KR102196465B1 - Device and method for ecis based cell culture chamber controlling - Google Patents

Abstract

An ECIS-based cell culture chamber control apparatus according to an embodiment of the present application includes a communication unit receiving a control signal for controlling an electrode module and a measurement module from a user terminal, the electrode module applying electrical stimulation to each of the plurality of cell culture chambers, and A module control unit for controlling the measurement module for measuring impedance according to stimulation for each of a plurality of cell culture chambers, and a database unit for recording impedance data of the cell culture chamber measured from the measurement module, wherein the control signal is the It is transmitted to the module control unit through the database unit, and the module control unit changes the electrical stimulation of the electrode module based on the control signal, and measures the change in impedance of the electrode due to cell growth according to the electrical stimulation. It can be measured through.

Description

ECIS-based cell culture chamber control device and method {DEVICE AND METHOD FOR ECIS BASED CELL CULTURE CHAMBER CONTROLLING}

The present application relates to an ECIS-based cell culture chamber control apparatus and method.

Electric cell-substrate impedance spectroscopy (ECIS) is one of a kind of electrical impedance spectroscopy that uses the phenomenon of increased impedance between electrodes as cell mobility increases. When cells are attached and spread to the electrode, the current is physically disturbed and the impedance increases. The increase in impedance can be used to assess cell behavior, response to drugs, and barrier function of cancer cells or stem cells, since cellular parameters such as cell status, cell number and cell viability can be determined.

In measuring impedance, a method of digitally collecting signals and calculating impedance using digital signal processing (DSP) is one of the conventional methods. However, the conventional calculation method not only requires a considerable calculation time, but also has a problem in that the integration degree is low and occupies a lot of space because a digital device is used.

The technology behind the present application is disclosed in Korean Patent Publication No. 10-1023251.

The present application is to solve the above-described problems of the prior art, and it is an object of the present invention to provide an ECIS-based cell culture chamber control apparatus and method that minimizes the degree of integration by implementing the IOT method and improves efficiency in impedance calculation.

However, the technical problems to be achieved by the embodiments of the present application are not limited to the technical problems as described above, and other technical problems may exist.

As a technical means for achieving the above technical problem, the ECIS-based cell culture chamber control apparatus according to an embodiment of the present application includes a communication unit receiving a control signal for controlling an electrode module and a measurement module from a user terminal, and a plurality of cell cultures The electrode module for applying electrical stimulation to each chamber, a module controller for controlling the measurement module for measuring impedance according to the electrical stimulation for each of a plurality of cell culture chambers, and impedance data of the cell culture chamber measured from the measurement module A database unit for recording, wherein the control signal is transmitted to the module control unit through the database unit, and the module control unit changes the electric stimulation of the electrode module based on the control signal, and cell growth according to the electric stimulation A change in the impedance of the electrode due to may be measured through the measurement module.

According to an embodiment of the present application, the module control unit includes an analog front end (AFE) and a single board computer (SBC), the communication unit is connected to the SBC, and the electrode module and the measurement module are connected to the AFE. I can.

According to an embodiment of the present application, the measurement module includes a USB oscilloscope, and the module control unit generates a sine wave based on a function generator of the USB oscilloscope, and the electrode module is configured to generate the plurality of Electrical stimulation can be applied to the cell culture chamber.

According to an exemplary embodiment of the present disclosure, the measurement module may non-invasively measure impedance due to currents flowing through the plurality of cell culture chambers according to the resistance of the preset size.

According to an embodiment of the present application, the control signal may include at least one of a frequency, a channel, a measurement interval, an experiment period, and an experiment name.

According to an exemplary embodiment of the present application, the module controller may measure the impedance based on Equation 1.

According to an exemplary embodiment of the present disclosure, the complex gain may be calculated based on Equation 2.

According to an embodiment of the present application, the module controller may estimate the amplitude and phase of the stimulus signal that minimizes Equation (3).

The ECIS-based cell culture chamber control method according to an embodiment of the present application includes the steps of: (a) receiving a control signal for controlling an electrode module and a measurement module from a user terminal, (b) applying electrical stimulation to each of the plurality of cell culture chambers. Controlling the measurement module for measuring the impedance according to the applied electrode module and the electrical stimulation for each of a plurality of cell culture chambers, and (c) recording the impedance data of the cell culture chamber measured from the measurement module. Including, wherein the step (b), the electrical stimulation of the electrode module is changed based on the control signal, and a change in impedance of the electrode due to cell growth according to the electrical stimulation can be measured through the measurement module. have.

According to an embodiment of the present application, the measurement module includes a USB oscilloscope, and step (b) generates a sine wave based on a function generator of the USB oscilloscope, and the electrode module is Electrical stimulation may be applied to the plurality of cell culture chambers.

According to an exemplary embodiment of the present disclosure, the measurement module may non-invasively measure impedance due to currents flowing through the plurality of cell culture chambers according to the resistance of the preset size.

According to an embodiment of the present application, the control signal may include at least one of a frequency, a channel, a measurement interval, an experiment period, and an experiment name.

According to the exemplary embodiment of the present application, step (b) may measure the impedance based on Equation 4.

According to an exemplary embodiment of the present disclosure, the complex gain may be calculated based on Equation 5.

According to an embodiment of the present application, in step (b), the amplitude and phase of the stimulus signal minimizing Equation 6 may be estimated.

The above-described problem solving means are merely exemplary and should not be construed as limiting the present application. In addition to the above-described exemplary embodiments, additional embodiments may exist in the drawings and detailed description of the invention.

According to the above-described problem solving means of the present application, it is possible to provide an ECIS-based cell culture chamber control apparatus and method that minimizes the degree of integration by implementing the IOT method and improves efficiency in impedance calculation.

1 is a diagram showing the configuration of an ECIS-based cell culture chamber control apparatus according to an embodiment of the present application.
2 is a diagram showing an IOT implementation of the ECIS-based cell culture chamber control apparatus according to an embodiment of the present application.
FIG. 3 is a diagram illustrating an example of a circuit in which a resistor and a capacitor of the ECIS-based cell culture chamber control device according to an embodiment of the present disclosure are dusurfehl in series and emulate the impedance of the chamber.
4 is a view showing an example of a multiplexer connected to the cell culture chamber of the ECIS-based cell culture chamber control apparatus according to an embodiment of the present application.
5 is a view showing a block diagram of a connected culture chamber to explain an impedance calculation method of the ECIS-based cell culture chamber control apparatus according to an embodiment of the present application.
6 is a view showing the flow of the ECIS-based cell culture chamber control method according to an embodiment of the present application.

Hereinafter, exemplary embodiments of the present application will be described in detail with reference to the accompanying drawings so that those of ordinary skill in the art may easily implement the present application. However, the present application may be implemented in various different forms and is not limited to the embodiments described herein. In addition, in the drawings, parts not related to the description are omitted in order to clearly describe the present application, and similar reference numerals are attached to similar parts throughout the specification.

Throughout this specification, when a part is said to be "connected" with another part, this includes not only the case that it is "directly connected", but also the case that it is "electrically connected" with another element interposed therebetween. do.

Throughout this specification, when a member is positioned "on", "upper", "upper", "under", "lower", and "lower" of another member, this means that a member is located on another member. It includes not only the case where they are in contact but also the case where another member exists between the two members.

Throughout the specification of the present application, when a certain part "includes" a certain component, it means that other components may be further included rather than excluding other components unless specifically stated to the contrary.

1 is a view showing the configuration of an ECIS-based cell culture chamber control apparatus according to an embodiment of the present application.

Referring to FIG. 1, the ECIS-based cell culture chamber control apparatus 100 may include a communication unit 110, a module control unit 120, and a database unit 130. The communication unit 110 may receive a control signal for controlling the electrode module and the measurement module from the user terminal. For example, the communication unit 110 may receive a control signal from a user terminal through a network. The network refers to a wired and wireless connection structure capable of exchanging information between respective nodes such as a terminal and a server, and examples of such networks include a 3rd Generation Partnership Project (3GPP) network and a Long Term Evolution (LTE) network. , 5G network, WIMAX (World Interoperability for Microwave Access) network, Internet, LAN (Local Area Network), Wireless LAN (Wireless Local Area Network), WAN (Wide Area Network), PAN (Personal Area Network), Bluetooth (Bluetooth) network, satellite broadcasting network, analog broadcasting network, DMB (Digital Multimedia Broadcasting) network, and the like are included, but are not limited thereto.

In addition, the user terminal is, for example, a smartphone (Smartphone), a smart pad (SmartPad), a tablet PC, and PCS (Personal Communication System), GSM (Global System for Mobile communication), PDC (Personal Digital Cellular), PHS (Personal Handyphone System), PDA (Personal Digital Assistant), IMT (International Mobile Telecommunication)-2000, CDMA (Code Division Multiple Access)-2000, W-CDMA (W-Code Division Multiple Access), Wibro (Wireless Broadband Internet) It can be any kind of wireless communication device.

On the other hand, electric cell-substrate impedance spectroscopy (ECIS) is one of a kind of electrical impedance spectroscopy that uses the phenomenon of increased impedance between electrodes as cell mobility increases. When cells are attached and spread to the electrode, the current is physically disturbed and the impedance increases. The increase in impedance can be used to assess cell behavior, response to drugs, and barrier function of cancer cells or stem cells, since cellular parameters such as cell status, cell number and cell viability can be determined. These cell studies using ECIS aim to ultimately improve the quality of life. For example, cytotoxicity studies can investigate the health effects of workers handling toxic substances. ECIS can be used to investigate the regulation of endothelial permeability leading to edema and loss of organ function leading to systemic vascular leakage due to malfunction. Meanwhile, it has been applied to the relationship between mesenchymal stem cells and oxygen tension in studies on cancer progression and mesenchymal stem cell therapy, and has great potential for human pathology, including cardiovascular disease, neurodegenerative disease, and osteoarthritis.

In ECIS, the impedance can be calculated by measuring the voltage obtained by stimulating the electrode of the cell chamber with a sinusoidal current of several uA. In addition, the complex impedance can be obtained by calculating a component of a received signal that is in-phase and quadrature phase as a stimulus signal. The complex impedance may be calculated by using a conventional analog lock-in amplifier or by digitally collecting two signals and then using digital signal processing (DSP). Meanwhile, a general multipurpose impedance measuring instrument such as a fixed amplifier and a frequency or network analyzer requires a lot of space and is not easy to control. Accordingly, the ECIS-based cell culture chamber control apparatus 100 of the present application may solve the space problem by using a high-performance USB oscilloscope.

The module controller 120 may control the electrode module for applying electrical stimulation to each of the plurality of cell culture chambers and the measurement module for measuring impedance according to the electrical stimulation for each of the plurality of cell culture chambers. Specifically, the module controller 120 may change the electrical stimulation of the electrode module based on the control signal, and measure a change in impedance of the electrode due to cell growth according to the electrical stimulation through the measurement module. In addition, the module control unit 120 may include an analog front end (AFE) and a single board computer (SBC). In this case, the communication unit 110 may be connected to the SBC, and the electrode module and the measurement module may be connected to the AFE. The ECIS-based cell culture chamber control device 100 may be implemented in an IoT format. As the ECIS-based cell culture chamber control device 100 is implemented in an IOT format, the system can be controlled and monitored without a monitor or standard input/output device such as a keyboard/mouse through a web-based user interface. In addition, not only the space of the input/output device is reduced, but careless input due to the ease of access to the input/output device can be prevented. In addition, in the case of an ECIS system that requires a long-term experiment that lasts for several days at intervals of several minutes, the ECIS-based cell culture chamber control apparatus 100 of the present application may be more usefully utilized by being implemented in an IOT format.

2 is a view showing an IOT implementation of the ECIS-based cell culture chamber control apparatus according to an embodiment of the present application.

The measurement module may include a USB oscilloscope. Referring to FIG. 2, the USB oscilloscope may be connected to an AFE, and the AFE may be connected to an SBC through a USB interface. In addition, as the communication unit 110 is connected to the SBC, the SBC may be connected to the network through the communication unit 110.

The module controller 120 may generate a sine wave based on the function generator of the USB oscilloscope. For example, the sine wave may be a 1 Vpp sine file. In addition, the electrode module may apply electrical stimulation to a plurality of cell culture chambers through resistance of a preset size. In addition, the measurement module may non-invasively measure impedance due to currents flowing through a plurality of cell culture chambers according to a resistance of a preset size. The resistance of the preset size may be 1 MΩ by way of example. When an electrical stimulation is applied to any one of the plurality of cell culture chambers by the electrode module, since the current flowing through the chamber is less than 1 μA, non-invasive detection of the measurement module may be possible.

3 is a diagram showing an example of a circuit for emulating the impedance of the chamber in which the resistance and the capacitor of the ECIS-based cell culture chamber control device according to an embodiment of the present application are dusurfehl in series, and FIG. 4 is an embodiment of the present application A diagram showing an example of a multiplexer connected to the cell culture chamber of the ECIS-based cell culture chamber control device according to FIG.

The module controller 120 may determine the amplifier type, the number of samples per period, and parameters of the impedance calculation method. For example, the number of samples per period may play an important role in selecting an oscilloscope. In order to determine the number of samples per cycle, a simulation may be performed through an emulator that emulates the impedance of the culture chamber with a resistor and a capacitor. According to the simulation, as shown in FIG. 3, the impedance of the culture chamber may be connected in series with a resistance of 1k to 8kΩ and a capacitor of 10 nF. FIG. 4 shows an example in which a 1kΩ resistor and a 10nF capacitor are connected in series, and a circuit that emulates the impedance of the chamber is connected to the multiplexer (ie, AFE) of FIG. 3. According to conventional studies, in most studies, the frequency of the stimulation current was about 100 kHz and the culture cell resistance was set within about 50 kΩ. Accordingly, the ECIS-based cell culture chamber control apparatus 100 may set the gain to 100 so that the amplifier does not saturate when measuring a chamber having a maximum impedance with reference to a conventional study. Also, based on the maximum frequency and the selected gain, an amplifier with a gain bandwidth of 10 MHz or more can be selected and utilized, and since the stimulus current is less than 1 μA, an amplifier with a low input offset current and voltage can be selected and utilized.

On the other hand, if the number of samples per cycle is too small, measurement errors due to noise increase, so the amount of noise can be said to be the most important factor in determining the number of samples per cycle. Since the ECIS-based cell culture chamber control apparatus 100 has the worst signal-to-noise ratio among chamber emulators having the lowest impedance, the amount of noise may be determined by measuring a voltage received therefrom.

5 is a view showing a block diagram of a connected culture chamber to explain an impedance calculation method of the ECIS-based cell culture chamber control apparatus according to an embodiment of the present application.

Conventionally, lock-in detection was mainly used for impedance calculation, but an example using a sine fitting method for measuring the DNA concentration or impedance around a nerve cell has also been reported. According to an exemplary embodiment of the present disclosure, the module controller 120 may measure impedance through a sine fitting method. Specifically, the module controller 120 may measure the impedance based on Equation 1.

[Equation 1]

Here, s(t) is a stimulus signal, r(t) is a received signal, z is a complex gain, g is an amplifier gain, j is an imaginary unit, and ω represents each frequency.

The complex gain may be calculated based on Equation 2.

[Equation 2]

Here, Re(.) is the real part of the complex gain z, Im(.) is the imaginary part of the complex gain, and T=2π/ω.

The module controller 120 may calculate a complex gain by applying a sine fitting method to both sine functions s(t) and r(t). Specifically, the module controller 120 may calculate an amplitude and a phase that minimizes Equation 3 by using a nonlinear least squares method.

[Equation 3]

Here, e is the Pring error, A is the estimated amplitude, and φ is the estimated phase.

The amplitude and phase of the complex gain may be calculated by taking the difference between the ratio of the amplitude and the phase calculated through Equation 3 as shown in Equation 4 below.

[Equation 4]

,

,

,

는 각각 s(t)와 r(t) 추정 진폭과 위상을 나타낸다.here

,

,

,

Represents the estimated amplitude and phase of s(t) and r(t), respectively.

Even when the frequency of the signal is known, the fitting method requires much more computation than the lock detection method. However, the calculation period of the ECIS-based cell culture chamber control device 100 of the present application is a few minutes, and the IoT-based device requires basic functions such as impedance estimation, so the limitation on the calculation time is relatively small. . Therefore, if there is a difference in noise, it would be advantageous to choose a better method among lock detection methods for the fitting method, regardless of what is required for the calculation.

The ECIS-based cell culture chamber control apparatus 100 may measure and record impedance according to a user request. In addition, the recorded impedance value or the trend of the impedance change may be provided to the user.

The database unit 130 may record impedance data of the cell culture chamber measured from the measurement module. In addition, the control signal may be transmitted to the module control unit 120 through the database unit 130. In other words, the database unit 130 may serve as a cloud. As the ECIS-based cell culture chamber control apparatus 100 is implemented in the form of an IOT, the user terminal and the module control unit 120 are not directly connected, and control signals may be exchanged through the database unit 130. This is to improve the degree of integration of the ECIS-based cell culture chamber control device 100 and to allow the module control unit 120 to focus on impedance measurement. The module controller 120 continuously monitors whether or not a control signal is generated, and when a control signal is received from a user terminal or a change in the control signal occurs, the module controller 120 may measure the impedance based on the recently received control signal. For example, the control signal may include at least one of a frequency, a channel, a measurement interval, an experiment period, and an experiment name.

In addition to the control signal, the communication unit 110 may receive various signals from the user terminal. For example, the communication unit 110 may receive a start signal and a stop signal. When the communication unit 110 does not receive a start signal or a stop signal, the module control unit 120 may continuously measure the impedance. For example, when the start signal is received, the module controller 120 may measure the impedance for each measurement interval included in the control signal received together with the start signal, and the database unit 130 may store the measured impedance. have. The database unit 130 may store the measured impedance in association with an identifier of the cell culture chamber and a measurement time.

According to the ECIS-based cell culture chamber control apparatus 100 of the present application, it is possible to reduce manufacturing cost and improve integration through the database unit 130 that includes an AFE and performs a cloud function. In other words, it is possible to save complex laboratory space by omitting the input and output devices, and the experiment can be controlled or monitored through a smart device without being located in front of the experiment equipment. In addition, since the database unit 130 can be connected to a network, information can be easily shared with experimental members.

6 is a view showing the flow of the ECIS-based cell culture chamber control method according to an embodiment of the present application.

The ECIS-based cell culture chamber control method shown in FIG. 6 may be performed by the ECIS-based cell culture chamber control apparatus described with reference to FIGS. 1 to 5 above. Therefore, even if omitted below, the description of the ECIS-based cell culture chamber control apparatus according to FIGS. 1 to 5 may be equally applied to FIG. 6.

Referring to FIG. 6, in step S610, the communication unit 110 may receive a control signal for controlling an electrode module and a measurement module from a user terminal. For example, the control signal may include at least one of a frequency, a channel, a measurement interval, an experiment period, and an experiment name.

In step S620, the module controller 120 may control the electrode module for applying electrical stimulation to each of the plurality of cell culture chambers and the measurement module for measuring impedance according to the electrical stimulation for each of the plurality of cell culture chambers. Specifically, the module controller 120 may change the electrical stimulation of the electrode module based on the control signal, and measure a change in impedance of the electrode due to cell growth according to the electrical stimulation through the measurement module. In addition, the module control unit 120 may include an analog front end (AFE) and a single board computer (SBC). In this case, the communication unit 110 may be connected to the SBC, and the electrode module and the measurement module may be connected to the AFE.

The measurement module may include a USB oscilloscope. Referring to FIG. 2, the USB oscilloscope may be connected to an AFE, and the AFE may be connected to an SBC through a USB interface. In addition, as the communication unit 110 is connected to the SBC, the SBC may be connected to the network through the communication unit 110.

The module controller 120 may generate a sine wave based on the function generator of the USB oscilloscope. For example, the sine wave may be a 1 Vpp sine file. In addition, the electrode module may apply electrical stimulation to a plurality of cell culture chambers through resistance of a preset size. In addition, the measurement module may non-invasively measure impedance due to currents flowing through a plurality of cell culture chambers according to a resistance of a preset size. The resistance of the preset size may be 1 MΩ by way of example. When an electrical stimulation is applied to any one of the plurality of cell culture chambers by the electrode module, since the current flowing through the chamber is less than 1 μA, non-invasive detection of the measurement module may be possible.

In addition, the module controller 120 may determine the amplifier type, the number of samples per period, and parameters of the impedance calculation method. According to an exemplary embodiment of the present disclosure, the module controller 120 may measure impedance through a sine fitting method. Specifically, the module controller 120 may measure the impedance based on Equation (5).

[Equation 5]

Here, s(t) is a stimulus signal, r(t) is a received signal, z is a complex gain, g is an amplifier gain, j is an imaginary unit, and ω represents each frequency.

The complex gain may be calculated based on Equation 6.

[Equation 6]

Here, Re(.) is the real part of the complex gain z, Im(.) is the imaginary part of the complex gain, and T=2π/ω.

The module controller 120 may calculate a complex gain by applying a sine fitting method to both sine functions s(t) and r(t). Specifically, the module controller 120 may calculate an amplitude and a phase that minimizes Equation 7 using a nonlinear least squares method.

[Equation 7]

Here, e is the Pring error, A is the estimated amplitude, and φ is the estimated phase.

The amplitude and phase of the complex gain may be calculated by taking the difference between the ratio of the amplitude and the phase calculated through Equation 7 as shown in Equation 8 below.

[Equation 8]

,

,

,

는 각각 s(t)와 r(t) 추정 진폭과 위상을 나타낸다.here

,

,

,

Represents the estimated amplitude and phase of s(t) and r(t), respectively.

In step S630, the database unit 130 may record impedance data of the cell culture chamber measured by the measurement module. In addition, the control signal may be transmitted to the module control unit 120 through the database unit 130. In other words, the database unit 130 may serve as a cloud. As the ECIS-based cell culture chamber control apparatus 100 is implemented in the form of an IOT, the user terminal and the module control unit 120 are not directly connected, and control signals may be exchanged through the database unit 130. In addition, the database unit 130 may store the measured impedance in association with the identifier of the cell culture chamber and the measurement time.

The ECIS-based cell culture chamber control method according to an embodiment of the present application may be implemented in the form of a program command that can be executed through various computer means and recorded in a computer-readable medium. The computer-readable medium may include program instructions, data files, data structures, etc. alone or in combination. The program instructions recorded in the medium may be specially designed and configured for the present invention, or may be known and usable to those skilled in computer software. Examples of computer-readable recording media include magnetic media such as hard disks, floppy disks, and magnetic tapes, optical media such as CD-ROMs and DVDs, and magnetic media such as floptical disks. -A hardware device specially configured to store and execute program instructions such as magneto-optical media, and ROM, RAM, flash memory, and the like. Examples of the program instructions include not only machine language codes such as those produced by a compiler, but also high-level language codes that can be executed by a computer using an interpreter or the like. The above-described hardware device may be configured to operate as one or more software modules to perform the operation of the present invention, and vice versa.

The foregoing description of the present application is for illustrative purposes only, and those of ordinary skill in the art to which the present application pertains will be able to understand that it is possible to easily transform it into other specific forms without changing the technical spirit or essential features of the present application. Therefore, it should be understood that the embodiments described above are illustrative in all respects and not limiting. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as being distributed may also be implemented in a combined form.

The scope of the present application is indicated by the claims to be described later rather than the detailed description, and all changes or modified forms derived from the meaning and scope of the claims and their equivalent concepts should be interpreted as being included in the scope of the present application.

100: ECIS-based cell culture chamber control device
110: communication department
120: module control unit
130: database unit

Claims (16)

In the ECIS-based cell culture chamber control device,
A communication unit receiving a control signal for controlling a measurement module including an electrode module and a USB oscilloscope from a user terminal;
A module controller for controlling the electrode module for applying electrical stimulation to each of the plurality of cell culture chambers and the measurement module for measuring impedance for each of the plurality of cell culture chambers; And
Including a database unit for recording impedance data of the cell culture chamber measured from the measurement module,
The control signal is transmitted to the module control unit through the database unit,
The module control unit,
Changing the electrical stimulation of the electrode module based on the control signal, and measuring a change in impedance of the electrode due to cell growth according to the electrical stimulation through the measurement module,
The module control unit,
Including an analog front end (AFE) and a single board computer (SBC),
The communication unit is connected to the SBC, the electrode module and the measurement module are connected to the AFE, the electrode module, the measurement module and the USB oscilloscope are connected to the AFE,
The AFE is connected to the SBC through a USB interface,
The control signal is transmitted and received between the user terminal and the module control unit through the database unit, ECIS-based cell culture chamber control apparatus. delete The method of claim 1,
The module control unit,
Generate a sine wave based on the function generator of the USB oscilloscope,
The electrode module is to apply electrical stimulation to the plurality of cell culture chambers through a resistance of a preset size, ECIS-based cell culture chamber control device. The method of claim 3,
The measurement module,
The ECIS-based cell culture chamber control device to non-invasively measure the impedance of the current flowing through the plurality of cell culture chambers according to the resistance of the preset size. The method of claim 1,
The control signal is
The ECIS-based cell culture chamber control device comprising at least one of frequency, channel, measurement interval, and experimental period. The method of claim 1,
The module control unit,
To measure the impedance based on Equation 1, ECIS-based cell culture chamber control apparatus.
[Equation 1]

Here, s(t) is the stimulus signal, r(t) is the received signal, z is the complex gain, g is the amplifier gain, j is the imaginary unit, and ω is the angular frequency. The method of claim 6,
The complex gain is calculated based on Equation 2, ECIS-based cell culture chamber control apparatus.
[Equation 2]

Here, Re(.) is the real part of the complex gain z, Im(.) is the imaginary part of the complex gain, and T=2π/ω. The method of claim 6,
The module control unit,
To estimate the amplitude and phase of the stimulation signal to minimize Equation 3, ECIS-based cell culture chamber control device.
[Equation 3]

Here, e is the Pring error, A is the estimated amplitude, and φ is the estimated phase. In the ECIS-based cell culture chamber control method,
(a) receiving, by a communication unit, a control signal for controlling a measurement module including an electrode module and a USB oscilloscope from a user terminal;
(b) controlling, by a module control unit, the electrode module for applying electrical stimulation to each of the plurality of cell culture chambers and the measurement module for measuring impedance according to the electrical stimulation for each of the plurality of cell culture chambers; And
(c) comprising the step of recording the impedance data of the cell culture chamber measured by the database unit from the measurement module,
The step (b),
Changing the electrical stimulation of the electrode module based on the control signal, and measuring a change in impedance of the electrode due to cell growth according to the electrical stimulation through the measurement module,
The module control unit,
Including an analog front end (AFE) and a single board computer (SBC),
The communication unit is connected to the SBC, the electrode module and the measurement module are connected to the AFE, the electrode module, the measurement module, and the USB oscilloscope are connected to the AFE,
The AFE is connected to the SBC through a USB interface,
The control signal is transmitted and received between the user terminal and the module control unit through the database unit, ECIS-based cell culture chamber control method. The method of claim 9,
The step (b),
Generate a sine wave based on the function generator of the USB oscilloscope,
The electrode module is to apply electrical stimulation to the plurality of cell culture chambers through a resistance of a preset size, ECIS-based cell culture chamber control method. The method of claim 10,
The measurement module,
To non-invasively measuring the impedance of the current flowing through the plurality of cell culture chambers according to the resistance of the preset size, ECIS-based cell culture chamber control method. The method of claim 9,
The control signal is
The ECIS-based cell culture chamber control method comprising at least one of frequency, channel, measurement interval, and experimental period. The method of claim 9,
The step (b),
To measure the impedance based on Equation 4, ECIS-based cell culture chamber control method.
[Equation 4]

Here, s(t) is the stimulus signal, r(t) is the received signal, z is the complex gain, g is the amplifier gain, j is the imaginary unit, and ω is the angular frequency. The method of claim 13,
The complex gain is calculated based on Equation 5, ECIS-based cell culture chamber control method.
[Equation 5]

Here, Re(.) is the real part of the complex gain z, Im(.) is the imaginary part of the complex gain, and T=2π/ω. The method of claim 13,
The step (b),
To estimate the amplitude and phase of the stimulation signal to minimize Equation 6, ECIS-based cell culture chamber control method.
[Equation 6]

Here, e is the Pring error, A is the estimated amplitude, and φ is the estimated phase. A computer-readable recording medium storing a program for executing the method of claim 9 on a computer.

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