KR102137901B1 - Natural ingredients Disinfection Materials Available in Multi-use Facilities - Google Patents

Abstract

The present invention relates to antibacterial, antifungal and pesticidal compositions against harmful microorganisms applied in multi-use facilities, wherein the compositions contain cinnamon, pulegone, geranial, cineol, menthol and Chamaecyparis obtusa as active ingredients. Specifically, the compositions of the present invention show a significant disinfecting effect against harmful microorganisms and harmful pests that are detected in the multi-use facilities and cause disease, and can be usefully used for disinfection for spaces in the multi-use facilities through a safe and eco-friendly method that is not harmful to the human body.

Description

Natural ingredients disinfection materials available in multi-use facilities

The present invention relates to a composition for disinfection derived from natural ingredients for indoor air quality management that can be used in facilities used by an unspecified number of people, that is, underground stations and underground shopping malls, terminals, museums, exhibition halls, libraries, general hospitals, and movie theaters.

According to a recent survey, about 87% of Koreans live in indoor spaces per day, and MERS-CoV, severe acute respiratory syndrome (SARS), swine flu, and birds From the problems of various air infections, such as the flu and foot and mouth disease, the interest in indoor infection and indoor air quality is increasing.

The modern society faces a new problem of environmental pollution as the population continues to grow rapidly with the increase of population, and the main causes of such environmental pollution are air pollution, water pollution, soil pollution, waste, noise, and vibration. Appears as

This increase in pollutants has brought about changes in the environment such as global warming, and modern people live in indoor spaces of multi-use facilities such as homes, offices, and sports facilities for more than 80% to 90% of the day. It can be said that the time of exposure to harmful substances is longer than the time of exposure to the atmosphere (see FIG. 1 ). As a result, interest in indoor air quality (IAQ) has increased recently, and research has been conducted by many researchers.

Among the indoor air pollutants, five substances such as fine dust (PM10), carbon dioxide (CO2), formaldehyde (HCHO), total bacterial bacteria, and carbon monoxide (CO) are set. In addition, voluntary compliance is established by setting recommended standards for five pollutants, such as nitrogen dioxide (NO2), radon (Rn), total volatile organic compounds (TVOC), asbestos, and ozone (O3), which have external pollutants or have relatively low pollution levels. Induction.

On the other hand, when the general public coughs once, the number of bioaerosols discharged varies depending on the researcher, but it is reported that the number of bioaerosols reaches from 50 to as many as 10,000, and the diameter of the bioaerosol reaches about 12 or 14 micrometers.

However, these studies are only for diameters of 1 micrometer or more, and bioaerosols of 1 micrometer or less are separated during conversation or daily breathing, and it is reported that there is a large variation among people. In particular, there are people who discharge large amounts of bioaerosol through coughing and conversation, and transmission through bioaerosols through people with these characteristics is greatly concerned as an important source of transmission in multi-use facilities. Recently, as the utilization of underground spaces, which are structurally difficult to manage indoor air quality, has increased in Korea, problems have arisen due to deterioration of indoor air quality, and research on indoor air quality began in the late 1980s.

Since the 2000s, with the terms building syndrome, sick house syndrome, etc., since the Indoor Air Quality Control Act was promulgated in 2003, various studies have been conducted on indoor air pollution from common facilities, schools, reading rooms, and unapplied facilities. It has been.

Indoor air quality pollution refers to the condition in which the indoor air in the above-mentioned multi-use facility is contaminated and can be caused by very complicated causes, but the effect is not long enough to threaten the lives of indoor residents, but in the long run, health It cannot be denied that it has an adverse effect, and such a problem of indoor air pollution can be said to be a problem caused by various pollutants generated by human activities being released into the room and polluting the indoor environment. There is a possibility of physical, chemical, and biologically diverse contaminants in the indoor air, and these pollutants are complex sources of inflow of external air, cigarette smoke, heaters, ovens, cookware, cement, cleaning agents, building materials, paints, etc. It can be said to be due to.

Research on indoor air quality in developed countries has progressed from research on actual conditions to research on indoor air quality predictive modeling. Recently, although many studies on health risk assessment for multi-use facilities have been conducted, domestic indoor Research on air quality has been conducted in various ways based on this law since the Ministry of Environment's “Indoor Air Quality Management Act of Multi-Use Facilities, etc.” was financed and implemented in 2003 and 2004. It is the reality that problems related to are newly raised.

Currently, research on indoor air quality is mainly focused on research on volatile organic compounds, while standards and studies on total suspended bacteria are still incomplete, and further studies including identification of microorganisms and fungi, etc. There is no research on risk studies focusing on the health of microbial contamination.

New industrial harmful substances (formaldehyde, tobacco smoke, volatile organic compounds, radon, asbestos, etc.) are constantly appearing due to the development of industrial and analytical technologies and improved living standards. It is a semi-volatile organic compound and fine dust. Recently, the importance of microbial contamination in indoor air has been greatly emphasized, such as the concept of immune buildings to cope with indoor microbial contamination.

In addition, recent infections in the Middle East respiratory syndrome (MERS, MERS), which are causing a major social problem in Korea, also affect droplets infection caused by pathogens mixed with saliva or sputum spreading when an infected person coughs through the respiratory tract. Concerns are growing. Tuberculosis, epidemic virus, pneumonia, norovirus, etc. are typical diseases that are transmitted by droplet infection, and they do not float in the air and scatter short distances of about 1m, so droplet infection occurs when they come into close contact. In this connection, there is a growing concern about air infection, and air infection refers to causing infection by inhaling microorganisms attached to particles of 5 microns or less and scattering them according to the flow of air for a long time. Unlike droplet infection, which requires close contact, there is a risk of infection even in patients at a distance, and as a result, the importance of indoor air quality is more important than ever. A multi-use facility is a facility used by a large number of unspecified people, including underground stations, underground shopping malls, terminals, museums, exhibition halls, libraries, general hospitals, and movie theaters.

In addition to underground stations and underground shopping malls, domestic multi-use facilities are defined as 21 facility groups such as childcare facilities, medical institutions, jjimjilbangs, and large-scale stores. Recently, public transportation vehicles such as railroad cars and intercity buses have been established.'14. 3. Effective from the 23rd, a total of 22 facility groups are included in the management target. Since there are various causes of indoor air pollution in a multi-use facility, it is necessary to properly manage it according to the characteristics of each facility, and includes common management such as proper ventilation facility operation and periodic cleaning, and indoor smoking ban. Sick Building Syndrome (SBS) refers to the irritation of eyes, nose and throat, dry mucous membranes and skin, erythema or erythema, mental fatigue and headaches, respiratory infections and coughing, irritability, etc. Symptoms appear in more than 30%. Modern people who live more than 80-90% of the day indoors are consciously or unconsciously exposed to indoor air pollutants due to various causes. Especially, multi-use facilities do not regularly manage air quality because many people gather and work. If it is not, proper indoor air quality management is important because it may cause respiratory diseases and atopy caused by bacterial propagation. Bacteria, etc. can multiply through the use of a hospital air conditioner or disinfectant spray, and tuberculosis, pneumonia, etc. can be moved through the hospital's ventilation system to promote disease occurrence in the hospital. Recently, in Korea, a number of problems related to serious hospital infections and infection paths have been exposed in Korea's representative hospitals in relation to MERS, and the reason for the rapid spread of MERS in this regard is the system that circulates through the air conditioner and exits the hospital It is thought that there is also a possibility that an infected person may develop under the condition. Therefore, it is paying attention to the importance of indoor air quality management in the park.

The contents of the antifungal and antimicrobial composition related to the present invention are described in Patent Nos. 10-0632859, 10-1808912, etc., and will be described later in comparison with the present invention.

Accordingly, an object of the present invention is to provide a composition for disinfection of natural ingredients usable in a multi-use facility.

The present inventors confirmed that the composition containing Cinnamon, Pulegone, Geranial, Cineol, Menthol and cypress as active ingredients shows excellent antifungal and antibacterial effects in a multi-use facility and completed the present invention.

That is, the present invention,

Cinnamon and Pulegone 1 to 15 parts by weight, Geranial 1 to 30 parts by weight, Cineol 1 to 20 parts by weight, Menthol 5 to 35 parts by weight and cypress (Chamaecyparis obtusa) provides an antimicrobial, antifungal and insecticidal composition for disinfection in a multi-use facility characterized by comprising 1 to 15 parts by weight of essential oil.

Disinfection in a multi-use facility characterized by being prepared by mixing the composition of claim 1 at a concentration of 5 to 40 (V/V)% in a lower aliphatic alcohol solvent having 1 to 3 carbon atoms having a purity of 95 to 99 (V/V)%. Provides antibacterial, antifungal and pesticide disinfectants.

Cinnamon and Pulegone 1 to 15 parts by weight, Geranial 1 to 30 parts by weight, Cineol 1 to 20 parts by weight, Menthol 5 to 35 parts by weight and cypress (Chamaecyparis obtusa) to prepare a composition comprising 1 to 15 parts by weight of essential oil,

Antibacterial and antifungal for disinfection in a multi-use facility, characterized in that the composition is mixed at a concentration of 5 to 40 (V/V)% in a lower aliphatic alcohol solvent having 1 to 3 carbon atoms having a purity of 95 to 99 (V/V)%. And pesticide disinfectants.

The present invention was a composition containing Cinnamon, Pulegone, Geranial, Cineol, Menthol and cypress as active ingredients, and provided a disinfectant showing almost perfect antifungal and antibacterial effects in a multi-use facility. In addition, the composition has excellent harmful insecticidal effect, and can provide a pleasant environment with natural ingredients.

1 is a diagram showing the activity time of a single person.
2 is a photograph showing a microbial monitoring scene of a multi-use facility for each region.
3 is a microbial culture picture obtained as a result of monitoring the microorganisms in the multi-use facility for each region.
Figure 4 shows the morphological characteristics of some fungi isolated from the cultured microorganisms.
5 shows morphological characteristics of some bacteria isolated from cultured microorganisms.
6 is a table of antifungal properties of the composition of the present invention against fungi and other compositions.
7 is a table of antimicrobial activity of the composition of the present invention and other compositions against bacteria collected in a multi-use facility.
8 is a table of antifungal properties of the composition of the present invention and other compositions against mold collected in a multi-use facility.
9 is a table showing the pesticidal effect of the composition of the present invention against pests.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

4 times the number of colonies obtained by collecting and culturing for 2 minutes and 30 seconds in a way that does not fix the sample collection location at department stores, underground shopping malls, hospital stations, terminals, etc. in Seoul, Daejeon, Daegu, etc. Was calculated as the final value, and repeated measurements were performed three times for each place using a room temperature medium, and temperature and humidity were measured to separate/identify floating microorganisms floating in the air.

(1) Distribution of aerial microorganisms

To collect microorganisms floating in the air in a multi-use facility, a pre-prepared medium (Potato dextrose agar) was installed and used in an air collector (MAS 100 Air sampler, Germany). The air filtration speed was 100 L/min, and the suction speed (the speed at which microorganisms in the air hit the surface of the agar) was 11 m/sec, and the microorganisms were collected for 10 minutes. The collected microorganisms were cultured for 7 days in a 25±1°C incubator to observe the colony number of microorganisms and morphological characteristics of harmful microorganisms.

(2) Pure separation of microorganisms within a multi-use facility

Microorganisms floating in the air were separated from department stores, underground shopping malls, hospital stations, and terminals, which are multi-use facilities such as Seoul, Daejeon, and Daegu, by region.

(3) Identification of isolated microorganisms Ribosomal DNA ITS sequence analysis

The strains were inoculated into PDB (Potato Dextrose Broth) and shaken and cultured at 25°C for 3 days to harvest mycelium. After lyophilizing the mycelium in a 1.5 ml microtube, genomic DNA was extracted by Lee & Taylor (1990). Primers ITS1 (5'-TCC GTA GGT GAA CCT GCG G-3') and ITS4 (5'-TCC TCC GCT TAT TGA TAT GC-3 used by White et al. (1990) to amplify the rDNA-ITS region of the known strain. ') was used. For amplification of the ITS region, 3 μl of 2.5 mM dNTP, 5 μl of 10X buffer, 0.4 μl of 100 uM primer, 0.3 μl of 1.5 unit/µl Taq DNA polymerase (SolGent Co., Ltd.), and 1 μl of genomic DNA mixed with sterile water The final volume was 50 µl. PCR conditions were repeated 35 times after predenaturation at 95°C for 4 minutes, denaturation at 95°C for 1 minute, annealing at 58°C for 1 minute, extension at 72°C for 35 minutes, and extension at 7°C for 7 minutes for ITS1, 5.8S, and ITS2 regions. And a single band of about 570 bp, including some 18S and 28S regions, was amplified. The amplified gene was purified with PCR ₉₆ Cleanup Plates (Millipore Corp. Bedford., MA 01730) and then subjected to sequencing PCR reaction using BigDye Terminator v3.1 Cycle Sequence Kit (ABI 0401041). Was used. After sequencing PCR product was purified with Montage SEQ ₉₆ Sequencing Reaction Cleanup Kit (Millipore Corp., LSKS 096 24), the base sequence was determined using an ABI 3100 DNA Sequencer.

(4) Antibacterial activity (Halo Test) measurement

To measure the antibacterial activity, first, the culture was completed for 2 days, followed by centrifugation of the culture medium for 4 days at 3,000 rpm for 15 minutes, and the supernatant and the cells were separated to perform an in vitro antibacterial activity assay for fungi. For the antibacterial activity assay, a middle layered black plate was prepared and used with PDA. The preparation of the middle layered flat plate was poured into 20 ml of a petri dish and solidified to form a lower layer, and shaken or stationary cultured strains were inoculated in the same medium to make 4 to 5 ml as a middle layer on the lower layer. To measure the antibacterial activity, spores prepared from the slant cultured for 7 days were spread on a petri dish so that 10 ⁶ ∼ 10 ⁷ cells/ml, and then 10ul of various samples were added to a paper disc (Φ6mm, Advantec Co.) and placed on it. (Mold) was cultured for 48 hours. The antimicrobial activity was confirmed by measuring the presence or absence and diameter of the stopper.

The composition for disinfection of the present invention is Cinnamon, 1 to 15 parts by weight of Pulegone, 1 to 30 parts by weight of Geranial, 1 to 20 parts by weight of Cineol, and menthol (Menthol) ) 5 to 35 parts by weight and 1 to 15 parts by weight of cypress (Chamaecyparis obtusa) essential oil, and the composition in a lower aliphatic alcohol solvent having a purity of 95 to 99 (V/V)% of 1 to 3 carbon atoms 5 to 40 (V) /V)% concentration.

That is, Cinnamon and Pullegone (Pulegone) 1 to 15 parts by weight, Geranial (Geranial) 1 to 30 parts by weight, Cineol (Cineol) 1 to 20 parts by weight, Menthol (Menthol) 5 to 35 parts by weight And cypress (Chamaecyparis obtusa) antibacterial and antifungal compositions for disinfection in a multi-use facility composed of a mixture containing 1 to 15 parts by weight of essential oils were applied to the culture medium to confirm almost perfect antibacterial and antifungal properties, and the results were compared with other compositions. It was put on the table.

In the above, cinnamon and fulgon may be included in the composition in similar amounts to each other.

As shown in Table 1 in Figure 6, it was confirmed that the composition of active ingredients of Cinnamon, Pulegone, Geranial, Cineol, Menthol and Cypress is superior to other existing patent substances.

In addition, as shown in Table 2 of FIG. 7, the antimicrobial activity of the composition of the present invention and other compositions against bacteria collected in a multi-use facility was shown as a comparison table.

Table 3 of Figure 8 can be confirmed the excellent antifungal power of the composition of the present invention as a table of antifungal properties of the composition of the present invention and other compositions against the fungus collected in a multi-use facility.

(6) insecticidal effect test

Regarding pests that cause disease and inhabit pests that cause biological damage to cultural property artifacts, 10 pests are placed in a certain pest cage and 100ul of the disinfectant composition is added and checked for 24 hours. The insecticidal effect was compared by confirming. 9 shows a pesticidal effect of the composition of the present invention against pests.

As described above, the disinfectant prepared with the composition of the present invention showing almost perfect antibacterial, antifungal, and insecticidal effects can be used in various ways in a facility used by an unspecified number of people. For example, it can be used as a cleaning agent, a disinfectant spraying agent combined with an air purifier, an escalator cleaner, a periodic disinfecting agent, a liquid fragrance, and an impregnated fragrance for porous materials.

The rights of the present invention are not limited to the embodiments described above, but are defined by the claims, and those skilled in the art can make various modifications and adaptations within the scope of the claims. It is self-evident.

Claims (3)

In order to exert antifungal, antibacterial and insecticidal effects against fungi, bacteria, and harmful pests collected in a multi-use facility, Cinnamon and Pulegone 1 to 15 parts by weight, Geranial ( Geranial) 1 to 30 parts by weight, Cineol (Cineol) 1 to 20 parts by weight, Menthol (Menthol) 5 to 35 parts by weight, and cypress (Chamaecyparis obtusa) essential oil 1 to 15 parts by weight in a multi-use facility Antibacterial, antifungal and pesticidal compositions for disinfection. Disinfection in a multi-use facility characterized by being prepared by mixing the composition of claim 1 at a concentration of 5 to 40 (V/V)% in a lower aliphatic alcohol solvent having 1 to 3 carbon atoms having a purity of 95 to 99 (V/V)%. Dragon antibacterial, antifungal and pesticide disinfectants. In order to exert antifungal, antibacterial and insecticidal effects against fungi, bacteria, and harmful pests that are collected in multi-use facilities,
Cinnamon and Pulegone 1 to 15 parts by weight, Geranial 1 to 30 parts by weight, Cineol 1 to 20 parts by weight, Menthol 5 to 35 parts by weight and cypress (Chamaecyparis obtusa) to prepare a composition comprising 1 to 15 parts by weight of essential oil,
Antibacterial and antifungal for disinfection in a multi-use facility, characterized in that the composition is mixed at a concentration of 5 to 40 (V/V)% in a lower aliphatic alcohol solvent having 1 to 3 carbon atoms having a purity of 95 to 99 (V/V)%. And pesticide disinfectants.

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