KR102217731B1 - Antioxidant composition comprising acorn extract and use of the same - Google Patents

Abstract

The present invention is an antioxidant composition comprising an acorn extract, wherein the acorn is a fruit of Rubra oak and/or Great oak, and a food composition, functional food composition, cosmetic composition and pharmaceutical composition comprising the antioxidant composition and the antioxidant composition It is about.

Description

Antioxidant composition comprising acorn extract and use of the same}

The present invention relates to an antioxidant composition comprising an acorn extract and a use thereof, and more particularly, as an antioxidant composition comprising an acorn extract, wherein the acorn is a fruit of a Rubra oak and/or a giant oak, and It relates to a food composition, a functional food composition, a cosmetic composition and a pharmaceutical composition comprising the antioxidant composition.

Oak trees that open acorns are widely distributed in the mountainous regions of Korea, and about 28 species are known to be distributed, including the native species of Korea, such as oak, red oak, red oak, oyster oak, oak, and Japanese oak. Typical edible acorns are Quercus serrata Murray ) fruit (acorn) and oak ( Quercus autissima) Carruther ) is the fruit of the tree.

The chemical composition of acorns is composed of about 70% starch and contains about 6 to 9% of tannin, and the tannins are naturally astringent and bitter, so when acorns are used for food, they are removed. .

Acorns are used after peeling the shell and separating the nut part inside. Mix the acorn powder with a large amount of water and brew it, then settle the sediment and filter it to separate the fiber and starch components in the acorn powder. In this process, not only fiber and starch are separated, but also the tannins in the starch escape through diffusion.

Non-patent literature on the antioxidant activity of acorns discloses component analysis and antioxidant activity evaluation of acorn powder (Non-Patent Document 1), antioxidant activity and quality characteristics of cookies added with acorn powder (Non-Patent Document 2), etc. , The type of acorn used in the prior art is a fruit derived from an oak tree or a sol oak tree, or it is difficult to determine the type in detail.

On the other hand, patent documents related to acorns include an acorn extract having anti-inflammatory activity and a pharmacological composition containing the extract (Patent Document 1), a food composition for manufacturing ready-made foods using acorns (Patent Document 2), and acorn extract. A method for producing fermented grain wine using (Patent Document 3) and the like are disclosed.

However, the patent documents do not disclose any kind of oak tree that the acorn is derived from even if the use of the acorn extract is not related to antioxidant or antioxidant uses.

In addition, the acorns of the Rubra oak and/or Great oak that the present inventors studied are fruits that are not currently used for edible use, so they were the first to discover a composition with high antioxidant power, and food compositions, functional food compositions, and cosmetic compositions containing them As a pharmaceutical composition and a new use value is sufficient.

Korean Patent Application Publication No. 10-2012-0051928 Republic of Korea Patent Publication No. 10-2017-0119101 Republic of Korea Patent Publication No. 10-2017-0095064

Rep. Inst. Health & Environ. 16：46-52, 2005 KOREAN J. FOOD COOKERY SCI. Vol. 29, No. 2 April, 2013

The present inventors conducted research to solve the problems of the prior art as described above, and 8 kinds of acorns that are widely planted in Korea, that is, Quercus acutissima ) , Quercus variabilis), oak (Quercus serrata), go oak (Quercus aliena Blume ) , Quercus mongolica Fisch), upon closing trees (Quercus glauca), the great oak (Quercus palustris ) , Quercus rubra ( Quercus rubra ), the total phenol content was measured using acorns, antioxidant power was evaluated, and a comparative analysis was performed between species, and the present invention was completed.

Accordingly, an object of the present invention is to provide a composition for antioxidant comprising acorn extract derived from a specific oak tree having excellent phenol content and antioxidant activity.

Another object of the present invention is to provide a use of an antioxidant composition comprising the acorn extract.

In order to achieve the above object, the present invention provides an antioxidant composition comprising an acorn extract, wherein the acorn is one or more fruits selected from the group consisting of Lubra oak and Giant oak.

In addition, the present invention provides an antioxidant composition in which the extract is a water extract, or an alcohol having 1 to 4 carbon atoms, preferably ethanol.

In addition, the present invention provides an antioxidant composition in which the acorn extract is an acorn ethanol extract, and the concentration of the acorn ethanol extract is 0.005 to 0.010 mg/ml.

In addition, the present invention provides an antioxidant food composition comprising the antioxidant composition as an active ingredient.

In addition, the present invention provides an antioxidant health functional food composition comprising the antioxidant composition as an active ingredient.

In addition, the present invention provides an antioxidant cosmetic composition comprising the antioxidant composition as an active ingredient.

In addition, the present invention provides an antioxidant pharmaceutical composition comprising the antioxidant composition as an active ingredient.

The antioxidant composition comprising the extract of the Rubra oak and/or the Great Oak acorn of the present invention has an effect of showing a significantly higher phenol content and antioxidant activity than the acorn extract derived from other types of oaks.

1 shows the measurement range of phenol according to an embodiment of the present invention.
Figure 2 shows the DPPH activity (%) of 8 species of oak acorn ethanol extract.
Figure 3 shows the FRAP antioxidant activity of the ethanol extract of 8 kinds of oak acorns.
Figure 4 shows the TAC antioxidant activity of 8 kinds of oak acorn ethanol extract.
Figure 5 shows the ascorbic acid (ascorbic acid) antioxidant activity of the ethanol extract of acorns of eight oak species.

Hereinafter, the present invention will be described in detail through examples. However, these examples are for illustrative purposes only, and the scope of the present invention is not limited by these examples.

<Example>

1. Analysis of moisture content of publicly available samples

Each seed is an oak tree ( Quercus) collected from the test forest oak variety conservation center managed by the National Institute of Forest Science. acutissima ), Quercus variabilis ), Quercus serrata ), Quercus aliena Blume , Quercus mongolica Fisch ), Quercus glauca ), the great oak ( Quercus palustris ), and the samples of the lubra oak ( Quercus rubra ) were used.

The collected acorns were dried in the shade at room temperature for 7 days, and after drying, they were pulverized into 40 mesh to form a powder, and the results of measuring the moisture content are shown in Table 1 below. The acorn powder pulverized in the above was used directly to prepare the extract.

2. Determination of total phenol content

(One). Insoluble tannin content

The phenolic components of the acorn extract were classified into total phenol, water soluble phenol, non-nitrogen phenol, and tannin phenol and quantified. The method of quantification is presented in Figure 1.

As for the total phenol, an extract obtained by extracting a 0.25 g powder sample with 8 ml of 80% methanol with a shaker for 24 hours was used for analysis.

For the total non-nitrogen phenol, polyvinyl polypyrrolidone was added to a certain amount using the 80% methanol extract, mixed with stirring, reacted at 40° C. for 15 minutes, and then centrifuged each supernatant was used for analysis, The tannic phenol content was calculated as the limiting value of the non-nitrogenic phenol content from the total phenol content.

Each extract and supernatant were reacted with Folin and Ciocalteu's phenol reagent, and the concentration of each phenol was measured at 640 nm. At this time, the comparison was performed using gallic acid as a control.

The total phenol content (unit: mg/g), non-nitrogen phenol content (unit: mg/g), and tannin content (unit: mg/g) are shown in Tables 2 to 4 below in order.

As shown in Table 2, there was a difference in content by tree species, and the content difference was confirmed in the order of Rubra oak> Daewang oak> Japanese oak> Red oak> Jongno oak> oak> Oyster oak> Singal oak.

Remarkable from these results, the highest phenol content was observed in the acorns of Rubra oak and Giant oak among the 8 species used.

In addition, as shown in Table 3 (non-tannic phenol) and Table 4 (tannic phenol), which are the results obtained by dividing the total phenol content into tannic phenol and non-tannic phenol content, all eight types of tannic phenol The content was found to be superior to the non-nitrogen phenol content.

Looking at the previously reported research results, it is expected that acorns consist of non-nitrogen phenol compounds such as gallic acid and tannic compounds combined with carbohydrates and proteins as the main components of acorns.

(2). Water soluble tannin content

As for the total phenol, an extract obtained by extracting a 0.25 g powder sample with 8 ml of water (distilled water) with a shaker for 24 hours was used for analysis.

Using the water extract, polyvinyl polypyrrolidone was added to a certain amount, mixed with stirring, reacted at 40° C. for 15 minutes, and then centrifuged each supernatant was used for analysis.

Each extract and supernatant were reacted with Folin and Ciocalteu's phenol reagent, and the concentration of each phenol was measured at 640 nm. At this time, the comparison was performed using gallic acid as a control.

The total water-soluble tannin content (unit: mg/g) is shown in Table 5 below.

(3). Antioxidant power evaluation

(3-1). Extract extraction yield

For the evaluation of antioxidant power, each acorn powder was immersed in 95% ethanol for 72 hours, and after complete concentration using a concentrator, the extraction yield was calculated. The content of the final extract is shown in Table 6 below.

As shown in Table 6, the yield of the extract was confirmed from a minimum of 5.7% to a maximum of 27.6%. In particular, the yield of the Great Oak and Rubra Oak was significantly higher than that of other species. As a result of this, it is thought that the useful components of Rubra oak and Giant oak, which have high ethanol extract yield, will be higher than other species.

(3-2). DPPH analysis

DPPH (1,1-diphenyl-2-picrylhydrazyl) analysis was measured by the principle of free radical scavenging ability . 100 µl of ethanol extract of each sample was added to 100 µl of 0.15 mM DPPH solution.

After the reaction solution was completely mixed, it was reacted at room temperature for 10 minutes. In order to measure the amount of DPPH, the absorbance was measured at 517 nm using a spectrophotometer (UV-vis spectrophotometer), and the results are shown in Table 7 and FIG. 2 below.

At this time, the antioxidant activity of free radical scavenging ability was calculated by substituting in Equation 1 below.

[Equation 1]

Free radical scavenging ability (%)=[1-(absorbance of sample at 517 nm/absorbance of control at 517 nm)]*100

As shown in Table 7, the activity was high in the order of rubra oak> giant oak> bell oak> white oak> red oak> oak> oyster oak> red oak.

The most important result to be examined here is that even in DPPH analysis, the activity of acorns of Rubra oak and Great oak was measured the highest.

The degree of activity was expressed as EC ₅₀ (half maximal effective concentration; mg/ml) as the concentration of the antioxidant that reduces the DPPH concentration at the beginning of the reaction to 50%, and in the case of the 8 species used in this example, 0.078 mg/ml ~ The EC ₅₀ result was shown at the sample concentration of 0.005 mg/ml.

Although DPPH analysis is the simplest method of measuring antioxidant activity, it has experimental limitations due to the mechanistic differences in hydrogen molecule transfer occurring in the reaction between antioxidants and peroxy radicals.

Therefore, additional FRAP assay, total antioxidant capacity (TAC) assay, and ascorbic acid assay were performed in order to clarify the result of the activity difference between species. Since the evaluation of the activity in all additional experiments is confirmed through the absorption of UV, an absorbance interference problem may occur in the case of a dark sample.

Even in the case of this experiment, there is a difference in degree, but since all 8 species contain their own color, the color of the sample does not interfere with the absorbance measurement, and the sample concentration condition within the DPPH activity range is 0.078 mg/ml ~ 0.005 mg/ml. I did.

(3-3). Ferric iron reducing activity (FRAP) assay

Unlike the method of measuring DPPH radical scavenging ability, the FRAP measurement method is a method of measuring the antioxidant ability by reducing power, and the reducing power was evaluated to the extent that it reduces the Fe ^{3 +} ions of the fractions to Fe ^{2 +} ions.

For the experiment, the Oxiselect ^TM Ferric Reducing Antioxidnat Power Assay Kit (Catalog Number: STA-859) developed by CELL BIOBABS was used. According to the Kit method, each sample and the solution in the kit were reacted at 37° C. for 5 minutes, and the absorbance at 593 nm was measured.

At this time, the degree of activity was based on quantitative data of absorbance of ferric iron (Iron II) used as a standard, and the calibration curve range was set to 2000 μM, 1000 μM, 250 μM, and 125 μM, and the calibration curve result y=0.001 It came out as x+0.3014 (R ² =0.9904).

As a result of the investigation of the same extract, as shown in FIG. 3, the antioxidant effect was confirmed in the order of rubura oak> giant oak> bell oak> sol oak> red oak> oak> oyster oak> red oak.

The difference between the species was confirmed when the concentration of the sample was 0.078 mg/ml, and as a result, FRAP activity was also the highest in the activity of Rubra oak and Giant oak.

(3-4). Total antioxidant capacity assay

The experiment was performed using the Oxiselect ^TM Total Antioxidant Capacity Assay Kit (Catalog Number: STA-360) developed by CELL BIOBABS. Absorbance was measured at 660 nm after 5 minutes of reaction with each sample and solution in the kit according to the Kit method.

A standard curve was prepared using uric acid (C ₅ H ₄ N ₄ O ₃ ) as a standard reagent, and the calibration curve range was set to 2000 μM, 1000 μM, 250 μM, and 125 μM, and the TAC activity was mM uric acid equivalent (uric acid). equivalent). The calibration curve result was y=0.0017x+0.1299 (R ² =0.9985).

The total antioxidant capacity measurement method is a method of evaluating the integrated antioxidant capacity in water-soluble and fat-soluble systems. In the TAC measurement, a result of comparing the activity using uric acid having an oxidation factor removal ability as a standard indicator is shown in FIG. 4.

As shown in FIG. 4, as a result of the TAC measurement, the activity of each species was confirmed in the order of Rubra oak> Great oak> Bell oak> Japanese oak> Red oak> Oak> Oyster oak> Red oak.

This result was confirmed as a result similar to DPPH. In this result, the antioxidant activity of Rubra oak and Giant oak was relatively higher than that of other oak tree species at each concentration.

(3-5). Ascorbic acid assay

For the experiment, the Oxiselect ^TM AScorbic Acid Assay Kit (Catalog Number: STA-860) developed by CELL BIOBABS was used. According to the Kit method, absorbance was measured at 540 nm after 3 minutes of reaction with each sample and solution in the kit.

The activity was compared using ascorbic acid as a standard. The calibration curve range was set to 2μM, 0.5μM, 0.25μM, 0.125μM, and 0.0625μM, and the calibration curve result was y=0.8403x+0.1707 (R ² =0.9984).

Ascorbic acid, also known as vitamin C, is a representative reducing agent. Therefore, it is known as a major standard used as a positive control for the evaluation of antioxidant reducing power.

The results of comparing the activity using ascorbic acid as a standard are shown in Fig.5.As a result of this evaluation, also in this evaluation, in the order of Lubra oak> Great oak> Bell oak> Japanese oak> Japanese oak> oak> Oyster oak> Japanese red oak The activity was measured to be high.

In general, the main substance of the antioxidant effect of oak trees is known as tannin, and in particular, it is known to have a high content of gallic acid. There are few other references regarding the known oak tree acorn extract. In particular, there are no references to the acorn extract in the case of the Great Oak or Lubra Oak.

As described above, the present invention has been described with reference to a preferred embodiment of the present invention, but those of ordinary skill in the art of the present invention can see the present invention within the scope not departing from the spirit and scope of the invention described in the following claims. It will be appreciated that various modifications and changes can be made to the invention.

Since the antioxidant composition containing the extract of the Rubra oak and/or the Great Oak acorn of the present invention has the effect of showing a significantly higher phenol content and antioxidant activity compared to the acorn extract derived from other types of oak, the present invention belongs It can be usefully applied to the field of developing food compositions, functional food compositions, cosmetic compositions and pharmaceutical compositions.

Claims (7)

As an antioxidant composition comprising an acorn extract,
The acorn is at least one kind of fruit selected from the group consisting of rubra oak and giant oak,
The acorn extract is an acorn ethanol extract,
The concentration of the acorn extract is 0.078 mg/ml to 0.005 mg/ml,
The composition is a food, health functional food or cosmetic composition for antioxidant. delete delete An antioxidant food composition comprising the antioxidant composition of claim 1 as an active ingredient. An antioxidant health functional food composition comprising the antioxidant composition of claim 1 as an active ingredient. An antioxidant cosmetic composition comprising the antioxidant composition of claim 1 as an active ingredient. delete

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