KR102249976B1 - An Integrated Module of Non-contact type Optical Sensors - Google Patents

Abstract

The non-contact optical sensor integrated module according to the present invention includes a pH sensor unit for non-contact measurement of the pH of a sample fluid using light of a predetermined wavelength band, a peristaltic pump unit for transferring the sample fluid to the pH sensor unit, and the pH sensor unit. And a sensor housing unit accommodating the peristaltic pump unit therein, and a control unit determining the pH of the sample fluid using an output of the pH sensor unit, wherein the pH sensor unit includes a first light-transmitting material through which the sample fluid flows. A flow cell, a first light source module that irradiates light in a visible wavelength band and light in a near-infrared (NIR) wavelength band, respectively, to the sample fluid flowing through the first flow cell, and detects light transmitted through the first flow cell And a first light-receiving element, wherein the control unit determines the pH of the sample fluid by calculating absorbance of light in the visible wavelength band and the absorbance in the near-infrared wavelength band detected by the first light source module and detected by the first light source module. It is characterized by that.

Description

An Integrated Module of Non-contact type Optical Sensors

The present invention relates to a non-contact optical sensor integration module, and more specifically, a plurality of sensors used to monitor the state of an internal solution in real time in a reaction tank such as a water treatment tank, a fermentation tank, and a microorganism culture tank is a flow cell type non-contact optical sensor. In addition to minimizing contamination of the solution inside the reaction tank, it is possible to minimize contamination of the solution inside the reaction tank, and by configuring the plurality of sensors as a single module, it is possible to simultaneously measure multiple factors in real time with a single sample solution. About.

In general, in the bio field or environment field, in order to monitor the state of a solution contained in a reaction tank such as a water treatment tank, a fermentation tank, a microbial culture tank, etc., a solution sample is collected and the pH of the solution and the solution are dissolved using various sensors or reagents outside the reaction tank Factors such as oxygen (DO) or optical density (OD) are measured.

However, in the case of collecting a solution sample and measuring these factors outside the reaction tank, it is not only impossible to monitor the condition of the solution inside the reaction tank in real time. There is a problem that damages the entire solution of.

Therefore, recently, in order to solve the problems of the prior art, electrodes or probe-type sensors for measuring these factors are inserted into the reactor in a sterilized state to monitor the state of the internal solution in real time outside the reactor. However, the configuration of the probe-type sensor and the reaction tank to which it is applied is specifically disclosed in the following [Document 1] and [Document 2].

However, in the case of such probe type sensors, there is an advantage that real-time monitoring inside the reaction tank is possible, but because the sensor probe is in direct contact with the solution, there is a problem that contamination of the solution inside the reaction tank may still occur when the sensor is installed or replaced. have.

In addition, in the case of the probe type sensors, since the water flow generated inside the reaction tank by the stirrer is interfered by the outer surface of the probe having a large diameter in order to make the reaction inside the reaction tank uniform, a plurality of sensor probes are used to measure a plurality of factors. When installed inside the reaction tank, there is also a problem in that it is difficult to achieve a uniform reaction throughout the reaction tank.

[Document 1] Korean Patent Registration No. 10-0977067 (published on July 29, 2009)

[Document 2] US Patent Publication No. 2019-0153381 (published on May 23, 2019)

The present invention is to solve the problems of the prior art as described above, an object of the present invention is a plurality of sensors used to monitor the state of the internal solution in real time in a reaction tank such as a water treatment tank or a bioreactor is a flow cell type The non-contact optical sensor not only minimizes contamination of the solution inside the reaction tank by being made of a non-contact type optical sensor, but also enables the simultaneous measurement of multiple factors in real time with a single sample solution by configuring the plurality of sensors as a single module. It is to provide an integrated module.

In addition, another object of the present invention is that the pH sensor among the plurality of sensors is configured to measure the absorbance of a solution for light in a plurality of visible wavelength bands and a plurality of near-infrared (NIR) light. It is to provide a non-contact optical sensor integrated module that can minimize measurement errors due to color change.

In order to achieve the above object, the integrated non-contact optical sensor module according to the present invention includes a pH sensor unit for non-contact measurement of the pH of a sample fluid using light of a predetermined wavelength band, and for transferring the sample fluid to the pH sensor unit. A peristaltic pump unit, a sensor housing unit accommodating the pH sensor unit and the peristaltic pump unit therein, and a control unit for determining the pH of the sample fluid by using the output of the pH sensor unit, wherein the pH sensor unit includes: A first flow cell made of a flowing light-transmitting material, a first light source module for irradiating light in a visible wavelength band and light in a near-infrared (NIR) wavelength band, respectively, to the sample fluid flowing through the first flow cell, and the first And a first light-receiving element for detecting light transmitted through the flow cell, and the control unit calculates the absorbance of light in the visible wavelength band and the absorbance of light in the near-infrared wavelength band detected by the first light source module and detected by the first light source module. It is characterized in that the pH of the sample fluid is determined.

In addition, the first light source module includes a plurality of visible light emitting members for irradiating visible light in different wavelength bands and a plurality of near infrared light emitting members for irradiating near infrared rays in different wavelength bands, and the control unit includes a visible light emitting member And the absorbance of the light irradiated by the near-infrared light emitting member and detected by the first light receiving element, respectively, to determine the pH of the sample fluid.

In addition, the first light source module further comprises a disk-shaped light source support member in which the visible light emitting member and the near infrared light emitting member are arranged in a circular shape, and a motor for rotating the light source support member, wherein the controller uses a motor to When any one of the visible light emitting member and the near-infrared light emitting member is positioned to face the first light-receiving element by rotating the light source support member, the light-emitting member is turned on and irradiated from the light-emitting member to be detected by the first light-receiving element. It is characterized in that the absorbance of the light is calculated.

In addition, a through hole is formed at an outer end of the light source support member at a position corresponding to each of the light emitting members, and the first light source module is installed on an outer edge of the light source support member to provide the visible light emitting member and the near-infrared light emitting member. Further comprising a light source position detecting member for detecting a position of, wherein the control unit determines that the light emitting member is positioned to face the first light receiving element when the light source position detecting member detects any one of the through holes. It is characterized.

In addition, the first light source module further includes a light emitting member for an OD sensor that irradiates light of a predetermined wavelength band to the sample fluid flowing through the first flow cell in order to non-contact measurement of the optical density (OD) of the sample fluid. However, the control unit is characterized in that the OD of the sample fluid is determined by calculating the absorbance of light irradiated from the light emitting member for the OD sensor and detected by the first light receiving element.

In addition, the DO sensor unit further includes a DO sensor unit accommodated in the sensor housing unit to non-contact measurement of dissolved oxygen (DO) of the sample fluid using light of a predetermined wavelength band, wherein the DO sensor unit transmits light through which the sample fluid flows. A flow cell for a DO sensor made of material, a phosphor installed inside the flow cell for a DO sensor, a light source module for a DO sensor that irradiates light in the ultraviolet (UV) wavelength band to the phosphor, and irradiated from the light source module for the DO sensor. And a light-receiving element for a DO sensor that detects fluorescence emitted from the phosphor by light, and the control unit determines the DO of the sample fluid by using the intensity of fluorescence detected by the light-receiving element for the DO sensor. .

In addition, an OD sensor unit that is accommodated in the sensor housing unit and measures the optical density (OD) of the sample fluid in a non-contact manner using light of a predetermined wavelength, and the dissolved oxygen (DO) of the sample fluid is measured. Further comprising at least one of the DO sensor unit, wherein the OD sensor unit irradiates light in the near-infrared (NIR) wavelength band to the sample fluid flowing through the second flow cell and the second flow cell made of a light-transmitting material through which the sample fluid flows. And a second light-receiving element configured to detect light transmitted through the second flow cell, and the DO sensor unit comprises a third flow cell made of a light-transmitting material through which a sample fluid flows, and the third flow cell A phosphor installed inside of the phosphor, a third light source module that irradiates the phosphor with light in an ultraviolet (UV) wavelength band, and a third light-receiving device that detects fluorescence emitted from the phosphor by the light irradiated from the third light source module Including, the control unit determines the OD of the sample fluid by calculating the absorbance of the light irradiated from the second light source module and detected by the second light-receiving element, and irradiated from the third light source module in the third light-receiving element. It is characterized in that the DO of the sample fluid is determined using the detected fluorescence intensity.

In addition, the second flow cell and the third flow cell are each connected to the peristaltic pump so that a path through which the sample fluid flows is connected in series or in parallel with a flow path of the sample fluid of the first flow cell. .

In the non-contact optical sensor integrated module according to the present invention, the plurality of sensor units used to monitor the state of the sample fluid in real time are made of flow cell-type non-contact optical sensors, so even when used in a reaction tank such as a bioreactor or fermentation tank, the inside of the reaction tank There is an advantage of minimizing contamination of the solution.

In addition, the non-contact optical sensor integrated module according to the present invention has the advantage of being convenient to use since it is not necessary to separately install each sensor unit in the reaction tank because the plurality of sensor units are accommodated in the sensor housing unit to be configured as a single module.

In addition, the non-contact optical sensor integration module according to the present invention is configured such that the flow paths of each of the plurality of sensor units are connected in series or parallel within the sensor housing unit, so that a plurality of factors can be real-time with one sample fluid drawing. There is an advantage that it is possible to measure at the same time.

In addition, when the non-contact optical sensor integrated module according to the present invention is used in a reaction tank, since only a sterilized thin-diameter tube needs to be inserted into the reaction tank in order to withdraw the sample fluid, the possibility of contamination of the solution inside the reaction tank is greatly reduced. When the internal solution is stirred, resistance to water flow is minimized, so that a uniform reaction can be achieved.

In addition, in the non-contact optical sensor integration module according to the present invention, the pH sensor unit among the plurality of sensor units uses a light source in the visible wavelength band and a light source in the near-infrared wavelength band in combination. Since it is a method of determining the pH of the medium, there is an advantage of minimizing measurement errors due to color change of the medium in fermentation or microbial culture.

1 is a perspective view showing the appearance of a non-contact optical sensor integrated module according to a first embodiment of the present invention;
2 is a view for explaining the internal configuration of the non-contact optical sensor integrated module according to the first embodiment of the present invention;
3 is an enlarged view of the "A" part of FIG. 2;
4 is a perspective view for explaining the detailed configuration of the first light source module shown in FIG. 2;
5 is a block diagram for explaining the operation configuration of the non-contact optical sensor integrated module according to the first embodiment of the present invention, and
6 to 8 are views for explaining the internal configuration of the non-contact optical sensor integration module according to the second to fourth embodiments of the present invention, respectively.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

(Example 1)

1 is a perspective view showing the appearance of a non-contact optical sensor integration module according to a first embodiment of the present invention, and Figure 2 is a view for explaining the internal configuration of the non-contact optical sensor integration module according to the first embodiment of the present invention. .

In addition, Figure 3 is an enlarged view of the "A" part of Figure 2, Figure 4 is a perspective view for explaining the detailed configuration of the first light source module shown in Figure 2, Figure 5 is a first embodiment of the present invention It is a block diagram for explaining the operation configuration of the non-contact optical sensor integrated module according to.

The non-contact optical sensor integrated module according to the present invention includes, for example, a pH sensor unit 20 for non-contact measurement of the pH of a sample fluid, and an OD sensor unit for non-contact measurement of the optical density (OD) of the sample fluid ( 30), and at least one of the DO sensor unit 40 for measuring dissolved oxygen (DO) of the sample fluid. In this embodiment, as an example, all three sensor units are included. It consisted of.

In addition, the sensor units 20, 30, and 40 each measure the pH, OD, and DO of the sample fluid in an optical manner using light in a predetermined wavelength band.

In addition, in this embodiment, as an example, the sensor units 20, 30, and 40 are the flow cells 21, 31 and 41 of the pH sensor unit 20, the OD sensor unit 30, and the DO sensor unit 40, respectively. A case in which the connection tubes 14 are sequentially connected to each other in series will be described, but it goes without saying that the connection order of each sensor unit 20, 30, and 40 may be configured differently.

In addition, the pH sensor unit 20, the OD sensor unit 30, and the DO sensor unit 40 are accommodated in the sensor housing unit 10 to form one module as a whole, the sensor housing unit (10) is a box-shaped housing 11 in which the three sensor units 20, 30, and 40 are accommodated, and a sample inlet tube 12 and a sample discharge tube 13 connected to both sides of the housing 11 Consists of including.

In addition, the sensor housing unit 10 further includes a display module 15 and an input module 16 installed on one side of the housing 11, wherein the input module 16 is provided by the user. A signal for operating the ,40), a timer setting signal, etc. can be input, and the input content and the pH, OD, and DO of the sample fluid measured by each sensor unit 20,30,40 can be input to the display module 15. Etc. may be displayed.

As described above, the integrated non-contact optical sensor module according to the present invention has a plurality of sensors accommodated in the sensor housing unit 10 to be configured as a single module, so it is not necessary to install each sensor unit separately when used in a reaction tank, so it is convenient to use. There is this.

In addition, a peristaltic pump 17 for transferring a sample fluid to each sensor unit 20, 30, and 40 may be further accommodated in the housing 11, and the peristaltic pump 17 includes a sample inlet tube ( The sample fluid is introduced through one side 17a connected to 12), and the sample fluid is transferred to the sensor units 20, 30, and 40 through the other side 17b.

In this case, since the peristaltic pump 17 transfers the sample fluid in a non-contact manner like a conventional peristaltic pump, contamination of the sample fluid generated in the sample drawing step can be minimized.

With the above configuration, the non-contact optical sensor integrated module according to the present invention only needs to be inserted into the reaction tank with a sterilized thin-diameter tube (i.e., sample inlet tube) for withdrawal of sample fluid when used in a reaction tank. The possibility of contamination is greatly reduced, and resistance to water flow is minimized when the solution inside the reaction tank is stirred, so that a uniform reaction can be achieved.

On the other hand, the pH sensor unit 20 is a first flow cell 21 made of a light-transmitting material through which the sample fluid flows, and the sample fluid flowing through the first flow cell 21, respectively, light and near-infrared rays in the visible wavelength band. And a first light source module 22 for irradiating light in a (NIR) wavelength band, and a first light receiving element 23 for detecting light transmitted through the first flow cell 21.

At this time, the first flow cell 21 is made of a material such as optically transparent quartz, and a sample fluid is introduced into one side, and an inlet port connected to the other side 17b of the peristaltic pump 17 by a connection tube 14 (21a) and a discharge port 21b for discharging the introduced sample fluid to the OD sensor unit 30 side are formed.

In addition, the first light source module 22 is installed on an outer side of the first flow cell 21, and the first light receiving element 23 faces the first light source module 22. It is preferable to be installed on the other side of the outside of (21).

In addition, the first light source module 22 includes a plurality of visible light emitting members 22b for irradiating visible light in different wavelength bands and a plurality of near infrared light emitting members 22b ′ for irradiating near infrared rays in different wavelength bands. It is configured to include, and in this embodiment, as an example, the visible light emitting member 22b and the near infrared light emitting member 22b' are each composed of three LEDs.

In addition, the six light-emitting members 22b and 22b' may be configured to be arranged in the longitudinal direction of the first flow cell 21, but in this embodiment, the six light-emitting members 22b and 22b' It was configured to be arranged in a circular shape with respect to the side of the first flow cell 21.

To this end, the first light source module 22 includes a disk-shaped light source supporting member 22a in which the visible light emitting member 22b and the near-infrared light emitting member 22b' are arranged in a circle, and the rotation shaft 22d. It is configured to further include a motor 22e connected to the central portion of the light source supporting member 22a to rotate the light source supporting member 22a.

In addition, a through hole 22c is formed at an outer end of the light source support member 22a at a position corresponding to each of the light emitting members 22b and 22b', and the first light source module 22 includes the light source support member It is configured to further include a light source position detection member 25 installed on the outer edge of (22a) for detecting the positions of the visible light emitting member 22b and the near-infrared light emitting member 22b'.

At this time, the light source position detection member 25 is a support (25a) surrounding the outer rim of the light source support member (22a) in an approximately'C' shape, and each of the supporters (25a) installed on opposite sides of each other. It comprises a transmitter (25b) and a receiver (25c).

According to the above configuration, the light source position sensing member 25 detects the position of the corresponding light emitting members 22b and 22b' when a signal from the transmitter 25b is received by the receiver through the through hole 22c. , When the light source position sensing member 25 detects the position of any one of the light emitting members 22b, 22b', the corresponding light emitting member 22b, 22b' has a position facing the first light-receiving element 23 It is desirable to be installed as much as possible.

Next, the OD sensor unit 30 transmits light in the near-infrared (NIR) wavelength band to the sample fluid flowing through the second flow cell 31 and the second flow cell 31 made of a light-transmitting material through which the sample fluid flows. A second light source module 32 to irradiate, and a second light-receiving element 33 for detecting light transmitted through the second flow cell 31.

At this time, the second flow cell 31 is made of a material such as optically transparent quartz, and a sample fluid is introduced into one side and is connected to the outlet 21b of the first flow cell 21 by a connection tube 14 An inlet 31a to be used and an outlet 31b for discharging the introduced sample fluid to the DO sensor unit 40 are formed.

In addition, the second light source module 32 is installed on an outer side of the second flow cell 31, and the second light receiving element 33 faces the second light source module 32. It is preferable to be installed on the other side of the outside of (31).

In addition, the DO sensor unit 40 includes a third flow cell 41 made of a light-transmitting material through which the sample fluid flows, a phosphor 44 installed inside the third flow cell 41, and the phosphor 44. A third light source module 42 that irradiates light in an ultraviolet (UV) wavelength band, and a third light-receiving device that detects fluorescence emitted from the phosphor 44 by the light irradiated from the third light source module 42 It is composed of (43).

At this time, the second flow cell 41 is made of a material such as optically transparent quartz, and a sample fluid is introduced into one side and is connected to the outlet 31b of the second flow cell 31 by a connection tube 14 A discharge port 41b to which the sample discharge tube 13 is connected is formed so as to discharge the inlet 41a and the introduced sample fluid to the outside.

In addition, the third light source module 42 is installed on an outer side of the third flow cell 41, and the third light receiving element 43 faces the third light source module 42. It is preferable to be installed on the other side outside of (41).

In addition, the phosphor 44 is preferably installed in the direction in which the third light-receiving element 43 is installed (that is, the direction opposite to the third light source module), and the DO sensor unit 40 is Is a cut-on filter 45 that removes light in the near-infrared (NIR) wavelength band on the outer surface of the third flow cell 41 on which the phosphor 44 is installed so that only fluorescence is continuously detected by the third light-receiving device 43. ) May be installed.

The non-contact optical sensor integration module according to the present embodiment configured as described above is composed of a plurality of sensor units used to monitor the state of the sample fluid in real time as a flow cell-type non-contact optical sensor, so that it can be used in a reaction tank such as a bioreactor or a fermentation tank. Even when used, there is an advantage of minimizing contamination of the solution inside the reaction tank.

Looking at the operation configuration of the integrated non-contact optical sensor module according to the present embodiment configured as described above, when an operation signal is input from a user through the input module 16, the control unit 100 is stored in a program previously stored in the memory unit 140. Accordingly, the operation of each sensor unit 20, 30, 40 and the peristaltic pump 17 is controlled.

In this case, the control unit 100 operates the light source modules 22, 32, and 42 of each sensor unit, and for the pH sensor unit 20, the operation of the motor 22e and the light source position detection member 25 is also controlled. It is done.

In this way, when the light source modules 22, 32 and 42 of each sensor unit 20, 30, 40 are operated, the control unit 100 outputs the output of each sensor unit, that is, the output of each light receiving element 23, 33, 43 The pH, DO, and OD of the sample fluid are determined through the pH calculation unit 160, the DO calculation unit 170, and the OD calculation unit 180, respectively.

In addition, the control unit 100 may display the determined pH, DO, and OD of the sample fluid through the display module 15.

In this embodiment, a case in which the pH calculation unit 160, the DO calculation unit 170, and the OD calculation unit 180 are separately provided is described as an example. It goes without saying that the pH calculation unit 160, the DO calculation unit 170, and the OD calculation unit 180 may be integrated into the control unit 100 and configured as necessary.

First, in relation to the pH sensor unit 20, the control unit 100 irradiates the first light source module 22 through the pH calculation unit 160 and detects the visible light wavelength detected by the first light-receiving device 23. The pH of the sample fluid is determined by calculating the absorbance of the band light and the absorbance of the near-infrared wavelength band.

More specifically, the control unit 100 calculates the absorbance of light detected by the first light-receiving element 23 by being irradiated from a plurality of visible light emitting members 22b and a plurality of near-infrared light emitting members 22b'. The pH of the sample fluid is determined.

In this case, the control unit 100 rotates the light source support member 22a by using the motor 22e of the first light source module 22 so that the visible light emitting member 22b and the near infrared light emitting member 22b' When any one of them is positioned to face the first light-receiving element 23, the corresponding light-emitting members 22b and 22b' are turned on to be irradiated from the corresponding light-emitting members 22b and 22b', and the first light-receiving element 23 ), the absorbance of the detected light is calculated.

In addition, when the control unit 100 detects any one of the through holes 22c formed in the light source support member 22a, the light emitting member 22b, 22b' is It is determined that it is positioned so as to face the light-receiving element 23.

The light emitting members 22b of the plurality of visible light wavelength bands used in the pH sensor unit 20 according to the present invention are for detecting the color change of the sample fluid due to fermentation, etc., and determine the actual pH of the plurality of sample fluids. To measure.

In general, when the color of the sample fluid changes in the visible light wavelength band due to fermentation or the like, the absorbance in the visible light wavelength band changes even at the same pH, which also affects the absorbance in the near infrared wavelength band.

Therefore, in the present invention, the absorbance detected for the light emitting member 22b in the visible wavelength band and the absorbance detected for the light emitting member 22b' in the near infrared wavelength band in order to correct the measurement error due to the color change of the solution. It is characterized in that the pH of the sample fluid is determined by using the correlation therebetween.

To this end, the memory unit 140 includes relationship information between pH and absorbance in the visible light wavelength band corresponding to each pH value, relationship information between pH and absorbance in the near-infrared wavelength band, and when color change of the solution occurs. It is preferable that information on the pH correlation between the absorbance in the visible wavelength band and the absorbance in the near infrared wavelength band is stored in a DB format.

With the configuration as described above, the integrated non-contact optical sensor module according to the present invention has the advantage that the pH sensor among the plurality of sensor units can minimize measurement errors due to color change of the medium in fermentation or microbial culture.

On the other hand, the control unit 100 for the OD sensor unit 30, similarly to the pH sensor unit 20 described above, irradiated by the second light source module 32 to determine the absorbance of the light detected by the second light receiving element 33. It is calculated to determine the OD of the sample fluid. In this case, it is preferable that the memory unit 140 or the like stores information on the relationship between the OD and the absorbance for the wavelength band of the second light source module 32.

In addition, the control unit 100 uses the intensity of fluorescence irradiated by the third light source module 42 to the DO sensor unit 40 and detected by the third light receiving device 43 to determine the DO of the sample fluid. In this case, it is preferable that the memory unit 140 or the like store relationship information between the DO and fluorescence intensity for the wavelength band of the third light source module 42.

(Second Example)

6 is a view for explaining the internal configuration of the non-contact optical sensor integrated module according to the second embodiment of the present invention.

In the first embodiment described above, the pH sensor unit 20, the OD sensor unit 30, and the DO sensor unit 40 are connected in series with each other, but in the second embodiment, these sensor units 20, 30, 40 Since there is a difference only in the fact that) are connected in parallel with each other, a redundant description of the configuration and measurement method of each sensor unit will be omitted.

As described in the first and second embodiments, the non-contact optical sensor integration module according to the present invention includes the peristaltic pump so that the flow cells of each of the plurality of sensor units are connected in series or parallel to each other in the sensor housing unit. Since it is connected to, it is possible to simultaneously measure multiple factors in real time with only one sample fluid withdrawal.

(Example 3 and Example 4)

7 and 8 are diagrams for explaining the internal configuration of the non-contact optical sensor integration module according to the third and fourth embodiments of the present invention, respectively. In the third and fourth embodiments, each sensor unit is in series. Since there is a difference only in connection with or in parallel connection, only the third embodiment will be described here in order to prevent redundant description.

The non-contact optical sensor integration module according to the third embodiment of the present invention is characterized in that the integrated sensor unit 50 and the DO sensor unit 40 are connected in series with each other as shown in FIG. 50) is characterized in that a common pH sensor unit 20 and an OD sensor unit 30 are combined in one integrated sensor unit 50 in that it is an absorbance method.

In this case, the fourth light source module 52 is a combination of the second light source module 32 to the configuration of the first light source module 22 described above, and specifically, the light emitting members 22b and 22b of the first light source module 22 ') and the second light source module 32 are arranged in a circular shape on the light source support member 52a.

At this time, since the configurations of the through hole 52c, the rotation shaft 52d, the motor 52e, and the light source position sensing member 55 are the same as those of the first embodiment described above, a redundant description will be omitted.

According to the above configuration, the non-contact optical sensor integrated module according to the third and fourth embodiments of the present invention includes the pH sensor unit 20 and the OD sensor unit 30 applying the same absorbance method as a single integrated sensor unit. Since it is an integrated configuration of 50, there is an advantage that the configuration of the sensor module is further downsized.

10: sensor housing part 20: pH sensor part
30: OD sensor unit 40: DO sensor unit
50: integrated sensor unit

Claims (8)

A pH sensor unit for non-contact measurement of the pH of the sample fluid using light in a predetermined wavelength band;
DO sensor unit for non-contact measurement of dissolved oxygen (DO) of the sample fluid using light of a predetermined wavelength band;
A peristaltic pump unit for transferring the sample fluid to the pH sensor unit and the DO sensor unit;
A sensor housing unit accommodating the pH sensor unit, the DO sensor unit, and the peristaltic pump unit therein; And
Including a control unit for determining the pH and DO of the sample fluid using the output of the pH sensor unit and the DO sensor unit,
The pH sensor unit includes a first flow cell made of a light-transmitting material through which a sample fluid flows, and an agent for irradiating light in a visible wavelength band and light in a near-infrared (NIR) wavelength band to the sample fluid flowing through the first flow cell, respectively. 1 light source module, and a first light-receiving element for detecting the light transmitted through the first flow cell,
The DO sensor unit includes a flow cell for a DO sensor made of a light-transmitting material through which a sample fluid flows, a phosphor installed inside the flow cell for a DO sensor, and a light source for a DO sensor that irradiates the phosphor with light in an ultraviolet (UV) wavelength band. A module, and a DO sensor light receiving element for detecting fluorescence emitted from the phosphor by the light irradiated from the DO sensor light source module,
The control unit determines the pH of the sample fluid by calculating the absorbance of light in the visible wavelength band and the absorbance in the near-infrared wavelength band detected by the first light-receiving device by being irradiated from the first light source module, and determining the pH of the sample fluid. A non-contact optical sensor integrated module, characterized in that the DO of the sample fluid is determined using the intensity of fluorescence. The method of claim 1,
The first light source module includes a plurality of visible light emitting members for irradiating visible light in different wavelength bands and a plurality of near infrared light emitting members for irradiating near infrared rays in different wavelength bands,
The control unit is a non-contact optical sensor integrated module, characterized in that to determine the pH of the sample fluid by calculating the absorbance of the light irradiated from the visible light emitting member and the near-infrared light emitting member and detected by the first light receiving element, respectively. The method of claim 2,
The first light source module,
Further comprising a disk-shaped light source supporting member in which the visible light emitting member and the near-infrared light emitting member are arranged in a circle, and a motor for rotating the light source supporting member,
The control unit rotates the light source support member using a motor so that when any one of the visible light emitting member and the near infrared light emitting member is positioned to face the first light receiving element, the corresponding light emitting member is turned on and irradiated from the corresponding light emitting member. And calculating the absorbance of the light detected by the first light-receiving element. The method of claim 3,
A through hole is formed at a position corresponding to each of the light emitting members at an outer end of the light source support member,
The first light source module further comprises a light source position detection member installed on the outer edge of the light source support member to detect the positions of the visible light emitting member and the near-infrared light emitting member,
Wherein the control unit determines that the light emitting member is positioned to face the first light receiving element when the light source position detecting member detects any one of the through holes. The method according to any one of claims 2 to 4,
The first light source module further includes a light emitting member for an OD sensor that irradiates light of a predetermined wavelength band to the sample fluid flowing through the first flow cell in order to non-contact measurement of the optical density (OD) of the sample fluid,
The control unit is a non-contact optical sensor integrated module, characterized in that to determine the OD of the sample fluid by calculating the absorbance of the light irradiated from the light emitting member for the OD sensor and detected by the first light-receiving element. delete The method according to any one of claims 1 to 4,
Further comprising an OD sensor unit that is accommodated in the sensor housing unit and measures the optical density (OD) of the sample fluid in a non-contact manner, respectively, using light of a predetermined wavelength,
The OD sensor unit includes a second flow cell made of a light-transmitting material through which a sample fluid flows, a second light source module irradiating light in a near-infrared (NIR) wavelength band to the sample fluid flowing through the second flow cell, and the second flow cell. And a second light-receiving element for detecting light that has passed through,
The control unit is a non-contact optical sensor integrated module, characterized in that to determine the OD of the sample fluid by calculating the absorbance of the light irradiated from the second light source module and detected by the second light receiving element. The method of claim 7,
The second flow cell and the flow cell for the DO sensor are each connected to the peristaltic pump so that a path through which the sample fluid flows is connected in series or in parallel with a flow path of the sample fluid of the first flow cell. Optical sensor integration module.

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