KR102236036B1

KR102236036B1 - Process for improving chlorella and chlorella obtained by using the same

Info

Publication number: KR102236036B1
Application number: KR1020200099597A
Authority: KR
Inventors: 정유철; 전진영
Original assignee: 대상 주식회사
Priority date: 2020-08-10
Filing date: 2020-08-10
Publication date: 2021-04-05
Also published as: KR20200100566A

Abstract

본 발명은 클로렐라에서 특정 유전자 즉, 서열번호 1 내지 6의 유전자로 이루어진 군으로부터 1종 이상 선택된 유전자의 발현을 조절하는 것을 포함하는 클로렐라의 개량방법을 제공한다. 또한, 본 발명은 상기 개량방법에 의해 얻어진 클로렐라 및 상기 유전자의 발현을 조절하는 프로모터가 삽입된 벡터를 제공한다.The present invention provides a method for improving chlorella comprising controlling the expression of a specific gene in chlorella, that is, one or more genes selected from the group consisting of the genes of SEQ ID NOs: 1 to 6. In addition, the present invention provides a vector into which a promoter that regulates the expression of the chlorella and the gene obtained by the above improvement method is inserted.

Description

Method for improving chlorella and chlorella obtained by using it {Process for improving chlorella and chlorella obtained by using the same}

본 발명은 클로렐라에서 특정 유전자의 발현을 조절하는 것을 포함하는 클로렐라의 개량방법에 관한 것이다. 또한, 본 발명은 상기 개량방법에 의해 얻어진 클로렐라 및 상기 유전자의 발현을 조절하는 프로모터가 삽입된 벡터에 관한 것이다.The present invention relates to a method for improving chlorella, comprising controlling the expression of a specific gene in chlorella. In addition, the present invention relates to a vector into which a promoter for controlling the expression of the chlorella and the gene is inserted, obtained by the above improved method.

클로렐라는 지구나이와 비슷하며 식물 중 가장 오래된 단세포 녹조식물 중 하나로 알려져 있다. 광합성과 세포분열이 우수한 클로렐라는 엽록소는 물론, 단백질, 비타민, 미네랄 등 영양소가 풍부하며, 클로렐라 성장 촉진 인자인 CGF(Chlorella Growth Factor)가 함유 되어 있다. 그 밖에 클로렐라는 알칼리성 식품으로 알려져 있어 피부미용 및 항산화 작용에 도움이 되고, 면역 증진 및 배변활동에 도움이 되는 것으로 알려져 있다. 또한, 체질개선과 영양보급에 도움을 주며 성장발육에 효과가 있어, 모든 사람들이 안심하고 섭취할 수 있는 천연 식품으로 알려져 있다.Chlorella is similar to Earth's age and is known as one of the oldest single-celled green algae plants among plants. Chlorella, which is excellent in photosynthesis and cell division, is rich in nutrients such as protein, vitamins, minerals, as well as chlorophyll, and contains CGF (Chlorella Growth Factor), a chlorella growth promoting factor. In addition, since chlorella is known as an alkaline food, it is known to be helpful in skin care and antioxidant activity, and to promote immunity and bowel movements. In addition, it is known as a natural food that all people can safely consume because it helps improve constitution and supplements nutrition and is effective in growth and development.

클로렐라는 독립영양배양, 종속영양배양, 또는 이들의 조합에 의해 배양되며, 탄소원, 질소원, 미량금속, 미량원소 등을 포함하는 배지 중에서 배양될 수 있다. 또한, 연속 배양(continuous culture), 유가식 배양(fed batch culture) 등을 포함한 다양한 배양방법이 클로렐라 배양에 사용되고 있다. 예를 들어, 유가식 배양방법은 클로렐라의 생육에 필요한 에너지원(예를 들어, 탄소원)으로서 글루코오즈를 배양 배지에 직접 첨가하여 배양함으로써, 클로렐라의 빠른 성장을 유도하는 방법이다. 이러한 배양방법은 클로렐라의 성장이 빠르다는 장점이 있으나, 엽록소의 함량이 낮은 단점이 있다. 이러한 단점을 해결하기 위하여, 배지내에서 글루코오즈의 함량을 제한하는 '숙성' 공정을 도입함으로써 엽록소의 함량을 증가시키는 방법이 사용되고 있다. Chlorella is cultured by autotrophic culture, heterotrophic culture, or a combination thereof, and can be cultured in a medium containing a carbon source, a nitrogen source, a trace metal, a trace element, and the like. In addition, various culture methods including continuous culture and fed batch culture have been used for chlorella culture. For example, the fed-batch culture method is a method of inducing rapid growth of chlorella by directly adding glucose as an energy source (eg, a carbon source) required for the growth of chlorella to the culture medium and culturing it. This culture method has the advantage of rapid growth of chlorella, but has a disadvantage of low chlorophyll content. In order to solve this disadvantage, a method of increasing the content of chlorophyll by introducing a'aging' process that limits the content of glucose in the medium has been used.

따라서, 통상의 배양을 통하여 빠른 성장을 유도함과 동시에 높은 함량의 엽록소를 제공할 수 있도록 클로렐라를 개량할 경우, 숙성 공정을 수행하지 않거나 혹은 숙성 공정을 최소화할 수 있을 것으로 기대된다.Therefore, when chlorella is improved to induce rapid growth through normal culture and to provide a high content of chlorophyll, it is expected that the aging process may not be performed or the aging process can be minimized.

본 발명자들은 통상의 배양을 통하여 빠른 성장을 유도함과 동시에 높은 함량의 엽록소를 제공할 수 있도록 클로렐라를 개량하기 위하여 다양한 연구를 수행하였다. 특히, 본 발명자들은 유전자 수준의 접근을 시도하였다. 즉, 본 발명자들은 당 공급이 원할하여 세포 성장이 활발한 시점의 클로렐라와 당 공급을 제한한 뒤 엽록소가 급격이 증가되는 시점의 클로렐라를 각각 배양 과정에서 채취하여, 발현량의 차이를 나타내는 유전자를 검색하였다. 그 결과, 놀랍게도 수많은 유전자 중 특정 유전자들의 발현이 엽록소가 급격이 증가되는 시점의 클로렐라에서 현저하게 증가되거나 혹은 감소한다는 것을 발견하였다. The present inventors have conducted various studies to improve chlorella so as to induce rapid growth through normal culture and to provide a high content of chlorophyll. In particular, the present inventors attempted an approach at the gene level. That is, the present inventors limit the supply of chlorella and chlorella at the time when the cell growth is active due to the favorable supply of sugar, and then collect the chlorella at the time when the chlorophyll is rapidly increased in the culturing process, and search for genes showing the difference in expression levels. I did. As a result, surprisingly, it was found that the expression of certain genes among the numerous genes was significantly increased or decreased in chlorella at the point where chlorophyll was rapidly increased.

따라서, 본 발명은 상기 현저한 발현 증가 또는 감소를 나타낸 유전자의 발현을 조절하는 것을 포함하는 클로렐라의 개량방법을 제공하는 것을 목적으로 한다.Accordingly, an object of the present invention is to provide a method for improving chlorella, which includes controlling the expression of a gene that exhibits the remarkable increase or decrease in expression.

또한, 본 발명은 상기 개량방법에 의해 얻어진 클로렐라를 제공하는 것을 목적으로 한다.In addition, an object of the present invention is to provide chlorella obtained by the above improvement method.

또한, 본 발명은 상기 현저한 발현 증가 또는 감소를 나타낸 유전자의 발현을 조절하는 프로모터가 삽입된 벡터를 제공하는 것을 목적으로 한다.In addition, an object of the present invention is to provide a vector into which a promoter that regulates the expression of a gene that exhibits the remarkable increase or decrease in expression is inserted.

본 발명의 일 태양에 따라, 클로렐라에서 서열번호 1 내지 6의 유전자로 이루어진 군으로부터 1종 이상 선택된 유전자의 발현을 조절하는 것을 포함하는 클로렐라의 개량방법이 제공된다.According to an aspect of the present invention, there is provided a method for improving chlorella comprising controlling the expression of one or more genes selected from the group consisting of genes of SEQ ID NOs: 1 to 6 in chlorella.

일 구현예에서, 상기 조절은 클로렐라에서 서열번호 1 내지 4의 유전자로 이루어진 군으로부터 1종 이상 선택된 유전자의 발현을 증가시킴으로써 수행될 수 있다. 다른 구현예에서, 상기 조절은 클로렐라에서 서열번호 5 내지 6의 유전자로 이루어진 군으로부터 1종 이상 선택된 유전자의 발현을 억제시킴으로써 수행될 수 있다. 또한, 상기 조절은 서열번호 1 내지 6의 유전자로 이루어진 군으로부터 1종 이상 선택된 유전자의 발현을 조절하는 프로모터가 삽입된 벡터로 클로렐라를 형질전환함으로써 수행될 수 있다.In one embodiment, the regulation may be performed by increasing the expression of one or more genes selected from the group consisting of genes of SEQ ID NOs: 1 to 4 in chlorella. In another embodiment, the regulation may be performed by inhibiting the expression of one or more genes selected from the group consisting of genes of SEQ ID NOs: 5 to 6 in chlorella. In addition, the regulation may be performed by transforming chlorella with a vector into which a promoter controlling the expression of one or more selected genes from the group consisting of the genes of SEQ ID NOs: 1 to 6 is inserted.

본 발명의 다른 태양에 따라, 상기 개량방법에 의해 얻어진 클로렐라가 제공된다.According to another aspect of the present invention, there is provided chlorella obtained by the above improvement method.

본 발명의 또다른 태양에 따라, 서열번호 1 내지 6의 유전자로 이루어진 군으로부터 1종 이상 선택된 유전자의 발현을 조절하는 프로모터가 삽입된 벡터가 제공된다.According to another aspect of the present invention, there is provided a vector into which a promoter that controls the expression of one or more selected genes from the group consisting of the genes of SEQ ID NOs: 1 to 6 is inserted.

당 공급이 원할하여 세포 성장이 활발한 시점의 클로렐라와 당 공급을 제한한 뒤 엽록소가 급격이 증가되는 시점의 클로렐라에 있어서, 특정 유전자들의 발현이 엽록소가 급격이 증가되는 시점의 클로렐라에서 현저하게 증가되거나 혹은 감소한다는 것이 본 발명에 의해 밝혀졌다. 구체적으로, 서열번호 1 내지 4의 유전자의 발현이 엽록소가 급격이 증가되는 시점의 클로렐라에서 현저하게 증가되며; 또한 서열번호 5 내지 6의 유전자의 발현이 엽록소가 급격이 증가되는 시점의 클로렐라에서 현저하게 감소된다는 것이 밝혀졌다. 따라서, 본 발명의 개량방법에 따라 서열번호 1 내지 4의 유전자의 발현을 증가시켜 얻어진 클로렐라 및/또는 서열번호 5 내지 6의 유전자의 발현을 억제시켜 얻어진 클로렐라는 통상의 배양을 통하여 빠른 성장을 유도할 수 있을 뿐만 아니라, 높은 함량의 엽록소를 제공할 수 있다.In chlorella at a time when the sugar supply is adequate and cell growth is active, and in chlorella at a time when chlorophyll is rapidly increased after the supply of sugar is limited, the expression of specific genes is significantly increased in chlorella at the time when chlorophyll is rapidly increased or Or it has been found by the present invention to decrease. Specifically, the expression of the genes of SEQ ID NOs: 1 to 4 is remarkably increased in chlorella at the point in time when chlorophyll is rapidly increased; In addition, it was found that the expression of the genes of SEQ ID NOs: 5 to 6 was remarkably reduced in chlorella at the point in time when the chlorophyll rapidly increased. Therefore, according to the improved method of the present invention, chlorella obtained by increasing the expression of the genes of SEQ ID NOs: 1 to 4 and/or chlorella obtained by inhibiting the expression of the genes of SEQ ID NOs: 5 to 6 induces rapid growth through conventional culture. Not only can it, but it can provide a high content of chlorophyll.

도 1은 숙성전 및 숙성후에서 클로렐라 세포량 및 이로부터 얻어지는 엽록소 함량의 변화를 측정한 결과이다.
도 2는 숙성전 대비 숙성후에 발현량이 2배 이상 증가한 유전자를 선별한 결과를 나타낸다.
도 3은 숙성전 대비 숙성후에 발현량이 2배 이상 감소한 유전자를 선별한 결과를 나타낸다.1 is a result of measuring changes in the amount of chlorella cells and the chlorophyll content obtained therefrom before and after aging.
2 shows the results of selecting genes whose expression level is increased by 2 times or more after aging compared to before aging.
3 shows the results of selecting genes whose expression level is reduced by 2 times or more after aging compared to before aging.

본 발명은 클로렐라에서 서열번호 1 내지 6의 유전자로 이루어진 군으로부터 1종 이상 선택된 유전자의 발현을 조절하는 것을 포함하는 클로렐라의 개량방법을 제공한다.The present invention provides a method for improving chlorella comprising controlling the expression of one or more genes selected from the group consisting of the genes of SEQ ID NOs: 1 to 6 in chlorella.

본 발명에 의해, 서열번호 1 내지 4의 유전자의 발현이 엽록소가 급격이 증가되는 시점의 클로렐라에서 현저하게 증가되며; 또한 서열번호 5 내지 6의 유전자의 발현이 엽록소가 급격이 증가되는 시점의 클로렐라에서 현저하게 감소된다는 것이 밝혀졌다. 이러한 발견은, 배양 배지로부터 글루코오즈 공급이 원할할 경우, 클로렐라 세포는 광합성에 필요한 엽록소를 생산하는 생합성 기작을 억제 조절(down regulation)하고, 이를 통해 확보한 여유 리소스(ATP, 포스페이트, 이온 등)를 글루코오즈 섭취와 세포 성장에 집중하여 생활하다가, 글루코오즈의 고갈에 따라 필요한 에너지원(탄소원)을 확보하기 위하여 본연의 광합성 기작을 활성화하기 위한 생합성 기작을 활성 조절(up regulation)하여 탄소원을 확보하고자 하는 메커니즘에 기인하는 것으로 추정된다. According to the present invention, the expression of the genes of SEQ ID NOs: 1 to 4 is remarkably increased in chlorella at the point in time when chlorophyll is rapidly increased; In addition, it was found that the expression of the genes of SEQ ID NOs: 5 to 6 was remarkably reduced in chlorella at the point in time when the chlorophyll rapidly increased. These findings suggest that when a supply of glucose from the culture medium is desired, chlorella cells down-regulate the biosynthetic mechanism that produces chlorophyll required for photosynthesis, thereby securing free resources (ATP, phosphate, ions, etc.). While living by focusing on glucose intake and cell growth, a carbon source is secured by up-regulating the biosynthetic mechanism to activate the natural photosynthesis mechanism in order to secure the energy source (carbon source) required by the depletion of glucose. It is presumed to be due to the mechanism it intends to do.

상기 클로렐라는 예를 들어, 클로렐라 불가리스(Chlorella vulgaris), 클로렐라 피레노이도사(Chlorella pyrenoidosa), 클로렐라 레귤라리스(Chlorella regularis), 클로렐라 사코로필라(Chlorella saccharophila), 클로렐라 소로키니아나(Chlorella sorokiniana) 등을 제한없이 포함하며, 당업자가 입수가능한 모든 것을 포함한다. 예를 들어, 상기 클로렐라는 대한민국 특허등록 제10-0193748호에 개시된 바에 따라, 클로렐라 종균 배양을 통하여 얻어진 것을 사용할 수 있다.The chlorella, for example, Chlorella vulgaris (Chlorella vulgaris), Chlorella Pierre D'Dosa (Chlorella pyrenoidosa), Chlorella regyulra less (Chlorella regularis), pillars (Chlorella saccharophila), Chlorella Thoreau Kearney Ana (Chlorella sorokiniana) as Chlorella Sako, etc. Includes without limitation, and includes everything available to those skilled in the art. For example, as disclosed in Korean Patent Registration No. 10-0193748, the chlorella may be obtained through cultivation of chlorella spawns.

일 구현예에서, 상기 조절은 클로렐라에서 서열번호 1 내지 4의 유전자로 이루어진 군으로부터 1종 이상 선택된 유전자의 발현을 증가시킴으로써 수행될 수 있다. 다른 구현예에서, 상기 조절은 클로렐라에서 서열번호 5 내지 6의 유전자로 이루어진 군으로부터 1종 이상 선택된 유전자의 발현을 억제시킴으로써 수행될 수 있다. In one embodiment, the regulation may be performed by increasing the expression of one or more genes selected from the group consisting of genes of SEQ ID NOs: 1 to 4 in chlorella. In another embodiment, the regulation may be performed by inhibiting the expression of one or more genes selected from the group consisting of genes of SEQ ID NOs: 5 to 6 in chlorella.

상기 유전자 발현의 조절, 즉, 유전자 발현의 증가 또는 억제는 다양한 생명공학적 방법에 의해 수행될 수 있다. 예를 들어, 상기 조절은 서열번호 1 내지 6의 유전자로 이루어진 군으로부터 1종 이상 선택된 유전자의 발현을 조절하는 프로모터가 삽입된 벡터로 클로렐라를 형질전환함으로써 수행될 수 있다. 상기 벡터는 통상의 제한효소를 사용하여 프로모터를 삽입할 수 있는 벡터라면, 제한없이 사용될 수 있다. 예를 들어, 상기 벡터는 pBI121, pCAMBIA 등의 벡터를 포함하나, 이에 제한되는 것은 아니다. 상기 형질전환은 다양한 유전공학적 방법에 수행될 수 있으며, 예를 들어 일렉트로포레이션(electroporation), 아그로박테리움 매개 형질전환(Agrobacterium mediated transformation) 등에 의해 수행될 수 있다.The regulation of gene expression, ie, increase or suppression of gene expression, can be performed by various bioengineering methods. For example, the regulation may be performed by transforming chlorella with a vector into which a promoter that controls the expression of one or more selected genes from the group consisting of the genes of SEQ ID NOs: 1 to 6 is inserted. The vector may be used without limitation as long as it is a vector capable of inserting a promoter using a conventional restriction enzyme. For example, the vector includes, but is not limited to, vectors such as pBI121 and pCAMBIA. The transformation may be performed by various genetic engineering methods, for example, electroporation, Agrobacterium mediated transformation, or the like.

본 발명은 또한 상기 개량방법에 따라 얻어진 클로렐라를 제공한다.The present invention also provides chlorella obtained according to the above improved method.

본 발명은 또한 서열번호 1 내지 6의 유전자로 이루어진 군으로부터 1종 이상 선택된 유전자의 발현을 조절하는 프로모터가 삽입된 벡터를 제공한다. 상기 프로모터 및 벡터는 상기에서 설명한 바와 같다.The present invention also provides a vector into which a promoter that regulates the expression of one or more selected genes from the group consisting of the genes of SEQ ID NOs: 1 to 6 is inserted. The promoter and vector are as described above.

이하, 본 발명을 실시예를 통하여 더욱 상세히 설명한다. 그러나, 하기 실시예는 본 발명을 예시하기 위한 것으로, 본 발명이 하기 실시예에 의해 제한되는 것은 아니다.Hereinafter, the present invention will be described in more detail through examples. However, the following examples are for illustrative purposes only, and the present invention is not limited by the following examples.

실시예 Example

대한민국 특허등록 제10-0193748호에 개시된 바에 따라, 글루코오즈 공급을 통한 배양과정에서 클로렐라 세포량 및 세포내 축적된 엽록소 함량의 변화는 도 1과 같다. 도 1에서 알 수 있는 바와 같이, 글루코오즈 공급을 제한한 시점부터 엽록소의 함량은 지속적으로 증가하였다.As disclosed in Korean Patent Registration No. 10-0193748, changes in the amount of chlorella cells and the amount of chlorophyll accumulated in the cells during the culture process through glucose supply are as shown in FIG. 1. As can be seen from FIG. 1, the content of chlorophyll continued to increase from the time when glucose supply was restricted.

당 공급이 원할하여 세포 성장이 활발한 시점(즉, 배양 시작 후 30시간)의 클로렐라와 당 공급을 제한한 뒤 엽록소가 급격이 증가되는 시점(즉, 배양 시작 후 50시간(숙성 시작 후 10시간))의 클로렐라를 각각 채취하여, 그로부터 총 RNA를 분리하였다. 상기 총 RNA 분리는 QIAGEN사의 RNeasy Plant Mini Kit을 사용하여 제공된 방법에 의해 분리 및 회수함으로써 수행하였다.When the supply of chlorella and sugar is limited at the time when the supply of sugar is adequate and cell growth is active (i.e., 30 hours after the start of cultivation) and the time when the chlorophyll is rapidly increased (i.e., 50 hours after the start of cultivation (10 hours after the start of cultivation)) ) Of chlorella was collected, and total RNA was isolated therefrom. The total RNA isolation was performed by isolation and recovery by the method provided using the RNeasy Plant Mini Kit of QIAGEN.

상기 분리된 총 RNA로부터, 숙성전 대비 숙성후에 유의성 있는 유전자 발현량 차이를 나타내는 유전자(Differential Expressed Genes, DEG)를 선별하였다. 즉, 숙성전 대비 숙성후에 확인된 DEG중 증감에 따른 절대값 |2| 이상의 유전자를 선별하였으며, 그 결과 4605개의 유전자가 발현이 증가되었고(도 2 참조), 4431개의 유전자가 발현이 감소되었음을 확인하였다(도 3 참조). 도 2 및 도 3의 결과로부터, 가장 높은 발현 증가 및 감소를 나타내는 유전자는 하기 표 1과 같다.From the isolated total RNA, genes (Differential Expressed Genes, DEG) showing a significant difference in gene expression levels after maturation compared to before maturation were selected. In other words, the absolute value according to the increase or decrease in the amount of DEG checked after aging compared to before aging |2| The above genes were selected, and as a result, it was confirmed that the expression of 4605 genes was increased (see FIG. 2) and that the expression of 4431 genes was decreased (see FIG. 3). From the results of FIGS. 2 and 3, genes showing the highest expression increase and decrease are shown in Table 1 below.

서열번호Sequence number 발현Manifestation 유전자 명칭^* Gene name ^* 기원origin 1One +108.4배+108.4x PDR12 ABC transporter G family member 40PDR12 ABC transporter G family member 40 Arabidopsis thalianaArabidopsis thaliana 22 +44.8배+44.8x PDR3 ABC transporter G family member 31PDR3 ABC transporter G family member 31 Arabidopsis thalianaArabidopsis thaliana 33 +29.8배+29.8 times G4 chlorophyll synthaseG4 chlorophyll synthase Arabidopsis thalianaArabidopsis thaliana 44 +27.1배+27.1 times AT5G07020 proline-rich family proteinAT5G07020 proline-rich family protein Arabidopsis thalianaArabidopsis thaliana 55 -109.9배-109.9 times GSA2 glutamate-1-semialdehyde 2,1-aminomutase 2GSA2 glutamate-1-semialdehyde 2,1-aminomutase 2 Arabidopsis thalianaArabidopsis thaliana 66 -75.8배-75.8 times AT2G20820 hypothetical proteinAT2G20820 hypothetical protein Arabidopsis thalianaArabidopsis thaliana

*: NCBI의 데이터베이스의 유전자 몇칭*: Some names of genes in NCBI's database

상기 서열번호 1 내지 6의 유전자는 클로렐라의 엽록소 생합성 경로와 관련하여 이전에 보고된 바 없는 유전자로서 모두 애기장대(Arabidopsis thaliana)에 존재하는 것으로 알려진 유전자이다. 그럼에도 불구하고, 서열번호 1 내지 4의 유전자의 발현이 클로렐라의 숙성과정에서 현저하게 증가하고 또한 서열번호 5 및 6의 유전자의 발현이 클로렐라의 숙성과정에서 현저하게 감소한다는 것은 매우 놀라운 것이다. 따라서, 상기 유전자의 발현을 조절할 수 있는 프로모터를 삽입한 벡터로 클로렐라를 형질전환할 경우, 얻어진 클로렐라 균주는 통상의 배양을 통하여 빠른 성장을 유도할 수 있을 뿐만 아니라, 높은 함량의 엽록소를 제공할 수 있다.The genes of SEQ ID NOs: 1 to 6 are genes that have not been previously reported in relation to the chlorophyll biosynthetic pathway of chlorella and are all known to exist in Arabidopsis thaliana. Nevertheless, it is very surprising that the expression of the genes of SEQ ID NOs: 1 to 4 significantly increases during the aging process of chlorella, and that the expression of the genes of SEQ ID NOs: 5 and 6 significantly decreases during the aging process of chlorella. Therefore, when chlorella is transformed with a vector into which a promoter capable of regulating the expression of the gene is inserted, the obtained chlorella strain can induce rapid growth through conventional culture, as well as provide a high content of chlorophyll. have.

<110> DAESANG CORPORATION <120> Process for improving chlorella and chlorella obtained by using the same <130> PN0718 <160> 6 <170> KopatentIn 2.0 <210> 1 <211> 6469 <212> DNA <213> Arabidopsis thaliana <400> 1 atatcatctt tcatctacaa tttctctctt gagtttcttt tcatgtgtta aagtcttatt 60 gtcttgctaa attctttaaa tctttcatct gacacaaaca aaaaaagaga gaagaaaaaa 120 aagaagaaga ctttgatttc ttggatacaa aatggaggga actagttttc accaagcgag 180 taatagtatg agaagaaact catcggtgtg gaagaaagat tcaggaaggg agattttctc 240 gaggtcatct agagaagaag acgatgaaga agctttgaga tgggctgctc ttgagaagct 300 tcccactttt gatcgtctca ggaaaggaat cctaactgcc tcacatgccg gaggacccat 360 caacgagatc gatattcaga agcttgggtt tcaagatact aagaaactgc tagagaggct 420 catcaaagtc ggtgacgatg agcatgagaa actcctctgg aaactcaaga aacgtatcga 480 taggtataca aacttctctc tttttttttt ttagtttagc ttttaatggg aacagaggag 540 ctctgttttt gtttgatgtt catgttggag acttaagtgt taattgtatg taattatgaa 600 aatgacagag ttggaatcga tcttccgaca atagaagttc ggtttgatca tctaaaagtt 660 gaagcagagg ttcatgttgg aggcagagct ttacctacgt tcgtcaattt catctccaat 720 tttgctgatg taagaatgcg ttttttttac ttatctgttt cttgtgtcca aacaaaaact 780 agatctctaa cagtggaatt ttcgttaata ttaacagaag ttcctgaata ctctgcatct 840 tgttccgaac cgaaagaaga agttcactat actcaacgac gtcagcggaa tcgtcaagcc 900 tggcaggtga attttcagat tctgtttcac cattttaaaa tattggaaag tatttttagt 960 tatacttgca cttgttgacc catctttgaa ttctacagga tggctctgct tttgggtcct 1020 ccaagttctg ggaaaacgac cctcttgctt gccttggcgg gaaagcttga tcaagaacta 1080 aaggtaacca tctttccctc tctctatctc tgtttcaact tcatggatat gtttatatga 1140 tctgtgtgaa tcaattgtaa actttgttct ttgattttgt agcaaactgg aagagtgaca 1200 tacaatggtc atggaatgaa cgagtttgtg ccacaaagaa cagctgcata tatcggccaa 1260 aacgatgttc atatcggtga gatgactgtt cgtgagactt ttgcttacgc agctcgcttc 1320 caaggtgttg gttcgcgtta tggtgagttt gaacatttcc tcgacttttc tatcttctca 1380 aaacattcga tttcggtatg ttgattttgc taaccataga tgttttggtg gtgcattaga 1440 catgttgaca gagttggcaa gaagagagaa agaagcaaac atcaaacctg accctgatat 1500 tgatatattc atgaaggtta gttatatcac tcccgaaaca gttaactttg taataatttg 1560 gagtacacaa ctaagtcata acttcattac tccattacag gcgatgtcaa cagcaggtga 1620 aaaaacaaat gtgatgacag attatatcct caaggtatag tttccttcca acttttcttt 1680 tataagagaa ttatcaagtc ttgtacttga catgatctaa actctttgta atttttacag 1740 atcttaggac ttgaggtctg tgcagacact atggtcggcg atgatatgtt gagaggcatc 1800 tccggaggac aaaagaagcg tgtcactact ggtgaaatgc tggttggacc gtctagggct 1860 ctgttcatgg atgagatatc gactggttta gatagttcaa cgacttacca gatagtgaac 1920 tccctcagaa actatgttca tatcttcaat gggacagctc tgatctctct ccttcagcct 1980 gcgccagaga cattcaatct cttcgatgat atcattctca ttgcagaagg cgagatcatc 2040 tacgagggcc ctcgtgatca cgttgtggag ttctttgaga ccatgggatt caaatgtcct 2100 ccaagaaaag gcgttgctga tttccttcaa gaagtaagac ttgattcata agaacacaat 2160 catatgagcg attttgttcc atgtcttaca ctttaacgat cactactctt gcaggtgaca 2220 tcaaagaaag accaaatgca gtactgggca cgacgtgatg agccttacag gttcattaga 2280 gtgagagagt ttgcagaggc gtttcaatca ttccacgttg gccggagaat cggagatgag 2340 cttgctttgc cctttgacaa gacaaagagc catccggctg ctctaaccac caagaaatac 2400 ggagttggga ttaaagaact tgtcaagacc agcttctcaa gagaatactt actcatgaaa 2460 agaaactcct ttgtttacta cttcaagttt ggacaagtaa gtgattacgc atatagtacc 2520 aattcttcac atcttgcaat aatatctcct tatctttaag agagactcat ggaaatgttg 2580 tgcttgcttc cttgtgttta cagctgctgg taatggcatt tttgacaatg acgttgttct 2640 ttcggacgga gatgcaaaag aagactgagg ttgatgggag tctctacact ggagccttgt 2700 tcttcatcct tatgatgctc atgttcaatg gaatgtctga actttcaatg accatagcaa 2760 aacttcctgt gttttacaaa caaagagatc tcctcttcta ccctgcatgg gtgtactctc 2820 tgcctccttg gctcctcaag atacctataa gcttcatgga agccgctctc acaacattca 2880 tcacttacta tgtcatcggc tttgatccca acgttggaag gtacataaat acactaaaca 2940 actaaacatg acaaatttgg atacttatga aggagctgct aagggttctc ctggtttctt 3000 gttggtttgc tgcaggctgt ttaagcagta tattctcctc gtgctcatga accaaatggc 3060 ttcagcattg tttaagatgg tggcagcatt gggaagaaac atgatcgttg caaatacatt 3120 tggtgcattt gcgatgctcg tcttctttgc cttgggtggt gtggtacttt cacgaggtat 3180 gtgttgatgc atatctgttt gcgtccagtt tctcatatca aaagctgatt aattttatcc 3240 attaacctga tcaaaacaga cgacattaag aagtggtgga tatggggtta ctggatctcc 3300 ccaataatgt atggacagaa cgcgatccta gccaatgagt tctttggaca cagctggagt 3360 cgagtaagta tcaccctcct ctgtccactc cagattcgga aatcattttc catggttctg 3420 gtatttcttt tagacaggac tgatagggtt gattgtatta caggctgtcg aaaactcgag 3480 cgaaacactt ggagttactt tccttaagtc tcgtgggttc ttaccccatg catactggta 3540 ctggattgga actggagcct tacttgggtt cgtcgtgtta ttcaattttg gtttcacgct 3600 ggctctgacg tttctgaact gtacgtaatc attccgtttt gcttgttctc atcttctgat 3660 acaaaagctg ttttcttaaa ataaaaaatc cttgtgaact ttgtttgcct acagccttgg 3720 gaaagcctca agctgttatt gcagaagagc ctgcgagtga tgagacagaa cttcagtctg 3780 ctaggtcaga aggtgtagtt gaagctggtg ccaataagaa aagagggatg gtgcttccat 3840 ttgagccaca ttcaattacc ttcgacaatg ttgtatactc agttgacatg ccccaggtaa 3900 taaccttgct cgaggatttc gtatcattaa tgcaagaaac ttcatactga tttttgttgt 3960 ggattctttc tcacaggaaa tgatagagca aggcacacaa gaagacagac ttgtcctgtt 4020 gaaaggtgtg aatggtgcat tcaggccagg cgtgctcacg gctctcatgg gtgtctctgg 4080 agctggcaaa accactctga tggatgttct tgccggaagg aaaaccggtg gttatattga 4140 tggcaacatc accatttccg gttaccctaa gaatcaacaa acatttgccc gtatctcagg 4200 atactgtgaa caaactgata tccattcccc acatgtcact gtttacgagt ccttggttta 4260 ctcagcctgg ctccgattac ctaaagaagt tgataaaaac aagagaaagg ttattcagag 4320 acattatcat ttccagaagt aaaatgagga aatcgaaact atactaacaa tttttgtttt 4380 gtttggtaca gatattcata gaggaagtga tggagctggt ggagttaacg ccgctgaggc 4440 aagcactggt tggactacct ggtgagagcg gtttgtcaac agagcaaaga aagagactga 4500 ccattgcggt ggagctggtt gcaaatcctt ccatcatatt catggatgaa cctacttcag 4560 gattggatgc acgagctgct gccatcgtta tgaggactgt aaggaacaca gttgacactg 4620 gtagaacagt cgtctgcacc attcaccagc ctagcatcga catctttgaa gcctttgatg 4680 aggtattcaa actacattca aaacaaaaaa gtgaaaaaga actttaatca tttgttaaca 4740 tgcttgggta tgcgtatata tgcagttgtt cctacttaag cgtggaggtg aggagatata 4800 cgttggacct cttggccacg aatcaaccca tttgatcaac tattttgagg taataaatac 4860 gactaagaca aacacaccat acttaaaact tgtttggaag acatggactg aatatgttga 4920 ctcggcaaca gagtattcaa ggaatcaaca agatcacaga aggatacaac ccagcaacct 4980 ggatgcttga agtctcaacc acatctcaag aagcggcttt aggagtcgat ttcgcccaag 5040 tctacaaaaa ttcagaactt tacaagtaag tacatcagtc atcatgttga ggttaattca 5100 catattatac agcagaatct gatctcgcca ataacttttc tcttatttag gagaaacaag 5160 gagctaatca aggagctaag ccagccagct ccaggatcaa aagatttata tttcccaaca 5220 caatactctc aatcgttctt gacacaatgt atggcttctc tatggaaaca acactggtcc 5280 tactggagaa atcctcctta cacagccgtg agattcctct tcacaatcgg cattgctctt 5340 atgttcggca caatgttctg ggaccttgga ggcaaaacgt aagctaaaaa aacctcagca 5400 aaattctaga ctttacaata caaaaacaaa actccttatt gaccaagttc gaatattttt 5460 gtcacctcca ggaaaacgag acaggattta tcgaatgcaa tgggttcaat gtacacagct 5520 gttctcttcc tcggattaca aaacgcagct tcagtgcaac cagtcgtcaa cgtcgaaaga 5580 actgtctttt accgagaaca agccgccgga atgtactccg ccatgcctta tgctttcgct 5640 caggtacttc aaaaaaacat catagagagt gaaatttacg atttttcgtt gcggaaacct 5700 caaattcaaa cttcgatttg ttccaggttt tcatcgagat cccatacgtt ctcgtgcaag 5760 cgatagtgta cggtctcata gtgtacgcta tgataggatt cgagtggacg gcggtgaagt 5820 tcttctggta cctcttcttt atgtacggat cattcttaac tttcaccttc tacggaatga 5880 tggctgtagc tatgacgcct aaccaccaca tcgcctccgt cgtctcctcc gctttctacg 5940 gcatctggaa tctcttctcc ggcttcctca tccctcgtcc cgtaagtacc aaccttatta 6000 ttgtttaatt tcaaaatgag ccaaaactta ttgaaatatt aaatattgac cctaaacgtt 6060 tatacgaaaa cagagtatgc ctgtgtggtg ggaatggtac tactggcttt gcccagttgc 6120 atggacattg tatggattaa tcgcatcaca gttcggtgat attacagaac ctatggcaga 6180 tagtaatatg agtgtgaagc aattcattag agaattctat ggatatagag aaggtttctt 6240 gggtgtggtt gccgccatga acgtcatctt tcctttgctc tttgccgtta tctttgctat 6300 cggaatcaag agtttcaatt tccaaaaacg atagacagtt tatagttttg cattctattt 6360 catgtaacac aaataaaaag agactttttg tttatatgct attctttcta tttttgtaat 6420 gccgtattga tattaataaa aggatgatca acaacactgg attagaatg 6469 <210> 2 <211> 6485 <212> DNA <213> Arabidopsis thaliana <400> 2 atggcggcgg cttcgaatgg gagtgagtat ttcgaattcg atgtagaaac aggaagagaa 60 tcgttcgcgc ggccgtctaa cgccgagaca gtggagcaag atgaggaaga tctacggtgg 120 gcggcgatcg gaagattacc gtcgcagaga caagggactc ataatgcgat tctgcgtcgg 180 tcgcaaacgc aaacgcagac ttctggttac gctgatggaa acgtggttca aacgattgat 240 gtgaagaaat tggatcgtgc ggatcgtgag atgttggttc gtcaagctct tgctactagt 300 gatcaagata atttcaagct tctctctgct attaaagaac gtcttgatcg gtttgttacg 360 actcttcgaa ttctctctgt ttctaatttt agggagaaaa agtaaatttt atatatatat 420 atatggctcg tttgaatttg atacaaattt tgttcgtaga gttggtatgg aggttccgaa 480 aatcgaagtg cggttcgaga atttgaatat tgaagctgat gttcaagctg gtacaagagc 540 tttgcctact ttggttaatg tctctcgtga tttctttgag gtttgtcttc tttgttttat 600 cttgtgagta ttctaacaca atcttttttt gatgattgta tctaatctct gtttgttgca 660 gcgttgctta agcagcttga ggataatcaa gcctaggaaa cacaagctta atattttgaa 720 agatattagt gggattatca aaccaggaag gtgaattaaa agactgtttt tagattatcg 780 ctatgtcata tgtatgatca caagtactaa tgttttttgc tacttttgaa ggatgacttt 840 gttgttagga ccaccgggtt ccggaaaatc gacgttgctt cttgctcttg cagggaagct 900 tgataagagt ttaaaggtta gttgatccgt gaagttatta actatgttca tctatatcac 960 atgtaactta tgtttgttat ctcatttcgt tttaaaagtc acgtactcac atgcatgtgt 1020 cctttttgtt ttttgcatct atgacagaaa acgggtaaca ttacctataa tggagagaat 1080 cttaacaagt ttcatgttaa aagaacttca gcatatataa gtcaaacaga taatcacatt 1140 gctgaactca ctgttcgtga aactcttgat ttcgctgcga gatgtcaagg cgcaagcgaa 1200 ggatttgcag gttttttctt atttgatgtt actactcact atgtaaactt cttcatcaag 1260 tcatagattc tcaaatattt tctgttttga tgttgtgtaa ttgtaggtta catgaaagat 1320 ctaacccgat tagagaaaga gaggggtata cgtccttctt ctgagattga tgcgtttatg 1380 aaggttagta tcatatagct caataatctc tcatgtttct tgatcacaca acaaagtttt 1440 gacacatgct ctctgttatt aggctgcttc tgtcaaaggc gaaaagcata gtgtttcgac 1500 agattatgtg cttaaagtac ttggtcttga tgtatgttct gatacaatgg ttggtaatga 1560 tatgatgaga ggtgtttcag gaggtcaaag gaaaagagtt acaacaggtc ttttctcact 1620 ctgtttactt atctatctca cttagaatta tacaccagct ctgttttgct aattgataaa 1680 tttgacacaa tgcaggagaa atgactgttg ggccaagaaa aacgttgttt atggatgaaa 1740 tatctactgg tcttgatagt tcaacaactt tccagattgt caaatgtatc agaaacttcg 1800 ttcatctaat ggatgcaact gttcttatgg cacttcttca gcctgcacca gaaacatttg 1860 acttgtttga tgacttaatt cttctatcag aaggttacat ggtatatcaa ggtcctcgag 1920 aagatgtgat agcctttttt gagtctctag gattccgtct cccaccgcgt aaaggtgtgg 1980 cagattttct ccaagaggta tatatctaaa ctctgttctt taatttgagt tttagtatac 2040 tcaaactcaa tctattataa tgttggtatg caggtgacct ctaaaaagga tcaagctcaa 2100 tactgggcag atccttcgaa gccatatcag ttcattcctg tctcagacat agcggctgca 2160 ttccgtaact cgaaatacgg gcatgctgcg gattccaagc tggctgcacc atttgacaag 2220 aaatctgcgg atccttcagc tttatgcaga acaaaatttg ccatttcagg atgggaaaac 2280 cttaaagttt gctttgtacg agaattatta ttgatcaaac ggcacaaatt tctttacact 2340 tttaggacat gccaggtaaa tatctacatc tttttgctgc atttgtaaat ttggcatcat 2400 ctagagtatt gacacttagg tttgtttcca tgacattggt tttgtgtggc ttttgaatca 2460 agtaaccaat aaattttgtc tataccataa gcctaggtct tcatttgcgc ttgcttcaat 2520 tctgcttttc taaaattagt cttattctta tatatatgca ccattatgtg atacaatttg 2580 catcactata gtgacatctt gacgtagata ttattcaaat gtccttagat aaaattctaa 2640 gattctgatc taaaacattt tgtgttccat aaaggttgga tttgtgggac ttgtcacagc 2700 gacagtgttc ttgaaaacta gattacaccc aacaagcgaa caatttggca atgagtatct 2760 atcttgcctt ttcttcgggc tagtccacat gatgttcaac ggtttctctg aactacctct 2820 catgatatcg cgtctcccgg ttttttacaa gcaaagggat aactcctttc atcctgcttg 2880 gtcatggtct attgccagct ggctcttgcg tgtgccttac tctgtccttg aagctgttgt 2940 ctggagtggt gtagtatact tcactgtggg acttgctccc tcagcaggca ggttggttat 3000 ttatcttgat attattattt ctaaacttct tgttgatttt tagttttgta gtcttacaat 3060 aaatctcttt ttgttctcct agatttttcc gatacatgtt acttctcttc tcggtgcatc 3120 aaatggctct cggtttattt cgtatgatgg cttctctagc aagggacatg gtcattgcca 3180 atacattcgg atctgcagca atcttaatag tgttcttgct tggtggattc gttattccaa 3240 aaggttagtt attactacta cttcactatt tcttgtataa aagaaattta tttgttggtt 3300 agtgttgcta aaataaaacc ttatcatttc ttacagctga tattaaacct tggtgggttt 3360 ggggtttttg ggtgtcgccg ttatcctatg gacaacgtgc cattgcggtc aatgaattca 3420 cagccacacg gtggatgacg gtgtgttcat taatcttttt gttgagttaa tattactttg 3480 ttagaatatg catgcaatgc ttaactatat acttttaaac atcatgcagc catcagctat 3540 atctgatact acaattggac tcaaccttct aaagctacgt agctttccaa cgaatgacta 3600 ttggtattgg attggaatcg cggttctcat tggttatgca attctattca ataacgtagt 3660 aactctcgcc ttggcttatc ttaaccgtga gagtctctat tgctaatggt tctttcgtct 3720 agatatctgt agtataatat tgtgaagctt agctcttttg ttttttctct tgcagctcta 3780 agaaaagctc gagcagttgt tttagacgat cccaatgaag aaactgcttt agtagcagat 3840 gcaaatcaag taataagtga gaaaaaggga atgatccttc cattcaaacc attaacaatg 3900 actttccaca atgtcaacta ttatgttgac atgccaaagg ttaatttcac ttctctgttt 3960 tgctttggta gtatcagcct ttatctatgg ttgcattaaa gtttgtttgt tcgggaaatc 4020 tcaggaaatg cgttctcaag gtgtaccaga gacaagacta caactgttat caaacgtgag 4080 tggagtgttc tcacctggag ttcttacagc tttagttgga tcaagcggtg cgggaaaaac 4140 tacgttgatg gatgttcttg cgggtcgaaa gacaggtgga tataccgagg gagatattag 4200 aatctctggt cacccaaaag aacaacaaac gtttgctagg atctctggat atgttgagca 4260 gaacgatata cactctcctc aagtcacagt tgaagagtcc ttatggtttt cggctagcct 4320 tcgtcttcct aaggagatca ccaaagaaca gaaaaaggtc agtatgaatg agttttacct 4380 catttggttc cgaatatgga ttgtttcact aacatatatt ttcttgtttt gttaggaatt 4440 tgtggaacaa gtcatgagac ttgtagagct tgacactctg agatatgctt tagtaggttt 4500 acctggtacc acgggtcttt ctactgagca gaggaaacgt ctaacaattg cggttgagtt 4560 agtagcaaat ccatcaatta ttttcatgga tgaaccaaca tctggacttg atgcaagagc 4620 agcagcaatt gtgatgagaa ctgttagaaa cactgttgat actggaagaa cagtggtttg 4680 caccattcat caacctagca ttgacatttt tgaggccttt gacgaggttt gacctaattt 4740 tgcttggatt acaagaatag ttatgaattg ttatcagtcg atgatcttaa taacgtctgt 4800 accttttttt ttttgcttaa ccttaaaaat acagctgctt ctaatgaaac gaggaggaca 4860 ggttatatat ggtgggaaat tgggtacaca ctcgcaggtt ctggtagatt actttcaggt 4920 acttacttgt ccctgccttt tcctattctg ttttgatctt cgcacttaca tacttgtgca 4980 ccacgttaaa tatgacaaac cctaaaccct ctacagggga ttaacggagt acctccaatc 5040 tcaagtggtt acaatccagc aacttggatg cttgaagtaa ctacacctgc tttggaagag 5100 aaatataaca tggagtttgc agatttatac aagaaatctg accagtttag gtaactatca 5160 caatccttaa agctacattt tcaaaaacat aaatcaaacc atatactgtg tattttaaac 5220 agagaagtag aggcaaacat caagcaactc agtgttccac cagaaggctc agaaccaata 5280 agctttacat caagatactc acaaaaccaa ctatctcaat ttctactctg tctctggaaa 5340 cagaaccttg tctactggag aagtccagaa tacaatctcg tgagattagt cttcacaacg 5400 atcgctgcgt ttatactggg aacagttttc tgggacattg gttccaagag gacttcctca 5460 caggatttga tcaccgtaat gggagctctc tactcggctt gtctgtttct tggagttagt 5520 aacgcttcat cagtacaacc aatcgtttca atcgaaagaa cagttttcta cagagagaaa 5580 gccgctggta tgtatgctcc aattccatat gcagcagctc agggtcttgt ggagatacct 5640 tacattctga cccaaaccat tctctacggt gtcatcacat acttcaccat tggctttgaa 5700 agaacgttca gtaagtttgt actgtatttg gtgttcatgt tcctcacatt cacctacttc 5760 accttctatg gcatgatggc ggttggtctc accccgaatc agcatttagc cgctgtgatc 5820 tcctctgcgt tttactctct ctggaatctc ctatccggtt tcctcgtcca aaagcctgta 5880 agtatttaat atcactctat gagaggtagc taatatagtt atgcattcga acaatcgaga 5940 actaagtatt gatggattat tgttactaaa tttgattctt gactaatcag ccttaccaca 6000 ttttgaaccc ttataatctt taaccctttg tgtttgtttt ggtaattcag ttgattccag 6060 tgtggtggat atggttctat tacatatgtc cagtggcgtg gacactacaa ggagtaatcc 6120 tttcgcagct tggtgacgtg gaaagcatga tcaacgagcc attgtttcat ggcacggtga 6180 aggagtttat tgaatattac ttcggctaca agccaaacat gattggtgta tctgctgctg 6240 ttcttgtcgg tttttgtgct ctcttcttct cagcatttgc actttcagtc aaatacctca 6300 acttccagag aagataaaaa aagtcctgtg ggactaactt tgatattttt ggcctttttc 6360 actggtgttc cgttttaaga gtagttatga ttctttttaa agttagtttt tgtggacatt 6420 gacgaaaaaa acaaacctaa aatataagat tctgttttgc tctaattagg gttttatagt 6480 tttgt 6485 <210> 3 <211> 3055 <212> DNA <213> Arabidopsis thaliana <400> 3 agacattact tatctccggt aacggacaac cactcgtttc ccgaaacagc aactcacaga 60 ctcacaccac tccagtctcc ggcttaacta ccaccagaga cgattctctc ttccgtcggt 120 tctatgactt cgattctcaa cactgtctcc accatccact cttccagagt tacctccgtc 180 gatcgagtcg gagtcctctc tcttcggaat tcggattccg ttgagttcac tcgccggcgt 240 tctggtttct cgacgttgat ctacgaatca cccggtagtt agcattctgt tggatagatt 300 gatgaatgtt ttcttcgatt ttttttttac tgatcttgtt gtggatctct cgtagggcgg 360 agatttgttg tgcgtgcggc ggagactgat actgataaag gtatgatttt ttagttgttt 420 ttattttctc tctcttcaaa attctctttt caaacactgt ggcgtttgaa tttccgacgg 480 cagttaaatc tcagacacct gacaaggcac cagccggtgg ttcaagcatt aaccagcttc 540 tcggtatcaa aggagcatct caagaaactg taattttgtt catctcctca gaatctttta 600 aattatcata tttgtggata atgatgtgtt agtttaggaa ttttcctact aaaggtaatc 660 tcttttgagg acaagtcttg tttttagctt agaaatgatg tgaaaatgtt gtttgttagc 720 taaaaagagt ttgttgttat attctgtatt cagaataaat ggaagattcg tcttcagctt 780 acaaaaccag tcacttggcc tccactggtt tggggagtcg tctgtggtgc tgctgcttca 840 ggtaatcata cgaacctctt ttggatcatg caatactgta cagaaagttt tttcattttc 900 cttccaattg tttcttctgg cagggaactt tcattggacc ccagaggatg ttgctaagtc 960 gattctttgc atgatgatgt ctggtccttg tcttactggc tatacacagg tctggtttta 1020 cacaacaaaa agctgacttg ttcttattct agtgcatttg cttggtgcta caataaccta 1080 gacttgtcga tttccagaca atcaacgact ggtatgatag agatatcgac gcaattaatg 1140 agccatatcg tccaattcca tctggagcaa tatcagagcc agaggtaact gagacagaac 1200 attgtgagct tttatctctt ttgtgattct gatttctcct tactccttaa aatgcaggtt 1260 attacacaag tctgggtgct attattggga ggtcttggta ttgctggaat attagatgtg 1320 tgggtaagtt ggcccttctg acattaacta gtacagttaa agggcacatc agatttgcta 1380 aaatcttccc ttatcaggca gggcatacca ctcccactgt cttctatctt gctttgggag 1440 gatcattgct atcttatata tactctgctc cacctcttaa ggtaagtttt attcctaact 1500 tccactctct agtgataaga cactccatcc aagttttgga gttttgaata tcgatatctg 1560 aactgatctc attgcagcta aaacaaaatg gatgggttgg aaattttgca cttggagcaa 1620 gctatattag tttgccatgg taagatatct cgtgtatcaa taatatatgg cgttgttctc 1680 atctcattga tttgtttctt gctcacttga ctgataggtg ggctggccaa gcattgtttg 1740 gcactcttac gccagatgtt gttgttctaa cactcttgta cagcatagct ggggtactct 1800 tttggcaaac cttttatgtt gcttttttcg ttatctgttg taatatgctc ttgcttcatg 1860 ttgtaccttt gtgataatgc agttaggaat agccattgtt aacgacttca aaagtgttga 1920 aggagataga gcattaggac ttcagtctct cccagtagct tttggcaccg aaactgcaaa 1980 atggatatgc gttggtgcta tagacattac tcagctttct gttgccggta tgtactatcc 2040 actgtttttg tgcagctgtg gcttctattt cttttccttg atcttatcaa ctggatattc 2100 accaatggta aagcacaaat taatgaagct gaatcaacaa aggcaaaaca taaaagtaca 2160 ttctaatgaa atgagctaat gaagaggagg catctacttt tatgtttcat tagtgtgatt 2220 gatggatttt catttcatgc ttctaaaaca agtattttca acagtgtcat gaaataacag 2280 aacttatatc ttcatttgta cttttactag tggatgagtt acacaatcat tgttatagaa 2340 ccaaatcaaa ggtagagatc atcattagta tatgtctatt ttggttgcag gatatctatt 2400 agcatctggg aaaccttatt atgcgttggc gttggttgct ttgatcattc ctcagattgt 2460 gttccaggta aagacgttaa cagtctcaca ttataattaa tcaaattctt gtcactcgtc 2520 tgattgctac actcgcttct ataaactgca gtttaaatac tttctcaagg accctgtcaa 2580 atacgacgtc aagtaccagg taagtcaact tagtacacat gtttgtgttc ttttgaaata 2640 tctttgagag gtctcttaat cagaagttgc ttgaaacact catcttgatt acaggcaagc 2700 gcgcagccat tcttggtgct cggaatattt gtaacggcat tagcatcgca acactgaaaa 2760 aggcgtattt tgatggggtt ttgtcgaaag cagaggtgtt gacacatcaa atgtgggcaa 2820 gtgatggcat caactagttt aaaagatttt gtaaaatgta tgtaccgtta ttactagaaa 2880 caactcctgt tgtatcaatt tagcaaaacg gctgagaaat tgtaattgat gttaccgtat 2940 ttgcgctcca tttttgcatt tcctgctcat atcgaggatt ggggtttatg ttagttctgt 3000 cacttctctg ctttcagaat gtttttgttt tctgtagtgg attttaacta ttttc 3055 <210> 4 <211> 1917 <212> DNA <213> Arabidopsis thaliana <400> 4 gttacacgcg taggctagtg gaaaagccca ccacacataa cacacacaca tcttcttcct 60 ctctttatct tcttcttctc tgtttctcta agaagataga acaatcccaa gattctcctc 120 ttatttgatc aaccatgtcg aatgtcttgc tatccccaaa cggctacgta tttgcatcgc 180 ctaaacctct tggcagattc atcaattcaa agtccggcgg acggaaactt ttcttctccg 240 tcgttagagc ttcttccgat gatgctgatt gtaatgccga agaatgtgct cccgagaagg 300 aggtactcaa tcccacctct gtatttgttt cattctccgc tcaattttgt ttctttaaca 360 aaacccatga tgataatttt tttgcaattg ggttgttcaa cattgctggg aaatatgagt 420 tggttactta gaagatttag tttcggaatc tccaaaatta gctaattagt gaaactggat 480 tataaagtgg ggatttttac aattgaaatg atgtcattga tgaggtttgg tgggtgaaac 540 gcaacaggta ggaacagtga gtatggaatg gttagctgga gagaagacga aagtggtggg 600 aacatttcct cctcggaagc cgcgtggttg gactggctat gttgagaaag atactgctgg 660 tcagactaat gtttactcca ttgaggttcg ttcctcttta tttctccatc tatctatcca 720 ctccactcat ctctactttt attagtttag gttataccta agtaagtttt agtatcttta 780 gggaagcaca ggcactcctt ttaggctcat agtgtttgga gggatactgg tttttagttg 840 agagaaaaag cttcaaactt tgccgaggga acaggttctt atgattctgt gataggcatt 900 agaatgtagt ggatgcataa caacaagatg agattagggg tatttgacca gtggcttgtt 960 tgtgagcctt ttggctttca gggagttagg agatctcttt aaccaatttt caatatacat 1020 ttggctcttt ctttgagttt gatcgttgct tcaaggataa caaagaaaac taggctttcg 1080 atctgatcag ttttactgtt atattttgtc tcgtttgaaa ttaacttttt tttggctttt 1140 cttgtgtgtg tgtgtgtttg ttccataatt ctgtcatcga tctcgacctc catctccaag 1200 aaatatgcat tagcttgcaa caacatgtca tgtaaacccc gcatcttctt gagatggggt 1260 tcttgatcta attcgattgg ctttttctta tcgactcaat tccattggct ttttttctaa 1320 agaatagcgt ttcttgtaac tttttcagcc tgcagtatat gttgcagaga gcgcgattag 1380 ctcaggtact gcaggatcat cggctgatgg ggctgagaac acagcagcaa tcgtagcagg 1440 cattgctctt atcgcagtcg ctgcagcttc ctctatactc ctccaagtcg ggaaagatgc 1500 cccaactcga cccaaagcag tggattacag tggaccgtct cttagctact acatcaacaa 1560 gttcaagcct tctgaaattg ttcaaccttc taccccctcc gtcacagaag ccccaccggt 1620 ggccgagcta gaaacttcac tgccagaaac tccatcggtg gctcagcaag aaacctcact 1680 gccggaaact atggctagtg aggctcagcc agaggcgtct tctgttccaa caacaagtag 1740 tacctcttag gggcttagcc taggattata tctttgtgta aaaatgagat gaagcatata 1800 ccaagattat gcattctgtt aatattcgga tatacctata tgtatgtata cactctgtca 1860 ctcttctctt tgagtgaatg attatatcaa atctcaaatc ctattatttt tgctttt 1917 <210> 5 <211> 2089 <212> DNA <213> Arabidopsis thaliana <400> 5 ctccgcctta tcttgttgac tatctctgta ctacttacga gcgcaagtga gagagtaaca 60 gagaaacgta gagatatagc aaaatggctg cgacgcttac tggatcaggg attgctctag 120 ggttttcgtg ctccgcaaag ttctctaaga gagcttcttc gtcgtccaac cgtcgctgca 180 tcaagatgtc ggtttcggta gaggagaaga cgaagaaatt cactcttcag aaatctgagg 240 aagctttcaa tgctgcgaag gttaggcttt tcaatttcca tgaattcagt caataagaaa 300 tgtttcttca gattgattac gccttgatgt tctgttgctt tggttttgtg ggatttgatc 360 atatttttgg ttaaagtgat tatctttgtt acccttcaag gaacattgct aacaaaataa 420 gattacttga gatagaatca taatctgaga taaagttttt gtttttattg gtttcattct 480 gttctgatta ttgaaattgg tttggttcct ttgtgtagaa cttaatgcct ggaggtgtga 540 actcacctgt acgtgctttc aaatctgttg gtggacaacc agtggtgatg gattctgcaa 600 agggctcacg aataagagac attgatggaa atgaatacat tgactatgtt ggatcttggg 660 gacctgctat aattggtcat gctgatgatg aggtttgttt ctttccagtt ttgatgattt 720 ttagatagct gaaagtttag tagttgatac ggtgatgtgt ctagtgcagg ttcttgctgc 780 tttggctgag acaatgaaga aaggaacaag ctttggtgct ccttgtctct tagagaatgt 840 tcttgctgag atggtgattt cagctgttcc aagtattgaa atggttcggt ttgttaactc 900 cggtacagag gcatgtatgg gtgtgctacg tcttgctcgt gccttcacag ggaaacaaaa 960 gttcatcaag tttgaaggtt gttatcatgg tcatgcaaac tctttccttg tcaaagcagg 1020 tagtggtgta gctactttgg gtctacctga ctcgcctggg gtccccaaag cagctacttc 1080 agatacttta acagctccat acaatgatat tgctgctgtt gagaagcttt ttgaggcaaa 1140 caaaggagag attgctgcca tcattcttga acctgttgtt ggtaactcgg gttttattac 1200 acctaaacca gagttcattg agggaatacg ccggatcact aaagacaatg gtgctcttct 1260 tattttcgat gaagtcatga ctggttttcg tttagcctat ggtggagctc aagaatactt 1320 tggaatcaca cctgacttaa caactcttgg gaaaatcatc ggtggtggtc ttcctgtggg 1380 agcatacggt ggaagaagag acatcatgga aatggtaaag ctagagttgt taacaattga 1440 accgaataat cttttgaatt tgctgcaaga gattgagaaa gtcctgatcc tttatcactc 1500 atgtcttttt gcaggttgca cccgcaggac cgatgtatca agctggtacg ctaagtggta 1560 atccgttggc tatgacagcg ggtatacaca cgctgaagcg gttaagtcag ccagggacat 1620 atgaatactt agacaagatc acaaaagagc ttacaaatgg gatactagaa gccgggaaga 1680 aaaccgggca tgcgatgtgt ggcggttaca taagcgggat gttcggtttc ttcttcacgg 1740 aaggacccgt ctatgatttc tcggatgcaa agaagagtga tacagagaag tttggaaagt 1800 ttttcagagg aatgttggaa gaaggtgtct acttggcacc ttctcaattc gaggccggtt 1860 ttactagctt ggctcacact tcagaagaca tccaattcac tatcgcagcg gccgagaagg 1920 ttctaagtcg gctctaaaat gtctctggaa gcttccaggg aaccatgaat gtatttgaaa 1980 ttttttgatg tagagaaaat tagattcaat tgagagcaag caagcaatct cacaaaattt 2040 aactgtggta agaaaagtca aaatgtctat gaagatttgt tgacttttt 2089 <210> 6 <211> 1082 <212> DNA <213> Arabidopsis thaliana <400> 6 cacttgattc tctcttcctt catcaatcaa tctcgtctcc tggaaaacat tagggagcct 60 ctcagatcct caagaaaacc ctaaatcaaa agtcgagatc gatggcgtcc ttcgctcgcc 120 gtgccatgag cttagctcag atcccatctg ctcgtatttc ctctggagcc gcttctattg 180 gtcagcgtcg ttgcctcgcc ggcgccgctg gtaaattctc tctccctctt ccttctcttt 240 tcgatattta cgattttttt cggtatcaat tttggtggcg tatagcttat ttcgcacatt 300 ttatgtctaa tttgtgtttt cacgaatttt agatgaacca aatgttacaa ctttgtgaag 360 attttgtata tcattggcgt tgtcaggttc caaattgaat cactgtatac aaaaaattga 420 gtttttgagc attttgtgga tttgtctgtg tactgtgcag atcaccatgg atctaccaag 480 gttgacttct ggaaacagcc cacgaaccca ggaaactgga aagaggagca tgtatgtatc 540 aatcatgtgt ttgtgacact gtgtttttgt taatgaaagc tgtgattttt tgttgaattg 600 attccttctg ttacctgacc aatctgcagt ttgtgcttat ttctctgtct ggctggggct 660 tacttttcta cagtggatac aagctcgcca ctgggggaaa aaaagaggta cttactcttg 720 ctcttcagct cttgctcttc ttccattgcc tagaggcttc gggttccttc ccttatacat 780 agtttttttt gcagacgcct gtggagagta cacaatgagt ttcattgtca ccaggaagac 840 cttttatgga gagacctctc attctcatgc actttgtttt cccttttagc agattgtgac 900 tggtcttgtg tttcattttg aaaataattt gcatcacttt tgagatctga tgatgtctgc 960 aatggataaa ttcttgacag ttttgtgttt cataatatcc tatgtttgaa acagacagga 1020 tttaagcaaa tcattgtctc actacgtatg ttgatatatg ctttcgagct atttccttcc 1080 at 1082 <110> DAESANG CORPORATION <120> Process for improving chlorella and chlorella obtained by using the same <130> PN0718 <160> 6 <170> KopatentIn 2.0 <210> 1 <211> 6469 <212> DNA <213> Arabidopsis thaliana <400> 1 atatcatctt tcatctacaa tttctctctt gagtttcttt tcatgtgtta aagtcttatt 60 gtcttgctaa attctttaaa tctttcatct gacacaaaca aaaaaagaga gaagaaaaaa 120 aagaagaaga ctttgatttc ttggatacaa aatggaggga actagttttc accaagcgag 180 taatagtatg agaagaaact catcggtgtg gaagaaagat tcaggaaggg agattttctc 240 gaggtcatct agagaagaag acgatgaaga agctttgaga tgggctgctc ttgagaagct 300 tcccactttt gatcgtctca ggaaaggaat cctaactgcc tcacatgccg gaggacccat 360 caacgagatc gatattcaga agcttgggtt tcaagatact aagaaactgc tagagaggct 420 catcaaagtc ggtgacgatg agcatgagaa actcctctgg aaactcaaga aacgtatcga 480 taggtataca aacttctctc tttttttttt ttagtttagc ttttaatggg aacagaggag 540 ctctgttttt gtttgatgtt catgttggag acttaagtgt taattgtatg taattatgaa 600 aatgacagag ttggaatcga tcttccgaca atagaagttc ggtttgatca tctaaaagtt 660 gaagcagagg ttcatgttgg aggcagagct ttacctacgt tcgtcaattt catctccaat 720 tttgctgatg taagaatgcg ttttttttac ttatctgttt cttgtgtcca aacaaaaact 780 agatctctaa cagtggaatt ttcgttaata ttaacagaag ttcctgaata ctctgcatct 840 tgttccgaac cgaaagaaga agttcactat actcaacgac gtcagcggaa tcgtcaagcc 900 tggcaggtga attttcagat tctgtttcac cattttaaaa tattggaaag tatttttagt 960 tatacttgca cttgttgacc catctttgaa ttctacagga tggctctgct tttgggtcct 1020 ccaagttctg ggaaaacgac cctcttgctt gccttggcgg gaaagcttga tcaagaacta 1080 aaggtaacca tctttccctc tctctatctc tgtttcaact tcatggatat gtttatatga 1140 tctgtgtgaa tcaattgtaa actttgttct ttgattttgt agcaaactgg aagagtgaca 1200 tacaatggtc atggaatgaa cgagtttgtg ccacaaagaa cagctgcata tatcggccaa 1260 aacgatgttc atatcggtga gatgactgtt cgtgagactt ttgcttacgc agctcgcttc 1320 caaggtgttg gttcgcgtta tggtgagttt gaacatttcc tcgacttttc tatcttctca 1380 aaacattcga tttcggtatg ttgattttgc taaccataga tgttttggtg gtgcattaga 1440 catgttgaca gagttggcaa gaagagagaa agaagcaaac atcaaacctg accctgatat 1500 tgatatattc atgaaggtta gttatatcac tcccgaaaca gttaactttg taataatttg 1560 gagtacacaa ctaagtcata acttcattac tccattacag gcgatgtcaa cagcaggtga 1620 aaaaacaaat gtgatgacag attatatcct caaggtatag tttccttcca acttttcttt 1680 tataagagaa ttatcaagtc ttgtacttga catgatctaa actctttgta atttttacag 1740 atcttaggac ttgaggtctg tgcagacact atggtcggcg atgatatgtt gagaggcatc 1800 tccggaggac aaaagaagcg tgtcactact ggtgaaatgc tggttggacc gtctagggct 1860 ctgttcatgg atgagatatc gactggttta gatagttcaa cgacttacca gatagtgaac 1920 tccctcagaa actatgttca tatcttcaat gggacagctc tgatctctct ccttcagcct 1980 gcgccagaga cattcaatct cttcgatgat atcattctca ttgcagaagg cgagatcatc 2040 tacgagggcc ctcgtgatca cgttgtggag ttctttgaga ccatgggatt caaatgtcct 2100 ccaagaaaag gcgttgctga tttccttcaa gaagtaagac ttgattcata agaacacaat 2160 catatgagcg attttgttcc atgtcttaca ctttaacgat cactactctt gcaggtgaca 2220 tcaaagaaag accaaatgca gtactgggca cgacgtgatg agccttacag gttcattaga 2280 gtgagagagt ttgcagaggc gtttcaatca ttccacgttg gccggagaat cggagatgag 2340 cttgctttgc cctttgacaa gacaaagagc catccggctg ctctaaccac caagaaatac 2400 ggagttggga ttaaagaact tgtcaagacc agcttctcaa gagaatactt actcatgaaa 2460 agaaactcct ttgtttacta cttcaagttt ggacaagtaa gtgattacgc atatagtacc 2520 aattcttcac atcttgcaat aatatctcct tatctttaag agagactcat ggaaatgttg 2580 tgcttgcttc cttgtgttta cagctgctgg taatggcatt tttgacaatg acgttgttct 2640 ttcggacgga gatgcaaaag aagactgagg ttgatgggag tctctacact ggagccttgt 2700 tcttcatcct tatgatgctc atgttcaatg gaatgtctga actttcaatg accatagcaa 2760 aacttcctgt gttttacaaa caaagagatc tcctcttcta ccctgcatgg gtgtactctc 2820 tgcctccttg gctcctcaag atacctataa gcttcatgga agccgctctc acaacattca 2880 tcacttacta tgtcatcggc tttgatccca acgttggaag gtacataaat acactaaaca 2940 actaaacatg acaaatttgg atacttatga aggagctgct aagggttctc ctggtttctt 3000 gttggtttgc tgcaggctgt ttaagcagta tattctcctc gtgctcatga accaaatggc 3060 ttcagcattg tttaagatgg tggcagcatt gggaagaaac atgatcgttg caaatacatt 3120 tggtgcattt gcgatgctcg tcttctttgc cttgggtggt gtggtacttt cacgaggtat 3180 gtgttgatgc atatctgttt gcgtccagtt tctcatatca aaagctgatt aattttatcc 3240 attaacctga tcaaaacaga cgacattaag aagtggtgga tatggggtta ctggatctcc 3300 ccaataatgt atggacagaa cgcgatccta gccaatgagt tctttggaca cagctggagt 3360 cgagtaagta tcaccctcct ctgtccactc cagattcgga aatcattttc catggttctg 3420 gtatttcttt tagacaggac tgatagggtt gattgtatta caggctgtcg aaaactcgag 3480 cgaaacactt ggagttactt tccttaagtc tcgtgggttc ttaccccatg catactggta 3540 ctggattgga actggagcct tacttgggtt cgtcgtgtta ttcaattttg gtttcacgct 3600 ggctctgacg tttctgaact gtacgtaatc attccgtttt gcttgttctc atcttctgat 3660 acaaaagctg ttttcttaaa ataaaaaatc cttgtgaact ttgtttgcct acagccttgg 3720 gaaagcctca agctgttatt gcagaagagc ctgcgagtga tgagacagaa cttcagtctg 3780 ctaggtcaga aggtgtagtt gaagctggtg ccaataagaa aagagggatg gtgcttccat 3840 ttgagccaca ttcaattacc ttcgacaatg ttgtatactc agttgacatg ccccaggtaa 3900 taaccttgct cgaggatttc gtatcattaa tgcaagaaac ttcatactga tttttgttgt 3960 ggattctttc tcacaggaaa tgatagagca aggcacacaa gaagacagac ttgtcctgtt 4020 gaaaggtgtg aatggtgcat tcaggccagg cgtgctcacg gctctcatgg gtgtctctgg 4080 agctggcaaa accactctga tggatgttct tgccggaagg aaaaccggtg gttatattga 4140 tggcaacatc accatttccg gttaccctaa gaatcaacaa acatttgccc gtatctcagg 4200 atactgtgaa caaactgata tccattcccc acatgtcact gtttacgagt ccttggttta 4260 ctcagcctgg ctccgattac ctaaagaagt tgataaaaac aagagaaagg ttattcagag 4320 acattatcat ttccagaagt aaaatgagga aatcgaaact atactaacaa tttttgtttt 4380 gtttggtaca gatattcata gaggaagtga tggagctggt ggagttaacg ccgctgaggc 4440 aagcactggt tggactacct ggtgagagcg gtttgtcaac agagcaaaga aagagactga 4500 ccattgcggt ggagctggtt gcaaatcctt ccatcatatt catggatgaa cctacttcag 4560 gattggatgc acgagctgct gccatcgtta tgaggactgt aaggaacaca gttgacactg 4620 gtagaacagt cgtctgcacc attcaccagc ctagcatcga catctttgaa gcctttgatg 4680 aggtattcaa actacattca aaacaaaaaa gtgaaaaaga actttaatca tttgttaaca 4740 tgcttgggta tgcgtatata tgcagttgtt cctacttaag cgtggaggtg aggagatata 4800 cgttggacct cttggccacg aatcaaccca tttgatcaac tattttgagg taataaatac 4860 gactaagaca aacacaccat acttaaaact tgtttggaag acatggactg aatatgttga 4920 ctcggcaaca gagtattcaa ggaatcaaca agatcacaga aggatacaac ccagcaacct 4980 ggatgcttga agtctcaacc acatctcaag aagcggcttt aggagtcgat ttcgcccaag 5040 tctacaaaaa ttcagaactt tacaagtaag tacatcagtc atcatgttga ggttaattca 5100 catattatac agcagaatct gatctcgcca ataacttttc tcttatttag gagaaacaag 5160 gagctaatca aggagctaag ccagccagct ccaggatcaa aagatttata tttcccaaca 5220 caatactctc aatcgttctt gacacaatgt atggcttctc tatggaaaca acactggtcc 5280 tactggagaa atcctcctta cacagccgtg agattcctct tcacaatcgg cattgctctt 5340 atgttcggca caatgttctg ggaccttgga ggcaaaacgt aagctaaaaa aacctcagca 5400 aaattctaga ctttacaata caaaaacaaa actccttatt gaccaagttc gaatattttt 5460 gtcacctcca ggaaaacgag acaggattta tcgaatgcaa tgggttcaat gtacacagct 5520 gttctcttcc tcggattaca aaacgcagct tcagtgcaac cagtcgtcaa cgtcgaaaga 5580 actgtctttt accgagaaca agccgccgga atgtactccg ccatgcctta tgctttcgct 5640 caggtacttc aaaaaaacat catagagagt gaaatttacg atttttcgtt gcggaaacct 5700 caaattcaaa cttcgatttg ttccaggttt tcatcgagat cccatacgtt ctcgtgcaag 5760 cgatagtgta cggtctcata gtgtacgcta tgataggatt cgagtggacg gcggtgaagt 5820 tcttctggta cctcttcttt atgtacggat cattcttaac tttcaccttc tacggaatga 5880 tggctgtagc tatgacgcct aaccaccaca tcgcctccgt cgtctcctcc gctttctacg 5940 gcatctggaa tctcttctcc ggcttcctca tccctcgtcc cgtaagtacc aaccttatta 6000 ttgtttaatt tcaaaatgag ccaaaactta ttgaaatatt aaatattgac cctaaacgtt 6060 tatacgaaaa cagagtatgc ctgtgtggtg ggaatggtac tactggcttt gcccagttgc 6120 atggacattg tatggattaa tcgcatcaca gttcggtgat attacagaac ctatggcaga 6180 tagtaatatg agtgtgaagc aattcattag agaattctat ggatatagag aaggtttctt 6240 gggtgtggtt gccgccatga acgtcatctt tcctttgctc tttgccgtta tctttgctat 6300 cggaatcaag agtttcaatt tccaaaaacg atagacagtt tatagttttg cattctattt 6360 catgtaacac aaataaaaag agactttttg tttatatgct attctttcta tttttgtaat 6420 gccgtattga tattaataaa aggatgatca acaacactgg attagaatg 6469 <210> 2 <211> 6485 <212> DNA <213> Arabidopsis thaliana <400> 2 atggcggcgg cttcgaatgg gagtgagtat ttcgaattcg atgtagaaac aggaagagaa 60 tcgttcgcgc ggccgtctaa cgccgagaca gtggagcaag atgaggaaga tctacggtgg 120 gcggcgatcg gaagattacc gtcgcagaga caagggactc ataatgcgat tctgcgtcgg 180 tcgcaaacgc aaacgcagac ttctggttac gctgatggaa acgtggttca aacgattgat 240 gtgaagaaat tggatcgtgc ggatcgtgag atgttggttc gtcaagctct tgctactagt 300 gatcaagata atttcaagct tctctctgct attaaagaac gtcttgatcg gtttgttacg 360 actcttcgaa ttctctctgt ttctaatttt agggagaaaa agtaaatttt atatatatat 420 atatggctcg tttgaatttg atacaaattt tgttcgtaga gttggtatgg aggttccgaa 480 aatcgaagtg cggttcgaga atttgaatat tgaagctgat gttcaagctg gtacaagagc 540 tttgcctact ttggttaatg tctctcgtga tttctttgag gtttgtcttc tttgttttat 600 cttgtgagta ttctaacaca atcttttttt gatgattgta tctaatctct gtttgttgca 660 gcgttgctta agcagcttga ggataatcaa gcctaggaaa cacaagctta atattttgaa 720 agatattagt gggattatca aaccaggaag gtgaattaaa agactgtttt tagattatcg 780 ctatgtcata tgtatgatca caagtactaa tgttttttgc tacttttgaa ggatgacttt 840 gttgttagga ccaccgggtt ccggaaaatc gacgttgctt cttgctcttg cagggaagct 900 tgataagagt ttaaaggtta gttgatccgt gaagttatta actatgttca tctatatcac 960 atgtaactta tgtttgttat ctcatttcgt tttaaaagtc acgtactcac atgcatgtgt 1020 cctttttgtt ttttgcatct atgacagaaa acgggtaaca ttacctataa tggagagaat 1080 cttaacaagt ttcatgttaa aagaacttca gcatatataa gtcaaacaga taatcacatt 1140 gctgaactca ctgttcgtga aactcttgat ttcgctgcga gatgtcaagg cgcaagcgaa 1200 ggatttgcag gttttttctt atttgatgtt actactcact atgtaaactt cttcatcaag 1260 tcatagattc tcaaatattt tctgttttga tgttgtgtaa ttgtaggtta catgaaagat 1320 ctaacccgat tagagaaaga gaggggtata cgtccttctt ctgagattga tgcgtttatg 1380 aaggttagta tcatatagct caataatctc tcatgtttct tgatcacaca acaaagtttt 1440 gacacatgct ctctgttatt aggctgcttc tgtcaaaggc gaaaagcata gtgtttcgac 1500 agattatgtg cttaaagtac ttggtcttga tgtatgttct gatacaatgg ttggtaatga 1560 tatgatgaga ggtgtttcag gaggtcaaag gaaaagagtt acaacaggtc ttttctcact 1620 ctgtttactt atctatctca cttagaatta tacaccagct ctgttttgct aattgataaa 1680 tttgacacaa tgcaggagaa atgactgttg ggccaagaaa aacgttgttt atggatgaaa 1740 tatctactgg tcttgatagt tcaacaactt tccagattgt caaatgtatc agaaacttcg 1800 ttcatctaat ggatgcaact gttcttatgg cacttcttca gcctgcacca gaaacatttg 1860 acttgtttga tgacttaatt cttctatcag aaggttacat ggtatatcaa ggtcctcgag 1920 aagatgtgat agcctttttt gagtctctag gattccgtct cccaccgcgt aaaggtgtgg 1980 cagattttct ccaagaggta tatatctaaa ctctgttctt taatttgagt tttagtatac 2040 tcaaactcaa tctattataa tgttggtatg caggtgacct ctaaaaagga tcaagctcaa 2100 tactgggcag atccttcgaa gccatatcag ttcattcctg tctcagacat agcggctgca 2160 ttccgtaact cgaaatacgg gcatgctgcg gattccaagc tggctgcacc atttgacaag 2220 aaatctgcgg atccttcagc tttatgcaga acaaaatttg ccatttcagg atgggaaaac 2280 cttaaagttt gctttgtacg agaattatta ttgatcaaac ggcacaaatt tctttacact 2340 tttaggacat gccaggtaaa tatctacatc tttttgctgc atttgtaaat ttggcatcat 2400 ctagagtatt gacacttagg tttgtttcca tgacattggt tttgtgtggc ttttgaatca 2460 agtaaccaat aaattttgtc tataccataa gcctaggtct tcatttgcgc ttgcttcaat 2520 tctgcttttc taaaattagt cttattctta tatatatgca ccattatgtg atacaatttg 2580 catcactata gtgacatctt gacgtagata ttattcaaat gtccttagat aaaattctaa 2640 gattctgatc taaaacattt tgtgttccat aaaggttgga tttgtgggac ttgtcacagc 2700 gacagtgttc ttgaaaacta gattacaccc aacaagcgaa caatttggca atgagtatct 2760 atcttgcctt ttcttcgggc tagtccacat gatgttcaac ggtttctctg aactacctct 2820 catgatatcg cgtctcccgg ttttttacaa gcaaagggat aactcctttc atcctgcttg 2880 gtcatggtct attgccagct ggctcttgcg tgtgccttac tctgtccttg aagctgttgt 2940 ctggagtggt gtagtatact tcactgtggg acttgctccc tcagcaggca ggttggttat 3000 ttatcttgat attattattt ctaaacttct tgttgatttt tagttttgta gtcttacaat 3060 aaatctcttt ttgttctcct agatttttcc gatacatgtt acttctcttc tcggtgcatc 3120 aaatggctct cggtttattt cgtatgatgg cttctctagc aagggacatg gtcattgcca 3180 atacattcgg atctgcagca atcttaatag tgttcttgct tggtggattc gttattccaa 3240 aaggttagtt attactacta cttcactatt tcttgtataa aagaaattta tttgttggtt 3300 agtgttgcta aaataaaacc ttatcatttc ttacagctga tattaaacct tggtgggttt 3360 ggggtttttg ggtgtcgccg ttatcctatg gacaacgtgc cattgcggtc aatgaattca 3420 cagccacacg gtggatgacg gtgtgttcat taatcttttt gttgagttaa tattactttg 3480 ttagaatatg catgcaatgc ttaactatat acttttaaac atcatgcagc catcagctat 3540 atctgatact acaattggac tcaaccttct aaagctacgt agctttccaa cgaatgacta 3600 ttggtattgg attggaatcg cggttctcat tggttatgca attctattca ataacgtagt 3660 aactctcgcc ttggcttatc ttaaccgtga gagtctctat tgctaatggt tctttcgtct 3720 agatatctgt agtataatat tgtgaagctt agctcttttg ttttttctct tgcagctcta 3780 agaaaagctc gagcagttgt tttagacgat cccaatgaag aaactgcttt agtagcagat 3840 gcaaatcaag taataagtga gaaaaaggga atgatccttc cattcaaacc attaacaatg 3900 actttccaca atgtcaacta ttatgttgac atgccaaagg ttaatttcac ttctctgttt 3960 tgctttggta gtatcagcct ttatctatgg ttgcattaaa gtttgtttgt tcgggaaatc 4020 tcaggaaatg cgttctcaag gtgtaccaga gacaagacta caactgttat caaacgtgag 4080 tggagtgttc tcacctggag ttcttacagc tttagttgga tcaagcggtg cgggaaaaac 4140 tacgttgatg gatgttcttg cgggtcgaaa gacaggtgga tataccgagg gagatattag 4200 aatctctggt cacccaaaag aacaacaaac gtttgctagg atctctggat atgttgagca 4260 gaacgatata cactctcctc aagtcacagt tgaagagtcc ttatggtttt cggctagcct 4320 tcgtcttcct aaggagatca ccaaagaaca gaaaaaggtc agtatgaatg agttttacct 4380 catttggttc cgaatatgga ttgtttcact aacatatatt ttcttgtttt gttaggaatt 4440 tgtggaacaa gtcatgagac ttgtagagct tgacactctg agatatgctt tagtaggttt 4500 acctggtacc acgggtcttt ctactgagca gaggaaacgt ctaacaattg cggttgagtt 4560 agtagcaaat ccatcaatta ttttcatgga tgaaccaaca tctggacttg atgcaagagc 4620 agcagcaatt gtgatgagaa ctgttagaaa cactgttgat actggaagaa cagtggtttg 4680 caccattcat caacctagca ttgacatttt tgaggccttt gacgaggttt gacctaattt 4740 tgcttggatt acaagaatag ttatgaattg ttatcagtcg atgatcttaa taacgtctgt 4800 accttttttt ttttgcttaa ccttaaaaat acagctgctt ctaatgaaac gaggaggaca 4860 ggttatatat ggtgggaaat tgggtacaca ctcgcaggtt ctggtagatt actttcaggt 4920 acttacttgt ccctgccttt tcctattctg ttttgatctt cgcacttaca tacttgtgca 4980 ccacgttaaa tatgacaaac cctaaaccct ctacagggga ttaacggagt acctccaatc 5040 tcaagtggtt acaatccagc aacttggatg cttgaagtaa ctacacctgc tttggaagag 5100 aaatataaca tggagtttgc agatttatac aagaaatctg accagtttag gtaactatca 5160 caatccttaa agctacattt tcaaaaacat aaatcaaacc atatactgtg tattttaaac 5220 agagaagtag aggcaaacat caagcaactc agtgttccac cagaaggctc agaaccaata 5280 agctttacat caagatactc acaaaaccaa ctatctcaat ttctactctg tctctggaaa 5340 cagaaccttg tctactggag aagtccagaa tacaatctcg tgagattagt cttcacaacg 5400 atcgctgcgt ttatactggg aacagttttc tgggacattg gttccaagag gacttcctca 5460 caggatttga tcaccgtaat gggagctctc tactcggctt gtctgtttct tggagttagt 5520 aacgcttcat cagtacaacc aatcgtttca atcgaaagaa cagttttcta cagagagaaa 5580 gccgctggta tgtatgctcc aattccatat gcagcagctc agggtcttgt ggagatacct 5640 tacattctga cccaaaccat tctctacggt gtcatcacat acttcaccat tggctttgaa 5700 agaacgttca gtaagtttgt actgtatttg gtgttcatgt tcctcacatt cacctacttc 5760 accttctatg gcatgatggc ggttggtctc accccgaatc agcatttagc cgctgtgatc 5820 tcctctgcgt tttactctct ctggaatctc ctatccggtt tcctcgtcca aaagcctgta 5880 agtatttaat atcactctat gagaggtagc taatatagtt atgcattcga acaatcgaga 5940 actaagtatt gatggattat tgttactaaa tttgattctt gactaatcag ccttaccaca 6000 ttttgaaccc ttataatctt taaccctttg tgtttgtttt ggtaattcag ttgattccag 6060 tgtggtggat atggttctat tacatatgtc cagtggcgtg gacactacaa ggagtaatcc 6120 tttcgcagct tggtgacgtg gaaagcatga tcaacgagcc attgtttcat ggcacggtga 6180 aggagtttat tgaatattac ttcggctaca agccaaacat gattggtgta tctgctgctg 6240 ttcttgtcgg tttttgtgct ctcttcttct cagcatttgc actttcagtc aaatacctca 6300 acttccagag aagataaaaa aagtcctgtg ggactaactt tgatattttt ggcctttttc 6360 actggtgttc cgttttaaga gtagttatga ttctttttaa agttagtttt tgtggacatt 6420 gacgaaaaaa acaaacctaa aatataagat tctgttttgc tctaattagg gttttatagt 6480 tttgt 6485 <210> 3 <211> 3055 <212> DNA <213> Arabidopsis thaliana <400> 3 agacattact tatctccggt aacggacaac cactcgtttc ccgaaacagc aactcacaga 60 ctcacaccac tccagtctcc ggcttaacta ccaccagaga cgattctctc ttccgtcggt 120 tctatgactt cgattctcaa cactgtctcc accatccact cttccagagt tacctccgtc 180 gatcgagtcg gagtcctctc tcttcggaat tcggattccg ttgagttcac tcgccggcgt 240 tctggtttct cgacgttgat ctacgaatca cccggtagtt agcattctgt tggatagatt 300 gatgaatgtt ttcttcgatt ttttttttac tgatcttgtt gtggatctct cgtagggcgg 360 agatttgttg tgcgtgcggc ggagactgat actgataaag gtatgatttt ttagttgttt 420 ttattttctc tctcttcaaa attctctttt caaacactgt ggcgtttgaa tttccgacgg 480 cagttaaatc tcagacacct gacaaggcac cagccggtgg ttcaagcatt aaccagcttc 540 tcggtatcaa aggagcatct caagaaactg taattttgtt catctcctca gaatctttta 600 aattatcata tttgtggata atgatgtgtt agtttaggaa ttttcctact aaaggtaatc 660 tcttttgagg acaagtcttg tttttagctt agaaatgatg tgaaaatgtt gtttgttagc 720 taaaaagagt ttgttgttat attctgtatt cagaataaat ggaagattcg tcttcagctt 780 acaaaaccag tcacttggcc tccactggtt tggggagtcg tctgtggtgc tgctgcttca 840 ggtaatcata cgaacctctt ttggatcatg caatactgta cagaaagttt tttcattttc 900 cttccaattg tttcttctgg cagggaactt tcattggacc ccagaggatg ttgctaagtc 960 gattctttgc atgatgatgt ctggtccttg tcttactggc tatacacagg tctggtttta 1020 cacaacaaaa agctgacttg ttcttattct agtgcatttg cttggtgcta caataaccta 1080 gacttgtcga tttccagaca atcaacgact ggtatgatag agatatcgac gcaattaatg 1140 agccatatcg tccaattcca tctggagcaa tatcagagcc agaggtaact gagacagaac 1200 attgtgagct tttatctctt ttgtgattct gatttctcct tactccttaa aatgcaggtt 1260 attacacaag tctgggtgct attattggga ggtcttggta ttgctggaat attagatgtg 1320 tgggtaagtt ggcccttctg acattaacta gtacagttaa agggcacatc agatttgcta 1380 aaatcttccc ttatcaggca gggcatacca ctcccactgt cttctatctt gctttgggag 1440 gatcattgct atcttatata tactctgctc cacctcttaa ggtaagtttt attcctaact 1500 tccactctct agtgataaga cactccatcc aagttttgga gttttgaata tcgatatctg 1560 aactgatctc attgcagcta aaacaaaatg gatgggttgg aaattttgca cttggagcaa 1620 gctatattag tttgccatgg taagatatct cgtgtatcaa taatatatgg cgttgttctc 1680 atctcattga tttgtttctt gctcacttga ctgataggtg ggctggccaa gcattgtttg 1740 gcactcttac gccagatgtt gttgttctaa cactcttgta cagcatagct ggggtactct 1800 tttggcaaac cttttatgtt gcttttttcg ttatctgttg taatatgctc ttgcttcatg 1860 ttgtaccttt gtgataatgc agttaggaat agccattgtt aacgacttca aaagtgttga 1920 aggagataga gcattaggac ttcagtctct cccagtagct tttggcaccg aaactgcaaa 1980 atggatatgc gttggtgcta tagacattac tcagctttct gttgccggta tgtactatcc 2040 actgtttttg tgcagctgtg gcttctattt cttttccttg atcttatcaa ctggatattc 2100 accaatggta aagcacaaat taatgaagct gaatcaacaa aggcaaaaca taaaagtaca 2160 ttctaatgaa atgagctaat gaagaggagg catctacttt tatgtttcat tagtgtgatt 2220 gatggatttt catttcatgc ttctaaaaca agtattttca acagtgtcat gaaataacag 2280 aacttatatc ttcatttgta cttttactag tggatgagtt acacaatcat tgttatagaa 2340 ccaaatcaaa ggtagagatc atcattagta tatgtctatt ttggttgcag gatatctatt 2400 agcatctggg aaaccttatt atgcgttggc gttggttgct ttgatcattc ctcagattgt 2460 gttccaggta aagacgttaa cagtctcaca ttataattaa tcaaattctt gtcactcgtc 2520 tgattgctac actcgcttct ataaactgca gtttaaatac tttctcaagg accctgtcaa 2580 atacgacgtc aagtaccagg taagtcaact tagtacacat gtttgtgttc ttttgaaata 2640 tctttgagag gtctcttaat cagaagttgc ttgaaacact catcttgatt acaggcaagc 2700 gcgcagccat tcttggtgct cggaatattt gtaacggcat tagcatcgca acactgaaaa 2760 aggcgtattt tgatggggtt ttgtcgaaag cagaggtgtt gacacatcaa atgtgggcaa 2820 gtgatggcat caactagttt aaaagatttt gtaaaatgta tgtaccgtta ttactagaaa 2880 caactcctgt tgtatcaatt tagcaaaacg gctgagaaat tgtaattgat gttaccgtat 2940 ttgcgctcca tttttgcatt tcctgctcat atcgaggatt ggggtttatg ttagttctgt 3000 cacttctctg ctttcagaat gtttttgttt tctgtagtgg attttaacta ttttc 3055 <210> 4 <211> 1917 <212> DNA <213> Arabidopsis thaliana <400> 4 gttacacgcg taggctagtg gaaaagccca ccacacataa cacacacaca tcttcttcct 60 ctctttatct tcttcttctc tgtttctcta agaagataga acaatcccaa gattctcctc 120 ttatttgatc aaccatgtcg aatgtcttgc tatccccaaa cggctacgta tttgcatcgc 180 ctaaacctct tggcagattc atcaattcaa agtccggcgg acggaaactt ttcttctccg 240 tcgttagagc ttcttccgat gatgctgatt gtaatgccga agaatgtgct cccgagaagg 300 aggtactcaa tcccacctct gtatttgttt cattctccgc tcaattttgt ttctttaaca 360 aaacccatga tgataatttt tttgcaattg ggttgttcaa cattgctggg aaatatgagt 420 tggttactta gaagatttag tttcggaatc tccaaaatta gctaattagt gaaactggat 480 tataaagtgg ggatttttac aattgaaatg atgtcattga tgaggtttgg tgggtgaaac 540 gcaacaggta ggaacagtga gtatggaatg gttagctgga gagaagacga aagtggtggg 600 aacatttcct cctcggaagc cgcgtggttg gactggctat gttgagaaag atactgctgg 660 tcagactaat gtttactcca ttgaggttcg ttcctcttta tttctccatc tatctatcca 720 ctccactcat ctctactttt attagtttag gttataccta agtaagtttt agtatcttta 780 gggaagcaca ggcactcctt ttaggctcat agtgtttgga gggatactgg tttttagttg 840 agagaaaaag cttcaaactt tgccgaggga acaggttctt atgattctgt gataggcatt 900 agaatgtagt ggatgcataa caacaagatg agattagggg tatttgacca gtggcttgtt 960 tgtgagcctt ttggctttca gggagttagg agatctcttt aaccaatttt caatatacat 1020 ttggctcttt ctttgagttt gatcgttgct tcaaggataa caaagaaaac taggctttcg 1080 atctgatcag ttttactgtt atattttgtc tcgtttgaaa ttaacttttt tttggctttt 1140 cttgtgtgtg tgtgtgtttg ttccataatt ctgtcatcga tctcgacctc catctccaag 1200 aaatatgcat tagcttgcaa caacatgtca tgtaaacccc gcatcttctt gagatggggt 1260 tcttgatcta attcgattgg ctttttctta tcgactcaat tccattggct ttttttctaa 1320 agaatagcgt ttcttgtaac tttttcagcc tgcagtatat gttgcagaga gcgcgattag 1380 ctcaggtact gcaggatcat cggctgatgg ggctgagaac acagcagcaa tcgtagcagg 1440 cattgctctt atcgcagtcg ctgcagcttc ctctatactc ctccaagtcg ggaaagatgc 1500 cccaactcga cccaaagcag tggattacag tggaccgtct cttagctact acatcaacaa 1560 gttcaagcct tctgaaattg ttcaaccttc taccccctcc gtcacagaag ccccaccggt 1620 ggccgagcta gaaacttcac tgccagaaac tccatcggtg gctcagcaag aaacctcact 1680 gccggaaact atggctagtg aggctcagcc agaggcgtct tctgttccaa caacaagtag 1740 tacctcttag gggcttagcc taggattata tctttgtgta aaaatgagat gaagcatata 1800 ccaagattat gcattctgtt aatattcgga tatacctata tgtatgtata cactctgtca 1860 ctcttctctt tgagtgaatg attatatcaa atctcaaatc ctattatttt tgctttt 1917 <210> 5 <211> 2089 <212> DNA <213> Arabidopsis thaliana <400> 5 ctccgcctta tcttgttgac tatctctgta ctacttacga gcgcaagtga gagagtaaca 60 gagaaacgta gagatatagc aaaatggctg cgacgcttac tggatcaggg attgctctag 120 ggttttcgtg ctccgcaaag ttctctaaga gagcttcttc gtcgtccaac cgtcgctgca 180 tcaagatgtc ggtttcggta gaggagaaga cgaagaaatt cactcttcag aaatctgagg 240 aagctttcaa tgctgcgaag gttaggcttt tcaatttcca tgaattcagt caataagaaa 300 tgtttcttca gattgattac gccttgatgt tctgttgctt tggttttgtg ggatttgatc 360 atatttttgg ttaaagtgat tatctttgtt acccttcaag gaacattgct aacaaaataa 420 gattacttga gatagaatca taatctgaga taaagttttt gtttttattg gtttcattct 480 gttctgatta ttgaaattgg tttggttcct ttgtgtagaa cttaatgcct ggaggtgtga 540 actcacctgt acgtgctttc aaatctgttg gtggacaacc agtggtgatg gattctgcaa 600 agggctcacg aataagagac attgatggaa atgaatacat tgactatgtt ggatcttggg 660 gacctgctat aattggtcat gctgatgatg aggtttgttt ctttccagtt ttgatgattt 720 ttagatagct gaaagtttag tagttgatac ggtgatgtgt ctagtgcagg ttcttgctgc 780 tttggctgag acaatgaaga aaggaacaag ctttggtgct ccttgtctct tagagaatgt 840 tcttgctgag atggtgattt cagctgttcc aagtattgaa atggttcggt ttgttaactc 900 cggtacagag gcatgtatgg gtgtgctacg tcttgctcgt gccttcacag ggaaacaaaa 960 gttcatcaag tttgaaggtt gttatcatgg tcatgcaaac tctttccttg tcaaagcagg 1020 tagtggtgta gctactttgg gtctacctga ctcgcctggg gtccccaaag cagctacttc 1080 agatacttta acagctccat acaatgatat tgctgctgtt gagaagcttt ttgaggcaaa 1140 caaaggagag attgctgcca tcattcttga acctgttgtt ggtaactcgg gttttattac 1200 acctaaacca gagttcattg agggaatacg ccggatcact aaagacaatg gtgctcttct 1260 tattttcgat gaagtcatga ctggttttcg tttagcctat ggtggagctc aagaatactt 1320 tggaatcaca cctgacttaa caactcttgg gaaaatcatc ggtggtggtc ttcctgtggg 1380 agcatacggt ggaagaagag acatcatgga aatggtaaag ctagagttgt taacaattga 1440 accgaataat cttttgaatt tgctgcaaga gattgagaaa gtcctgatcc tttatcactc 1500 atgtcttttt gcaggttgca cccgcaggac cgatgtatca agctggtacg ctaagtggta 1560 atccgttggc tatgacagcg ggtatacaca cgctgaagcg gttaagtcag ccagggacat 1620 atgaatactt agacaagatc acaaaagagc ttacaaatgg gatactagaa gccgggaaga 1680 aaaccgggca tgcgatgtgt ggcggttaca taagcgggat gttcggtttc ttcttcacgg 1740 aaggacccgt ctatgatttc tcggatgcaa agaagagtga tacagagaag tttggaaagt 1800 ttttcagagg aatgttggaa gaaggtgtct acttggcacc ttctcaattc gaggccggtt 1860 ttactagctt ggctcacact tcagaagaca tccaattcac tatcgcagcg gccgagaagg 1920 ttctaagtcg gctctaaaat gtctctggaa gcttccaggg aaccatgaat gtatttgaaa 1980 ttttttgatg tagagaaaat tagattcaat tgagagcaag caagcaatct cacaaaattt 2040 aactgtggta agaaaagtca aaatgtctat gaagatttgt tgacttttt 2089 <210> 6 <211> 1082 <212> DNA <213> Arabidopsis thaliana <400> 6 cacttgattc tctcttcctt catcaatcaa tctcgtctcc tggaaaacat tagggagcct 60 ctcagatcct caagaaaacc ctaaatcaaa agtcgagatc gatggcgtcc ttcgctcgcc 120 gtgccatgag cttagctcag atcccatctg ctcgtatttc ctctggagcc gcttctattg 180 gtcagcgtcg ttgcctcgcc ggcgccgctg gtaaattctc tctccctctt ccttctcttt 240 tcgatattta cgattttttt cggtatcaat tttggtggcg tatagcttat ttcgcacatt 300 ttatgtctaa tttgtgtttt cacgaatttt agatgaacca aatgttacaa ctttgtgaag 360 attttgtata tcattggcgt tgtcaggttc caaattgaat cactgtatac aaaaaattga 420 gtttttgagc attttgtgga tttgtctgtg tactgtgcag atcaccatgg atctaccaag 480 gttgacttct ggaaacagcc cacgaaccca ggaaactgga aagaggagca tgtatgtatc 540 aatcatgtgt ttgtgacact gtgtttttgt taatgaaagc tgtgattttt tgttgaattg 600 attccttctg ttacctgacc aatctgcagt ttgtgcttat ttctctgtct ggctggggct 660 tacttttcta cagtggatac aagctcgcca ctgggggaaa aaaagaggta cttactcttg 720 ctcttcagct cttgctcttc ttccattgcc tagaggcttc gggttccttc ccttatacat 780 agtttttttt gcagacgcct gtggagagta cacaatgagt ttcattgtca ccaggaagac 840 cttttatgga gagacctctc attctcatgc actttgtttt cccttttagc agattgtgac 900 tggtcttgtg tttcattttg aaaataattt gcatcacttt tgagatctga tgatgtctgc 960 aatggataaa ttcttgacag ttttgtgttt cataatatcc tatgtttgaa acagacagga 1020 tttaagcaaa tcattgtctc actacgtatg ttgatatatg ctttcgagct atttccttcc 1080 at 1082

Claims

A method of increasing the chlorophyll content of chlorella, comprising increasing the expression of the gene of SEQ ID NO: 2 in chlorella.