KR102164528B1 - Bio aerosol monitoring apparatus and method thereof - Google Patents

Abstract

According to the disclosed present invention, a bioaerosol monitoring apparatus includes: a collection unit collecting bioaerosol particles in air into a collection liquid; a first droplet discharge unit electrostatically spraying the collected liquid, which is obtained by collecting the particles, as a first droplet; a second droplet discharge unit electrostatically spraying a reagent capable of reacting with the particles in the first droplet as a second droplet; a reaction unit making the particles react with the reagent by mixing the first and second droplets with each other; and a measurement unit measuring the particles reacted with the reagent. A size of the first droplet discharged from the first droplet discharge unit is controlled so that the particles are individually included in the first droplet. According to such a configuration, the individual particles react with the reagent, so that bioaerosol monitoring is accurately performed through a naked eye.

Description

Bio-aerosol monitoring device and its method TECHNICAL FIELD [BIO AEROSOL MONITORING APPARATUS AND METHOD THEREOF}

The present invention relates to a bioaerosol monitoring device and method thereof, and more specifically, by spraying particles in the air into individual droplets by electrostatic spraying using a Taylor cone and reacting with reagents, accurate monitoring with the naked eye The present invention relates to a bioaerosol monitoring device and a method thereof.

In general, airborne microorganisms, that is, bioaerosol measurement methods, include a culture method in which biological particles suspended in a sample gas are collected on a solid or liquid surface suitable for proliferation, and cultured in an appropriate temperature and humidity environment for a certain period of time to measure the trapped microorganisms A staining method in which the collected microorganisms are stained and measured using a fluorescence microscope is mainly used.

In recent years, ATP bioluminescence method using the principle that ATP (adenosine triphosphate) and luciferin/luciferase react to emit light, a series that requires ATP scavenging, ATP extraction, and luminescence measurement. By reducing the process of the process to about 30 minutes, rapid measurement work became possible.

On the other hand, such a general bioaerosol measurement method is difficult to measure suspended microorganisms in the air in real time, and requires a high skill level of a measurer capable of accurately performing each measurement step. In addition, airborne microbial measurement equipment is very expensive, so it is difficult for general users to easily use it.

Accordingly, in recent years, there is a trend that various studies that allow users to easily access and accurately measure in real time from various places, moving away from the existing limited bioaerosol measurement method, are continuously being conducted.

Korean Patent Publication No. 10-1912521 Japanese Patent Publication No. 4797652

An object of the present invention is to provide a bioaerosol monitoring device capable of accurately monitoring a bioaerosol with the naked eye by electrostatic spraying bioaerosol particles individually and reacting with a reagent.

Another object of the present invention is to provide a bioaerosol monitoring method capable of improving measurement accuracy by electrostatic spraying bioaerosol particles individually and reacting with reagents.

The bioaerosol monitoring apparatus according to the present invention for achieving the above object includes a collection unit that collects bioaerosol particles in the air as a collection liquid, and discharges a first droplet electrostatically spraying the collection liquid in which the particles are collected as a first droplet. Part, a second droplet discharge unit electrostatically spraying a reagent capable of reacting with the particles as a second droplet as the first droplet, a reaction unit for mutually reacting the particles and the reagent by mixing the first and second droplets, and And a measuring unit for measuring the particles reacted with the reagent, and the size of the first droplet discharged from the first droplet discharge unit is controlled so that the particles are individually included in the first droplet.

Further, it includes a classification unit for aerodynamically classifying the microbial particles in the air, and the particles may be classified from the classification unit and introduced into the collecting unit.

In addition, the first droplet discharge unit is provided with a particle inlet through which the collecting liquid in which the particles are collected is introduced, and a particle outlet extending in a longitudinal direction from the particle inlet to discharge the first droplet to the reaction unit. A first discharge cone having a shape of a Taylor cone, which is provided between the discharge body, the particle inlet and the particle discharge port, and discharges the collected liquid into the first droplet containing the individual particles by electrostatic spraying. A first control unit controlling opening and closing of the first discharge cone by controlling an electrostatic force greater than the surface tension of the collecting liquid to be applied to the first discharge cone to open the first discharge cone, and through the first discharge cone It may include a first oil supply unit supplying oil to the first droplet to maintain the state of the discharged first droplet.

In addition, the second droplet discharge unit includes a reagent inlet through which the reagent is introduced, a second discharge body extending in a longitudinal direction from the reagent inlet to discharge the second droplet to the reaction unit, and the reagent inlet And a second discharge cone having a Taylor cone shape for electrostatic spraying of the second droplet individually, and an electrostatic force greater than the surface tension of the reagent so that the second discharge cone is opened. 2 A second control unit that controls to be applied to the discharge cone to control opening and closing of the second discharge cone, and oil as the second droplet to maintain the state of the individual second droplets discharged through the second discharge cone. It may include a second oil supply to supply.

In addition, the first discharge cone may be controlled to be opened to a size of less than 1 μm by the first control unit so that the particles of less than 1 μm are individually included in the first droplet.

In addition, the flow rate of the collection liquid in which the particles are collected and the flow rate of the oil are supplied to the first discharge body at a ratio of 5 μL/Hour: 400 μL/Hour, and a positive electrode and a negative electrode, respectively, with the first discharge cone interposed therebetween. A voltage of 5500 to 6500 V may be applied to the first positive electrode and negative electrode applying bodies to which are applied, and 3 to 7% of surfactant may be included in the oil.

In addition, the opening size of the second discharge cone may be controlled to a size of less than 1 μm by the second control unit.

In addition, the reaction unit may provide a reaction path bent a plurality of times so that the first and second droplets are mixed with each other.

In addition, the reagent may include a fluorescent solution capable of reacting with microorganisms.

In addition, the reagent is dyed by reacting with the particles, and the measuring unit may measure the stained particles with the naked eye through a photosensor or a fluorescence microscope.

The bioaerosol monitoring apparatus according to a preferred embodiment of the present invention includes a first droplet discharge unit including a first Taylor cone electrostatically spraying a first droplet containing individual particles collected in a collection liquid, the A second droplet discharge unit including a second Taylor cone for electrostatically spraying a reagent capable of reacting with the particles as a first droplet as a second droplet, a reaction unit for mutually reacting with the first and second droplets, and a reaction with the reagent It includes a measuring unit for measuring one of the particles.

In addition, the first droplet discharge unit is provided with the first tailor cone, the first discharge body through which the collecting liquid is introduced, and applying a voltage through the first tailor cone, so as to be less than the surface tension of the collecting liquid. A first control unit that generates a large electrostatic force to control opening and closing of the first tailor cone, and a first oil supply unit that supplies oil to the first droplet discharged from the first tailor cone, and discharges the second droplet The part is provided with the second tailor cone, a second discharge body through which the reagent is introduced, and a voltage is applied between the second tailor cone to generate an electrostatic force greater than the surface tension of the reagent, and the second tailor A second control unit for controlling opening and closing of the cone, and a second oil supply unit supplying oil to the second droplet discharged from the second tailor cone may be included.

In addition, the first tailor cone may be controlled to be opened to a size of less than 1 μm by the first control unit so that the particles of less than 1 μm are individually included in the first droplet.

In addition, the flow rate of the collection liquid in which the particles are collected and the flow rate of the oil are supplied to the first discharge body at a ratio of 5 μL/Hour: 400 μL/Hour, and the positive electrode and the negative electrode, respectively, with the first tailor cone interposed therebetween. A voltage of 5500 to 6500 V may be applied to the first positive electrode and negative electrode applying bodies to which are applied, and 3 to 7% of surfactant may be included in the oil.

In addition, the reaction unit provides a reaction path in which the first and second droplets are mixed with each other so that the particles are stained by the reagent, and the measurement unit is stained with the naked eye through a photosensor or a fluorescence microscope. Particles can be measured.

The bioaerosol monitoring method according to a preferred embodiment of the present invention is a particle discharging step of electrostatically spraying the bioaerosol particles collected as a collection liquid into a first droplet individually included, and reacting with the particles toward the first droplet. A reagent discharge step of electrostatically spraying a reagent into a second droplet, a reaction step of mixing and reacting the reagent and particles, and a measurement step of measuring the particles reacted with the reagent.

In addition, the particle discharging step and the reagent discharging step may be performed simultaneously.

In addition, prior to the particle discharging step, a collecting step of collecting the particles in the air with the collecting liquid in at least one of an electrostatic precipitation method, an impinger method, or a wet cyclone method. It may include.

In addition, in the particle discharging step, the collecting liquid in which the particles are collected is introduced into the first discharging body, and the first discharging cone having a Taylor cone shape provided inside the first discharging body. Electrostatic spraying the first droplet containing particles individually, and a first oil supplying step of supplying oil to the first droplet, wherein the first discharge cone has an electrostatic force greater than the surface tension of the collecting solution. The opening can be controlled so that it can be applied and spray the first droplet.

In addition, the first discharge cone may be controlled to be opened to a size of less than 1 μm so that the particles less than 1 μm are individually included in the first droplet.

In addition, in the reagent discharging step, the reagent is introduced into the second discharging body, and the second droplet is electrostatically sprayed from a second discharging cone having a Taylor cone shape provided inside the second discharging body. And a second oil supply step of supplying oil to the second droplet, wherein the second discharge cone is opened to spray the second droplet by applying an electrostatic force greater than the surface tension of the reagent. Can be controlled.

In addition, in the reaction step, the reagent stains the particles, and in the measuring step, the particles stained by the reagent may be measured through a photosensor or a fluorescence microscope.

According to the present invention having the above configuration, first, particles such as bacteria floating in the air are individually electrostatically sprayed to react with a reagent, thereby enabling accurate bioaerosol measurement even with the naked eye.

Second, real-time monitoring becomes possible as accurate measurement of bioaerosol is possible.

Third, it is advantageous in reducing manufacturing cost as it is possible to measure bioaerosol with a simple structure.

1 is a block diagram schematically showing a bioaerosol monitoring device according to an embodiment of the present invention.
FIG. 2 is a schematic configuration diagram illustrating an example of a collecting unit illustrated in FIG. 1.
3 is a schematic configuration diagram illustrating a first droplet discharge unit, a second droplet discharge unit, a reaction unit, and a measurement unit shown in FIG. 1. And,
FIG. 4 is a schematic enlarged view of area V of FIG. 3.

Hereinafter, a preferred embodiment of the present invention will be described with reference to the accompanying drawings. However, the spirit of the present invention is not limited to such an embodiment, and the spirit of the present invention may be proposed differently by addition, change and deletion of components constituting the embodiment, but this is also included in the spirit of the invention. It becomes.

Referring to FIG. 1, a bioaerosol monitoring device 1 according to a preferred embodiment of the present invention includes a classification unit 10, a collection unit 20, a first droplet discharge unit 30, and a second droplet discharge unit ( 40), and a reaction unit 50 and a measurement unit 60.

The classification unit 10 aerodynamically classifies the microbial particles P in the air A. The classification unit 10 may use a virtual impactor provided with a micro channel for classifying particles P from the introduced air A according to their size. Since the particle (P) classification technology of the classification unit 10 using such a virtual impactor is not the gist of the present invention, detailed illustration and description will be omitted. In addition, a modified example in which the classification unit 10 is not provided and the microbial particles P in the air A are collected directly by the collecting unit 20 to be described later is also possible.

The collecting part 20 collects particles P floating in the air A. In this embodiment, it is exemplified that particles P such as bacteria and viruses having a relatively small size are collected among microbial particles P in the air A. For reference, in the present invention, the particles P classified by size through the classification unit 10 are exemplified as flowing into the collection unit 20, but are not limited thereto. That is, a modification in which the particles P in the air A are not classified according to the size is also possible without the collection unit 20 passing through the classification unit 10.

As shown in FIG. 2, the collecting unit 20 collects the bioaerosol particles P contained in the air A as a collecting liquid W. To this end, the collecting part 20 has an inlet 22 through which air A is introduced, and a collecting pipe 21 extending from the inlet 22 in the longitudinal direction and facing the outlet 23 through which it is formed. In addition, the collecting unit 20 includes first and second electrodes 24 and 25 which are disposed to face each other between the inlet 22 and the outlet 23 of the collecting pipe 21 to which voltage is applied, It is exemplified that the particles (P) are collected with the collecting liquid (W) by electrostatic precipitation. Here, the first electrode 24 has a tip shape, and the second electrode 25 facing the first electrode 24 has a plate shape, but is not limited thereto.

Particles (P) of the air (A) introduced into the inlet (22) of the collection tube (21) is generated between the first and second electrodes (24) (25), the second electrode (25) It is collected in the collection liquid (W) provided on the side. At this time, the collecting liquid W is introduced through the collecting liquid inlet 27 provided on the collecting liquid support 26 and then discharged through the collecting liquid outlet 28. For reference, it is exemplified that the collection liquid W contains pure water (DI-Water), and the aerosol particles P including bacteria are collected in the collection liquid W.

On the other hand, the method of collecting particles (P) in the air (A) containing microorganisms such as bacteria in the collection liquid (W) is not limited to the electrostatic precipitation method as shown in Figure 2, air (A) It can be transformed into any one of various collection methods capable of collecting the medium particles (P) in the liquid collection liquid (W). For example, it is natural that the collection unit 20 can be modified in any one of various collection methods such as an impinger and a wet cyclone.

The first droplet discharge unit 30 electro-sprays the particles P collected in the collection liquid W and discharges them individually as the first droplet D1. As shown in FIG. 3, the first droplet discharge part 30 includes a first discharge body 31, a first discharge cone 34, a first control part 35 and a first oil supply part 36. .

The first discharge body 31 extends in the longitudinal direction from the particle inlet 32 through which the particles P collected in the collecting liquid W by the collecting part 20 flow in, and the particles ( A particle discharge port 33 for discharging P) to the reaction unit 50 is provided. The particles P collected in the collecting liquid W are introduced into the first discharge body 31.

The first discharge cone 34 is provided between the particle inlet 32 and the particle outlet 33, and collects the particles P collected in the collecting liquid W through the particle inlet 32 as a single first droplet ( D1) has a Taylor cone shape to discharge. The cone-shaped end of the first discharge cone 34 is a kind of nozzle, and as shown in FIG. 4, when an electrostatic force that overcomes the surface tension is applied, It is electrostatically sprayed with the droplet D1. Here, the size of the first droplet D1 electrostatically sprayed from the first discharge cone 34 may be controlled through the control of the open range G of the first discharge cone 34.

The first controller 35 controls the opening and closing of the first discharge cone 34 by controlling the electrostatic force applied to the first discharge cone 34. The first control unit 35 includes a first positive electrode applying body 35a for applying a voltage of the positive electrode and a first negative electrode applying body 35b for applying a voltage of the negative electrode, with the first discharge cone 34 interposed therebetween. It is provided on the discharge body (31). The voltages applied to the first positive and negative electrodes 35a and 35b are controlled by the first control unit 35. Therefore, when the electrostatic force generated by the first control unit 35 is controlled to be greater than the surface tension of the collecting liquid W in which the particles P are collected, the cone end of the first discharge cone 34 is opened. The particles PW collected in the collection liquid W are electrostatically sprayed as the first droplet D1.

At this time, as shown in FIG. 4, the open range G of the end of the first discharge cone 34 can be adjusted by controlling the electrostatic force applied by the first control unit 35, so that the first discharge cone 34 It is possible to control the size of the first droplet D1 electrostatically sprayed from. In addition, the first control unit 35 may control the size of the first droplet D1 sprayed from the first discharge cone 34 by controlling the inclination angle formed by the first discharge cone 34.

More specifically, the first control unit 35 controls the opening range G of the first discharge cone 34 to be opened to less than 1 μm, thereby having a size of less than 1 μm through the first discharge cone 34. Particles (P) are sprayed with the first droplet (D1). At this time, as the first droplet D1 of less than 1 μm discharged from the first discharge cone 34 contains particles P, such as bacteria having a size of approximately 0.6 to 0.8 μm, individually, the first discharge cone ( Only the individual particles P are included in the first droplet D1 electrostatically sprayed through 34).

That is, as the size of the first droplet D1 sprayed through the first discharge cone 34 is 1 μm, microbial particles of 1 μm or more may be included in the first droplet D1, and a size of less than 1 μm Only bioaerosol particles (P), such as bacteria having a, can be sprayed with the first droplet (D1) together with the collection liquid (W). For reference, particles P may not be included in the first droplet D1 discharged through the first discharge cone 34. That is, microbial particles (P) are not included in the first droplet (D1) that has passed through the first discharge cone (34), and only pure water (DI Water), which is the collection liquid (W), will be discharged as the first droplet (D1). It could be.

The first oil supply unit 36 supplies oil (O) to maintain the state of the first droplet (D1) discharged through the first discharge cone (34). That is, the first oil supply unit 36 is a collection liquid (W) containing pure water in the oil (O), such as an emulsion in which one of two liquids that do not dissolve each other is dispersed in the state of small particles on the other. Oil (O) is supplied so that the shape of the first droplet (D1) of) is maintained. This first oil supply part 36 is directed toward the end of the first discharge cone 34 so that oil (O) can be directly supplied to the first droplet (D1) sprayed from the first discharge cone 34 Supply.

In this embodiment, the first oil supply unit 36 is provided in a pair so as to face each other toward the end of the first discharge cone 34 to supply the oil O, but it is natural that the first oil supply unit 36 is not limited thereto.

Meanwhile, the first anode and cathode applying bodies 35a and 35b of the first droplet discharging unit 30 described in this embodiment include ITO electrodes, and the first anode and cathode applying bodies 35a and 35b The spaced distance L between them is illustrated as being approximately 4mm. In addition, the diameter d of the particle inlet 32 is illustrated as being approximately 0.1 mm.

In addition, the required applied voltage for discharging the first droplet D1 through the first discharge cone 34 is approximately 4000V, and the following conditions can be applied to adjust the size of the first droplet D1 to 1㎛. have. That is, the flow rate of the collection liquid (W) in which the particles (P) flowing into the particle inlet 32 and the flow rate of the oil (O) are 5 μL/Hour: 400 μL/Hour to the first discharge body 31 It is supplied, and the voltage applied to the first positive and negative electrodes 35a and 35b is approximately 6000V, and the concentration of a surfactant such as Span 80 that may be included in the oil O may be approximately 3-7%. . Through these conditions, the first discharge cone 34 can discharge the first droplet D1 of 1 μm.

The second droplet discharge unit 40 electrostatically sprays the reagent I capable of reacting with the particles P toward the first droplet D1 sprayed from the first droplet discharge unit 30. Like the first droplet discharge unit 30 described above, the first droplet discharge unit 30 has a second discharge body 41, a second discharge cone 44, a second control unit 45, and a second oil supply unit ( 46).

For reference, the reagent (I) described in the present invention is exemplified as containing a fluorescent solution capable of fluorescently reacting with microorganisms in the ATP method, including luciferin/luciferase, but is not limited thereto. Of course.

The second discharge body 41 includes a reagent inlet 42 through which the reagent I is introduced, and a reagent outlet 43 extending longitudinally from the reagent inlet 42 to discharge the reagent I to the reaction unit 50. ) Is provided. At this time, the reagent outlet 43 of the second discharge body 41 is in communication with the particle discharge port 33 of the first discharge body 31, and the first and second discharge bodies 31 and 41 communicate with each other. The particle outlet 33 and the reagent outlet 43 are connected to the reaction unit 50. Therefore, the particle discharge port 33 and the reagent discharge port 43 have a type of "Y" shape separated from each other with respect to the reaction unit 50.

The second discharge cone 44 is provided between the reagent inlet 42 and the reagent outlet 43, and electrostatically sprays the reagent (I) introduced through the reagent inlet 42 to provide a product containing individual reagents (I). It has a Taylor cone shape that discharges as two droplets (D2).

The second control unit 45 controls the opening and closing of the second discharge cone 44 to control spraying of the second droplet D2 including the reagent I discharged through the second discharge cone 44. That is, the second control unit 45 includes the second positive electrode and negative electrode applying bodies 45a and 45b so that an electrostatic force greater than the surface tension of the reagent I is applied, like the first control unit 35 described above. The second anode and cathode applying bodies 45a and 45b are provided on the second discharge body 41 with the second discharge cone 44 interposed therebetween, so that the electrostatic force generated by the application of voltage is greater than the surface tension of the reagent I. By controlling to be large or small, the second discharge cone 44 is opened or closed.

Here, the second control unit 45 controls to electrostatically spray the reagent I with the second droplet D2 by opening the second discharge cone 44 to less than 1 μm, like the first control unit 35. However, it is not necessarily limited thereto, and it is natural that the open range and conditions of the second discharge cone 44 can be variously changed.

The second oil supply unit 46 supplies oil O to maintain the state of the second droplet D2 discharged through the second discharge cone 44. Like the first oil supply unit 36, the second oil supply unit 46 can directly supply the oil O to the second droplet D2 sprayed from the second discharge cone 44. Supply oil (O) toward the end of 44). In addition, the second oil supply unit 46 is provided in a pair so as to face each other toward the end of the second discharge cone 44 and is illustrated and illustrated as supplying the oil O, but is not necessarily limited thereto.

For reference, the second droplet discharging unit 40 is also controlled under the same conditions as the first droplet discharging unit 40 described above, so that the second discharging cone 44 is It is good to discharge.

The reaction unit 50 includes particles (P) included in the first and second droplets (D1) (D2) electrostatically sprayed individually through the first droplet discharge unit 30 and the second droplet discharge unit 40 Reagent (I) is mixed and reacted. This reaction unit 50 has a reaction path 51 bent a plurality of times, as shown in FIG. 3. Here, the first and second droplets D1 and D2 are introduced into the reaction path 51 of the reaction unit 50 through the mixing inlet 52 and mixed through the reaction path 51 to be reacted, followed by mixing. It is discharged through the discharge port 53.

For reference, the particles P in the first droplet D1 through the reaction path 51 react with the second droplet D2 including the reagent I to be dyed in the reaction droplet ID state. In addition, the first droplet D1 that does not contain the particles P does not react with the second droplet D2, thereby maintaining the state of the first droplet D1.

The measurement unit 60 measures the particles P reacted with the reagent I. That is, in the measurement unit 60, the first droplet D1 containing the particles P among the first droplet D1 and the second droplet D2 containing the reagent I are mutually The mixed and dyed reaction droplet (ID) is measured. This measuring unit 60, as shown in Figure 3, the reaction droplet (ID) and the first droplet (D1) is discharged from the reaction unit 50 and collected in the collection container 61 and the collection container 61 It includes a detecting means 62 for measuring the collected reaction droplet (ID).

The collection container 61 communicates with the mixing outlet 53 of the reaction unit 50. In the collection container 61, a reaction droplet ID that is dyed by reacting with the reagent I and a first droplet D1 that has not reacted with the reagent I because it does not contain particles P are collected. In addition, the oil (O) discharged from the first and second oil supply units 36 and 46 to maintain the droplet shape is also collected in the collection container 61.

The detection means 62 is exemplified as including at least one of equipment capable of detecting the reaction droplet ID with the naked eye such as a photosensor or a fluorescence microscope. In the present embodiment, as the reagent (I) is exemplified as containing luciferin/luciferase, the detection means 62 exemplifies the particles P that react with the reagent (I) in fluorescence as a fluorescence microscope.

A bioaerosol monitoring method using the bioaerosol monitoring device 1 according to the present invention having the above configuration will be described with reference to FIGS. 1 to 4.

The bioaerosol monitoring method includes a collection step, a particle discharge step, a reagent discharge step, a reaction step and a measurement step.

In the collection step, bioaerosol particles (P) such as bacteria in the air (A) are collected as a collection liquid (W). In this collecting step, after aerodynamically separating the particles (P) using the fractionating unit 10, the particles (P) are collected as a collecting liquid (W) as shown in FIG. 2.

In the particle discharging step, the particles P collected with the collection liquid W are electrostatically sprayed into a single first droplet D1. In this particle discharging step, as shown in FIGS. 3 and 4, the first discharging cone 34 having the shape of a tailor cone is applied to the collecting liquid (W) including the particles P introduced into the first discharging body 31. Electrostatic spraying is performed from the to the first droplet D1. At this time, the electrostatic force applied to the first discharge cone 34 is controlled by the first control unit 35, and the first droplet D1 is discharged to the oil O supplied from the first oil supply unit 36 at the same time. As a result, it flows into the reaction unit 50 while maintaining the droplet shape.

In the reagent discharge step, the electrostatic force applied to the reagent (I) to the second discharge cone 44 having a tailor cone shape is controlled by the second control unit 45, as shown in FIG. 3, so that the same as the second droplet D2. It is electrostatically sprayed in the form of droplets. At this time, the second droplet D2 is discharged and introduced into the reaction unit 50 while maintaining the droplet shape by the oil O supplied from the second oil supply unit 46.

For reference, it is preferable that the particle discharging step and the reagent discharging step are performed simultaneously, while maintaining the droplet shape by the oil (O), and flowing into the reaction unit 50 at the same time. However, the present invention is not limited thereto, and the reagent discharge step is performed after the particle discharge step, or the second droplet (D2) including the reagent (I) is electrostatically sprayed first in the reagent discharge step, and then the first drop ( A variant of electrostatic spraying D1) is also possible.

In the reaction step, as shown in FIG. 3, the first and second droplets D1 and D2 are introduced into the reaction path 51 bent a plurality of times, and are mixed and reacted while passing through the reaction path 51. At this time, the first droplet (D1) containing the particles (P) of the first droplet (D1) reacts with the reagent (I) of the second droplet (D2) and is dyed with the reaction droplet (ID), and the particles (P) As only the pure component, which is the collection liquid W, is contained in the first droplet D1 that does not contain, it does not react with the second droplet D2 and maintains the state of the first droplet D1.

In the measuring step, the reaction droplet (ID) that has undergone the reaction step is collected in the collecting container 61 of the measuring unit 60, and then measured by a detection means 62 such as a photosensor and an optical microscope. Accordingly, the distribution, number, and volume of the bioaerosol particles P contained in the air (A) are finally detected and monitored in real time.

As described above, although it has been described with reference to preferred embodiments of the present invention, those skilled in the art can variously modify and change the present invention without departing from the spirit and scope of the present invention described in the following claims. You will understand that you can do it.

1: bioaerosol monitoring device 10: classification unit
20: collection unit 30: first droplet discharge unit
31: first discharge body 34: first discharge cone
35: first control unit 36: first oil supply unit
40: second droplet discharge unit 41: second discharge body
44: second discharge cone 45: second control unit
46: second control unit 50: reaction unit
51: reaction path 60: measurement unit
P: Particle D1: First droplet
D2: second droplet ID: reaction droplet

Claims (22)

delete delete delete delete delete delete delete delete delete delete A first droplet discharging unit including a first Taylor cone for electrostatic spraying individually the first droplets each of which particles are collected in the collection liquid;
A second droplet discharge unit including a second tailor cone electrostatically spraying a reagent capable of reacting with the particles as a second droplet as the first droplet;
A reaction unit for mutually reacting with the first and second droplets; And
A measuring unit for measuring the particles reacted with the reagent;
Including,
The first and second droplet discharge units supply oil to the discharged first and second droplets, and the flow rate of the collection liquid containing the particles and the flow rate of the oil are 5 μL/Hour: 400 μL/Hour. Supplied to the first droplet discharge unit,
Bioaerosol monitoring device containing a surfactant in the oil. The method of claim 11,
The first droplet discharge unit,
A first discharge body provided with the first tailor cone and into which the collecting liquid flows;
A first controller configured to control opening/closing of the first tailor cone by applying a voltage across the first tailor cone to generate an electrostatic force greater than the surface tension of the collecting liquid; And
A first oil supply unit supplying the oil to the first droplet discharged from the first tailor cone;
Including,
The second droplet discharge unit,
A second discharge body provided with the second tailor cone and into which the reagent is introduced;
A second controller configured to control opening/closing of the second tailor cone by applying a voltage across the second tailor cone to generate an electrostatic force greater than the surface tension of the reagent; And
A second oil supply unit supplying the oil to the second droplet discharged from the second tailor cone;
Bioaerosol monitoring device comprising a. The method of claim 12,
The bioaerosol monitoring device is controlled to be opened to a size of less than 1 μm by the first controller so that the particles of less than 1 μm are individually included in the first droplet. The method of claim 13,
A voltage of 5500 ~ 6500V is applied to the first positive electrode and the negative electrode applicator for applying the positive and negative electrodes, respectively, with the first Taylor cone interposed therebetween,
Bioaerosol monitoring device containing 3 to 7% of the surfactant in the oil. The method of claim 11,
The reaction unit provides a reaction path bent a plurality of times so that the first and second droplets are mixed with each other so that the particles are dyed by the reagent,
The measuring unit bioaerosol monitoring device for measuring the particles dyed with the naked eye through a photosensor or a fluorescence microscope. Particle discharging step of electrostatically spraying the bioaerosol particles collected by the collection liquid into individual first droplets individually included;
A reagent discharge step of electrostatically spraying a reagent capable of reacting with the particles toward the first droplet as a second droplet;
A reaction step of mixing and reacting the reagent and the particles; And
A measuring step of measuring the particles reacted with the reagent;
Including,
In the particle discharging step and the reagent discharging step, oil is supplied to the first and second droplets, respectively, and the flow rate of the collection liquid containing the particles and the flow rate of the oil are supplied in a ratio of 5 μL/Hour: 400 μL/Hour. And, a bioaerosol monitoring method comprising a surfactant in the oil. The method of claim 16,
Bioaerosol monitoring method in which the particle discharge step and the reagent discharge step are performed simultaneously. The method of claim 16,
Prior to the particle discharging step, a collecting step of collecting the particles in the air in the collecting liquid by at least one of an electrostatic precipitation method, an impinger method, or a wet cyclone method;
Bioaerosol monitoring method comprising a. The method of claim 16,
The particle discharge step,
Introducing the collection liquid in which the particles are collected into a first discharge body;
Electrostatically spraying the first droplet including the particles individually from a first discharge cone having a Taylor cone shape provided inside the first discharge body; And
A first oil supply step of supplying the oil with the first droplet;
Including,
The bioaerosol monitoring method in which the opening of the first discharge cone is controlled to spray the first droplet by applying an electrostatic force greater than the surface tension of the collecting liquid. The method of claim 19,
Bioaerosol monitoring method in which the first discharge cone is controlled to open to a size of less than 1 μm so that the particles of less than 1 μm are individually included in the first droplet. The method of claim 16,
The reagent discharge step,
Introducing the reagent into a second discharge body;
Electrostatically spraying the second droplet from a second discharge cone having a Taylor cone shape provided inside the second discharge body; And
A second oil supply step of supplying the oil to the second droplet;
Including,
Bioaerosol monitoring method in which the opening of the second discharge cone is controlled to spray the second droplet by applying an electrostatic force greater than the surface tension of the reagent. The method of claim 16,
In the reaction step, the reagent stains the particles,
The measuring step is a bioaerosol monitoring method for measuring the particles stained by the reagent through a photosensor or a fluorescence microscope.

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