KR102228332B1 - Sweet potatoes fermented products having high-content beta glucan and Food composition for inhanced immunity containing the same - Google Patents

Sweet potatoes fermented products having high-content beta glucan and Food composition for inhanced immunity containing the same Download PDF

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KR102228332B1
KR102228332B1 KR1020200110760A KR20200110760A KR102228332B1 KR 102228332 B1 KR102228332 B1 KR 102228332B1 KR 1020200110760 A KR1020200110760 A KR 1020200110760A KR 20200110760 A KR20200110760 A KR 20200110760A KR 102228332 B1 KR102228332 B1 KR 102228332B1
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sweet potato
glucan
beta
fermented
content
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KR1020200110760A
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이상종
이혜현
이승협
김미희
추샛별
김민정
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농업회사법인 주식회사 엘바이오텍
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/10Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
    • A23L19/105Sweet potatoes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L19/00Products from fruits or vegetables; Preparation or treatment thereof
    • A23L19/01Instant products; Powders; Flakes; Granules
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30Artificial sweetening agents
    • A23L27/33Artificial sweetening agents containing sugars or derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/065Microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/135Bacteria or derivatives thereof, e.g. probiotics
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/30Foods, ingredients or supplements having a functional effect on health
    • A23V2200/324Foods, ingredients or supplements having a functional effect on health having an effect on the immune system
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2250/00Food ingredients
    • A23V2250/50Polysaccharides, gums
    • A23V2250/502Gums
    • A23V2250/5034Beta-Glucan
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Preparation Of Fruits And Vegetables (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The present invention relates to a sweet potato fermented product containing a high content of beta-glucan and an immune-improving functional food composition containing the same. The sweet potato fermented product containing a high content of beta-glucan of the present invention is prepared by fermenting steamed sweet potato powder, allulose oligosaccharide, and lactic acid bacteria culture solution. According to the present invention, the sweet potato fermented product containing a high content of beta-glucan is prepared by applying a specific fermentation process that can significantly increase the beta-glucan content of existing sweet potato varieties, and an immune-improving functional food composition containing the same is provided.

Description

고함량 베타글루칸을 포함하는 고구마 발효물 및 이를 함유하는 면역개선 기능성 식품 조성물{Sweet potatoes fermented products having high-content beta glucan and Food composition for inhanced immunity containing the same}Sweet potatoes fermented products having high-content beta glucan and Food composition for inhanced immunity containing the same}

본 발명은 고함량 베타글루칸을 포함하는 고구마 발효물 및 이를 함유하는 면역개선 기능성 식품조성물에 관한 것으로서, 보다 상세하게는 베타글루칸 함량을 현저히 상승시킬 수 있는 특정 발효공정을 적용하여 고함량 베타글루칸을 포함하는 고구마 발효물을 제조하고, 이를 함유한 면역개선 기능성 식품조성물에 관한 것이다.The present invention relates to a fermented sweet potato containing a high content of beta-glucan and an immune-improving functional food composition containing the same, and more specifically, a high content of beta-glucan by applying a specific fermentation process capable of significantly increasing the content of beta-glucan. It relates to a functional food composition for improving immunity to prepare a fermented sweet potato containing it and contain it.

고구마는 단위면적당 수확량이 많고 열악한 환경조건에서도 잘 견디며 다방면으로 이용되어 경제성이 높은 작물이다. Sweet potatoes have a high yield per unit area, withstand poor environmental conditions, and are used in a variety of ways, making them a highly economical crop.

고구마의 가치는 전분과 같은 영양성분도 중요하지만 열량이 일반 식량작물에 비하여 낮은 편인데다, 비타민, 미네랄, 식이섬유, 베타-카로틴, 안토시아닌과 같은 생리활성을 가진 기능성 성분이 풍부하다는 데에도 큰 의미가 있다.The value of sweet potatoes is also important in that nutrients such as starch are also important, but their calories are lower than that of general food crops, and that they are rich in functional ingredients with physiological activities such as vitamins, minerals, dietary fiber, beta-carotene, and anthocyanins. have.

고구마는 괴근(塊根, 덩이뿌리, storage roots)을 그대로 식용으로 하거나 쪄먹는 식용고구마 외에도 당면이나 제빵 제과와 같이 전분, 분말 등을 이용한 가공식품이나 고구마 추출음료, 천연색소, 주정, 바이오플라스틱 등 다양한 용도로 이용되고 있다. 고구마를 이용한 식품분야의 연구로 민속주, 묵, 당면, 통조림, 음료, 면류, 제과제빵, 송편, 설기, 양갱, 빵소, 젤리, 고물, 요구르트, 고추장 등이 다양하게 연구된 바 있다. Sweet potato is a variety of processed foods and sweet potato extracts, natural pigments, alcohols, bioplastics, etc., in addition to edible sweet potatoes that are eaten or steamed as they are, such as vermicelli or baking confectionery. It is being used for a purpose. As a research on the food field using sweet potatoes, various studies have been conducted on folk liquor, jelly, vermicelli, canned food, beverages, noodles, confectionery and bakery, songpyeon, seolgi, yokan, baker's, jelly, junk food, yogurt, and red pepper paste.

최근 식생활 패턴의 변화에 따라 다양한 형태의 제과 및 제빵류가 소비되고 있으며, 여기에 여러 가지 건강기능성 소재를 접목시킨 연구들이 증가하고 있다. In recent years, various types of confectionery and bakery are consumed according to changes in dietary patterns, and studies incorporating various health functional materials are increasing.

특히, 고구마는 품종에 따라 독특한 특성을 갖고 있으며, 최근에는 고구마의 기능성 가공 이용성을 향상시킨 새로운 품종의 고구마가 육종되고 있다. 고구마를 과육색에 의해 구분하면 일반고구마와 자색 및 주황색을 띈 유색고구마로 나누며 이들 유색의 색소 성분은 다양한 페놀성 물질을 함유하고 있고 이러한 페놀성 물질은 식물체에 특수한 색깔을 부여하고, 떫은 맛, 쓴맛과 같은 고유한 맛에 관계하며 항산화성, 항균성, 항암성 등의 생리활성을 갖는 것으로 확인되고 있다.In particular, sweet potatoes have unique characteristics depending on the variety, and recently, a new variety of sweet potatoes has been bred to improve the functional processing availability of sweet potatoes. If sweet potatoes are classified by flesh color, they are divided into ordinary sweet potatoes and colored sweet potatoes with purple and orange colors. These colored pigments contain various phenolic substances, and these phenolic substances give a special color to the plant and give it a bitter taste, It is related to intrinsic taste such as bitter taste and has been confirmed to have physiological activities such as antioxidant, antibacterial, and anticancer properties.

그 중 주황고구마와 자색고구마는 그의 기능성이 황색과 자색의 색소에 있어 이들 색소의 이용과 식품개발에는 색소의 특성에 관한 연구가 필요하다. 특히 새로운 식품소재 및 천연 식용색소자원으로 기대되는 주황고구마는 보통고구마의 carotene 함량이 0.02 ㎎/100 g 이하인데 비하여 46~202 ㎎ 으로 대단히 높고 대부분 β-carotene으로 되어있는 것으로 알려져 있다. 그러나, 면역개선효능을 나타내는 베타글루칸의 성분에 대해서는 알려진 게 없는 바, 면역개선효과를 나타낼 수 있는 베타글루칸의 성분을 함유하는 다양한 가공품 개발과 함께 저장 및 이용에 관한 지속적인 연구를 통해 식량자원으로서의 고구마의 효용가치를 높여 소비용도를 확대시키는 것이 필요하다.Among them, orange and purple sweet potatoes have their functionality in yellow and purple pigments. Therefore, research on the characteristics of pigments is required for the use of these pigments and food development. In particular, orange sweet potato, which is expected as a new food material and natural food coloring resource, is known to be very high at 46~202 mg, compared to the carotene content of less than 0.02 mg/100 g, and mostly composed of β-carotene. However, since there is no known component of beta-glucan that exhibits immune-improving effects, sweet potatoes as food resources through continuous research on storage and use along with the development of various processed products containing beta-glucan components that can exhibit immune-improving effects. It is necessary to expand the use of consumption by raising the utility value of the company.

1. 한국 등록특허공보 제10-1846277호 '고구마 요거트 및 이의 제조방법'1. Korean Patent Publication No. 10-1846277'Sweet potato yogurt and its manufacturing method' 2. 한국 등록특허공보 제10-1192033호 '자색고구마 함유 요구르트를 제조하는 방법 및 이에 의해 제조된 자색고구마 함유 요구르트'2. Korean Patent Publication No. 10-1192033'Method of manufacturing yoghurt containing purple sweet potatoes and yoghurt containing purple sweet potatoes'

본 발명의 목적은 베타글루칸 함량을 현저히 상승시킬 수 있는 특정 발효공정을 적용하여 고함량 베타글루칸을 포함하는 고구마 발효물을 제조하고, 이를 함유한 면역개선 기능성 식품조성물을 제공하는 데 있다.An object of the present invention is to prepare a fermented sweet potato containing a high content of beta-glucan by applying a specific fermentation process that can significantly increase the content of beta-glucan, and to provide a functional food composition for improving immunity containing the same.

상기와 같은 목적을 달성하기 위한 본 발명의 고함량 베타글루칸을 포함하는 고구마 발효물은, 찐 고구마 분말, 알룰로오스 올리고당, 유산균 배양액을 넣고 발효하여 제조되는 것이 특징이며, 상기 찐 고구마 분말은 베타글루칸 성분을 갖는 것이 특징이다.The fermented sweet potato containing a high content of beta-glucan of the present invention for achieving the above object is characterized in that it is prepared by fermentation by adding steamed sweet potato powder, allulose oligosaccharide, and lactic acid bacteria culture solution, and the steamed sweet potato powder is beta It is characterized by having a glucan component.

상기 유산균은 서열번호 1로 표현되는 염기서열을 갖는 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) KCTC12357BP 균주인 것이 특징이다. The lactic acid bacteria are characterized by being a Lactobacillus parafarraginis KCTC12357BP strain having a nucleotide sequence represented by SEQ ID NO: 1.

또 다른 본 발명인 면역개선 기능성 식품 조성물은, 상기 고구마 발효물을 포함하는 것이 특징이다.Another aspect of the present invention is an immune-improving functional food composition comprising the fermented sweet potato.

본 발명이 이루고자 하는 기술적 과제들은 이상에서 언급한 기술적 과제들로 제한되지 않으며, 언급되지 않는 다른 기술적 과제들은 아래의 기재로부터 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자에게 명확하게 이해될 수 있을 것이다.The technical problems to be achieved by the present invention are not limited to the technical problems mentioned above, and other technical problems that are not mentioned can be clearly understood by those of ordinary skill in the technical field to which the present invention belongs from the following description. There will be.

본 발명에 의해, 고함량 베타글루칸을 포함하는 고구마 발효물이 제공됨과 동시에 이를 함유하는 면역개선 기능성 식품조성물이 제공된다.By the present invention, a fermented sweet potato containing a high content of beta-glucan is provided, and at the same time, an immune-improving functional food composition containing the same is provided.

도 1은 본 발명에서 사용된 찐 주황고구마 분말의 베타글루칸 함량을 측정한 시험성적서이다.
도 2는 본 발명의 실시예1에 따른 고구마 발효물의 베타글루칸 함량을 측정한 시험성적서이다.
1 is a test report measuring the beta-glucan content of steamed orange sweet potato powder used in the present invention.
2 is a test report measuring the beta-glucan content of fermented sweet potato according to Example 1 of the present invention.

이하, 첨부된 도면을 참조하여 본 발명의 바람직한 실시예 및 실험예를 상세하게 설명하며, 본 발명의 요지를 불필요하게 흐릴 수 있는 공지 기능 및 구성에 대한 상세한 설명은 생략한다.Hereinafter, preferred embodiments and experimental examples of the present invention will be described in detail with reference to the accompanying drawings, and detailed descriptions of known functions and configurations that may unnecessarily obscure the subject matter of the present invention will be omitted.

본 발명은 기존 주황고구마 품종이 갖고 있는 베타글루칸 함량을 현저히 상승시킬 수 있는 특정 발효공정을 적용하여 고함량 베타글루칸을 포함하는 고구마 발효물을 제조하고, 이를 함유한 면역개선 기능성 식품조성물에 관한 것으로써, 보다 상세하게는 상기 고구마 발효물은 찐 고구마 분말, 알룰로오스 올리고당, 유산균 배양액을 넣고 발효하여 제조되는 것이 특징이다.The present invention relates to an immune-improving functional food composition comprising a fermented sweet potato containing a high content of beta-glucan by applying a specific fermentation process that can significantly increase the beta-glucan content of the existing orange sweet potato varieties. In more detail, the fermented sweet potato is characterized in that it is prepared by fermenting steamed sweet potato powder, allulose oligosaccharide, and lactic acid bacteria culture solution.

이하, 본 발명의 고구마 발효물의 일 구현예들로서, 포함될 수 있는 성분들 및 그 제조방법을 살피면 다음과 같다.Hereinafter, as embodiments of the fermented sweet potato of the present invention, the ingredients that may be included and a method of manufacturing the same are as follows.

1. 찐 고구마 분말1. Steamed Sweet Potato Powder

본 발명에서는 베타글루칸 함량을 갖고 있는 고구마 품종을 적용하며, 특히 유산균 배양액의 배양을 용이하게 하도록 하기 위해 보다 바람직하게 찐 고구마 분말을 적용한다. In the present invention, a sweet potato variety having a beta-glucan content is applied, and in particular, steamed sweet potato powder is more preferably applied to facilitate cultivation of the lactic acid bacteria culture solution.

설명하면, 국내 생산되고 있는 고구마 품종으로는 자색고구마, 주황고구마, 호박고구마, 밤고구마 등 여러 품종의 고구마가 생산되고 있다. 그 중 주황고구마는 다른 고구마 품종에 비해 다량의 여러 기능성 성분을 함유하고 있다고 보고되어 있다.In other words, several varieties of sweet potatoes such as purple sweet potatoes, orange sweet potatoes, pumpkin sweet potatoes, and chestnut sweet potatoes are produced as domestic sweet potato varieties. Among them, orange sweet potatoes are reported to contain a large amount of various functional ingredients compared to other sweet potato varieties.

다시말해, 하기 표 1에 나타나 있듯이 국내 생산되고 있는 고구마 품종들에 대하여 기능성 성분을 분석한 결과, 주황고구마에서 전체적인 기능성 성분들이 높게 나타났으며, 본 발명의 발명자들은 표 1에 제시된 여러 기능성 성분 외에 도 1에 나타나 바와 같이 다른 품종에서는 확인되지 않던 베타글루칸 성분이 5.1%(w/w)정도 함유하고 있음을 확인하였다.In other words, as a result of analyzing the functional ingredients for the sweet potato varieties produced in Korea as shown in Table 1 below, the overall functional ingredients were high in the orange sweet potato, and the inventors of the present invention in addition to the various functional ingredients shown in Table 1. As shown in FIG. 1, it was confirmed that the beta-glucan component, which was not identified in other varieties, was contained about 5.1% (w/w).

품종kind 루테인
(mg/100g)
Lutein
(mg/100g)
베타카로틴
(mg/00g)
Beta carotene
(mg/00g)
안토시아닌
(mg/100g)
Anthocyanin
(mg/100g)
비타민C
(mg/100g)
Vitamin C
(mg/100g)
베타글루칸
(%)
Beta Glucan
(%)
자색고구마
(단자미)
Purple sweet potato
(Terminal tail)
42.842.8 54.354.3 208.9208.9 52.0052.00 XX
주황고구마
(풍원미)
Orange Sweet Potato
(Pungwonmi)
35.435.4 133.6133.6 224.9224.9 89.2589.25 5.15.1
호박고구마
(호감미)
Pumpkin Sweet Potato
(Like sweetness)
29.129.1 88.388.3 378.5378.5 44.0044.00 XX
밤고구마
(진율미)
Chestnut sweet potato
(Jinyulmi)
37.137.1 154.0154.0 111.8111.8 37.7637.76 XX

이에, 본 발명에서는 베타글루칸 5~10%(w/w)를 함유하고 있는 고구마 품종하는 것을 특징으로, 보다 바람직하게는 5~10%(w/w)를 함유하고 있는 주황고구마( Ipomoea batatas L.) 풍원미 품종을 적용하는 것이 좋다.Accordingly, in the present invention, a sweet potato variety containing 5 to 10% (w/w) of beta-glucan is characterized, and more preferably, an orange sweet potato containing 5 to 10% (w/w) ( Ipomoea batatas L .) It is better to apply the Pungwonmi variety.

또한, 상기 고구마는 그대로 분말로 사용하는 것보다 찐 고구마 분말을 적용할 경우 유산균의 발효능이 더욱 우수하게 작용하게 된다. In addition, the fermentation ability of lactic acid bacteria is more excellent when steamed sweet potato powder is applied than the sweet potato is used as a powder as it is.

다시말해, 통상 고구마 분말 대비 찐고구마의 전분함량, 수분흡수지수 및 수분용해지수, 그리고 식이섬유의 함량이 찌는 과정을 통해 증가하는 것이 보고되어 있다(출처: 열풍건조 및 분무건조 공정을 이용한 생 고구마와 찐 고구마 분말제조 및 물리화학적 품질특성, 한국키틴키토산학회, 2017)In other words, it has been reported that the starch content, moisture absorption and water dissolution index, and dietary fiber content of steamed sweet potatoes compared to ordinary sweet potato powder increase through the steaming process (Source: Raw sweet potatoes using hot air drying and spray drying processes). And Steamed Sweet Potato Powder Preparation and Physical and Chemical Quality Characteristics, Korean Chitin and Chitosan Society, 2017)

따라서, 생고구마 분말을 적용할 경우에는 발효과정시 유산균이 충분히 배양하기에 수분함량이 맞지 않아 유산균배양능이 떨어지게 되는 바, 본 발명이 목적하는 고함량의 베타글루칸의 함량을 얻기 어렵게 된다. 이에 반면, 찐고구마 분말의 경우에는 별도의 수분을 추가하지 않더라도 충분히 유산균 배양능을 최적화 할 수 있게 해주게 되는 바, 본 발명에서는 찐 고구마 분말을 적용하는 것이 바람직하다.Therefore, when the raw sweet potato powder is applied, the water content is not appropriate for the lactic acid bacteria to sufficiently cultivate during the fermentation process, so that the lactic acid bacteria cultivation capacity is degraded, making it difficult to obtain the high content of beta-glucan for the purpose of the present invention. On the other hand, in the case of steamed sweet potato powder, it is possible to sufficiently optimize lactic acid bacteria cultivation capacity even without adding additional moisture. In the present invention, it is preferable to apply steamed sweet potato powder.

2. 알룰로오스 올리고당2. Allulose Oligosaccharide

올리고당은 포도당, 과장, 시럽 등의 영양감미료라고 불리우는 물질들의 대체제로 많이 사용되고 있는 것으로써, 그 종류로는 프락토 올리고당, 대두 올리고당, 말토 올리고당, 갈락토 올리고당, 알룰로오스 올리고당이 있다. Oligosaccharides are widely used as a substitute for substances called nutrient sweeteners such as glucose, hyperbole, and syrup, and their types include fructo-oligosaccharide, soybean oligosaccharide, malto-oligosaccharide, galacto-oligosaccharide, and allulose oligosaccharide.

그 중 알룰로오스 올리고당은 다른 감미료보다 자연 상태의 설탕과 가까운 깔끔한 단맛을 내면서 칼로리는 설탕(g당 4kcal)의 약 5%에 불과하여 거의 무칼로리 감미료로 적용되는 물질로 알려져 있다.Among them, allulose oligosaccharide is known to be a substance that is applied as an almost calorie-free sweetener because it produces a clean sweet taste that is closer to natural sugar than other sweeteners, and its calories are only about 5% of sugar (4 kcal per gram).

이에 본 발명은 최종 목적인 식품 조성물 소재로써 상기 고구마 발효물을 사용하는 것으로써 칼로리는 거의 변화없으면서 유산균의 배양능을 높일 수 있도록 도와주는 소재로 올리고당 중에서도 알룰로오스 올리고당을 사용하는 것이 가장 적합하다.Accordingly, the present invention is to use the fermented sweet potato as a food composition material that is the final object, and it is most suitable to use allulose oligosaccharide among oligosaccharides as a material that helps to increase the cultivation ability of lactic acid bacteria while almost no change in calories.

3. 유산균 배양액3. Lactobacillus culture medium

다양한 유용미생물 중에서도 상기 찐 고구마 분말의 유효성분 즉, 베타글루칸의 함량을 최대한 높일 수 있는 유용미생물들을 적용한 것으로서, 유산균 배양액을 적용한다. Among various useful microorganisms, useful microorganisms capable of increasing the content of the active ingredient of the steamed sweet potato powder, that is, beta-glucan as much as possible, are applied, and a lactic acid bacteria culture solution is applied.

설명하면, 상기 유산균 배양액은 장내에 정착하여 병원성 세균이 소화관 상피에 부착하는 것을 방해하여 질병발생을 막아주며, 유산균에 의해 생성된 항생물질이 설사를 일으키는 병원성 미생물이나 장내 유해균을 죽이거나 증식을 억제해 줌과 동시에 찐 고구마 분말의 유효성분 중 베타글루칸 함량을 최대한 상승시켜주는 역할을 한다. In other words, the lactic acid bacteria culture medium settles in the intestine and prevents disease by preventing pathogenic bacteria from attaching to the epithelium of the digestive tract, and antibiotics produced by lactic acid bacteria kill pathogenic microorganisms that cause diarrhea or harmful bacteria in the intestine or inhibit proliferation. At the same time, it plays a role in increasing the beta-glucan content of the active ingredients of steamed sweet potato powder as much as possible.

이때, 상기 유산균으로는 장내 생존율 높고 기능성성분의 함량을 극대화시켜줄수 있도록 호기성 유산균인 락토바실러스 속 균주들을 적용하는 것이 좋으며, 보다 바람직하게는 본 발명의 발명자가 보유하고 있는 한국생명공학연구원에서 2013년 01월 23일에 기탁된 서열번호 1로 표현되는 염기서열을 갖는 락토바실러스 파라파라지니스 균주 KCTC 12357BP 균주를 적용하는 것이 가장 좋다.At this time, as the lactic acid bacteria, it is recommended to apply strains of the genus Lactobacillus, which are aerobic lactic acid bacteria, so as to maximize the content of functional ingredients with high intestinal viability, and more preferably, in 2013 from the Korea Research Institute of Bioscience and Biotechnology owned by the inventor of the present invention. It is best to apply the Lactobacillus paraparaginis strain KCTC 12357BP strain having the nucleotide sequence represented by SEQ ID NO: 1 deposited on January 23.

여기서 상기 배양액은 균주를 배양한 배지 내에 포함되어 있는 모든 물질을 포함하는 물질을 의미할 수 있으며, 예컨대 균주 배양의 결과물인 대사물 또는 분비물을 포함하는 물질 또는 그 파쇄물을 의미할 수 있고 균주 자체도 배양물 내에 포함되어 있을 수 있다. 또한 상기 배양액은 당업계에 통상적으로 이용되는 방법에 따라 배양할 수 있다.Herein, the culture medium may mean a material including all substances contained in the culture medium in which the strain is cultivated, and for example, may mean a substance including a metabolite or secretion product or a lysate thereof, which is a result of culturing the strain, and the strain itself is also It may be contained within the culture. In addition, the culture medium may be cultured according to a method commonly used in the art.

상기 배지로는 통상의 배지가 사용될 수 있으며, 상기 락토바실러스 파라파라지니스 균주 KCTC 12357BP를 MRS 액상배지에 접종한 후, 이를 30~35℃에서 24~72시간 동안 배양하여 1×106 CFU/ml 이상 농도의 미생물 배양액을 준비하는 것이 특징이며, 더욱 바람직하게는 상기 액상배지에 상기 미생물 균주를 접종한 다음 30~35℃에서 24~72시간 동안 0.05~0.4 vvm(vol/vol/min)의 통기량과 교반속도 100~200 rpm(rotary/min), 내압 0.1~1 kgf/㎠으로 배양하며, 이때 배양된 상기 락토바실러스 파라파라지니스 균주 KCTC 12357BP 배양액의 pH는 3.0~5.0 인 것이 적합하다.As the medium, a conventional medium may be used, and after inoculating the Lactobacillus paraparaginis strain KCTC 12357BP into MRS liquid medium, incubating it at 30 to 35° C. for 24 to 72 hours to 1×10 6 CFU/ml It is characterized by preparing a microbial culture solution of an abnormal concentration, and more preferably, after inoculating the microbial strain in the liquid medium, a container of 0.05 to 0.4 vvm (vol/vol/min) for 24 to 72 hours Skills and agitation speed 100 ~ 200 rpm (rotary / min), and the culture at 0.1 ~ 1 kgf / ㎠ internal pressure, at this time, the pH of the cultured Lactobacillus paraparaginis strain KCTC 12357BP culture medium is suitable for 3.0 ~ 5.0.

(고함량 베타글루칸을 포함하는 고구마 발효물의 제조방법)(Method of manufacturing fermented sweet potato containing high content of beta glucan)

본 발명은 상기와 같이 준비된 찐 고구마 분말, 알룰로오스 올리고당, 유산균 배양액을 함께 넣고 발효하여 고함량의 베타글루칸을 포함하는 고구마 발효물을 제조한다.In the present invention, the steamed sweet potato powder, allulose oligosaccharide, and lactic acid bacteria culture solution prepared as described above are added together and fermented to prepare a fermented sweet potato containing a high content of beta glucan.

이때, 상기 찐 고구마 분말은 고구마를 80~120℃의 증기의 열로 40~60분동안 익힌 후, 이를 건조하여 제조하는 것이 특징으로, 건조방식은 열풍건조 또는 분무건조 등 어떠한 건조방식을 적용하여도 가능하다.At this time, the steamed sweet potato powder is characterized in that the sweet potato is cooked for 40 to 60 minutes by heat of steam at 80 to 120°C, and then dried. The drying method may be any drying method such as hot air drying or spray drying. It is possible.

이렇게 제조된 찐고구마 분말은 고구마 발효물의 전체중량을 기준으로 80~ 91중량%로 함유되는 것을 특징으로, 80 중량% 미만으로 함유될 경우에는 본 발명이 목적하는 고함량 베타글루칸을 함유하는 고구마 발효물을 얻기 어려우며, 91 중량%를 초과할 경우에는 보다 상승된 베타글루칸 함량을 나타내는 것이 아니며, 오히려 발효가 제대로 이루어지지 않게 되는 문제가 발생된다.The steamed sweet potato powder thus prepared is characterized in that it is contained in an amount of 80 to 91% by weight based on the total weight of the fermented sweet potato, and if it is contained in less than 80% by weight, the sweet potato fermentation containing the high content beta-glucan for the purpose of the present invention It is difficult to obtain water, and when it exceeds 91% by weight, it does not indicate an elevated beta-glucan content, but rather, a problem occurs in that fermentation is not properly performed.

또한, 상기 알룰로오스 올리고당은 고구마 발효물의 전체중량을 기준으로 0.5~2중량%로 함유되는 것을 특징으로, 0.5 중량% 미만으로 함유될 경우에는 유산균 발효능이 촉진되지 않으며, 2 중량%를 초과할 경우에는 다른 재료들과의 배합비율이 맞지 않아 이 역시 소비자들의 선호도를 떨어뜨릴 우려가 있게 된다.In addition, the allulose oligosaccharide is characterized in that it is contained in an amount of 0.5 to 2% by weight based on the total weight of the fermented sweet potato, and if it is contained in less than 0.5% by weight, the lactic acid bacteria fermentation ability is not promoted, and exceeds 2% by weight. In this case, there is a concern that the mixing ratio with other ingredients will not match and this will also reduce the preference of consumers.

또한, 상기 유산균 배양액은 고구마 발효물의 전체중량을 기준으로 8~19중량%로 함유되는 것을 특징으로, 8 중량%미만일 경우에는 그 함량이 너무 적어 베타글루칸 상승능이 떨어져 본 발명이 목적하는 효능을 얻지 못하게 되며, 19 중량%를 초과할 경우에는 다른 재료들과의 배합비율이 맞지 않아 이 역시 본 발명이 목적하는 효능을 얻지 못할 우려가 있게 된다.In addition, the lactic acid bacteria culture medium is characterized in that it is contained in an amount of 8 to 19% by weight based on the total weight of the fermented sweet potato, and if it is less than 8% by weight, the content is too small and the beta-glucan synergistic ability decreases, so that the desired effect of the present invention is not obtained. If it exceeds 19% by weight, the blending ratio with other materials is not correct, so there is a fear that the desired efficacy of the present invention may not be obtained.

또한, 상기 발효는 30~35℃에서 4~5일동안 발효하는 것을 특징으로, 상기 발효조건을 벗어날 경우 과발효로 인한 이취가 발생되어 오히려 소비자들에게 거부감을 줄 우려가 있게 되며, 발효시간이 부족할 경우에는 베타글루칸 함량의 상승효과가 떨어지게 되는 바, 남녀노소 누구나 즐기면서 베타글루칸 함량이 높은 식품 조성물을 제공하기 위해서는 상기 발효조건을 지켜주는 것이 가장 좋다.In addition, the fermentation is characterized by fermentation at 30 to 35°C for 4 to 5 days, and when the fermentation conditions are out of the above fermentation conditions, an off-flavor is generated due to over-fermentation, and there is a fear of giving consumers a sense of rejection, and the fermentation time is reduced. If insufficient, the synergistic effect of the beta-glucan content decreases. In order to provide a food composition having a high beta-glucan content while enjoying everyone, it is best to observe the fermentation conditions.

이렇게 제조된 상기 최종 본 발명인 고구마 발효물은 도 2에 나타나 있듯이 베타글루칸의 함량이 8.33%(w/w)로써 고함량을 갖게 되는 바, 이를 면역개선 기능성 식품 조성물의 소재로 사용할 수 있게 되며, 사용 목적에 적합하게 액상, 분말상 등으로 제조될 수 있으며, 특히 분말상의 경우에는 고구마 발효물을 감압 농축하여 분말화시킬 수 있다.As shown in FIG. 2, the final fermented sweet potato product of the present invention thus prepared has a high content of 8.33% (w/w) of beta glucan, which can be used as a material for an immune-improving functional food composition, It may be prepared in a liquid or powdered form suitable for the purpose of use. In particular, in the case of a powdery form, the fermented sweet potato may be concentrated under reduced pressure to be powdered.

다시 말해, 본 발명의 고구마 발효물은 상기 설명된 고함량의 베타글루칸 성분 및 다양한 기능성 성분이 다량으로 함유되어 있으므로, 예를 들어, 대장암, 유방암, 전립선암, 간암, 췌장암 또는 위암 등의 각종 악성 종양, 세균성 질환, 진균 질환, 고혈압, 우울증, 경련, 바이러스성 질환, 통증, 위장 장애, 심장 기능 저하, 흉통, 감기, 당뇨, 설사, 이질, 부종, 신경통, 신경쇄약, 류마티스성 관절염, 노화, 각종 염증 질환, 불면증, 천식 등의 면역개선을 필요로 하는 면역개선 기능성 식품 조성물의 소재로 사용될 수 있으며 이는, 상기 질환들의 예방 또는 개선에 효과적인 식품 및 음료 등에 다양하게 이용될 수 있다. In other words, the fermented sweet potato of the present invention contains a large amount of the beta-glucan component and various functional components of the above-described high content, for example, various types of colon cancer, breast cancer, prostate cancer, liver cancer, pancreatic cancer, or stomach cancer. Malignant tumors, bacterial diseases, fungal diseases, high blood pressure, depression, convulsions, viral diseases, pain, gastrointestinal disorders, decreased heart function, chest pain, cold, diabetes, diarrhea, dysentery, edema, neuralgia, nerve breakdown, rheumatoid arthritis, aging , Various inflammatory diseases, insomnia, asthma, etc. It can be used as a material for an immune-improving functional food composition that requires immunity improvement, which can be used in various ways such as foods and beverages effective in preventing or improving the above diseases.

본 발명의 유효 성분을 포함하는 식품으로는, 예를 들어, 각종 식품류, 음료, 껌, 차, 비타민 복합제, 건강보조 식품류, 요플레, 떡 등이 있고, 분말, 과립, 정제, 캡슐 또는 음료인 형태로 사용할 수 있다. 본 발명의 유효성분은 일반적으로 전체 식품 중량의 0.01 내지 15중량%로 가할 수 있으며, 건강음료 조성물은 100ml를 기준으로 0.02 내지 10g, 바람직하게는 0.3 내지 1g의 비율로 가할 수 있다.Foods containing the active ingredient of the present invention include, for example, various foods, beverages, chewing gum, tea, vitamin complexes, health supplements, yoplait, rice cakes, etc., and powders, granules, tablets, capsules, or beverages. Can be used as. In general, the active ingredient of the present invention may be added in an amount of 0.01 to 15% by weight of the total food weight, and the health beverage composition may be added in an amount of 0.02 to 10g, preferably 0.3 to 1g, based on 100ml.

본 발명의 건강 기능 식품은 지시된 비율로 필수 성분으로서 상기 유효성분을 함유하는 것 외에 식품학적으로 허용 가능한 식품보조 첨가제, 예컨대, 천연 탄수화물 및 다양한 향미제 등을 추가 성분으로서 함유할 수 있다.The health functional food of the present invention may contain, as an additional ingredient, food supplementary additives, such as natural carbohydrates and various flavoring agents, in addition to containing the active ingredient as an essential ingredient in the indicated ratio.

상기 천연 탄수화물의 예로는 포도당, 과당 등의 단당류, 말토오스, 수크로오스 등의 이당류 및 덱스트린, 시클로덱스트린 등의 다당류와 같은 통상적인 당 및 자일리톨, 소르비톨, 에리쓰리톨 등의 당알코올이 있다.Examples of the natural carbohydrates include monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and conventional sugars such as polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol.

상기 향미제로는 타우마틴, 레바우디오시드 A, 글리시르히진, 사카린, 아스파르탐 등을 사용할 수 있다 상기 향미제의 비율은 본 발명의 건강 기능 식품 100ml당 일반적으로 약 1 내지 20g, 바람직하게는 약 5 내지 12g을 사용한다.Taumatin, rebaudioside A, glycyrrhizin, saccharin, aspartame, etc. may be used as the flavoring agent. The ratio of the flavoring agent is generally about 1 to 20 g per 100 ml of the health functional food of the present invention, preferably Uses about 5 to 12 g.

상기 외에 본 발명의 건강 기능 식품은 여러 가지 영양제, 비타민, 광물, 합성 풍미제 및 천연 풍미제 등의 풍미제, 착색제 및 중진제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산음료에 사용되는 탄산화제 등을 함유할 수 있다. 그 밖에 본 발명의 건강 기능 식품은 천연 과일 주스 및 과일 주스 음료 및 야채 음료 등의 제조를 위한 과육을 함유할 수도 있다.In addition to the above, the health functional food of the present invention includes a variety of nutrients, vitamins, minerals, synthetic flavors and natural flavors, and other flavoring agents, coloring agents and thickeners, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloids. It may contain thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In addition, the health functional food of the present invention may contain pulp for the production of natural fruit juices, fruit juice beverages, and vegetable beverages.

이러한 성분은 독립적으로 또는 조합하여 사용할 수 있다. 이러한 첨가제의 비율은 본 발명의 상기 유효성분 100중량부 당 0.01 내지 약 20중량부의 범위에서 선택되는 것이 일반적이다.These components may be used independently or in combination. The ratio of these additives is generally selected in the range of 0.01 to about 20 parts by weight per 100 parts by weight of the active ingredient of the present invention.

이하에서는 실시예 및 실험예를 들어 본 발명에 관하여 더욱 상세하게 설명할 것이나, 이들 실시예 및 실험예는 단지 설명의 목적을 위한 것으로 본 발명의 보호 범위를 제한하고자 하는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to Examples and Experimental Examples, but these Examples and Experimental Examples are for illustrative purposes only and are not intended to limit the protection scope of the present invention.

<실시예 1> 본 발명인 고구마 발효물1 제조<Example 1> Preparation of fermented sweet potato 1 of the present invention

도 1에 도시되어 있듯이 베타글루칸 함량이 5.1%(w/w) 함유되어 있는 주황고구마(풍원미, Ipomoea batatas L.) 150kg을 100℃의 수증기로 50분동안 익혀 찐고구마를 제조한 후, 이를 열풍건조하여 찐고구마 분말을 제조하였다. As shown in FIG. 1, 150 kg of orange sweet potatoes (Pungwonmi, Ipomoea batatas L.) containing 5.1% (w/w) beta-glucan were cooked for 50 minutes with steam at 100° C. to prepare steamed sweet potatoes, and then this was done. Steamed sweet potato powder was prepared by hot air drying.

그 다음 상기 찐고구마 분말 100kg에 알룰로오스 올리고당 1.5 kg, 서열번호 1로 표현되는 염기서열을 갖는 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) KCTC12357BP 배양액 15ℓ(MRS 배지, 1×107 CFU/ml)를 넣고 35℃에서 4일동안 발효한 후, 이를 열풍건조하여 도 2에 나타나 있는 고함량 베타글루칸을 포함하는 고구마 발효물1을 제조하였다.Then, to 100 kg of the steamed sweet potato powder, 1.5 kg of allulose oligosaccharide , Lactobacillus parafarraginis having a nucleotide sequence represented by SEQ ID NO: 1, 15 L of KCTC12357BP culture solution (MRS medium, 1×10 7 CFU/ml) was added. Then, after fermentation at 35° C. for 4 days, it was hot-air dried to prepare a fermented sweet potato 1 containing a high content beta glucan shown in FIG.

<비교예 1> 고구마 발효물2 제조<Comparative Example 1> Preparation of fermented sweet potato 2

상기 실시예 1과 같은 방법으로 제조하되, 상기 찐고구마 분말 대신 생고구마 분말을 적용하여 고구마 발효물2를 제조하였다.It was prepared in the same manner as in Example 1, but the fermented sweet potato 2 was prepared by applying fresh sweet potato powder instead of the steamed sweet potato powder.

<비교예 2> 고구마 발효물3 제조<Comparative Example 2> Preparation of fermented sweet potato 3

상기 실시예 1과 같은 방법으로 제조하되, 상기 락토바실러스 파라파라지니스 균주 대신 바실러스 서브틸리스 균주를 적용하여 고구마 발효물3을 제조하였다.It was prepared in the same manner as in Example 1, but the fermented sweet potato 3 was prepared by applying a Bacillus subtilis strain instead of the Lactobacillus paraparaginis strain.

<비교예 3> 고구마 발효물4 제조<Comparative Example 3> Preparation of fermented sweet potato 4

상기 실시예 1과 같은 방법으로 제조하되, 상기 락토바실러스 파라파라지니스 균주 대신 락토바실러스 파라카제이 균주를 적용하여 고구마 발효물4를 제조하였다.It was prepared in the same manner as in Example 1, but the fermented sweet potato 4 was prepared by applying the Lactobacillus paracasei strain instead of the Lactobacillus paraparaginis strain.

<비교예 4> 고구마 발효물5 제조<Comparative Example 4> Preparation of fermented sweet potato 5

상기 실시예 1과 같은 방법으로 제조하되, 상기 유산균 배양액 15ℓ대신 5ℓ를 넣고 발효하여 고구마 발효물5를 제조하였다.It was prepared in the same manner as in Example 1, but 5 ℓ of the lactic acid bacteria culture broth was added and fermented to prepare a fermented sweet potato 5.

<실험예 1> 베타 글루칸 함량 확인<Experimental Example 1> Confirmation of beta glucan content

1. 실험방법1. Experimental method

상기 실시예 1 및 비교예 1 내지 4의 고구마 발효물들에 대하여 베타글루칸 함량을 측정하였다. 측정은 Megazyme kit인 Mixed-Linkage β-glucan kit를 사용하였으며, 총 글루칸 함량(total-glucan)에서 알파글루칸 함량(α-glucan)을 빼는 방식으로 베타글루칸 함량을 측정하였다.The beta-glucan content was measured for the fermented sweet potato products of Example 1 and Comparative Examples 1 to 4. The measurement was performed using the Megazyme kit Mixed-Linkage β-glucan kit, and the beta-glucan content was measured by subtracting the alpha-glucan content (α-glucan) from the total glucan content (total-glucan).

다시 말해, 우선 각 고구마 발효물 100mg씩에 15ml HCl을 각각 넣고 45분간 방치한 후 10ml의 물을 첨가하여 2시간 반응시키고 10ml의 2N KOH를 첨가한 다음, 200mM sodium acetate 완충액으로 100ml로 증액한 후 원심분리하여 상등액 0.1ml를 용액[(exo-1,3 β-glucanase)와 β-glucosidase의 혼합액]과 혼합하고 1시간 반응시켰다.In other words, first, 15 ml of HCl was added to each 100 mg of each fermented sweet potato, left for 45 minutes, 10 ml of water was added to react for 2 hours, 10 ml of 2N KOH was added, and then the solution was increased to 100 ml with 200 mM sodium acetate buffer. After centrifugation, 0.1 ml of the supernatant was mixed with a solution [mixture of (exo-1,3 β-glucanase) and β-glucosidase] and reacted for 1 hour.

그 다음으로, 3ml GOPOD 혼합액(glucose reagent + glucose oxidase, peroxidase, 4- aminoantipyridine)을 넣고 20분간 반응시킨 후 510nm에서 흡광도를 측정하여 총 글루칸 함량을 확인하였다.Next, 3ml GOPOD mixture (glucose reagent + glucose oxidase, peroxidase, 4-aminoantipyridine) was added and reacted for 20 minutes, and the absorbance was measured at 510 nm to determine the total glucan content.

알파글루칸 함량의 측정방법으로는 먼저 각 고구마 발효물 100mg씩에 2ml KOH를 각각 넣고, 혼합한 후 20분간 ice water bath에서 균질화 하였다. 다음으로 8ml 12M sodium acetate buffer를 넣은 후 용액(50% glycerol + amyloglucosidase 1,630 U/ml + invertase 500 U/ml solution)을 0,2ml 혼합하여 30분 반응 후 원심분리하였다. 이후 상등액 0.1ml에 GOPOD 혼합액을 첨가하여 20분 반응 후 510nm에서 흡광도를 측정하여 알파글루칸 함량을 확인하였다.As a method of measuring the alpha-glucan content, 2ml KOH was first added to 100mg of each fermented sweet potato, mixed, and homogenized in an ice water bath for 20 minutes. Next, after adding 8ml 12M sodium acetate buffer, 0,2ml of the solution (50% glycerol + amyloglucosidase 1,630 U/ml + invertase 500 U/ml solution) was mixed, reacted for 30 minutes, and then centrifuged. Thereafter, a GOPOD mixture was added to 0.1 ml of the supernatant, reacted for 20 minutes, and the absorbance was measured at 510 nm to confirm the alpha glucan content.

2. 실험결과2. Experiment result

상기 실험결과, 하기 표 2와 같이 나타났다.As a result of the above experiment, it was shown in Table 2 below.

베타글루칸 함량(w/w %)Beta glucan content (w/w %) 대조군(찐 주황고구마)Control (Steamed Orange Sweet Potato) 5.15.1 실시예 1Example 1 8.38.3 비교예 1Comparative Example 1 6.16.1 비교예 2Comparative Example 2 5.35.3 비교예 3Comparative Example 3 7.07.0 비교예 4Comparative Example 4 6.56.5

상기 표 2에 나타나 있듯이 본 발명인 실시예 1의 경우에는 찐 주황고구마 분말 보다 베타글루칸의 함량이 상승됨을 확인하였다. 이에 반면, 실시예 1과 같은 발효공정을 거치더라도 찐 주황고구마가 아닌 생고구마 분말을 적용한 비교예 1의 경우에는 생각보다 베타글루칸의 함량이 상승되지 않았음을 확인하였다. As shown in Table 2, in the case of Example 1 of the present invention, it was confirmed that the content of beta-glucan was increased compared to the steamed orange sweet potato powder. On the other hand, in the case of Comparative Example 1 in which fresh sweet potato powder was applied instead of steamed orange sweet potato even through the same fermentation process as in Example 1, it was confirmed that the content of beta-glucan was not increased.

또한, 동종균주로 같은 유산균 속에 해당되는 균을 적용한 비교예 3의 경우에는 베타글루칸의 함량이 대조군 보다 높게 나타나긴 하였으나, 실시예 1보다는 낫게 나타났으며, 유산균이 아닌 다른 바실러스 균을 적용한 비교예2의 경우에는 발효과정을 통해 상승된 베타글루칸 함량을 얻지 못함을 확인하였다. In addition, in the case of Comparative Example 3 in which the bacteria corresponding to the same lactic acid bacteria were applied as the homologous strain, the content of beta-glucan was higher than that of the control group, but it was better than Example 1, and a comparative example in which Bacillus bacteria other than the lactic acid bacteria were applied. In case 2, it was confirmed that an elevated beta-glucan content was not obtained through the fermentation process.

유산균 배양액의 함량이 다소 낮게 첨가된 비교예 4의 경우 역시 최적의 발효과정이 이루어지지 않게 되는 바, 베타글루칸의 함량이 상승되는 정도가 적음을 확인하였다.In the case of Comparative Example 4 in which the content of the lactic acid bacteria culture medium was added slightly lower, it was confirmed that the optimal fermentation process was not performed, and the degree of increase in the content of beta-glucan was small.

이와 같이 본 발명에 의해, 고함량 베타글루칸을 포함하는 고구마 발효물을 제조하고, 이를 함유한 면역개선 기능성 식품조성물을 제제화하여 소비자에게 제공할 수 있게 됨을 알 수 있다.As described above, according to the present invention, it can be seen that a fermented sweet potato containing a high content of beta-glucan can be prepared, and an immune-improving functional food composition containing the same can be formulated and provided to consumers.

이하, 여러 공정에 따른 제제들을 나타내면 다음과 같다.Hereinafter, formulations according to various processes are as follows.

(제제예 1: 면역기능 개선 고구마 요플레 제조)(Formulation Example 1: Immune Function Improvement Sweet Potato Yoplait Manufacturing)

저온 살균해둔 우유 100ml에 유산균 1~5g을 넣고 혼합하여 유산균을 접종한 다음, 32℃에서 24시간동안 발효한 후, 유청을 제거하여 그릭요거트를 제조하엿다.Lactobacillus 1-5g was added to 100 ml of pasteurized milk, mixed and inoculated with lactic acid bacteria, fermented at 32°C for 24 hours, and then whey was removed to prepare Greek yogurt.

그 다음 상기 그릭요거트와 상기 실시예 1에서 제조한 고구마 발효물을 혼합하되 2 : 0.1~0.5 중량비로 혼합하여 면역개선 기능성 식품조성물을 제조하고, 이를 면역기능 개선 고구마 요플레로 명명하였다.Then, the Greek yogurt and the fermented sweet potato prepared in Example 1 were mixed, but mixed at a weight ratio of 2: 0.1 to 0.5 to prepare an immune-improving functional food composition, which was named as immune function-improving sweet potato yopla.

(제제예 2: 면역기능 개선 떡 제조)(Formulation Example 2: Preparation of rice cake for improving immune function)

고구마 발효물 100 중량부에 대하여, 맵쌀 200~250 중량부, 찹쌀 5~30 중량부, 소금 0.05~0.5 중량부, 설탕 0.05~0.5 중량부를 넣고 혼합하여 면역개선 기능성 식품조성물을 제조한 후, 이를 90~100℃에서 15~30분동안 쪄서 면역기능 개선 떡을 제조하였다.With respect to 100 parts by weight of fermented sweet potato, 200 to 250 parts by weight of spicy rice, 5 to 30 parts by weight of glutinous rice, 0.05 to 0.5 parts by weight of salt, 0.05 to 0.5 parts by weight of sugar are added and mixed to prepare an immune-improving functional food composition. Steamed at 90-100° C. for 15-30 minutes to prepare a rice cake with improved immune function.

상기의 본 발명은 바람직.한 실시예 및 실험예를 중심으로 살펴보았으며, 본 발명이 속하는 기술분야에서 통상의 지식을 가진 자는 본 발명의 본질적 기술 범위 내에서 상기 본 발명의 상세한 설명과 다른 형태의 실시예들을 구현할 수 있을 것이다. 여기서 본 발명의 본질적 기술범위는 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다. The present invention described above is preferred. It has been looked at around one embodiment and an experimental example, and those of ordinary skill in the art to which the present invention pertains are in a form different from the detailed description of the present invention within the essential technical scope of the present invention. It will be possible to implement embodiments of. Here, the essential technical scope of the present invention is indicated in the claims, and all differences within the scope equivalent thereto should be construed as being included in the present invention.

한국생명공학연구원Korea Research Institute of Bioscience and Biotechnology KCTC12357BPKCTC12357BP 2013012320130123

<110> Lbio technology co.,ltd <120> Sweet potatoes fermented products having high-content beta glucan and Food composition for inhanced immunity containing the same <130> Lbio-04 <150> KR 10-2019-0132249 <151> 2019-10-23 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1137 <212> DNA <213> Lactobacillus sp. <400> 1 acatgcaagt cgaacgaggc aaccataatg aagttaggtg cttgcattta actgatttaa 60 cattgagacg agtggcgaac tggtgagtaa cacgtgggta acctgccctg aagtggggga 120 taacacttgg aaacaggtgc taataccgca taacaacgaa aaccacatgg ttttcgtttg 180 aaagatggct tcggctgtca cttttggatg gacccgcggc gtattagctt gttggtgagg 240 taacggctca ccaaggccat gatacgtagc cgacctgaga gggtaatcgg ccacattggg 300 actgagacac ggcccaaact cctacgggag gcagcagtag ggaatcttcc acaatggacg 360 aaagtctgat ggagcaacgc cgcgtgagtg atgaagggtt tcggctcgta aaactctgtt 420 gttggagaag aacaggtgat agagtaactg ttatcatctt gacggtatcc aaccagaaag 480 ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg ttgtccggat 540 ttattgggcg taaagcgagc gcaggcggtt ttttaggtct gatgtgaaag ccttcggctt 600 aaccggagaa gggcatcgga aaccgggaga cttgagtgca gaagaggaca gtggaactcc 660 atgtgtagcg gtgaaatgcg tagatatatg gaagaacacc agtggcgaag gcggctgtct 720 ggtctgtaac tgacgctgag gctcgaaagc atgggtagcg aacaggatta gataccctgg 780 tagtccatgc cgtaaacgat gagtgctaag tgttggaggg tttccgccct tcagtgctgc 840 agctaacgca ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa 900 ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgatgcta cgcgaagaac 960 cttaccaggt cttgacatct tctgctaacc taagagatta ggcgttccct tcggggacgg 1020 aatgacaggt ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg 1080 caacgagcgc aacccttatt gtcagttgcc agcatttagt tgggcactct ggcgaga 1137 <110> Lbio technology co.,ltd <120> Sweet potatoes fermented products having high-content beta glucan and Food composition for inhanced immunity containing the same <130> Lbio-04 <150> KR 10-2019-0132249 <151> 2019-10-23 <160> 1 <170> KoPatentIn 3.0 <210> 1 <211> 1137 <212> DNA <213> Lactobacillus sp. <400> 1 acatgcaagt cgaacgaggc aaccataatg aagttaggtg cttgcattta actgatttaa 60 cattgagacg agtggcgaac tggtgagtaa cacgtgggta acctgccctg aagtggggga 120 taacacttgg aaacaggtgc taataccgca taacaacgaa aaccacatgg ttttcgtttg 180 aaagatggct tcggctgtca cttttggatg gacccgcggc gtattagctt gttggtgagg 240 taacggctca ccaaggccat gatacgtagc cgacctgaga gggtaatcgg ccacattggg 300 actgagacac ggcccaaact cctacgggag gcagcagtag ggaatcttcc acaatggacg 360 aaagtctgat ggagcaacgc cgcgtgagtg atgaagggtt tcggctcgta aaactctgtt 420 gttggagaag aacaggtgat agagtaactg ttatcatctt gacggtatcc aaccagaaag 480 ccacggctaa ctacgtgcca gcagccgcgg taatacgtag gtggcaagcg ttgtccggat 540 ttattgggcg taaagcgagc gcaggcggtt ttttaggtct gatgtgaaag ccttcggctt 600 aaccggagaa gggcatcgga aaccgggaga cttgagtgca gaagaggaca gtggaactcc 660 atgtgtagcg gtgaaatgcg tagatatatg gaagaacacc agtggcgaag gcggctgtct 720 ggtctgtaac tgacgctgag gctcgaaagc atgggtagcg aacaggatta gataccctgg 780 tagtccatgc cgtaaacgat gagtgctaag tgttggaggg tttccgccct tcagtgctgc 840 agctaacgca ttaagcactc cgcctgggga gtacgaccgc aaggttgaaa ctcaaaggaa 900 ttgacggggg cccgcacaag cggtggagca tgtggtttaa ttcgatgcta cgcgaagaac 960 cttaccaggt cttgacatct tctgctaacc taagagatta ggcgttccct tcggggacgg 1020 aatgacaggt ggtgcatggt tgtcgtcagc tcgtgtcgtg agatgttggg ttaagtcccg 1080 caacgagcgc aacccttatt gtcagttgcc agcatttagt tgggcactct ggcgaga 1137

Claims (5)

베타글루칸 성분을 갖는 고구마를 증기의 열로 익힌 후, 이를 건조하여 베타글루칸 성분을 갖는 찐고구마 분말을 제조하는 제1단계 및,
상기 베타글루칸 성분을 갖는 찐 고구마 분말 80~ 91중량%, 알룰로오스 올리고당 0.5~2중량%, 서열번호 1로 표현되는 염기서열을 갖는 락토바실러스 파라파라지니스(Lactobacillus parafarraginis) KCTC12357BP 균주를 포함하는 배양액 8~19중량%을 혼합한 후, 이를 30~35℃에서 4~5일동안 발효하여 고함량 베타글루칸을 포함하는 고구마 발효물을 제조하는 제2단계;를 포함하는,
고함량 베타글루칸을 포함하는 고구마 발효물의 제조방법.
A first step of preparing a steamed sweet potato powder having a beta-glucan component by cooking the sweet potato having a beta-glucan component by heat of steam, and drying it, and,
A culture medium containing 80 to 91% by weight of steamed sweet potato powder having the beta-glucan component, 0.5 to 2% by weight of allulose oligosaccharide, and Lactobacillus parafarraginis KCTC12357BP strain having a nucleotide sequence represented by SEQ ID NO: 1. After mixing 8 to 19% by weight, the second step of fermenting it at 30 to 35 °C for 4 to 5 days to prepare a fermented sweet potato containing a high content of beta glucan; including,
A method for producing a fermented sweet potato containing a high content of beta-glucan.
삭제delete 삭제delete 제1항의 제조방법에 의해 제조되어,
8%(w/w)이상의 베타글루칸을 포함하는 것이 특징인,
고함량 베타글루칸을 포함하는 고구마 발효물.
It is manufactured by the manufacturing method of claim 1,
Characterized by containing more than 8% (w / w) of beta glucan,
Fermented sweet potato containing high content of beta glucan.
제4항의 고구마 발효물을 포함하는,
면역개선 기능성 식품 조성물.
Including the fermented sweet potato of claim 4,
Immunity-improving functional food composition.
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