KR102117649B1 - Composition for Treating and Preventing Gingivoperiodontitis or Periimplantitis and Proxabrush Coated with the Composition - Google Patents

Abstract

The present invention is gingival periodontitis salt containing sodium ethylenediaminetetraacetate, cetylpyridinium chloride, octyl phenol ethoxylate, and sodium bicarbonate as active ingredients. Or it relates to a pharmaceutical composition for the treatment and prevention of peri-implantitis, the composition according to the present invention effectively removes bacteria causing gingivitis and peri-implantitis to alleviate inflammation, and the interdental toothbrush coated with the composition is between teeth, implants, It is easy to apply to orthodontic devices, etc., and has the effect of easily treating and preventing gingivitis and peri-implantitis at home.

Description

Composition for treating and preventing gingivitis or peri-implantitis and interdental toothbrush coated with the composition{Composition for Treating and Preventing Gingivoperiodontitis or Periimplantitis and Proxabrush Coated with the Composition}

The present invention relates to a composition for treating and preventing gingivitis or peri-implantitis and an interdental toothbrush coated with the composition, more specifically, sodium ethylenediaminetetra acetate, cetylpyridinium chloride, octyl A composition for treating and preventing gingivitis or peri-implantitis containing phenol ethoxylate, and sodium bicarbonate as an active ingredient, and an interdental toothbrush coated with the composition.

Periodontal disease (periodontal disease) is also commonly referred to as qichi, depending on the severity of the disease is divided into gingivitis (gingivitis) and periodontitis (periodontitis). Periodontitis is a relatively light and fast-recovering periodontal disease that is limited to the gums, ie, soft tissues, and gingivitis. Between the gingiva (gingiva) and the teeth, there is a V-shaped gap, where bacteria attack the lower part of the gum line of this sulcus, damaging the periodontal ligament and adjacent tissue. As inflammation progresses and more tissue is damaged, the groove develops into a periodontal pocket, and the deeper the periodontitis, the deeper the depth of the periodontal pocket. As the periodontal sap deepens, the periodontal ligament becomes inflamed and bone loss occurs, which can lead to loss of teeth. The cause of periodontal disease is a bacterial membrane called plaque formed on teeth, and the types of bacteria are Prevotella intermedia, Actinomyces israelii , Fusobacterium nucleatum and the like.

On the other hand, peri-implantitis is an inflammation of the gums and gingival bones around the implant. As the aging society increases, the number of implants is also increasing, and the incidence of peri-implantitis is also increasing. In the early stages of peri-implantitis, inflammation occurs only in the gums around the implant, and when it becomes severe, symptoms similar to periodontitis in which the gum bones are absorbed appear. However, in the case of implants, there is no periodontal ligament and nerves in the natural teeth, so the inflammatory reaction occurs faster and more destructively than the natural teeth. The direct causative agent of peri-implantitis is similar to the bacteria found in periodontitis.

In order to treat such periodontal disease and peri-implantitis, efforts are being made to combat causative bacteria that form the microbial membrane by taking antibiotics, but there is a problem that it cannot be used in practical clinical practice due to antibiotic-resistant bacteria generated during long-term use. To overcome this, various methods have been developed, such as the use of a fluorine compound or the use of an automatic tooth cleaning device, but have not achieved great results in terms of effectiveness. In addition, in the case of chlorhexidine, which is widely used in addition to antibiotics, since it is made of a liquid, the duration of the effect is short, and when used for a long time, there is a disadvantage of causing discoloration in teeth.

Treatment methods for regeneration of tissues destroyed by periodontal disease and removal of bacteria include calculus removal and root surface lubrication. However, after mechanical cleaning, a layer of bacteria is formed on the root surface, which inhibits fibroblast adhesion and connective tissue adhesion, and is provided as a substrate for bacteria to proliferate, which can interfere with the healing of periodontal tissue. Research on treatment methods has been conducted (KR 10-1453206B1). As a medicine that removes toxic substances derived from bacteria and bacteria during mechanical treatment, citric acid, fibronectin, tetracycline hydrochloride (Tc-HCl), phosphoric acid, and stannous fluoride), ethylene diamine tetraacetic acid (EDTA), chlorohexidine, formalin, cetylpyridinium chloride, sodium-N-lauroyl sarcosine , Zinc iontophoresis, etc. are being studied.

However, in the case of a conventional bactericidal agent (for example, KR 10-0621191, KR 10-0978822 B1), its liquidity is acidic, causing irritation to the gums, and it is developed as a patient visiting a hospital and being treated by a doctor. As a result, it was difficult for the patient to continuously use it for maintenance purposes. Currently, gingivitis and peri-implantitis symptom relief and prevention agents that can be used at home are mostly general toothpastes and gargles, and their effectiveness and persistence are not large. Periodontal disease is difficult to treat if not dealt with in the early stages, so it is important to manage and prevent the usual, so there is a need for a drug that can prevent gingivitis and peri-implantitis while the patient can easily use it at home as well as relieve symptoms.

Accordingly, the present inventors developed a composition capable of alleviating and preventing gingivitis and peri-implantitis symptoms as a result of diligent efforts to solve the problems of the prior art, and interdental toothbrush coated with the composition in a convenient form for use by patients after brushing. Developed. When the composition and the interdental toothbrush coated with the composition were used clinically, the present invention was completed by confirming the effect of alleviating gingivitis and peri-implantitis.

KR 10-1453206 B1 KR 10-0621191 B1 KR 10-0978822 B1

An object of the present invention is to provide a composition effective for alleviating and preventing symptoms of gingivitis and peri-implantitis, the use of the composition, and a method of treating and preventing the symptoms using the composition.

In order to achieve the above object, the present invention is sodium ethylenediaminetetraacetate (sodium ethylenediaminetetra acetate), cetylpyridinium chloride (cetylpyridinium chloride), octyl phenol ethoxylate (octyl phenol ethoxylate), and sodium bicarbonate (sodium bicarbonate) Provided is a pharmaceutical composition for treating and preventing gingivitis or peri-implantitis containing as an active ingredient.

The present invention also provides an interdental toothbrush coated with the composition.

The present invention also provides an implant crown coated with the composition.

The present invention also provides an orthodontic device coated with the composition.

The composition according to the present invention effectively relieves inflammation by removing bacteria causing gingivitis and peri-implantitis, and the interdental toothbrush coated with the composition is easy to apply between the teeth, implants, orthodontic devices, etc. And it can treat and prevent peri-implantitis, and the composition can be applied directly to the affected area as well as the implant crown and orthodontic device, so that the composition can act on the affected area for a longer period of time compared to the method applied directly to the affected area. The mitigation effect can be improved. In addition, there are bacteria that cause inflammation, but it is possible to remove bacterial gingivitis and peri-implantitis by removing bacteria from the implant crown and the orthodontic appliance that are not wiped by brushing.

1 is a photograph of a composition effective for alleviating and preventing symptoms of gingivitis and peri-implantitis according to the present invention and an interdental toothbrush coated with the composition.
Figure 2 is a photograph showing that the bacteria were removed by applying the composition according to an embodiment of the present invention to the crown mounted on the implant contaminated by bacteria.
Figure 3 is a clinical picture showing the effect of reducing the swelling and bleeding symptoms of the gums using the composition according to an embodiment of the present invention to the implant fixture.
4 is a clinical picture showing the effect of removing the bacteria around the orthodontic appliance and alleviating gum inflammation after applying the composition according to an embodiment of the present invention to the orthodontic appliance.
5 is a clinical picture showing the effect of reducing gum inflammation when the interdental toothbrush coated with the composition according to an embodiment of the present invention is used at the bottom of the implant crown.
Figure 6 is a result showing the antimicrobial activity of the composition according to a temporary example of the present invention is an antimicrobial peptide is additionally added.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by a person skilled in the art to which the present invention pertains. In general, the nomenclature used herein and the experimental methods described below are well known and commonly used in the art.

In the present invention, a composition and a composition containing sodium ethylene diaminetetra acetate, cetylpyridinium chloride, octyl phenol ethoxylate, and sodium bicarbonate When the coated interdental toothbrush was used, the effect of alleviating the symptoms of peri-implantitis and the effect of alleviating gingivitis symptoms could be clinically confirmed.

Accordingly, the present invention is an effective component of sodium ethylenediaminetetraacetate, cetylpyridinium chloride, octyl phenol ethoxylate, and sodium bicarbonate from a consistent point of view. It relates to a pharmaceutical composition for the treatment and prevention of gingivitis or peri-implantitis containing.

Sodium ethylenediaminetetraacetate is a substance that forms chelation with divalent cations such as Ca ^{2 +} , Mg ²⁺ , Fe ^{2 +} , and Pb ^{2 +} at neutral pH, and reacts with calcium ions in dentin. It is possible to provide a place to promote the migration of fibroblasts by chemotaxis by exposing the fresh collagen substrate by removing the bacterial layer formed when the root surface glide is formed by forming a calcium complex salt, and maintaining a growth factor having biological activity. Therefore, in the present invention, sodium ethylenediaminetetraacetate (sodium ethylenediaminetetra acetate) has the effect of removing the mineral component present on the root surface and antibacterial effect.

Octyl phenol ethoxylate (C ₁₄ H ₂₂ O(C ₂ H ₄ O) _n ) is a nonionic surfactant, hydrophilic polyethylene oxide group and hydrophobic 4-(1,1,3,3-tetramethylbutyl )-phenyl group, and is widely used as a detergent in the biochemical field. Octyl phenol ethoxylate permeabilizes the cell membrane at 0.1 to 0.5% concentration and permeabilizes the bacterial cell wall at 1% concentration in immunostaining. Order. In the present invention, octyl phenol ethoxylate (octyl phenol ethoxylate) has the effect of removing the lipopolysaccharide (lipopolysaccharide, LPS). Other nonionic surfactants commonly used in this field include polyoxyethylene, Tweens, and spans, but as a result of testing the removal effect of lipopolysaccharide, octyl phenol ethoxylate It was found that the effect of removing lipopolysaccharide was the greatest.

In the present invention, cetylpyridinium chloride is a tetravalent ammonium compound, which has an effect of killing bacteria and microorganisms and reducing dental plaque and gum inflammation.

In the present invention, sodium bicarbonate (sodium bicarbonate) has the effect of pH adjustment and polishing action.

In the present invention, the composition for treating and preventing gingivitis or peri-implantitis is 100-30 parts by weight, 15-30 parts by weight of sodium ethylenediaminetetra acetate, octyl phenol ethoxylate 1 to 2 parts by weight, cetylpyridinium chloride, 0.01 to 1 part by weight, sodium bicarbonate (sodium bicarbonate) may be characterized in that it contains 1 to 10 parts by weight.

 When the content of sodium ethylenediaminetetraacetate is less than 15 parts by weight, the demineralizing effect is not large, and when it exceeds 30 parts by weight, the demineralizing effect does not increase, so there is no practical benefit of increasing the content. In addition, when the content of octyl phenol ethoxylate is less than 1 part by weight, the effect of removing lipopolysaccharide does not occur, and when it exceeds 2 parts by weight, the effect of removing lipopolysaccharide does not increase, so the content of There is no real benefit from the increase. When the content of cetylpyridinium chloride is less than 0.01 part by weight, there is no antibacterial effect, and when it exceeds 1 part by weight, the antibacterial effect does not increase, so there is no practical benefit due to the increase in content. In sodium bicarbonate, when the content is less than 1 part by weight, the effect on pH adjustment and abrasive action is not large, and when it exceeds 10 parts by weight, the teeth are abrasive, so there is no practical benefit due to the increase in content.

In the present invention, the composition may further contain an antibacterial peptide. The antibacterial peptide is from the group consisting of human alpha-defensin, human beta-defensin, cathelicidin, LL-37 and histitin. It may be characterized by being selected, but is not limited thereto.

Antimicrobial peptides are present in the human innate immune system, and bind to cell membranes and puncture, showing a wide range of antibacterial activity against bacteria, fungi and viruses (Brogden KA. Antimicrobial peptides: pore formers or metabolic inhibitors) in bacteria.Nat Rev Microbiol 2005; 3:238.50., Sørensen OE. et al. , Antimicrobial peptides in innate immune responses, Contrib Microbiol 2008; 15:61.77.). In addition, certain antimicrobial peptides also neutralize the activity of LPS (Rosenfeld Y, Papo N, Shai Y. Endotoxin (lipopolysaccharide) neutralization by innate immunity host-defense peptides, Peptide properties and plausible modes of action, J Biol Chem 2006;281:163643).

Antibacterial peptides are produced in various cells involved in infection, such as epithelial cells, neutrophils, and salivary glands. Among them, there are human defensin, cathelicidin, LL-37, and histtin, and the peptides have cations, and septicemia caused by Gram-negative and Gram-positive bacteria. It is known to prevent inflammation (Scott MG. et al ., Interaction of cationic peptides with lipoteichoic acid and Gram-positive bacteria., Infect Immun 1999;67:6445.53., Giacometti A. et al. , Potential therapeutic role of cationic peptides in three experimental models of septic shock., Antimicrob Agents Chemother 2002;46:2132.6.).

Meanwhile, the antibacterial peptide may be selected from the group consisting of antibacterial peptides represented by amino acid sequences of SEQ ID NOs: 1 to 4, but is not limited thereto. In this case, the antimicrobial peptide represented by the amino acid sequence of SEQ ID NO: 2 exhibits the best effect in antibacterial activity, and is particularly preferably used in the composition of the present invention.

SEQ ID NO: 1 peptide GKCST RGRKC CRRKK-NH2

SEQ ID NO: 2 peptide GKSST RGRKS CRRKK-NH2

SEQ ID NO: 3 peptide GKCST RGRKS SRRKK-NH2

SEQ ID NO: 4 peptide GKSST RGRKC SRRKK-NH2

In the present invention, the antimicrobial peptide is the antimicrobial peptide is based on 100 parts by weight of the ^{composition, 1 × 10 -5 ~ 1 ×} 10 -3 may be characterized in that it contains parts by weight of 1 × 10 which the antimicrobial ^{peptide This} is because the antimicrobial effect is not large when contained in less than ⁵ parts by weight, and the antibacterial effect is not increased when contained in excess of 1×10 ^-3 parts by weight, so that there is no practical benefit due to the increased content.

In the present invention, the composition may further contain one or more selected from the group consisting of propolis, xylitol, and protease, and may further include a propolis or xylitol to improve the sensory properties, or protein By adding a degrading enzyme, it is possible to increase the antibacterial effect and the lipopolysaccharide removal effect.

The composition of the present invention is a pharmaceutically acceptable carrier includes excipients (for example, starch, lactose, calcium carbonate, calcium phosphate, etc.), binders (for example, starch, gum arabic, carboxymethylcellulose, hydroxymethylcellulose, Crystalline cellulose, etc.), lubricants (e.g., magnesium stearate, talc, etc.), decomposition agents (e.g., carboxymethylcellulose calcium, talc synthetic aluminum silicate, etc.), diluents (e.g., water, vegetable oil, etc.) Or a mixture of two or more of these can be used.

The formulation of the composition according to the present invention is not particularly limited, but may be formulated as a powder, fine granules, liquid, powder, spray, ointment, or gel, and is most preferably formulated as a gel.

In another aspect, the present invention relates to an interdental toothbrush coated with the composition.

The interdental toothbrush may be characterized in that the composition is coated with 0.01 to 0.04 g per piece, and when the coating weight is less than 0.01 g, the coating is not properly applied to the bristles, and when it exceeds 0,04 g, excessive coating This is because the coating layer is peeled off.

In another aspect, the present invention relates to an implant crown coated with the composition.

The implant crown may be characterized in that it is coated with 0.05 ~ 0.2g.

In another aspect, the present invention relates to an orthodontic device coated with the composition.

The orthodontic device may be characterized in that it is coated with 0.01 ~ 0.1g.

In another aspect, the present invention relates to the treatment and prevention of gingivitis or peri-implantitis of the composition.

In another aspect, the present invention relates to a method for treating and preventing gingivitis or peri-implantitis using the composition or the interdental toothbrush coated with the composition.

One embodiment of the treatment and prevention method according to the present invention includes the steps of washing the composition between the teeth, between the teeth, around the implant, or around the orthodontic appliance, by applying the composition on a soft brush or with the interdental toothbrush coated with the composition. It can be characterized by. In this case, it may be characterized by washing for 1 to 3 times 1 to 2 times a day.

In another embodiment of the present invention, (a) coating the composition on the implant crown; And (b) placing a coated implant crown. In the present invention, the step (a) may be characterized in that the coating is performed prior to the first implant or before re-implantation of the implant crown separated from the patient having peri-implantitis. The composition may be coated with 0.05 to 0.2 g on the implant crown, but is not limited thereto.

Another embodiment of the present invention, (a) removing the implant crown crown; And (b) may be characterized in that it comprises the step of applying the surrounding of the implant crown is removed with the composition of claim 1. The composition may be applied to 0.05 ~ 0.2g around the implant crown is removed, but is not limited thereto. In this case, in order to minimize irritation of the inflamed area, it can be applied using a soft, fine brush.

In another embodiment of the present invention, (a) coating the orthodontic device with the composition; And (b) may be characterized in that it comprises the step of mounting the coated orthodontic appliance to the teeth. The composition may be coated with 0.01 to 0.1g on the orthodontic device, but is not limited thereto.

As another embodiment of the present invention, it may be characterized in that the coating the composition on the orthodontic device mounted on the tooth. The composition may be coated with 0.01 to 0.1g on the orthodontic device, but is not limited thereto.

In the present invention, the coating is a technique of sufficiently applying the composition of the present invention to a surface of an interdental toothbrush, implant crown, orthodontic appliance for a certain period of time, and is simply intended to coat an interdental toothbrush, implant crown, orthodontic appliance. There may be a method of immersing the site in the composition of the present invention for 1 second to 10 minutes, and in another method, the composition of the present invention may be used by using a brush or the like on the site to be coated of the interdental toothbrush, implant crown, orthodontic device. It can be used as a method of even application or application.

Example

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, it will be apparent to those skilled in the art that the scope of the present invention is not to be construed as limited by these examples.

Example  One: Gingivitis  And preparation of an effective composition for alleviating and preventing the symptoms of peri-implantitis

After dissolving 1.5 g of sodium carboxymethyl cellulose and 1 g of the flavoring agent L-menthol in 73.45 g of purified water at about 100° C. to prepare a primary mixture, 17 g acetyldiaminetetraacetate dihydrate (EDTA-2Na -2H2O) was added and secondarily mixed at about 100°C. 2 g of Triton X-100 (Sigma, USA), 1 g octyl phenol ethoxylate, 5 g of sodium bicarbonate, 1 g of Xylitol, and 0.05 g of cetylpyridinium chloride (CPC) The mixture was added to the mixed solution to prepare an effective composition for alleviating and preventing gingivitis and peri-implantitis symptoms and filling the syringe (FIG. 1).

Example  2: Gingivitis  And a composition effective for alleviating and preventing the symptoms of peri-implantitis. Interdental  Produce

The composition of dental cleanser interdental brush is 10ea/set. The weight of a set of dental cleanser interdental toothbrushes (10ea/set) was measured with a balance before coating with the composition of Example 1. After soaking the bristle part of the interdental toothbrush in a clean bench (BSC type) in an airtight container containing the composition of Example 1 so as to be completely immersed in the composition of Example 1, and then leaving the composition upside down to the extent that the composition does not fall, and then standing on a drying stand It was completely dried in a clean bench (BSC type). The weight of the dried interdental brush was measured with a balance to compare the weight before and after coating to confirm the coated amount.

Calculation of the amount of the composition coated on the interdental brush (10ea/set) number Before coating Interdental brush  Weight (g) After coating Interdental brush  Weight (g) Weight gain = amount coated (g) One 1.2697 1.4497 0.18 2 1.3209 1.493 0.1721 3 1.2752 1.4703 0.1951 4 1.2722 1.4396 0.1674 5 1.2741 1.4653 0.1912 6 1.2762 1.4075 0.1313 7 1.3217 1.4513 0.1296 8 1.2703 1.4125 0.1422 9 1.3206 1.4448 0.1242 10 1.2734 1.429 0.1556 11 1.3157 1.4588 0.1431 12 1.2699 1.4312 0.1613 13 1.3204 1.4622 0.1418 14 1.2762 1.4421 0.1659 15 1.3205 1.4305 0.11 16 1.2731 1.4671 0.194 17 1.2762 1.4421 0.1659 18 1.2829 1.45271 0.16981 19 1.3104 1.46121 0.15081 20 1.3147 1.4598 0.1451 Average 0.156821 (0.046846821 per interdental brush)

Experimental Example  One: Gingivitis  And bacteria removal effect of the implant crown by applying the composition effective for alleviating and preventing the symptoms of peri-implantitis

The implant crown was detached from the patient with peri-implantitis and the degree of bacterial contamination was observed using a disclosing solution. The composition of Example 1 was applied to the surface of the implant crown and after 1-2 minutes of standing, it was washed with brine. 2 is a photo before and after applying the composition to the implant crown. Before applying the composition, it was dyed red, but after washing with the composition applied, it was not stained with a tooth colorant, which proves that bacteria and contaminants were completely removed.

Experimental Example  2: Gingivitis  And patients with peri-implantitis applied with a composition effective for alleviating and preventing the symptoms of peri-implantitis. Clinical effect

The implant crown was removed from the patient who had the implant, and the implant crown was removed, and the composition of Example 1 was applied (FIG. 3). As a result of observing 2 days, 6 days, and 2 weeks after applying the composition, inflammation, swelling, and bleeding around the implant decreased over time. After 2 weeks of application of the composition, the inflammation was completely removed and the implant crown could be refitted. This demonstrates that the composition of Example 1 is effective against peri-implantitis.

Experimental Example  3: Gingivitis  And a patient with an orthodontic appliance applied with a composition effective for alleviating and preventing peri-implantitis symptoms. Clinical effect

The composition of Example 1 was applied to a patient with a large amount of gum inflammation and plaque among the patients with the orthodontic appliance attached (FIG. 4). When dyed around the orthodontic appliance with a toothbrushing agent, it was stained red. The composition of Example 1 was applied around the orthodontic appliance and rubbed using a micro brush, and the remaining composition was washed with a cotton swab and saline. One week later, when observed again, it was observed that plaque and inflammation were removed. This proves that the composition of Example 1 is effective in removing plaque and alleviating gum inflammation.

Experimental Example  4: Gingivitis  And a composition effective for alleviating and preventing the symptoms of peri-implantitis. Interdental brush  Of patients with peri-implantitis Clinical effect

The interplant toothbrush coated with the composition of Example 2 was applied to a patient who had implant periartitis among the patients who placed the implant (FIG. 5 ). When the patient used the interdental toothbrush coated with the composition of Example 2 for 6 days once a day at home, and observed after a week visit, the inflammation around the implant was removed. This proves that the interdental toothbrush coated with the composition of Example 2 is effective for peri-implantitis.

Experimental Example  5: antibacterial Peptide  Improved efficacy with addition

To further improve the efficacy by adding an antibacterial peptide to the composition of Example 1, the following peptide was used for this.

SEQ ID NO: 1 peptide GKCST RGRKC CRRKK-NH2

SEQ ID NO: 2 peptide GKSST RGRKS CRRKK-NH2

In order to confirm the effect of the composition to which the antibacterial peptide was added, an antibacterial experiment was conducted according to the liquid dilution method. Periodontal causative agent, Prevotella Intermedia was cultured in Tryptic soy broth. The above strains were cultured at 620 nm until the absorbance became 1, and then the cells were collected and used in the experiment. Diluted with PBS, 10 ⁵ -10 ⁷ bacteria per ml were spread on TSA (tryptic soy agar) plates and incubated at 37° C. for 24 hours. To measure the antimicrobial activity of the peptide using the liquid dilution method, 1 ml of distilled water was added to the microtube, and then 10 μl of the diluted fungus solution was added and 50 μl of the plate was plated and used as a control.

50 µg/ml, 100 µg/ml of the peptide of SEQ ID NO: 1, 50 µg/ml of the peptide of SEQ ID NO: 2, 100 µg/ml, and treated at 37° C. for 1 hour, followed by 50 µl plating to generate the number of colonies. Counted. In addition, the antimicrobial activity of the composition of Example 1 and the composition of Example 1 in which the peptide of SEQ ID NO: 1 was mixed and the composition of Example 1 in which the peptide of SEQ ID NO: 2 was mixed were measured in the same manner.

As a result, as shown in Figure 6, the peptide of SEQ ID NO: 2 was the highest antimicrobial activity when 100 ㎍ / ml, it was confirmed that the antimicrobial effect is better than when the same concentration of the peptide of SEQ ID NO: 1. In addition, it was confirmed that when the composition of Example 1 was used, the antimicrobial effect was further increased when the antimicrobial peptide was added, and in particular, the antimicrobial effect of the composition in which the peptide of SEQ ID NO: 2 was mixed was significantly increased.

Since the specific parts of the present invention have been described in detail above, for those skilled in the art, it is clear that such specific technology is only a preferred embodiment, and the scope of the present invention is not limited thereby. something to do. Therefore, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

<110> Nano Intelligent Biomedical Engineering Corporation. co. Ltd <120> Composition for Treating and Preventing Gingivoperiodontitis or          Periimplantitis and Proxabrush Coated with the Composition <130> P18-B181 <160> 4 <170> KoPatentIn 3.0 <210> 1 <211> 15 <212> PRT <213> Artificial Sequence <220> <223> Antimicrobial Peptide <400> 1 Gly Lys Cys Ser Thr Arg Gly Arg Lys Cys Cys Arg Arg Lys Lys   1 5 10 15 <210> 2 <211> 15 <212> PRT <213> Artificial Sequence <220> <223> Antimicrobial Peptide <400> 2 Gly Lys Ser Ser Thr Arg Gly Arg Lys Ser Cys Arg Arg Lys Lys   1 5 10 15 <210> 3 <211> 15 <212> PRT <213> Artificial Sequence <220> <223> Antimicrobial Peptide <400> 3 Gly Lys Cys Ser Thr Arg Gly Arg Lys Ser Ser Arg Arg Lys Lys   1 5 10 15 <210> 4 <211> 15 <212> PRT <213> Artificial Sequence <220> <223> Antimicrobial Peptide <400> 4 Gly Lys Ser Ser Thr Arg Gly Arg Lys Cys Ser Arg Arg Lys Lys   1 5 10 15

Claims (12)

Sodium ethylenediaminetetra acetate, Cetylpyridinium chloride, octyl phenol ethoxylate, sodium bicarbonate, and an antimicrobial peptide represented by the amino acid sequence of any one of SEQ ID NOs: 2 to 4 A pharmaceutical composition for the treatment and prevention of gingivitis or peri-implantitis, which has antimicrobial activity, causing the periodontitis causing bacteria. According to claim 1, With respect to 100 parts by weight of the composition, sodium ethylenediamine tetraacetate 15 to 30 parts by weight, octyl phenol ethoxylate 1 to 2 parts by weight, cetylpyridinium chloride 0.01 to 1 part by weight and sodium bicarbonate 1 Pharmaceutical composition for the treatment and prevention of gingivitis or peri-implantitis characterized by containing ~10 parts by weight. delete delete delete According to claim 1, wherein the antimicrobial peptide is a pharmaceutical composition for treating and preventing gingivitis or peri-implantitis, characterized in that it contains 1×10 ^-5 to 1×10 ^-3 parts by weight based on 100 parts by weight of the composition. Interdental toothbrush coated with the composition of claim 1. The interdental toothbrush according to claim 7, wherein the interdental toothbrush is coated with 0.01 to 0.04 g of the composition per piece. An implant crown coated with the composition of claim 1. The implant crown according to claim 9, wherein the composition is coated with 0.05 to 0.2 g of each composition. Orthodontic device coated with the composition of claim 1. The orthodontic appliance of claim 11, wherein the orthodontic appliance is coated with 0.01 to 0.1 g of each composition.

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