KR102153984B1 - The fiber for the pet clothing using the mineral composite - Google Patents
The fiber for the pet clothing using the mineral composite Download PDFInfo
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- KR102153984B1 KR102153984B1 KR1020190141623A KR20190141623A KR102153984B1 KR 102153984 B1 KR102153984 B1 KR 102153984B1 KR 1020190141623 A KR1020190141623 A KR 1020190141623A KR 20190141623 A KR20190141623 A KR 20190141623A KR 102153984 B1 KR102153984 B1 KR 102153984B1
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M11/00—Treating fibres, threads, yarns, fabrics or fibrous goods made from such materials, with inorganic substances or complexes thereof; Such treatment combined with mechanical treatment, e.g. mercerising
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K13/00—Devices for grooming or caring of animals, e.g. curry-combs; Fetlock rings; Tail-holders; Devices for preventing crib-biting; Washing devices; Protection against weather conditions or insects
- A01K13/006—Protective coverings
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- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M15/00—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment
- D06M15/19—Treating fibres, threads, yarns, fabrics, or fibrous goods made from such materials, with macromolecular compounds; Such treatment combined with mechanical treatment with synthetic macromolecular compounds
- D06M15/37—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
- D06M15/564—Polyureas, polyurethanes or other polymers having ureide or urethane links; Precondensation products forming them
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- D—TEXTILES; PAPER
- D10—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
- D10B—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
- D10B2401/00—Physical properties
- D10B2401/13—Physical properties anti-allergenic or anti-bacterial
Abstract
Description
본 발명은 애완동물 의류용 섬유에 관한 것으로, 더욱 상세하게는 다수의 미네랄을 혼합하여 구성한 복합체를 사용한 애완동물 의류용 섬유를 개시한다.The present invention relates to a fiber for pet clothing, and more particularly, discloses a fiber for pet clothing using a composite formed by mixing a plurality of minerals.
소득의 증가와 핵가족화에 따라 애완동물을 키우는 가정이 급속도로 늘고 있다. 이에 따라, 동물병원을 비롯한 애완동물의 음식, 미용, 의류 등을 취급하는 애완동물 시장이 활성화되고 있다. 특히 애완동물을 키우는 여성인구가 늘어나면서 애완동물의 외모를 예쁘게 꾸미기 위한 미용이나 의류 쪽 분야의 성장이 두드러진다. 애완동물에게 입히는 애완동물의 옷은 애완동물을 추위로부터 보호할 뿐만 아니라, 애완동물의 외모를 치장하기 위한 역할을 하는 것이다. The number of households with pets is increasing rapidly due to the increase in income and the becoming a nuclear family. Accordingly, a pet market that handles food, beauty, clothing, etc. of pets including veterinary hospitals is being activated. In particular, as the number of women who have pets increases, the growth in the fields of beauty and apparel to decorate the appearance of pets is remarkable. Pet clothes worn on pets not only protect the pet from the cold, but also serve to decorate the pet's appearance.
그러나 지금까지 이러한 애완동물용 의류에 있어서 건강기능을 제공하는 섬유는 시장에 나오지 않은 상태이다.However, until now, fibers that provide health functions in these pet clothing have not been on the market.
본 발명은 상기한 바와 같이 애완동물의 신체를 보호할 뿐만 아니라 건강기능을 제공할 수 있는 의류용 섬유를 제공하는데 목적이 있다.An object of the present invention is to provide a textile for clothing that can provide health functions as well as protecting the body of a pet as described above.
상기한 목적을 이루기 위한 수단으로서, 본 발명은 파이로필라이트, 세리사이트, 제올라이트, 및 몬모릴로나이트로 이루어진 혼합물로 상기 혼합물은 천연 미네랄 복합체를 포함하고 있으며 이러한 복합체는 각 구성성분을 나노입자화하여 분산 처리후 물리적 힘을 가하여 결합 결정구조를 변형시킨 복합체를 섬유에 적용시켜 이를 이용하여 애완동물용 의류를 제조한다.As a means to achieve the above object, the present invention is a mixture consisting of pyrophyllite, sericite, zeolite, and montmorillonite. The mixture contains a natural mineral complex, and such a complex is dispersed by nanoparticles of each component. After the treatment, a composite obtained by modifying the bonded crystal structure by applying a physical force is applied to the fiber, and the pet clothing is manufactured using this.
상기한 복합체를 적용한 섬유의 임상시험결과 통증완화, 지구력 증강, 운동력 향상, 항비만, 세포활성화 및 항균 탈취 효과를 제공하는 것으로 나타났다.As a result of the clinical test of the fiber to which the above complex was applied, it was found that it provides pain relief, endurance enhancement, exercise performance, anti-obesity, cell activation, and antibacterial deodorant effects.
이하 실시예를 통해 본 발명을 상세히 설명하기로 한다.The present invention will be described in detail through the following examples.
본 발명에 따른 천연 미네랄 복합체는 파이로필라이트, 세리사이트, 제올라이트, 및 몬모릴로나이트를 나노 입자 크기로 분쇄한 후에 이들 성분을 고르게 혼합하고 압착시켜서 복합체를 형성한다.The natural mineral composite according to the present invention forms a composite by pulverizing pyrophyllite, sericite, zeolite, and montmorillonite into nanoparticle sizes, and then evenly mixing and pressing these components.
상기 형성한 미네랄 복합체를 섬유에 코팅 결합하고, 이렇게 복합체가 코팅된 섬유를 사용하여 애완동물용 의류를 제조한다. 상기 코팅방법은 방수 및 투습성이 우수한 수성 우레탄 코팅법이 바람직하다.The formed mineral composite is coated on the fiber, and the composite-coated fiber is used to manufacture a pet clothing. The coating method is preferably a water-based urethane coating method excellent in waterproof and moisture permeability.
<실시예 1><Example 1>
상기 본 발명에 따른 애완동물용 의류를 랫드와 마우스를 이용한 동물모델을 대상으로 항비만, 항동증 및 지구력 향상에 미치는 효과를 살펴보았다.The effect of the pet clothing according to the present invention on anti-obesity, antihyperactivity and endurance improvement in an animal model using rats and mice was examined.
항비만 측정:Anti-obesity measurement:
- Sprague-Dawley 계 체중 200 g 전후의 웅성 랫드-Sprague-Dawley male rats weighing around 200 g
- 고지혈증에 의한 비만의 유발-Induction of obesity due to hyperlipidemia
- 체중, 장기 무게 측정-Weight, organ weight measurement
- 혈청 지질 성분의 측정-Measurement of serum lipid components
항통증 측정:Anti-pain measurement:
- 생후 6주령된 체중 28 g 정도의 웅성 ICR 마우스-Male ICR mice with a body weight of about 28 g at 6 weeks of age
- 급성통증모텔-Acute Pain Motel
- 초산 writhing 법-Acetic acid writhing method
- Hot Plate 법-Hot plate method
지구력 측정:Endurance measurement:
- 생후 6주령된 체중 28 g 정도의 웅성 ICR 마우스-Male ICR mice with a body weight of about 28 g at 6 weeks of age
- Swimming endurance capacity 측정-Swimming endurance capacity measurement
면역세포 측정:Immune Cell Measurement:
- RAW 264.7 대식세포에서의 세포형태 변화-Cell morphology change in RAW 264.7 macrophages
- RAW 264.7 대식세포에서의 세포증식능 측정-Measurement of cell proliferation ability in RAW 264.7 macrophages
- iNOS 유전자 발현 및 단백질 발현-iNOS gene expression and protein expression
1. 비만 실험1. Obesity experiment
시험 동물은 생후 6주령된 체중 28g 정도의 웅성 ICR 마우스와 Sprague-Dawley 계 체중 200g 전후의 웅성 랫트를 (주)대한 바이오링크로부터 구입하여 (재)경북테크노파크, 대구한의대학교, 효능검증원 동물사육실에서 일정한 조건 (온도: 22±2℃, 습도: 55±5%, 명암: 12시간 light/dark cycle)으로 1주일간 적응시킨 후 실험에 사용하였다.Test animals were 6 weeks old male ICR mice weighing 28 g and Sprague-Dawley male rats weighing 200 g from Daehan Biolink Co., Ltd. and purchased from Daehan Biolink (Re)Gyeongbuk Techno Park, Daegu Haany University, Efficacy Verification Institute. It was used in the experiment after acclimating for 1 week under certain conditions (temperature: 22±2℃, humidity: 55±5%, light/dark cycle: 12 hours light/dark cycle) in an animal breeding room.
고지혈증 유발에 의한 비만 유발은 노 등의 방법에 준하여 조제하여 사용한다. 고지혈유발식이 조성은 일반사료 100g 중에 cholesterol 1g, cholic acid 0.25 g, olive oil 2.5 g이 함유되도록 조제한다. 랫트의 한 군을 7마리로 하여 고지혈 유발 사료를 자유롭게 섭취토록 하면서 사육하였다. 고지혈 유발 식이를 2주간 섭취시킨 후 난괴법으로 사육상자에 한 마리씩 넣어 일반 식이로 교체 후 본 발명에 따른 섬유와 일반섬유를 깔짚 형태로 자른 후 섬유가 랫드의 몸에 자유스럽게 접촉하게 한 후 4 - 6주간 실험 사육하였다. Obesity induction caused by hyperlipidemia is prepared and used according to the method of Noh. The hyperlipidemia inducing diet composition is prepared so that 1 g of cholesterol, 0.25 g of cholic acid and 2.5 g of olive oil are contained in 100 g of general feed. One group of rats was 7 rats, and they were reared while freely ingesting the high-lipid-causing feed. After ingesting a diet that induces hyperlipidemia for 2 weeks, put one animal at a time in a breeding box by the egg mass method and replace it with a regular diet. The fibers and ordinary fibers were cut into a litter shape, and the fibers were allowed to freely contact the body of the rat, followed by experimental breeding for 4-6 weeks.
본 발명에 따른 섬유와 일반섬유의 접촉으로 의한 랫드 체중 변화와 상대적 장기 중량의 백분율을 구하기 위해 본 발명에 따른 섬유와 일반섬유의 접촉 시작 일로부터 종료 후에 각각의 랫드의 체중을 측정하였다. 체중을 측정한 후 랫드를 에테르로 마취시켜 21gage의 일회용 주사기를 사용하여 채혈하였다. 혈액을 2시간 정도 냉장상태에서 방치시킨 후 3,000rpm에 15분간 원심분리하여 혈청을 분리하였다. 간은 생리식염수로 문맥을 관류, 탈혈한 다음 여과지로 물기를 제거하여 무게를 측정하고, 복부지방, 비장, 고환, 및 신장의 무게도 생리식염수에 한번 씻은 후 물기를 제거하여 무게를 측정하였다.According to the invention According to the present invention, in order to obtain the percentage of the rat weight change and relative organ weight due to the contact between the fiber and the general fiber The weight of each rat was measured after the start of contact between the fiber and the normal fiber. After weighing, the rat was anesthetized with ether and blood was collected using a disposable syringe of 21 gage. The blood was left in a refrigerated state for about 2 hours and then centrifuged at 3,000 rpm for 15 minutes to separate the serum. The liver was perfused with physiological saline, the portal vein was debleed, and then the water was removed with a filter paper to measure the weight. Abdominal fat, spleen, testes, and kidneys were also washed once with physiological saline and then dried to measure the weight.
랫드에서 채혈한 혈액을 3,000rpm에서 15분간 원심분리하여 혈청을 분리하였다. 이 혈청을 아산제약 주식회사 혈청지질 함량 측정용 kit를 사용하여 total lipid (TL), total cholesterl (TC) 및 high density cholesterol (HDL)을 측정하였다. Low density cholesterol (LDL) 값은 다음과 같은 계산식을 이용하여 산출하였다. LDL cholesterol=Total cholesterol-(HDL cholesterol +triglyceride/5) Total lipid의 측정은 total lipid 측정용 kit (Advanced diagnostic Inc., USA)를 사용하여 측정하였다. The blood collected from rats was centrifuged at 3,000 rpm for 15 minutes to separate the serum. The serum was measured for total lipid (TL), total cholesterl (TC) and high density cholesterol (HDL) using a kit for measuring serum lipid content of Asan Pharmaceutical Co., Ltd. Low density cholesterol (LDL) value was calculated using the following calculation formula. LDL cholesterol = Total cholesterol-(HDL cholesterol + triglyceride/5) Total lipid was measured using a kit for measuring total lipid (Advanced diagnostic Inc., USA).
초산 writhing법:Acetic acid writhing method:
본 발명에 따른 섬유와 일반섬유에서 사육된 마우스에 각각 0.7 % 초산 (0.1 cc/10 g)을 복강에 주사하였다. 초산 투여 10분 후부터 10분간의 총writhing 횟수를 측정하였다.Each of the mice raised from fibers and ordinary fibers according to the present invention was injected with 0.7% acetic acid (0.1 cc/10 g) intraperitoneally. The total number of writhings for 10 minutes was measured from 10 minutes after acetic acid administration.
Hot Plate 법:Hot Plate Method:
본 발명에 따른 섬유와 일반섬유에서 사육된 마우스를 온도 55의 hot plate에 올려놓았을 때 뛰어 오를 때까지의 시간 (latency time)을 측정하였다. 조직 손상을 피하기 위해 cut off는 60초로 하였다.When the mice raised from the fibers and ordinary fibers according to the present invention were placed on a hot plate at a temperature of 55, the time until they jumped (latency time) was measured. Cut off was set to 60 seconds to avoid tissue damage.
3. 항피로 실험:3. Anti-fatigue test:
본 발명에 따른 섬유와 일반 섬유에서 사육된 마우스의 수영 지구력 (swimming endurance capacity) 측정을 통한 항피로 효과 (anti-fatigue capacity)를 측정하기 위해 아크릴 플라스틱 (20×20×45 cm)으로 제작한 마우스용 지구력 측정 장치를 이용하였다. 수온 25±0.1℃의 물을 수심 35 cm되도록 채우고 0.01 %의 계면활성제를 첨가한 후 수영 시간을 측정하였다. 마우스의 수영기록은 수영을 시작한 시점에서 사망시간까지를 측정하였다.According to the invention An endurance measuring device for mice made of acrylic plastic (20×20×45 cm) to measure the anti-fatigue capacity by measuring the swimming endurance capacity of mice raised from fibers and ordinary fibers. Was used. Water at a water temperature of 25±0.1° C. was filled to a depth of 35 cm, and 0.01% of a surfactant was added, and the swimming time was measured. The swimming record of the mouse was measured from the start of swimming to the time of death.
4. 면역세포에 미치는 영향 측정:4. Measurement of effects on immune cells:
세포주 배양Cell line culture
마우스 대식세포 세포주인 RAW 264.7 세포주를 ATCC로부터 분양 받아 2 mM glutamine이 함유된 DMEM 배지에 5% FBS, 100 U/ penicillin-streptomycin를 첨가한 배지에서 배양하여 실험에 사용하였다.The RAW 264.7 cell line, a mouse macrophage cell line, was pre-sold from ATCC and cultured in a medium containing 5% FBS and 100 U/penicillin-streptomycin in a DMEM medium containing 2 mM glutamine, and used in the experiment.
세포독성 효과Cytotoxic effect
RAW 264.7 대식세포에 대한 세포독성 효과를 살펴보기 위하여 Promega사의 CellTiter 96 AQueous One Solution Cell Proliferation Assay kit를 사용하여 이들 대식세포의 증식도를 조사하였다. RAW 264.7 세포를 1×104/well 세포가 되게 세포수를 조정하여 flat bottomed 96 well culture plate의 각 well에 분주한 일반섬유와 본 발명에 따른 섬유 위에서 배양하였다. 24시간 배양 후 배양액에 시약을 20 첨가한 후 37, 5 % CO2 배양기에 넣어 1시간 30분 배양한 후 ELISA reader 490 nm에서 측정하였다. 증식 결과는 실험군의 평균 O.D.에서 background O.D.를 뺀 값으로 표시하였다.In order to examine the cytotoxic effect on RAW 264.7 macrophages, Promega's CellTiter 96 AQ ueous One Solution Cell Proliferation Assay kit was used to investigate the proliferation of these macrophages. Normal fibers and fibers according to the present invention dispensed into each well of a flat bottomed 96 well culture plate by adjusting the number of RAW 264.7 cells to become 1×10 4 /well cells Incubated in the stomach. After culturing for 24 hours, 20 reagents were added to the culture solution, and then put into a 37, 5% CO 2 incubator and incubated for 1 hour and 30 minutes, followed by measurement with an ELISA reader at 490 nm. The proliferation result was expressed as the average OD of the experimental group minus the background OD.
항산화 효과Antioxidative effect
(가) iNOS유전자 발현( RT-PCR : 역전사 중합효소 연쇄반응)(A) iNOS gene expression (RT-PCR: reverse transcription polymerase chain reaction)
RNA 분리는 TRIzol를 이용하여 분리하였다. 간략하게 설명하면, RAW 264.7 대식세포에서 RNA를 분리하기 위하여 0.1% DEPC가 첨가된 PBS로 RAW 264.7 대식세포를 3회 세척 후 TRIzol 900 를 첨가하여 균질화 시켰다. 여기에 클로로포름 100 를 넣고 15분간 얼음에 정치 시켰다. 그 후 4, 12,000rpm에서 15분간 원심 분리하여 위층을 조심스럽게 취한 후 동량의 isopropanol을 첨가하여 -20에서 45분 정치한 후 원침하고, 70% DEPC-에탄올로 1회 세척하였다. RNA를 실온에서 건조시킨 후 DEPC가 첨가된 증류수에 일정량 희석하여 spectrophotometer에서 농도를 결정하였다. 5RT buffer 2 , 10 mM dATP 0.25 , 10 mM dGTP 0.25 , 10 mM dTTP 0.25 , 10 mM dCTP 0.25 , MMLV reverse transcriptase (200 U/) 0.25 , RNase inhibitor (28 U/) 0.25 , 50 oligo dT primer 0.5 , DEPC-DW 4 를 PCR tube에 넣어 RT-mixture를 만들고 여기에 total RNA를 첨가하였다. 이 시험관을 PCR machine (PTC-100TM Programmable Thermal Controllar; MJResearch, Inc.)에 넣어 42에서 60분간 열처리하여 역전사 반응을 완료하였다. PCR은 먼저 10 PCR buffer 3 , 25 mM MgCl2 1.8 , 10 mM dATP 0.3 , 10 mM dGTP 0.3 , 10 mM dTTP 0.3 , 10 mM dCTP 0.3 , 50 μM sense 및 antisense primer 0.25 , Taq polymerase (5 U/, Promega Co.) 0.25 를 혼합하고, 여기에 D.W.를 넣어 최종 용액량이 20 되게 하여 PCR mixture를 만들었다. PCR mixture를 PCR tube에 넣고 여기에 역전사 반응물 5 를 넣고 혼합한 뒤 PCR machine에 넣어 다음의 조건으로 PCR을 실시하였다. PCR 반응은 94에 3분간 1 cycle 반응 후, 94 45초, 57 45초, 72 45초간 35 cycles 반응시켰으며, 72에서 10분간 extension을 시행한 후 반응을 완료시켰다. 증폭된 산물은 1.2% agarose gel에 전기 영동 하여 UV transilluminater를 이용하여 DNA band를 확인하였다. RT-PCR에 사용한 primer는 (주)바이오니아사 (Bioneer Co. Choongbook)에 의뢰하여 합성하였으며, 각 primer의 염기서열은 Table 1과 같다.RNA was isolated using TRIzol. Briefly, to isolate RNA from RAW 264.7 macrophages, RAW 264.7 macrophages were washed three times with PBS to which 0.1% DEPC was added, and then TRIzol 900 was added to homogenize. Then, 100 chloroform was added and allowed to stand on ice for 15 minutes. Then, after centrifugation at 4, 12,000 rpm for 15 minutes, the upper layer was carefully taken, and the same amount of isopropanol was added, allowed to stand at -20 for 45 minutes, followed by centrifugation, and washed once with 70% DEPC-ethanol. After drying the RNA at room temperature, it was diluted in a certain amount in distilled water to which DEPC was added, and the concentration was determined on a spectrophotometer. 5RT buffer 2, 10 mM dATP 0.25, 10 mM dGTP 0.25, 10 mM dTTP 0.25, 10 mM dCTP 0.25, MMLV reverse transcriptase (200 U/) 0.25, RNase inhibitor (28 U/) 0.25, 50 oligo dT primer 0.5, DEPC -DW 4 was put into a PCR tube to make RT-mixture, and total RNA was added thereto. This test tube was put into a PCR machine (PTC-100 TM Programmable Thermal Controllar; MJResearch, Inc.) and heat-treated at 42 for 60 minutes to complete the reverse transcription reaction. PCR is first 10 PCR buffer 3, 25 mM MgCl 2 1.8, 10 mM dATP 0.3, 10 mM dGTP 0.3, 10 mM dTTP 0.3, 10 mM dCTP 0.3, 50 μM sense and antisense primer 0.25, Taq polymerase (5 U/, Promega Co.) 0.25 was mixed, and DW was added thereto to make a final solution amount of 20 to make a PCR mixture. The PCR mixture was put into a PCR tube, reverse transcription reaction product 5 was added, mixed, and then put into a PCR machine to perform PCR under the following conditions. The PCR reaction was carried out for 1 cycle for 3 minutes at 94, followed by 35 cycles for 45 seconds at 94, 45 seconds at 57, and 45 seconds at 72, and the reaction was completed after extension at 72 for 10 minutes. The amplified product was electrophoresed on a 1.2% agarose gel, and the DNA band was confirmed using a UV transilluminater. The primers used for RT-PCR were synthesized by requesting Bioneer Co. Choongbook, and the base sequence of each primer is shown in Table 1.
(나) iNOS 단백질 발현 (Western blotting)(B) iNOS protein expression (Western blotting)
세포에서의 단백질 분리는 lysis buffer (50mM Tris-Cl, 25mM EDTA, 650mM NaCl, 5% Triton X-100, 100X PMSF, 100X protease inhibitor coctail, 5X lysis buffer)를 이용하여 분리하였다. 추출된 단백질을 95에서 5분간 가열한 후 12% SDS-polyacrlyamide gel에 전기영동 후 Protran nitrocellulose membrane에 전이시켰다. 10% skim milk를 함유한 TPBS(0.1% Tween 20 in PBS)를 이용하여 incubation하면서 비특이적인 단백질에 대한 blocking을 실시하고, anti-iNOS 항체와 함께 incubation 후 ECL 용액을 적용시킨 다음 X-ray film에 감광시켜 iNOS 단백질 발현을 조사하였다. 대조 단백질 밴드로 HSP70을 사용하여 상기와 동일한 방법으로 관찰하였다.Protein separation from cells was isolated using lysis buffer (50mM Tris-Cl, 25mM EDTA, 650mM NaCl, 5% Triton X-100, 100X PMSF, 100X protease inhibitor coctail, 5X lysis buffer). After heating the extracted protein at 95 for 5 minutes, electrophoresis on 12% SDS-polyacrlyamide gel and Protran It was transferred to the nitrocellulose membrane. Blocking for non-specific proteins was performed while incubating with TPBS (0.1% Tween 20 in PBS) containing 10% skim milk, incubated with anti-iNOS antibody, and then ECL solution was applied to the X-ray film. The iNOS protein expression was examined by photosensitization. HSP70 was used as a control protein band and observed in the same manner as above.
5. 통계학적 분석5. Statistical analysis
모든 실험결과는 mean±S.E로 나타내었고, 각 그룹간의 측정치에 대한 자료분석은 ANOVA test를 이용하여 p<0.05 수준에서 유의성을 판정하였다.All experimental results were expressed as mean±S.E, and significance was determined at p<0.05 level using the ANOVA test for data analysis on the measured values between each group.
최종 실험결과Final experiment result
1. One. 랫드에On the rat 대한 고지혈증 유발효과 Hyperlipidemia inducing effect
노 등의 방법에 의한 고지혈 유발 식이를 2 주간 섭취한 군에서 혈청중의 Total lipid (TL), Total cholesterol (TC), High density lipoprotein-cholesterol (HDL) 및 low density lipoprotein-cholesterol (LDL) 농도의 변화를 살펴보기 위해 혈청을 분리하여 혈청지질함량 측정용 kit를 이용하여 측정한 결과는 Table 2 에서 볼 수 있듯이 정상 식이를 투여한 군에 비해 1 % 콜레스테롤이 첨가된 식이에서 TL, TC, LDL에서 유의하게 증가됨이 관찰되었으며, HDL의 경우 1 % 콜레스테롤이 첨가된 식이에서 정상식이군에 비해 유의하게 감소됨이 관찰되었다. 실험군을 나눌 때 각 실험군간의 유의성이 관찰되지 않게 실험군을 조정하였다. The concentration of total lipid (TL), total cholesterol (TC), high density lipoprotein-cholesterol (HDL) and low density lipoprotein-cholesterol (LDL) in serum in the group who ingested a high-lipidemia-inducing diet by No et al. for 2 weeks. To examine the changes, serum was separated and measured using a serum lipid content measurement kit, as shown in Table 2, in TL, TC, and LDL in a diet containing 1% cholesterol compared to the group administered with a normal diet. It was observed that a significant increase was observed, and in the case of HDL, it was observed that the 1% cholesterol was significantly decreased compared to the normal diet group. When dividing the experimental groups, the experimental groups were adjusted so that no significance between each experimental group was observed.
Values are means±S.E. of 6 or 7 rats.Values are means±S.E. of 6 or 7 rats.
TL : total lipidTL: total lipid
TC : total cholesterol TC: total cholesterol
HDL : high density lipoprotein-cholesterolHDL: high density lipoprotein-cholesterol
LDL : low density lipoprotein-cholesterolLDL: low density lipoprotein-cholesterol
Different letter indicates significant difference between groups by p<0.05.Different letter indicates significant difference between groups by p<0.05.
2. 2. 랫드의Rat 체중증가 Weight gain
정상식이군에서 본 발명에 따른 섬유와 일반섬유, 고지혈식이군에서 본 발명에 따른 섬유 섬유와 일반섬유에 속한 랫트들의 실험 기간 동안의 체중 변화는 Table 3, 4 과 같다.Fibers and general fibers according to the present invention in the normal diet group, fibers according to the invention in the hyperlipidemia diet group Tables 3 and 4 show the weight change during the experiment of the rats belonging to fiber and general fiber.
일반섬유와 본 발명에 따른 섬유 섬유를 깔짚으로 하여 정상식이를 자유로이 섭취하도록 하여 랫드를 사육시킨 후 체중의 증가량을 관찰한 결과, 일반섬유의 경우, 4.09±14g이 증가한 반면, 본 발명에 따른 섬유에서 사육된 랫드는 3.62±12 g이 증가됨이 관찰되었다. 본 발명에 따른 섬유에서 사육된 랫드는 평균적으로 12.9 %정도 일반섬유에서 사육된 랫드보다 체중 증가가 억제됨이 관찰되었다. 이는 체중증가의 억제 요인이 본 발명에 따른 섬유 섬유의 어떤 성분에 기인한 것이라 생각될 수 있으나 여기에 관한 세부적인 연구가 이루어져야 할 것이다.General fibers and fibers according to the invention As a result of observing the increase in body weight after breeding rats with fiber as a litter to freely consume a normal diet, in the case of general fiber, 4.09±14g increased, whereas according to the present invention It was observed that the rats raised in fiber increased 3.62±12 g. It was observed that the rats bred from fibers according to the present invention had an average of 12.9% suppressed weight gain compared to the rats bred from ordinary fibers. This is the fiber according to the present invention as an inhibitory factor for weight gain. It can be thought that it is due to a certain component of the fiber, but detailed research on this should be done.
Values are means?S.E. of 6 rats.Values are means?S.E. of 6 rats.
Average weight gain during 6 weeks experiment.Average weight gain during 6 weeks experiment.
Initial means body weight of rats at starting point of experiment.Initial means body weight of rats at starting point of experiment.
N : general textile + normal diet (n=6)N: general textile + normal diet (n=6)
NS : Solatontex + normal diet (n=6)NS: Solatontex + normal diet (n=6)
Different letter indicates significant difference between groups by p<0.05.Different letter indicates significant difference between groups by p<0.05.
Values are means±S.E. of 7 rats.Values are means±S.E. of 7 rats.
Average weight gain during 4 weeks experiment.Average weight gain during 4 weeks experiment.
Initial means body weight of rats at starting point of experiment.Initial means body weight of rats at starting point of experiment.
H : hypercholesterolemic rats + general textile + normal diet (n=7)H: hypercholesterolemic rats + general textile + normal diet (n=7)
HS : hypercholesterolemic rats + Solatontex + normal diet (n=7)HS: hypercholesterolemic rats + Solatontex + normal diet (n=7)
3. 3. 랫드의Rat 장기중량 Long-term weight
본 발명에 따른 섬유와 일반 섬유에서 사육 시 정상식이군과 고지혈식이군에서 복부지방, 간장, 신장, 비장 및 고환의 무게에 미치는 영향을 살펴본 결과는 Table. 5 ,6과 같다. 복부지방의 경우 정상식이와 고지혈식이 후 정상식이로 교체한 군 모두에서 본 발명에 따른 섬유 섬유를 깔짚으로 하여 사육한 랫드에서 일반 섬유에서 사육된 랫드에서보다 복부지방의 무게가 적게 관찰되었다. 간의 경우에서도 본 발명에 따른 섬유 섬유를 깔짚으로 하여 사육한 랫드에서 일반 섬유에서 사육된 랫드에서보다 간의 무게가 적게 관찰되었다. 그 외 신장, 비장 및 고환에서는 별 차이를 관찰 할 수 없었다.According to the invention Table. Same as 5 and 6. In the case of abdominal fat, fibers according to the present invention in both the normal diet and the group replaced with a normal diet after a hyperlipidemia diet The weight of abdominal fat was observed in rats reared with fiber litter compared to rats reared with regular fibers. In the case of liver, it was observed that the weight of the liver was lower in the rats raised with the fibrous fibers according to the present invention as compared to the rats raised with the general fibers. Other differences were not observed in the kidney, spleen and testis.
4. 4. 총혈청Total serum 지방량의 변화에 미치는 영향 Effects on changes in fat mass
본 실험에서는 일반섬유와 본 발명에 따른 섬유를 깔짚으로 만들어 정상식이와 고지혈 식이 후 총지방량의 변화를 조사하였다. 정상식이를 6주간 하면서 총혈청 지방량의 증가량을 측정한 결과 일반섬유에서 사육한 경우 처음보다 27.2 %가 증가한 반면, 본 발명에 따른 섬유에서는 8.7 %가 증가됨이 관찰되었다. 본 발명에 따른 섬유에서 사육된 랫드는 평균적으로 18.5 % 정도 일반섬유에서 사육된 랫드보다 총혈청 지방량의 증가량이 억제됨이 관찰되었다.In this experiment, general fibers and fibers according to the present invention were made into litter to investigate the change in total fat mass after a normal diet and a high-lipid diet. As a result of measuring the increase in total serum fat mass while following a normal diet for 6 weeks, it was observed that the increase in total serum fat increased by 27.2% compared to the first in the case of breeding in normal fiber, whereas the increase in 8.7% was observed in the fiber according to the present invention. It was observed that the increase in total serum fat mass was suppressed in the rats raised from fibers according to the present invention, on average, about 18.5% compared to the rats raised from ordinary fibers.
고지혈식이 후 정상식이로 교체하여 일반 섬유와 본 발명에 따른 섬유 섬유에서 4주간 사육 후 총혈청 지방량의 감소률을 측정한 결과, 일반 섬유의 경우 2.9 % 감소하였으나, 본 발명에 따른 섬유의 경우 7.7 %의 감소가 관찰되었다.As a result of measuring the reduction rate of total serum fat mass after 4 weeks of breeding in general fibers and fibrous fibers according to the present invention by replacing the hyperlipidemia diet with a normal diet, in the case of normal fibers, it decreased by 2.9%, but in the case of the fibers according to the present invention, 7.7 % Reduction was observed.
5. 혈청 5. Serum TCTC , HDL 및 LDL의 변화에 미치는 영향, HDL and LDL changes
본 실험에서는 일반섬유와 본 발명에 따른 섬유를 깔짚으로 만들어 정상식이와 고지혈 식이 후 혈청 TC, HDL 및 LDL의 변화를 조사하였다. 정상식이를 6주간 하면서 TC, HDL 및 LDL 변화량을 측정한 결과 일반섬유에서 사육한 경우 TC의 양은 98.3±4.9 mg/dl 이었으며, 본 발명에 따른 섬유에서는 사육한 경우 107.3±4.6 mg/dl가 증가됨이 관찰되었다. HDL의 경우 일반섬유에서 사육한 경우 9.8±2.4 mg/dl 이었으며, 본 발명에 따른 섬유에서는 사육한 경우 12.5±1.9 mg/dl로 관찰되었다. LDL의 경우 일반섬유에서 사육한 경우 21.0±4.7 mg/dl 이었으며, 본 발명에 따른 섬유에서는 사육한 경우 25.2±3.6 mg/dl가 증가됨이 관찰되었다.In this experiment, the changes in serum TC, HDL, and LDL were investigated after a normal diet and a high-lipid diet by making the fiber according to the present invention as a litter. As a result of measuring the amount of change in TC, HDL and LDL during 6 weeks of normal diet, the amount of TC was 98.3±4.9 mg/dl when bred in normal fiber, and 107.3±4.6 mg/dl was increased when bred in fiber according to the present invention. Was observed. In the case of HDL, it was observed to be 9.8±2.4 mg/dl when bred in normal fiber, and 12.5±1.9 mg/dl when bred in the fiber according to the present invention. In the case of LDL, it was observed that 21.0±4.7 mg/dl was raised in normal fiber, and 25.2±3.6 mg/dl was increased when bred in the fiber according to the present invention.
고지혈식이 후 일반식이로 교체하여 일반 섬유와 본 발명에 따른 섬유 섬유에서 4주간 사육 후 TC, HDL 및 LDL의 변화를 측정한 결과, TC의 감소량은 34.14 %이었으며, 본 발명에 따른 섬유에서는 사육한 경우 39.2±3.4 %가 감소됨이 관찰되었다. HDL의 경우 일반섬유에서 사육한 경우 24.0±1.7 mg/dl 이었으며, 본 발명에 따른 섬유에서는 사육한 경우 23.9±1.4 mg/dl로 관찰되었다. LDL의 경우 일반섬유에서 사육한 경우 25.8±3.8 mg/dl 이었으며, 본 발명에 따른 섬유에서는 사육한 경우 29.2±2.4 mg/dl가 증가됨이 관찰되었다.As a result of measuring changes in TC, HDL, and LDL after 4 weeks of breeding in normal fibers and fiber fibers according to the present invention by replacing the diet with normal diet after hyperlipidemia, the decrease in TC was 34.14%, and the fiber according to the present invention was bred in the fiber according to the present invention. It was observed that a decrease of 39.2±3.4% was observed. In the case of HDL, it was 24.0±1.7 mg/dl when bred in normal fiber, and 23.9±1.4 mg/dl when bred in the fiber according to the present invention. In the case of LDL, it was observed that 25.8±3.8 mg/dl was raised in normal fiber, and 29.2±2.4 mg/dl was increased when bred in the fiber according to the present invention.
6. 급성 통증 완화 효과6. Acute pain relief effect
가) 초산 writhing 법A) acetic acid writhing method
본 발명에 따른 섬유 섬유에서 사육된 경우 초산 writhing 법에서는 평균 writhing 횟수가 10분간 25.8±4.6회였으며, 일반섬유의 경우 평균 writhing 횟수는 25.5±3.5회였다. 급성통증 모델 중의 하나인 초산 writhing 법에서 본 발명에 따른 섬유 섬유에서 사육된 마우스와 일반섬유에서 사육된 마우스에서 writhing 횟수에서의 차이는 관찰되지 않았다. Fiber according to the invention When bred in fibers, the average number of writhings was 25.8±4.6 times for 10 minutes in the acetic acid writhing method, and the average number of writhings was 25.5±3.5 times for ordinary fibers. In the acetic acid writhing method, which is one of the acute pain models, there was no difference in the number of writhings between the mice raised from the fibrous fibers according to the present invention and the mice raised from the general fibers.
나) Hot plate 법B) Hot plate method
본 발명에 따른 섬유에서 사육된 경우 Hot plate에서의 통증 완화 효과를 측정한 결과 본 발명에 따른 섬유 사육군의 평균 latency는 26.7±0.9초였으며, 일반섬유의 경우 평균 20.9 ±1.8초였다. 일반 섬유에 비해 약 27.7 %의 항통증효과가 증가됨이 관찰되었다. 급성통증 모델 중의 하나인 Hot plate 법에서 본 발명에 따른 섬유 섬유에서 사육된 마우스가 일반섬유에서 사육된 마우스에 비해 오랫동안 Hot plate에 견디는 것으로 보아 Hot plate에 의해 유도된 통증을 완화하는데 효과가 있음을 나타낸 결과로 생각된다.As a result of measuring the pain relief effect in the hot plate when bred from the fibers according to the present invention, the average latency of the fiber breeding group according to the present invention was 26.7±0.9 seconds, and the average of 20.9±1.8 seconds in the case of ordinary fibers. It was observed that the anti-pain effect was increased by about 27.7% compared to normal fiber. In the hot plate method, which is one of the acute pain models, mice raised on fibrous fibers according to the present invention endure a hot plate for a long time compared to mice raised on ordinary fibers, so it is effective in relieving pain induced by hot plates. It is believed to be the result shown.
7. 수영 지구력 측정7. Swimming endurance measurement
수영 지구력 측정 결과, 본 발명에 따른 섬유 섬유에서 사육된 마우스와 일반섬유에서 사육된 마우스의 swiming endurence capacity를 비교해 볼 때 일반섬유의 경우 3.46±0.4 초이었으나, 본 발명에 따른 섬유 섬유에서 사육된 경우 4.25±0.5 초로 관찰되어, 본 발명에 따른 섬유는 일반 섬유에 비해 약 22.8 % 지구력이 증가됨이 관찰되었다. 이와 같이 수영 시간이 본 발명에 따른 섬유 섬유에서 사육된 마우스에서 증가된 것은 본 본 발명에 따른 섬유 섬유가 항피로 효과를 발휘하여 실험동물의 수영 지구력을 증가시켜 나타난 결과로 생각된다.Swimming endurance measurement results, fibers according to the invention When comparing the swiming endurence capacity of mice raised on fiber and mice raised on normal fiber, it was 3.46±0.4 seconds for normal fiber, but the fiber according to the present invention When bred in the fiber was observed at 4.25 ± 0.5 seconds, the fiber according to the present invention was observed to increase the endurance by about 22.8% compared to the normal fiber. In this way, the increase in swimming time in the mice raised in the fiber fiber according to the present invention is the fiber according to the present invention. Fiber exerts anti-fatigue effect, which is believed to be the result of increasing swimming endurance of experimental animals.
8. 면역세포에 미치는 영향 측정8. Measurement of effects on immune cells
가) 대식세포의 형태 변화에 미치는 영향A) Effect on the change in the morphology of macrophages
면역세포인 대식세포에 미치는 효과를 측정하기 위해 배양접시에 배양된 대식세포를 일반섬유와 기능성 섬유를 크기가 큰 배양접시에 깔고 그 위에 다시 배양된 배양접시를 올려놓은 후 파동에 의한 다른 세포에 미치는 영향을 배제하기 위하여 알루미늄 호일로 분리시킨 후 24시간, 48시간 관찰하면서 세포의 형태 변화와 세포수에 미치는 영향을 관찰하였다. 본 발명에 따른 섬유 섬유, 일반섬유, 일반배양의 경우 24시간 48시간 모두에서 세포형태의 변화와 세포수의 차이는 관찰 할 수 없었다.In order to measure the effect on macrophages, which are immune cells, the macrophages cultured in a culture dish are spread on a large culture dish with normal fibers and functional fibers, and the culture dish is placed on the culture dish again. In order to rule out the effect, the cells were separated with aluminum foil and observed for 24 hours and 48 hours to observe the change in cell morphology and the effect on the number of cells. Fiber according to the invention In the case of fiber, normal fiber, and normal culture, no change in cell morphology and difference in cell number could be observed at all 24 hours and 48 hours.
나) iNOS유전자 및 단백질 발현에 미치는 영향B) Effect on iNOS gene and protein expression
주로 대식세포에서 생성되는 nitric oxide (NO)는 작고 불안정한 무기가스로 여러 가지 생물학적 성질을 가지는 것으로 알려져 있다. NO는 사람을 포함한 고등동물 뿐만 아니라 하등동물에서도 합성되며 합성된 NO는 생체내의 신호전달 물질로 수 많은 생리적 과정에 관여하고 있음이 밝혀지고 있다. 즉 NO는 신경전달, 혈압의 조절 및 종양세포나 세포내 기생 생물에 대한 숙주 면역게의 방어기능등에 관여하는 것으로 알려져 있다. NO는 저농도에서는 신경전달 물질 등과 같은 작용을 하나, 과도한 NO 생성은 염증에 관여하여 숙주세포의 파괴와 염증조직의 상해를 초래하는 것으로 보고되고 있다.Nitric oxide (NO), mainly produced in macrophages, is a small, unstable inorganic gas and is known to have various biological properties. NO is synthesized not only in higher animals including humans, but also in lower animals, and it has been found that synthesized NO is a signaling material in vivo and is involved in numerous physiological processes. In other words, NO is known to be involved in neurotransmission, regulation of blood pressure, and defense functions of host immune crabs against tumor cells or intracellular parasites. NO acts like a neurotransmitter at low concentrations, but excessive NO generation is reported to be involved in inflammation, causing destruction of host cells and injury to inflammatory tissues.
내적, 외적으로 생성되는 각종 장애인자의 축적으로 인하여 여러 가지 질병이 유발되는데, 이중 free radical은 생체막을 공격하여 지질 radical 연쇄반응에 의하여 과산화지질을 축적 시켜 각종 순환장애, 동맥경화, 노화, 내분비 질환 등을 유발하는 것으로 알려져 있다. NO는 지난 수년간 가장 연구가 많이 된 free radical 중의 하나로 알려져 있다. 따라서 본 발명에 따른 섬유의 NO생성과 관련된 유전자 발현에 미치는 영향을 살펴보기 위해, 배양접시에 배양된 대식세포를 일반섬유와 기능성 섬유를 크기가 큰 배양접시에 깔고 그 위에 다시 배양된 배양접시를 올려놓은 후 파동에 의한 다른 세포에 미치는 영향을 배제하기 위하여 알루미늄 호일로 분리시킨 후 24시간 배양하였다. 배양된 RAW 264.7 세포주로부터 분리한 iNOS mRNA를 각각 PCR 반응시키고, 생성된 DNA를 전기 영동하여 비교하였을 때, 본 발명에 따른 섬유 섬유, 일반섬유 및 일반배양에서 모두 iNOS mRNA 유전자 발현을 관찰 할 수 없었다(Fig. 13). 또한 본 발명에 따른 섬유의 LPS에 의해 유도된 iNOS 단백질 발현에 미치는 영향을 살펴본 결과, LPS만 단독으로 처리한 RAW 264.7 세포주와 LPS 및 본 발명에 따른 섬유 및 일반섬유에서 배양된 RAW 264.7 세포주로부터 분리한 iNOS 단백질 발현의 차이는 관찰 할 수 없었다. 따라서, 본 실험 결과로만 판단할 경우, 본 발명에 따른 섬유의 기능 중 free radical중의 하나인 NO의 조절에는 효과가 없거나 미약하다고 사료된다.Various diseases are caused by the accumulation of people with disabilities that are generated internally and externally. Of these, free radicals attack biological membranes and accumulate lipid peroxidation through lipid radical chain reaction, resulting in various circulation disorders, arteriosclerosis, aging, endocrine diseases, etc. It is known to cause. NO is known as one of the most studied free radicals over the years. Therefore, in order to examine the effect on the gene expression related to NO production of fibers according to the present invention, macrophages cultured in a culture dish are laid on a large-sized culture dish, and a culture dish cultured again is placed on it. After placing, in order to exclude the influence on other cells by the wave, they were separated with aluminum foil and cultured for 24 hours. When iNOS mRNA isolated from the cultured RAW 264.7 cell line was subjected to PCR reaction, and the resulting DNA was compared by electrophoresis, the fiber according to the present invention The iNOS mRNA gene expression could not be observed in fiber, normal fiber and normal culture (Fig. 13). In addition, as a result of examining the effect of the fiber according to the present invention on the expression of iNOS protein induced by LPS, the RAW 264.7 cell line treated with only LPS and LPS and the fiber according to the present invention And the difference in the expression of the iNOS protein isolated from the RAW 264.7 cell line cultured in normal fiber could not be observed. Therefore, judging only from the results of this experiment, it is considered that the control of NO, one of the free radicals, among the functions of the fiber according to the present invention is ineffective or weak.
결과 요약Summary of results
기능성 섬유인 본 발명에 따른 섬유의 항비만, 항통증 및 지구력 향상효과 검사 결과 다음과 같은 항비만, 항통증 및 지구력 향상효과를 나타냄으로서 기능성 섬유로의 가능성이 제시되었다.As a result of testing the anti-obesity, anti-pain, and endurance enhancing effects of the fiber according to the present invention, which is a functional fiber, the possibility as a functional fiber was suggested by showing the following anti-obesity, anti-pain, and endurance enhancing effects.
1. 일반섬유와 본 발명에 따른 섬유 섬유를 깔짚으로 하여 정상식이를 자유로이 섭취하도록 하여 랫드를 사육시킨 후 체중의 증가량을 관찰한 결과, 일반섬유의 경우 4.09±0.14 g이 증가한 반면, 본 발명에 따른 섬유에서 사육된 랫드는 3.62±0.12 g의 증가가 관찰되었다. 본 발명에 따른 섬유에서 사육된 랫드는 평균적으로 12.9 %정도 일반섬유에서 사육된 랫드보다 체중 증가가 억제됨이 관찰되었다. 1. General fibers and fibers according to the present invention As a result of observing the increase in body weight after breeding rats with fiber as a litter to freely consume a normal diet, in the case of general fiber, 4.09±0.14 g increased, whereas in the case of rats bred from fiber according to the present invention, 3.62±0.12 An increase in g was observed. It was observed that the rats bred from fibers according to the present invention had an average of 12.9% suppressed weight gain compared to the rats bred from ordinary fibers.
2. 복부지방의 경우 정상식이군에서 일반섬유에서 사육한 경우 1.62±0.06 g이였으며, 본 발명에 따른 섬유 섬유에서 사육한 경우 1.52±0.08 g이었다. 고지혈식이 후 정상식이로 교체한 후 일반섬유에서 사육한 경우, 복부지방의 무게가 1.64±0.05 g이었으며, 본 발명에 따른 섬유 섬유를 깔짚으로 하여 사육한 랫드의 복부지방은 1.53±0.06 g 으로 일반섬유에서 사육된 랫드에서 보다 복부지방의 무게가 적게 관찰되었다.2. In the case of abdominal fat, 1.62±0.06 g was obtained when reared on normal fiber in the normal diet group, and the fiber according to the present invention It was 1.52±0.08 g when bred in fiber. When rearing from normal fiber after replacing with a normal diet after a hyperlipidemia diet, the weight of the abdominal fat was 1.64±0.05 g, and the fiber according to the present invention The abdominal fat of the rats reared with fiber litter was 1.53±0.06 g, and the weight of the abdominal fat was less than that of the rats reared with normal fiber.
3. 본 발명에 따른 섬유 섬유와 일반 섬유를 깔짚으로 하여 랫드를 정상식이를 하면서 6주간 사육한 후 총혈청 지방량의 증가량을 측정한 결과, 일반섬유에서 사육한 경우 처음보다 27.2 % 가 증가한 반면, 본 발명에 따른 섬유 섬유에서는 8.7 % 정도만 증가됨이 관찰되었다. 본 발명에 따른 섬유에서 사육된 랫드는 평균적으로 18.5 % 정도 일반섬유에서 사육된 랫드보다 총혈청 지방량의 증가량이 억제됨이 관찰되었다.3. Fiber according to the invention As a result of measuring the increase in total serum fat content after raising the rats for 6 weeks while using fiber and regular fiber as a litter, the fiber according to the present invention increased by 27.2% from the first when reared on regular fiber. It was observed that only an increase of 8.7% was observed in the fiber. It was observed that the increase in total serum fat mass was suppressed in the rats raised from fibers according to the present invention, on average, about 18.5% compared to the rats raised from ordinary fibers.
4. 고지혈식이 후 정상식이로 교체하여 일반섬유와 본 발명에 따른 섬유의 섬유에서 4주간 사육 후 총혈청 지방 감소량을 측정한 결과 일반 섬유의 경우 처음보다 2.9 % 감소하였으나, 본 발명에 따른 섬유 섬유의 경우 7.7 %의 감소가 관찰되었다.4. As a result of measuring total serum fat reduction after 4 weeks of breeding in normal fibers and fibers of the fiber according to the present invention by replacing the hyperlipidemia diet with a normal diet, in the case of normal fibers, the amount of total serum fat decreased by 2.9%, but the fiber fibers according to the present invention In the case of, a decrease of 7.7% was observed.
5. 급성통증 모델 중의 하나인 Hot plate법을 이용하여, 본 발명에 따른 섬유의 항 통증 효과를 살펴 본 결과, 본 발명에 따른 섬유 섬유 처리군의 평균 latency는 26.7±0.9초였으며, 일반섬유의 경우 평균 20.9±0.8초로 일반 섬유에 비해 약 27.7 %의 항통증효과가 관찰되었다.5. Using the hot plate method, one of the acute pain models, the fiber according to the present invention As a result of examining the anti-pain effect, the fiber according to the present invention The average latency of the fiber treatment group was 26.7±0.9 seconds, and the average latency of the normal fibers was 20.9±0.8 seconds.
6. 지구력 향상 효과를 살펴보기 위해 본 발명에 따른 섬유 섬유와 일반섬유에서 사육된 마우스의 swiming endurence capacity를 비교해 한 결과, 일반 섬유에서 사육된 마우스의 경우 3.46±0.4초의 수영 지속이 관찰되었으며, 본 발명에 따른 섬유 섬유의 경우 4.25±0.5초로 일반 섬유에 비해 약 22.8 % 지구력이 증가됨이 관찰되었다. 6. Fiber according to the present invention to examine the effect of improving endurance As a result of comparing the swiming endurence capacity of mice raised on fibers and ordinary fibers, a swimming duration of 3.46±0.4 seconds was observed in mice raised on ordinary fibers, and 4.25±0.5 seconds in the case of the fiber fibers according to the present invention. Compared to that, it was observed that the endurance was increased by about 22.8%.
Claims (4)
파이로필라이트, 세리사이트, 제올라이트 및 몬모릴로나이트의 혼합물을 나노 입자 크기로 분쇄하여, 고르게 혼합하고 압착시켜 복합체를 형성한 후, 상기 복합체를 섬유에 코팅시킨 애완동물 의류용 섬유.In the fiber for pet clothing using a mineral composite,
A mixture of pyrophilite, sericite, zeolite, and montmorillonite is pulverized into a nanoparticle size, mixed evenly and compressed to form a composite, and then the composite is coated on the fiber.
상기 복합체를 방수 및 투습성이 우수한 수성 우레탄을 이용하여 섬유에 코팅하는 것을 특징으로 하는 애완동물 의류용 섬유.The method of claim 1,
Fiber for pet clothing, characterized in that the composite is coated on the fiber using water-based urethane having excellent waterproof and moisture permeability.
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| KR100938914B1 (en) * | 2009-11-05 | 2010-01-27 | 강양식 | A functional fabric and a manufacture method thereof |
| KR101292882B1 (en) * | 2005-04-18 | 2013-08-02 | 군제 가부시키가이샤 | Fiber highly cool to touch |
| KR20180027934A (en) * | 2016-09-07 | 2018-03-15 | 한국과학기술연구원 | Fiber complexes and methods of manufacturing the same |
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| KR101292882B1 (en) * | 2005-04-18 | 2013-08-02 | 군제 가부시키가이샤 | Fiber highly cool to touch |
| KR100938914B1 (en) * | 2009-11-05 | 2010-01-27 | 강양식 | A functional fabric and a manufacture method thereof |
| KR20180027934A (en) * | 2016-09-07 | 2018-03-15 | 한국과학기술연구원 | Fiber complexes and methods of manufacturing the same |
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