KR102242980B1 - Antibacterial nanostructure and method for making the same - Google Patents

Abstract

The present invention relates to a nano-structure which effectively removes or reduces bacteria. The nano-structure comprises: a nano-structure layer including a surface and a plurality of nano-pillars protruding from the surface; and a functional layer consisting of an ionic polymer formed on the nano-structure layer.

Description

Antimicrobial nanostructure and its manufacturing method {ANTIBACTERIAL NANOSTRUCTURE AND METHOD FOR MAKING THE SAME}

The present invention relates to an antimicrobial nanostructure capable of effectively preventing bacterial infection, a method for manufacturing the same, and further to an artificial lens using the same.

Bacteria are single-celled microorganisms that are found in almost all environments and do not cause disease inside or outside the body, but some bacteria enter the human body and multiply rapidly, destroying the body's immunity, causing various diseases or producing toxins. This can cause damage to the human body.

In particular, in the medical field, the presence of bacteria in the operating room can lead to surgical site infection (SSI), so strict infection control for bacteria is usually performed in the operating room. In this regard, you can refer to the Global Guidelines for the Prevention of Surgical Site Infection published in 2016 by the WHO.

In general, with regard to the pathogenesis of surgical site infection, i) disinfect the patient's surgical site during surgery to prevent internal sources of infection caused by the patient's own flora, and use prophylactic antibiotics on the patient during surgery, and ii) In order to prevent external sources of infection caused by the surgical environment such as surgical instruments, operating room, and operating staff, disinfection of surgical instruments, ventilation of the operating room, disinfection of the hands of the operating staff, and wearing a surgical mask that completely covers the mouth and nose of the operating staff are performed. Since bacteria in the operating room cannot be completely removed even by the measures, the presence of bacteria is reported in the operating room, although the concentration is low, and surgical site infection occurs.

In eye surgery, surgical site infection can cause loss of vision or decreased vision, and the risk is very high. Moreover, in the ocular immune system, the eye is relatively vulnerable to immunity because there are no lymphatic vessels inside the eye, the cornea is exposed to the outside, and the immunity of the eye is separated from other parts of the human body.

In this regard, in addition to the above-described measures, there is a need to impart antimicrobial properties to the intraocular lens itself in addition to the above-described measures in preparation for the possibility of infection of the surgical site by bacteria, especially in the case of an intraocular lens inserted into the eye during eye surgery.

The present invention, a surface, and a nanostructure layer comprising a plurality of nanopillars protruding from the surface; And it is to provide an antimicrobial nanostructure and a method for manufacturing the same, including a functional layer made of an ionic polymer formed on the nanostructure layer, and further to provide an invention using the same, such as an artificial lens.

A nanostructure according to an embodiment of the present invention is provided. The nanostructure includes a surface and a nanostructure layer including a plurality of nanopillars protruding from the surface; And a functional layer made of an ionic polymer formed on the nanostructure layer.

According to another feature of the present invention, the ionic polymer may be formed by polymerization of an anionic monomer and an anionic monomer.

According to another feature of the present invention, the ionic polymer is p(VBC-co-DMAEMA), p(VBC-co-DMAMAS), p(VBC-co-VIDZ), p(VBC-co-4VP), p (MA-co-DMAEMA), p(MA-co-DMAMAS), p(MA-co-VIDZ), p(MA-co-4VP), p(GMA-co-DMAEMA), p(GMA-co- DMAMAS), p(GMA-co-VIDZ), p(GMA-co-4VP), p(CEA-co-DMAEMA), p(CEA-co-DMAMAS), p(CEA-co-VIDZ), p( CEA-co-4VP) may include at least one.

According to another feature of the present invention, the ratio of VBC:DMAEMA may be 1:1 to 1:10.

According to another feature of the present invention, the cationic monomer is dimethylaminoethyl methacrylate (DMAEMA), 2-dimethylaminomethyl styrene (DMAMAS), n-vinylimidazole (n- It may contain at least one of vinylimidazole, VIDZ), and 4-vinyl pyridine (4VP).

According to another feature of the present invention, the anionic monomer is vinyl benzyl chloride (VBC), maleic anhydride (MA), glycidyl methacrylate (GMA), It may contain at least one of 2-chloroethyl acrylate (CEA).

According to another feature of the present invention, the plurality of nanopillars may be made of an elastic polymer.

According to another feature of the present invention, the plurality of nanopillars are arranged at intervals of 0.2 μm to 2 μm, and the plurality of nano pillars may have a diameter of 50 nm to 1000 nm, and a height of 0.1 to 2 μm. .

An artificial lens comprising a nanostructure according to another embodiment of the present invention is provided. The intraocular lens includes an optical portion and a haptic portion coupled to an outer peripheral portion of the optical portion to fix the optical portion in the eyeball.

According to another feature of the present invention, the nanostructure may be coupled to the artificial lens along the outer periphery of the optical part.

According to another feature of the present invention, it may be coupled on an edge surface horizontal to the optical axis of the artificial lens.

A method of manufacturing a nanostructure according to another embodiment of the present invention is provided. The method includes providing a nanostructure comprising a plurality of nanopillars to a processing chamber; Introducing a vapor phased cationic monomer, an anionic monomer, and an initiator into the processing chamber; Activating the initiator introduced into the processing chamber; And forming an ionic polymer functional layer on the plurality of nanopillars by using the activated initiator.

According to another feature of the present invention, the cationic polymer is DMAEMA, the anionic polymer is VBC, the initiator is TBPO, and the ratio of VBC:DMAEMA may be 1: 1 to 1: 10.

According to another feature of the present invention, the thickness of the functional layer formed on the nanostructure may be 10 ㎚ to 100 ㎚.

According to the antimicrobial nanostructure disclosed in the present specification and a method for manufacturing the same, the nanostructure includes a surface, and a nanostructure layer including a plurality of nanopillars protruding from the surface; And by including a functional layer made of an ionic polymer formed on the nanostructure layer, through a very strong interaction between the nanostructure and bacteria, by fixing the bacteria to the nanostructure to create a nutrient-poor conditions or rupture bacteria (rupture ), effectively removing or reducing bacteria.

The effects according to the present invention are not limited by the contents exemplified above, and more various effects are included in the present specification.

1 is an exemplary perspective view of a nanostructure according to an embodiment of the present invention.
2 is an exemplary cross-sectional view of a nanostructure according to an embodiment of the present invention.
3 is an exemplary cross-sectional view of a nanostructure according to various embodiments of the present invention.
4 and 5 are XPS analysis results of nanostructures before and after formation of a polymerized functional layer by iCVD according to an embodiment of the present invention.
6 and 7 are live/dead staining analysis results of nanostructures before and after formation of a polymerized functional layer by iCVD according to an embodiment of the present invention.
8 is an SEM photograph of a nanostructure according to an embodiment of the present invention.
9 is a photograph of a result of verifying the antibacterial effect of a nanostructure according to an embodiment of the present invention.
10 is a flowchart of a method of manufacturing a nanostructure according to an embodiment of the present invention.
11 shows the structure of an artificial lens according to an embodiment of the present invention.
12 is an exemplary diagram of an application for an artificial lens of a nanostructure according to various embodiments of the present invention.

Advantages and features of the present invention, and a method of achieving them will become apparent with reference to the embodiments described below in detail together with the accompanying drawings. However, the present invention is not limited to the embodiments disclosed below, but will be implemented in a variety of different forms, and only the embodiments make the disclosure of the present invention complete, and common knowledge in the technical field to which the present invention pertains. It is provided to completely inform the scope of the invention to the possessor, and the invention is only defined by the scope of the claims.

In this specification, unless expressly stated to the contrary, "at least one of "A or (or) B," "A and/or (and/or) B," or "one or more of A or/and B The expression "and the like" may be understood to refer to the inclusion of all possible combinations of items listed as non-exclusive. For example, “or B” may be understood to include all cases of i) A, ii) B, and iii) A and B, and “at least one of A and B” and “at least one of A or B” One" may be understood to include both cases of i) at least one A, ii) at least one B, and iii) at least one A and at least one B.

In the present specification, expressions such as "have," "may have," "include," or "may include" mean the existence of a corresponding feature, and do not exclude the presence of an additional feature.

In the present specification, expressions such as "first," "second," "first," or "second," may modify related various elements, regardless of order and/or importance, and one element Is used to distinguish it from other components, but does not limit the components.

In this specification, the same reference numerals refer to the same constituent elements.

In the present specification, each of the features of the various embodiments can be partially or entirely combined or combined with each other, and various interlocking and driving are technically possible, as can be sufficiently understood by a person skilled in the art, and each embodiment is independent from each other. It may be possible to implement it as or it may be possible to implement it together in a related relationship.

Hereinafter, various embodiments of the present invention will be described in detail with reference to the accompanying drawings.

1 is an exemplary perspective view of a nanostructure 100 according to an embodiment of the present invention. 2 is an exemplary cross-sectional view of a nanostructure 100 according to an embodiment of the present invention.

1 and 2, a nanostructure 100 according to an embodiment of the present invention includes a nanostructure layer 110 and a functional layer 120.

The nanostructure layer 110 includes a surface 112 and a plurality of nanopillars 114 protruding from the surface 112.

In FIG. 1, the plurality of nano-pillars 114 are shown to have a cylindrical shape in which the top portion is rounded, but in various embodiments of the present invention, the shape of each of the plurality of nano-pillars 114 is not limited thereto. , All shapes having a surface for forming the functional layer 120 on the plurality of nanopillars 114 may be included. For example, the plurality of nanopillars 114 may have various shapes such as a cone, a cylinder, a rod, a tube, etc. as a whole, and an upper portion thereof may be formed to be round or angled. These various shapes are also referred to as nanowires, nanorods, nanoneedles, nanocones, and nanothrones.

The plurality of nanopillars 114 may be made of an elastic polymer. Since the elastomer has elasticity, when the plurality of nanopillars 114 are deformed, the plurality of nanopillars 114 have a restoring force.

The plurality of nanopillars 114 may have a suitable diameter, height, and arranged spacing to reduce or eliminate bacteria. For example, the plurality of nanopillars 114 may have a diameter of 50 nm to 1,000 nm, and preferably may have a diameter of 200 nm to 500 nm. The plurality of nanopillars 114 may have a height of 0.1 μm to 2 μm, and preferably may have a height of 0.5 to 1.5 μm. The plurality of nanopillars 114 may be arranged with an interval of 0.2 μm to 2 μm. In the present specification, a plurality of nanopillars 114 are aggregated with a specific arrangement, referred to as a nanopillar array.

In various embodiments, the plurality of nanopillars 114 may be arranged to have the same diameter and height with each other. Meanwhile, as shown in FIG. 3, the plurality of nanopillars 114 may be arranged to have different diameters or heights from each other. Through this, the shape in which the functional layer 120 to be formed on the plurality of nanopillars 114 is arranged can be varied, as a result, the ends of the functional layer 120 for bacteria or bacterial colonies of more various sizes. Can be easily attached to. At the same time, the shape in which the plurality of nanopillars 114 are arranged may be varied, and thus bacteria fixed to the functional layer 120 formed on the plurality of nanopillars 114 may be more variously modified. As a result, it is possible to cause immobilization and very large deformation of bacteria or bacterial colonies of various sizes, thereby improving the antimicrobial properties of the nanostructure.

3 is an exemplary cross-sectional view of a nanostructure according to various embodiments of the present invention. For example, FIG. 3(a) shows an embodiment in which a plurality of nanopillars 114 are arranged with a height increasing in a specific direction in one cross-section. 3(b) shows an embodiment in which the height of the nanopillars 114 arranged on the outside in one cross-section is lower than the height of the nanopillars arranged on the inside. 3(c) shows an embodiment in which the height of the nanopillars 114 arranged on the inside in one cross-section is lower than the height of the nanopillars arranged on the outside.

The functional layer 120 formed on the nanostructure 110 may be partially or entirely made of an ionic polymer. The functional layer 120 has a polarity through the ionic polymer, and in particular, can have a very strong interaction with bacteria, through which bacteria can be effectively removed or reduced. The antimicrobial properties of the functional layer 120 related thereto will be described later. In addition, the functional layer 120 may have a suitable thickness to effectively remove or reduce bacteria. For example, the functional layer 120 may be formed on the nanostructure to have a thickness of 10 nm to 100 nm.

In various embodiments of the present invention, the ionic polymer of the functional layer 120 is obtained by polymerization of a cationic monomer and an anionic monomer by chemical vapor deposition (iCVD) using an initiator. Can be made. Cationic monomers include dimethylaminoethyl methacrylate (DMAEMA), 2-dimethylaminomethyl styrene (DMAMAS), n-vinylimidazole (VIDZ), and 4-vinyl pyridine. (4-vinyl pyridine, 4VP) including at least one of, but is not limited thereto. Anionic monomers include vinyl benzyl chloride (VBC), maleic anhydride (MA), glycidyl methacrylate (GMA), and 2-chloroethyl acrylate (2-Chloroethyl). acrylate, CEA), but is not limited thereto. Through polymerization of cationic and anionic monomers, ionic polymers p(VBC-co-DMAEMA), p(VBC-co-DMAMAS), p(VBC-co-VIDZ), p(VBC-co-4VP) ), p(MA-co-DMAEMA), p(MA-co-DMAMAS), p(MA-co-VIDZ), p(MA-co-4VP), p(GMA-co-DMAEMA), p(GMA -co-DMAMAS), p(GMA-co-VIDZ), p(GMA-co-4VP), p(CEA-co-DMAEMA), p(CEA-co-DMAMAS), p(CEA-co-VIDZ) , p(CEA-co-4VP) may be formed.

In the formed ionic polymer p (VBC-co-DMAEMA), the ratio of VBC:DMAEMA is 1:1 to 1:10, preferably 1:2 to 1:6, and more preferably 1:4.

4 and 5 are XPS analysis results of nanostructures before and after formation of a polymerized functional layer by iCVD according to an embodiment of the present invention.

Specifically, FIG. 4 and FIG. 5 show “a functional layer made of an ionic polymer p (DMAEMA-co-VBC) polymerized by iCVD at a ratio of VBC:DMAEMA on the plurality of nanopillars 114 at 1:4. XPS (X-ray photoelectron spectroscopy) analysis result of the nanostructure 100 before and after 120 is formed” is shown.

As a result of XPS analysis, in the process of polymerization of the ionic polymer p (VBC-co-DMAEMA) on a plurality of nanopillars 114 through a change in binding energy, nitrogen cations (N+) that were not present before polymerization ) Is generated, and accordingly, it can be confirmed that the ionic polymer p (VBC-co-DMAEMA) has a positive charge.

6 and 7 are live/dead staining analysis results of nanostructures before and after formation of a polymerized functional layer by iCVD according to an embodiment of the present invention.

Specifically, FIGS. 6 and 7 show “function consisting of ionic polymer p (VBC-co-DMAEMA) polymerized by iCVD at a ratio of VBC:DMAEMA of 1:4 on a plurality of nanopillars 114 Before and after the layer 120 is formed, after invading these nanostructures in a culture medium at 37° C. for 24 hours, corneal endothelial cells, which are human cells, were cultured for 24 hours using the culture medium and confirmed live/ Dead staining analysis results” are shown.

As a result of the live/dead staining analysis, referring to the fluorescence analysis image (FIG. 6) distinguishing live corneal endothelial cells from dead corneal epithelial cells in the culture medium in the nanostructure 100 before and after the functional layer 120 is formed. , A decrease in the number of live corneal endothelial cells was not confirmed, and the viability of corneal epithelial cells calculated after the functional layer 120 was formed is that of the corneal epithelial cells calculated before the functional layer 120 was formed. It is confirmed that there is almost no difference in survival rate. Accordingly, it is confirmed that the functional layer 120 has no or minimizes side effects on human cells such as toxicity that reduces the viability of corneal epithelial cells.

Due to the plurality of nano-pillars 114 included in the nano-structure layer 110 of the nano-structure 100 and the functional layer 120 formed on the plurality of nano-pillars 114, side effects to human cells are reduced. Bars that are not present or minimized, and bacteria are effectively removed or reduced, hereinafter, when the nanostructure 100 is exposed to bacteria, a process in which antibacterial is performed will be described.

Antimicrobial properties of nanostructures

Bacteria are classified into gram-positive bacteria and gram-negative bacteria according to the type of cell wall. The cell wall of Gram-positive bacteria has a negative charge due to choic acids, and the cell wall of Gram-negative bacteria has a negative charge due to lipopolysaccharide.

In contrast, the functional layer 120 is made of an ionic polymer, and when the ionic polymer is formed to have a positive charge, there is an electrostatic charge between the cell walls of Gram-positive bacteria and Gram-negative bacteria having a negative charge and the functional layer 120 having a positive charge. Electrostatic attraction occurs, and by a very strong interaction by the electrostatic attraction, the functional layer 120 attracts bacteria very strongly and the bacteria are fixed to the surface of the functional layer 120.

When the bacteria are fixed on the functional layer 120, colonization of bacteria is reduced, and movement of the bacteria is restricted, so that the bacteria are placed in a state of lack of nutrition. In addition, the functional layer 120 is a very strong interaction with the fixed bacteria, causing a very large deformation of the shape (morphology), such as flattened (flattened) or stretched (stretched) of the fixed bacteria.

In addition, since the functional layer 120 is formed on the plurality of nano-pillars 114, a very strong interaction between the functional layer 120 and the bacteria also causes deformation of the plurality of nano-pillars 114. Accordingly, the plurality of nanopillars 114 made of elastomers apply a resilience force by elasticity to the fixed bacteria, and the resilience force by the applied elasticity makes the shape of the fixed bacteria larger, and accordingly, the bacteria It can be deformed beyond the critical point for deformation of the shape, resulting in rupture.

8 is an SEM photograph of a nanostructure according to an embodiment of the present invention. Specifically, FIG. 8 shows a functional layer 120 made of an ionic polymer p (VBC-co-DMAEMA) polymerized by iCVD with a ratio of VBC:DMAEMA of 1:4 on the plurality of nanopillars 114. ) Is formed, and then'Scanning Electron Microscope (SEM) photo' of the nanostructure 100 exposed to S. aureus is shown. In FIG. 8, "Staphylococcus aureus fixed on the nanostructure 100 and deformed, as well as Staphylococcus aureus ruptured beyond the critical point for deformation of the shape" are confirmed.

9 is a photograph of a result of verifying the antibacterial effect of a nanostructure according to an embodiment of the present invention. Specifically, in FIG. 9,'In the nanostructure before the functional layer 120 is formed, 493 Staphylococcus aureus colonies are found when cultured for 24 hours after 30 minutes of application of Staphylococcus aureus. In the case of culturing for 24 hours after 30 minutes of application of Staphylococcus aureus, only one or less colonies of Staphylococcus aureus were identified, and it was confirmed that the nanostructure having the functional layer 120 formed with 99% or more has excellent antibacterial properties.

In addition, the nanostructure 100 according to the present invention causes a very strong interaction with both Gram-positive bacteria and Gram-negative bacteria due to electrostatic attraction, so that all of them can be effectively removed or reduced.

The functional layer 120 has antimicrobial properties against both Gram-positive bacteria and Gram-negative bacteria, without side effects of lowering the viability of human cells, so that the functional layer 120 has an antigen-antibody reaction (antigen In contrast to the -antibody reaction), antibodies can have antimicrobial properties against both Gram-positive bacteria and Gram-negative bacteria without using various antibodies. In addition, the functional layer 120 has a difference in antibiotic susceptibility between Gram-positive bacteria and Gram-negative bacteria, so unlike using different antibiotics and other antibacterial methods, it has antimicrobial properties against both Gram-positive bacteria and Gram-negative bacteria. Can have.

For example, the functional layer 120 has antimicrobial properties against both Gram-positive bacteria Staphylococcus epidermidis and Gram-negative bacteria Pseudomonas aeruginosa, which are the major causes of Pseudomonas aeruginosa after eye surgery. I can have it.

Preparation of antimicrobial nanostructures

In various embodiments of the present invention, the functional layer 120 of the nanostructure 100 may be formed by chemical vapor deposition (iCVD) using an initiator. iCVD is a more conformal and high-purity evaporation, and because of its low processing temperature, it is possible to evaporate even on chemically and physically weaker materials.In particular, an artificial lens made of a relatively weak material that directly contacts the eye, is inserted into the eyeball. It may be advantageous in the manufacture of nanostructures that can be used for vision correction lenses, hard lenses, soft lenses, color lenses, and the like.

10 is a flowchart of a method of manufacturing a nanostructure according to an embodiment of the present invention. First, a nanostructure 100 comprising a plurality of nanopillars 114 is provided in a process chamber (step 1010). Next, a vapor phased cationic monomer, an anionic monomer, and an initiator are introduced into the process chamber (step 1020). Next, the introduced initiator is activated (step 1030). Finally, the functional layer 120 is formed (deposited) on the nanostructure 110 using the activated initiator (step 1040).

In various embodiments of the present invention, the types and polymerization ratios of the cationic monomer and the anionic monomer are as described above.

In various embodiments of the present invention, the initiator includes at least one of tert-butyl peroxide (TBPO) and tert-amyl peroxide (TAP), but is not limited thereto.

In various embodiments of the present invention, the thickness of the functional layer 120 formed on the nanostructure may be 10 nm to 100 nm.

In an embodiment of the present invention, the iCVD process conditions in which the nanostructure is manufactured are i) the cationic monomer is DMAEMA, the anionic monomer is VBC, the initiator is TBPO, and ii) the ratio of VBC:DMAEMA is 1:4. It may be vaporized and introduced into the processing chamber.

Applications for artificial intraocular lenses, etc.

An intraocular lens (IOL) is inserted to replace the lens after removal of the lens in eye surgery. In various embodiments of the present invention, the nanostructure may be applied to the artificial lens in order to impart antimicrobial properties to the artificial lens itself. To this end, the nanostructure can be bonded to the artificial lens in various ways.

11 shows a structure of an artificial intraocular lens 1100 according to an embodiment of the present invention. Referring to FIG. 11, the intraocular lens 1100 is an optical unit (optic, 1110) that plays a role of the lens when inserted into the eye, and the position of the intraocular lens 1100 when inserted into the eye is fixed to the lens pocket or optically. It may include a haptic part (haptic) 1120 for fixing the optical axis of the part 1100 to the eyeball. Referring to FIG. 11, the haptic part 1120 is coupled to the optical part 1110 at the outer periphery of the optical part 1110 and has a shape extending in opposite directions, but the shape of the haptic part 1120 is, Without being limited thereto, it may have various other shapes suitable for fixing the intraocular lens 1100 to the lens pocket or fixing the eyeball to the optical axis of the optical part 1110.

12 illustrates an application in which the nanostructure 100 is coupled to the artificial lens 1100 in various ways, according to various embodiments of the present invention. In FIG. 12, the nanostructure 100 is briefly expressed in a simple cylindrical shape, but each of the plurality of nanopillars 114 or the nanopillar array may be formed by different diameters, heights, or arranged intervals. For the arrangement of the plurality of nanopillars 114, reference is made to FIG. 3. The nanostructure 100 may be coupled to at least a portion of the optical portion 1110 or the haptic portion 1120 of the artificial lens 1100. In addition, when the nanostructure 100 is coupled to the optical portion 1110 or the haptic portion 1120 of the artificial lens 1100, it may be coupled to not only a surface perpendicular to the optical axis but also a surface horizontal to the optical axis.

For example, FIG. 12(a) shows an embodiment in which the nanostructure 100 is coupled on a surface perpendicular to the optical axis along the outer periphery of the optical unit 1100. 12(b) shows an embodiment in which the nanostructure 100 is coupled on a surface perpendicular to the optical axis along a part of the outer circumference of the optical unit 1110. 12(c) shows an embodiment in which the nanostructure 100 is coupled on a surface perpendicular to the optical axis along the outer periphery of the haptic part 1120. 12(d) shows an embodiment in which the nanostructure 100 is coupled on a surface perpendicular to the optical axis at a central portion excluding the outer circumferential portion of the haptic portion 1120. 12(e) shows an embodiment in which the nanostructure 100 is bonded on an edge surface horizontal to the optical axis along a part of the outer circumference of the optical unit 1110. 12(f) shows an embodiment in which the nanostructure 100 is coupled on an edge surface horizontal to the optical axis along a portion of the outer circumferential portion of the haptic portion 1120.

On the other hand, the nanostructure according to the present invention can be used not only through applications that are coupled to the artificial lens, but also lenses that directly contact the eyeball such as lenses for vision correction, hard lenses, soft lenses, and contact lenses that are inserted into the eyeball. It can be used for the like. In addition, the nanostructure according to the present invention may be combined with a container for storing lenses or the like in direct contact with the eyeball, and as a result, it is possible to eliminate or reduce the likelihood that a lens or the like in direct contact with the eyeball is exposed to bacteria.

Although the embodiments of the present invention have been described in more detail with reference to the accompanying drawings, the present invention is not necessarily limited to these embodiments, and various modifications may be made without departing from the spirit of the present invention. . Accordingly, the embodiments disclosed in the present invention are not intended to limit the technical idea of the present invention, but to explain the technical idea, and the scope of the technical idea of the present invention is not limited by these embodiments. Therefore, it should be understood that the embodiments described above are illustrative and non-limiting in all respects. The scope of protection of the present invention should be construed by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present invention.

Claims (14)

A nanostructure layer including a surface and a plurality of nanopillars protruding from the surface; And
Including a functional layer made of an ionic polymer formed on a plurality of nanopillars of the nanostructure layer,
The plurality of nanofillers are made of an elastic polymer,
The ionic polymer is formed to have a positive charge by polymerization of a cationic monomer and an anionic monomer,
The plurality of nanopillars are arranged at intervals of 0.2 μm to 2 μm,
The plurality of nano-pillars have a diameter of 50 ㎚ to 1000 ㎚, a height of 0.1 to 2 ㎛, nanostructures. delete The method of claim 1,
The ionic polymer is p(VBC-co-DMAEMA), p(VBC-co-DMAMAS), p(VBC-co-VIDZ), p(VBC-co-4VP), p(MA-co-DMAEMA), p(MA-co-DMAMAS), p(MA-co-VIDZ), p(MA-co-4VP), p(GMA-co-DMAEMA), p(GMA-co-DMAMAS), p(GMA-co -VIDZ), p(GMA-co-4VP), p(CEA-co-DMAEMA), p(CEA-co-DMAMAS), p(CEA-co-VIDZ), p(CEA-co-4VP) Containing one, nanostructures. The method of claim 3,
VBC: DMAEMA ratio of 1: 1 to 1: 10, nanostructures. The method of claim 1,
The cationic monomer is dimethylaminoethyl methacrylate (DMAEMA), 2-dimethylaminomethyl styrene (DMAMAS), n-vinylimidazole (VIDZ), 4-vinyl A nanostructure comprising at least one of pyridine (4-vinyl pyridine, 4VP). The method of claim 1,
The anionic monomer is vinyl benzyl chloride (VBC), maleic anhydride (MA), glycidyl methacrylate (GMA), 2-chloroethyl acrylate (2- A nanostructure comprising at least one of chloroethyl acrylate, CEA). The method of claim 1,
The plurality of nano-pillars are arranged in different diameters or heights, nanostructures. delete An artificial intraocular lens comprising the nanostructure according to claim 1,
The intraocular lens includes an optical part, and a haptic part coupled to an outer peripheral part of the optical part to fix the optical part in the eyeball. The method of claim 9,
The nanostructure is coupled to the artificial lens along the outer periphery of the optical unit, artificial lens. The method of claim 10,
The nanostructure is bonded on a surface horizontal to the optical axis of the artificial lens or on a surface perpendicular to the optical axis, the eye lens. As a method of manufacturing a nanostructure,
Providing a nanostructure including a plurality of nanopillars to the processing chamber;
Introducing a vapor phased cationic monomer, an anionic monomer, and an initiator into the processing chamber;
Activating the initiator introduced into the processing chamber; And
Using the activated initiator, forming a functional layer of an ionic polymer on the plurality of nanostructures,
The plurality of nanopillars are made of an elastomer,
The method, wherein the ionic polymer is formed to have a positive charge by polymerization of the cationic monomer and the anionic monomer. The method of claim 12,
The cationic monomer is DMAEMA, the anionic monomer is VBC, the initiator is TBPO,
The ratio of VBC:DMAEMA is 1:1 to 1:10, the method. The method of claim 12,
The thickness of the functional layer formed on the nanostructure is 10 nm to 100 nm, the method.

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