KR102185922B1 - Lateral Flow Assay Strip for Preconcentration Fabricated by Infiltration Process - Google Patents

Abstract

In the present embodiments, a sample pad is immersed in a selective ion permeation solution and heat-treated to form a selective ion permeable region in a portion of the sample pad, thereby straightening the movement path of the analyte and an ion-deficient region and a concentration region having a thickness equal to the thickness of the sample pad. It provides a lateral flow analysis strip capable of forming.

Description

Lateral Flow Assay Strip for Preconcentration Fabricated by Infiltration Process}

The technical field to which this embodiment pertains to lateral flow analysis strips.

The content described in this section merely provides background information on the present embodiment and does not constitute the prior art.

Modern medicine does not simply extend the lifespan, but aims to realize the prolongation of a healthy longevity. Therefore, future medicine is not focused on therapeutic medicine, but the paradigm is changing to implement the 3P of Preventive Medicine, Predictive Medicine, and Personalized Medicine. In order to realize this in detail, early detection and early treatment of diseases have become very important means, and studies on biomarkers as a means for this are very active.

Biomarkers are markers that can distinguish normal or pathological conditions, predict treatment response, and measure objectively. As biomarkers, nucleic acids (DNA, RNA), proteins, lipids, metabolites, and the like are used. In other words, simple substances such as blood glucose for diagnosis of diabetes, genes such as BCR-ABL gene fusion of chronic myelogenous leukemia, which is the treatment target of Gleevec, are all biomarkers and are biomarkers that are actually used in clinical practice.

By analyzing nucleic acids or proteins, the degree of expression and progression of the disease can be determined. Technology and devices for protein analysis have a problem that is difficult to popularize because it is difficult and relatively expensive to manufacture devices by using nanotechnology. In addition, there is a disadvantage in that the protein analysis device requires a high-sensitivity sensor or it is difficult to accurately analyze a small amount of a sample. A typical method for detecting nucleic acids or proteins is a lateral flow analysis method using a chromatography method. This lateral flow analysis method is used in various fields such as pregnancy diagnosis.

The pregnancy diagnostic kit uses a monoclonal antibody that binds to the chorionic gonadotropin (HCG). A chromogenic substance is attached to the HCG antibody, which is a monoclonal antibody that responds only to HCG. HCG in urine binds to the HCG antibody of the pregnancy diagnostic kit and then moves, but in the pregnancy display window, the HCG complex and the antibody that binds to the HCG are bound and a red band appears, and in the end of the test window, the HCG antibody and HCG antibody with a chromogenic substance The antibody that binds to is bound and a red band appears.

HCG is usually easy to detect due to its high concentration, but other biomarkers have a low concentration, making it difficult to detect.

1 is a diagram illustrating a lateral flow analysis strip with a selective ion permeable membrane attached to a sample pad, and FIG. 2 is a diagram illustrating a state in which an electric field is applied to a selective ion permeable membrane attached to a sample pad.

1 and 2, when operating in a manner in which the selective ion exchange membranes 130, 131, and 132 are in contact with the top of the sample pad 120, ion concentration polarization (ICP) and ion deficiency ( Since the depletion phenomenon occurs at the contact surface between the selective ion exchange membrane 130 and the sample pad 120, the depletion layers 135 and 136 are not formed to cover the entire thickness of the sample pad 120, so that the sample pad ( There is a problem that the size of the concentration region 138 formed in 120) is small and it is difficult to concentrate the analyte to a high concentration. When a surfactant is present in a sample during the process of concentrating a specific analyte, since the surfactant suppresses the occurrence of ICP, there is a limit to concentrating the analyte to a high concentration.

Korean Patent Publication No. 10-2018-0056342 (published on May 28, 2018)

Embodiments of the present invention straighten the movement path of the analyte by immersing a sample pad in a selective ion permeation solution and heat treatment to form a selective ion permeable region in a portion of the sample pad, rather than attaching the selective ion permeable membrane to the sample pad. The main object of the present invention is to form an ion-deficient region and a concentration region having an analyte moving over the entire top and bottom of the sample pad and having a thickness equal to the thickness of the sample pad.

Still other objects, not specified, of the present invention may be additionally considered within the range that can be easily deduced from the following detailed description and effects thereof.

According to an aspect of the present embodiment, in the lateral flow analysis method using a lateral flow analysis strip for measuring an analyte in a sample, a sample pad is immersed in a selective ion permeation solution and heat-treated to form a selective ion permeable region in a portion of the sample pad. Forming, injecting the sample into the sample pad, concentrating the analyte by applying an electric field to the sample pad, and connecting a composite pad or a test pad to the sample pad to obtain the concentrated analyte It provides a lateral flow analysis method comprising the step of moving to the composite pad or the test pad.

According to another aspect of the present embodiment, in a lateral flow analysis strip for measuring an analyte in a sample, the support is connected to the support, a sample pad having a predetermined thickness for receiving the sample, and the support is connected, It comprises a test pad including a trap for capturing the analyte, and forms a structure in which the flow path of the analyte between the sample pad and the test pad is blocked, and the sample pad includes a selective ion permeable region, the It provides a lateral flow analysis strip, characterized in that by applying an electric field to the selective ion transmission region to form an ion-deficient region having a thickness equal to the thickness of the sample pad.

According to another aspect of the present embodiment, in a lateral flow analysis strip for measuring an analyte in a sample, a support, a sample pad connected to the support, and having a preset thickness for receiving the sample, is connected to the support, A test pad including a trap for capturing the analyte, a case for accommodating the lateral flow analysis strip, and the support, the sample pad, the test pad, or the case connected to the sample pad and the test pad. And a state conversion unit for blocking or connecting the flow path of the analyte, and the sample pad includes a selective ion-permeable region, and an ion-deficient region having a thickness equal to the thickness of the sample pad is formed by applying an electric field to the selective ion-permeable region. It provides a lateral flow analysis strip, characterized in that to form.

As described above, according to the embodiments of the present invention, a sample pad is immersed in a selective ion permeable solution and heat treated, rather than a method in which a selective ion permeable membrane is attached to the sample pad, thereby forming a selective ion permeable region in a part of the sample pad. In addition, there is an effect of straightening the movement path of the analyte and forming an ion-deficient region and a concentration region having a thickness equal to the thickness of the sample pad.

Even if it is an effect not explicitly mentioned herein, the effect described in the following specification expected by the technical features of the present invention and the provisional effect thereof are treated as described in the specification of the present invention.

1 is a diagram illustrating a lateral flow analysis strip with a selective ion permeable membrane attached to a sample pad.
2 is a diagram illustrating a state in which an electric field is applied to a selective ion permeable membrane attached to a sample pad.
3 is a diagram illustrating a lateral flow analysis strip having a selective ion permeable region on the side of a sample pad according to an embodiment of the present invention.
4 is a diagram illustrating a state in which an electric field is applied to a selective ion permeable region formed on a side surface of a sample pad of a lateral flow analysis strip according to an embodiment of the present invention.
5 and 6 are diagrams illustrating a structure of a sample pad of a lateral flow analysis strip according to embodiments of the present invention.
7 is a diagram illustrating a structure of a state changer of a lateral flow analysis strip according to embodiments of the present invention.
8 is a flowchart illustrating a lateral flow analysis method using a lateral flow analysis strip according to another embodiment of the present invention.
9 is a diagram illustrating a step of forming a selective ion permeable region in a lateral flow analysis method using a lateral flow analysis strip according to another embodiment of the present invention.
10 to 13 are diagrams illustrating results of concentration of an analyte using a lateral flow analysis strip according to embodiments of the present invention.

Hereinafter, in describing the present invention, when it is determined that the subject matter of the present invention may be unnecessarily obscured as matters apparent to those skilled in the art with respect to known functions related to the present invention, a detailed description thereof will be omitted and some embodiments of the present invention will be described. It will be described in detail through exemplary drawings.

3 is a diagram illustrating a lateral flow analysis strip having a selective ion permeation region on the side of the sample pad, and FIG. 4 is a diagram illustrating a state in which an electric field is applied to the selective ion transmission region formed on the side of the sample pad of the lateral flow analysis strip. to be.

Referring to FIG. 3, the lateral flow analysis strip includes a sample pad 120, a composite pad 150, a test pad 160, and an absorbent pad 170.

The sample pad 120, the test pad 160, and the absorption pad 170 may be fixed to the support 110, and the test pad 160 may serve as the support 110.

The sample pad 120 is a pad for injecting a sample to be detected.

The composite pad 150 is a pad including a detector coupled to a color developing material. The detector binds to the analyte in the sample.

The test pad 160 includes a detection area 162 and a control area 164, indicates whether an analyte is present in the sample, and indicates whether the sample is moving.

The absorption pad 170 is a pad that absorbs a sample by using a capillary phenomenon.

The pads may be implemented with a porous medium that responds to the capillary phenomenon. The medium includes, but is not limited to, cellulose, nitrocellulose, polyethersulfone, polyvinylidene, fluoride, nylon, and polytetrafluoroethylene, which may be used for lateral flow. The medium can be used alone or in combination with other materials.

The lateral flow analysis strip 100 includes a selective ion permeable region 121 for concentrating the low concentration biomarker. An electrode may be attached to the selective ion transmission region 121. The selective ion permeation region 121 for concentrating the sample may be implemented with Nafion, polystyrene sulfonate (PSS), or polyallylamine hydrochloride (PAH).

The selective ion transmission region 121 is connected to a power source. A thin film electrode may be provided in the selective ion transmitting region 121 to be connected to an external power source. A negative electrode, a positive electrode, or a ground is connected to the two selective ion transmission regions 121 and 122 through a wire, so that a potential difference is generated at both ends.

The sample pad 110 acts like a channel. The channel in which the selective ion transmission region is formed serves as a kind of nano filter that selects and transmits protons. For example, when the selective ion-permeable material is Nafion, H+ ions are selectively and rapidly permeated due to SO3- in the chemical structure of Nafion by hopping and vehicle mechanisms. Therefore, it is possible to efficiently concentrate protein substances to be analyzed in a specific region of the channel through a selective ion permeable material such as Nafion in a very fast time.

The sample kit may be implemented to generate an ICP phenomenon using a microporous material (eg, hydrogel). It may be implemented as a microporous transmissive region made of natural or artificial materials. Here, the fine pores may be pore diameter>10nm and pore volume>0.6 mL/g, but these are only examples and are not limited thereto, and fine pores of an appropriate size may be used according to the design to be implemented.

Microporous material (eg, nafion, etc.) is infiltrated into the sample pad. A selective ion permeable region may be created in the sample pad by infiltrating a liquid microporous material into a sample pad (eg, paper, etc.) and performing heat treatment.

5 and 6 are diagrams illustrating the structure of a sample pad of a lateral flow analysis strip. FIG. 5 shows a sample pad in which two selective ion-permeable regions are formed, and FIG. 6 shows a sample pad in which a selective ion-permeable membrane is attached to a sample pad in which one selective ion-permeable region is formed.

The analyte is concentrated using a depletion force generated in the selective ion permeation region and a flow of fluid from the sample pad to the absorbent pad.

The sample pad, that is, the channel, may be formed of a porous membrane with other types of materials capable of forming passive capillary force. In addition, a case where the selective ion permeable regions 121 and 122 are formed in two from the side will be described as an example.

The sample pad 120 includes a first selective ion transmitting region 121 formed to have a thickness equal to the thickness of one end of the sample pad and a second selective ion transmitting region 122 formed to have a thickness equal to the thickness of the other end of the sample pad .

In the sample pad 120, the first selective ion permeable region 121 and the second selective ion permeable region 122 are positioned to face each other, and the first selective ion permeable region 121 and the second selective ion permeable region 122 ) Are located in opposite directions with respect to the ion-deficient regions 125 and 126.

At this time, when a voltage difference occurs by applying power to the selective ion permeable regions 121 and 122 disposed at both ends, the ions present in the fluid are drawn toward the electrode opposite to the electrical property of each ion by an electric field according to the voltage difference. . As the ions move in the channel according to their electrical properties, the fluid particles are guided together by the viscous force. The overall fluid flow occurs, and the movement of the fluid is called electro-osmosis flow (EOF), and the movement of ions is called electrophoresis (EP).

The characteristics of these electrophoresis (Capillary Electrophoresis) and electro-osmosis (Electro-Osmosis) are different in the vicinity of the channel embodied as a selective ion permeation region, and ion concentration polarization (ICP) occurs. Accordingly, in the reaction region of the channel, ion depletion occurs at the cathode side and ion enrichment occurs at the anode side. At this time, the depletion zone (125, 126) acts as a kind of electric barrier to the analyte carrying a charge due to the depleted low ion concentration and the corresponding high electric field. As a result, the analyte does not pass through the deficient region and is concentrated before it. The analyte is concentrated very quickly in front of the deficient region in the channel. Since the size of the depleted region due to ion concentration polarization expands as the ion concentration polarization of the sample proceeds, the analyte forms a concentration region 128 in the center region of the channel.

The lateral flow analysis method is not limited to the antibody-antigen reaction, and the binding site (ligand) referred to herein is a protein ligand, a nucleic acid (DNA or RNA), in various analytes, the binding site of the molecular sequence, etc. And the specific binding material includes all biomolecules including proteins that can selectively and specifically bind to the binding site, viral phage, nucleic acid molecule aptamer, hapten (DNP), etc. Furthermore, it is not limited to the description.

The selective ion transmission regions 121 and 122 are formed at both ends of the sample pad 120 so as to cross the channel. The selective ion-permeable material forming the selective ion-permeable region refers to a material that binds well with and attracts each other, but does not bind and attracts other ions. The selective ion permeable material can be, for example, nafion.

Referring to FIG. 6, a sample pad having a selective ion permeable region 121 formed at one end may include a selective ion permeable layer 123 attached to one surface of the sample pad and an electrode connected to the selective ion permeable layer.

7 is a diagram illustrating a structure of a state transition part of a lateral flow analysis strip.

The lateral flow analysis strip transitions from (i) a first state that does not form an analyte flow path between the sample pad and the test pad, and (ii) transitions to a second state that forms an analyte flow path between the sample pad and the test pad. Includes wealth. The state switching unit may be integrally formed with the composite pad.

The state switching unit is formed in a structure in which the complex pad or test pad is separated when the analyte is concentrated, or the complex pad or the test pad is connected when the analyte is detected. The state switching unit is operated by pressing, sliding, turning, seesaw, removing the blocking film, or a combination thereof.

7(a), (b), and (c) illustrate a structure for connecting a composite pad or a test pad to a sample pad, and FIG. 7(d) illustrates a state switching unit operated in a pressing manner, and FIG. 7(e) and (f) illustrate a state switching unit operated in a slide manner, FIG. 7(g) illustrates a state switching unit operated in a rotation or seesaw method, and FIG. 7(h) is a blocking film removed. It illustrates a state transition unit that operates in a manner.

Referring to Figure 7 (a), the sample pad is connected to the support, and accommodates a sample. When power is applied to the selective ion transmitting region formed on the sample pad, the concentrated region 222a is formed in the center of the sample pad. The test pad is connected to the support and contains a trap to capture the analyte. The absorbent pad is connected to the test pad to absorb the sample by capillary action. The moving speed of the sample is different depending on the shape and material of the absorbent pad. The absorbent pad is manufactured by adjusting the length or absorbent capacity. The state switching unit is formed to be spaced apart from the sample pad by a predetermined distance so as not to contact the sample pad.

Referring to FIG. 7B, the state conversion unit may be connected to the test pad. Referring to (b) of FIG. 7, the state switching unit may be connected to the support.

Referring to FIG. 7C, the lateral flow analysis strip may include a support, a sample pad, a test pad, a state conversion unit, and a case.

The sample pad is connected to the support and has a predetermined thickness for receiving the sample. The test pad is connected to the support and contains a trap to capture the analyte. The case is formed to receive the lateral flow analysis strip.

The state conversion unit is connected to the support, the sample pad, the test pad, or the case, and blocks or connects the flow path of the analyte between the sample pad and the test pad.

The state conversion unit may be attached to a case or the like, and is not limited by a specific attachment method. For example, when the state conversion unit is attached to the case and pressure is applied to the case, the state conversion unit may be manufactured to contact the lateral flow analysis strip.

The sample pad includes a selective ion-transmitting region, and an ion-deficient region having a thickness equal to the thickness of the sample pad is formed by applying an electric field to the selective ion-transmitting region.

Referring to FIG. 7, the state conversion unit is converted from a first state to a second state. The first state is a state not in contact with the sample pad, and the second state is a state in which a flow path of an analyte is formed between the sample pad and the test pad by contacting the sample pad. The state conversion unit may contact the concentrated region of the sample pad. The concentrated analyte is transferred to the test pad through the state transition unit.

The lateral flow analysis strip contains a composite pad. The composite pad is connected to the test pad and includes a combination of a detector and an indicator that binds to the analyte to form a complex. The composite pad and the state conversion unit may be integrally formed.

Indicators are substances that generate signals so that they can be detected by the naked eye or by using a sensor. The indicator may be a color developing material, for example, gold nanoparticles, but is not limited thereto, and a suitable material may be used depending on the design to be implemented.

The test pad includes at least one of a detection area and a control area. The detection region includes a first capturer that captures the complex. That is, the detection region detects whether an analyte is present in the sample. The control region contains a second trap that captures the uncomplexed complex or detector. That is, the control region checks whether the sample has moved regardless of the presence or absence of an analyte.

A reaction that forms a complex in a composite pad or captures it in a test pad may be formed by a physical reaction, a chemical reaction, a biological reaction, or a combination thereof. For example, it may be an antigen-antibody reaction.

In the composite pad, an indicator-detector conjugate is located, and the analyte passes through the complex pad to form an indicator-detector-analyte complex. The shifted indicator-detector-analyte complex is captured by the first capturer in the detection region.

8 is a flowchart illustrating a method of analyzing a lateral flow using a lateral flow analysis strip according to another embodiment of the present invention, and FIG. 9 is a view illustrating a step of forming a selective ion permeable region in the lateral flow analysis method. It is assumed that the lateral flow analysis strip manufacturing process is performed by means of a manufacturing apparatus.

In the lateral flow analysis method using a lateral flow analysis strip for measuring an analyte in a sample, a sample pad is immersed in a selective ion permeation solution and heat-treated to form a selective ion-permeable region in a part of the sample pad (S11), the sample Injecting the sample into a pad (S12), concentrating the analyte by applying an electric field to the sample pad (S13), and connecting a composite pad or a test pad to the sample pad to transfer the concentrated analyte to the And moving to the composite pad or the test pad (S14).

Referring to FIG. 9A, in the step of forming a selective ion-permeable region on a part of the sample pad (S11), the selective ion-permeable solution (resin) is absorbed at one end of the sample pad to form a first selective ion-permeable region. Then, the selective ion permeable solution may be absorbed to the other end of the sample pad to form a second selective ion permeable region.

Referring to (b) of FIG. 9, the step of forming a selective ion permeable region on a part of the sample pad (S11) includes a first selective ion permeable region and a second selective ion permeable region in a temperature range of 50°C to 100°C. Heat treatment can be performed for 10 minutes to 2 hours at. Preferably, it may be heat-treated for 30 minutes at a temperature range of 80°C.

10 to 13 are diagrams illustrating results of concentration of an analyte using a lateral flow analysis strip according to embodiments of the present invention.

FIG. 10 is a result of concentrating an analyte in a structure in which a selective ion permeable membrane is attached to a sample pad, and FIG. 11 is a result of concentrating an analyte in a sample pad having a selective ion permeable region on one side of the sample pad. And FIG. 12 shows the results of concentration of analytes in a sample pad having a selective ion permeable region on one side of the sample pad and a sample pad having selective ion permeable regions on both sides of the sample pad.

Referring to FIG. 10, it can be observed that local ICP and depletion occur only on the contact surface between Nafion and the sample pad. That is, in the structure implemented by attaching Nafion and the sample pad, the depletion region and the concentration region are not formed over the entire thickness of the sample pad. In the structure where Nafion is attached to the top of the sample pad, a plurality of Nafions are spaced apart from each other in only one direction out of four directions, so that the force applied to the analyte acts in a'U' shape. It is difficult to cover the entire thickness of, and there is a limit to the concentration ratio.

Referring to FIG. 11, it can be observed that the selective ion transmission region is formed on one side of the sample pad to have a thickness equal to the thickness of the sample pad, so that ICP and depletion phenomena occur stably. In particular, when a buffer containing 1% surfactant is used, a concentration ratio close to about 10 times can be obtained even under 1X PBS conditions.

The sample pad with the selective ion permeation region has an optional ion permeable region and a permeable membrane installed in two directions close to one of the four directions of up, down, left and right, and the applied force acts in a'L' shape, so the entire thickness of one side of the sample pad It can cover and can straighten the movement path.

Referring to the movement paths of the concentrated analytes shown in Figs. 12 and 13, Nafion is wetted at both ends based on the electric field direction of the sample pad, and when an electric field is applied, it is more concentrated than when wetted to one end of the sample pad. It is possible to improve the ratio and ion concentration polarization phenomenon. Since the force applied by forming a selective ion permeable region apart from the four directions of the upper, lower, left, and right opposite sides acts in a'-' shape, the force can be concentrated over the entire thickness of both sides of the sample pad without dispersing the force. It is possible to minimize the movement path of the analyte.

According to the present embodiments, by configuring a matching table for voltage, electric field, interval, etc., the concentration amount can be appropriately adjusted according to the analyte. For example, Voltage-100 V, Sample buffer-1X PBS with 1% (v/v ration) Tween 20, Time-10 min, Sample volume-30 uL, Detection time-Within 3 minutes after connecting the conjugate pad, Electrode-Ag/ It can be set with AgCl electrode.

In the present embodiments, by separating a biomarker as an analyte through ion concentration polarization (ICP) and concentrating the analyte several times or more, there is an effect of detecting a biomarker that is not detected in a general strip. Biomarkers can be applied to proteins as well as hormones such as HCG (human 　chorionic 　gonadotropin). For example, by concentrating many biomarkers such as myocardial infarction biomarker-troponin, Alzheimer's biomarker-amyloid beta, breast cancer biomarker-HER2, the sensitivity can be improved.

The analyte can be visually confirmed using colorimetric analysis. After the reaction, the color can be analyzed and quantified using an image by photographing it with a mobile phone. The intensity of colors in the image can be measured or expressed as a hue value. Fluorescence analysis, that is, quantitative analysis is possible by applying a substance that emits fluorescence in the kit.

The present embodiments are for explaining the technical idea of the present embodiment, and the scope of the technical idea of the present embodiment is not limited by these embodiments. The scope of protection of this embodiment should be interpreted by the following claims, and all technical ideas within the scope equivalent thereto should be construed as being included in the scope of the present embodiment.

110: support 120: sample pad
121: first selective ion transmission region 122: second selective ion transmission region
125, 126: deficient region 128: enriched region
150: composite pad 160: test pad
162: detection area 164: contrast area
170: absorbent pad

Claims (15)

In the lateral flow analysis method using a lateral flow analysis strip for measuring an analyte in a sample,
Immersing a sample pad in a selective ion permeation solution and heat treatment to form a selective ion permeable region in a portion of the sample pad;
Injecting the sample into the sample pad;
Concentrating the analyte by applying an electric field to the sample pad; And
Connecting a composite pad or a test pad to the sample pad to move the concentrated analyte to the composite pad or the test pad,
Forming a selective ion permeable region on a portion of the sample pad,
The selective ion permeable solution is absorbed at one end of the sample pad to form a first selective ion permeable region, and the selective ion permeable solution is absorbed at the other end of the sample pad to form a second selective ion permeable region,
The sample pad forms an ion-deficient region having a thickness equal to the thickness of the sample pad by applying an electric field to the first selective ion transmission region and the second selective ion transmission region,
The first selective ion transmission region and the second selective ion transmission region are located opposite to each other, and the first selective ion transmission region and the second selective ion transmission region are located in opposite directions with respect to the ion deficient region,
The force applied to the ion-deficient region acts in a'-' shape instead of a'U' shape, concentrating the force over the entire thickness of both sides of the sample pad without dispersing the force, and according to the ion depletion region, the sample Lateral flow analysis method, characterized in that forming a concentrated region having a thickness equal to the thickness of the pad. delete The method of claim 1,
Forming a selective ion permeable region on a portion of the sample pad,
The lateral flow analysis method, characterized in that heat-treating the first selective ion permeable region and the second selective ion permeable region at a temperature range of 50°C to 100°C. In the lateral flow analysis strip for measuring the analyte in the sample,
support fixture;
A sample pad connected to the support and having a predetermined thickness to receive the sample; And
A test pad connected to the support and including a trapper for capturing the analyte,
Forming a structure in which the flow path of the analyte between the sample pad and the test pad is blocked,
The sample pad includes a first selective ion transmitting region formed to have a thickness equal to the thickness of one end of the sample pad and a second selective ion transmitting region formed to have a thickness equal to the thickness of the other end of the sample pad,
The sample pad forms an ion-deficient region having a thickness equal to the thickness of the sample pad by applying an electric field to the first selective ion transmission region and the second selective ion transmission region,
The first selective ion transmission region and the second selective ion transmission region are located opposite to each other, and the first selective ion transmission region and the second selective ion transmission region are located in opposite directions with respect to the ion deficient region,
The force applied to the ion-deficient region acts in a'-' shape instead of a'U' shape, concentrating the force over the entire thickness of both sides of the sample pad without dispersing the force, and according to the ion depletion region, the sample Lateral flow analysis strip, characterized in that to form a concentrated region having a thickness equal to the thickness of the pad. The method of claim 4,
Lateral flow analysis strip, characterized in that the analyte is concentrated 10 times or more than a structure in which a selective ion permeable membrane is attached to the sample pad. delete delete The method of claim 4,
A lateral flow analysis strip, characterized in that the analyte is concentrated 10 times or more than a structure in which a selective ion permeable membrane is attached to the sample pad even if a surfactant that suppresses ion concentration polarization is present in the sample. The method of claim 4,
(i) a state in which a flow path of the analyte is not formed between the sample pad and the test pad, and (ii) a state is switched to a second state in which a flow path of the analyte is formed between the sample pad and the test pad It further includes a transition,
The state switching unit is a lateral flow analysis strip, characterized in that the operation by pressing (Push), slide (Slide), rotation (Turn), seesaw (Seesaw), blocking film removal or a combination thereof. The method of claim 4,
The lateral flow analysis strip further comprises a complex pad connected to the test pad and comprising a combination of a detector and an indicator combined with the analyte to form a complex. The method of claim 4,
The test pad includes (i) a detection region including a first capturer for capturing the complex and/or (ii) a control region including a second capturer for capturing the combination or the detector that does not form the complex. Lateral flow analysis strip comprising a. The method of claim 4,
The lateral flow analysis strip further comprises an absorbent pad connected to the test pad and absorbing the sample by capillary action. In the lateral flow analysis strip for measuring the analyte in the sample,
support fixture;
A sample pad connected to the support and having a predetermined thickness to receive the sample;
A test pad connected to the support and including a trapper for capturing the analyte;
A case accommodating the lateral flow analysis strip; And
And a state conversion unit connected to the support, the sample pad, the test pad, or the case and blocking or connecting the flow path of the analyte between the sample pad and the test pad,
The sample pad includes a first selective ion transmitting region formed to have a thickness equal to the thickness of one end of the sample pad and a second selective ion transmitting region formed to have a thickness equal to the thickness of the other end of the sample pad,
The sample pad forms an ion-deficient region having a thickness equal to the thickness of the sample pad by applying an electric field to the first selective ion transmission region and the second selective ion transmission region,
The first selective ion transmission region and the second selective ion transmission region are located opposite to each other, and the first selective ion transmission region and the second selective ion transmission region are located in opposite directions with respect to the ion deficient region,
The force applied to the ion-deficient region acts in a'-' shape instead of a'U' shape, concentrating the force over the entire thickness of both sides of the sample pad without dispersing the force, and according to the ion depletion region, the sample Lateral flow analysis strip, characterized in that to form a concentrated region having a thickness equal to the thickness of the pad. The method of claim 13,
Lateral flow analysis strip, characterized in that the analyte is concentrated 10 times or more than a structure in which a selective ion permeable membrane is attached to the sample pad. delete

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