KR102298748B1 - Method for aging white ginseng and cultured wild ginseng with enhanced ginsenoside content using Makgeolli - Google Patents

Method for aging white ginseng and cultured wild ginseng with enhanced ginsenoside content using Makgeolli Download PDF

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KR102298748B1
KR102298748B1 KR1020210077887A KR20210077887A KR102298748B1 KR 102298748 B1 KR102298748 B1 KR 102298748B1 KR 1020210077887 A KR1020210077887 A KR 1020210077887A KR 20210077887 A KR20210077887 A KR 20210077887A KR 102298748 B1 KR102298748 B1 KR 102298748B1
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ginseng
aged
aging
prepared
makgeolli
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KR102298748B9 (en
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최성근
이창순
전병선
장성수
공병만
이건희
전혜정
박재형
김소연
김한민
김다영
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대동고려삼 주식회사
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/03Products from fruits or vegetables; Preparation or treatment thereof consisting of whole pieces or fragments without mashing the original pieces
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20Removal of unwanted matter, e.g. deodorisation or detoxification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
    • C12G3/021Preparation of other alcoholic beverages by fermentation of botanical family Poaceae, e.g. wheat, millet, sorghum, barley, rye, or corn
    • C12G3/022Preparation of other alcoholic beverages by fermentation of botanical family Poaceae, e.g. wheat, millet, sorghum, barley, rye, or corn of botanical genus Oryza, e.g. rice
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12GWINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
    • C12G3/00Preparation of other alcoholic beverages
    • C12G3/02Preparation of other alcoholic beverages by fermentation
    • C12G3/026Preparation of other alcoholic beverages by fermentation with health-improving ingredients, e.g. flavonoids, flavones, polyphenols or polysaccharides, added before or during the fermentation stage; with flavouring ingredients added before or during the fermentation stage
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts
    • A23V2250/2124Ginseng
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/50Concentrating, enriching or enhancing in functional factors

Abstract

The present invention relates to a preparing method of aged ginseng with enhanced ginsenoside content comprising the following steps: immersing white ginseng or cultured wild ginseng roots in makgeolli; and taking out the immersed white ginseng or cultured wild ginseng roots and aging the same. The present invention also relates to the aged ginseng with enhanced ginsenoside content prepared by the method.

Description

막걸리를 이용하여 진세노사이드 함량이 증진된 백삼 및 산삼배양근의 숙성방법{Method for aging white ginseng and cultured wild ginseng with enhanced ginsenoside content using Makgeolli}Method for aging white ginseng and cultured wild ginseng with enhanced ginsenoside content using Makgeolli}

본 발명은 (1) 감잎, 메밀싹 및 매실 효소액을 혼합한 혼합물을 숙성시킨 후 여과하여 여과액을 제조하는 단계; (2) 쌀을 물에 수침한 후 물빼기를 실시하고, 찜통에 넣어 고두밥을 제조하는 단계; (3) 상기 (2)단계의 제조한 고두밥에 효모를 넣고 숙성시키는 단계; (4) 상기 (3)단계의 숙성시킨 혼합물에 누룩가루, 물 및 상기 (1)단계의 제조한 여과액을 넣고 혼합한 후 숙성시키고 여과하여 막걸리를 제조하는 단계; (5) 백삼 또는 산삼배양근을 상기 (4)단계의 제조한 막걸리에 침지하는 단계; 및 (6) 상기 (5)단계의 침지한 백삼 또는 산삼배양근을 꺼낸 후 숙성시키는 단계를 포함하여 제조하는 것을 특징으로 하는 진세노사이드 함량이 증진된 숙성삼의 제조방법 및 상기 방법으로 제조된 숙성삼에 관한 것이다.The present invention comprises the steps of (1) ripening a mixture of persimmon leaves, buckwheat sprouts and plum enzyme solution, followed by filtering to prepare a filtrate; (2) immersing the rice in water, then draining the water, and putting it in a steamer to prepare godubap; (3) adding yeast to the godubap prepared in step (2) and aging; (4) preparing makgeolli by adding yeast powder, water, and the filtrate prepared in step (1) to the aged mixture in step (3), mixing, and then aging and filtering; (5) immersing white ginseng or wild ginseng cultured root in the makgeolli prepared in step (4); and (6) a method for producing aged ginseng with enhanced ginsenoside content, characterized in that it comprises the step of taking out the soaked white ginseng or wild ginseng cultured root in step (5) and then aging it. It's about three.

인삼(Panax ginseng C. A. Meyer)은 오가피과(Araliaceae) 인삼속(Panax) 식물에 속하는 다년생 반음지성 숙근초로서, 수천여년 전부터 우리나라를 비롯하여 동양에서 건강증진 기능을 가진 전통적인 약재로 이용되고 있는 대표적인 식물이다. 인삼은 가공 형태에 따라 원형이 유지되는 1차 가공 형태인 백삼, 홍삼, 태극삼 등이 있으며, 인삼의 뿌리에는 사포닌, 지용성 성분, 다당체, 함질소화 화합물, 유리당, 유리산, 비타민, 무기성분 등의 주성분이 포함되어 있으며, 이 중 진세노사이드는 중요한 약리효과가 있다는 것이 밝혀졌다.Ginseng ( Panax ginseng CA Meyer) is a perennial semi-shade succulent root plant belonging to the genus Panax of the Araliaceae family. Ginseng includes white ginseng, red ginseng, and Taegeuk ginseng, which are primary processed types that maintain their original shape depending on the processing type. In the root of ginseng, saponins, fat-soluble components, polysaccharides, nitrogen-containing compounds, free sugars, free acids, vitamins, inorganic components, etc. It has been found that ginsenosides have important pharmacological effects among them.

백삼은 통상 4∼6년근의 수삼을 박피하거나 혹은 박피하지 않은 상태에서 자연건조시키거나 또는 60℃ 이하로 열풍건조시켜 수분함량이 14% 이하가 되도록 한 것으로서, 박피한 것을 백삼이라고 하고 박피하지 않은 상태의 것을 피부백삼이라고 한다. 백삼은 건조시킨 형태에 따라 직삼, 반곡삼, 곡삼 등으로 구분되고, 제품의 색은 유백색이나 연노랑색을 나타낸다.White ginseng is usually fresh ginseng 4 to 6 years old, either peeled or dried naturally without peeling, or dried in hot air at 60°C or lower so that the moisture content is 14% or less. It is called skin white ginseng. White ginseng is divided into straight ginseng, bangok ginseng, and grain ginseng according to the dried form, and the product color is milky white or pale yellow.

산삼배양근은 야생산삼을 모체로 하여 이를 동일하게 조직 배양하는 수경(양액) 재배(배양) 농산물인데, 기본적으로 20~30일 동안 사전배양(Seed 배양)한 후 40~120일 동안 본배양(확대배양)을 통해 생산(재배)하는 농산물로서 농약 등 위해 성분이 근본적으로 배제된 친환경적인 방법으로 생산되고, 노지에서 재배하는 인삼 등과 근본적으로 다른 특징이 있다. 산삼배양근은 배양조건과 배양기간을 조정함으로써 원하는 두께(직경, 굵기)와 크기의 배양근을 맞춤형으로 생산할 수 있어, 이를 통해 식품첨가물로서 산삼배양근의 활용성과 효용성이 극대화된다.Wild ginseng cultured root is a hydroponic (nutrient solution) cultivated (cultured) agricultural product that uses wild ginseng as a parent and tissue culture it in the same way. As an agricultural product produced (cultivated) through cultivation), it is produced by an eco-friendly method that fundamentally excludes harmful ingredients such as pesticides, and has fundamentally different characteristics from ginseng grown in the open field. Cultured wild ginseng roots can be customized to the desired thickness (diameter, thickness) and size by adjusting the culture conditions and culture period, thereby maximizing the utility and utility of cultured wild ginseng roots as a food additive.

한국등록특허 제1993623호에는 숙성 인삼의 제조방법이 개시되어 있고, 한국등록특허 제2074810호에는 가공 인삼의 제조방법이 개시되어 있으나, 본 발명의 막걸리를 이용하여 진세노사이드 함량이 증진된 백삼 및 산삼배양근의 숙성방법과는 상이하다.Korean Patent No. 1993623 discloses a method for producing aged ginseng, and Korean Patent No. 2074810 discloses a method for producing processed ginseng. It is different from the aging method of wild ginseng cultured root.

본 발명은 상기와 같은 요구에 의해 도출된 것으로서, 본 발명의 목적은 백삼 또는 산삼배양근의 약리적 효능을 높이면서 쓴맛은 감소시키고 부드러운 맛과 향을 증진시키기 위해, 막걸리 제조, 침지 및 숙성 등의 제조조건을 최적화하여 품질 및 기호도가 증진된 숙성 백삼 또는 숙성 산삼배양근의 제조방법을 제공하는 데 있다.The present invention has been derived from the above needs, and an object of the present invention is to increase the pharmacological efficacy of white ginseng or wild ginseng cultured root, while reducing bitterness and enhancing soft taste and flavor, manufacturing makgeolli, immersion and aging, etc. An object of the present invention is to provide a method for producing aged white ginseng or aged wild ginseng cultured root with improved quality and preference by optimizing conditions.

상기 과제를 해결하기 위해, 본 발명은 (1) 감잎, 메밀싹 및 매실 효소액을 혼합한 혼합물을 숙성시킨 후 여과하여 여과액을 제조하는 단계; (2) 쌀을 물에 수침한 후 물빼기를 실시하고, 찜통에 넣어 고두밥을 제조하는 단계; (3) 상기 (2)단계의 제조한 고두밥에 효모를 넣고 숙성시키는 단계; (4) 상기 (3)단계의 숙성시킨 혼합물에 누룩가루, 물 및 상기 (1)단계의 제조한 여과액을 넣고 혼합한 후 숙성시키고 여과하여 막걸리를 제조하는 단계; (5) 백삼 또는 산삼배양근을 상기 (4)단계의 제조한 막걸리에 침지하는 단계; 및 (6) 상기 (5)단계의 침지한 백삼 또는 산삼배양근을 꺼낸 후 숙성시키는 단계를 포함하여 제조하는 것을 특징으로 하는 진세노사이드 함량이 증진된 숙성삼의 제조방법을 제공한다.In order to solve the above problems, the present invention comprises the steps of (1) aging a mixture of persimmon leaves, buckwheat sprouts and plum enzyme solution and then filtering it to prepare a filtrate; (2) immersing the rice in water, then draining the water, and putting it in a steamer to prepare godubap; (3) adding yeast to the godubap prepared in step (2) and aging; (4) preparing makgeolli by adding yeast powder, water, and the filtrate prepared in step (1) to the aged mixture in step (3), mixing, and then aging and filtering; (5) immersing white ginseng or wild ginseng cultured root in the makgeolli prepared in step (4); And (6) provides a method for producing aged ginseng with enhanced ginsenoside content, characterized in that it comprises the step of aging after taking out the soaked white ginseng or wild ginseng cultured root in step (5).

또한, 본 발명은 상기 방법으로 제조된 진세노사이드 함량이 증진된 숙성삼을 제공한다.In addition, the present invention provides an enhanced ginsenoside content aged ginseng prepared by the above method.

본 발명은 백삼 및 산삼배양근의 막걸리 숙성을 통해 특정 진세노사이드 함량 증가하여 건강 기능성 식품 또는 다양한 분야에서 활용이 가능하며, 종래의 백삼 및 산삼배양근 가공식품과는 차별화된 고품질의 제품의 생산이 가능할 것으로 기대된다.The present invention can be used in health functional food or various fields by increasing the specific ginsenoside content through the fermentation of makgeolli of white ginseng and wild ginseng cultured root, and it is possible to produce high-quality products differentiated from conventional white ginseng and wild ginseng cultured root processed food. is expected to

도 1은 막걸리에 침지한 백삼 또는 산삼배양근을 꺼낸 후 항온항습기에서 숙성시키는 사진을 보여준다. 1 shows a photograph of taking out white ginseng or wild ginseng cultured root immersed in makgeolli and then aging it in a thermo-hygrostat.

본 발명의 목적을 달성하기 위하여, 본 발명은In order to achieve the object of the present invention, the present invention

(1) 감잎, 메밀싹 및 매실 효소액을 혼합한 혼합물을 숙성시킨 후 여과하여 여과액을 제조하는 단계;(1) aging a mixture of persimmon leaves, buckwheat sprouts and plum enzyme solution, followed by filtering to prepare a filtrate;

(2) 쌀을 물에 수침한 후 물빼기를 실시하고, 찜통에 넣어 고두밥을 제조하는 단계;(2) immersing the rice in water, then draining the water, and putting it in a steamer to prepare godubap;

(3) 상기 (2)단계의 제조한 고두밥에 효모를 넣고 숙성시키는 단계;(3) adding yeast to the godubap prepared in step (2) and aging;

(4) 상기 (3)단계의 숙성시킨 혼합물에 누룩가루, 물 및 상기 (1)단계의 제조한 여과액을 넣고 혼합한 후 숙성시키고 여과하여 막걸리를 제조하는 단계;(4) preparing makgeolli by adding yeast powder, water, and the filtrate prepared in step (1) to the aged mixture in step (3), mixing, and then aging and filtering;

(5) 백삼 또는 산삼배양근을 상기 (4)단계의 제조한 막걸리에 침지하는 단계; 및(5) immersing white ginseng or wild ginseng cultured root in the makgeolli prepared in step (4); and

(6) 상기 (5)단계의 침지한 백삼 또는 산삼배양근을 꺼낸 후 숙성시키는 단계를 포함하여 제조하는 것을 특징으로 하는 진세노사이드 함량이 증진된 숙성삼의 제조방법을 제공한다.(6) It provides a method for producing aged ginseng with enhanced ginsenoside content, characterized in that it comprises the step of aging after taking out the soaked white ginseng or wild ginseng cultured root in step (5).

본 발명의 숙성삼의 제조방법에서, 상기 (1)단계의 여과액은 바람직하게는 감잎, 메밀싹 및 매실 효소액을 2.7~3.3:1.8~2.2:4.5~5.5 중량비율로 혼합한 혼합물을 20~30℃에서 1~3개월 동안 숙성시킨 후 여과할 수 있으며, 더욱 바람직하게는 감잎, 메밀싹 및 매실 효소액을 3:2:5 중량비율로 혼합한 혼합물을 25℃에서 2개월 동안 숙성시킨 후 여과하여 제조할 수 있다. 본 발명자는 예비실험에서 상기 여과액을 첨가하지 않거나, 상기 재료 일부만 사용한 여과액을 사용하여 막걸리를 제조하는 것에 비해, 상기와 같은 방법으로 제조된 여과액을 사용하여 막걸리를 제조하는 것이 숙성삼의 진세노사이드 함량을 더욱 증진시킬 수 있었다.In the method for producing aged ginseng of the present invention, the filtrate of step (1) is preferably a mixture of persimmon leaf, buckwheat sprout and plum enzyme solution in a weight ratio of 2.7-3.3:1.8-2.2:4.5-5.5, 20- After aging for 1 to 3 months at 30°C, it can be filtered, and more preferably, a mixture of persimmon leaf, buckwheat sprout, and plum enzyme solution in a 3:2:5 weight ratio is aged at 25°C for 2 months and then filtered can be manufactured. In the preliminary experiment, the present inventors found that manufacturing makgeolli using the filtrate prepared in the same manner as described above is better than preparing makgeolli using the filtrate using only a portion of the material or not adding the filtrate in the preliminary experiment. It was possible to further enhance the ginsenoside content.

상기 매실 효소액은 바람직하게는 매실과 설탕을 0.8~1.2:0.8~1.2 중량비율로 혼합한 후 25~30℃에서 1~3개월 동안 숙성시킨 후 여과하여 제조할 수 있으며, 더욱 바람직하게는 매실과 설탕을 1:1 중량비율로 혼합한 후 25~30℃에서 2개월 동안 숙성시킨 후 여과하여 제조할 수 있다.The plum enzyme solution is preferably mixed with plum and sugar in a weight ratio of 0.8 to 1.2: 0.8 to 1.2, aged at 25 to 30° C. for 1-3 months, and then filtered to prepare, more preferably plum and It can be prepared by mixing sugar in a 1:1 weight ratio, aging it at 25-30° C. for 2 months, and then filtering it.

또한, 본 발명의 숙성삼의 제조방법에서, 상기 (2)단계의 고두밥은 바람직하게는 쌀 0.8~1.2 kg를 물에 3~5시간 동안 수침한 후 물빼기를 실시하고, 찜통에 넣어 제조할 수 있으며, 더욱 바람직하게는 쌀 1 kg를 물에 4시간 동안 수침한 후 물빼기를 실시하고, 찜통에 넣어 제조할 수 있다. 상기와 같은 조건으로 고두밥을 제조하는 것이 쌀의 전분을 충분히 호화시켜 이후 단계인 숙성단계에서 숙성을 용이하게 할 수 있었다.In addition, in the manufacturing method of aged ginseng of the present invention, the godubap in step (2) is preferably prepared by immersing 0.8 to 1.2 kg of rice in water for 3 to 5 hours, then draining the water, and putting it in a steamer. More preferably, 1 kg of rice is immersed in water for 4 hours and then drained, and it can be prepared by putting it in a steamer. Preparation of godubap under the same conditions as above made it possible to sufficiently gelatinize the starch of the rice, thereby facilitating the aging in the later stage, the ripening stage.

또한, 본 발명의 숙성삼의 제조방법에서, 상기 (3)단계는 바람직하게는 고두밥에 효모 45~55 g을 넣고 20~30℃에서 16~20시간 동안 숙성시킬 수 있으며, 더욱 바람직하게는 고두밥에 효모 50 g을 넣고 25℃에서 18시간 동안 숙성시킬 수 있다. 상기와 같은 조건으로 숙성시키는 것이 이취의 발생은 최소화하면서 효모를 충분히 확대 배양시켜 막걸리 제조에 적합한 혼합물로 제조할 수 있었다.In addition, in the method for producing aged ginseng of the present invention, the step (3) may include adding 45 to 55 g of yeast to godubap and aging it at 20 to 30° C. for 16 to 20 hours, more preferably godubap. You can put 50 g of yeast in the fermented at 25 ℃ for 18 hours. Aging under the above conditions minimized the occurrence of off-flavor and sufficiently expanded the yeast to prepare a mixture suitable for makgeolli production.

또한, 본 발명의 숙성삼의 제조방법에서, 상기 (4)단계의 막걸리는 바람직하게는 숙성시킨 혼합물에 누룩가루 450~550 g, 물 1000~1400 mL 및 여과액 0.8~1.2 L를 넣고 혼합한 후 25~35℃에서 5~7일 동안 숙성시키고 여과하여 제조할 수 있으며, 더욱 바람직하게는 숙성시킨 혼합물에 누룩가루 500 g, 물 1200 mL 및 여과액 1 L를 넣고 혼합한 후 25~35℃에서 5~7일 동안 숙성시키고 여과하여 제조할 수 있다. 상기와 같은 재료 종류, 배합비 및 숙성조건으로 숙성시켜 막걸리를 제조하는 것이 텁텁한 맛은 감소하고 부드럽고 개운한 맛을 지니면서, 진세노사이드 함량을 더욱 향상시킬 수 있는 삼 전처리에 적합한 막걸리로 제조할 수 있었다.In addition, in the method for producing aged ginseng of the present invention, the makgeolli of step (4) is preferably mixed with 450 to 550 g of yeast powder, 1000 to 1400 mL of water and 0.8 to 1.2 L of filtrate in the aged mixture. Then, it can be aged at 25-35°C for 5-7 days and filtered, and more preferably, 500 g of yeast powder, 1200 mL of water, and 1 L of filtrate are added to the aged mixture and mixed at 25-35°C. It can be prepared by aging for 5 to 7 days and filtration. It was possible to prepare makgeolli suitable for ginseng pretreatment that can further improve the ginsenoside content while reducing the bitter taste and having a soft and refreshing taste by aging with the same material types, blending ratio and aging conditions as above. .

또한, 본 발명의 숙성삼의 제조방법에서, 상기 (5)단계의 침지는 바람직하게는 백삼 또는 산삼배양근을 막걸리에 25~35℃에서 20~28시간 동안 침지할 수 있으며, 더욱 바람직하게는 백삼 또는 산삼배양근을 막걸리에 30℃에서 24시간 동안 침지할 수 있다. 상기와 같은 조건으로 침지하는 것이 삼 특유 쓴맛은 저감시키면서 막걸리와 풍미가 잘 어우러지고 진세노사이드 증진 효과가 우수하도록 삼을 전처리할 수 있었다.In addition, in the method for producing aged ginseng of the present invention, the immersion in step (5) may include immersing white ginseng or wild ginseng cultured root in makgeolli for 20 to 28 hours, more preferably white ginseng. Alternatively, wild ginseng cultured roots can be immersed in makgeolli at 30° C. for 24 hours. Immersion under the same conditions as described above was able to pre-treat ginseng so that the characteristic bitter taste of ginseng was reduced, the flavor was well combined with makgeolli, and the ginsenoside enhancing effect was excellent.

또한, 본 발명의 숙성삼의 제조방법에서, 상기 (6)단계의 숙성은 바람직하게는 33~40℃에서 2~8일 동안 숙성시킬 수 있으며, 더욱 바람직하게는 37℃에서 3~7일 동안 숙성시킬 수 있다. 상기와 같은 조건으로 숙성시키는 것이 삼의 부드러운 맛과 풍미는 향상시키면서 특정 진세노사이드 함량을 높일 수 있었다.In addition, in the manufacturing method of the aged ginseng of the present invention, the aging in step (6) may preferably be aged at 33 to 40 ° C. for 2 to 8 days, more preferably at 37 ° C. for 3 to 7 days. can be aged. Aging under the above conditions was able to increase the specific ginsenoside content while improving the soft taste and flavor of ginseng.

또한, 본 발명의 숙성삼의 제조방법에서, 상기 진세노사이드는 숙성삼이 숙성 백삼일 경우 Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, Rb3 및 Rd로 이루어진 군으로부터 선택되는 하나 이상의 진세노사이드일 수 있고, 상기 숙성삼이 숙성 산삼배양근일 경우 Rg1, Re, Rf, Rg2, Rc, Rd 및 F2로 이루어진 군으로부터 선택되는 하나 이상의 진세노사이드일 수 있으나, 이에 제한되지 않는다.In addition, in the method for producing aged ginseng of the present invention, the ginsenoside is one or more ginsenosides selected from the group consisting of Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, Rb3 and Rd when the aged ginseng is aged white ginseng. may be, and when the aged ginseng is aged wild ginseng cultured root, it may be one or more ginsenosides selected from the group consisting of Rg1, Re, Rf, Rg2, Rc, Rd and F2, but is not limited thereto.

본 발명의 숙성삼의 제조방법에서, 상기 숙성삼이 숙성 백삼일 경우, 보다 구체적으로는In the method for producing aged ginseng of the present invention, when the aged ginseng is aged white ginseng, more specifically,

(1) 감잎, 메밀싹 및 매실 효소액을 2.7~3.3:1.8~2.2:4.5~5.5 중량비율로 혼합한 혼합물을 20~30℃에서 1~3개월 동안 숙성시킨 후 여과하여 여과액을 제조하는 단계;(1) A mixture of persimmon leaf, buckwheat sprout and plum enzyme solution in a weight ratio of 2.7~3.3:1.8~2.2:4.5~5.5 is aged at 20~30℃ for 1~3 months and filtered to prepare a filtrate ;

(2) 쌀 0.8~1.2 kg를 물에 3~5시간 동안 수침한 후 물빼기를 실시하고, 찜통에 넣어 고두밥을 제조하는 단계;(2) immersing 0.8~1.2 kg of rice in water for 3 to 5 hours, then draining the water, and putting it in a steamer to prepare godubap;

(3) 상기 (2)단계의 제조한 고두밥에 효모 45~55 g을 넣고 20~30℃에서 16~20시간 동안 숙성시키는 단계;(3) adding 45 to 55 g of yeast to the godubap prepared in step (2) and aging at 20 to 30° C. for 16 to 20 hours;

(4) 상기 (3)단계의 숙성시킨 혼합물에 누룩가루 450~550 g, 물 1000~1400 mL 및 상기 (1)단계의 제조한 여과액 0.8~1.2 L를 넣고 혼합한 후 25~35℃에서 5~7일 동안 숙성시키고 여과하여 막걸리를 제조하는 단계;(4) Add 450 to 550 g of yeast powder, 1000 to 1400 mL of water, and 0.8 to 1.2 L of the filtrate prepared in step (1) to the aged mixture of step (3), mix, and then at 25 to 35° C. Aging for 5-7 days and filtering to prepare makgeolli;

(5) 백삼을 상기 (4)단계의 제조한 막걸리에 25~35℃에서 20~28시간 동안 침지하는 단계; 및(5) immersing white ginseng in the makgeolli prepared in step (4) at 25-35° C. for 20-28 hours; and

(6) 상기 (5)단계의 침지한 백삼을 꺼낸 후 33~40℃에서 2~8일 동안 숙성시키는 단계를 포함할 수 있으며,(6) may include the step of taking out the immersed white ginseng in step (5) and then aging it at 33 to 40 ° C for 2 to 8 days,

더욱 구체적으로는more specifically

(1) 감잎, 메밀싹 및 매실 효소액을 3:2:5 중량비율로 혼합한 혼합물을 25℃에서 2개월 동안 숙성시킨 후 여과하여 여과액을 제조하는 단계;(1) A mixture of persimmon leaves, buckwheat sprouts and plum enzyme solution in a 3:2:5 weight ratio was aged at 25° C. for 2 months and then filtered to prepare a filtrate;

(2) 쌀 1 kg를 물에 4시간 동안 수침한 후 물빼기를 실시하고, 찜통에 넣어 고두밥을 제조하는 단계;(2) immersing 1 kg of rice in water for 4 hours, then draining the water, and putting it in a steamer to prepare godubap;

(3) 상기 (2)단계의 제조한 고두밥에 효모 50 g을 넣고 25℃에서 18시간 동안 숙성시키는 단계;(3) adding 50 g of yeast to the godubap prepared in step (2) and aging at 25° C. for 18 hours;

(4) 상기 (3)단계의 숙성시킨 혼합물에 누룩가루 500 g, 물 1200 mL 및 상기 (1)단계의 제조한 여과액 1 L를 넣고 혼합한 후 30℃에서 6일 동안 숙성시키고 여과하여 막걸리를 제조하는 단계;(4) Put 500 g of yeast powder, 1200 mL of water, and 1 L of the filtrate prepared in step (1) to the aged mixture in step (3), mix, and then aged at 30° C. for 6 days, filtered and filtered to make makgeolli preparing a;

(5) 백삼을 상기 (4)단계의 제조한 막걸리에 30℃에서 24시간 동안 침지하는 단계; 및(5) immersing white ginseng in the makgeolli prepared in step (4) at 30° C. for 24 hours; and

(6) 상기 (5)단계의 침지한 백삼을 꺼낸 후 37℃에서 3~7일 동안 숙성시킬 수 있다.(6) After taking out the immersed white ginseng in step (5), it can be aged at 37° C. for 3 to 7 days.

또한, 본 발명의 숙성삼의 제조방법에서, 상기 숙성삼이 숙성 산삼배양근일 경우, 보다 구체적으로는In addition, in the method for producing aged ginseng of the present invention, when the aged ginseng is aged wild ginseng cultured root, more specifically,

(1) 감잎, 메밀싹 및 매실 효소액을 2.7~3.3:1.8~2.2:4.5~5.5 중량비율로 혼합한 혼합물을 20~30℃에서 1~3개월 동안 숙성시킨 후 여과하여 여과액을 제조하는 단계;(1) A mixture of persimmon leaf, buckwheat sprout and plum enzyme solution in a weight ratio of 2.7~3.3:1.8~2.2:4.5~5.5 is aged at 20~30℃ for 1~3 months and filtered to prepare a filtrate ;

(2) 쌀 0.8~1.2 kg를 물에 3~5시간 동안 수침한 후 물빼기를 실시하고, 찜통에 넣어 고두밥을 제조하는 단계;(2) immersing 0.8~1.2 kg of rice in water for 3 to 5 hours, then draining the water, and putting it in a steamer to prepare godubap;

(3) 상기 (2)단계의 제조한 고두밥에 효모 45~55 g을 넣고 20~30℃에서 16~20시간 동안 숙성시키는 단계;(3) adding 45 to 55 g of yeast to the godubap prepared in step (2) and aging at 20 to 30° C. for 16 to 20 hours;

(4) 상기 (3)단계의 숙성시킨 혼합물에 누룩가루 450~550 g, 물 1000~1400 mL 및 상기 (1)단계의 제조한 여과액 0.8~1.2 L를 넣고 혼합한 후 25~35℃에서 5~7일 동안 숙성시키고 여과하여 막걸리를 제조하는 단계;(4) Add 450 to 550 g of yeast powder, 1000 to 1400 mL of water, and 0.8 to 1.2 L of the filtrate prepared in step (1) to the aged mixture of step (3), mix, and then at 25 to 35° C. Aging for 5-7 days and filtering to prepare makgeolli;

(5) 산삼배양근을 상기 (4)단계의 제조한 막걸리에 25~35℃에서 20~28시간 동안 침지하는 단계; 및(5) immersing the cultured wild ginseng root in the makgeolli prepared in step (4) at 25-35° C. for 20-28 hours; and

(6) 상기 (5)단계의 침지한 산삼배양근을 꺼낸 후 33~40℃에서 6~8일 동안 숙성시키는 단계를 포함할 수 있으며,(6) may include the step of taking out the immersed wild ginseng cultured root in step (5) and then aging it at 33-40 ° C. for 6-8 days,

더욱 구체적으로는more specifically

(1) 감잎, 메밀싹 및 매실 효소액을 3:2:5 중량비율로 혼합한 혼합물을 25℃에서 2개월 동안 숙성시킨 후 여과하여 여과액을 제조하는 단계;(1) A mixture of persimmon leaves, buckwheat sprouts and plum enzyme solution in a 3:2:5 weight ratio was aged at 25° C. for 2 months and then filtered to prepare a filtrate;

(2) 쌀 1 kg를 물에 4시간 동안 수침한 후 물빼기를 실시하고, 찜통에 넣어 고두밥을 제조하는 단계;(2) immersing 1 kg of rice in water for 4 hours, then draining the water, and putting it in a steamer to prepare godubap;

(3) 상기 (2)단계의 제조한 고두밥에 효모 50 g을 넣고 25℃에서 18시간 동안 숙성시키는 단계;(3) adding 50 g of yeast to the godubap prepared in step (2) and aging at 25° C. for 18 hours;

(4) 상기 (3)단계의 숙성시킨 혼합물에 누룩가루 500 g, 물 1200 mL 및 상기 (1)단계의 제조한 여과액 1 L를 넣고 혼합한 후 30℃에서 6일 동안 숙성시키고 여과하여 막걸리를 제조하는 단계;(4) Put 500 g of yeast powder, 1200 mL of water, and 1 L of the filtrate prepared in step (1) to the aged mixture in step (3), mix, and then aged at 30° C. for 6 days, filtered and filtered to make makgeolli preparing a;

(5) 산삼배양근을 상기 (4)단계의 제조한 막걸리에 30℃에서 24시간 동안 침지하는 단계; 및(5) immersing the cultured wild ginseng root in the makgeolli prepared in step (4) at 30° C. for 24 hours; and

(6) 상기 (5)단계의 침지한 산삼배양근을 꺼낸 후 37℃에서 7일 동안 숙성시키는 단계를 포함할 수 있다.(6) It may include the step of taking out the immersed wild ginseng cultured root in step (5) and then aging it at 37° C. for 7 days.

본 발명은 또한, 상기 방법으로 제조된 진세노사이드 함량이 증진된 숙성삼을 제공한다.The present invention also provides an enhanced ginsenoside content aged ginseng prepared by the above method.

이하, 본 발명을 실시예에 의해 상세히 설명한다. 단, 하기 실시예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예에 한정되는 것은 아니다.Hereinafter, the present invention will be described in detail by way of Examples. However, the following examples are merely illustrative of the present invention, and the content of the present invention is not limited to the following examples.

제조예 1. 숙성 백삼근Preparation Example 1. Aged white ginseng root

(1) 감잎, 메밀싹 및 매실 효소액을 3:2:5 중량비율로 혼합한 혼합물을 25℃에서 2개월 동안 숙성시킨 후 여과하여 여과액을 제조하였다.(1) A mixture of persimmon leaf, buckwheat sprout, and plum enzyme solution in a 3:2:5 weight ratio was aged at 25° C. for 2 months and filtered to prepare a filtrate.

(2) 멥쌀 1 kg를 물에 4시간 동안 수침한 후 물빼기를 실시하고, 찜통에 넣어 고두밥을 제조하였다.(2) 1 kg of non-glutinous rice was immersed in water for 4 hours, drained, and put in a steamer to prepare godubap.

(3) 상기 (2)단계의 제조한 고두밥에 효모(Fresh yeast, Ottogi, Anyang, Korea) 50 g을 넣고 25℃에서 18시간 동안 숙성시켰다.(3) 50 g of yeast (Fresh yeast, Ottogi, Anyang, Korea) was added to the godubap prepared in step (2) and aged at 25° C. for 18 hours.

(4) 상기 (3)단계의 숙성시킨 혼합물에 누룩가루 500 g, 물 1200 mL 및 상기 (1)단계의 제조한 여과액 1 L를 넣고 혼합한 후 30℃에서 6일 동안 숙성시키고 여과하여 막걸리를 제조하였다.(4) Put 500 g of yeast powder, 1200 mL of water, and 1 L of the filtrate prepared in step (1) to the aged mixture in step (3), mix, and then aged at 30° C. for 6 days, filtered and filtered to make makgeolli was prepared.

(5) 백삼 주근(동체)을 1.5~2.5 cm 크기로 절단한 후 상기 (4)단계의 제조한 막걸리에 30℃에서 24시간 동안 침지하였다.(5) After cutting the main root of white ginseng (body) to 1.5-2.5 cm in size, it was immersed in the makgeolli prepared in step (4) at 30° C. for 24 hours.

(6) 상기 (5)단계의 침지한 백삼을 꺼낸 후 항온항습기에서 37℃의 온도 및 60% 습도에서 7일 동안 숙성시켰다.(6) After taking out the immersed white ginseng in step (5), it was aged for 7 days at a temperature of 37°C and a humidity of 60% in a thermo-hygrostat.

제조예 2. 숙성 백삼미Preparation Example 2. Aged white ginseng rice

상기 제조예 1의 방법으로 제조하되, 상기 (5)단계에서 백삼 주근 대신 백삼 세미(잔뿌리)를 이용하여 숙성 백삼미를 제조하였다.It was prepared by the method of Preparation Example 1, but aged white ginseng rice was prepared using white ginseng semi (fine root) instead of white ginseng main root in step (5).

제조예 3. 숙성 산삼배양근Preparation Example 3. Aged wild ginseng cultured root

상기 제조예 1의 방법으로 제조하되, 상기 (5)단계에서 백삼 주근 대신 산삼배양근을 이용하여 숙성 산삼배양근을 제조하였다.It was prepared by the method of Preparation Example 1, but in step (5), cultured wild ginseng roots were used instead of the main roots of white ginseng to prepare mature wild ginseng roots.

비교예 1. 숙성 백삼근Comparative Example 1. Aged white ginseng root

(1) 멥쌀 1 kg를 물에 4시간 동안 수침한 후 물빼기를 실시하고, 찜통에 넣어 고두밥을 제조하였다.(1) 1 kg of non-glutinous rice was immersed in water for 4 hours, drained, and put in a steamer to prepare godubap.

(2) 상기 (1)단계의 제조한 고두밥에 효모(Fresh yeast, Ottogi, Anyang, Korea) 50 g을 넣고 25℃에서 18시간 동안 숙성시켰다.(2) 50 g of yeast (Fresh yeast, Ottogi, Anyang, Korea) was added to the godubap prepared in step (1) and aged at 25° C. for 18 hours.

(3) 상기 (2)단계의 숙성시킨 혼합물에 누룩가루 500 g 및 물 1200 mL를 넣고 혼합한 후 30℃에서 6일 동안 숙성시키고 여과하여 막걸리를 제조하였다.(3) 500 g of yeast powder and 1200 mL of water were added to the aged mixture in step (2), mixed, aged at 30° C. for 6 days, and filtered to prepare makgeolli.

(4) 백삼 주근(동체)을 1.5~2.5 cm 크기로 절단한 후 상기 (3)단계의 제조한 막걸리에 30℃에서 24시간 동안 침지하였다.(4) After cutting the main root of white ginseng (body) to 1.5-2.5 cm in size, it was immersed in the makgeolli prepared in step (3) at 30° C. for 24 hours.

(5) 상기 (4)단계의 침지한 백삼을 꺼낸 후 항온항습기에서 37℃의 온도 및 60% 습도에서 7일 동안 숙성시켰다.(5) After taking out the white ginseng immersed in step (4), it was aged for 7 days at a temperature of 37° C. and a humidity of 60% in a thermo-hygrostat.

비교예 2. 숙성 백삼미Comparative Example 2. Aged white ginseng rice

상기 비교예 1의 방법으로 제조하되, 상기 (4)단계에서 백삼 주근 대신 백삼 세미(잔뿌리)를 이용하여 숙성 백삼미를 제조하였다.It was prepared by the method of Comparative Example 1, but aged white ginseng rice was prepared using white ginseng semi (fine root) instead of white ginseng main root in step (4).

비교예 3. 숙성 산삼배양근Comparative Example 3. Aged wild ginseng cultured root

상기 비교예 1의 방법으로 제조하되, 상기 (4)단계에서 백삼 주근 대신 산삼배양근을 이용하여 숙성 산삼배양근을 제조하였다.It was prepared by the method of Comparative Example 1, but in step (4), cultured wild ginseng roots were used instead of white ginseng main roots to prepare mature wild ginseng roots.

1. 진세노사이드 함량 측정 방법1. Method for measuring ginsenoside content

진세노사이드의 함량 분석은 UVD(Ultra Visible Detector)가 장착된 HPLC(High Performance Liquid Chromatography)로 분석하였고, 분석조건은 하기 표 1과 같고, 함량 계산식은 하기 식과 같다.The content analysis of ginsenosides was analyzed by High Performance Liquid Chromatography (HPLC) equipped with UVD (Ultra Visible Detector), the analysis conditions are shown in Table 1 below, and the content calculation formula is as follows.

진세노사이드 함량(㎎/g) = S × (a×b)/시료채취량(g)Ginsenoside content (mg/g) = S × (a×b)/sample amount (g)

S: 시험용액 중 개별 진세노사이드 농도(㎎/㎖)S: Individual ginsenoside concentration in the test solution (mg/ml)

a: 시험용액의 전량(㎖)a: Total amount of test solution (ml)

b: 희석배수b: dilution factor

진세노사이드 분석조건Ginsenoside analysis conditions 장비equipment HPLC Agilent 1260 seriesHPLC Agilent 1260 series 검출기detector DAD detector(203 nm)DAD detector (203 nm) 컬럼column Prontosil 120-5-C18-ace-EPS
(4.6 mm×250 mm, 5.0 ㎛)
Prontosil 120-5-C18-ace-EPS
(4.6 mm×250 mm, 5.0 μm)
컬럼온도column temperature 40℃40℃ 이동상mobile phase Acetonitrile:D.W. (gradient)Acetonitrile: D.W. (gradient) 유속flow rate 1.0 ㎖/min1.0 ml/min 주입 용량infusion dose 10 ㎕10 μl

실시예 1. 백삼 주근의 숙성 전후 진세노사이드 함량 및 증가율(%)Example 1. Ginsenoside content and increase rate (%) before and after ripening of white ginseng main root

백삼 주근의 숙성 전(대조구)과 후 진세노사이드 함량을 비교한 결과는 하기 표 2와 같다. 백삼근을 숙성하였을 때 진세노사이드가 전체적으로 증가하였다. 인삼의 지표성분 함량은 진세노사이드 Rg1과 Rb1을 합하여 0.8 mg/g 이상 함유하고 있어야 하는데, 백삼을 숙성하였을 때 진세노사이드 Rg1과 Rb1의 합이 기준 100% 이상의 증가율을 보였다. 또한, 숙성 3일에 비해 숙성 7일 진행하는 것이 진세노사이드 3종 및 10종 합이 더 높게 나타났다.The results of comparison of the ginsenoside content before (control) and after ripening of white ginseng main root are shown in Table 2 below. When white ginseng root was aged, ginsenosides were increased overall. The index component content of ginseng should contain more than 0.8 mg/g of ginsenoside Rg1 and Rb1. In addition, the total of 3 and 10 types of ginsenosides was higher in the case of aging for 7 days compared to 3 days of aging.

백삼 주근의 숙성 전후 10종 진세노사이드 함량Content of 10 kinds of ginsenosides before and after ripening of white ginseng main root 진세노사이드 종류Ginsenoside types 대조구control 백삼 주근 숙성 3일차3rd day of ripening of white ginseng main roots 백삼 주근 숙성 7일차7th day of ripening of white ginseng root 함량(mg/g)Content (mg/g) 증가율(%)Increase (%) 함량(mg/g)Content (mg/g) 증가율(%)Increase (%) Rg1Rg1 1.91.9 3.03.0 62.762.7 3.33.3 78.878.8 ReRe 1.11.1 2.72.7 150.1150.1 3.83.8 247.7247.7 RfRf 0.50.5 0.90.9 72.072.0 0.90.9 69.469.4 Rb1Rb1 1.31.3 3.43.4 161.0161.0 3.63.6 172.3172.3 Rg2Rg2 0.10.1 0.20.2 116.0116.0 0.20.2 163.5163.5 RcRc 0.70.7 1.31.3 97.197.1 1.51.5 125.3125.3 Rb2Rb2 0.50.5 0909 82.282.2 0.90.9 79.679.6 Rb3Rb3 0.10.1 0.20.2 75.675.6 0.20.2 107.3107.3 RdRd 0.20.2 0.40.4 160.6160.6 0.60.6 285.6285.6 Rg3Rg3 0.00.0 0.00.0 0.00.0 0.00.0 0.00.0 3종합
(Rg1+Rb1+Rg3)
3 total
(Rg1+Rb1+Rg3)
3.23.2 6.46.4 103.4103.4 6.96.9 117.5117.5
10종합10 total 6.36.3 13.113.1 107.6107.6 15.015.0 138.4138.4

실시예 2. 백삼 세미의 숙성 전후 진세노사이드 함량 및 증가율(%)Example 2. Ginsenoside content and increase rate (%) before and after ripening of white ginseng semi

백삼 세미를 숙성하였을 때 숙성하기 전(대조구)에 비해 백삼근보다 진세노사이드 증가율이 낮았지만, 진세노사이드 함량이 전체적으로 증가하였다. 진세노사이드 종류에서는 Rd가 증가율이 가장 높게 나타났고, 숙성 기간별로는 진세노사이드 3종의 숙성 3일차 및 숙성 7일차 증가율이 각각 43.2% 및 86.7%를 나타내었고, 10종의 숙성 3일차 및 숙성 7일차 증가율은 각각 32.8% 및 81.2%를 나타내어, 숙성 7일차에서 더 높게 나타났다.When white ginseng semi-fermented, the increase rate of ginsenoside was lower than that of white ginseng root compared to before aging (control), but the ginsenoside content was increased overall. In ginsenoside types, Rd showed the highest increase rate, and by aging period, the 3rd and 7th days of aging of the 3 types of ginsenosides showed 43.2% and 86.7%, respectively. The increase rate on the 7th day was 32.8% and 81.2%, respectively, which was higher in the 7th day of aging.

백삼 세미의 숙성 전후 10종 진세노사이드 함량Content of 10 kinds of ginsenosides before and after ripening of white ginseng semi 진세노사이드 종류Ginsenoside types 대조구control 백삼 세미 숙성 3일차White ginseng semi-ripening day 3 백삼 세미 숙성 7일차7th day of semi-ripening of white ginseng 함량(mg/g)Content (mg/g) 증가율(%)Increase (%) 함량(mg/g)Content (mg/g) 증가율(%)Increase (%) Rg1Rg1 2.22.2 3.33.3 49.349.3 3.73.7 68.268.2 ReRe 3.83.8 4.64.6 19.419.4 6.06.0 55.755.7 RfRf 0.80.8 1.21.2 51.951.9 1.41.4 72.672.6 Rb1Rb1 3.43.4 4.74.7 39.239.2 6.86.8 98.698.6 Rg2Rg2 0.30.3 0.40.4 19.819.8 0.60.6 68.068.0 RcRc 2.22.2 2.82.8 27.027.0 4.14.1 86.386.3 Rb2Rb2 1.61.6 1.91.9 21.721.7 2.82.8 77.377.3 Rb3Rb3 0.30.3 0.40.4 28.628.6 0.60.6 98.598.5 RdRd 0.50.5 0.80.8 52.552.5 1.61.6 190.6190.6 Rg3Rg3 0.00.0 0.00.0 0.00.0 0.00.0 0.00.0 3종합
(Rg1+Rb1+Rg3)
3 total
(Rg1+Rb1+Rg3)
5.65.6 8.08.0 43.243.2 10.510.5 86.786.7
10종합10 total 15.215.2 20.220.2 32.832.8 27.627.6 81.281.2

실시예 3. 산삼배양근의 숙성 전후 진세노사이드 함량 및 증가율(%)Example 3. Ginsenoside content and increase rate (%) before and after ripening of wild ginseng cultured root

산삼배양근을 숙성 전과 후 진세노사이드 함량을 비교한 결과는 하기 표 4와 같다. 그 결과, 숙성 7일차에서 Rb1, Rb2 및 Rb3의 함량은 숙성 전에 비해 감소하였고, 진세노사이드 Rg1, Re, Rf, Rg2, Rc, Rd 및 F2 함량은 증가하였다. 특히 F2가 숙성 전에 비해 숙성 7일차 1000% 이상의 증가율을 보였다.The results of comparing the ginsenoside content before and after ripening wild ginseng cultured root are shown in Table 4 below. As a result, on the 7th day of aging, the contents of Rb1, Rb2 and Rb3 were decreased compared to before aging, and the contents of ginsenosides Rg1, Re, Rf, Rg2, Rc, Rd and F2 were increased. In particular, F2 showed an increase rate of more than 1000% on the 7th day of aging compared to before aging.

산삼배양근의 숙성 전후 12종 진세노사이드 함량Content of 12 kinds of ginsenosides before and after ripening of wild ginseng root 진세노사이드 종류Ginsenoside types 대조구control 산삼배양근 숙성 3일차3rd day of ripening of wild ginseng root 산삼배양근 숙성 7일차The 7th day of ripening of wild ginseng root 함량(mg/g)Content (mg/g) 증가율(%)Increase (%) 함량(mg/g)Content (mg/g) 증가율(%)Increase (%) Rg1Rg1 0.50.5 0.30.3 -34.8-34.8 0.70.7 30.530.5 ReRe 1.41.4 1.31.3 -2.7-2.7 1.81.8 28.628.6 RfRf 0.00.0 0.00.0 18.818.8 0.30.3 576.3576.3 Rb1Rb1 2.32.3 1.01.0 -54.6-54.6 1.31.3 -45.5-45.5 Rg2Rg2 0.20.2 0.20.2 -0.3-0.3 0.30.3 77.177.1 RcRc 1.21.2 1.21.2 1.21.2 1.71.7 40.040.0 Rb2Rb2 1.21.2 0.70.7 -39.3-39.3 1.01.0 -19.1-19.1 Rb3Rb3 0.20.2 0.10.1 -36.7-36.7 0.10.1 -22.9-22.9 RdRd 1.61.6 2.02.0 26.626.6 2.92.9 80.780.7 F2F2 0.20.2 1.61.6 642.3642.3 2.42.4 1044.51044.5 Rg3Rg3 0.10.1 0.10.1 7.57.5 0.00.0 -- Rk1Rk1 0.10.1 0.00.0 -- 0.00.0 -- 3종합
(Rg1+Rb1+Rg3)
3 total
(Rg1+Rb1+Rg3)
2.92.9 1.41.4 -49.8-49.8 1.91.9 -33.0-33.0
12종합12 total 8.98.9 8.68.6 -3.5-3.5 12.312.3 37.937.9

실시예 4. 관능평가Example 4. Sensory evaluation

제조예들과 비교예들의 백삼 주근, 백삼 세미 및 산삼배양근의 숙성 전후의 기호도를 비교하기 위해 관능평가를 실시하였으며, 성인 40명을 대상으로 각각의 백삼 및 산삼배양근을 섭취하도록 한 후 향, 맛 및 전반적인 기호도를 아주 나쁠 경우 1점, 아주 좋을 경우 5점으로 하여 평가하게 하였다.A sensory evaluation was conducted to compare the palatability of white ginseng main root, white ginseng semi- and wild ginseng cultured root before and after ripening of the Preparation Examples and Comparative Examples. And overall acceptance was evaluated with 1 point when it was very bad and 5 points when it was very good.

관능평가sensory evaluation 종류Kinds incense taste 전반적인 기호도overall symbolism 백삼 주근white ginseng root 대조구(숙성 전)Control (before aging) 3.73.7 3.03.0 3.23.2 제조예 1Preparation Example 1 4.24.2 4.34.3 4.24.2 비교예 1Comparative Example 1 4.04.0 3.73.7 3.83.8 백삼 세미white ginseng semi 대조구(숙성 전)Control (before aging) 3.63.6 2.92.9 3.03.0 제조예 2Preparation 2 4.34.3 4.54.5 4.34.3 비교예 2Comparative Example 2 3.93.9 3.83.8 3.83.8 산삼배양근wild ginseng cultured root 대조구(숙성 전)Control (before aging) 3.83.8 3.33.3 3.53.5 제조예 3Preparation 3 4.64.6 4.54.5 4.44.4 비교예 3Comparative Example 3 3.93.9 3.93.9 3.93.9

그 결과, 제조예들과 같은 막걸리로 숙성시킨 숙성 백삼 및 숙성 산삼배양근이 가장 높은 점수를 나타내었고, 숙성 전 대조구 시료가 가장 낮은 점수를 나타내었다. 따라서, 제조예들의 조건으로 백삼 및 산삼배양근을 숙성시키는 것이 삼 특유의 쓴맛은 감소되고 부드러운 맛과 향이 증진되어 기호도를 증진시킬 수 있음을 확인할 수 있었다.As a result, aged white ginseng and aged wild ginseng cultured root aged with makgeolli as in Preparation Examples showed the highest score, and the control sample before aging showed the lowest score. Therefore, it was confirmed that aging white ginseng and wild ginseng cultured root under the conditions of the preparations could reduce the characteristic bitter taste of ginseng and enhance the soft taste and aroma, thereby enhancing the preference.

실시예 5. 숙성기간에 따른 숙성 백삼 및 숙성 산삼배양근의 관능평가Example 5. Sensory evaluation of aged white ginseng and aged wild ginseng cultured root according to the aging period

제조예 1, 2, 3의 방법으로 제조하되, (6)단계의 숙성기간을 달리한 제조한 숙성 백삼 및 숙성 산삼배양근을 가지고 실시예 4와 동일한 방법으로 관능평가를 실시하였다.Sensory evaluation was performed in the same manner as in Example 4 using the prepared aged white ginseng and aged wild ginseng cultured root prepared in the method of Preparation Examples 1, 2, and 3, but with different aging periods in step (6).

숙성기간에 따른 숙성 백삼 및 숙성 산삼배양근의 관능평가Sensory evaluation of aged white ginseng and aged wild ginseng cultured root according to the aging period 숙성기간ripening period incense taste 전반적인 기호도overall symbolism 백삼 주근white ginseng root 3일3 days 4.14.1 4.04.0 4.04.0 7일7 days 4.24.2 4.34.3 4.24.2 10일10 days 3.93.9 3.83.8 3.93.9 백삼 세미white ginseng semi 3일3 days 4.14.1 4.14.1 4.14.1 7일7 days 4.34.3 4.54.5 4.34.3 10일10 days 4.04.0 4.04.0 4.04.0 산삼배양근wild ginseng cultured root 3일3 days 4.34.3 4.34.3 4.24.2 7일7 days 4.64.6 4.54.5 4.44.4 10일10 days 4.14.1 4.04.0 4.14.1

숙성기간에 따른 숙성 백삼과 숙성 산삼배양근의 관능평가를 실시한 결과, 모든 숙성삼에서 숙성을 7일 실시하는 것이 가장 높은 점수를 나타내었다. 숙성을 10일 동안 실시할 경우 숙성취가 강하여 약간의 거부감이 느껴져 숙성은 7일 이내로 실시하는 것이 바람직할 것으로 판단된다.As a result of sensory evaluation of aged white ginseng and aged wild ginseng cultured root according to the maturation period, 7 days of aging for all aged ginseng showed the highest score. If the aging is carried out for 10 days, it is judged that it is desirable to carry out the aging within 7 days because the smell of aging is strong and a slight objection is felt.

Claims (6)

삭제delete 삭제delete (1) 감잎, 메밀싹 및 매실 효소액을 2.7~3.3:1.8~2.2:4.5~5.5 중량비율로 혼합한 혼합물을 20~30℃에서 1~3개월 동안 숙성시킨 후 여과하여 여과액을 제조하는 단계;
(2) 쌀 0.8~1.2 kg를 물에 3~5시간 동안 수침한 후 물빼기를 실시하고, 찜통에 넣어 고두밥을 제조하는 단계;
(3) 상기 (2)단계의 제조한 고두밥에 효모 45~55 g을 넣고 20~30℃에서 16~20시간 동안 숙성시키는 단계;
(4) 상기 (3)단계의 숙성시킨 혼합물에 누룩가루 450~550 g, 물 1000~1400 mL 및 상기 (1)단계의 제조한 여과액 0.8~1.2 L를 넣고 혼합한 후 25~35℃에서 5~7일 동안 숙성시키고 여과하여 막걸리를 제조하는 단계;
(5) 백삼 또는 산삼배양근을 상기 (4)단계의 제조한 막걸리에 25~35℃에서 20~28시간 동안 침지하는 단계; 및
(6) 상기 (5)단계의 침지한 백삼 또는 산삼배양근을 꺼낸 후 33~40℃에서 2~8일 동안 숙성시키는 단계를 포함하여 제조하는 것을 특징으로 하는 진세노사이드 함량이 증진된 숙성삼의 제조방법.
(1) A mixture of persimmon leaf, buckwheat sprout and plum enzyme solution in a weight ratio of 2.7~3.3:1.8~2.2:4.5~5.5 is aged at 20~30℃ for 1~3 months and filtered to prepare a filtrate ;
(2) immersing 0.8 to 1.2 kg of rice in water for 3 to 5 hours, then draining the water, and putting it in a steamer to prepare godubap;
(3) adding 45 to 55 g of yeast to the godubap prepared in step (2) and aging at 20 to 30° C. for 16 to 20 hours;
(4) Add 450 to 550 g of yeast powder, 1000 to 1400 mL of water, and 0.8 to 1.2 L of the filtrate prepared in step (1) to the aged mixture in step (3), mix, and then at 25 to 35° C. Aging for 5-7 days and filtering to prepare makgeolli;
(5) immersing white ginseng or wild ginseng cultured root in the makgeolli prepared in step (4) at 25-35° C. for 20-28 hours; and
(6) After taking out the immersed white ginseng or wild ginseng cultured root in step (5), aged ginseng with enhanced ginsenoside content, characterized in that it is prepared including the step of aging at 33-40 ° C. for 2-8 days manufacturing method.
제3항에 있어서,
(1) 감잎, 메밀싹 및 매실 효소액을 2.7~3.3:1.8~2.2:4.5~5.5 중량비율로 혼합한 혼합물을 20~30℃에서 1~3개월 동안 숙성시킨 후 여과하여 여과액을 제조하는 단계;
(2) 쌀 0.8~1.2 kg를 물에 3~5시간 동안 수침한 후 물빼기를 실시하고, 찜통에 넣어 고두밥을 제조하는 단계;
(3) 상기 (2)단계의 제조한 고두밥에 효모 45~55 g을 넣고 20~30℃에서 16~20시간 동안 숙성시키는 단계;
(4) 상기 (3)단계의 숙성시킨 혼합물에 누룩가루 450~550 g, 물 1000~1400 mL 및 상기 (1)단계의 제조한 여과액 0.8~1.2 L를 넣고 혼합한 후 25~35℃에서 5~7일 동안 숙성시키고 여과하여 막걸리를 제조하는 단계;
(5) 백삼을 상기 (4)단계의 제조한 막걸리에 25~35℃에서 20~28시간 동안 침지하는 단계; 및
(6) 상기 (5)단계의 침지한 백삼을 꺼낸 후 33~40℃에서 2~8일 동안 숙성시키는 단계를 포함하여 제조하는 것을 특징으로 하는 Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, Rb3 및 Rd로 이루어진 군으로부터 선택되는 하나 이상의 진세노사이드 함량이 증진된 숙성삼의 제조방법.
4. The method of claim 3,
(1) A mixture of persimmon leaf, buckwheat sprout and plum enzyme solution in a weight ratio of 2.7~3.3:1.8~2.2:4.5~5.5 is aged at 20~30℃ for 1~3 months and filtered to prepare a filtrate ;
(2) immersing 0.8 to 1.2 kg of rice in water for 3 to 5 hours, then draining the water, and putting it in a steamer to prepare godubap;
(3) adding 45 to 55 g of yeast to the godubap prepared in step (2) and aging at 20 to 30° C. for 16 to 20 hours;
(4) Add 450 to 550 g of yeast powder, 1000 to 1400 mL of water, and 0.8 to 1.2 L of the filtrate prepared in step (1) to the aged mixture in step (3), mix, and then at 25 to 35° C. Aging for 5-7 days and filtering to prepare makgeolli;
(5) immersing white ginseng in the makgeolli prepared in step (4) at 25-35° C. for 20-28 hours; and
(6) Rg1, Re, Rf, Rb1, Rg2, Rc, Rb2, characterized in that it is prepared including the step of taking out the immersed white ginseng in step (5) and then aging it at 33-40° C. for 2-8 days. , Rb3 and Rd A method for producing aged ginseng with an enhanced content of one or more ginsenosides selected from the group consisting of.
제3항에 있어서,
(1) 감잎, 메밀싹 및 매실 효소액을 2.7~3.3:1.8~2.2:4.5~5.5 중량비율로 혼합한 혼합물을 20~30℃에서 1~3개월 동안 숙성시킨 후 여과하여 여과액을 제조하는 단계;
(2) 쌀 0.8~1.2 kg를 물에 3~5시간 동안 수침한 후 물빼기를 실시하고, 찜통에 넣어 고두밥을 제조하는 단계;
(3) 상기 (2)단계의 제조한 고두밥에 효모 45~55 g을 넣고 20~30℃에서 16~20시간 동안 숙성시키는 단계;
(4) 상기 (3)단계의 숙성시킨 혼합물에 누룩가루 450~550 g, 물 1000~1400 mL 및 상기 (1)단계의 제조한 여과액 0.8~1.2 L를 넣고 혼합한 후 25~35℃에서 5~7일 동안 숙성시키고 여과하여 막걸리를 제조하는 단계;
(5) 산삼배양근을 상기 (4)단계의 제조한 막걸리에 25~35℃에서 20~28시간 동안 침지하는 단계; 및
(6) 상기 (5)단계의 침지한 산삼배양근을 꺼낸 후 33~40℃에서 6~8일 동안 숙성시키는 단계를 포함하여 제조하는 것을 특징으로 하는 Rg1, Re, Rf, Rg2, Rc, Rd 및 F2로 이루어진 군으로부터 선택되는 하나 이상의 진세노사이드 함량이 증진된 숙성삼의 제조방법.
4. The method of claim 3,
(1) A mixture of persimmon leaf, buckwheat sprout and plum enzyme solution in a weight ratio of 2.7~3.3:1.8~2.2:4.5~5.5 is aged at 20~30℃ for 1~3 months and filtered to prepare a filtrate ;
(2) immersing 0.8 to 1.2 kg of rice in water for 3 to 5 hours, then draining the water, and putting it in a steamer to prepare godubap;
(3) adding 45 to 55 g of yeast to the godubap prepared in step (2) and aging at 20 to 30° C. for 16 to 20 hours;
(4) Add 450 to 550 g of yeast powder, 1000 to 1400 mL of water, and 0.8 to 1.2 L of the filtrate prepared in step (1) to the aged mixture in step (3), mix, and then at 25 to 35° C. Aging for 5-7 days and filtering to prepare makgeolli;
(5) immersing the cultured wild ginseng root in the makgeolli prepared in step (4) at 25-35° C. for 20-28 hours; and
(6) Rg1, Re, Rf, Rg2, Rc, Rd and A method for producing aged ginseng with enhanced content of one or more ginsenosides selected from the group consisting of F2.
제3항 내지 제5항 중 어느 한 항의 방법으로 제조된 진세노사이드 함량이 증진된 숙성삼.Aged ginseng with enhanced ginsenoside content prepared by the method of any one of claims 3 to 5.
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KR20030089649A (en) * 2003-05-28 2003-11-22 주식회사 정문물산 Highly saponin-containing Ginseng steamed red and Producing Method thereof
KR20110050117A (en) * 2009-11-06 2011-05-13 (주)청산녹수 Fermented ginseng materials comprising bio-conversion compound k and manufacturing method, functional rice wine using the same
KR20130099756A (en) * 2012-02-29 2013-09-06 송경호 Fermentated and steamed ginseng and fermentation and steaming method for the same

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030089649A (en) * 2003-05-28 2003-11-22 주식회사 정문물산 Highly saponin-containing Ginseng steamed red and Producing Method thereof
KR20110050117A (en) * 2009-11-06 2011-05-13 (주)청산녹수 Fermented ginseng materials comprising bio-conversion compound k and manufacturing method, functional rice wine using the same
KR20130099756A (en) * 2012-02-29 2013-09-06 송경호 Fermentated and steamed ginseng and fermentation and steaming method for the same

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