KR102326439B1 - Antimicrobial, anti-inflammatory, antiviral and immunologically enhancing composition comprising ginseng - Google Patents

Abstract

The present invention relates to a composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing ginseng. The composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing ginseng according to the present invention may include any one selected from the group consisting of ginseng, ginseng extract, and mixtures thereof.

Description

Antibacterial, anti-inflammatory, antiviral and immune function improvement composition containing ginseng

The present invention relates to a composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing ginseng. More particularly, it relates to a composition for enhancing immunity including ginseng, which can enhance immune activity including enhancing antibacterial, anti-inflammatory and antiviral activity while including ginseng.

In general, 'health' refers to a state in which there is no abnormality in the body, that is, there is no disease or injury. If you have high immunity, you are less prone to diseases, and even if you do get sick, your recovery is quick. It is self-evident that life expectancy increases with age while maintaining such a healthy state. Therefore, immunity is directly related to human health and lifespan, and it can be said that it is essential to manage immunity in order to maintain health and extend lifespan.

However, looking at the lifestyles of modern people, stress, lack of exercise, irregular life rhythms, unbalanced diet, smoking, etc. are the factors that will lower immunity. Various pollutions are prevalent in the environment, and there are many factors that can also lower immunity. Therefore, modern people should make efforts to enhance immunity.

Looking more specifically at the concept of immunity, it refers to a mechanism for physiologically recognizing and excluding foreign and endogenous foreign substances in the body of humans and animals, and maintaining homeostasis. Immunity can be broadly classified into two categories: innate immunity, which is possessed from birth, and acquired immunity, which is acquired by adapting to life.

Innate immunity is also called natural immunity. It responds non-specifically to antigens and has no specific memory function. The innate immune system includes skin and mucous tissues that block the invasion of antigens, gastric acid of gastric acid, and complement present in the blood. Cells include macrophages, polymorphonuclear leukocytes, and K cells capable of killing infected cells. In fact, most infections are protected by this innate immunity.

Acquired immunity is also called acquired immunity. It can remember the first antigen it invaded, and when it invades again, it can react specifically to remove the antigen effectively. It plays a role in reinforcing the innate immunity.

Acquired immunity includes T cells and B cells. Cells that differentiate into lymphocytes in the epithelial cells of the thymus by a special internal environment and the humoral factors of the thymus are called T cells, and cells that differentiate into lymphocytes in the bone marrow independent of the thymus are called B cells.

The role of T cells is to stimulate the production of antibodies to B cells and directly attack antigens such as tumor cells. , to produce and secrete IgA and IgE antibodies.

As a way to boost immunity, you can take immunostimulants such as chimodulin and Broncovaxom. Immunostimulants contain calf thymus or purified bacterial cells. The mechanism of action of the immune enhancer containing purified bacterial cells is to train the immune system by administering weakened bacteria into the body. will do However, the effect of the immune enhancer is very insignificant, and there are side effects such as a decrease in appetite.

On the other hand, virus means a toxic substance in Latin, and is a group of infectious pathogenic particles that pass through a bacterial filter paper (0.22㎛). Viruses can be classified into bacteriophages, plant viruses, and animal viruses according to the type of host cell, and can be classified into DNA viruses and RNA viruses according to the types of nucleic acids. Recently, various viral diseases such as swine flu, AI, and foot-and-mouth disease have caused great social problems, and accordingly, concerns about effective countermeasures for viral diseases are arousing great social interest.

Currently, the best way to prevent viral diseases is vaccination, but in the case of diseases caused by viruses, the effectiveness of vaccines due to the generation of many viral serotypes (subtypes) is being raised as an important issue. The development and dissemination of inhibitors for virus prevention that can supplement the problems of these vaccines are important. The discovery and development of drugs can be an important drug development method.

Meanwhile, ginseng (Panax ginseng C. A. Meyer), a traditional medicine, is widely known for its efficacy not only in Korea but also in China and Japan, and has been used as a medicinal material in many countries for thousands of years.

Saponin, which shows the main efficacy of ginseng, is a certain compound called glycoside, and is present in the root, stem, and leaf of ginseng. Saponin is composed of about 30 types of ginsenoside, and ginsenoside is effective in promoting health throughout the human body, including prevention of adult diseases and aging, alcohol detoxification and liver protection, anti-inflammatory, improvement of hematopoietic function, and neuroprotection. There is this.

Accordingly, the present applicant does not accompany side effects by using a substance derived from nature, increases the proliferation rate of macrophages, increases the NO production rate, and effectively enhances immunity by supplying a large amount of polyphenols and flavonoids to the body, especially with a smaller content. The present invention was completed by studying a composition that can enhance the immune enhancing effect.

In addition, due to the unique bitter taste of ginseng, the preference of young consumers and foreigners is lowered, and there is a problem that high added value of ginseng products and entry into overseas markets is an obstacle. Therefore, there is a need to provide a food or health functional food with improved palatability while maintaining the immune enhancing activity of ginseng.

KR 10-2018-0088214 A KR 10-2013-0010987 A

It is an object of the present invention to provide a composition capable of enhancing antibacterial, anti-inflammatory and antiviral activity.

An object of the present invention is to provide a composition for enhancing the immune activity of the human body.

An object of the present invention is to provide a food or health food composition with excellent palatability due to excellent flavor while enhancing antibacterial, anti-inflammatory, antiviral and immune activity.

In order to achieve the above object, the composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing ginseng according to an embodiment of the present invention comprises any one selected from the group consisting of ginseng, ginseng extract, and mixtures thereof. it could be

The composition for improving antibacterial, anti-inflammatory, antiviral and immune function including the ginseng may increase cytokine production of TNF-α and IL-12.

The composition for improving antibacterial, anti-inflammatory, antiviral and immune function including the ginseng may further include the milk thistle extract.

The composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing ginseng is Schisandra extract, beetroot extract, gomchi extract, soothing extract, coltsfoot extract, mulberry extract, equestrian extract, amaranth extract, dermis extract, chrysanthemum extract, mugwort It may further include any one selected from the group consisting of extracts and mixtures thereof.

In the composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing ginseng, the ginseng extract may be extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 10 carbon atoms, and mixtures thereof. have.

The health functional food according to another embodiment of the present invention may include a composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing the ginseng.

Hereinafter, the present invention will be described in more detail.

The composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing ginseng according to an embodiment of the present invention may include any one selected from the group consisting of ginseng, ginseng extract, and mixtures thereof.

Ginseng as used herein includes those made by general processing such as drying and pulverization of ginseng.

As used herein, the term 'extract' has the meaning commonly used as a crude extract in the art as described above, but in a broad sense also includes a fraction obtained by additionally fractionating the extract. That is, the plant extract includes not only those obtained using the above-described extraction solvent, but also those obtained by additionally applying a purification process thereto. For example, a fraction obtained by passing the extract through an ultrafiltration membrane having a constant molecular weight cut-off value, separation by various chromatography (prepared for separation according to size, charge, hydrophobicity or affinity), etc. Fractions obtained through various purification methods are also included in the plant extract of the present invention.

The extract used in the present invention may be prepared in a powder state by an additional process such as distillation under reduced pressure and freeze-drying or spray-drying.

As used herein, the term "immune disease" refers to a disease caused by an excessive reaction of the immune system in the human body, and typically includes allergic diseases. However, since the inflammatory response is ultimately caused by the reaction of the immune system in the body, inflammatory disease and immune disease can be used as terms referring to similar ranges in a broad sense, and are not used as distinctly distinct terms in the present specification. , will be mixed.

As used herein, the term "inflammatory disease" is a disease caused by an inflammatory reaction derived from an external or internal substance, and typically includes neuroinflammatory diseases and arthritis.

When any one selected from the group consisting of ginseng, ginseng extract, and mixtures thereof is included, an effect of enhancing immunity including antiviral and antibacterial effects as well as antioxidant and anti-inflammatory activity can be achieved.

The composition for improving antibacterial, anti-inflammatory, antiviral and immune function including the ginseng may increase cytokine production of TNF-α and IL-12.

Cytokines are protein immunomodulators secreted from immune cells, and they bind to specific receptors in autocrine signaling, paracrine signaling, and endocrine signaling to produce an immune response. get involved in There are various types of cytokines involved in cell proliferation, differentiation, apoptosis or wound healing, and in particular, many are involved in immunity and inflammation.

Among these, the most important immune function is performed by a group of cytokines known as interleukin-1,-6,-12, interferon gamma (IFN-γ), and tumor necrosis factor (TNF).

Interleukin (IL) is secreted from leukocytes and is involved in immune system regulation, and more than 30 types are currently known. Interleukin is a substance that plays an important role in the defense of the human body by regulating the immune response by acting on various stages of the immune response.

Among them, IL-12, a cytokine composed of p35 and p40 proteins, has a very important immunological function that connects innate immunity and acquired immunity, and various effector cells involved in the removal of endogenous antigens, i.e., macrophages ), induces activation of NK cells and T-cells. NK cells and T-cells activated by IL-12 stimulation have properties as major factors mediating the initial innate immune response by stimulating the production of IFN-γ. It is also the most important cellular immune mediator because it induces the immune machinery of T-cells to become predominantly Th-1 propensity.

Interferon (interferon, IFN) functions to prevent proliferation of viruses or control cell proliferation, and also plays an important function in the immune system. There are alpha (α), beta (β), gamma (γ), and omega (ω) types of interferon. Interferon-α is mainly secreted by macrophages and interferon-β is mainly secreted by fibroblasts. Interferon-γ is secreted by Th1 cells and NK cells, and interferon-ω is mainly secreted by trophoblasts or Trophoblasts. In particular, interferon-γ (IFN-γ) is produced in T lymphocytes and macrogranulocytes, and is a substance that induces the killing of preyed microorganisms or antigens. In addition, it has various immunomodulatory actions and activates macrophages.

TNF (tumor necrosis factor), a tumor necrosis factor, is a cell signaling protein related to an inflammatory response, and is a cytokine that induces an acute phase response. Tumor necrosis factor is divided into tumor necrosis factor-α (TNF-α) and tumor necrosis factor-β (TNF-β) called lymphotoxin, but in general, tumor necrosis factor refers to tumor necrosis factor-α. .

Therefore, in the case of the immune function improvement effect of the antibacterial, anti-inflammatory, antiviral and immune function improvement composition of the present invention, TNF-α and IL-12 that activate the immune response by using natural products without human toxicity and side effects increase the production of cytokines.

The composition is to prevent a disease selected from the group consisting of allergies, inflammatory diseases and immune diseases.

Allergy refers to various symptoms that are caused by the immune system's overreaction to substances that are not a problem for most people. This includes pollen disease, food allergy, atopic dermatitis, asthma and other hypersensitivity, and shows symptoms such as red eyes, itchy skin rash, runny nose, dyspnea and edema.

Inflammation is a kind of in vivo response to damage or infection of a specific tissue, and the main mediator is immune cells. . When the damaged or infected area of the tissue is small or temporary, it is resolved by acute inflammation, but when the inflamed area is large or chronically infected, it is not resolved by acute inflammation and progresses to chronic inflammation. Chronic inflammation can progress to chronic inflammatory disease.

Immune disease refers to a disease that becomes a problem when a specific immune response occurs, but is not limited thereto, and preferably may be an autoimmune disease, transplant rejection, graft-versus-host disease, and the autoimmune disease includes Crohn's disease, erythema Atopic dermatitis, rheumatoid arthritis, Hashimoto's thyroiditis, pernicious anemia, Addison's disease, type 1 diabetes, lupus, chronic fatigue syndrome, fibromyalgia, hypothyroidism and hyperthyroidism, scleroderma, Behcet's disease, inflammatory bowel disease, multiple sclerosis, myasthenia gravis, Meniere's syndrome, Guilian-Barre syndrome, Sjogren's syndrome, vitiligo, endometriosis, psoriasis, vitiligo, systemic scleroderma, asthma or ulcerative colitis, and the like.

The composition for improving antibacterial, anti-inflammatory, antiviral and immune function including the ginseng may further include the milk thistle extract.

When the milk thistle extract is included in addition to any one selected from the group consisting of ginseng, ginseng extract, and mixtures thereof, the effect of improving immunity may be further enhanced due to a synergistic effect due to interaction.

Preferably, the milk thistle may be mixed in an amount of 1: 3 to 3: 1 parts by weight with respect to any one selected from the group consisting of ginseng extract and mixtures thereof. According to the mixing range, an effective synergistic effect may appear.

Preferably, the composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing ginseng includes any one selected from the group consisting of ginseng, ginseng extract, and mixtures thereof and a milk thistle extract, and 100 parts by weight of the thistle extract. 1 to 10 parts by weight of a seaweed extract; It may include 1 to 5 parts by weight of butterbur extract and 1 to 5 parts by weight of burdock extract.

According to the above range, the production of cytokines of TNF-α and IL-12 is promoted due to the synergistic effect of the interaction, and the anti-inflammatory, antiviral, and antibacterial activity is improved as well as the effect of improving immunity can be further enhanced. . Accordingly, it is possible to exhibit a better immunity improvement effect with a smaller content.

The composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing ginseng is Schisandra extract, beetroot extract, gomchi extract, soothing extract, coltsfoot extract, mulberry extract, equestrian extract, amaranth extract, dermis extract, chrysanthemum extract, mugwort It may further include any one selected from the group consisting of extracts and mixtures thereof.

Preferably, the composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing ginseng may further include a Schisandra extract, a perennial herb extract, a soothing extract, a dermis extract, a chrysanthemum extract, and a cinnamon extract. According to the above range, not only immunity improvement activity is further enhanced, but also the inherent bitter taste of ginseng is neutralized, thereby providing a composition having excellent overall flavor and excellent palatability.

More preferably, the composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing ginseng is 1 to 5 parts by weight of Schisandra extract, 1 to 5 parts by weight of Pungbongnamul extract, 0.1 to 0.1 to soothing extract based on 100 parts by weight of the milk thistle extract. 1 part by weight, 1 to 5 parts by weight of the dermis extract, 1 to 5 parts by weight of the chrysanthemum extract, and 0.1 to 1 parts by weight of the cinnamon extract may be further included.

According to the above range, it is possible to provide a composition having excellent immunity improving activity and excellent palatability. Accordingly, it is possible to provide a food composition or health functional food composition for enhancing immunity that is easy for consumers to access and has no objection to taking it for a long period of time. In particular, due to the synergistic effect of the interaction, it can exhibit better immunity improvement activity while using a smaller amount, so there is no problem such as cytotoxicity due to long-term use,

In the composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing ginseng, the ginseng extract may be extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 10 carbon atoms, and mixtures thereof. have.

The pulverized product of the extract may be eluted with a polar solvent such as an alcohol having 1 to 10 carbon atoms, such as water, ethanol, methanol, or the like, or a mixed solvent having a mixing ratio of 1:0.1 to 1:10 of alcohol and water, more specifically may use water as an extraction solvent. At this time, the extraction temperature may be 10 ℃ to 100 ℃, preferably the extract extracted at room temperature.

The extraction solvent may be used in an amount of 2 to 50 times, preferably 2 to 20 times, based on the weight of the sample. For extraction, the sample may be left for 1 to 72 hours for leaching in the extraction solvent, and in particular, from 1 to 24 hours.

It may be a result obtained by concentrating the filtered extract under reduced pressure with a vacuum rotary concentrator, but is not limited thereto, and includes an extract, a diluted or concentrated solution of the extract, a dried product obtained by drying the extract, and a crude product or purified product thereof.

As a specific example, by using the food composition, it is possible to prepare a processed food capable of enhancing the antibacterial, anti-inflammatory, antiviral and immune function improvement effects. Such processed foods include, for example, sweets, beverages, alcoholic beverages, fermented foods, canned foods, milk products, processed meat foods, noodles, and the like. Confectionery includes biscuits, pies, cakes, breads, candies, jellies, gums, cereals, and the like. Beverages include drinking water, carbonated beverages, functional ionized beverages, functional ionized beverages, juices (eg, apple, pear, grape, aloe, tangerine, peach, carrot, tomato juice, etc.), sikhye, and the like. Alcoholic beverages include sake, whiskey, shochu, beer, Western liquor, fruit wine, and the like. Fermented foods include soy sauce, soybean paste, red pepper paste, and the like. Canned food includes canned aquatic products (e.g., canned tuna, mackerel, saury, conch, etc.), canned livestock products (canned beef, pork, chicken, turkey, etc.), and canned agricultural products (canned corn, peach, file apple, etc.). Processed milk products include cheese, butter, yogurt, and the like. Processed meat products include pork cutlet, beef cutlet, chicken cutlet, and sausage. Includes sweet and sour pork, nuggets, and breadcrumbs. Noodles such as sealed packaged raw noodles are included. In addition to this, the composition may be used in retort foods, soups, and the like.

The food composition according to another embodiment of the present invention is prepared by including the antibacterial, anti-inflammatory, antiviral and immune function improvement composition as an active ingredient.

The health functional food according to another embodiment of the present invention may include a composition for improving antibacterial, anti-inflammatory, antiviral and immune function containing the ginseng.

The health functional food is not particularly limited as long as it can be ingested to improve antibacterial, anti-inflammatory, antiviral and immune activity.

When the health functional food composition of the present invention is used as a food additive, the health functional food composition may be added as it is or used together with other foods or food ingredients, and may be appropriately used according to a conventional method. The active ingredient may be used appropriately depending on the purpose of its use (prevention or improvement). In general, in the production of food or beverage, the health functional food composition of the present invention is added in an amount of 15 parts by weight or less, preferably 10 parts by weight or less, based on the raw material. However, in the case of long-term intake for health purposes, the amount may be less than the above range, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

There is no particular limitation on the type of the health functional food. The health functional food composition of the present invention may be prepared as a food, particularly a functional food. The functional food of the present invention is

It includes ingredients commonly added during food production, and includes, for example, proteins, carbohydrates, fats, nutrients and seasonings. For example, when manufactured as a drink, natural carbohydrates or flavoring agents may be included as additional ingredients in addition to the active ingredient. The natural carbohydrates include monosaccharides (eg, glucose, fructose, etc.), disaccharides (eg, maltose, sucrose, etc.), oligosaccharides, polysaccharides (eg, dextrin, cyclodextrin, etc.) or sugar alcohols (eg, , xylitol, sorbitol, erythritol, etc.). As the flavoring agent, natural flavoring agents (eg, taumatine, stevia extract, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) may be used.

In addition to the health functional food composition, various nutrients, vitamins, electrolytes, flavoring agents, coloring agents, pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonic acid It may further contain a carbonation agent and the like used in beverages.

The present invention provides a composition capable of enhancing antibacterial, anti-inflammatory and antiviral activity.

The present invention provides a composition for enhancing the immune activity of the human body.

The present invention provides a food or health food composition with excellent palatability due to excellent flavor while enhancing antibacterial, anti-inflammatory, antiviral and immune activity.

1 is a graph measuring cell viability by a composition according to an embodiment of the present invention.
Figure 2 is a graph measuring the concentration of nitric oxide present in the culture solution treated with the composition according to an embodiment of the present invention.
3 is a graph measuring the concentration of TNF-α present in the culture medium treated with the composition according to an embodiment of the present invention.
4 is a graph of measuring the concentration of interleukin-12 present in the culture medium with the composition according to an embodiment of the present invention.

Hereinafter, embodiments of the present invention will be described in detail so that those of ordinary skill in the art can easily carry out the present invention. However, the present invention may be embodied in several different forms and is not limited to the embodiments described herein.

[Preparation Example: Preparation of Extract]

1. Preparation of ginseng extract

After washing and drying the obtained ginseng, it was cut to a certain size and ground. The pulverized product and purified water were put into a high-temperature and high-pressure reactor, extracted, cooled, and concentrated and filtered to prepare a ginseng extract (GE).

2. Preparation of other extracts

Through hot water extraction, thistle extract (PE1), sycamore extract (PE2), coltsfoot extract (PE3), burdock extract (PE4), Schisandra extract (F1), beetroot extract (F2), soothing extract (F3), dermis extract (F4), chrysanthemum extract (F5) and cinnamon extract (F6) were prepared.

3. Preparation of the composition

A composite composition was prepared by mixing PE1 to PE4, and F1 to F6 with respect to the ginseng extract (GE) according to the composition shown in Table 1 below.

MM1 MM2 MM3 MM4 MM5 MM6 MM7 MM8 MM9 MM10 GE 100 100 100 100 100 100 100 100 100 100 PE1 100 100 100 100 100 100 100 100 100 100 PE2 - 0.1 One 5 10 20 5 5 5 5 PE3 - 0.1 One 3 5 10 3 3 3 3 PE4 - 0.1 One 3 5 10 3 3 3 3 F1 - - - - - - 0.1 One 5 10 F2 - - - - - - 0.1 One 5 10 F3 - - - - - - 0.01 0.1 One 5 F4 - - - - - - 0.1 One 5 10 F5 - - - - - - 0.1 One 5 10 F6 - - - - - - 0.01 0.1 One 5

(Unit: parts by weight)

[Experimental Example 1: Cytotoxicity evaluation]

RAW 264.7 cell, a mouse macrophage cell line, was purchased from the Korea Cell Line Bank (KCLB) and used. The RAW 264.7 cells were cultured in an incubator controlled _{at 37° C. and 5% CO 2} using DMEM medium containing 10% FBS and 1% penicillin-streptomycin. After that, RAW 264.7 cells were aliquoted in a 96-well plate at ^{a concentration of 1X10 4} cells/well and cultured for 24 hours, and the samples were treated with each concentration (0, 50, 100, 150 ug/ml). As a positive control, 1 ug/mL of lipopolysaccharide (LPS) was treated, and after 24 hours of incubation, CCK-8 solution (10 mL/well) was added to each well and treated at 37 ° C for 2 hours to 450 nm. Absorbance was measured at wavelength using an ELISA reader (TECAN, Mnnedorf, Switzerland).

The results are shown in [Fig. 1] below. Referring to the following [Fig. 1], GE and MM1 to MM10 prepared according to the preparation example exhibited 100% or more viability at all concentrations compared to the normal control group (con), so it can be confirmed that there is no problem of cytotoxicity. .

[Experimental Example 2: Measurement of Nitric Oxide (NO) Production]

The production of nitric oxide is known as an important signaling substance that acts as a defense against tumor cells or intracellularly infected microorganisms in the immune system. Therefore, in order to confirm the immune-enhancing ability, griess reagent [1% (w/v) sulfanilamide in 5% (v/v) phosphoric acid and 0.1% (w/v) naphtylethylenediamine- hydrochloric acid HCl)] was used to measure the concentration of ^{NO 2 -} present in the cell culture medium. As the experimental method, RAW 264.7 cells were aliquoted in a 6-well plate at ^{a concentration of 5X10 5} cells/well and cultured for 24 hours. Each was treated, and the sample was treated with 0 ug/mL as a normal control, and lipopolysaccharide was treated with 1 ug/mL as a positive control, and cultured for 24 hours. The supernatant of the cell culture solution and the same amount of griess reagent were mixed, reacted at room temperature for 15 minutes, absorbance was measured at 540 nm, and the nitric oxide concentration was calculated and converted from a standard curve prepared using sodium nitrite.

The results are shown in [Fig. 2] below.

Referring to the following [Fig. 2], it was possible to confirm a significant increase in weight over the control group throughout the Examples. In addition, it was confirmed that less than 40% of nitric oxide was produced compared to the positive control group.

On the other hand, in the case of MM3, 4, and 5, a significant increase was confirmed, and in the case of MM8 and MM9, an additional synergistic effect was confirmed. The excessive production of nitric oxide causes systemic inflammation and has various negative effects on the body, but the production of an appropriate amount of nitric oxide is considered as an important factor of innate immunity, so according to MM3, 4, and 5, the immune function activity is excellent and In particular, it was confirmed that in the case of MM8 and 9, better immune function activity could be exhibited.

[Experimental Example 3: Measurement of TNF-α and IL-12 production ability]

The secretion amount of TNF-α and IL-12 in the cell culture was measured using an ELISA kit (BD Bioscience, San Diego, CA, USA). Each of the capture antibodies (anti-mouse TNF-α and IL-12) was dispensed in a 96-well plate and coated at 4 °C overnight. After washing with washing buffer (PBS containing 0.05% Tween-20), blocking was performed with phosphate-buffered saline (PBS) containing 10% FBS for 1 hour, followed by washing with washing buffer. In each microplate, 100 uL of the supernatant of the same cell culture medium as in which nitric oxide was measured was dispensed and reacted at room temperature for 2 hours, followed by washing with washing buffer. Each diluted biotinylated anti-mouse TNF-α and interleukin-6 detection antibody and streptavidin-horseradish peroxidase conjugate solution were added and reacted at room temperature for 1 hour. After washing the reacted plate with washing buffer, tetramethylbenzidine (TMB) substrate solution was added to develop color for 30 minutes in the dark, 1 M phosphoric acid was added to stop the color reaction, and absorbance was measured at 450 nm.

The results are shown in [Fig. 3] below. Referring to the following [Fig. 3], as a result of examining the immune activity for the inflammatory cytokine TNF-α production ability, the secretion of TNF-α by macrophage stimulation is increased in the case of MM3 to MM5. confirmed to do. In addition, in the case of MM8 and MM9, it can be confirmed that the secretion of TNF-α produced by the positive control (lipopolysaccharide) is more promoted.

In addition, referring to [Fig. 4], the production of IL-12, an immune mediator, was increased in a concentration-dependent manner in MM3 to MM5, and it was confirmed that an additional synergistic effect appeared in the case of MM8 and MM9. It can be understood that MM3, 4, 5, or MM8, 9 may play an important role in the immune response by increasing the production of interleukin-6 to differentiate B lymphocytes.

[Experimental Example 4: Measurement of antibacterial and antiviral activity]

The strains used in the experiment to evaluate the antimicrobial activity were Gram-positive bacteria, Staphylococcus aureus KCTC 3881, Bacillus cereus KCTC 1012, Bacillus subtilis KCTC 1022) was used, and E. coli (Escherichia coli KCTC 2441), Pseudomonas aeruginosa (Pseudomonas aeruginosa KCTC 1636), and Salmonella enterica (Salmonella enterica KCTC 1925) were used as gram-negative bacteria. Agar medium (nutrient agar) was used as a bacterial growth medium. Six-week-old male and female rats of the SD line were divided into 10 rats per group (5 males and females each) and classified into groups administered with each composition, each drug was dissolved in 0.5% MC solution (prepared for Yongsi), and then 1 Dosing twice orally was repeated for 13 weeks. A single dose was administered in an amount of 3.75 mg/kg to 5 mg/kg. Thereafter, mortality, general symptoms, weight change, and feed and water intake were observed. The antibacterial activity of each composition (GE, MM1 to MM10) was measured by disc diffusion assay (Collins et. al 1995) for each strain. The plate medium for antibacterial test was prepared by smearing each subcultured strain by 200 uL using a sterile cotton swab. After drying, the solvent was volatilized, followed by incubation for 24 hours at 30~37°C in close contact on a plate medium. were compared. As a control, ampicillin (10 ug/disc) was used.

On the other hand, to evaluate the antiviral activity, MDCK cells, porcine epidemic diarrhea virus (PEDV, KPED-9 strain) and Pseudorabies virus (PRV, YS400 strain) to culture influenza virus A (Influenza virus A PR8 strain) to culture For culturing Vero cells and bovine rotavirus (BRV, P44 strain), MA104 cells were used. For culturing these cells, minimal essential medium (MEM) (Gibco BRL, Grand Island, NY, USA), 10% fetal bovine Serum (FBS) and 0.01% antibiotic-antimycotic were used. In order to culture Influenza A virus (PR8 strain), PEDV (KPED-9 strain), and BRV (P44 strain), MEM with 2.5 and 10 ug/ml trypsin (trypsin, sigma) added and FBS-free was used, respectively. did.

Vero, MDCK, and MA-104 cells were seeded in a 96-well cell culture plate by 3X10 ⁴ and cultured for 20 hours. Dispense the virus and each composition (GE, MM1 to MM10). After 3 days, a 50% tissue culture infective dose (TCID50) was measured using the Reed and Muench method for the titer of the virus through CPE caused by the virus. In the case of PEDV, since CPE was not observed, the titer was measured by immunofluorescence assay (IFA). In this method, after 3 days of culture, cells are washed with PBS, cells are fixed with 70% acetone, anti-PEDV spike protein monoclonal antibody (3F12, Xenobiotac) is added, left for 1 hour, washed, and anti -mouse IgG-FITC antibody was added, left for 1 hour, washed, and virus infection was confirmed through a fluorescence microscope. And at the same time, only the virus was cultured in the untreated group without the addition of GE, MM1 to MM10, and the decrease in CPE and infectivity caused by the virus by each extract was compared.

The results are comprehensively shown in [Table 2] below. For objective comparison, it was converted into an exponent of 1 to 10 compared to the control group. In the following index, the higher the number, the better the antibacterial or antiviral activity.

GE MM1 MM2 MM3 MM4 MM5 MM6 MM7 MM8 MM9 MM10 antibacterial activity 3 3 3 5 5 5 3 6 7 8 4 antiviral activity 2 3 3 4 5 5 2 5 7 7 5

(Unit: Index)

Referring to [Table 2], it can be seen that the antibacterial and antiviral activity is increased in MM3 to MM5. In particular, in the case of MM8 and MM9, it can be confirmed that a slight synergistic activity appears due to the synergistic effect of the mutual combination of each composition.

[Experimental Example 5: Evaluation of palatability]

The characteristic bitter taste and flavor of ginseng are often a problem in the dissemination of ginseng products, especially among foreign consumers. Therefore, palatability evaluation was performed for the GE, MM1 to MM10. After manufacturing GE, MM1 to MM10 as tea, 10 adults who responded that they did not like the smell of ginseng in the preliminary survey were asked to taste the prepared tea and then evaluate the flavor and taste. was shown.

The evaluation score was requested to be evaluated on a scale of 1 to 10 points for each item, and the evaluation result was converted into an average score. In the index, the higher the number, the higher the degree of preference.

GE MM1 MM2 MM3 MM4 MM5 MM6 MM7 MM8 MM9 MM10 Comprehensive symbol map 1.2 1.6 1.4 1.7 1.9 2.1 2.2 2.3 7.4 6.1 3.4

(Unit: Index)

Referring to [Table 3], in the case of MM1 to MM7, it can be seen that, overall, the characteristic bitter taste of ginseng is not neutralized, and the flavor is almost maintained, thereby not showing excellent palatability. On the other hand, in the case of MM10, the preference level slightly increased, but the overall preference level was evaluated to be low.

On the other hand, in the case of MM8 and MM9, the unique flavor and bitterness of ginseng were significantly neutralized as each extract was mixed, and the overall flavor was harmonized, so it was evaluated to have excellent palatability including flavor and taste. Therefore, in the case of MM8 and MM9, it can be confirmed that the distribution can be made easier as a ginseng-related food or health functional food with higher palatability.

Although the preferred embodiment of the present invention has been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention as defined in the following claims are also provided. is within the scope of the

Claims (6)

ginseng extract and milk thistle extract;
It further contains a seaweed extract, coltsfoot extract and burdock extract,
The milk thistle extract is included in an amount of 1:1 with respect to the ginseng extract,
Based on 100 parts by weight of the milk thistle extract, 1 to 10 parts by weight of the seaweed extract, 1 to 5 parts by weight of the coltsfoot extract, and 1 to 5 parts by weight of the burdock extract
A food composition for improving immune function. delete According to claim 1,
A food composition for improving immune function, further comprising a Schisandra extract, a persimmon extract, a soothing extract, a dermis extract, a chrysanthemum extract, and a cinnamon extract. The method according to claim 3, wherein, based on 100 parts by weight of the milk thistle extract, 1 to 5 parts by weight of the Schisandra extract, 1 to 5 parts by weight of the perennial herb extract, 0.1 to 1 parts by weight of the soothing extract, and 1 to 5 parts by weight of the dermis extract. , A food composition for improving immune function, comprising 1 to 5 parts by weight of chrysanthemum extract and 0.1 to 1 part by weight of cinnamon extract. The food composition for improving immune function according to claim 1, wherein the ginseng extract is extracted using an extraction solvent selected from the group consisting of water, alcohols having 1 to 10 carbon atoms, and mixtures thereof. A health functional food comprising the food composition according to any one of claims 1 to 5.

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