KR102236971B1 - Color-based anemia self-testing device and method using period blood - Google Patents

Abstract

The present invention relates to a device and method for self-diagnosis of color-based anemia using menstrual blood. More specifically, the present invention relates to the device and method for self-diagnosis of color-based anemia using menstrual blood capable of checking the presence of anemia by visually judging color according to the level of hemoglobin using menstrual blood through a device collecting only an absolute amount of blood without using a complex hemoglobin measurement algorithm and device.

Description

Color-based anemia self-testing device and method using period blood}

The present invention relates to an apparatus and method for self-diagnosing anemia based on color using menstrual blood, and more specifically, the level of hemoglobin using menstrual blood through a device that collects only absolute blood without using a complex hemoglobin measurement algorithm and device. The present invention relates to a color-based anemia self-diagnosis apparatus and method using menstrual blood, capable of confirming the presence or absence of anemia by visually determining the color according to the color.

Hemoglobin is an iron-containing protein present in red blood cells and is the main transporter of oxygen in the blood. Hemoglobin deficiency leads to symptoms of anemia. Anemia has a wide variety of causes, and measurement of hemoglobin concentration is used to classify anemia by cause. For example, in the case of anemia caused by iron deficiency, these red blood cell indices decrease, and _{in the case of anemia caused by vitamin B 12} deficiency or folic acid deficiency, it increases. Anemia can occur due to a number of other causes. Since the erythrocyte indexes are different for each diagnosis, it is very important to measure the amount of hemoglobin in the differential diagnosis of anemia for the first time. In addition, hemoglobin content is a sensitive indicator of iron deficiency, and these indicators are used to diagnose diseases caused by iron deficiency or to monitor the efficacy of intravenous iron therapy.

In this regard, as a device for diagnosing anemia by measuring the concentration of hemoglobin, “a disposable current detection method for simultaneous measurement of hemoglobin and glycated hemoglobin” of Korean Patent Registration No. 10-1818368 has been proposed.

According to the “Disposable Current Detection Method for Simultaneous Measurement of Hemoglobin and Glycosylated Hemoglobin” in Korean Patent Registration No. 10-1818368, a current method was used instead of a measurement method such as high performance liquid chromatography (HPLC) or impedance method that has been used. A method of detecting a microfluidic type for simultaneous detection without going through the separation step of glycosylated hemoglobin and hemoglobin using a gold nanoparticle layer and a conductive polymer composite layer has been disclosed.

In addition, Korean Patent Registration No. 10-1531158 has proposed "a hemoglobin concentration measuring device and its method using photothermal effect".

According to the Republic of Korea Patent Registration No. 10-1531158, "a hemoglobin concentration measuring device and its method using photothermal effect", a reference light source, a photothermal light source, a cuvette part for receiving a collected blood sample, and a reference beam emitted from the reference light source part are The phase of the interference signal, which is incident on the cuvette and reflected from the top and bottom of the cuvette, changes after the light-heating beam enters the cuvette from the light-heating light source, and hemoglobin absorbs it and releases heat to generate the light-heating effect. It is a hemoglobin concentration measuring device composed of a concentration calculation unit that calculates the concentration of hemoglobin by using.

However, in general, blood collection is essential to measure the concentration of hemoglobin in the related art, and the device and method are designed to be judged by an expert.

Therefore, there is a need for an apparatus and method capable of quickly and simply measuring the concentration of hemoglobin.

Korean Patent Registration No. 10-1818368 Korean Patent Registration No. 10-1531158

The present invention is to solve such a conventional problem, and to provide a color-based anemia self-diagnosis apparatus and method using menstrual blood capable of quickly and simply measuring the concentration of hemoglobin.

To achieve the above object,

In one embodiment, the present invention is a color-based anemia self-diagnosis device using menstrual blood,

Menstrual blood collection means fixed to the sanitary napkin to collect a fixed amount of menstrual blood; And

When in contact with the collection means, it includes a discoloration means that changes color according to the amount of hemoglobin of the menstrual blood collected by the regular collection means,

The menstrual blood collection means includes an absorption pad through which menstrual blood of the fixed amount or less is absorbed; And

It includes a mesh-type network that does not contact the absorption pad with menstrual blood coming out of the absorption pad in excess of the fixed amount,

It provides a color-based anemia self-diagnosis apparatus using menstrual blood, characterized in that menstrual blood exceeding the fixed amount absorbed by the absorption pad may be coagulated after exiting from the mesh of the net.

The discoloration means according to an embodiment of the present invention is a mixed solution of the hydrogen peroxide and 3,3′,5,5′-tetramethylbenzidine (TMB), and the absorption pad is immersed in the mixed solution and then the It is characterized in that it discolors according to the amount of hemoglobin contained in the fixed amount.

In addition, the amount of hemoglobin is characterized in that the amount of hemoglobin in the whole menstrual blood of the fixed amount absorbed by the absorption pad.

The discoloration means may discolor the amount of hemoglobin in the whole menstrual blood of the fixed amount based on 10 g/dL.

The collecting means according to an embodiment of the present invention is characterized in that it is detachable to the sanitary napkin.

In addition, the present invention uses the color-based anemia self-diagnosis device using the menstrual blood, to contact the discoloration means with menstrual blood less than a fixed amount absorbed by the absorption pad, and diagnose the presence or absence of anemia according to the degree of discoloration of the discoloration means. It provides a color-based anemia self-diagnosis method using menstrual blood, characterized in that.

Specifically, the discoloration means is a mixed solution of hydrogen peroxide and 3,3′,5,5′-tetramethylbenzidine (TMB), and the absorption pad is immersed in the mixed solution and then contained in the fixed amount. It is characterized by discoloration depending on the amount of hemoglobin.

The discoloration means may discolor the amount of hemoglobin in the whole menstrual blood of the fixed amount based on 10 g/dL.

The color-based anemia self-diagnosis device using menstrual blood according to an embodiment of the present invention uses an absorption pad for collecting an absolute amount of menstrual blood, and measures anemia in a manner that discolors according to the amount of reference hemoglobin calculated based on the absolute amount. I can.

Therefore, unlike a method of using an optical or electrical method or calculating an absolute amount of hemoglobin in a relative amount of blood, anemia can be measured in a very intuitive and simple method.

That is, in the present invention, the presence or absence of anemia through the hemoglobin level can be easily determined by contacting the mixture solution of 3,3',5,5'-Tetramethylbenzidine (TMB) with pyral hydrogen peroxide absorbed in a fixed amount on an absorption pad attached to a sanitary napkin. Since it can be compared visually, the degree of anemia can be easily grasped. Since menstrual blood is used, blood collection is not required, and this method through a simple and inexpensive color change can be a field diagnosis tool for anemia in a limited resource environment.

1 is a block diagram of a color-based anemia self-diagnosis apparatus using menstrual blood according to an embodiment of the present invention.
2 is a schematic diagram of a color-based anemia self-diagnosis apparatus using menstrual blood according to an embodiment of the present invention (a) front, (b) back).
3 is an exploded perspective view of a color-based anemia self-diagnosis apparatus using menstrual blood according to an embodiment of the present invention.
4 is a diagram showing a mechanism of color change of TMB by hemoglobin.
5 is a flow chart showing steps of a color-based anemia self-diagnosis method using menstrual blood according to an embodiment of the present invention.
6 is a diagram illustrating a process of diagnosing the presence or absence of anemia using a discoloration means according to an embodiment of the present invention. Specifically, FIG. 6(a) shows the color of the mixed solution when the value of hemoglobin is 10 g/dL or less, and FIG. 6(b) shows the color of the mixed solution when the level of hemoglobin exceeds 10 g/dL.
7 is a photograph showing the results of reacting blood samples having hemoglobin levels of 6.6, 9.3, 10.2, 11.6, and 14.3 g/dL measured through a blood analyzer with a mixed solution of TMB/hydrogen peroxide.

In the present invention, various modifications may be made and various embodiments may be provided, and specific embodiments will be illustrated in the drawings and described in detail in the detailed description.

However, this is not intended to limit the present invention to a specific embodiment, it should be understood to include all changes, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the present invention, when it is determined that a detailed description of a related known technology may obscure the subject matter of the present invention, a detailed description thereof will be omitted.

The terms used in the present application are only used to describe specific embodiments, and are not intended to limit the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise.

In the present invention, terms such as “comprise” or “have” are intended to designate the presence of features, numbers, steps, actions, components, parts, or combinations thereof described in the specification, but one or more other features. It is to be understood that the presence or addition of elements, numbers, steps, actions, components, parts, or combinations thereof, does not preclude in advance the possibility of the presence or addition.

The present invention relates to an apparatus and method for self-diagnosing anemia based on color using menstrual blood, and more specifically, the level of hemoglobin using menstrual blood through a device that collects only absolute blood without using a complex hemoglobin measurement algorithm and device. The present invention relates to a color-based anemia self-diagnosis apparatus and method using menstrual blood, capable of confirming the presence or absence of anemia by visually determining the color according to the color.

More specifically, the present invention is a color-based anemia self-diagnosis device using menstrual blood, comprising: a menstrual blood collection means for collecting a fixed amount of menstrual blood by being fixed to a sanitary napkin; And a discoloring means that changes color according to the amount of hemoglobin of menstrual blood collected by the regular collection means when contacting the collection means, wherein the menstrual blood collection means includes an absorption pad through which menstrual blood less than the fixed amount is absorbed; And a mesh-type network that does not contact the absorption pad with menstrual blood coming out of the absorption pad in excess of the fixed amount, wherein the menstrual blood in excess of the fixed amount absorbed by the absorption pad is from the mesh of the mesh. It is characterized in that it can be solidified after exiting.

Furthermore, the present invention relates to an anemia self-diagnosis method using a color-based anemia self-diagnosis device using menstrual blood, wherein menstrual blood less than a fixed amount absorbed by the absorption pad is brought into contact with a discoloration means, and according to the degree of discoloration of the discoloration means. It provides a color-based anemia self-diagnosis method using menstrual blood, characterized in that diagnosing the presence or absence of anemia.

Hereinafter, an apparatus and method for self-diagnosing anemia based on color using menstrual blood according to an embodiment of the present invention will be described in detail.

1 is a block diagram of a color-based anemia self-diagnosis apparatus using menstrual blood according to an embodiment of the present invention, and FIG. 2 is a schematic diagram of a color-based anemia self-diagnosis apparatus using menstrual blood according to an embodiment of the present invention. (a) front, (b) back), FIG. 3 is an exploded perspective view of a color-based anemia self-diagnosis device using menstrual blood according to an embodiment of the present invention, and FIG. 4 is an exploded perspective view showing a mechanism of color change of TMB by hemoglobin. 5 is a flow chart showing the steps of a color-based anemia self-diagnosis method using menstrual blood according to an embodiment of the present invention, and FIG. 6 is a flow chart showing the presence or absence of anemia using a discoloration means according to an embodiment of the present invention. A diagram showing a diagnosis process as an example, and FIG. 7 is a photograph showing the result of reacting a blood sample with a hemoglobin level of 6.6, 9.3, 10.2, 11.6, and 14.3 g/dL measured through a blood analyzer with a TMB/hydrogen peroxide mixed solution to be.

1 to 7, the color-based anemia self-diagnosis device using menstrual blood according to an embodiment of the present invention includes a menstrual blood collection means 100 and a discoloration means 200 that changes color according to the amount of hemoglobin in menstrual blood. It is composed.

First, the menstrual blood collection means 100 is fixed to the sanitary napkin 101 to collect a fixed amount of menstrual blood, and includes an absorption pad 110 and a mesh type network 120. Specifically, the mesh-type net 120 is a sanitary napkin 101 attachment type, and a predetermined space may be formed therein, and only a fixed amount of menstrual blood or less can be collected inside the mesh-type net 120 It includes an absorbent pad (110).

In particular, the absorption pad 110 is for collecting only menstrual blood less than a fixed amount, and menstrual blood exceeding the fixed amount may be coagulated after coming out of the mesh of the mesh type network 120. Alternatively, the blood clot of the menstrual blood of a woman in the menstrual cycle is caught in a strainer, and only a fixed amount of blood or less can be absorbed by the absorption pad 110. Here, the term "fixed amount" may refer to menstrual blood that can be absorbed by the absorption pad 110, and may refer to about 5 µl of blood. It is possible to sufficiently test whether the user has anemia by reacting with the discoloration means 200 with only the 5 µl volume of blood.

The mesh-type network 120 according to an embodiment of the present invention may have a diameter or one side of 1 cm or more. However, as the diameter or length of one side of the mesh-type net 120 is shorter, there is no inconvenience of wearing a sanitary napkin. For example, the mesh type mesh 120 may be 1 cm long, and the absorbent pad 110 inserted therein may have an average diameter of 6 mm.

The collecting means 100 according to an embodiment of the present invention is characterized in that it is detachable to the sanitary napkin 101, and more specifically, a thread-like connection means on one side of the collecting means 100 or one side of the absorption pad 110 The 121 may be connected to be attached to the sanitary napkin 101.

For example, the absorption pad 110 in the mesh type network 120 may be separated with a connecting means and contacted with the discoloring means 200 to diagnose anemia. A specific method for diagnosing anemia will be described later.

Next, the color change means 200 may be a mixed solution of hydrogen peroxide and 3,3',5,5'-tetramethylbenzidine (TMB). The discoloration means 200 may be discolored through a reaction between hemoglobin, hydrogen peroxide, and TMB. Specifically, hemoglobin can be detected by the color of the oxidized TMB product by using a redox reaction between TMB and hydrogen peroxide as a catalyst.

4 is a diagram showing a mechanism of color change of TMB by hemoglobin. As shown in Fig. 4, in the presence of hydrogen peroxide (oxidant) and a small amount of hemoglobin, TMB reacts to form a blue one-electron charge transfer complex. When a large amount of hemoglobin is present, the reaction is complete to form a two-electron diamine product, which is yellow. When excess hemoglobin is present, the red color of hemoglobin combines with the yellow color of Diimine, resulting in a yellow to reddish yellow color in both proportions. Thus, such a reaction can result in a mixture of hemoglobin, TMB charge transfer complex, and TMB diamine in various colors.

That is, as described above, TMB exhibits a blue color when a small amount of hemoglobin is present, and may exhibit a yellow color when a large amount of hemoglobin is present. In addition, when excessive hemoglobin is present, yellow to reddish yellow may appear.

The discoloration means of the color-based anemia self-diagnosis device using menstrual blood according to an embodiment of the present invention discolors according to the amount of hemoglobin contained in a fixed amount after immersing the absorption pad 110, and the collecting means 100 The amount of hemoglobin of the menstrual blood collected in may be the amount of hemoglobin of the whole menstrual blood of the fixed amount absorbed by the absorption pad 110.

In particular, the discoloration means may discolor the amount of hemoglobin in the whole menstrual blood of a fixed amount based on 10 g/dL. For example, when the amount of hemoglobin in the fixed amount of menstrual blood is less than 10 g/dL, the color may become blue, and when the amount of hemoglobin in the entire menstrual blood of the fixed amount exceeds 10 g/dL, it is yellow. It may be discolored into a series.

Further, the present invention relates to a color-based anemia self-diagnosis method using menstrual blood, and a color-based anemia self-diagnosis device 10 using menstrual blood is used to discolor menstrual blood less than a fixed amount absorbed by the absorption pad 110. Contacting the means 200 (S100), and diagnosing the presence or absence of anemia (S200) according to the degree of discoloration of the discoloration means 200 (see FIG. 5).

The discoloration means may be a mixed solution of hydrogen peroxide and 3,3',5,5'-tetramethylbenzidine (TMB), and the TMB and hydrogen peroxide may be added in a ratio of 1:10 to 1:5. have. In addition, the absorption pad 110 may be discolored according to the amount of hemoglobin contained in the fixed amount after being immersed in the mixed solution.

The discoloration means 200 may discolor the amount of hemoglobin in the whole menstrual blood of the fixed amount based on 10 g/dL. In addition, the TMB mixed solution, which is the discoloration means 200, may be accommodated in a transparent solution reaction unit. Further, a comparative color (standard color) that can be compared with a discolored color may be attached to the solution reaction unit.

As described above, when the value of hemoglobin is 10 g/dL or less, the color of the mixed solution changes to blue, and when it exceeds 10 g/dL, it may appear yellow. You can turn yellow. In addition, the blue and yellow colors may be attached to the solution reaction part. At this time, if the color of the discoloration means 200 is similar when compared to the blue color, which is a comparative color, anemia may be diagnosed.

6 is a diagram illustrating a process of diagnosing the presence or absence of anemia using a discoloration means according to an embodiment of the present invention. Specifically, FIG. 6(a) shows the color of the mixed solution when the value of hemoglobin is 10 g/dL or less, and FIG. 6(b) shows the color of the mixed solution when the level of hemoglobin exceeds 10 g/dL.

For example, in the color-based anemia self-diagnosis method using menstrual blood, menstrual blood less than a fixed amount absorbed by the absorption pad 110 is brought into contact with the color changing means 200, and the mixed solution is shaken well until the color turns yellow. . And, it is possible to observe the color of the discoloration means (200, mixed solution) after an average of 30 minutes.

At this time, if the value of hemoglobin is 10 g/dL or less, as shown in FIG. 6(a), the color of the mixed solution changes to blue, and when it exceeds 10 g/dL, the red color as shown in FIG. 6(b). It may appear yellowish. That is, when the collecting means 100 comes into contact with the discoloration means 200, if the color of the discoloration means 200 appears in a color close to blue light, go to the hospital to suspect anemia and receive an accurate diagnosis. have.

That is, in the present invention, the presence or absence of anemia through the hemoglobin level can be easily determined by contacting the mixture solution of 3,3',5,5'-Tetramethylbenzidine (TMB) with pyral hydrogen peroxide absorbed in a fixed amount on an absorption pad attached to a sanitary napkin. Since it can be compared visually, the degree of anemia can be easily grasped. Since menstrual blood is used, blood collection is not required, and this method through a simple and inexpensive color change can be a field diagnosis tool for anemia in a limited resource environment.

Hereinafter, the present invention will be described through the following examples. However, the examples are for explaining the present invention in detail, and the scope of the present invention is not limited to the following examples.

<Example>

A mixed solution was prepared by mixing TMB and hydrogen peroxide solution. Specifically, a mixed solution was prepared in which 450 µl of a TMB solution and 50 µl of a 0.3% hydrogen peroxide solution were mixed.

Then, the mixed solution was reacted with a filter paper in which an appropriate amount of blood is absorbed. For reference, the filter paper was reacted with a filter paper in which blood is absorbed in a volume of about 5 µl of blood. At this time, the filter paper had a size of 6 mm semicircle.

More specifically, a test was performed with blood samples having hemoglobin levels of 6.6, 9.3, 10.2, 11.6, and 14.3 g/dL measured through a conventional blood analyzer.

And, the results are shown in FIG. 7.

7 is a photograph showing the results of reacting blood samples having hemoglobin levels of 6.6, 9.3, 10.2, 11.6, and 14.3 g/dL measured through a blood analyzer with a TMB/hydrogen peroxide mixed solution.

Referring to FIG. 7, it was confirmed that the color of the mixed solution was changed according to the hemoglobin level.

10: anemia self-diagnosis device
100: collection means
101: sanitary napkin 110: absorption pad
120: mesh type network 121: connecting means
200: discoloration means

Claims (8)

As a color-based anemia self-diagnosis device using menstrual blood,
Menstrual blood collection means fixed to the sanitary napkin to collect a fixed amount of menstrual blood; And
When in contact with the collection means, it includes a discoloration means that changes color according to the amount of hemoglobin of the menstrual blood collected by the regular collection means,
The menstrual blood collection means includes an absorption pad through which menstrual blood less than the fixed amount is absorbed; And
And a mesh-type network that does not contact the absorption pad with menstrual blood coming out of the absorption pad in excess of the fixed amount,
Color-based anemia self-diagnosis device using menstrual blood, characterized in that menstrual blood exceeding the fixed amount absorbed by the absorption pad may be coagulated after exiting from the mesh of the net.
The method of claim 1,
The discoloration means is a mixed solution of hydrogen peroxide and 3,3',5,5'-tetramethylbenzidine (TMB), and the absorption pad is the amount of hemoglobin contained in the fixed amount after being immersed in the mixed solution. Color-based anemia self-diagnosis device using menstrual blood, characterized in that the color changes according to.
The method of claim 1,
The color-based anemia self-diagnosis device using menstrual blood, characterized in that the amount of hemoglobin is the amount of hemoglobin in the whole menstrual blood of the fixed amount absorbed by the absorption pad.
The method of claim 3,
The discoloration means is a color-based anemia self-diagnosis device using menstrual blood, characterized in that the amount of hemoglobin in the whole of the fixed amount of menstrual blood is discolored based on 10 g/dL.
The method of claim 1,
The collecting means is a color-based anemia self-diagnosis device using menstrual blood, characterized in that it is detachable to a sanitary napkin.
Using the color-based anemia self-diagnosis device using menstrual blood according to any one of claims 1 to 5,
Color-based anemia self-diagnosis method using menstrual blood, characterized in that the menstrual blood less than a fixed amount absorbed by the absorption pad is brought into contact with the discoloration means, and the presence or absence of anemia is diagnosed according to the degree of discoloration of the discoloration means.
The method of claim 6,
The discoloration means is a mixed solution of hydrogen peroxide and 3,3',5,5'-tetramethylbenzidine (TMB), and the absorption pad is the amount of hemoglobin contained in the fixed amount after being immersed in the mixed solution. Color-based anemia self-diagnosis method using menstrual blood, characterized in that the color changes according to.
The method of claim 6,
The discoloration means is a color-based anemia self-diagnosis method using menstrual blood, characterized in that the amount of hemoglobin in the whole of the fixed amount of menstrual blood is discolored based on 10 g/dL.

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