KR102194311B1 - Patch for superabsorbent resin comprising latic acid bacteria natural liposome and aloe natural liposome, prepatation thereof, and hygiene product - Google Patents

Abstract

The present invention relates to a patch for a super absorbent polymer comprising a lactic acid bacteria-containing natural liposome and aloe-containing natural liposome, a method for producing the same, and a hygiene product to which the patch is applied.

Description

[PATCH FOR SUPERABSORBENT RESIN COMPRISING LATIC ACID BACTERIA NATURAL LIPOSOME AND ALOE NATURAL LIPOSOME, PREPATATION THEREOF, AND HYGIENE PRODUCT}, a patch for superabsorbent resin containing natural liposomes and aloe natural liposomes, and a manufacturing method thereof.

The present invention relates to a patch for a superabsorbent polymer comprising a lactic acid bacteria natural liposome and aloe natural liposome, and to a hygiene product including the same, for example, a diaper, a sanitary napkin.

Sanitary pads used to absorb secretions such as menstrual blood and cold water cause discomfort due to the odor from the secretions and cause unpleasant irritation to the human body due to the secretions, such as itching and skin soreness. This problem is more serious in that the sanitary pad is worn and used for a long time. The reason for this problem is that the secretion maintains a humid state, thereby creating favorable conditions for the habitat of bacteria and fungi.

Accordingly, attempts to solve these problems by suppressing the occurrence of bacteria and fungi and increasing the proliferation of beneficial lactic acid bacteria are being continued.

For example, vaginal lactic acid bacteria formulations have been developed to improve vaginal health, but there are not many lactic acid bacteria reaching the vagina, and there is a problem that it takes a long time for the lactic acid bacteria to stably reproduce.

On the other hand, Korean Patent Publication No. 2017-0071919 discloses a diaper coated with lactic acid bacteria including one or more strains derived from the genus Lactobacillus.

However, there is still a need to sufficiently solve problems such as odor and irritation.

Korean Patent Publication No. 2017-0071919

In order to solve the above problems, an object of the present invention is to provide a patch for a super absorbent polymer, such as a diaper and sanitary napkin, which is harmless to the human body and less irritating or odorous, a manufacturing method thereof, and a hygiene product including the same.

An example of the present invention is (a) lactic acid bacteria as a core; (b) a first shell layer surrounding the core and comprising a natural polysaccharide; (c) a second shell layer surrounding the first shell layer and comprising a first natural emulsifier and a natural protein; And (d) surrounding the second shell layer, and containing a third shell layer containing a natural cryoprotectant, a natural liposome containing lactic acid bacteria, and a liposome containing at least one second natural emulsifier, a natural solvent, a natural coating agent, and a natural preservative. Constituent material; And aloe powder as a trapping material, the second natural emulsifier, the naturally derived solvent, and the aloe-containing natural liposome formed by ultrasonically mixing the aloe powder.

Another example of the present invention is (i) a first step of ultrasonic treatment by adding a second natural emulsifier to a solvent of 40 to 75°C; (ii) a second step of adding and mixing lactic acid bacteria after ultrasonic treatment by adding natural proteins and natural polysaccharides to a solvent at 40 to 75°C; (iii) a third step of mixing the product obtained in the first step, the product obtained in the second step, and a natural cryoprotectant; And (iv) a fourth step of lyophilizing the product obtained in the third step, and a method of manufacturing the patch for a super absorbent polymer is provided.

Another example of the present invention provides a hygiene product including the super absorbent polymer patch.

The patch for a super absorbent polymer according to the present invention has excellent antibacterial and deodorizing effects, so when used as hygiene products such as diapers and sanitary napkins, it is possible to reduce discomfort of users, increase convenience, and improve health status.

1 is a photograph showing the result of confirming the growth of lactic acid bacteria according to Experimental Example 1.
2 is a photograph showing the antibacterial effect against E. coli according to Experimental Example 2.
3 is a photograph showing the effect of inhibiting E. coli growth according to Experimental Example 3.

Hereinafter, a patch for a superabsorbent polymer containing a lactic acid bacteria natural liposome and aloe natural liposome according to the present invention will be described in more detail with reference to the accompanying drawings. However, these descriptions are merely presented as an example to aid understanding of the present invention, and the scope of the present invention is not limited by these exemplary descriptions.

<Lactobacillus natural liposome>

Lactobacillus natural liposomes used in the super absorbent polymer patch of the present invention include (a) lactic acid bacteria as a core; (b) a first shell layer surrounding the core and comprising a natural polysaccharide; (c) a second shell layer surrounding the first shell layer and comprising a first natural emulsifier and a natural protein; And (d) a third shell layer surrounding the second shell layer and containing a natural cryoprotectant. In this case, the third shell layer may further include a natural preservative.

The lactic acid bacteria natural liposomes in the present invention made of natural substances are harmless because they are not toxic to the human body, and the lactic acid bacteria can stably survive by forming a stable phospholipid bilayer.

Since these lactic acid bacteria natural liposomes are in the form of surrounding lactic acid bacteria in multiple layers, it can be prevented that the lactic acid bacteria are destroyed or the activity of the lactic acid bacteria decreases.

Hereinafter, the lactic acid bacteria natural liposomes will be described by dividing each layer as follows.

(a) Core: lactic acid bacteria

Lactobacillus natural liposome with a three-layer structure is located in the core (Core).

The lactic acid bacteria may be a strain corresponding to a fungus such as commonly known Lactobacillus, Lactococcus, Leuconostoc, Pediococcus, and Bifidobacterium. . Specifically, Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus gasseri , Lactobacillus helveticus, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus rhamnosus, Lactobacillus reactobacillus terri Salivarius (Lactobacillus salivarius), Lactococcus lactis, Streptococcus thermophilus, Bifidobacterium animalis ssp. lactis, Bifidobacterium Bifidobacterium bifidum), Bifidobacterium breve, Bifidobacterium longum, and Enterococcus faecalis.It may be one or more selected from the group consisting of.

According to an example of the present invention, the lactic acid bacteria may be one selected from the group consisting of Lactobacillus acidophilus, Bifidobacterium bifidum, and Bifidobacterium longum.

According to another embodiment of the present invention, the lactic acid bacteria are Lactobacillus acidophilus, Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus rhamnosus, Streptococcus thermophilus, Bifidobacterium animalis lactis, and It may be one or more selected from the group consisting of Bifidobacterium long gum.

When forming a core using these lactic acid bacteria, it is preferable to use a homogenizer and/or a stirrer. This is because the ultrasonic crusher used to form the first shell layer and the second shell layer generates heat and vibration, and thus lactic acid bacteria may be destroyed or damaged.

(b) first shell layer: natural polysaccharide

The first shell layer is a layer surrounding the lactic acid bacteria and contains a natural polysaccharide to protect the lactic acid bacteria.

The natural polysaccharides are those extracted and purified from natural substances, such as Arabic gum, Guar gum, Locust bean gum, Tamarind gum, and Tara gum. , Xanthan gum (Xanthan gum) and carrageenan (Carrageenan) can be used one or more selected from the group consisting of. Preferably, at least one selected from the group consisting of locust bean gum, tamarind gum, and tara gum may be used.

When forming the first shell layer using such a natural polysaccharide, an ultrasonic crusher may be used so that the natural polysaccharide can be uniformly dispersed in a solvent.

(c) second shell layer: first natural emulsifier and natural protein

The second shell layer is a layer surrounding the natural polysaccharide and includes a first natural emulsifier and a natural protein.

The first natural emulsifier is an emulsifier derived from a natural substance and is a material constituting the lipid bilayer. The first natural emulsifier is a phospholipid composed of a water-soluble phosphoric acid and a fat-soluble fatty acid, and forms a lipid bilayer. The phospholipid contained in the first natural emulsifier is a phospholipid derived from nature, and a fatty acid is also a natural fatty acid derived from nature.

The first natural emulsifier is selected from the group consisting of phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanolamine, phosphatidylic acid, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, and hydrogenated products thereof (eg, soy-derived hydrogenated phosphatidylcholine). One or more of these can be used. At this time, it is preferable to use phosphatidylcholine, lysophosphatidylcholine, or a hydrogenated product thereof (hydrogenated phosphatidylcholine) having excellent oxidation safety. The fatty acids are largely contained in nuts such as walnuts, pecans, almonds, pine nuts, and seeds such as olives, corn, flax, cotton, rape flowers, and evening primroses, and can be obtained by extraction and separation and purification from these. As the fatty acid, at least one natural fatty acid selected from the group consisting of palmitic acid, stearic acid, oleic acid, linoleic acid, and linolenic acid may be used. have.

As the natural protein, both extracted and purified from natural substances may be used. The natural protein can be used as a nutrient source for growth and proliferation of lactic acid bacteria. At this time, it is preferable to use milk-derived protein and/or soy-derived protein suitable as a nutrient source for lactic acid bacteria, and particularly, it is more preferable to use whey protein.

When forming the second shell layer using the first natural emulsifier and natural protein, an ultrasonic grinder may be used so that the first natural emulsifier and natural protein can be uniformly dispersed in a solvent.

(d) third shell layer: natural cryoprotectant

The third shell layer is an outermost layer surrounding the second shell layer containing the first natural emulsifier and natural protein, and contains a natural cryoprotectant to prevent the lactic acid bacteria or the multilayer structure surrounding the lactic acid bacteria from being destroyed during freeze drying.

As the natural cryoprotectant, at least one selected from the group consisting of skim milk powder, maltodextrin, and glycerin may be used. In this case, in order to increase the cryoprotective effect, it is preferable to use two or more types in combination, and skim milk powder and glycerin or maltodextrin and glycerin may be used.

This third shell layer may further include a natural preservative together with the natural cryoprotectant. The natural preservative is a substance that maintains the stability of the final natural liposome by preventing the prepared lactic acid bacteria-containing liposome from being spoiled during storage and distribution.

As the natural preservative, as a natural preservative that can be added to food, at least one selected from the group consisting of citrus extract, gosam extract, citron extract, eucalyptus extract, grapefruit extract, clove extract, and cranberry extract may be used. At this time, it is preferable to use a citrus extract, a grapefruit extract, and a cranberry extract in order to increase the preservation power, and it is more preferable to use a grapefruit extract and a cranberry extract to show more improved preservation power. The cranberry extract can improve the survival rate of lactic acid bacteria while controlling the growth of harmful bacteria, thereby further improving the physiological activity effect of the lactic acid bacteria. Since the preservative is a natural preservative, dehydroacetic acid, sorbic acid, benzoic acid, methyl ρ-hydroxybenzoate, and propanoic acid are commonly used as preservatives. And it is distinguished from chemical preservatives such as salts thereof, and is harmless to the human body because it is not toxic.

Such lactic acid bacteria natural liposomes are in the form of capsules surrounding the lactic acid bacteria in three layers. The lactic acid bacteria natural liposome is based on the total weight, based on the total weight, lactic acid bacteria 1 to 20% by weight, natural polysaccharide 0.5 to 5% by weight, the first natural emulsifier 5 to 30% by weight, natural protein 0.5 to 10% by weight and natural cryoprotectant 45 to 75 Including% by weight or based on the total weight, 1 to 20% by weight of lactic acid bacteria, 0.5 to 5% by weight of natural polysaccharide, 5 to 30% by weight of the first natural emulsifier, 0.5 to 10% by weight of natural protein, natural cryoprotectant 40 To 70% by weight and 5 to 15% by weight of a natural preservative.

These lactic acid bacteria natural liposomes are surrounded by a first shell layer, a second shell layer, and some third shell layers, and are protected from the outside.

<Method of manufacturing natural liposomes of lactic acid bacteria>

These lactic acid bacteria natural liposomes are prepared using an ultrasonic disruptor (Ultrasonicator), a homogenizer (Homogenizer). However, it is not limited only by this method, and the steps of each process may be modified or selectively mixed and performed as necessary.

The manufacturing method of natural lactic acid bacteria liposome includes (i) a first step of ultrasonic treatment by adding a first natural emulsifier to a solvent of 40 to 75°C; (ii) a second step of adding and mixing natural proteins and natural polysaccharides in a solvent of 40 to 75°C, and then adding and stirring lactic acid bacteria; (iii) a third step of mixing the product obtained in the first step, the product obtained in the second step, and a natural cryoprotectant; And (iv) a fourth step of adding a natural preservative to the product obtained in the third step.

Hereinafter, the manufacturing method will be described by dividing each process step as follows.

First Step: Ultrasonic treatment is performed by adding a first natural emulsifier to a solvent at 40 to 75°C.

As the solvent, one or more selected from the group consisting of a naturally derived solvent such as distilled water, naturally derived butylene glycol, natural propanediol, natural glycerin, natural ethanol, and fermented alcohol may be used. Preferably distilled water, naturally derived glycerin, or natural ethanol may be used, and more preferably distilled water may be used. The naturally-derived solvent allows the lipid bilayer of the first natural emulsifier to form a capsule surrounding the trapping material.

The first natural emulsifier is an emulsifier derived from a natural substance and is a material constituting the lipid bilayer, and the first natural emulsifier that can be used is as described above.

The ultrasonic treatment is to sufficiently disperse the first natural emulsifier in a solvent, and a general ultrasonic crusher may be used. In order to disperse more efficiently, it is preferable to use a continuous circulating ultrasonic disperser (Ultrasonic Liquid processor).

The first natural emulsifier may be uniformly dispersed in the solvent by heating such a solvent to 40 to 75° C. and ultrasonic treatment by adding the first natural emulsifier. At this time, a stirrer may also be used, and may be stirred at 1,500 to 5,500 rpm for 3 to 30 minutes.

Step 2: After mixing by adding natural protein and natural polysaccharide to a solvent of 40 to 75°C, lactic acid bacteria are added and stirred.

As the solvent, the same solvent as the solvent used in the first step may be used, or another solvent may be used.

The natural protein is extracted and purified from a natural substance, and may be a milk-derived protein (eg, whey protein) and/or a soy-derived protein.

The natural polysaccharide is extracted and purified from natural substances, such as Arabic gum, Guar gum, Locust bean gum, Tamarind gum, Tara gum, At least one selected from the group consisting of Xanthan gum and Carrageenan may be used.

The lactic acid bacteria include Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus gasseri, and Lactobacillus gasseri. , Lactobacillus helveticus, Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus rhamnosus, Lactobacillus reactobacillus terri Salivarius (Lactobacillus salivarius), Lactococcus lactis (Lactococcus lactis), Streptococcus thermophilus (Streptococcus thermophilus), Bifidobacterium animalis ssp. lactis (Bifidobacterium animalis ssp. lactis), Bifidobacterium bifidum ( Bifidobacterium bifidum), Bifidobacterium breve, Bifidobacterium longum (Bifidobacterium longum) and Enterococcus pecalis (Enterococcus faecalis) It may be one or more selected from the group consisting of.

After heating such a solvent to 40 to 75°C, natural protein and natural polysaccharide are added and mixed, so that the natural protein and natural polysaccharide in the solvent can be uniformly dispersed.

Then, lactic acid bacteria are added and stirred. Since lactic acid bacteria are weak to heat, it is preferable to add lactic acid bacteria when the temperature of the solvent does not affect the activity of the lactic acid bacteria, that is, when the temperature of the solvent is less than 50°C as described above. At this time, it can be stirred for 3 to 30 minutes at 1,500 to 5,500 rpm.

Step 3: The product of the first step, the product of the second step, and the natural cryoprotectant are mixed.

The natural cryoprotectant may prevent the lactic acid bacteria or the multi-layer structure surrounding the lactic acid bacteria from being destroyed when lyophilizing the lactic acid bacteria surrounded by multi-layers. As the natural cryoprotectant, at least one selected from the group consisting of skim milk powder, maltodextrin, and glycerin may be used, and two or more may be mixed to increase the effect of the cryoprotectant.

After sufficiently mixing the product obtained in the first step and the product obtained in the second step, a natural cryoprotectant is added and mixed. At this time, a homogenizer and a stirrer may be used, and the mixture may be stirred at 1,500 to 5,500 rpm for 3 to 30 minutes.

Step 4: Add a natural preservative to the product of Step 3.

A natural preservative is further added to the product obtained in the third step. As the natural preservative, at least one selected from the group consisting of citrus extract, gosam extract, citron extract, eucalyptus extract, grapefruit extract, cloves extract, and cranberry extract may be used.

The final product is prepared by adding and mixing a natural preservative to the product obtained in the third step. Shake enough to mix.

According to another example of the present invention, the lactic acid bacteria natural liposome is (i) a first step of mixing by adding a natural polysaccharide to a solvent at 40 to 75°C, and then adding and stirring the lactic acid bacteria; (ii) a second step of ultrasonic treatment by adding a first natural emulsifier to a solvent at 40 to 75°C; (iii) a third step of mixing by adding natural protein to a solvent of 40 to 75°C; (iv) The product obtained in the first step, the product obtained in the second step, the product obtained in the third step, and a natural cryoprotectant may be mixed in the fourth step. In this case, a natural preservative may be further added and mixed in the fifth step.

In the method for preparing a natural liposome containing lactic acid bacteria, the material used in each step is the same as the method for preparing a natural liposome containing lactic acid bacteria according to the above example.

Through such a manufacturing method, natural liposome particles containing lactic acid bacteria having a three-layer structure can be formed. These natural liposomes containing lactic acid bacteria are powdered to facilitate storage and packaging, and the proliferation of spoilage bacteria is inhibited, so that they can be stored for a long time even at low and room temperature.

The natural liposome containing lactic acid bacteria of the present invention has excellent acid resistance and is safely present with a high survival rate, and can exhibit various physiological effects from such lactic acid bacteria.

The natural liposome containing lactic acid bacteria is a component derived from a natural substance, and does not exhibit side effects even when it comes into contact with the human body. When using hygiene products containing these lactic acid bacteria natural liposomes, various diseases can be prevented.

<Aloe natural liposome>

Aloe natural liposome according to an embodiment of the present invention comprises at least one second natural emulsifier, a naturally derived solvent, a natural coating agent, a liposome constituent material containing a natural preservative; And aloe powder as a collecting material, and is formed by mixing the second natural emulsifier, the naturally derived solvent, and the aloe powder by ultrasonic treatment. Take a look at each of these ingredients.

1. Liposomal components

The liposome of the present invention is a natural liposome comprising a second natural emulsifier, a naturally derived solvent, a natural coating agent and a natural preservative. Natural liposomes made of these natural substances are harmless because they are not toxic to the human body, and can be ingested. In addition, the liposome forms a stable phospholipid bilayer, so that the trapping material can be effectively delivered to cells.

[Second natural emulsifier]

The second natural emulsifier is a substance constituting the lipid bilayer, and is an emulsifier derived from natural substances. The second natural emulsifier that can be used is as mentioned in the lactic acid bacteria natural liposome.

[menstruum]

As the solvent, a naturally derived solvent is used. The solvent that can be used is as mentioned in the lactic acid bacteria natural liposome. The content of the solvent is preferably the remaining amount excluding the second natural emulsifier, natural coating agent, natural preservative, and trapping material based on the weight of the total aloe natural liposome.

[antiseptic]

As the preservative, any natural preservative can be used without limitation. For example, natural extracts can be used. One or more selected from the group consisting of citrus extract, citron extract, eucalyptus extract, gosam extract, cloves extract, grapefruit extract, chopidae fruit extract, pasqueflower (baekduong) extract, and Earthier extract may be used. Preferably grapefruit extract, citrus extract. Berries extract can be used. In that it is a natural extract, it is distinguished from commonly used benzoic acids, sorbic acids, propionic acids, dehydroacetic acids, and paraoxybenzoic acids.

The content of the preservative is preferably 0.001 to 3% by weight, and more preferably 0.01 to 0.3% by weight, based on the total weight of the natural aloe liposome.

2. Collected material

The trapping material according to the present invention is a material trapped inside the lipid bilayer of the liposome containing the second natural emulsifier, a naturally derived solvent, a natural coating agent, and a natural preservative. Aloe powder is used as the trapping material. Aloe powder works to improve gut health and boost immunity.

The content of the trapping material is preferably 0.1 to 30% by weight based on the total weight of the natural liposome.

3. Other

A coating agent may be additionally included as a component for coating other trapping materials. As the coating agent, a natural coating agent is preferably used. Dietary fiber, starch, polysaccharide, etc. may be used as the coating agent. In addition, prebiotics, which can be food for lactic acid bacteria, may be additionally included. As a prebiotic, fructo-oligosaccharides not only serve as food for lactic acid bacteria, but also act as a type of polysaccharide as a coating agent.

For example, maltodextrin, tapioca, guar gum, corn starch, guarana extract, and the like may be used. It is preferable to use maltodextrin and guarana extract powder from the viewpoint of high stability so that the trapped material is well absorbed.

In addition, an absorption aid may be additionally included in order to help the liposome surrounding the collecting material to be well absorbed in the body. As an absorption aid, a plant extract powder containing a large amount of caffeine may be used, and for example, a guarana extract powder may be used. In addition, Indian gooseberry extract can also be used. Aloe natural liposomes containing a natural absorption aid can exhibit excellent effects by aloe because the absorption rate of the trapping material is increased in the body.

The content of the natural absorption aid of the natural coating agent is preferably 0.001 to 3% by weight, respectively, based on the total weight of the natural aloe liposome.

As described above, the natural aloe liposome of the present invention is in the form of a capsule surrounding aloe with a lipid bilayer, and can protect aloe well.

<Method of manufacturing natural liposomes containing aloe>

Aloe-containing natural liposomes according to an embodiment of the present invention include: (a) a first step of heating a naturally-derived solvent to 35°C to 80°C, adding at least one second natural emulsifier, mixing, and ultrasonic treatment; (b) a second step of adding guarana powder and fructo-oligosaccharide to the mixture of the first step, followed by ultrasonic treatment at 35°C to 80°C to mix; (c) a third step of adding aloe powder to the mixture of the second step and ultrasonicating at 35°C to 80°C; And (d) adding a naturally derived solvent and a natural preservative to the mixture of the third step, and performing ultrasonic treatment at 35°C to 80°C. After the fourth step, a fifth step of repeatedly performing ultrasonic treatment while gradually lowering the temperature may be further included.

Hereinafter, the manufacturing method will be described by dividing each process step as follows.

(a) Step 1

This step is a step of heating a naturally derived solvent to 35°C to 80°C, adding at least one second natural emulsifier, mixing, and ultrasonic treatment.

After the naturally derived solvent is heated to 35°C to 80°C, preferably 50°C to 75°C, more preferably 60°C to 70°C, at least one second natural emulsifier is added thereto. The temperature range is an advantageous temperature range for the emulsifier to stably form liposomes.

After adding a natural emulsifier, it is dissolved by stirring at 2,500 to 4.500 rpm for 3 to 30 minutes. Subsequently, by ultrasonic crushing, the phospholipids and fatty acids of the second natural emulsifier can be homogenized in a naturally derived solvent.

The ultrasonic treatment is an operation for homogenizing a mixture in which a naturally derived solvent and a second natural emulsifier are mixed, and an ultrasonic crusher (Ultrasonic Liquid processor) may be used.

(b) the second step

This step is a step of adding guarana powder and fructo-oligosaccharide to the mixture of the first step, stirring, and sonicating at 35°C to 80°C to mix.

Stirring may be performed at 1,500 to 5,500 rpm for 3 to 30 minutes.

The ultrasonic treatment may be performed after, for example, adding guarana powder and fructo-oligosaccharide to the mixture in the first step, stirring, heating to 35°C to 80°C, and mixing. However, when the mixture in the first step maintains the temperature range, ultrasonic treatment may be performed without a separate heating process.

Through the second step, each component can be homogeneously mixed and a liposome having a small particle size can be formed.

In the second step, Guarana powder plays a role in helping the natural liposomes finally obtained to be well absorbed.

On the one hand, fructo-oligosaccharides act as prebiotics and are safely used as food for lactic acid bacteria to suppress harmful bacteria.On the other hand, it acts as a natural coating agent, so that natural liposomes are safely absorbed into the body, and the health of the skin barrier. It can also help you keep it.

(c) Step 3

This step is a step of adding aloe powder to the mixture of the second step, stirring, and ultrasonicating at 35°C to 80°C.

Stirring may be performed at 1,500 to 5,500 rpm for 3 to 30 minutes.

The ultrasonic treatment may be performed, for example, after adding aloe powder to the mixture in the second step, stirring, heating to 35°C to 80°C, and mixing. However, when the mixture in the second step maintains the temperature range, ultrasonic treatment may be performed without a separate heating process.

(d) Step 4

In this step, a naturally derived solvent and a natural preservative are added to the mixture of the third step, stirred, and subjected to ultrasonic treatment at 35°C to 80°C. A naturally derived solvent or a naturally derived solvent and a natural preservative may be preheated to the above temperature, followed by ultrasonication.

As the naturally derived solvent, the same solvent as the solvent used in the first step may be used.

The natural preservative maintains the stability of natural liposomes by preventing spoilage of liposome constituents (second natural emulsifier, natural solvent, natural coating agent) and trapping material present in the liposome. In addition, the natural preservatives are derived from natural substances and are not toxic and are harmless to the human body and do not damage natural liposomes.

Stirring may be performed at 1,500 to 5,500 rpm for 3 to 30 minutes.

In (b) the second step, (c) the third step, and (d) the fourth step, the ultrasonic treatment is performed at 35°C to 80°C, preferably at 60°C to 70°C.

(e) Step 5

In addition, in addition to steps (a) to (d), a step of repeatedly ultrasonicating while gradually lowering the temperature after step 4 may be performed. By gradually lowering the temperature of the prepared liposome, preferably at 35°C to 70°C, and finally by ultrasonication for 1 to 10 minutes, preferably 3 to 8 minutes, a smaller and more uniform final containing natural liposome is prepared. . The ultrasonic treatment may be repeatedly performed to prepare natural liposomes having a desired particle size, and preferably 1 to 3 times.

The natural liposome according to the present invention is prepared by a method of ultrasonic treatment so that the trapping material and the liposome constituent material are uniformly mixed. However, it is not limited only by this manufacturing method, and the steps of each process may be modified or selectively mixed and performed as necessary.

3. Other

A coating agent may be additionally included as a component for coating other trapping materials. As the coating agent, a natural coating agent is preferably used. Dietary fiber, starch, polysaccharide, etc. may be used as the coating agent, and for example, maltodextrin, tapioca, guar gum, corn starch, and the like may be used. It is preferable to use maltodextrin in terms of high stability so that the trapping material is well absorbed.

<Patch for super absorbent polymer>

The lactic acid bacteria natural liposome solution and the aloe natural liposome solution are applied to the fabric, respectively, or the liposome solution is mixed and applied to the fabric and dried to prepare a patch. The patch may include the lactic acid bacteria-containing natural liposome and the aloe-containing natural liposome in a weight ratio of 1:0.5 to 1:2. The patch can be used for super absorbent polymers such as sanitary napkins and diapers.

The present invention relates to a functional patch in which a liposome powder encapsulating lactic acid bacteria beneficial to vaginal health is applied to a pad. In more detail, it relates to a patch for a super absorbent polymer made by mixing lactic acid bacteria liposomes and aloe liposomes that help proliferate lactic acid bacteria and then applying them to the upper surface of fabrics including non-woven fabrics. By embedding the patch according to the present invention into a functional sanitary napkin, antibacterial and deodorizing effects are implemented, and the health condition of a person using a sanitary pad can be improved.

Hereinafter, the present invention will be described in detail through examples, but the following examples are only illustrative of one aspect of the present invention, and the scope of the present invention is not limited by the following examples.

[ Example 1] Natural lactic acid bacteria Liposome Aloe natural Liposome Containing patch

[ Example 1-1] Natural lactic acid bacteria Liposomal Produce

According to the composition of Table 1 below, lactic acid bacteria natural liposomes were prepared.

First, distilled water was heated to 52° C. in a reactor, and then DS-Soya PC 60IP was added and dispersed at 5,000 rpm for 5 minutes, followed by rotation and ultrasonic treatment to prepare a first mixture.

After heating distilled water to 45° C. in another reactor, a mixture of whey protein and polysaccharide was slowly added, mixed at 3,000 rpm for 5 minutes, and sonicated. Then, the lactic acid bacteria mixture was added and mixed at 5,000 rpm for 20 minutes to prepare a second mixture.

After the second mixture was added to the reactor in which the first mixture was prepared, maltodextrin and glycerin were added, followed by stirring at 5,000 rpm for 20 minutes. Then, grapefruit seed extract and cranberry concentrate were added and sufficiently mixed to prepare a natural liposome solution for lactic acid bacteria.

ingredient weight% 1st mixture Distilled water 48.7 DS-Soya PC60IP 3.0 2nd mixture Distilled water 31.68 Lactobacillus mixture (Lactobacillus acidophilus, Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus rhamnosus, Streptococcus thermophilus, Bifidobacterium animalis lactis and Bifidobacterium long gum) 2.0 Whey protein 1.0 Polysaccharide mixture (Biogel Mix (K-1)) 0.3 Maltodextrin 10.0 glycerin 1.0 Grapefruit seed extract 0.12 Cranberry concentrate 2.20

[ Example 1-2] Aloe natural Liposomal Produce

According to the composition of Table 2 below, aloe-containing natural liposomes were prepared.

ingredient weight% One Distilled water 35.0 DS-Soya PC60IP 3.0 2 Guarana Extract Powder 0.5 Fructo-oligosaccharide 5.0 Aloe Vera Gel Powder 5.0 3 Grapefruit seed extract 0.05 4 Distilled water 51.45

As shown in Table 2, first, distilled water was heated to 70° C. in the reactor, and then DS-Soya PC60 IP (100% soy lecithin containing 60.5% phosphatidylcholine and 1.9% lysophosphatidylcholine) was added as a second natural emulsifier and 3,000 It was dissolved by stirring at rpm for 5 minutes. After that, ultrasonic crushing was performed using an ultrasonic crushing machine (Ultrasonic Liquid Processor, QSONICA, USA).

Then, when the second natural emulsifier was completely dissolved by ultrasonic crushing and homogenized, guaranha extract powder and fructo-oligosaccharide were added thereto, stirred at 3000 rpm for 20 minutes, and then crushed through ultrasonic treatment.

When completely homogenized, aloe vera gel powder was added thereto, stirred at 3000 rpm for 20 minutes, and then ultrasonically crushed.

Thereafter, grapefruit seed extract and distilled water were added, heated to 60° C., stirred at 3000 rpm for 30 minutes, and then ultrasonically crushed to prepare a natural aloe liposome solution.

[ Example 1-3] Lactobacillus natural Liposome And aloe natural Liposome Containing patch

The lactic acid bacteria natural liposome solution of Example 1-1 and the aloe natural liposome solution of Example 1-2 were mixed at a weight ratio of 1:2, applied to the patch, and dried. The applied amount per patch was 120 mg.

[ Example 2] Lactobacillus composition

According to the composition of Table 3 below, a lactic acid bacteria composition containing the lactic acid bacteria natural liposomes of Example 1-1 as an active ingredient was prepared, applied to a patch, and dried.

ingredient weight% Lactobacillus natural liposome of Example 1-1 10 Aloe vera gel cellposome of Example 1-2 12 Complex lactic acid bacteria 5 Fructooligosaccharide 10 Galactooligosaccharide 2 Inulin 24 Indigestible maltodextrin 8 Xylitol 8 Acacia dietary fiber 21

[ Comparative example 1~3]

A general pad (product name: white) that does not contain lactic acid bacteria and aloe at all was used as Comparative Example 1, and an herbal patch (product name: Gwiaerang) that does not contain lactic acid bacteria and aloe at all and contains a mugwort component was used as Comparative Example 2, lactic acid bacteria, An anion patch (product name: Dasoni) containing no aloe and containing natural minerals was used as Comparative Example 3.

[ Comparative example 4]

A commercially available lactic acid bacteria product (Cell Biotech, Duolock Gold) containing the composition of Table 4 was used.

ingredient Lactobacillus Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus rhamnosus, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium Bifidum Excipient Silicon dioxide, tricalcium phosphate, sodium carboxymethylcellulose, synthetic vitamins, etc. sweetener Citric Anhydride, Orange Flavor Powder, Milk Flavor Mixture, Orange Flavor Mixture, etc.

[ Experimental example 1] Lactobacillus growth

BCP agar medium was used as the culture medium. The surface of the pad on which each sample was applied was brought into contact with the medium to check whether the lactic acid bacteria were buried or not. Incubation was carried out at 37 degrees Celsius for 24 hours.

When lactic acid bacteria proliferate in a purple medium, it becomes yellow due to a change in acidity.

As a result of the experiment, as shown in FIG. 1, the pad of Comparative Example 1 and the pad of Comparative Example 2 showed pale purple color, so that lactic acid bacteria did not proliferate, but the pad of Example 1 containing lactic acid bacteria showed yellow color, and thus lactic acid bacteria were observed. Became.

[ Experimental example 2] Antibacterial effect against E. coli

EMB agar medium was used as the culture medium. Each sample coated pad was cut into a size of 2.5 X 2.5 cm, placed on a plate coated with E. coli, and observed whether a clear zone occurred around the pad. Incubation was carried out at 37 degrees Celsius for 24 hours.

When lactic acid bacteria proliferate in a purple medium, it becomes yellow due to a change in acidity.

As a result of the experiment, as shown in FIG. 2, E. coli all proliferated around the pads of Comparative Examples 1 to 3, but a clear zone where E. coli did not proliferate was observed around the pad of Example 1 containing both lactic acid bacteria natural liposomes and aloe natural liposomes. It was found that it has antibacterial activity against E. coli.

[ Experimental example 3] E. coli growth inhibition effect

EMB agar medium was used as the culture medium. After coating the E. coli culture solution on the surface of the pad to which each sample was applied, and incubating for 24 hours, the degree of proliferation was observed by dipping the EMB agar medium to see how much E. coli proliferated on the pad surface. Incubation was carried out at 37 degrees Celsius for 24 hours.

As a result of the experiment, as shown in FIG. 3, the pad of Comparative Example 1 had a high density of E. coli, and the pad of Comparative Example 3 did not inhibit the growth of E. coli and proliferated. On the other hand, the pad of Example 1 containing both lactic acid bacteria natural liposomes and aloe natural liposomes was found to completely inhibit E. coli proliferation because no green light was observed in the medium.

[ Experimental example 4] Deodorizing effect

After 2 hours after injecting ammonia gas into the chamber in which each sample was placed, air was sucked into the ammonia detector tube and the residual gas amount was measured.

The amount reduced compared to the initial gas concentration was expressed in %, and this was expressed as the deodorization rate (%).

The results are shown in Table 5 below.

Control Pad of Example 1 Pad of Comparative Example 1 Concentration (ppm) 600 50 470 Deodorization rate (%) 0 91.6 21.6

As shown in Table 5, the pad of Example 1 showed a deodorizing effect of 91.6%, and was found to be remarkably superior to the pad of Comparative Example 1.

[ Experimental example 5] Experiment using intestinal epithelial cells (in vitro)

The adhesion of lactic acid bacteria and harmful bacteria was measured using human intestinal epithelial cells (Caco-2).

After dispensing 10 ⁵ cells/well of Caco-2 cells into microplates (24 wells) using serum medium (EMEM + 10% FBS + 1% Pen/strep), the cells are removed in an incubator at 37℃ and 5% CO ₂ . It was incubated until a monolayer was formed.

Lactobacillus fermentum (Lactobacillus fermentum) Miev L1106 (KCTC 12082BP) is a lactic acid bacteria, was prepared by subculture three times using MRS medium.

Escherichia coli (KCTC 1041) is a harmful bacteria, and was prepared by subculture 3 times using TSB (Trypticase soy broth) medium.

After removing the medium of each cell, it was washed 4 times with 500 ul of PBS. Each cell was treated with an antibiotic-free medium (EMEM + 10% FBS) as a negative control group, and the lactic acid bacteria composition of Example 2 and the lactic acid bacteria product of Comparative Example 4 were diluted in an antibiotic-free medium (EMEM + 10% FBS) as an experimental group, respectively. Processed. Thereafter, each cell was diluted with Lactobacillus fermentum at ¹ × ^{10 9} cfu/ml in serum medium, or E. coli diluted at 1× ^{10 9} cfu/ml in PBS and treated and incubated for 2 hours in an incubator at 37°C and 5% CO ₂ I did. After removing the medium of each cell, it was washed three times with PBS for 3 minutes. Each cell was detached with 0.2% trypsin-EDTA, and plated on MRS agar medium (Lactobacillus permentum treatment) or Mac Conkey agar medium (E. coli treatment) by continuous dilution using peptone water. This was cultured in a 37° C., 5% CO ₂ incubator for 24 hours and then the number of bacteria was measured, and the results are shown in Table 6 below.

Negative control Example 2 Comparative Example 4 Lactobacillus (cfu/ml) 0.73x10 ⁵ 15.5x10 ⁵ 0.82x10 ⁵ Coli (cfu/ml) 24.5x10 ⁵ 7.1x10 ⁵ 36x10 ⁵

As shown in Table 6, when the lactic acid bacteria composition of Example 2 was treated, the number of lactic acid bacteria adhering to the cells was greater and the number of E. coli was smaller than that of the conventional lactic acid bacteria product of Comparative Example 4.

Claims (19)

(a) lactic acid bacteria as core; (b) a first shell layer surrounding the core and comprising a natural polysaccharide; (c) a second shell layer surrounding the first shell layer and comprising a first natural emulsifier and a natural protein; And (d) surrounding the second shell layer, and including a third shell layer containing a natural cryoprotectant, and
A liposome constituent material containing at least one second natural emulsifier, a naturally derived solvent, a natural coating agent and a natural preservative; And aloe powder as a trapping material, wherein the natural emulsifier, the naturally derived solvent, and aloe-containing natural liposome formed by mixing the aloe powder by ultrasonic treatment.
Patch for a super absorbent polymer comprising a. According to claim 1, wherein the lactic acid bacteria-containing natural liposome and the aloe-containing natural liposome in a weight ratio of 1:0.5 ~ 1:2 for a superabsorbent resin patch comprising. The patch for super absorbent polymer according to claim 1, wherein the third shell layer further comprises a natural preservative. The method of claim 1, wherein the lactic acid bacteria are Lactobacillus acidophilus, Lactobacillus bulgaricus, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus fermentum, Li (Lactobacillus gasseri), Lactobacillus helveticus (Lactobacillus helveticus), Lactobacillus paracasei (Lactobacillus paracasei), Lactobacillus plantarum (Lactobacillus plantarum), Lactobacillus rhamnosus (Lactobacillus teriaceae, Lactobacillus r. reuteri), Lactobacillus salivarius, Lactococcus lactis, Streptococcus thermophilus, Bifidobacterium animalis ssp.lactis, Bifidobacterium High absorbency, one or more selected from the group consisting of Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium longum, and Enterococcus faecalis Patch for paper. The method of claim 1, wherein the natural polysaccharide is Arabic gum, Guar gum, Locust bean gum, Tamarind gum, Tara gum, Xanthan gum (Xanthan gum) and carrageenan (Carrageenan) at least one selected from the group consisting of a patch for a superabsorbent resin. The method of claim 1, wherein the first natural emulsifier and the second natural emulsifier are the same or different, and phosphatidylcholine, lysophosphatidylcholine, phosphatidylethanol amine, phosphatidylic acid, phosphatidylserine, phosphatidylglycerol, phosphatidylinositol, and hydrogenated products thereof. At least one super absorbent polymer patch selected from the group consisting of. The patch for superabsorbent polymer according to claim 1, wherein the natural protein is a milk-derived protein and/or a soy-derived protein. The patch for super absorbent polymer according to claim 1, wherein the natural cryoprotectant is at least one selected from the group consisting of skim milk powder, maltodextrin, and glycerin. The method of claim 1, wherein the lactic acid bacteria-containing natural liposome is based on the total weight, lactic acid bacteria 1 to 20% by weight, natural polysaccharide 0.5 to 5% by weight, the first natural emulsifier 5 to 30% by weight, natural protein 0.5 to 10% by weight And a patch for a super absorbent polymer comprising 45 to 75% by weight of a natural cryoprotectant. According to claim 3, wherein the natural preservative is one or more selected from the group consisting of citrus extract, gosam extract, citron extract, eucalyptus extract, grapefruit extract, cloves extract, and cranberry extract. The method of claim 3, wherein the super absorbent polymer patch is based on the total weight, based on the total weight, lactic acid bacteria 1 to 20% by weight, natural polysaccharide 0.5 to 5% by weight, the first natural emulsifier 5 to 30% by weight, natural protein 0.5 to 10% by weight %, 40 to 70% by weight of a natural cryoprotectant, and 5 to 15% by weight of a natural preservative. (i) a first step of ultrasonic treatment by adding a first natural emulsifier to a solvent of 40 to 75°C;
(ii) a second step of adding and mixing natural proteins and natural polysaccharides in a solvent of 40 to 75°C, and then adding and stirring lactic acid bacteria;
(iii) a third step of mixing the product obtained in the first step, the product obtained in the second step, and a natural cryoprotectant; And
(iv) A method for producing a patch for a super absorbent polymer according to claim 1, comprising the fourth step of adding a natural preservative to the product obtained in the third step. The method of claim 12, wherein the solvent is at least one selected from the group consisting of distilled water, naturally derived butylene glycol, propanediol, glycerin, and fermented alcohol. The method of claim 12, wherein in the second step, lactic acid bacteria are added to a solvent of less than 50°C. (i) a first step of adding and mixing natural polysaccharides in a solvent of 40 to 75°C, and then adding and stirring lactic acid bacteria;
(ii) a second step of ultrasonic treatment by adding a first natural emulsifier to a solvent at 40 to 75°C;
(iii) a third step of mixing by adding natural protein to a solvent at 40 to 75°C;
(iv) A method for producing a patch for a superabsorbent polymer according to claim 1, comprising a fourth step of mixing the product obtained in the first step, the product obtained in the second step, the product obtained in the third step, and a natural cryoprotectant. The method of claim 15, further comprising a fifth step of further adding and mixing a natural preservative after the fourth step. A hygiene product comprising the patch for a super absorbent polymer of claim 1. A diaper comprising the patch for a super absorbent polymer of claim 1. A sanitary napkin comprising the patch for a super absorbent polymer of claim 1.

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