KR102256014B1 - Functional health drink for preventing female diseases and process for preparing the same - Google Patents

Functional health drink for preventing female diseases and process for preparing the same Download PDF

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KR102256014B1
KR102256014B1 KR1020190023822A KR20190023822A KR102256014B1 KR 102256014 B1 KR102256014 B1 KR 102256014B1 KR 1020190023822 A KR1020190023822 A KR 1020190023822A KR 20190023822 A KR20190023822 A KR 20190023822A KR 102256014 B1 KR102256014 B1 KR 102256014B1
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weight
parts
tea
herbal medicine
gujeolcho
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KR20200105119A (en
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유창성
김태하
임영제
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우석대학교 산학협력단
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/34Tea substitutes, e.g. matè; Extracts or infusions thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23FCOFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00Tea; Tea substitutes; Preparations thereof
    • A23F3/06Treating tea before extraction; Preparations produced thereby
    • A23F3/14Tea preparations, e.g. using additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D85/00Containers, packaging elements or packages, specially adapted for particular articles or materials
    • B65D85/70Containers, packaging elements or packages, specially adapted for particular articles or materials for materials not otherwise provided for
    • B65D85/804Disposable containers or packages with contents which are mixed, infused or dissolved in situ, i.e. without having been previously removed from the package
    • B65D85/808Disposable containers or packages with contents which are mixed, infused or dissolved in situ, i.e. without having been previously removed from the package for immersion in the liquid to release part or all of their contents, e.g. tea bags
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/30Foods, ingredients or supplements having a functional effect on health
    • A23V2200/302Foods, ingredients or supplements having a functional effect on health having a modulating effect on age
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/20Natural extracts
    • A23V2250/21Plant extracts
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/10Drying, dehydrating
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2300/00Processes
    • A23V2300/14Extraction

Abstract

본 발명은 여성 질병 예방을 위한 기능성 건강 음료 및 그의 제조 방법에 관한 것으로써 좀 더 상세하게는 구절초, 국화, 청보리, 황기를 적절히 처리한 후, 세척, 건조시켜서 제조됨으로써 여성 질병 예방을 위한 기능성을 갖는 차 및 음료에 관한 것이다. 본 발명에 따르면 기능성 차와 음료제품 생산기술의 고도화를 통한 1차 농산물의 고부가가치화 실현할 수 있고, 우수한 지역 농산물의 소비 확대와 6차산업화를 통한 지역경제 활성화에 기여할 수 있다. 또한, 지역내 특화 농산물인 청보리, 황기, 국화, 구절초 등의 생산과 판매 활성화를 통하여 가공기술 고도화, 고용창출 및 다양한 판로 확보로 생산자 및 가공기업의 수익 증가시키는 한편, 특화 작물의 체험관광 자원으로 활용 및 가공제품 생산으로 6차산업화 기반을 마련하는 등 부가적인 이점을 제공할 수 있다.The present invention relates to a functional health drink for preventing female diseases and a method of manufacturing the same, and in more detail, it is prepared by appropriately treating Gujeolcho, chrysanthemum, green barley, and Astragalus followed by washing and drying, thereby providing functionality for preventing female diseases. It relates to having tea and beverages. According to the present invention, it is possible to realize the high value-added of primary agricultural products through the advancement of functional tea and beverage product production technology, and contribute to the activation of the local economy through the expansion of consumption of excellent local agricultural products and the 6th industrialization. In addition, by revitalizing the production and sales of local special agricultural products such as Cheong Barley, Astragalus, Chrysanthemum, Gujeolcho, etc., it increases the profits of producers and processing companies by enhancing processing technology, creating jobs, and securing various sales channels, and as an experiential tourism resource for specialized crops. It can provide additional advantages, such as laying the foundation for 6th industrialization through utilization and production of processed products.

Description

여성 질병 예방을 위한 기능성 건강 음료 및 그의 제조 방법{Functional health drink for preventing female diseases and process for preparing the same}Functional health drink for preventing female diseases and process for preparing the same {Functional health drink for preventing female diseases and process for preparing the same}

본 발명은 여성 질병 예방을 위한 기능성 건강 음료 및 그의 제조 방법에 관한 것으로써 좀 더 상세하게는 구절초, 국화, 청보리, 황기 등을 적절히 처리한 후, 세척 및 건조시켜서 제조됨으로써 여성 질병 예방을 위한 기능성을 갖는 차 및 음료에 관한 것이다.The present invention relates to a functional health drink for preventing female diseases and a method of manufacturing the same, and in more detail, it is prepared by appropriately treating Gujeolcho, chrysanthemum, green barley, Astragalus, etc., followed by washing and drying, and thus functional for preventing female diseases. It relates to having tea and beverages.

2018년 식품 소비시장은 '가성비'의 시대라고 봐도 과언이 아니다. 이러한 추세는 음료시장에도 그대로 적용돼 작년에는 "가격대비 용량" "가격대비 편리함"을 찾는 소비자들에게 맞춤형 음료 제품들이 인기를 끌고 있으며 음료 음용으로 인한 당류 섭취 등 이슈들이 지속적으로 주목받으면서 건강과 프리미엄에 대한 관심도 꾸준히 이어지고 있다. 콜라, 사이다류의 기존 선두 브랜드가 주를 이루는 가운데, 소비자의 건강 추구 니즈에 부합하도록 천연과즙을 혼합하고 저칼로리, 저당 등으로 건강상의 이점을 강조한 탄산음료 제품들도 다양하게 출시되고 있다. 반면 주스 시장은 작년에 이어 시장 성장률이 정체되고 있으며 저과즙 주스와 착즙주스 시장은 성장하고 있으나, 50% 이상의 고함량 주스 제품은 시장이 축소되고 있다. 커피 음료의 경우 최근 가장 가파른 성장률을 보이며 시장이 확대되고 있고, 경기침체의 영향으로 커피에 대해서도 합리적인 소비와 가성비를 중시하는 소비 트렌드가 확대돼 대용량 커피 등 다양한 용량의 RTD 커피가 출시되고 있다. 또한 얼음컵과 함께 구매해 마실 수 있는 파우치커피가 합리적인 가격을 강점으로 큰 성장률을 보이고 있다.It is not an exaggeration to say that the food consumption market in 2018 is the era of'cost performance'. This trend has been applied to the beverage market as it is. Last year, customized beverage products are gaining popularity among consumers looking for "capacity for the price" and "convenience for the price". The interest in is also continuing. Amid the predominantly leading brands of cola and cider, a variety of carbonated beverage products are also being released that blend natural fruit juices to meet consumers' health pursuit needs and emphasize health benefits such as low calorie and low sugar. On the other hand, the juice market has been stagnating since last year, and the low-juice juice and juice markets are growing, but the market for high-content juice products of 50% or more is shrinking. In the case of coffee beverages, the market is expanding with the fastest growth rate in recent years, and as the consumption trend that emphasizes rational consumption and cost-effectiveness for coffee has expanded due to the economic recession, RTD coffee in various capacities such as large-capacity coffee is being released. In addition, pouch coffee that can be purchased and drunk with an ice cup is showing a great growth rate with its strength at a reasonable price.

차 시장은 구입 편리성에 의해 편의점과 대형마트에서 주로 구매가 이루어지는 RTD 차음료 시장이 크게 성장하고 있으며 RTD 차음료 업계는 커피에 사용하는 콜드브루 기법을 차에 응용하거나 고급 찻잎을 우려낸 프리미엄 차 음료를 출시하는 등 색다른 시도를 보이고 있다.In the tea market, the RTD tea beverage market, which is mainly purchased at convenience stores and hypermarkets, is growing significantly due to the convenience of purchase, and the RTD tea beverage industry applies the cold brew technique used for coffee to tea or provides premium tea beverages made from premium tea leaves. It is showing different attempts, such as launching.

2018년 음료 시장은 대형유통업체의 자체브랜드 제품의 출시가 확대되는 등 업계의 위협요소가 증가하고 있는 상황으로 더욱 경쟁상황이 치열해질 것으로 보고 있으며 음료업계는 음료 소재, 패키지 기술 등의 색다른 시도와 변화로 음료 트렌드를 개발, 경쟁력을 강화해 수익을 개선하는 것이 필요하다.In 2018, the beverage market is expected to become more competitive as the industry threats are increasing, such as the expansion of the launch of self-branded products by large retailers. It is necessary to improve profits by developing beverage trends through change and strengthening competitiveness.

중국, 베트남, 인도네시아 등 해외 시장에서의 건강 이슈에 대한 높은 관심에 힘입어 기능성 음료에 대한 업계의 투자가 이어질 것이라는 것이 전문가들의 의견이다. 실제 가장 큰 시장이 될 것으로 전망되는 중국의 연평균 시장 성장률은 15%에 달해 작년에는 약 400억 위안 규모로 커졌으며, 올해는 약 450억 위안 이상을 기록할 것으로 예상하고 있다. Experts say that the industry's investment in functional beverages will continue thanks to high interest in health issues in overseas markets such as China, Vietnam, and Indonesia. In fact, China, which is expected to become the largest market, has an average annual market growth rate of 15%, which has grown to about 40 billion yuan last year, and is expected to reach more than 45 billion yuan this year.

또한 2019 해외 시장의 설탕세 도입 확산이 국내 탄산음료 시장의 성장세에 발목을 잡을 가능성이 높다는 전망도 나오고 있고, 정부와 다수 식음료 업체들도 '당 줄이기'에 동참하고 있어 음료업계의 움직임도 활발해질 것으로 예상된다.In addition, there is a prospect that the spread of the introduction of a sugar tax in the overseas market in 2019 is likely to be hindered by the growth of the domestic carbonated beverage market, and the movement of the beverage industry will also become active as the government and many food and beverage companies are also participating in the'reduction of sugar'. It is expected.

특허청의 특허 출원된 기능성 차의 용도를 살펴보면 암, 고혈압이나 뇌졸증 등 심혈관계 질환, 당뇨 등의 질병 예방을 목적으로 한 것이 60%를 차지하고 있다.Looking at the uses of functional teas patented by the Korean Intellectual Property Office, 60% of them are aimed at preventing diseases such as cancer, cardiovascular diseases such as high blood pressure or stroke, and diabetes.

또한, 유효성분을 함유해 신진대사를 활발하게 하거나 노화를 방지하는 등 건강 증진 목적이 34%, 숙취해소나 피로회복을 목적으로 한 것이 6%를 차지한다.In addition, 34% of them are aimed at promoting health, such as activating metabolism or preventing aging by containing active ingredients, and 6% of those aimed at relieving hangovers or recovering from fatigue.

기능성 차에 관한 특허는 매년 30건 이상 출원되고 있는데 인삼이나 국화, 연잎 등 오래전부터 차의 재료로 사용되고 있던 것부터 동백, 비파, 인동초, 백련, 복분자, 산수유, 두릅, 씀바귀, 민들레 등 매우 다양해졌는데 동의보감 등의 전통 의학서가 참고되고 있다.More than 30 patents on functional tea are filed every year, and from those that have been used for tea since long ago such as ginseng, chrysanthemum, lotus leaf, camellia, loquat, indongcho, white lotus, bokbunja, cornus milk, aralia, scallop, dandelion, etc. Traditional medical books such as et al. are being referenced.

공개특허공보 제10-2017-0095646호(2017년08월23일)Unexamined Patent Publication No. 10-2017-0095646 (August 23, 2017) 등록특허 제10-1564357호(2015년10월23일)Registered Patent No. 10-1564357 (October 23, 2015) 등록번호 제10-1433188호(2014년08월18일)Registration No. 10-1433188 (August 18, 2014)

본 발명자들은 건강음료 제조를 위한 천연물 유래 제품의 기능성과 독성 탐색을 통한 기능성 차, 음료의 레시피 확립과 가공 공정을 개발하기 위하여 예의 연구한 결과 후술하는 바와 같이 한약재를 적절하게 처리한 후 배합하는 등의 처리에 의해 여성들에 대한 기능성이 뛰어난 음료 제품의 제공이 가능한것을 발견하고 본 발명을 완성하기에 이르렀다.As a result of intensive research to develop functional tea and beverage recipes and processing processes by exploring the functionality and toxicity of natural products for the manufacture of healthy beverages, the present inventors blended after appropriately treating herbal medicines as described below. It was discovered that it was possible to provide a beverage product with excellent functionality to women by treatment of, and came to complete the present invention.

따라서, 본 발명의 목적은 건강음료 제조를 위한 천연물 유래 제품의 기능성과 독성 탐색을 통한 기능성 차, 음료의 레시피 확립 건강음료(차, 음료) 생산을 위한 2개 가공 공정을 제공하는 것에 있다.Accordingly, it is an object of the present invention to provide two processing processes for the production of healthy beverages (tea, beverages) to establish a recipe for functional tea and beverage through the search for the functionality and toxicity of natural products for the manufacture of health beverages.

본 발명에 따르면 기능성 차와 음료제품 생산기술의 고도화를 통한 1차 농산물의 고부가가치화 실현할 수 있고, 우수한 지역 농산물의 소비 확대와 6차산업화를 통한 지역경제 활성화에 기여할 수 있다. 또한, 지역내 특화 농산물인 청보리, 황기, 국화, 구절초 등의 생산과 판매 활성화를 통하여 가공기술 고도화, 고용창출 및 다양한 판로 확보로 생산자 및 가공기업의 수익 증가시키는 한편, 특화 작물의 체험관광 자원으로 활용 및 가공제품 생산으로 6차산업화 기반을 마련하는 등 부가적인 이점을 제공할 수 있다.According to the present invention, it is possible to realize the high value-added of primary agricultural products through the advancement of functional tea and beverage product production technology, and contribute to the activation of the local economy through the expansion of consumption of excellent local agricultural products and the 6th industrialization. In addition, by revitalizing the production and sales of local special agricultural products such as Cheong Barley, Astragalus, Chrysanthemum, Gujeolcho, etc., it increases the profits of producers and processing companies by enhancing processing technology, creating jobs, and securing various sales channels, and as an experiential tourism resource for specialized crops. It can provide additional advantages, such as laying the foundation for 6th industrialization through utilization and production of processed products.

도 1은 본 발명에 따른 기능성 차 및 음료의 제조공정도이다.
도 2는 본 발명의 구체적인 실시 형태에 따른 차의 제조 공정도이다.
도 3은 본 발명의 구체적인 실시 형태에 따른 음료의 제조 공정도이다.
도 4는 천연 추출물의 DPPH 라디칼 소거능 시험 결과를 나타내는 그라프도이다.
도 5 내지 7은 세포 생존율을 나타내는 그라프도이다.
도 8은 차의 DPPH 분석 그래프이고, 도 9는 차의 ABTS 분석 그래프도이다.
도 10은 음료의 DPPH 분석 그래프이고, 도 11은 음료의 ABTS 분석 그래프도이다.
도 12 및 13은 차의 MTT Assay 분석 결과이다.
도 14 및 15는 음료의 MTT Assay 분석 결과이다.
1 is a manufacturing process diagram of a functional tea and beverage according to the present invention.
2 is a manufacturing process diagram of a car according to a specific embodiment of the present invention.
3 is a flowchart of a beverage manufacturing process according to a specific embodiment of the present invention.
Figure 4 is a graph showing the DPPH radical scavenging ability test results of natural extracts.
5 to 7 are graphs showing cell viability.
8 is a DPPH analysis graph of tea, and FIG. 9 is an ABTS analysis graph of tea.
10 is a graph of DPPH analysis of beverages, and FIG. 11 is a graph of ABTS analysis of beverages.
12 and 13 are the results of MTT Assay analysis of tea.
14 and 15 are the results of MTT Assay analysis of beverages.

본 발명은, 일면에 있어서, a) 구절초, 황기, 감초, 및 갈근을 포함하는 한약재를 4~6%의 소금물에 1~3 시간 동안 침지시켜 전처리하는 단계;The present invention, in one aspect, a) pre-treatment by immersing the herbal medicines including Gujeolcho, Hwanggi, licorice, and Galgeun in 4-6% salt water for 1 to 3 hours;

b) 상기 한약재로부터 소금물을 버리고 청보리를 추가하여 정제수로 2~3회 세척하는 세척 단계;b) washing step of discarding salt water from the herbal medicine, adding green barley, and washing 2-3 times with purified water;

c) 세척된 한약재를 건조기를 이용하여 60~70℃에서 20~28시간 동안 건조시키는 건조 단계;c) a drying step of drying the washed herbal medicine at 60 to 70° C. for 20 to 28 hours using a dryer;

d) 상기 건조된 한약재를 계량하여 혼합하는 단계; 및d) measuring and mixing the dried herbal medicine; And

e) 혼합된 한약재를 티백으로 포장하는 단계;를 포함하는 것을 특징으로 하는 기능성 차의 제조 방법을 제공한다.e) packaging the mixed herbal medicine with a tea bag; it provides a method for manufacturing a functional tea comprising a.

본 발명은, 추가의 일면에 있어서, 재료의 혼합비는, 전체중량 1.6 중량부를 기준으로, 구절초 0.3 중량부, 황기 0.3 중량부, 감초 0.09 중량부, 갈근 0.3 중량부 및 청보리 0.61 중량부를 포함하는 것을 특징으로 하는 기능성 차의 제조 방법을 제공한다.The present invention, in a further aspect, the mixing ratio of the material, based on the total weight of 1.6 parts by weight, that includes 0.3 parts by weight of Gujeolcho, 0.3 parts by weight of Astragalus, 0.09 parts by weight of licorice, 0.3 parts by weight of brown barley, and 0.61 parts by weight of green barley It provides a method of manufacturing a functional tea characterized by.

본 발명은, 다른 추가의 일면에 있어서,The present invention, in another further aspect,

a) 구절초, 황기, 감초, 및 갈근을 포함하는 한약재를 4~6%의 소금물에 1~3 시간 동안 침지시켜 전처리하는 단계;a) pre-treatment by immersing the herbal medicines including Gujeolcho, Hwanggi, Licorice, and Galgeun in 4-6% salt water for 1-3 hours;

b) 상기 한약재로부터 소금물을 버리고 법제를 하지 않은 청보리를 추가하여 정제수로 2~3회 세척하는 세척 단계;b) a washing step of discarding salt water from the herbal medicine, adding unlegated green barley, and washing 2-3 times with purified water;

c) 세척된 한약재를 건조기를 이용하여 60~70℃에서 20~28시간 동안 건조시키는 건조 단계;c) a drying step of drying the washed herbal medicine at 60 to 70° C. for 20 to 28 hours using a dryer;

d) 상기 건조된 한약재를 분쇄하는 단계;d) pulverizing the dried herbal medicine;

e) 분쇄된 한약재를 계량하여 혼합하는 단계; e) measuring and mixing the pulverized herbal medicine;

f) 분쇄된 한약재를 추출 용매로 추출 및 여과하는 추출 단계;f) an extraction step of extracting and filtering the pulverized herbal medicine with an extraction solvent;

g) 추출된 여액을 계량하여 첨가제를 첨가하여 혼합하는 단계;g) weighing the extracted filtrate, adding an additive, and mixing;

h) 혼합된 여액을 포장하는 단계;를 포함하는 것을 특징으로 하는 기능성 음료의 제조 방법을 제공한다.h) packaging the mixed filtrate; it provides a method for producing a functional beverage comprising a.

본 발명은, 다른 추가의 일면에 있어서,
재료의 혼합비는, 전체중량 60 중량부를 기준으로, 구절초 11.45 중량부, 감초 0.4 중량부, 갈근 3.8 중량부, 황기 11.45 중량부, 청보리 22.9 중량부, 딸기 농축액 6 중량부 및 포도 농축액 4 중량부인 것을 특징으로 하는 기능성 음료의 제조 방법을 제공한다.
The present invention, in another further aspect,
Mixing ratio of the ingredients, based on the total weight of 60 parts by weight, Gujeolcho 11.45 parts by weight, licorice 0.4 parts by weight, Galgeun 3.8 parts by weight, astragalus 11.45 parts by weight, green barley 22.9 parts by weight, strawberry concentrate 6 parts by weight and grape concentrate 4 parts by weight It provides a method for producing a functional beverage characterized by.

삭제delete

이하, 본 발명에 따른 기능성 차 및 음료의 제조 방법에 대하여 첨부 도면을 참조하여 더욱 구체적으로 설명한다.Hereinafter, a method of manufacturing a functional tea and beverage according to the present invention will be described in more detail with reference to the accompanying drawings.

본 명세서 및 청구범위에 사용된 용어나 단어는 통상적이거나 사전적인 의미로 한정 해석되지 아니하며, 발명자는 그 자신의 발명을 가장 최선의 방법으로 설명하기 위해 용어의 개념을 적절하게 정의할 수 있다는 원칙에 입각하여 본 발명의 기술적 사상에 부합하는 의미와 개념으로 해석되어야만 한다. 따라서, 본 명세서에 기재된 실시예와 도면에 도시된 구성은 본 발명의 가장 바람직한 일 실시예에 불과할 뿐이므로, 본 출원 시점에 있어서 이들을 대체할 수 있는 다양한 균등물과 변형예들이 있을 수 있음을 이해하여야 한다.Terms and words used in the present specification and claims are not limited to the usual or dictionary meanings, and the inventor is based on the principle that the concept of terms can be appropriately defined in order to describe his or her invention in the best way. It should be interpreted as a meaning and concept consistent with the technical idea of the present invention. Therefore, it is understood that the embodiments described in the present specification and the configurations shown in the drawings are only the most preferred embodiments of the present invention, and there may be various equivalents and modifications that can replace them at the time of the present application. shall.

도 1은 본 발명에 따른 기능성 차 및 음료의 제조공정도이고, 도 2는 본 발명의 구체적인 실시 형태에 따른 차의 제조 공정도이며, 도 3은 본 발명의 구체적인 실시 형태에 따른 음료의 제조 공정도이다.1 is a manufacturing process diagram of a functional tea and beverage according to the present invention, FIG. 2 is a manufacturing process diagram of tea according to a specific embodiment of the present invention, and FIG. 3 is a manufacturing process diagram of a beverage according to a specific embodiment of the present invention.

도 1 내지 3에 나타낸 바와 같이 본 발명에 따른 기능성 차의 제조 방법은 법제(침지) 단계, 세척 단계, 건조 단계 및 계량 단계로 구분된다.As shown in Figs. 1 to 3, the method of manufacturing a functional tea according to the present invention is divided into a legal preparation (immersion) step, a washing step, a drying step, and a weighing step.

먼저, a) 법제 단계에서는 구절초, 황기, 감초, 및 갈근을 포함하는 한약재를 4~6%의 소금물에 1~3 시간 동안 침지시켜 전처리한다. 이와 같이 법제하는 이유는 한약제를 소독함과 동시에 독성을 완화 또는 해독시키는데 있다.First, a) In the legislative stage, herbal medicines including Gujeolcho, Hwanggi, Licorice, and Galgeun are pretreated by immersing them in 4-6% salt water for 1 to 3 hours. The reason for this legislation is to disinfect or detoxify the toxicity while disinfecting herbal medicines.

그 다음, b) 세척 단계에서는 상기 한약재로부터 소금물을 버리고 법제를 하지 않은 청보리도 추가하여 정제수로 2~3회 세척한다.Then, in the step b) washing, the salt water is discarded from the herbal medicine, and green barley that has not been legally formulated is also added, and washed 2-3 times with purified water.

그 다음, c) 건조 단계에서는 세척된 한약재를 건조기를 이용하여 60~70℃에서 20~28시간 동안 건조시킨다.Then, in the drying step c), the washed herbal medicine is dried for 20 to 28 hours at 60 to 70°C using a dryer.

이어서, d) 계량 및 혼합 단계에서는 상기 한약재를 계량하여 구절초 0.3 중량부, 감초 0.09 중량부, 갈근 0.3 중량부, 칡 0.3 중량부, 및 청보리 0.61 중량부를 포함하도록 혼합한다.Subsequently, d) in the weighing and mixing step, the herbal medicinal material is weighed and mixed to include 0.3 parts by weight of Gujeolcho, 0.09 parts by weight of licorice, 0.3 parts by weight of galgeun, 0.3 parts by weight of arrowroot, and 0.61 parts by weight of green barley.

이 때, 혼합물에 히비스커스 0.1 중량부를 첨가하는 것이 더욱 바람직할 수 있다.At this time, it may be more preferable to add 0.1 parts by weight of hibiscus to the mixture.

그 다음 혼합물을 티백으로 포장하여 기능성 차를 제조한다.Then, the mixture is packaged in a tea bag to make a functional tea.

이와 같은 기능성 차 제품은 뜨거운 물 150 중량부에 넣고, 2분 정도 우린 다음에 음용할 수 있다.These functional tea products can be consumed after adding 150 parts by weight of hot water and simmering for about 2 minutes.

한편, 본 발명에 따른 여성 질병 예방을 위한 기능성 음료의 제조 방법은 도 3에 나타낸 바와 같이, 법제 단계, 세척 단계, 건조 단계, 분쇄 단계, 1차 계량 단계, 추출 단계, 2차 계량 단계, 및 포장 단계로 구분된다.On the other hand, as shown in Figure 3, the method of manufacturing a functional beverage for preventing female diseases according to the present invention is a legal step, a washing step, a drying step, a crushing step, a first weighing step, an extraction step, a second weighing step, and It is divided into packaging stages.

상기 a) 법제 단계, b) 세척 단계, 및 c) 건조 단계는 차의 제조 공정과 동일하다.The a) legal step, b) washing step, and c) drying step are the same as the tea manufacturing process.

이어서, d) 분쇄 단계에서는 건조된 한약재를 분쇄기를 이용하여 적당한(예를 들면, 50~100 메쉬 입도로 분쇄한다.Subsequently, d) in the pulverization step, the dried herbal medicine is pulverized into a suitable (for example, 50-100 mesh particle size) using a pulverizer.

그 다음 e) 계량 단계에서는 분쇄된 한약재를 계량하여 혼합한다.Then, in the e) weighing step, the pulverized herbal medicines are weighed and mixed.

상기 한약재의 혼합비는 한약재 전체 중량 50 중량부를 기준으로, 구절초 11.45 중량부, 감초 0.4 중량부, 갈근 3.8 중량부, 황기 11.45 중량부 및 청보리 22.9 중량부인 것이 가장 바람직할 수 있다.The mixing ratio of the herbal medicine may be most preferably 11.45 parts by weight of Gujeolcho, 0.4 parts by weight of licorice, 3.8 parts by weight of Galgeun, 11.45 parts by weight of Astragalus and 22.9 parts by weight of green barley, based on 50 parts by weight of the total weight of the herbal medicine.

이어서 f) 추출 단계에서는 분쇄된 한약재를 추출 용매로 추출한 후 여과하여 여액을 음료의 원액으로 사용한다.Subsequently, in the extraction step f), the pulverized herbal medicine is extracted with an extraction solvent, filtered, and the filtrate is used as a stock solution for a beverage.

상기 추출은 한약재에 5~15 배수(중량 기준)의 추출 용매를 사용하여 100℃ 전후의 온도에서 90분 내외로 추출하여 수행할 수 있다.The extraction may be performed by extracting for about 90 minutes at a temperature around 100° C. using an extraction solvent of 5 to 15 times (by weight) in the herbal medicine.

상기 추출 용매는 정제수 또는 탄소원자수 4 이하의 저급 알코올 또는 주정을 바람직하게 사용할 수 있다.The extraction solvent may preferably be purified water or a lower alcohol or alcohol having 4 or less carbon atoms.

그 다음, g) 추출된 여액을 계량하여 첨가제를 첨가하여 혼합한다.Then, g) the extracted filtrate is weighed and an additive is added and mixed.

이 때 첨가제로는 꿀, 딸기 농축액, 또는 포도 농축액을 전체 음료 조성을 기준으로 10중량% 이내의 양으로 첨가하여 사용할 수 있다.In this case, as an additive, honey, strawberry concentrate, or grape concentrate may be added and used in an amount within 10% by weight based on the total beverage composition.

마지막으로, h) 혼합된 액상 음료 성분을 스파우트, 파우치, 병 등에 통상의 방법에 의해 포장하여 출하할 때까지 보관한다.Finally, h) the mixed liquid beverage ingredients are packaged in a spout, pouch, bottle, etc. by a conventional method and stored until shipment.

<실시예><Example>

이하, 본 발명은 다음의 대표적인 실시예에 의하여 더욱 구체적으로 설명되나, 본 발명이 이들 실시예에 의해 어떤 식으로든 제한되는 것은 아니다. Hereinafter, the present invention will be described in more detail by the following representative examples, but the present invention is not limited in any way by these examples.

제조예 1: 천연 추출물의 제조Preparation Example 1: Preparation of natural extract

천연물로는 갈근, 황기, 구절초, 청보리, 감초 총 5가지의 한약재를 선정하였다. 그 후 10% 주정 추출물과 100% 물 추출물의 차이점을 확인하고자 각각 한약재 10g과 물 90ml, 한약재 10g과 10% 주정 90ml의 비율로 혼합하였고 열풍건조기를 이용하여 85~100℃, 24시간 조건으로 추출하였다.Five kinds of herbal medicinal herbs were selected as natural products, Galgeun, Hwanggi, Gujeolcho, Cheongbarley, and Licorice. After that, in order to check the difference between 10% alcohol extract and 100% water extract, 10g of herbal medicine and 90ml of water, 10g of herbal medicine and 90ml of 10% alcohol were mixed and extracted under conditions of 85~100℃, 24 hours using a hot air dryer. I did.

시험예 1: 천연 추출물의 이화학적 평가Test Example 1: Physicochemical evaluation of natural extract

추출된 각각의 천연물을 당도, 염도, pH, 색도를 분석하기 위하여 다음과 같은 방법을 이용하였다.The following method was used to analyze the sugar content, salinity, pH, and color of each extracted natural product.

(1) 당도(1) sugar content

당도계(Pocket PAL-1, ATAGO, Japan)를 사용하여 시료의 당도를 측정함. 3회 반복 측정하여 평균값으로 표시함.The sugar content of the sample was measured using a sugar content meter (Pocket PAL-1, ATAGO, Japan). It is measured repeatedly 3 times and displayed as an average value.

(2) 염도(2) salinity

염도계(Pocket PAL-03S, ATAGO, Japan)를 이용하여 3회 반복 측정하여 평균값으로 표시함.It is measured repeatedly 3 times using a salinity meter (Pocket PAL-03S, ATAGO, Japan) and expressed as an average value.

(3) pH(3) pH

pH meter(SARTORIUS, USA)를 사용하여 시료의 pH를 측정함. 각 분석은 3회 반복하여 평균값을 계산함.Measure the pH of the sample using a pH meter (SARTORIUS, USA). Each analysis was repeated 3 times to calculate the average value.

(4) 색도(4) chromaticity

색차계(Minolta CM-3500D)를 사용하여 시료의 색도를 측정함. 3회 반복 측정하여 평균값으로 계산함.The chromaticity of the sample is measured using a color difference meter (Minolta CM-3500D). Measured repeatedly 3 times and calculated as an average value.

이화학 분석을 한 결과를 다음의 표 1, 2에 나타내었다. 갈근 같은 경우는 10% 주정추출물은 8.6 Brix으로 측정되었고 100% 물추출물은 6.1 Brix로 약 1.7 Brix의 차이가 보였고 다른 천연물은 최소 3 Brix에서 최대 3.3 Brix의 차이가 확인되었음. 염도에서는 최소 1%에서 최대 2.53%의 차이가 확인되었다.The results of the physicochemical analysis are shown in Tables 1 and 2 below. In the case of brown root, 10% alcohol extract was measured as 8.6 Brix, and 100% water extract was 6.1 Brix, showing a difference of about 1.7 Brix, and other natural products were found to have a difference of at least 3 Brix and up to 3.3 Brix. In salinity, a difference of at least 1% and at most 2.53% was confirmed.

Figure 112019021190603-pat00001
Figure 112019021190603-pat00001

Figure 112019021190603-pat00002
Figure 112019021190603-pat00002

시험예 2: 천연추출물의 기능성(항산화) 및 세포독성 분석Test Example 2: Analysis of functionality (antioxidation) and cytotoxicity of natural extracts

2-1. DPPH 라디칼 소거능2-1. DPPH radical scavenging activity

96well plate에 시료 40μL + 0.2mM DPPH 160μL 넣고 37℃에서 30분간 빛을 차단 후 반응시키고 ELISA 기기를 이용하여 517nm에서 측정하였으며, Positive control로는 Ascorbic acid를 사용하였다.40 μL of sample + 160 μL of 0.2mM DPPH was put in a 96 well plate, and reacted after blocking light at 37° C. for 30 minutes, and measured at 517 nm using an ELISA device, and ascorbic acid was used as a positive control.

각각의 천연 추출물을 10배 희석하여 DPPH radical scavenging ability 실험을 한 결과를 도 4에 나타내었다. 도 4는 천연 추출물의 DPPH 라디칼 소거능 시험 결과를 나타내는 그라프도이다. 그 결과 청보리는 10%주정 추출물이 24.69mg/L, 100% 물 추출물이 8.59mg/L으로 약 16.1mg/L의 차이가 보인 방면 다른 천연물들은 큰 차이를 보이지 않는 것을 확인하였다. 황기 외 4가지 소재는 항산화 활성을 나타냈다.Figure 4 shows the results of the DPPH radical scavenging ability experiment by diluting each of the natural extracts 10 times. Figure 4 is a graph showing the DPPH radical scavenging ability test results of natural extracts. As a result, it was confirmed that the difference of about 16.1mg/L in green barley was 24.69mg/L in 10% alcohol extract and 8.59mg/L in 100% water extract, whereas other natural products did not show much difference. Four other materials, including Hwanggi, showed antioxidant activity.

2-2. MTT Assay2-2. MTT Assay

자궁암 세포주 (HeLa)는 ㈜ 한국세포주은행에서 분양받아 -196℃ 액체질소 탱크에 보관하여 사용하였다. 자궁암 세포주인 HeLa 세포를 96well plate에 3x104 cells/well이 되도록 세포수를 조정한 다음, 시료를 1~40μg/ml의 농도로 처리하여 배양기(37℃의 5% CO2, 24시간) 배양하였으며 배양종료 4시간 전에 5mg/ml 농도로 PBS(pH 7.4)에 희석된 MTT용액 20μl를 각 well에 처리하고, 0.1N HCL에 녹인 10% SDS 100μl로 용해시켜 18시간 동안 은박지로 빛을 차단하였다. 발색된 각 well의 흡광도를 ELISA reader를 이용해서 570nm에서 측정하고 대조군의 흡광도와 비교하여 세포 생존율을 백분율로 환산하였다.The cervical cancer cell line (HeLa) was purchased from Korea Cell Line Bank, Inc. and stored in a liquid nitrogen tank at -196°C. HeLa cells, a cervical cancer cell line, were adjusted to 3×10 4 cells/well in a 96 well plate, and then the samples were treated at a concentration of 1 to 40 μg/ml and cultured in an incubator (37° C. 5% CO 2 , 24 hours). 4 hours before the end of the culture, 20 μl of MTT solution diluted in PBS (pH 7.4) at a concentration of 5 mg/ml was treated in each well, dissolved in 100 μl of 10% SDS dissolved in 0.1N HCL, and light was blocked with silver paper for 18 hours. The absorbance of each well developed was measured at 570 nm using an ELISA reader, and the cell viability was converted into a percentage by comparing the absorbance of the control group.

그 결과를 도 5 내지 7에 나타내었다. 도 5 내지 7은 세포 생존율을 나타내는 그라프도이다. 그 결과 청보리와 황기 모두 100% 물로 추출한 시료가 유의적으로 세포생존율을 감소시키고 있음을 확인하였으며, 또 갈근,구절초,감초는 10% 주정으로 추출한 시료가 유의적으로 세포생존율을 감소시키고 있음을 확인하였다. 이러한 결과는 100% 물추출 청보리, 황기, 10% 주정 추출 갈근, 구절초, 감초 추출물이 자궁암세포의 증식을 억제시켜 항암활성을 나타냄을 의미한다.The results are shown in FIGS. 5 to 7. 5 to 7 are graphs showing cell viability. As a result, it was confirmed that the samples extracted with 100% water for both green barley and Astragalus medicinal herb significantly decreased the cell viability, and the samples extracted with 10% alcohol for Galgeun, Gujeolcho, and licorice were significantly reduced the cell viability. I did. These results indicate that 100% water-extracted green barley, astragalus, 10% alcohol-extracted Galgeun, Gujeolcho, and licorice extracts inhibit the proliferation of uterine cancer cells and show anticancer activity.

실시예 1: 천연물을 이용한 차, 음료의 제조Example 1: Preparation of tea and beverages using natural products

도 1 내지 3에 나타낸 바와 같이, 5% 소금물에 청보리를 제외한 천연물(구절초, 감초, 갈근, 황기)을 넣고 2시간 동안 법제시킨 후 5% 소금물을 버리고 흐르는 물에 2~3번 세척 후 열풍건조기(65℃ 24hr)를 이용하여 건조시킨 다음 시중에서 판매하는 티백의 무게 1.5g 기준으로 1:1:1:1:1:으로 제조하였다. As shown in Figs. 1 to 3, natural products (gujeolcho, licorice, galgeun, astragalus) excluding green barley were added to 5% salted water and legalized for 2 hours, then discarded 5% salt water and washed 2-3 times in running water, followed by a hot air dryer. After drying using (65° C. 24hr), it was prepared as 1:1:1:1:1: based on the weight of 1.5g of a commercially available tea bag.

표 3은 차에 관한 함량표이고, 표 4는 음료에 관한 함량표이다. 표 3 내지 7에 나타낸 바와 같이, 관능평가 결과 감초의 단맛이 너무 강하였고, 청보리의 고소한 맛을 높이기 위하여 2차에서는 감초의 함량을 줄이고 청보리의 함량을 높여 제조하였다.Table 3 is a content table for tea, and Table 4 is a content table for beverages. As shown in Tables 3 to 7, as a result of sensory evaluation, the sweetness of licorice was too strong, and in order to enhance the savory taste of green barley, the content of licorice was reduced and the content of green barley was increased in the second stage.

2차 결과 감초의 단맛이 1차보다는 약해졌으나 아직도 강하여 0.1g에서 0.09g으로 줄이고 대신 청보리의 함량을 추가하여 제조하였다. 3차 레시피에 색과 향을 증진시키기 위해 건조된 히비스커스 0.2g를 넣었으나 신맛이 너무 강하여 최종적으로 0.1g으로 줄여서 제조하였다.As a result of the second, the sweet taste of licorice was weaker than that of the first, but it was still strong, and it was reduced from 0.1g to 0.09g, and instead, the content of green barley was added. In the third recipe, 0.2 g of dried hibiscus was added to enhance color and aroma, but the sour taste was so strong that it was finally reduced to 0.1 g.

Figure 112019021190603-pat00003
Figure 112019021190603-pat00003

Figure 112019021190603-pat00004
Figure 112019021190603-pat00004

시중에서 판매되는 제품을 구입하여 실시예에서 제조된 차와 음료의 이화학 분석, 항산화, 세포독성, 아미노산 분석의 비교분석을 하고자 아래의 표 5과 같이 각각의 시료의 명칭을 임의로 정하였다.In order to conduct a comparative analysis of the physicochemical analysis, antioxidant, cytotoxicity, and amino acid analysis of the tea and beverage prepared in the examples by purchasing commercially available products, the names of each sample were arbitrarily determined as shown in Table 5 below.

차 시료명Tea sample name 주요 내용Main Content 음료 시료명Beverage sample name 주요 내용Main Content TP-1TP-1 A사 시판품Commercially available product of Company A DP-1DP-1 H사 시판품Commercially available product of company H TP-2TP-2 G사 시판품G company commercial product DTP-1DTP-1 2차 시제품(표 4 참조)Second prototype (see Table 4) TP-3TP-3 D사 시판품Commercial product of D company DTP-2DTP-2 시제품(표 4 참조)Prototype (see Table 4) TTP-1TTP-1 3차 시제품(표 3 참조)Third prototype (see Table 3) DTP-3DTP-3 시제품(표 4 참조)Prototype (see Table 4) TTP-2TTP-2 2차 시제품(표 3 참조)Second prototype (see Table 3) DTP-4DTP-4 3차 시제품(표 4 참조)Third prototype (see Table 4) TP(Tea Product), TTP(Tea Test Product)TP(Tea Product), TTP(Tea Test Product) TP(Drink Product), TTP(Drink Test Product)TP(Drink Product), TTP(Drink Test Product)

시험예 3: 차, 음료의 이화학적 평가Test Example 3: Physicochemical evaluation of tea and beverage

3-1: 대조군과 시료군(차)의 당도, 염도, pH, 색도 평가3-1: Evaluation of sugar content, salinity, pH, and chromaticity of the control group and the sample group (tea)

시료와 대조군의 차 및 음료에 대하여 이화학적 분석을 수행하고 그 결과를 표 6에 나타내었다. 표 6는 차에 대한 결과이고, 표 7은 음료에 대한 결과이다.Physicochemical analysis was performed on the tea and beverages of the sample and the control group, and the results are shown in Table 6. Table 6 is the results for tea, and Table 7 is the results for beverages.

Figure 112019021190603-pat00005
Figure 112019021190603-pat00005

차 시판품과 시제품의 이화학성 분석 결과 당도, 염도에서는 큰 차이가 없었으나, pH 에서는 TTP- 2가 다른 시료들에 비해 4.48로 낮게 측정됨 차의 향을 향상시키기 위해 첨가된 히비스커스의 영향으로 보임. 색도에서는 L값(명도), a값(적색도)은 큰 차이가 없었으나, b값(황색도)에서는 TP 1 89.33로 가장 높게 측정되었고 반대로 TTP-2가 9.25로 가장 낮은 값을 나타냈다.As a result of the physicochemical analysis of the tea commercial product and the prototype product, there was no significant difference in sugar content and salinity, but in pH, TTP-2 was measured to be 4.48 lower than that of other samples. It seems to be the effect of hibiscus added to improve the flavor of tea. In chromaticity, there was no significant difference in L value (brightness) and a value (redness), but in b value (yellowness), TP 1 was measured the highest as 89.33, and on the contrary, TTP-2 was the lowest as 9.25.

Figure 112019021190603-pat00006
Figure 112019021190603-pat00006

음료 시판품과 시제품의 이화학성 분석 결과 DTP-4가 당도 11.1 Brix, 염도9.3%, pH3.77로 다른 시료군에 비해 가장 높게 측정되었고, 색도에서는 DP-1(L값 71.63, a값 11.10, b값 49.31) DTP-4(L값 40.35, a값 30.32, b값 54.80)를 제외한 다른 시료군은 L값(명도), a값(적색도), b값(황색도)에서 큰 차이가 나타나지 않았다.As a result of physicochemical analysis of commercial beverages and prototypes, DTP-4 was measured to be the highest with a sugar content of 11.1 Brix, a salinity of 9.3%, and a pH of 3.77 compared to other sample groups, and in chromaticity, DP-1 (L value 71.63, a value 11.10, b) Value 49.31) Except for DTP-4 (L value 40.35, a value 30.32, b value 54.80), there was no significant difference in L value (brightness), a value (redness), and b value (yellowness). .

시험예 4: 차 및 음료의 기능성(항산화) 및 세포독성 분석 Test Example 4: Functional (antioxidant) and cytotoxicity analysis of tea and beverages

4-1: 차 및 음료의 기능성(항산화) 분석4-1: Functional (antioxidant) analysis of tea and beverages

실시예 1의 차 및 음료의 항산화 기능성을 분석하고 그 결과를 도 8 내지 11에 나타내었다.The antioxidant functionality of the tea and beverage of Example 1 was analyzed, and the results are shown in FIGS. 8 to 11.

ABTS 라디칼 소거능은 다음과 같이 수행하였다. 7.4 mM의 ABTS와 2.6 mM potassium persulfate를 혼합하여 실온·암소에서 24시간 동안 방치하여 radical을 형성시킨다. ABTS 용액은 실험 직전에 732nm에서 흡광도가 0.700±0.030(mean±SE)이 되도록 phosphate-buffered saline(pH7.4)으로 희석하여 사용하였다. 농도별 추출물 50 μL에 ABTS 용액 950 μL를 첨가하여 암소에서 10분간 반응시킨 후 732nm에서 흡광도를 측정하였다. Positive control로 Ascorbic acid 사용하였다.ABTS radical scavenging activity was performed as follows. 7.4 mM ABTS and 2.6 mM potassium persulfate are mixed and left at room temperature for 24 hours to form radicals. The ABTS solution was diluted with phosphate-buffered saline (pH 7.4) so that the absorbance at 732 nm was 0.700±0.030 (mean±SE) immediately before the experiment. 950 μL of the ABTS solution was added to 50 μL of the extract for each concentration, reacted in the dark for 10 minutes, and the absorbance was measured at 732 nm. Ascorbic acid was used as a positive control.

도 8은 차의 DPPH 분석 그래프이고, 도 9는 차의 ABTS 분석 그래프도이다. 청보리, 황기, 감초, 구절초, 갈근을 이용한 차의 항산화 활성을 측정하였다. 전체적으로 ABTS 레디컬 소거능이 DPPH 레디컬 소거능보다 높게 나타났다.8 is a DPPH analysis graph of tea, and FIG. 9 is an ABTS analysis graph of tea. The antioxidant activity of tea using green barley, astragalus, licorice, gujeolcho, and galgeun was measured. Overall, ABTS radical scavenging ability was higher than DPPH radical scavenging ability.

열수로 추출한 시료의 특성상 물, 소수성 용매 양쪽에 용해되는 ABTS가 높게 측정이 되며, 에탄올에 용해되어야 항산화능력이 측정이 되는 DPPH가 좀더 낮게 측정된 것으로 추정된다. ABTS 레디컬 소거능의 경우 전체적인 산화 환원능력은 TP3이 다른 시료의 70% 수준에 머무르지만 DPPH에서는 TP1이 86.73mg/L으로 가장 높으며 TTP2 44.24mg/L, TP3 38.65mg/L, TP2 25.56mg/L, TTP1 23.44mg/L 순으로 측정되었다.Due to the characteristics of the sample extracted with hot water, the ABTS dissolved in both water and hydrophobic solvents was measured high, and the DPPH, which was dissolved in ethanol to measure the antioxidant capacity, was estimated to be measured lower. In the case of ABTS radical scavenging activity, the overall redox capacity of TP3 remains at the level of 70% of other samples, but in DPPH, TP1 is the highest at 86.73mg/L, TTP2 44.24mg/L, TP3 38.65mg/L, and TP2 25.56mg/L L, TTP1 was measured in the order of 23.44mg/L.

도 10은 음료의 DPPH 분석 그래프이고, 도 11은 음료의 ABTS 분석 그래프도이다.10 is a graph of DPPH analysis of beverages, and FIG. 11 is a graph of ABTS analysis of beverages.

청보리, 황기, 감초, 구절초 및 갈근의 추출물로 제조한 음료의 항산화 활성을 측정하였다. ABTS 레디컬 소거능과 DPPH 레디컬 소거능의 차이가 DP-1, DTP-1~3까지는 크게 나타나지 않으며, DTP-4의 경우 ABTS 레디컬 소거능이 DPPH 레디컬 소거능 보다 약 2.5배 높게 측정되었다.The antioxidant activity of beverages prepared with extracts of Cheongbarley, Astragalus, Licorice, Gujeolcho and Galgeun was measured. The difference between the ABTS radical scavenging ability and DPPH radical scavenging ability did not appear significantly until DP-1, DTP-1 to 3, and in the case of DTP-4, the ABTS radical scavenging ability was measured to be approximately 2.5 times higher than the DPPH radical scavenging ability.

4-2) 차 및 음료의 MTT 분석4-2) MTT analysis of tea and beverages

청보리, 황기, 감초, 구절초, 갈근을 이용한 차의 항암 활성에 미치는 영향을 알아보기 위하여 HeLa세포에 농도별(1~100μg/ml)로 처리하여 24시간 동안 처리한 후 세포생존율을 측정한 결과를 도 12 및 13에 나타내었다. 도 12 및 13은 차의 MTT Assay 분석 결과이다. 도 12 및 13에 나타낸 바와 같이, 차1, 차2, 차3, 차4, 차5 시료 모두 유의적으로 세포생존율을 감소시키고 있음을 확인하였다. 또 이러한 결과는 구절초, 감초, 칡을 첨가한 추출물을 이용한 차가 자궁암세포의 증식을 억제시켜 항암활성을 나타내는 것으로 확인되었다. In order to investigate the effect on the anticancer activity of tea using green barley, astragalus, licorice, gujeolcho, and brown root, HeLa cells were treated at different concentrations (1~100μg/ml), treated for 24 hours, and then the cell viability was measured. It is shown in Figures 12 and 13. 12 and 13 are the results of MTT Assay analysis of tea. As shown in Figs. 12 and 13, it was confirmed that the cell viability was significantly reduced in all samples of tea 1, tea 2, tea 3, tea 4, and tea 5. In addition, these results were confirmed that tea using extracts containing Gujeolcho, licorice, and arrowroot showed anticancer activity by inhibiting the proliferation of uterine cancer cells.

한방추출물을 이용한 음료의 항암 활성에 미치는 영향을 알아보기 위하여 HeLa세포에 농도별(1~100μg/ml)로 처리하여 24시간 동안 처리한 후 세포생존율을 측정한 결과를 도14 및 15에 나타내었다.In order to investigate the effect on the anticancer activity of beverages using herbal extracts, HeLa cells were treated at different concentrations (1-100 μg/ml) and treated for 24 hours, and then the results of measuring cell viability are shown in Figs. 14 and 15. .

도 14 및 15는 음료의 MTT Assay 분석 결과이다. 음료1, 음료4, 음료5를 첨가한 시료가 유의적으로 세포생존율을 감소시키고 있음을 확인하였다. 또 음료2, 음료 3을 첨가한 시료는 유의적으로 세포생존율을 감소시키고 있음을 확인하였다. 이러한 결과는 음료 4가 자궁암세포의 증식을 억제시켜 항암활성을 나타내는 것으로 확인되었다.14 and 15 are the results of MTT Assay analysis of beverages. It was confirmed that the samples added with Beverage 1, Beverage 4, and Beverage 5 significantly reduced the cell viability. In addition, it was confirmed that the samples to which beverage 2 and beverage 3 were added significantly reduced the cell viability. These results confirmed that beverage 4 exhibits anticancer activity by inhibiting the proliferation of uterine cancer cells.

시험예 5: 차 및 음료의 아미노산 및 기능성 아미노산 분석Test Example 5: Analysis of amino acids and functional amino acids in tea and beverages

아미노산 추출은 EP Tube에 800㎕의 용매(methanol: chloroform: water=12:5:3)를 시료 200mg에 가하여 혼합 사용하고 혼합액을 원심분리하여 수용액 층인 상층액을 1차 회수하고, 유기용매층에 클로로포름과 물(1:2) 혼합액 600 ㎕을 가하여 혼합한 후 원심분리하여 상층액을 2차 회수함. 1, 2차 회수한 상층액을 합하여 동결건조 하고, 초순수로 용해하여 여과 후 HPLC 분석용 시료로 사용하였다.For amino acid extraction, add 800 µl of solvent (methanol: chloroform: water=12:5:3) to the sample 200mg to the EP Tube, mix, and centrifuge the mixed solution to collect the supernatant, which is an aqueous solution layer, first, and then to the organic solvent layer. After adding 600 µl of a mixture of chloroform and water (1:2) and mixing, centrifugation to collect the supernatant for a second time. The first and second collected supernatant were combined, lyophilized, dissolved in ultrapure water, filtered, and used as a sample for HPLC analysis.

HPLC를 이용한 기능성 성분 정량분석은 HPLC(Waters, USA) 분석을 위해 시료는 6-aminoquioly-N-hydroxy succin imidyl carbonate (AQC)로 유도체화하고, 3.9ㅧ150mm AccQㅇTag™(Nova-Pak™ㅵC18, Waters) 칼럼으로 유도체들을 분리하였다. 아미노산 함량은 표준 아미노산의 HPLC 분석결과를 토대로 작성한 표준곡선을 이용하여 산출하였다.For quantitative analysis of functional components using HPLC, samples were derivatized with 6-aminoquioly-N-hydroxy succin imidyl carbonate (AQC) for HPLC (Waters, USA) analysis, and 3.9ㅧ150mm AccQㅇTag™ (Nova-Pak™) C18, Waters) column to separate the derivatives. The amino acid content was calculated using a standard curve prepared based on the results of HPLC analysis of standard amino acids.

시제품 차 및 음료의 총 아미노산 함량 분석 결과 차 보다는 음료에 아미노산 함량이 2~3배 정도의 높은 함량을 가지고 있는 것으로 확인되었다. 기능성 아미노산 GABA, Ori(ornithine) 함량 분석결과 Ori(ornithine)은 전체 시료에서 함유되어 있지 않았으며, 음료 시제품 TPP-1에서 18.83mg/100g으로 가장 높았고 모든 시료에서 GABA 함량이 0 ~ 18.83mg/100g의 함량이 분석되었음. 기능성 아미노산의 함량을 높이기 위해서는 미생물 처리, 발효 등 공정이 추가 개발되어야 할 것으로 판단된다. As a result of analyzing the total amino acid content of the prototype teas and beverages, it was confirmed that the amino acid content in beverages was 2 to 3 times higher than that of tea. As a result of analyzing the content of functional amino acids GABA and Ori (ornithine), Ori (ornithine) was not contained in all samples, and the highest in the beverage prototype TPP-1 at 18.83mg/100g, and GABA content in all samples was 0 ~ 18.83mg/100g The content of was analyzed. In order to increase the content of functional amino acids, it is believed that additional processes such as microbial treatment and fermentation should be developed.

Figure 112019021190603-pat00007
Figure 112019021190603-pat00007

시험예 6: 차, 음료 시제품의 관능평가Test Example 6: Sensory evaluation of tea and beverage prototypes

관능평가 패널 13명을 대상으로 시제품(차 2종류, 음료 4종류)과 시판 제품(차 3종류, 음료 1종류)의 5개 항목 5점 척도의 관능평가를 3회 실시하여 평균값을 구한 결과를 다음의 표 9 및 10에 나타내었다. 표 9는 차 시제품 관능검사 결과이고, 표 10은 음료 시제품 관능검사 결과이다.Sensory evaluation was conducted three times on a five-point scale of 5 items, including prototypes (2 types of tea, 4 types of beverages) and commercial products (3 types of tea, 1 type of beverage), targeting 13 sensory evaluation panels, and the average value was calculated. It is shown in Tables 9 and 10 below. Table 9 is the sensory test result of the tea prototype, and Table 10 is the sensory test result of the beverage prototype.

 구분division TP-1TP-1 TP-2TP-2 TP-3TP-3 TTP-1(3차)TTP-1 (3rd) TTP-2TTP-2 짠맛 Salty 3.63.6 3.63.6 3.43.4 3.63.6 3.53.5 단맛sweetness 3.43.4 3.43.4 3.93.9 3.93.9 3.43.4 신맛Sour taste 2.42.4 2.52.5 2.62.6 2.22.2 2.52.5 incense 3.63.6 3.43.4 3.13.1 3.33.3 3.63.6 선호도preference 22 55 44 1One 33

 구분division DP-1DP-1 DTP-1DTP-1 DTP-2DTP-2 DTP-3DTP-3 DTP-4(3차)DTP-4 (3rd) 짠맛 Salty 3.33.3 3.43.4 3.43.4 3.63.6 3.73.7 단맛sweetness 3.23.2 3.53.5 3.73.7 3.33.3 3.93.9 신맛Sour taste 2.92.9 2.52.5 3.23.2 2.52.5 2.32.3 incense 3.63.6 3.63.6 2.92.9 3.23.2 3.73.7 선호도preference 55 44 33 22 1One

시료 간 점수 편차는 적은 편이었으며, 선호도 결과에서는 차의 경우에는 시제품 TTP-1 3차, 음료의 경우에는 시제품 DTP-4 3차의 선호도가 가장 높은 것으로 나타냈다. The score difference between samples was small, and in the preference result, it was found that the preference of the prototype TTP-1 tertiary in the case of tea and the trial product DTP-4 tertiary in the case of beverages was the highest.

차 및 음료 시장은 국내시장과 세계시장 모두 꾸준하게 성장할 것으로 예상하고 있으며 당 함량이 줄어든 제품의 시장 규모가 점차 확대될 전망이다.The tea and beverage market is expected to grow steadily in both the domestic and global markets, and the market size of products with reduced sugar content is expected to gradually expand.

세계 시장에서 글로벌 기업과의 경쟁에서 우위를 선점하기 위해서는 고유의 기술 확보가 중요하여 이를 이용한 품질 향상과 차 및 음료 수출에 기여할 수 있는 다양한 음료 제품의 발굴과 국제 규격화가 필요하다.In order to dominate the competition with global companies in the global market, it is important to secure unique technologies, so it is necessary to discover various beverage products that can contribute to the improvement of quality and export of tea and beverages and international standardization using them.

식품 생산에서 원료의 선택은 매우 중요한 역할을 하며, 현재 기능성의 천연물을 이용한 식품가공 및 제품 개발이 이루어지고 있다.In food production, the selection of raw materials plays a very important role, and food processing and product development using functional natural products are currently being made.

본 발명의 적용산업 분야는 6차산업 식품분야로 ① 천연물의 고도화를 통한 고부가가치화 ② 6차산업 식품생명 활성화를 통한 지역경제 활성화에 기여. 즉, 우리농산물 이용 확산으로 1차산업 식품 활성화, 우리 농산물 가공 기술 고도화로 2차산업 식품 활성화, 다양화와 마케팅 촉진을 통한 3차산업 식품 활성화를 통하여 6차산업 식품생명 분야를 활성화할 수 있어 LINC+사업단 특화 분야와의 적합성이 매우 높다. 또한, 이를 통해 기업 매출 증대, 청년 일자리 창출, 지역경제 활성화의 선순환 구조를 이루어 산학협력 고도화에 기여하고자 한다.The applied industry of the present invention is the 6th industrial food sector: ① High value-added through the advancement of natural products ② Contributing to vitalization of the local economy by revitalizing the food life of the 6th industry. In other words, it is possible to revitalize the food life sector of the 6th industry through revitalization of foods in the primary industry by spreading the use of Korean agricultural products, revitalization of foods in the secondary industry through the advancement of Korean agricultural products processing technology, and revitalization of foods in the tertiary industry through diversification and marketing promotion. It is highly suited to the LINC+ project group's specialized fields. In addition, through this, we intend to contribute to the advancement of industry-university cooperation by creating a virtuous cycle structure of increasing corporate sales, creating jobs for young people, and revitalizing the local economy.

이상 본 발명은 바람직한 실시예를 참조하여 설명하였지만, 해당 기술 분야의 숙련된 당업자는 하기의 특허 청구의 범위에 기재된 본 발명의 사상 및 영역으로부터 벗어나지 않는 범위 내에서 본 발명을 다양하게 수정 및 변경시킬 수 있음을 이해할 수 있을 것이다. The present invention has been described above with reference to preferred embodiments, but those skilled in the art will be able to variously modify and change the present invention without departing from the spirit and scope of the present invention described in the following claims. You will understand that you can.

Claims (4)

a) 구절초, 황기, 감초 및 갈근을 포함하는 한약재를 4~6%의 소금물에 1~3 시간 동안 침지시켜 전처리하는 단계;
b) 상기 한약재로부터 소금물을 버리고 청보리를 추가하여 정제수로 2~3회 세척하는 세척 단계;
c) 세척된 한약재를 건조기를 이용하여 60~70℃에서 20~28시간 동안 건조시키는 건조 단계;
d) 상기 건조된 한약재를 계량하여 혼합하는 단계; 및
e) 혼합된 한약재를 티백으로 포장하는 단계;를 포함하고, 재료의 혼합비는, 전체중량 1.6 중량부를 기준으로, 구절초 0.3 중량부, 황기 0.3 중량부, 감초 0.09 중량부, 갈근 0.3 중량부 및 청보리 0.61 중량부를 포함하는 것을 특징으로 하는 기능성 차의 제조 방법.
a) pre-treatment by immersing the herbal medicines including Gujeolcho, Hwanggi, licorice and Galgeun in 4-6% salt water for 1 to 3 hours;
b) washing step of discarding salt water from the herbal medicine, adding green barley, and washing 2-3 times with purified water;
c) a drying step of drying the washed herbal medicine at 60 to 70° C. for 20 to 28 hours using a dryer;
d) measuring and mixing the dried herbal medicine; And
e) packaging the mixed medicinal herbs in a tea bag; including, and the mixing ratio of the ingredients is 0.3 parts by weight of Gujeolcho, 0.3 parts by weight of Astragalus, 0.09 parts by weight of licorice, 0.3 parts by weight of galgeun and green barley based on 1.6 parts by weight of the total weight. A method for producing a functional tea, comprising 0.61 parts by weight.
삭제delete a) 구절초, 황기, 감초 및 갈근을 포함하는 한약재를 4~6%의 소금물에 1~3 시간 동안 침지시켜 전처리하는 단계;
b) 상기 한약재로부터 소금물을 버리고 청보리를 추가하여 정제수로 2~3회 세척하는 세척 단계;
c) 세척된 한약재를 건조기를 이용하여 60~70℃에서 20~28시간 동안 건조시키는 건조 단계;
d) 상기 건조된 한약재를 분쇄하는 단계;
e) 분쇄된 한약재를 계량하여 혼합하는 단계;
f) 분쇄된 한약재를 추출 용매로 추출 및 여과하는 추출 단계;
g) 추출된 여액을 계량하여 첨가제를 첨가하여 혼합하는 단계;
h) 혼합된 여액을 포장하는 단계;를 포함하되,
재료의 혼합비는, 전체중량 60 중량부를 기준으로, 구절초 11.45 중량부, 감초 0.4 중량부, 갈근 3.8 중량부, 황기 11.45 중량부, 청보리 22.9 중량부, 딸기 농축액 6 중량부 및 포도 농축액 4 중량부인 것을 특징으로 하는 기능성 음료의 제조 방법.
a) pre-treatment by immersing the herbal medicines including Gujeolcho, Hwanggi, licorice and Galgeun in 4-6% salt water for 1 to 3 hours;
b) washing step of discarding salt water from the herbal medicine, adding green barley, and washing 2-3 times with purified water;
c) a drying step of drying the washed herbal medicine at 60 to 70° C. for 20 to 28 hours using a dryer;
d) pulverizing the dried herbal medicine;
e) measuring and mixing the pulverized herbal medicine;
f) an extraction step of extracting and filtering the pulverized herbal medicine with an extraction solvent;
g) weighing the extracted filtrate, adding an additive, and mixing;
h) packing the mixed filtrate; including,
Mixing ratio of the ingredients, based on the total weight of 60 parts by weight, Gujeolcho 11.45 parts by weight, licorice 0.4 parts by weight, Galgeun 3.8 parts by weight, astragalus 11.45 parts by weight, green barley 22.9 parts by weight, strawberry concentrate 6 parts by weight and grape concentrate 4 parts by weight A method for producing a functional beverage, characterized in that.
삭제delete
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