KR102345627B1 - Laser crystallization apparatus capable of selective crystallization of optical isomers, crystallization and observation method using the same - Google Patents

Abstract

One aspect of the present invention discloses a crystallization apparatus using a laser. Another aspect of the present invention discloses an apparatus for selective crystallization of optical isomers using a laser. Another aspect of the present invention provides a method for selective crystallization and observation of a crystalline material using the above-described crystallization apparatus. Another aspect of the present invention provides a method for selectively crystallizing and observing l-form or d-form by controlling the molecular asymmetry (chirality) of an optical isomer crystal material through a handedness control of circularly polarized laser light. The crystallization apparatus of the present invention includes: a stage (130); a light collecting unit (120); an observation unit (110); and a light source unit (140).

Description

Crystallization apparatus using laser capable of selective crystallization of optical isomers, crystallization and observation method using the same

The present invention relates to a crystallization apparatus using a laser, and a crystallization and observation method using the same.

Crystallization is to form solid particles having a certain shape and size from a uniform liquid phase. Most pharmaceuticals and organic fine chemicals are sold in crystallized form. The reason that crystallization plays such an important role as the final stage of refining is, above all, the purity of the crystal. In addition, since crystallization produces crystals of a certain size, it plays an important role in performing finishing steps such as subsequent filtration or drying.

Recrystallization, which can be said to be an applied technology of crystallization, is a purification technique that separates impurities that are undesirably dissolved in the mother liquor and high value-added crystal products. Strictly speaking, recrystallization is the process of redissolving and recrystallizing an initially solidified crystallized material again to produce an end product crystal of the desired size, shape, purity and yield. Conventionally, since this recrystallization process was performed by dissolving a large amount of solute in a large amount of solvent, it was made in batch units. Recrystallization of such a batch unit has a disadvantage in that it is difficult to control the crystallization conditions, particularly temperature, and to observe them immediately. Therefore, there is a need for a recrystallization method that allows precise control of the temperature conditions of the recrystallization process and at the same time enables detailed observation with the naked eye, which facilitates quantitative identification of the crystallization rate.

Optical tweezers focus the laser on a specific spot to generate a difference in momentum caused by the laser to particles in the path of laser light. technology to capture Existing experiments have used optical tweezers for the purpose of capturing specific particles or measuring the interaction between a number of captured particles.

An enantiomer refers to a case in which two molecules with optical activity form a mirror-symmetric relationship. It is generally used synonymously with enantiomer. Molecules exhibiting optical isomerism do not overlap with mirror-image molecules through rotation, but have carbon or chemical bonds of carbon, and stereocenters such as nitrogen and sulfur. It also has a plane of symmetry that bisects itself within a molecule. In order to distinguish optical isomers from each other in the nomenclature, when 4 or more substituents are different, they are classified into R/S designations (Rectus, Sinister) according to the Khan-Ingold-Prelog ranking rule (CIP rule), and the rotational polarization phenomenon It is measured within a certain condition, and it is sometimes classified by d/l cadastral (Dextrorotation, Levorotation). For d/l cadastrals, from the observer's point of view, dextrorotation means clockwise or right-handed rotation, and levorotation means counter-clockwise or left-handed rotation.

In the food and agrochemical industries, particularly the pharmaceutical industry, it is important to produce pure enantiomers. Today, chiral drugs often show that only one enantiomer provides the desired physiological effect, and in many cases the other enantiomer is ineffective or even detrimental. Accordingly, regulatory agencies are demanding that chiral pharmaceuticals be administered in optically pure form. This has facilitated efforts in industry and academia to develop technologies that can produce pure enantiomers, but a technique for separating the d-form and l-form of optical isomers using a polarized laser has not been reported yet. .

The present invention is to solve the problems of the prior art described above, and an object of the present invention is to enable partial dissolution and recrystallization of a crystalline material using optical tweezers, and locally irradiate a laser to the crystalline material It is possible to provide a crystallization apparatus using a laser, which enables selective crystallization and recrystallization of optical isomers using a polarized laser, and enables observation of the crystallization phenomenon in real time with an optical microscope, and a crystallization and observation method using the same.

One aspect of the present invention is a stage 130 for supporting, transporting and temperature control of the specimen; a light collecting unit 120 forming an enlarged image of a portion of the specimen; an observation unit 110 for observing the image; and a light source unit 140 for generating laser light irradiated in the direction of the stage 130 through the light collecting unit 120 .

In one embodiment, the stage 130 may include a heating means in contact with the upper and lower portions of the specimen.

In one embodiment, the crystallization apparatus 100 may include an adjustment device for adjusting the mutual spacing of two or more of the stage 130 , the light collecting unit 120 , and the observation unit 110 .

In an embodiment, the light collecting unit 120 may include two or more light collecting means having different focal lengths.

In an embodiment, the type of laser light generated by the light source unit 140 may be one selected from the group consisting of a solid laser, a gas laser, a semiconductor laser, a dye laser, a pulse laser, a fiber laser, and a chemical laser. .

Another aspect of the present invention provides a method of using the crystallization apparatus 100, comprising: (a) preparing a sample containing a saturated solution of the sample; (b) placing the sample on a stage 130 and adjusting the temperature of the stage 130 to form crystals; and (c) partially dissolving and recrystallizing the crystals by irradiating laser light.

In one embodiment, the sample may be a crystalline material.

In one embodiment, in step (c), the process of partially dissolving and recrystallizing by irradiating the laser light to the crystal formed in step (b) may be observed in real time through the observation unit 110 .

In one embodiment, in the step (c), the crystal or its droplet is captured with optical tweezers using the laser light, and it is intended in a direction perpendicular to the traveling direction of the laser light. direction can be moved.

Another aspect of the present invention provides an apparatus 100' for selective crystallization of optical isomers using a laser, further comprising a polarization unit 150' located in the path of the laser light in the crystallization apparatus 100 do.

In an embodiment, the polarizer 150 ′ may include a linear polarizer and a quarter-waveplate.

In addition, another aspect of the present invention, in the method using the selective crystallization apparatus 100', (i) preparing a sample containing a saturated solution of the sample; (ii) placing the specimen on a stage 130' and adjusting the temperature of the stage 130' to form crystals; and (iii) partially, selectively dissolving and recrystallizing a crystal of a specific phase among optical isomers by irradiating the sample with polarized laser light; do.

In one embodiment, the steps (ii) and (iii) may be performed simultaneously or sequentially.

In one embodiment, the sample may be a crystalline material in which optical isomers exist.

In an embodiment, in the step (iii), the polarized laser light is circularly polarized laser light passing through a linear polarizing plate and a phase retarder, and the handedness of the circularly polarized laser light can be controlled. have.

In one embodiment, in the step (iii), the observation unit 110' irradiates the polarized laser light to the crystal formed in the step (ii) to partially dissolve and recrystallize the crystal of a specific phase among optical isomers. ) can be observed in real time.

In one embodiment, in the step (iii), the crystal or its droplet is captured with optical tweezers using the laser light, and it is intended in a direction perpendicular to the traveling direction of the laser light. direction can be moved.

In one embodiment, after step (iii), (iv) observing the product of step (iii) using a polarizing microscope; may further include.

According to one aspect of the present invention, the crystalline material may be partially dissolved and recrystallized by locally irradiating or blocking the laser using optical tweezers.

According to another aspect of the present invention, selective crystallization and recrystallization of d-form and l-form of a crystalline material are possible through handedness control of circularly polarized laser light.

In addition, according to another aspect of the present invention, it is possible to observe the crystallization and recrystallization process in real time.

It should be understood that the effects of the present invention are not limited to the above-described effects, and include all effects that can be inferred from the configuration of the invention described in the detailed description or claims of the present invention.

1 is a block diagram of a crystallization apparatus and an apparatus for selective crystallization of optical isomers according to an embodiment of the present invention.
2 is a schematic diagram illustrating a method of converting linearly polarized light into circularly polarized light using a phase delay.
3 is an image illustrating the difference between right-handed circularly polarized light and left-handed circularly polarized light.
4 is an optical microscope image of crystal dissolution-recrystallization according to laser irradiation or blocking according to an embodiment of the present invention.
Figure 5 shows the structure of the crystals (d-form and l-form) in pure morphology.
6 shows an optical microscope image of a process of repeatedly dissolving and recrystallizing the crystal according to an embodiment of the present invention.
7 shows a polarization microscope image when the crystal and recrystallization are observed after rotating a polarizer 6 to 7° in a counter-clockwise direction in a module of a polarization microscope.

Hereinafter, the present invention will be described with reference to the accompanying drawings. However, the present invention may be embodied in several different forms, and thus is not limited to the embodiments described herein. And in order to clearly explain the present invention in the drawings, parts irrelevant to the description are omitted, and similar reference numerals are attached to similar parts throughout the specification.

Throughout the specification, when a part is "connected" with another part, this includes not only the case where it is "directly connected" but also the case where it is "indirectly connected" with another member interposed therebetween. . In addition, when a part "includes" a certain component, this means that other components may be further provided without excluding other components unless otherwise stated.

When a range of numerical values is recited herein, the values have the precision of the significant figures provided in accordance with the standard rules in chemistry for significant figures, unless the specific range is otherwise stated. For example, 10 includes the range of 5.0 to 14.9 and the number 10.0 includes the range of 9.50 to 10.49.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

Crystallization device using laser

1 is a block diagram of a crystallization apparatus 100 using a laser according to an embodiment of the present invention. Referring to Figure 1, an aspect of the present invention is a stage 130 for supporting, transporting and temperature control of a sample; a light collecting unit 120 forming an enlarged image of a portion of the specimen; an observation unit 110 for observing the image; and a light source unit 140 for generating laser light irradiated in the direction of the stage 130 through the light collecting unit 120 .

The stage 130 may include heating means in contact with the upper and lower portions of the specimen. When the heating means is in contact with only one side of the upper or lower portion of the sample, the upper and lower portions of the sample are not heated uniformly, resulting in poor temperature uniformity, and since heat is applied only to one side of the sample, temperature control may not be easy either. have. On the other hand, if the heating means is configured in a form in contact with both the upper and lower portions of the sample, excellent temperature uniformity of the sample can be ensured, and the degree of crystallization can be easily controlled by having precise temperature control ability. .

The observation unit 110 may be configured as an eyepiece, a camera, or both. The camera may be one selected from the group consisting of a film camera, a CMOS camera, and a CCD camera, and preferably a CCD camera, but is not limited thereto.

The crystallization apparatus 100 may include an adjusting device for adjusting the mutual spacing of two or more of the stage 130 , the light collecting unit 120 , and the observation unit 110 . By adjusting the mutual spacing of two or more of the stage 130, the light collecting unit 120, and the observation unit 110 using the adjusting device, an enlarged real image of the part of the specimen generated by the light collecting means is obtained. It is focused at a suitable place inside the focal length of the observation unit 110, and the observation unit 110 can make a virtual image well visible at a clear distance based on the real image and observe it clearly. In addition, by highly focusing the laser light on the specimen in the same way, partial dissolution and recrystallization of the crystal are possible.

The light collecting unit 120 may include two or more light collecting means having different focal lengths. By selecting an appropriate light collecting means from among two or more light collecting means having different focal lengths, the magnification of the image observed through the observation unit 110 can be adjusted by adjusting the sizes of the real image and the virtual image, and the focus of the laser light can be adjusted. By focusing the laser highly on the specimen, the crystals can be partially dissolved and recrystallized using optical tweezers.

The type of laser light generated by the light source unit 140 may be one selected from the group consisting of a solid laser, a gas laser, a semiconductor laser, a dye laser, a pulse laser, a fiber laser, and a chemical laser, but is not limited thereto. .

Selective crystallization of optical isomers using laser

1 is a block diagram of an apparatus 100' for selective crystallization of optical isomers using a laser according to an embodiment of the present invention. Referring to FIG. 1 , in another aspect of the present invention, in the crystallization apparatus 100 , the apparatus for selective crystallization of optical isomers using a laser further comprising a polarization unit 150 ′ positioned in a path of the laser light. (100') is provided. The polarizer 150 ′ may be installed at any position shown in FIG. 1 as long as it is on the traveling path of the laser light.

The polarizer 150' may include a linear polarizer and a quarter-waveplate. By passing the linearly polarized laser light passing through the linear polarizer through the phase retarder, it is possible to convert the laser light into circularly polarized laser light. By controlling the handedness of the circularly polarized laser light, it is possible to induce selective crystallization and recrystallization of the d-form and l-form of the optical isomer crystal material.

Recrystallization and observation method using a crystallizer

Another aspect of the present invention provides a method of using the crystallization apparatus 100, comprising: (a) preparing a sample containing a saturated solution of the sample; (b) placing the sample on a stage 130 and adjusting the temperature of the stage 130 to form crystals; and (c) partially dissolving and recrystallizing the crystals by irradiating laser light.

The sample may be a crystalline material. A crystalline material is an arrangement in which specific structural units such as atoms, atomic groups, molecules, and ions are repeated according to a certain rule, and these structural units are referred to as lattice structures or crystal structures. Specifically, the crystalline material may be one selected from the group consisting of an ionic crystal, an atomic crystal, a molecular crystal, a metal crystal, and a crystalline polymer, for example, the ionic crystal material is sodium chloride (NaCl), sodium bromide (NaBr), sodium iodide (NaI), sodium oxide (Na ₂ O), sodium chlorate (NaClO ₃ ) sodium sulfide (Na ₂ S), sodium sulfate (Na ₂ SO ₄ ), potassium iodide (KI), magnesium sulfide (MgS), magnesium iodide (MgI ₂ ), lithium chloride (LiCl), calcium chloride (CaCl ₂ ), zinc chloride (ZnCl ₂ ), aluminum oxide (Al ₂ O ₃ ), calcium oxide (CaO), magnesium oxide ( MgO), the molecular crystal material may be an organic compound such as hydrocarbon, an inert gas, dry ice, iodine, ice, etc., and the metal crystal material may be a metal such as gold, silver, copper, iron, or aluminum. However, the present invention is not limited thereto. The crystalline polymer is a concept arranged with an amorphous polymer, and at least some of the internal chains are regularly arranged, for example, a lamella formed by a folded chain by an attractive force between the chains. ) structure may be included. Specifically, it refers to a polymer whose crystal structure can be confirmed by X-ray diffraction, for example, polyethyl, polyacrylonitrile, polypropylene, polystyrene, cellulose, protein, etc., but is not limited thereto.

In one non-limiting example of the present invention, step (a) is, (a-1) preparing a saturated solution of the sample at a temperature higher than the desired temperature and then lowering it to the desired temperature, preparing a saturated solution; (a-2) placing preparat on a hot plate adjusted to a temperature higher than that of the saturated solution of the sample; (a-3) applying an epoxy adhesive in a hollow square shape on the slide glass of the preparat, dropping the saturated solution of the sample prepared in the step (a-1) thereinto, and covering the slide glass; And (a-4) waiting until all the epoxy adhesive is hardened on the hot plate to prepare a sample; may include. When preparing a saturated solution of a sample, the desired temperature varies depending on the type of the sample, and a temperature condition used for general crystallization may be used.

The step (b) includes: (b-1) setting the temperature of the stage 130 high enough to prevent crystals from being formed in the sample, and then putting the sample prepared in step (a) into the stage 130; and (b-2) gradually lowering the temperature of the stage 130 to form a crystal.

The step (c) includes: (c-1) partially dissolving the crystal by irradiating laser light; (c-2) blocking the laser light to recrystallize the dissolved crystal; and (c-3) repeatedly performing steps (c-1) and (c-2). As described above, by repeatedly controlling the partial dissolution and recrystallization of the crystal using a laser, the purity of the crystal is increased and a crystal close to a pure morphology can be obtained. Its mechanism of action is that when recrystallization is caused after dissolving the crystal by using the laser, recrystallization starts from a portion having large defects in the crystal form due to impurities, the impurities are removed and the crystal close to a purer form this will be created

In addition, by repeatedly controlling the partial dissolution and recrystallization of the crystal as described above, it is also possible to measure the crystallization rate of various crystalline materials, which has been difficult to perform conventionally because the recrystallization process is performed in batches.

In step (c), the process of partially dissolving and recrystallizing by irradiating the laser light to the crystal formed in step (b) can be observed in real time through the observation unit 110 . As the actual observation becomes possible, detailed observation with the naked eye is possible, and thus the crystallization rate of various crystalline materials can be quantitatively identified.

In step (c), the crystal or its droplet is captured with optical tweezers using the laser light, and it can be moved in a direction perpendicular to the direction of the laser light. . As such, optical tweezers can be used to capture specific particles or to measure interactions between a large number of captured particles. Furthermore, in the case of the laser light used in step (c), the function of focusing the laser on a specific spot of the crystal is the same as that of the existing optical tweezers, and around the focal point of the laser among the crystals. Inducing a local temperature rise of the portion enables partial dissolution and recrystallization of the crystal.

Crystallization, recrystallization and observation method using selective crystallization apparatus of optical isomers

Another aspect of the present invention provides a method using the selective crystallization apparatus 100', comprising: (i) preparing a sample containing a saturated solution of the sample; (ii) placing the specimen on a stage 130' and adjusting the temperature of the stage 130' to form crystals; and (iii) partially, selectively dissolving and recrystallizing a crystal of a specific phase among optical isomers by irradiating the sample with polarized laser light; do.

Steps (ii) and (iii) may be performed simultaneously or sequentially. When the two steps are performed simultaneously, by controlling the temperature of the stage while irradiating the polarized laser light to the specimen, the optical isomer of a specific phase that is not dissolved by the polarized laser light can be selectively crystallized. have. In addition, when the two steps are sequentially performed, the crystals of a specific phase among optical isomers may be partially and selectively dissolved and recrystallized by irradiating the polarized laser light to the crystal formed in step (ii).

The sample may be a crystalline material in which optical isomers exist. Specifically, not only the case where the optical isomer of the crystalline material itself exists, but also the case where the optical isomer in the form of a crystal in which several molecules of the crystalline material are combined into small crystal nuclei may be included.

In step (iii), the process of partially dissolving and recrystallizing a crystal of a specific phase among optical isomers by irradiating the polarized laser light to the crystal formed in step (ii) is observed in real time through the observation unit 110' can do.

In step (iii), the crystal or its droplet is captured with optical tweezers using the polarized laser light, and the crystal or its droplet is captured in a direction perpendicular to the traveling direction of the polarized laser light. can be moved

The apparatus used for the selective crystallization, recrystallization and observation method of the optical isomer, the crystal dissolution method, the recrystallization method, the observation method and the effect thereof, the selective crystallization apparatus 100' further includes the polarizing part 150' “Recrystallization and observation using the crystallization device, except that it uses circularly polarized laser light and selectively dissolves and recrystallizes only a specific phase (d-form or l-form) of a crystalline material in which optical isomers exist. Method” is the same as described above.

In the step (iii), the polarized laser light is circularly polarized laser light passing through a linear polarizer and a phase retarder, and handedness of the circularly polarized laser light may be controlled.

2 is a schematic diagram showing a method of converting linearly polarized light into circularly polarized light using a phase retarder, and FIG. 3 is a right-handed circularly polarized light and left-handed It is an image showing the difference of circularly polarized light).

Referring to FIGS. 2 and 3 , it is possible to convert the laser light linearly polarized through the linear polarizing plate into circularly polarized laser light by passing it through the phase retarder. For example, if the linearly polarized laser light perpendicular to each other and having the same amplitude is propagated to have a phase difference of +π/2 or -π/2, right-circularly polarized light rotates clockwise when observed in the direction in which the light travels. The handedness of the circularly polarized laser light may be controlled to generate (right-handed circularly polarized light) or counterclockwise rotated left-handed circularly polarized light. It is known that in the process of crystallization of a crystalline material in which optical isomers exist, l-form and d-form are produced at a ratio of almost 1:1, and when using left-circle and right-circular polarized lasers, d-from and d-form, respectively Since l-form crystals are mainly generated, selective crystallization of d-form and l-form of the optical isomer crystal material can be induced by controlling the handedness of the circularly polarized laser light.

After step (iii), (iv) observing the product of step (iii) using a polarizing microscope; may further include. By observing the product of step (iii) using a polarizing microscope, it can be confirmed whether a crystal having any phase of d-form and l-form is recrystallized.

Hereinafter, an embodiment of the present invention will be described in more detail. However, the following experimental results describe only representative experimental results among the above examples, and the scope and contents of the present invention may not be construed as being reduced or limited by the examples. Each effect of the various embodiments of the present invention not explicitly presented below will be specifically described in the corresponding section.

Preparation Example: Preparation of Specimens

A saturated solution at 60°C was prepared by gradually lowering the temperature by making a saturated solution at 80°C using a crystalline material in which optical isomers exist. After maintaining for about 10 minutes with a saturated solution at 60 °C, stirring was stopped. Preparat was placed on a hot plate adjusted to 70 °C, and the saturated solution was adjusted to the same temperature as the preparat. After 5 minutes, epoxy glue was applied in a hollow square shape on the slide glass in Preparat, and 20 ml of the saturated solution was dropped thereinto. Thereafter, one surface of the cover glass in contact with the saturated solution was treated with an octadecyl trichlorosilane (OTS) solution to cover the slide glass. Then, on the hot plate, the sample was prepared by waiting until all the epoxy adhesive was hardened.

Example 1: Crystallization and recrystallization using a crystallizer

After setting the temperature of the stage 130 of the crystallization apparatus 100 to 60 °C, the sample according to the preparation example was put in the stage 130, and the rate of crystal growth was observed while the temperature was gradually lowered. Then, the process of re-crystallizing by irradiating a laser to the crystal that has been equilibrated at a specific temperature to melt (dissolve), and block the laser to observe the process.

Example 2: Selective Crystallization Using a Selective Crystallization Device

After setting the temperature of the stage 130 of the selective crystallization apparatus 100 ′ in which the polarization unit 150 ′ is installed in the path of the laser light to 60° C., the sample according to the preparation example is put in the stage 130 and the polarization While the laser was irradiated, the rate of crystal growth was observed while gradually lowering the temperature.

Example 2: Selective recrystallization using selective crystallization apparatus

After setting the temperature of the stage 130 of the selective crystallization apparatus 100 ′ in which the polarizing unit 150 ′ is installed in the path of the laser light to 60° C., the sample according to the preparation example is put in the stage 130, and the temperature The rate of crystal growth was observed while gradually lowering the Then, the process of re-crystallizing by irradiating a polarized laser to the sample that was equilibrated at a specific temperature to melt (dissolve), and block the laser to observe the process.

Experimental Example 1: Observation of crystallization and recrystallization process of crystalline material using a crystallizer

An optical microscope image of the process of dissolving and recrystallizing the crystal according to Examples 1 and 2 is shown in FIG. 4 .

Referring to FIG. 4 , when the laser is irradiated, the crystal is dissolved and the size of the crystal decreases over time, and it can be seen that the crystal grows along the crystal plane after the laser is cut off.

Figure 5 shows the crystal structures (d-form and l-form) in pure morphology.

6 shows an optical microscope image of the process of repeatedly dissolving and recrystallizing the crystal according to Examples 1 and 2 above.

Referring to FIGS. 5 and 6 , as the melting and recrystallization of the crystal by laser irradiation are repeated, the size of the crystal is decreased, but it can be confirmed that the crystal is changed to a more pure morphology (prue morphology).

Experimental Example 2: After crystallization and recrystallization using a selective crystallization device, observation using a polarizing microscope

7 shows a polarization microscope image when the crystal and recrystallization are observed after rotating a polarizer 6 to 7° in a counter-clockwise direction in a module of a polarization microscope.

Observe the polarization microscope images of the d-form and l-form of the crystals and recrystallizations prepared according to Examples 2 and 3 above.

Referring to FIG. 7 , among the optical isomers of the crystal and recrystallization, d-form will have white color and l-form will have yellow brown color, so that the crystallization is performed using the polarized laser. It can be confirmed that it is possible to partially and selectively crystallize, dissolve and recrystallize the optical isomers of a specific phase present in

The above description of the present invention is for illustration, and those of ordinary skill in the art to which the present invention pertains can understand that it can be easily modified into other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not restrictive. For example, each component described as a single type may be implemented in a dispersed form, and likewise components described as distributed may be implemented in a combined form.

The scope of the present invention is indicated by the following claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention.

100: crystallization device
100': optional crystallization device
110, 110': observation unit
120, 120': light collecting part
130, 130': stage
140, 140': light source unit
150': polarizer

Claims (17)

Stage 130 for supporting, transporting, and temperature control of the specimen;
a light collecting unit 120 forming an enlarged image of a portion of the specimen;
an observation unit 110 for observing the image; and
In the method of using the crystallization apparatus 100 using a laser, comprising a light source unit 140 for generating laser light irradiated in the direction of the stage 130 through the light collecting unit 120,
(a) preparing a sample containing a saturated solution of the sample;
(b) placing the sample on a stage 130 and adjusting the temperature of the stage 130 to form crystals; and
(c) partially dissolving and recrystallizing the crystal by irradiating laser light; including, recrystallization and observation method using a laser. According to claim 1,
The stage 130 includes a heating means in contact with the upper and lower portions of the specimen, recrystallization and observation method using a laser. According to claim 1,
Recrystallization and observation method using a laser, wherein the crystallization apparatus 100 includes an adjustment device for adjusting the mutual spacing of two or more of the stage 130 , the light collecting unit 120 , and the observation unit 110 . According to claim 1,
The light collecting unit 120 includes two or more light collecting means having different focal lengths, recrystallization and observation method using a laser. The method of claim 1,
The type of laser light generated by the light source unit 140 is one selected from the group consisting of a solid laser, a gas laser, a semiconductor laser, a dye laser, a pulse laser, a fiber laser, and a chemical laser, recrystallization and observation method using a laser. delete The method of claim 1,
The sample is a crystalline material, recrystallization and observation method using a laser. According to claim 1,
Recrystallization and observation method using a laser, in which the process of partially dissolving and recrystallizing by irradiating the laser light to the crystal formed in step (b) is observed in real time through the observation unit 110 in step (c). Stage 130 for supporting, transporting and temperature control of the specimen;
a light collecting unit 120 forming an enlarged image of a portion of the specimen;
an observation unit 110 for observing the image;
a light source unit 140 passing through the light collecting unit 120 and generating laser light irradiated in the direction of the stage 130; and
In the method of using a selective crystallization apparatus 100' of an optical isomer using a laser, including a polarization part 150' located in the path of the laser light,
(i) preparing a sample containing a saturated solution of the sample;
(ii) placing the specimen on a stage 130' and adjusting the temperature of the stage 130' to form crystals; and
(iii) partially and selectively dissolving and recrystallizing crystals of a specific phase among optical isomers by irradiating the sample with polarized laser light; 10. The method of claim 9,
The polarizer 150' includes a linear polarizer and a quarter-waveplate, and a method for selective crystallization, recrystallization and observation of optical isomers using a laser. delete 10. The method of claim 9,
A method for selective crystallization, recrystallization and observation of optical isomers using a laser, wherein the steps (ii) and (iii) are performed simultaneously or sequentially. 10. The method of claim 9,
The sample is a crystalline material in which optical isomers exist, selective crystallization, recrystallization and observation method of optical isomers using a laser. 10. The method of claim 9,
In step (iii), the polarized laser light is circularly polarized laser light passing through a linear polarizing plate and a phase retarder, and adjusting the handedness of the circularly polarized laser light. Selective crystallization, recrystallization and observation methods. 15. The method of claim 14,
A method for selective crystallization, recrystallization and observation of optical isomers using a laser to induce selective crystallization of d-form or l-form by adjusting the handiness of the circularly polarized laser light to left-circular or right-circular polarization. 10. The method of claim 9,
In step (iii), the process of partially dissolving and recrystallizing a crystal of a specific phase among the optical isomers by irradiating the polarized laser light to the crystal formed in step (ii) is performed in real time through the observation unit 110' Observation, selective crystallization, recrystallization and observation method of optical isomers using a laser. 10. The method of claim 9,
After step (iii),
(iv) observing the product of step (iii) using a polarizing microscope; selective crystallization, recrystallization and observation method of optical isomers using a laser, further comprising a.

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