KR102119513B1 - OsMYB9 gene improving phosphate uptake efficiency in plant and uses thereof - Google Patents

Abstract

The present invention relates to the OsMYB9 gene and its use to enhance the phosphate absorption efficiency of plants, the gene encoding the rice-derived electron regulatory protein OsMYB9 ( Oryza sativa MYB9) enhances the phosphate absorption efficiency of plants, so the OsMYB9 gene in plants It can be used to develop a plant capable of improving the absorption rate of phosphate and controlling the stress of phosphate deficiency by regulating the expression of.

Description

OsMYB9 gene improving phosphate uptake efficiency in plant and uses thereof}

The present invention relates to the OsMYB9 gene and its use to enhance the phosphate absorption efficiency of plants.

Due to the rapid increase in population, food shortage is a global problem, and to overcome this, it is required to develop a high yielding ability food crop. In order to cultivate multiple food crops, it is necessary to supply sufficient nutrients to the crops. For this purpose, fertilizer is added. One of the main components of fertilizer is phosphoric acid. Phosphoric acid is one of the essential nutrients required by plants, and it plays an important role in all metabolism processes in plant cells, such as energy transmission, signal transmission, enzyme activity regulation, biosynthesis of metabolites, photosynthesis, and respiration. Phosphoric acid is also an important component of phospholipids and nucleic acids, so it is the nutrient most needed by plants along with nitrogen.

Although there is a lot of phosphoric acid in the earth's lithosphere, most of the phosphoric acid in the soil is mainly in the form of combined with organic matter that is not available to plants or metal ions such as iron or aluminum. It is present in small amounts. Therefore, phosphoric acid acts as a factor limiting crop productivity. Accordingly, the phosphoric acid necessary for the growth of the plant is supplied in the form of fertilizer, but most of the supplied phosphoric acid is immobilized in an unusable form by combining with organic substances or metal ions present in the soil, especially in the case of acidic soil. Because of the accumulated aluminum ions, more active phosphate immobilization occurs, so the phosphate effectiveness of the soil is lower.

As such, fertilizers treated to increase the effective phosphate concentration in soil required for crop growth are responsible for increasing agricultural production costs. In addition, fertilizer, which has not been absorbed due to excessive use of fertilizer, accumulates in the soil and causes acidification of the soil, adversely affecting the growth of crops, causing groundwater pollution and eutrophication of surface water. Pollution of agricultural environment not only brings about quantitative and qualitative deterioration in the production of agricultural products, but also pollutes the living environment and threatens people's health. This problem can be solved by reducing the amount of chemical fertilizer added. Currently, various organic fertilizers have been developed and used to replace chemical fertilizers, but many problems remain. The fundamental solution to this problem is to reduce the amount of fertilizer used by improving the phosphate absorption capacity of plants. Therefore, it is required to develop crops that can be efficiently grown even in conditions of phosphoric acid deficiency.

On the other hand, research to improve the efficiency of nutrient utilization of crops has been carried out on rice, one of the most important food crops in the world, and a lot of efforts have been made to secure and preempt useful genes after the entire gene sequence of rice has been revealed. Is being done. Research on the absorption of inorganic nutrients from plants has been conducted for a long time in the field of cultivation and plant physiology, but recently, based on the molecular-level basic research on the absorption of inorganic nutrients, it uses genes related to absorption of inorganic nutrients. Research is being conducted worldwide to increase crop growth and yield, and to breed crops that can grow even under extremely limited nutrient conditions. In addition, the plant promotes the growth of roots and the formation of root hairs in a condition lacking phosphate, thereby increasing the surface area and improving the ability to absorb phosphate by inducing the expression of genes related to high affinity phosphate transporters, dephosphorylation enzymes and organic acid biosynthesis and secretion. And increase phosphoric acid availability.

Accordingly, in the present invention, the function of the MYB-CC type transcription control gene OsMYB9 derived from rice expressed in the phosphate deficiency condition is analyzed, and the plant capable of adapting to the phosphate deficiency condition by enhancing the phosphate absorption efficiency of the plant using the gene is analyzed. I want to develop.

Meanwhile, Korea Patent Registration No. 1546728 discloses and "OgPT gene to promote acid-absorbing efficiency of a plant and use thereof, is disclosed, Korea Patent Registration No. 1.06459 million heading" rice dephosphorylation enzyme gene expressed in phosphate deficiency conditions OsPAP1 and Transgenic Plants' have been disclosed, but no description has been made of the OsMYB9 gene and its use to enhance the phosphate absorption efficiency of plants of the invention.

The present invention was derived by the above-described demands, and in the present invention, the present invention was completed by confirming that the OsMYB9 ( Oryza sativa MYB9) gene is overexpressed in the stem and root of the rice plant under phosphate-deficient conditions.

In order to solve the above problems, the present invention provides a transcription control protein OsMYB9 ( Oryza sativa MYB9) derived from rice.

In addition, the present invention provides a gene encoding the OsMYB9 protein.

In addition, the present invention provides a recombinant vector containing the gene.

In addition, the present invention provides a host cell transformed with the recombinant vector.

In addition, the present invention provides a method of increasing the phosphate absorption efficiency of a plant compared to a wild type comprising the step of overexpressing the OsMYB9 gene by transforming the plant cell with a recombinant vector containing a gene encoding the OsMYB9 protein.

In addition, the present invention provides a method for producing a transgenic plant with improved phosphate absorption efficiency compared to a wild type using a recombinant vector containing a gene encoding OsMYB9 protein.

In addition, the present invention provides a transgenic plant with improved phosphate absorption efficiency prepared by the above method and seeds thereof.

In addition, the present invention provides a composition for enhancing phosphate absorption efficiency of a plant containing a gene encoding OsMYB9 protein.

The gene encoding the rice-derived electron-regulating protein OsMYB9 ( Oryza sativa MYB9) enhances the phosphate absorption efficiency of plants, and thus can be used to develop plants with improved phosphate absorption rates through regulation of the expression of OsMYB9 genes in plants. . In addition, plants with improved phosphoric acid absorption capacity can overcome the stress of phosphate deficiency, and thus, it is expected that agricultural production cost reduction and environmental pollution prevention effect can be obtained by reducing fertilizer input.

1 is a nitrogen (N), phosphoric acid (P), potassium (K), and iron (Fe) deficiency conditions of OsMYB9 gene expression pattern (A), rice ( Oryza sativa ) stem and root phosphate sufficient conditions (+P ) and the results shown phosphate deficiency conditions (-P) expression profile (B) and phosphate deficiency processing time OsMYB9 gene expression pattern (C) of the genes in OsMYB9.
Figure 2 is a SDS-PAGE results confirming the expression level of the GST tag protein and GST-OsMYB9 protein in E. coli transformants.
Figure 3 is a photograph showing the production process of OsMYB9 overexpressed transgenic rice plants. (A); Dongjin Rice Tooth, (B); Callus induction, (C) selection of embryogenic callus, (d); Callus finally selected, (e); Plant regeneration I and (f); Plant regeneration Ⅱ.
4 is a result of RNA blot analysis performed to confirm whether the OsMYB9 gene has been introduced and to select a transformant having a high expression level of the OsMYB9 gene.
Figure 5 is a wild-type rice (WT) plant and OsMYB9 gene overexpressing the transgenic rice plant ( OsMYB9 -OX ) is grown in phosphate sufficiency (High Pi; A) and phosphate deficiency (Low Pi; B) conditions, stem length (Shoot) length; C), root length (D), stem phosphate content (Pi in shoot; E) and root phosphate content (Pi in root; F).

In order to achieve the object of the present invention, the present invention provides a transcription control protein OsMYB9 ( Oryza sativa MYB9) derived from rice.

The range of OsMYB9 protein according to the present invention includes a protein having an amino acid sequence represented by SEQ ID NO: 2 isolated from rice ( Oryza sativa ) and a functional equivalent of the protein.

In the present invention, the term "functional equivalent" as a result of addition, substitution, or deletion of an amino acid, the amino acid sequence represented by SEQ ID NO: 2 at least 70% or more, preferably 80% or more, more preferably 90% As described above, more preferably, it has a sequence homology of 95% or more, and refers to a protein having substantially the same physiological activity as the protein represented by SEQ ID NO: 2. "Substantially homogeneous physiological activity" refers to an activity that enhances the absorption efficiency of phosphate. The present invention also includes fragments, derivatives and analogs of OsMYB9 protein.

In the present invention, the terms "fragment", "derivative" and "analogue" refer to a polypeptide that has a biological function or activity substantially the same as the OsMYB9 polypeptide of the present invention.

In addition, the present invention provides a gene encoding the transcription control protein OsMYB9 ( Oryza sativa MYB9).

The gene of the present invention is characterized by enhancing the phosphate absorption efficiency of plants, and includes both genomic DNA, cDNA and synthetic DNA encoding OsMYB9 protein. Preferably, the gene of the present invention may include a nucleotide sequence represented by SEQ ID NO: 1. In addition, homologs of the base sequences are included within the scope of the present invention. Specifically, the gene includes a nucleotide sequence having a sequence homology of 70% or more, more preferably 80% or more, more preferably 90% or more, and most preferably 95% or more, respectively, to the nucleotide sequence of SEQ ID NO: 1 can do. “% of sequence homology” to a polynucleotide is identified by comparing two optimally aligned sequences, and a portion of the polynucleotide sequence in the comparison region is a reference sequence (not including additions or deletions) to the optimal alignment of the two sequences. Comparison), or additions or deletions (ie, gaps).

In addition, the present invention provides a recombinant vector comprising the OsMYB9 gene according to the present invention. In the recombinant vector of the present invention, the OsMYB9 gene may preferably consist of the nucleotide sequence of SEQ ID NO: 1.

In the present invention, the term “recombinant” refers to a cell in which a cell replicates a heterologous nucleic acid, expresses the nucleic acid, or expresses a peptide, a heterologous peptide or a protein encoded by a heterologous nucleic acid. Recombinant cells can express genes or gene segments not found in the natural form of the cells, either in sense or antisense form. Recombinant cells can also express genes found in natural cells, but the genes have been modified and reintroduced into the cell by artificial means.

In the present invention, the term "vector" is used to refer to a DNA fragment(s), nucleic acid molecules that are delivered into a cell. Vectors replicate DNA and can be reproduced independently in host cells. The term "carrier" is often used interchangeably with "vector".

Vectors of the invention can typically be constructed as vectors for expression or cloning. In addition, the vector of the present invention can be constructed using prokaryotic or eukaryotic cells as hosts. For example, when the vector of the present invention is an expression vector, and a prokaryotic cell is used as a host, a strong promoter (for example, pLλ promoter, trp promoter, lac promoter, T7 promoter, tac promoter, etc.) that can progress transcription ), a ribosome binding site for initiation of translation and a transcription/detox termination sequence. When Escherichia coli is used as a host cell, a promoter and an operator site of the E. coli tryptophan biosynthetic pathway, and a leftward promoter (pLλ promoter) of phage λ can be used as a regulatory site.

In the recombinant vector of the present invention, the promoters are suitable for transformation, and may be a CaMV 35S promoter, an actin promoter, a ubiquitin promoter, a pEMU promoter, a MAS promoter, or a histone promoter, preferably a CaMV 35S promoter. However, it is not limited thereto.

In the present invention, the term "promoter" refers to the region of the DNA upstream from the structural gene and refers to a DNA molecule to which RNA polymerase binds to initiate transcription. "Plant promoter" is a promoter capable of initiating transcription in plant cells. A "constitutive promoter" is a promoter that is active under most environmental conditions and developmental status or cell differentiation. Constitutive promoters may be preferred in the present invention because selection of transformants can be made by various tissues at various stages. Thus, the constitutive promoter does not limit the possibility of selection.

The recombinant vector of the present invention can be constructed by methods well known to those skilled in the art. Such methods include in vitro recombinant DNA technology, DNA synthesis technology, and in vivo recombinant technology. The DNA sequence can be effectively linked to a suitable promoter in the expression vector to direct mRNA synthesis. In addition, the vector may include a ribosome binding site and a transcription terminator as a translation start site.

The recombinant expression vector may preferably contain one or more selectable markers. The marker is a nucleic acid sequence having a property that can be selected by a chemical method, and all genes capable of distinguishing transformed cells from non-transformed cells correspond to this. The marker gene may be an antibiotic resistance gene or an axotrophic marker gene, but is not limited thereto.

In addition, a host cell transformed with the recombinant vector of the present invention is provided. As the host cell capable of continuously cloning and expressing the vector of the present invention stably, any host cell known in the art can be used. Examples of prokaryotic cells include E. coli JM109, E. coli BL21, and E. coli. Bacillus strains such as RR1, E. coli LE392, E. coli B, E. coli X 1776, E. coli W3110, Bacillus subtilis, Bacillus thuringiensis, and Salmonella typhimurium, Serratia marsensons, and various others Intestinal bacteria and strains such as Pseudomonas species.

When transforming the vector of the present invention into eukaryotic cells, as a host cell, yeast ( Saccharomyce cerevisiae ), insect cells, human cells (e.g., CHO cell line (Chinese hamster ovary), W138, BHK, COS-7, 293, HepG2, 3T3, RIN and MDCK cell lines) and plant cells and the like can be used. The host cell is preferably a plant cell.

The method for transporting the vector of the present invention into a host cell includes, when the host cell is a prokaryotic cell, a CaCl ₂ method, one method (Hanahan, D., 1983 J. Mol. Biol. 166, 557-580) and electroporation. It can be carried out by a method or the like. In addition, when the host cell is a eukaryotic cell, the vector is injected into the host cell by microinjection, calcium phosphate precipitation, electroporation, liposome-mediated transfection, DEAE-dextran treatment, and gene balm treatment. Can be.

In addition, the present invention transforms plant cells with a recombinant vector containing a gene encoding a transcription-derived protein OsMYB9 ( Oryza sativa MYB9) derived from rice and over-expresses the OsMYB9 gene, thereby phosphate absorption efficiency of plants compared to the wild type. It provides a way to increase.

In one embodiment of the present invention, the OsMYB9 protein may be composed of the amino acid sequence of SEQ ID NO: 2, but is not limited thereto.

When transforming the vector of the present invention into a plant cell, the host cell may preferably be a plant cell of Poaceae , more preferably a rice cell, but is not limited thereto. The method for transforming the plant cells is as described above.

In addition, the present invention comprises the steps of transforming plant cells with a recombinant vector containing a gene encoding a transcription control protein OsMYB9 ( Oryza sativa MYB9) derived from rice; And

Provided is a method for producing a transformed plant with increased phosphate absorption efficiency compared to a wild type comprising the step of re-differentiating the transformed plant from the transformed plant cell.

In one embodiment of the present invention, the OsMYB9 protein may be composed of the amino acid sequence of SEQ ID NO: 2, but is not limited thereto.

The method for transforming the plant cell is as described above, and the method for re-differentiating the transformed plant from the transformed plant cell can use any method known in the art.

In addition, the present invention provides a transgenic plant with improved phosphate absorption efficiency prepared by the above method and seeds thereof.

In one embodiment of the present invention, the plant used in the present invention is a monocotyledonous plant such as rice, barley, wheat, rye, corn, sugar cane, oat, onion, or Arabidopsis thaliana, potato, eggplant, tobacco, pepper, tomato , Burdock, garland chrysanthemum, lettuce, bellflower, spinach, beetroot, sweet potato, celery, carrot, buttercup, parsley, cabbage, cabbage, radish, watermelon, melon, cucumber, pumpkin, gourd, strawberry, soybean, mung bean, kidney bean, pea, etc. It may be a dicotyledonous plant, preferably a monocotyledonous plant, more preferably a Poaceae plant, and even more preferably a rice plant ( Oryza sativa ), but is not limited thereto.

In addition, the present invention provides a composition for increasing the phosphate absorption efficiency of a plant comprising a gene encoding the transcription control protein OsMYB9 ( Oryza sativa MYB9) derived from rice as an active ingredient, consisting of the amino acid sequence of SEQ ID NO: 2.

The composition includes a gene encoding the amino acid sequence of SEQ ID NO: 2 as an active ingredient or a recombinant vector containing the gene, and is capable of increasing the phosphate absorption efficiency of a plant by transforming the gene into a plant.

Hereinafter, the present invention will be described in detail by examples. However, the following examples are merely illustrative of the present invention, and the contents of the present invention are not limited to the following examples.

Example 1. A transcription control gene derived from rice ( OsMYB9 )

In the present invention, Dongjin rice ( Oryza sativa cv. Dongjin ), a cultivar of Korea, was used. Oligonucleotide primer (forward primer, 5'-CACCATGTATGAACCAAAGCC-3') (specific sequence number) specific to the MYB-CC type transcription control gene ( OsMYB9 ) derived from rice based on the whole genomic base sequence of rice by separating genomic DNA from the leaves of Dongjin rice 3) and reverse primer, 5'-TCAATCATCACTGCCTGAACC-3' (SEQ ID NO: 4)) were prepared, and the coding region of the gene was amplified using PCR method. After the amplified product was cloned into the pEntr vector, the nucleotide sequence of the gene was confirmed through sequencing. OsMYB9 , a transcriptional regulatory gene expressed under phosphate-deficient conditions, is a new gene that has not been previously identified. It has a base sequence of 990 bp in total and encodes 329 amino acids. The cDNA nucleotide sequence and amino acid sequence of the OsMYB9 gene are denoted by SEQ ID NO: 1 and SEQ ID NO: 2, respectively. As a result of comparing the homology between the OsMYB9 gene isolated in the present invention and the previously reported OsPHR1 and rice-derived OsPHR2 transcription control genes of MYB-CC, these genes had a homology of 59% and 54%, respectively. Was confirmed (data not shown).

Example 2. Paddy plants under conditions of phosphoric acid deficiency OsMYB9 Gene expression patterns

In general, since the expression of a gene having a similar function is regulated by tissue-specific or inducer-specific, the expression pattern of the isolated transcription control gene was analyzed using an RNA blot method. At this time, a primer having a specific nucleotide sequence of OsMYB9 gene transcription control gene was used as a probe.

Nitrogen affects the growth of hydroponic cultivation, the rice seeds in the dongjinbyeo two weeks distilled water _{(0.25mM (NH 4) 2 SO} 4, KNO 3), phosphoric acid (0.0125mM KH ₂ PO _4), potassium (0.01mM KNO ₃ ) And iron (0.01mM Fe-EDTA) component-deficient Hogland solution (200mM Ca(NO ₃ ) ₂ ·4H ₂ O, 2.5mM K ₂ SO ₄ , 2mM MgSO ₄ ·7H ₂ O, 1.25mM KH ₂ PO ₄ , 9 mM KCl, 0.25 mM MnSO ₄ , 0.25 mM H ₃ BO ₃ , 0.25 mM ZnSO ₄ , 0.25 mM CuSO ₄ , 0.25 mM Na ₂ MoO ₄ , 20 mM Fe-Sequestrate and 0.5 mM K ₂ SO ₄ , pH 5.5) for 1 week.

In addition, in order to confirm the expression pattern of the phosphate deficiency time-dependent gene for the stem and root, 1 in each of the Hogland solution treated with a sufficient phosphate condition (1.25 mM KH ₂ PO ₄ ) and a phosphate deficiency condition (0.0125 mM KH ₂ PO ₄ ). After incubation for a week, stem and root samples were collected, and RNA was isolated from the collected stem and root samples using Trizol reagent (Sigma). 10 µg of the separated RNA was loaded onto a 1.2% (w/v) denaturing formaldehyde agarose gel and electrophoresed, and then RNA was attached to a nylon membrane (Amersham). The RNA-attached nylon membrane was [ ³² P] dCTP labeled with 20% (w/v) SDS, 20×SSPE, 100 g/L PEG (8,000 mwt), 250 mg/L heparin and 10 ml/ The expression pattern of the OsMYB9 gene was confirmed by reacting with a solution containing L herring sperm DNA (10 mg/ml) overnight at 65°C.

As a result, when the nitrogen or potassium deficiency in was observed to be only significantly increased the expression of OsMYB9 gene if not OsMYB9 not gene is expressed phosphate and iron deficiency, particularly if phosphoric acid is deficient in more expression of the OsMYB9 gene It was confirmed to increase (Fig. 1A). In addition, the expression level of OsMYB9 gene was not high in both stalk and root conditions under sufficient phosphate, but it was found that the expression level of OsMYB9 gene was increased in phosphate deficiency condition (FIG. 1B ). And phosphate deficiency processing time (0, 1, 3, 5 and 7) as a result of confirming the OsMYB9 gene expression level in stems and roots, OsMYB9 genes increase the amount of expression over time and started to express from phosphate deficiency initial It was confirmed (Fig. 1C).

Example 3. Rice plant OsMYB9 OsMYB9 protein expression analysis using recombinant vector containing gene

The OsMYB9 gene was cloned into the pGEM-T easy vector, and the DNA fragments shown by treating the restriction enzymes Hind III and Xho I, respectively, were linked downstream of the GST tag of the protein expression vector peT42a vector to prepare a preparation vector. After transforming E. coli BL21 (DE3) with the prepared vector, IPTG (Isopropyl-β-D-thio-galactoside) was added to 1 mM and cultured at 37° C. for 3 hours to induce protein expression. Electrophoresis was performed on the SDS-PAGE gel to confirm the expression of the recombinant protein.

As a result, it was confirmed that the GST tag protein and the OGST-sMYB9 protein were expressed, and through this, it was confirmed that the target protein OsMYB9 protein in E. coli transformants was successfully expressed (FIG. 2).

Example 4. OsMYB9 Analysis of phosphate content in transgenic rice plants overexpressing genes

The coding region of the OsMYB9 gene to produce a transgenic rice plants overexpressing OsMYB9 gene destination vector; construct was cloned in (destination vector pH7WG2D, 1) is controlled by the CaMV 35S promoter which is OsMYB9 gene strongly expressed truck site ( It made a construct), samchin mating (triparental mating) method with Agrobacterium tyumeo Pacific Enschede (Agrobacterium tumefaciens ) EHA105. A transgenic plant was produced using Agrobacterium tumefaciens EHA105 containing a construct in Dongjin rice callus (FIG. 3), and the amount of expression after confirming whether the OsMYB9 gene was introduced and the amount of OsMYB9 gene was expressed by RNA blot method Strong transformants were selected (Figure 4).

Seeds harvested from transgenic plants and wild-type (control) seeds were hydroponic for 2 weeks, then treated with phosphate deficiency (0.0125 mM KH ₂ PO ₄ ) and phosphate deficiency (1.25 mM KH ₂ PO ₄ ) for 1 week and cultured Growth status was compared and analyzed (FIGS. 5A and 5B). As a result of measuring the stem and root length of transgenic rice overexpressing the wild type and OsMYB9 gene, the stem length in phosphate- rich conditions was increased in the wild type compared to the transformant, and the root length was increased in the transformant compared to the wild type, In the phosphate-deficient condition, the stem length was increased in the transformant compared to the wild type, and the root length was increased in the wild type compared to the transformant, but there was no significant difference between the wild type and the transformant (FIGS. 5C and 5D ).

In addition, as a result of measuring the phosphate content of the stem and root of the transgenic rice overexpressing the wild-type and OsMYB9 gene, it was confirmed that the phosphate content of the over-expressing transformant was increased by 30% or more compared to the wild-type in both phosphate-rich and phosphate-deficient conditions. (Figs. 5E and 5F).

<110> Dong-A University Research Foundation For Industry-Academy Cooperation <120> OsMYB9 gene improving phosphate uptake efficiency in plant and uses thereof <130> PN18341 <160> 4 <170> KopatentIn 2.0 <210> 1 <211> 990 <212> DNA <213> Oryza sativa <400> 1 atgtatgaac caaagccctt ctcaagcatt gtactagctc acaacgatcc agtttcccac 60 aatcaacaaa ttgaacgtat taacaacaat gtagtatcta atagtggtgg gaacagctca 120 aacagcaact ttgccgccag acagcgctta cggtggactg atgacctcca tgatcgtttt 180 gtggatgctg taacacagct tggtggaccc gacagggcaa ctccgaaggg aattcttaga 240 ataatgggtg ttcaagggtt gaccatctat catgtcaaaa gccatctgca aaaatatcgg 300 cttgctaaat acattccaga tcccacagct gatggtgcca agtctgacaa gaaagatctc 360 ggagatttgc ttgctgacat tgaaagctcc tcgggaatgg aaatagggga ggctttaaag 420 ttgcagatgg aggtgcaaaa gcggctacat gaacaattag aggtgcaaag gcagctacag 480 ctccggatag aagcccaagg aaggtacctc cagaagatca ttgaggagca gcagcggctc 540 agtggtgtgc tgggtgaatc aggcaagtta ggcgccctgg gaccagctcc aggggagcca 600 taccaggatt ccaacaaaac cgacccctcg accccggtac cgacttccga atccccaatc 660 cgcgacaagg ctggaagcgg cttgttcaag accatttctt cgcacgacga ctgccgcgag 720 cctttgacac cggactccag ctgccgtgcc ggctcccctt tggagagccc ccccagggct 780 agcaagagga tccgggtcag cagcgacatt gatcatcgtg ggaacaatga gttcccccct 840 cctctcaaag ttccggagcc aagttcaggt tcagatttcc gacaggaaag ctcagtgtta 900 ttatcatcca gcgcagtgca cttcgattct ttggagagcc tggatgcaga tgaaaatgtc 960 ttcaccaatg gttcaggcag tgatgattga 990 <210> 2 <211> 329 <212> PRT <213> Oryza sativa <400> 2 Met Tyr Glu Pro Lys Pro Phe Ser Ser Ile Val Leu Ala His Asn Asp 1 5 10 15 Pro Val Ser His Asn Gln Gln Ile Glu Arg Ile Asn Asn Asn Val Val 20 25 30 Ser Asn Ser Gly Gly Asn Ser Ser Asn Ser Asn Phe Ala Ala Arg Gln 35 40 45 Arg Leu Arg Trp Thr Asp Asp Leu His Asp Arg Phe Val Asp Ala Val 50 55 60 Thr Gln Leu Gly Gly Pro Asp Arg Ala Thr Pro Lys Gly Ile Leu Arg 65 70 75 80 Ile Met Gly Val Gln Gly Leu Thr Ile Tyr His Val Lys Ser His Leu 85 90 95 Gln Lys Tyr Arg Leu Ala Lys Tyr Ile Pro Asp Pro Thr Ala Asp Gly 100 105 110 Ala Lys Ser Asp Lys Lys Asp Leu Gly Asp Leu Leu Ala Asp Ile Glu 115 120 125 Ser Ser Ser Gly Met Glu Ile Gly Glu Ala Leu Lys Leu Gln Met Glu 130 135 140 Val Gln Lys Arg Leu His Glu Gln Leu Glu Val Gln Arg Gln Leu Gln 145 150 155 160 Leu Arg Ile Glu Ala Gln Gly Arg Tyr Leu Gln Lys Ile Ile Glu Glu 165 170 175 Gln Gln Arg Leu Ser Gly Val Leu Gly Glu Ser Gly Lys Leu Gly Ala 180 185 190 Leu Gly Pro Ala Pro Gly Glu Pro Tyr Gln Asp Ser Asn Lys Thr Asp 195 200 205 Pro Ser Thr Pro Val Pro Thr Ser Glu Ser Pro Ile Arg Asp Lys Ala 210 215 220 Gly Ser Gly Leu Phe Lys Thr Ile Ser Ser His Asp Asp Cys Arg Glu 225 230 235 240 Pro Leu Thr Pro Asp Ser Ser Cys Arg Ala Gly Ser Pro Leu Glu Ser 245 250 255 Pro Pro Arg Ala Ser Lys Arg Ile Arg Val Ser Ser Asp Ile Asp His 260 265 270 Arg Gly Asn Asn Glu Phe Pro Pro Pro Leu Lys Val Pro Glu Pro Ser 275 280 285 Ser Gly Ser Asp Phe Arg Gln Glu Ser Ser Val Leu Leu Ser Ser Ser 290 295 300 Ala Val His Phe Asp Ser Leu Glu Ser Leu Asp Ala Asp Glu Asn Val 305 310 315 320 Phe Thr Asn Gly Ser Gly Ser Asp Asp 325 <210> 3 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 3 caccatgtat gaaccaaagc c 21 <210> 4 <211> 21 <212> DNA <213> Artificial Sequence <220> <223> primer <400> 4 tcaatcatca ctgcctgaac c 21

Claims (13)

delete delete delete delete delete Transcriptional control of a rice plant origin comprising the amino acid sequence of SEQ ID NO: 2 protein OsMYB9 (Oryza sativa MYB9) the by transforming a rice plant cell with a recombinant vector comprising a gene encoding the rice compared to the wild type comprises the step of overexpressing OsMYB9 gene Method for increasing the efficiency of absorption of phosphoric acid in plants. delete Transforming rice plant cells with a recombinant vector comprising a gene encoding a transcription-derived protein OsMYB9 ( Oryza sativa MYB9) derived from the amino acid sequence of SEQ ID NO: 2; And re-differentiating transformed plants from the transformed rice plant cells. delete A transgenic rice plant with increased phosphate absorption efficiency prepared by the method of claim 8. delete A transformed seed of the rice plant according to claim 10. A composition for increasing the phosphate absorption efficiency of a rice plant comprising a gene encoding the transcription-regulating protein OsMYB9 ( Oryza sativa MYB9) derived from rice, consisting of the amino acid sequence of SEQ ID NO: 2, as an active ingredient.

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