KR102238139B1 - Garlic skin fermented feeds using Rizofus strains and methods for manufacturing the same - Google Patents

Abstract

The present invention is from 0.1 to 100 parts by weight of a mixture containing 10 to 40% by weight of garlic peel, 10 to 30% by weight of rice bran residue, 10 to 30% by weight of soybean residue, and 20 to 60% by weight of corn. 5 parts by weight of the inoculated, cultured, fermented, dried and pulverized, and a garlic peel fermented feed using a ryzopus strain, and a method for producing the same.

Description

Garlic skin fermented feeds using Rizofus strains and methods for manufacturing the same}

The present invention relates to a garlic peel fermented feed using a ryzopus strain and a method for producing the same.

In general, the most problematic part when raising large amounts of livestock is the problem of intensive breeding and various bacterial or viral diseases according to the environment. However, it is true that until now, as a measure for disease, it has only relied on treatment or prevention by antibiotics.

However, the dependence on antibiotics as described above increases the cost burden on livestock farmers due to an increase in production costs, and has side effects that cause safety problems due to the residual antibiotics to livestock consumers.

In the case of blended feed, about 23 antibiotics are blended. However, consumer perceptions of these antibiotics are rapidly changing.

The trend of antibiotic abuse stems from the breeding environment of livestock. In other words, the majority of livestock farms are rearing excessively in order to create high added value, which is a direct cause of weakening the breeding environment due to problems such as ventilation. Specifically, ventilation of the feedlot is mostly performed through a ventilator, but the complete circulation of the gas discharged from the feedlot is difficult due to an increase in expensive equipment installation costs and heating costs.

However, this lack of ventilation causes stress in livestock and frequently causes respiratory diseases. In particular, in the case of overcrowded livestock, immunity is weakened due to stress, etc., and is easily exposed to diseases caused by pathogenic microorganisms such as E. coli, Salmonella, Stephylococcus, Enterococcus, as well as respiratory diseases. Is known.

Therefore, there is a strong tendency for livestock farms to choose a method of increasing the amount of antibiotics used as the easiest way to cope with the above problems, and this tendency further strengthens dependence on antibiotics.

On the other hand, with the development of eating out culture, consumers' preferences for food are gradually changing to a trend toward preferring safe and clean organic foods. Therefore, it can be said that the breeding of livestock dependent on antibiotics is a direction that goes against the above trend. Therefore, a countermeasure capable of effectively reducing the dependence on antibiotics as described above is required.

Republic of Korea Patent Publication No. 10-2011-0130565

The present inventors have made diligent efforts to solve the problems of the prior art as described above, select natural ingredients that can effectively strengthen antibacterial activity against pathogens and immunity against diseases, and inoculate strains capable of strengthening antibacterial activity and immunity. In the case of fermentation, it was found that the antibacterial properties and immune properties of feed can be greatly improved, and the present invention was completed.

Therefore, an object of the present invention is to provide a garlic peel fermented feed using a Rhizopus strain having significantly improved antibacterial and immune properties, and a method for producing the same.

The present invention, in order to achieve the above object,

0.1 to 5 parts by weight of a rhizopus strain in 100 parts by weight of a mixture containing 10 to 40% by weight of garlic peel, 10 to 30% by weight of rice bran residue, 10 to 30% by weight of soybean residue, and 20 to 60% by weight of corn After inoculation, culture and fermentation, it provides a garlic peel fermentation feed using a ryzopus strain, characterized in that the dried and pulverized.

The mixture is a plant hot water extract of 3 to 10% by weight of a mixture containing Boswellia ceretta, gazania, and Silene villosa in a weight ratio of 1 to 3: 1 to 3: 1 to 3 It may further include.

The Rhizopus strain may have an accession number of KCTC10597BP, which is pre-sale from the Institute of Biotechnology Gene Bank (KCTC).

In addition, the present invention

(a) preparing a mixture comprising 10 to 40% by weight of garlic peel, 10 to 30% by weight of rice bran residue, 10 to 30% by weight of soybean residue, and 20 to 60% by weight of corn;

(b) inoculating 0.1 to 5 parts by weight of a Rhizopus strain to 100 parts by weight of the mixture, and cultivating fermentation for 1 to 15 days; And

(c) drying and pulverizing the fermented product prepared in step (b) above; it provides a method for producing a garlic peel fermented feed using a lysopus strain comprising.

The mixture of step (a) is a plant hot-water extract of a mixture containing Boswellia ceretta, gazania, and Silene villosa in a weight ratio of 1 to 3: 1 to 3: 1 to 3 It may further contain 3 to 10% by weight.

The Rhizopus strain of step (b) may have a deposit number of KCTC10597BP, which has been pre-ordered from the Institute of Biotechnology Gene Bank (KCTC).

The garlic peel fermented feed using the Rhizopus strain of the present invention is natural ingredients that can effectively enhance antibacterial properties and immune properties. Garlic peel, rice bran residue, soybeans, and corn are mixed in a specific ratio, and the mixture has antibacterial properties and As a strain capable of enhancing immunity, it is prepared by inoculating and fermenting a Rhizopus strain, thereby providing an effect of greatly improving the antibacterial and immune properties of feed.

In particular, the feed of the present invention including a specific hot water extract as a component that can greatly enhance the antibacterial and immune properties of the feed provides more excellent antibacterial properties and immune properties.

In addition, the method for producing a garlic peel fermented feed using the Rhizopus strain of the present invention provides a method for efficiently producing a feed that provides the excellent effects as described above.

Hereinafter, the present invention will be described with reference to preferred embodiments.

The present invention is from 0.1 to 100 parts by weight of a mixture containing 10 to 40% by weight of garlic peel, 10 to 30% by weight of rice bran residue, 10 to 30% by weight of soybean residue, and 20 to 60% by weight of corn. It relates to a garlic peel fermentation feed using a ryzopus strain, characterized in that 5 parts by weight of inoculation, culture and fermentation, followed by drying and pulverization.

The garlic peel fermented feed using the Rhizopus strain of the present invention provides the effect of enhancing the antibacterial properties of the feed against pathogenic bacteria and the immune properties against diseases.

In addition, the present invention is to further strengthen the antibacterial properties of the feed against pathogenic bacteria and the immune properties against diseases, Boswellia Sereta, Gazania, and Seaside Silene (Silene villosa) 1 to 3: 1 ~3: It may further contain 3 to 10% by weight of the plant hot water extract of the mixture containing in a weight ratio of 1 to 3.

As the Rhizopus strain, KCTC10597BP may be preferably used as an accession number pre-ordered from the Institute of Biotechnology Gene Bank (KCTC).

The garlic peel is a by-product discarded as a garlic by-product, and has been reported to contain at least 10 times more physiologically active substances than the edible part of garlic. Garlic contains ingredients such as allicin, ajoene, and s-allyl cysteine, and these have anti-cancer properties, anti-oxidation, immunity, antibacterial, anti-cholesterol, etc. Is known. Therefore, it can be seen that the garlic peel used in the present invention contains more of the above ingredients than the garlic edible part.

In particular, considering that the garlic peel is hardly used and discarded, the garlic peel can be said to be an optimized material as a feed for enhancing antibacterial and antibiotic effects in the feed.

The rice bran residue means the residue remaining after the oil is squeezed out of rice bran.

Rice bran is separated when rice is milled, and refers to the portion of brown rice that includes rice stalks and the outer part excluding white rice. As a nutrient source, rice bran is known to have various physiological effects, including cholesterol-inhibiting effects, as it contains various active ingredients such as unsaturated fatty acids, proteins, carbohydrates, vitamin E, dietary fiber, and orizanol. Even after the oil is squeezed, since a large amount of beneficial ingredients for livestock remain in the dregs, such rice bran dregs are used as feed ingredients in the present invention.

The soybean residue refers to a component remaining after oil is squeezed from soybeans. Soybeans contain a lot of protein (about 40%) and lipids (about 25%), and the fatty acid composition of lipids contains about 50% of linoleic acid (C18:2), which has the effect of lowering blood cholesterol, and α-linolenic acid (C18). It contains 7-10% of :3). In addition, soybeans contain phenolic compounds such as dietary fiber, oligosaccharides, phytic acid, soy protein and its peptides, vegetable sterols, especially isoflavones. The isoflavones daidzin and genistin and their aglycones are known to be effective in preventing blood cholesterol reduction, arteriosclerosis, menopausal syndrome, osteoporosis, breast cancer, and prostate cancer. Such physiological activity of soybeans is reported to be stronger in aglycone forms such as genistein and daidzein rather than glycoside forms.

Therefore, since soybean residue contains a large amount of the above beneficial ingredients, soybean residue provides a very beneficial effect on the growth of livestock.

The Boswellia Sereta used for the production of the plant hot water extract grows naturally in Africa, southern Arabia, or India, and a pale greenish essence flows out when the stem is wounded. This essence has traditionally been used for inflammation such as skin rashes and ulcers and respiratory diseases such as asthma, bronchitis and laryngitis, and is known to have medical effects on arthritis. The Boswellia serrata is a representative plant of the genus Boswellia serrata, and its extract contains a large amount of boswellic acid, thus exhibiting very excellent inhibitory effect on elastase activity and inhibition of glycosaminoglycan degradation.

The gazania used for the production of the plant hot water extract is a perennial plant in the family Asteraceae, and the origin is Southern Africa. Gazania has been reported to have hepatoprotective and antioxidant activities (International Journal of Pharmacognosy and Phytochemical Research 2016; 8(7); 1121-1131).

The seaside sirene (Silene villosa), which is used for the production of the plant hot water extract, is a desert plant and is known to grow on sandy beaches along the Mediterranean Sea. It has been reported to have an effect on heart and kidney dysfunction (International Journal of Pharmacology, 2018, Volume: 14, Issue: 7, Page No.: 1001-1009).

In the present invention, all of the flowers, stems, and leaves of seaside siren may be used.

The inventors of the present invention were studying components that can further strengthen the antibacterial activity against pathogenic bacteria and the immunity against diseases of the feed. Hot water from a mixture containing the above three plants in a weight ratio of 1 to 3: 1 to 3: 1 to 3 In the case of extraction, it was found that excellent effects can be obtained.

The feed of the present invention can be used for the growth of various livestock such as chickens, ducks, dogs, pigs, and cows. In particular, it can be used alone or in a form added to general feed. When mixed with general feed, it may be added in an amount of 0.1 to 10% by weight based on 100 parts by weight of feed.

In addition, the present invention

(a) preparing a mixture comprising 10 to 40% by weight of garlic peel, 10 to 30% by weight of rice bran residue, 10 to 30% by weight of soybean residue, and 20 to 60% by weight of corn;

(b) inoculating 0.1 to 5 parts by weight of a Rhizopus strain to 100 parts by weight of the mixture, and cultivating fermentation for 1 to 15 days; And

(c) drying and pulverizing the fermented product prepared in step (b) above; it provides a method for producing a garlic peel fermented feed using a lysopus strain comprising.

As the Rhizopus strain, KCTC10597BP may be preferably used as an accession number pre-ordered from the Institute of Biotechnology Gene Bank (KCTC).

In the mixture of step (a), in order to strengthen the antibacterial properties against pathogens and the immune properties against diseases, Boswellia Sereta, Gazania, and Silene villosa are added 1~3:1~ 3: 3 to 10% by weight of the hot-water plant extract of the mixture containing in a weight ratio of 1 to 3 may be further included.

As the Rhizopus strain of step (b), KCTC10597BP may be preferably used as the deposit number obtained from the Gene Bank of Biotechnology Research Institute (KCTC).

In the step (a), each component may be pulverized in advance to form a mixture. In addition, since the pulverization process is performed in step (c) later, the mixture may be formed without pulverization.

In the step (b), the culture fermentation may be carried out at 10 to 40° C., and it may be convenient to carry out at room temperature.

The drying method in step (c) is not particularly limited, and may be performed by a method known in the art.

After the pulverization in step (c), the form of the feed may be in the form of powder, granules, or pellets, but the form is not particularly limited.

In the step (a), the garlic peel or mixture may be steamed for 0.3 to 3 hours at 100 to 180° C., and the water may be filtered to produce sludge. In the above, moisture may be removed by various methods known in the art, and for example, a strainer may be used. The mixture may be used in a state containing about 5 to 40% by weight of moisture.

Hereinafter, the present invention will be described in more detail through examples. However, the following examples are for explaining the present invention more specifically, and the scope of the present invention is not limited by the following examples. The following examples can be appropriately modified and changed by those skilled in the art within the scope of the present invention.

Preparation Example 1: Preparation of raw bacteria

The Rhizopus spp, with the deposit number KCTC 10597BP, was sold from the Gene Bank of Biotechnology Research Institute (KCTC) located at 52 Eoeun-dong, Yuseong-gu, Daejeon. Since pre-sale spawns have weak microbial power, they were subcultured to increase the microbial power, and then transplanted and cultured in a medium.

Preparation Example 2: Preparation of plant hot water extract

When 300 parts by weight of water is added to 100 parts by weight of a mixture of dried Boswellia, gazania and Silene villosa in a weight ratio of 1:1:1, and the volume becomes 1/2 Boil to extract the active ingredient to prepare a plant hot water extract.

Example 1: Preparation of Garlic Peel Fermented Feed Using Rhizopus Strains

Based on the total weight of the mixture, 20% by weight of garlic peel, 20% by weight of rice bran residue, 20% by weight of soybean residue, and 40% by weight of corn were mixed. 0.2 parts by weight of a Rhizopus strain was inoculated to 100 parts by weight of the mixture, and cultured and fermented at room temperature for 5 days. The fermented product was dried at 45° C. for 14 hours and then pulverized into granules to prepare feed.

The garlic peel was steamed at 150° C. for 1 hour, and then the water was filtered through a strainer and used.

Example 2: Preparation of Garlic Peel Fermented Feed Using Rhizopus Strains

Based on the total weight of the mixture, 20% by weight of garlic peel, 20% by weight of rice bran residue, 20% by weight of soybean residue, 35% by weight of corn, and 5% by weight of the plant hot water extract prepared in Preparation Example 2 were mixed. 0.2 parts by weight of a Rhizopus strain was inoculated to 100 parts by weight of the mixture, and cultured and fermented at room temperature for 5 days. The fermented product was dried at 45° C. for 14 hours and then pulverized into granules to prepare feed.

The garlic peel was steamed at 150° C. for 1 hour, and then the water was filtered through a strainer and used.

Test Example 1: Safety test of the plant hot water extract of Preparation Example 2

In order to test the safety of the hot water extract prepared in Preparation Example 2, 10 parts by weight of the hot water extract was mixed with 100 parts by weight of the broiler feed, and 100 broilers were bred for 30 days to observe the changes in the broiler chickens. As a control, 100 broilers fed the feed without mixing the hot water extract was used. The test results were as follows.

Broiler dead head The number of inventions of the disease Test group (feeding mixed feed with hot water extract) 2 One Control group (feed only) 3 3

As can be seen from Table 1, the hot water extract of Preparation Example 2 was not only safe, but was also found to be more beneficial to the health of broilers.

Test Example 2: Antibacterial activity test of the plant hot water extract of Preparation Example 2

The plant hot-water extract of Preparation Example 2 was added to the culture medium (hereinafter, referred to as BHI Broth) prepared in advance using the experimental section (additional section), and the results were observed after mixed culture with pathogenic microorganisms.

As a culture medium, BHI (brain heart infusion; Calf Brain infusion from 200g, Beef Heart infusion from 250g, Bacto Protearse Peptone 10g, Bacto Dextrose 2g, Sodium Chloride 5g, Disodium Phosphate 2.5g / 1 liter) was used as a culture medium. Dogs (Flask 100ml) and 4 controls (Flask 100ml) were prepared, and then sterilized at 120°C for 15 minutes and cooled to 45°C.

Escherichia coli (ATCC 11775), Salmonella typhimurium (ATCC 12023), Staphylcoccus aureus (KCTC 1621), Enterococcus faecalis (KCTC 3206) were inoculated with 0.05% (v/v) to each experimental and control group. Thereafter, the plant hot water extract of Preparation Example 2 was added to the experimental section (additive) at a level of 0.2% (v/v), and cultured at 37° C. for 24 hours and then observed.

The determination was done by smearing (spreading a certain amount of liquid evenly on the medium). To explain in more detail, after incubation, 1 ml of the sample was collected from each experimental and control flask, diluted 10 times in 9 ml of sterilized water, and repeated 10 times sequentially. The dilution ratio of the sample was adjusted according to the method. Samples diluted here were inoculated into BHI agar prepared at 0.1 ml each, spread evenly with a triangular glass rod, incubated at 37° C. for 24 hours, and then the number of colonies generated was determined by colonizing. The analysis results are shown in Table 2 below.

Added strain Treatment Result (cfu/ml) Escherichia coli
(ATCC 11775) No additives 1.2 × 10^9 0.2% added 1.5 × 10^1 Salmonella typhimurium
(ATCC 12023) No additives 1.4 × 10^7 0.2% added 2.1 × 10^1 Staphylococcus aureus
(KCTC 1621) No additives 1.4 × 10^8 0.2% added Not detected Enterococcus faecalis
(KCTC 3206) No additives 1.1 × 10^9 0.2% added Not detected

As can be seen in Table 1, in the case of the addition of the hot water extract of the plant of Preparation Example 2, the killing effect of 99.99% or more was exhibited against pathogenic microorganisms.

Test Example 3: Analysis of the components of the feed produced in Example 1

The ingredients of the feed prepared in Example 1 were analyzed by requesting the Korea Feed Association Feed Technology Research Institute. As a result, the composition and content were confirmed as follows.

Test result Test method moisture (%) 22.30 Feed standard analysis 1.1 A. 135 degrees 2 hours Crude protein (%) 26.18 Feed Standard Analysis 1.2.B. Kjeltec Method Crude fat (A) (%) 16.56 Feed standard analysis 1.4.D. Filter bag analysis method Crude fiber (F) (%) 9.56 Feed Standard Analysis 1.3. Crude Fiber Viewed (%) 7.40 Feed standard analysis 1.5. calcium (%) 0.49 Feed standard analysis 4.19. ICP emission spectroscopy method sign (%) 1.29 Feed standard analysis 4.19. ICP emission spectroscopy method Magnesium (%) 0.49 Feed standard analysis 4.19. ICP emission spectroscopy method Zinc (ppm) 71 Feed standard analysis 4.19. ICP emission spectroscopy method Salinity (%) 1.72 Feed standard analysis 4.8.A. Appropriate method

Test Example 4: Evaluation of the effect of the feed prepared in Example 1 on the growth of pathogenic microorganisms

0.2% (w/v) of the feed produced in Examples 1 and 2 was added to the culture medium (hereinafter referred to as BHI Broth) prepared in advance using the experimental section (additive), and the results were observed after mixed culture with pathogenic microorganisms. .

As a culture medium, BHI (brain heart infusion; Calf Brain infusion from 200g, Beef Heart infusion from 250g, Bacto Protearse Peptone 10g, Bacto Dextrose 2g, Sodium Chloride 5g, Disodium Phosphate 2.5g / 1 liter) was used as a culture medium. After preparing dogs (Flask 100ml) and 4 controls (Flask 100ml), sterilized at 121°C for 15 minutes was cooled to 45°C.

Escherichia coli (ATCC 11775), Salmonella typhimurium (ATCC 12023), Staphylcoccus aureus (KCTC 1621), Enterococcus faecalis (KCTC 3206) were inoculated with 0.05% (v/v) to each experimental and control group, and then the Example 1 And the feed prepared in 2 was added to the experimental group (additive) at a level of 0.2% (w/v) and observed after culturing at 37° C. for 24 hours.

The determination was done by smearing (spreading a certain amount of liquid evenly on the medium). To explain in more detail, after incubation, 1 ml of the sample was collected from each experimental and control flask, diluted 10 times in 9 ml of sterilized water, and repeated 10 times sequentially. The dilution ratio of the sample was adjusted according to the method. Samples diluted here were inoculated into BHI agar prepared at 0.1 ml each, spread evenly with a triangular glass rod, incubated at 37° C. for 24 hours, and then the number of colonies generated was determined by colonizing.

The analysis results are shown in Table 4 below.

Added strain Experiment Result (cfu/ml) Escherichia coli
(ATCC 11775) Comparative example No additives 1.3 × 10^9 Example 1 feed 0.2% added 2.9 × 10^2 Example 2 feed 0.2% added 1.9 × 10^2 Salmonella typhimurium
(ATCC 12023) Comparative example No additives 1.5 × 10^7 Example 1 feed 0.2% added 1.5 × 10^2 Example 2 feed 0.2% added 1.0 × 10^2 Staphylococcus aureus
(KCTC 1621) Comparative example No additives 1.6 × 10^8 Example 1 feed 0.2% added Not detected Example 2 feed 0.2% added Not detected Enterococcus faecalis
(KCTC 3206) Comparative example No additives 1.3 × 10^9 Example 1 feed 0.2% added Not detected Example 2 feed 0.2% added Not detected

As can be seen in Table 4, in the case of the feed addition tool prepared according to the present invention, it exhibited a killing effect of 99% or more with respect to pathogenic microorganisms. The above effect seems to be due to the antibacterial action of the substances added to the feed prepared by the present invention. Therefore, in the case of using the feed of the present invention, it can be seen that it is possible to protect livestock from Salmonella, E. coli, and food poisoning bacteria without separately feeding antibiotics.

Test Example 5: Comparative evaluation of the effect of the feed prepared in Example 1 on the growth of pathogenic microorganisms

The results were determined through a sensitivity test using the feed prepared in Examples 1 and 2 and antibiotics (Ampicillin, Tetracycline, Polymixin B, Penicillin). This is the pathogenic microorganisms E. coli, Salmonella, Stephylococcus bacteria, Enterococcus bacteria in the prepared BHI agar medium prepared before hardening after sterilizing at 121℃ for 15 minutes and then cooling at about 45℃ when manufacturing BHI agar. The prepared feed, that is, 99 ml of distilled water was added to 1 g of the prepared feed, that is, to a paper disc (diameter 8 mm), after inoculating evenly on the agar medium with a previously sterilized cotton swab. Dispense 50 microns (µl) of each sample diluted 100 times in the same way using a pipette and dry well. Then, after attaching to the center of the agar medium, it was turned over and observed by culturing at 37° C. for 24 hours, and then the circle (diameter mm) formed was observed and determined.

The analysis results are shown in Table 5 below.

Added strain Antibiotic administration group Example 1
Feed administration group Example 2
Feed administration group Escherichia coli
(ATCC 11775) Ampicillin: 37 mm 15 mm 19 mm Tetracycline: 20 mm Polymixin B: 13 mm Penicillin: 14 mm Salmonella typhimurium
(ATCC 12023) Ampicillin: 22 mm 15 mm 18 mm Tetracycline: 18 mm Polymixin B: 13 mm Penicillin: 22mm Staphylococcus aureus
(KCTC 1621) Ampicillin: 49 mm 15 mm 22 mm Tetracycline: 27 mm Polymixin B: 13 mm Penicillin: 53 mm Enterococcus faecalis
(KCTC 3206) Ampicillin: 30 mm 16 mm 20 mm Tetracycline: 23 mm Polymixin B: 14 mm Penicillin: 25mm

As can be seen in Table 5, from the test group treated with the feeds 1 and 2 in the practice of the present invention for pathogenic microorganisms and the comparison group treated with general antibiotics, the test group treated with the feed of the present invention is a whole. It can be seen that it showed an excellent antibacterial effect as well.

Test Example 6: Comparison of cultivation and death of broiler chickens

The feed prepared in Examples 1 and 2 was divided into 3 test zones based on 3 dongs in the flock out of 30,000 number of breeding numbers, and a test dong in which 2% by weight of the feed produced in Examples 1 and 2 was added each 10,000 numbers. It consisted of a control building and the experiment was conducted for 30 days. The test results are shown in Table 6 below.

Daejo-dong
(General feed provided) Test Building 1
(Example 1 feed 2% by weight mixing provided) Test Building 2
(Example 2 feed 2% by weight mixing provided) Remark Harvest 10,000 10,000 10,000 - Number of harvests 10,300 10,300 10,300 Breeding in excess of 3% Selection and death 325 262 219 19-33% reduction Number of shipments 9,975 10,038 10,081

As can be seen in Table 6, in Test Building 1, which was provided by mixing the feed of Example 1 of the present invention at 2% by weight compared to the control copper, culling and mortality were reduced by 19%, and the feed of Example 2 was reduced by 2% by weight. It can be seen that in Test Building 2 provided by mixing in %, selection and death were reduced by 33%. These results show that the feed prepared in Examples 1 and 2 of the present invention has antibacterial activity against pathogens and immunity against disease in broiler growth. It indicates that you have strengthened.

Claims (7)

10-40% by weight of garlic peel; 10-30% by weight of rice bran residue; 10-30% by weight of soybean residue; 20-60% by weight of corn; And Boswellia Sereta, Gazania (gazania), and seaside sirene (Silene villosa) 1-3: 1-3: 3 to 10% by weight of a plant hot water extract of a mixture comprising a weight ratio of 1 to 3; Garlic skin fermentation feed using a ryzopus strain, characterized in that prepared by a manufacturing method of inoculating 0.1 to 5 parts by weight of a ryzopus strain, followed by culture and fermentation, and then drying and pulverizing 100 parts by weight of a mixture containing 100 parts by weight. delete The method of claim 1,
The Rhizopus strain is a garlic peel fermented feed using a Rhizopus strain, characterized in that the deposit number received from the Biotechnology Research Institute Gene Bank (KCTC) is KCTC10597BP. (a) preparing a mixture comprising 10 to 40% by weight of garlic peel, 10 to 30% by weight of rice bran residue, 10 to 30% by weight of soybean residue, and 20 to 60% by weight of corn;
(b) inoculating 0.1 to 5 parts by weight of a Rhizopus strain to 100 parts by weight of the mixture, and cultivating fermentation for 1 to 15 days; And
(c) drying and pulverizing the fermented product prepared in step (b); including,
The mixture of step (a) is a plant hot-water extract of a mixture containing Boswellia ceretta, gazania, and Silene villosa in a weight ratio of 1 to 3: 1 to 3: 1 to 3 3 to 10% by weight; A method for producing a garlic peel fermented feed using a ryzopus strain characterized in that it further comprises. delete The method of claim 4,
The method for producing a garlic peel fermented feed using a ryzopus strain, characterized in that the deposit number received from the biotechnology research institute gene bank (KCTC) is KCTC10597BP.
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