the operation of the vibrating unit, the air injection unit, etc. by determining whether the scanning unit is rising or falling through a detection sensor (eg, an infrared sensor, a contact sensor, etc.).

In the immune induction step, when the larvae are immersed in the immune inducing part, the immune inducing fluid is injected into the larvae through a wound or crevice formed by a needle.

The immune induction step may be 1 second or more.

The air injection unit and the immune induction unit may refer to the above-described contents.

Hereinafter, the present invention will be described in more detail through examples, but the present invention is not limited to these examples.

Example 1

The survival rate after silkworm injection was measured using the injection part of FIGS. 1 to 6 .

Comparative Example 1

The survival rate after silkworm injection was measured using the injection unit (refer to FIG. 7) disclosed in Korean Patent Registration No. 10-1896842 (the applicant's patent).

The survival rates of Example 1 and Comparative Example 1 are shown in Table 1 below.

elapsed time Comparative Example 1 Example 1 1 hour later 93.1±4.4% 98.51±2.91% after 20 hours 72.31±1.5% 97.9±0.86%

Referring to Table 1, compared to Comparative Example 1 in Example 1, the survival rate at 1 hour after injection was improved by 2.22%, and the survival rate after 20 hours was improved by 22.59%.

Those of ordinary skill in the art to which the present invention pertains will understand that it can be implemented in a modified form without departing from the essential characteristics of the present invention. Therefore, the disclosed embodiments are to be considered in an illustrative rather than a restrictive sense. Therefore, the scope of the present invention is not limited to the above-described embodiments, and should be construed to include various embodiments within the scope of the claims and equivalents thereto.

Claims (7)

The input unit 10 for separating and discharging the agglomerated larvae;
The transfer unit 20 is located at the rear end of the input unit, the scan lines 21 formed of a plurality of grooves formed in the longitudinal direction are formed at predetermined intervals, and inclined downward in the transfer direction;
The injection unit 30 is located on the upper rear end of the conveying unit, has a plurality of needles, and descends to the conveying unit to form a wound or a gap in the larvae transferred from the conveying unit groove;
an air injection unit 40 located above the transfer unit and a front end of the injection unit, and injecting air into the scanning line 21;
Immune inducing unit 50 having an immune inducing solution, located below the rear end of the transfer unit, in which the accommodated larvae are immersed in the immune inducing solution, and the immune inducing solution is injected into the larvae through a wound or crevice; and
It is located at the lower end of the transfer unit and includes a vibrating unit 60 that vibrates the transfer unit,
The air injection unit 40 is an automated immunogen injection system having a plurality of injection bars 43,
The vibrating unit 60 vibrates the conveying unit to insert the caterpillar supplied from the input unit into the scanning line 21 of the conveying unit, and moves the caterpillar to the lower part of the injection unit,
When the injection unit 30 operates, the operation of the vibrating unit is stopped, and the needle of the injection unit descends and rises after stabbing the caterpillar within 3/4 of the bottom depth (h) of the transfer unit scanning line 21,
When the needle of the injection unit rises, the vibrating unit and the air injection unit are operated, and the injection bar 43 of the air injection unit injects air in the direction of the immune induction unit along the injection line 21 to transfer the larvae to the immune induction unit. Immunogen injection automation system for mass production, characterized in that [Claim 2] The automated immunogen injection system for mass production according to claim 1, wherein the automated immunogen injection system further comprises a vibration damping unit (80) for reducing vibration between the transfer unit and the support unit (70). delete According to claim 1, wherein the vibrating unit 60 is coupled to both ends of the motor 61, the first shaft 62, the second shaft 63, the first shaft and the second shaft, therein a rotating weight ( (not shown in the drawing) is located, the first belt 65 for shearing the rotational force from the motor to the first shaft, the second belt 66 for transmitting the rotational force from the first shaft to the second shaft, and the rotating weight Immunogen injection automation system for mass production, characterized in that the transfer unit for transmitting the vibration generated in the transfer unit includes a connection unit (67). delete delete [Claim 2] The automated system for mass production immunogen injection according to claim 1, wherein the injection unit has a needle injection interval of 5 to 50 mm and a needle thickness of 0.2 mm to 0.5 mm.

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