KR102086066B1 - Composition comprising streptomyces scopuliridis n29 strain, or culture broth thereof as active ingredient for weed control - Google Patents

Abstract

The present invention relates to a Streptomyces scopuliridis N29 strain, that is a new strain, and a use thereof. More specifically, Streptomyces scopuliridis N29 strain of the present invention and a culture filtrate thereof exhibit excellent herbicidal activity on weeds such as ragweed, thereby being usefully used for a weed control herbicide composition and a weed control method.

Description

COMPOSITION COMPRISING STREPTOMYCES SCOPULIRIDIS N29 STRAIN, OR CULTURE BROTH THEREOF AS ACTIVE INGREDIENT FOR WEED CONTROL}

The present invention relates to novel Streptomyces scopuliridis strains and the use of herbicides of these strains.

Weeds or weeds are not absolute, but are relative concepts that occur when humans start farming and are not suitable for time and place. Weeds are divided into plant and weeds, broadleaf weeds, and roomy weeds according to phyto- taxonomic and morphological classification.

In Article 2 (7) of the Act on the Conservation and Utilization of Biodiversity, a foreign organism is defined as `` an entity that is artificially or naturally introduced from a foreign country and is beyond its original origin or habitat. '' There are 387 species in the genus 43 and 204, reported annually (Kim, 2017). Of the foreign plants introduced in Korea, 14 species, including thorny plants, are designated as ecosystem disturbing plants, and 11 species are defined as `` a species that needs to be controlled or controlled by establishing management measures due to the high ecological risk and the risk of future risk. '' The risk assessment is Level 1 (Choi, 2017). Ecosystem disturbance Foreign plants cause problems such as simplification of vegetation, change of habitat environment, allergens caused by pollen, and they are rapidly spreading around our daily lives by moving along rivers and roads, and also accelerating invasion into farmland. It is true.

In this situation, however, there are few domestic studies in the areas of distribution, physiology, and ecology of foreign plants, and there are no control or development of effective management techniques. In the future, the disruption of ecosystems is expected to accelerate the spread of foreign plants, which is why it is urgently needed to develop technologies for preventing and removing them.

The largest damage among the ecosystem disturbance plants designated as 1st grade in Korea is known as ragweed. It occurs mainly in Ansan-si, Gyeonggi-do, Cheongwon-gun, Daegu Achievement Marsh, Busan Nakdong River, and Seorak-dong, Sokcho-si, Gangwon-do. The ragweed ( Ambrosia artemisiifilia L.) is a 1 year old herbaceous plant in summer, a native herbaceous plant native to North America and widely naturalized in Europe and Asia. Its stem is about 30 ~ 180 cm high and its flowers bloom in August ~ September. It is a copper plant. An average of about 3,000 grains per week are formed for breeding seeds, and they are dormant in nature, and seed germination is known to be higher at constant temperature than at constant temperature (Kim and Park, 2009).

In Korea, the control of ecosystem disturbance plants such as ragweed is mainly used for physical control or biological control, so it is not a fundamental elimination but a temporary decrease in population (Choi, 2017). Ecosystem disturbance plants that invade agricultural lands such as barbed gourd can be partially controlled by herbicide treatment such as linuron or simazine, and ecological disturbance plants that occur on roadside or non-farmland can be effectively controlled by treating glufosinate-ammonium or glyphosate. (Lee et al, 2007).

However, control of the use of organic synthetic herbicides is practically limited, as the main distribution of ecosystem disturbances is mainly around people's active living (Moon et al., 2008). In the meantime, as a means for spreading or eliminating ecosystem disturbances in Korea, physical methods such as mowing are mobilized mainly, which requires a lot of labor, cannot be completely eliminated, and because of its high regeneration and faster reproduction than the rate of removal. Proliferation is accelerating.

Ecosystem disturbance As an alternative to solve the practical limitations of organic synthetic herbicide treatment, natural-based herbicide-active materials are attracting attention, which is likely to express different action characteristics than conventional herbicides, depending on the nature of natural products. This is because there is a relatively high possibility of securing the safety of the environmental ecosystem (Dayan et al., 2009; Christy et al., 1993). In Korea, some studies have been attempted to develop eco-friendly removal technology of disturbed plants, but it is still rudimentary. The analysis on the status of research on foreign weeds published in the Korean Weed Society is mainly focused on the characteristics, distribution and development of foreign weeds. The research on the ecosystem perturbation technology includes researches on the selection and control of insects for biological control of weeds in maple leaf ragweed or ragweed (Lee et al., 2007; Na et al., 2008) and natural products. The research on the control effect of d-limonene on spiny gourd (Choi et al., 2012), etc., and control of maple leaf stalk by soil-treated herbicide treatment, and spiny gourd using natural herbicide chrysophanic acid or pelargonic acid It was presented as a poster about the response.

Ecological disturbances The discovery of natural products based candidates for plant control is possible from a variety of raw materials, but soil actinomycetes (Duke et al., 1996; Satoh et al., 1993) produce a wide variety of secondary metabolites, Interest (Joseph et al., 2012).

Soil actinomycetes Streptomyces scopuliridis KR-001 strain is a natural herbicide candidate material discovered by the Korea Research Institute of Chemical Technology. The activity against gourd was very good (Lee et al., 2013), and the secondary metabolite herbicidin produced by this strain was a candidate material with potential as a non-selective herbicide (Won et al. , 2015).

Thus, the present inventors produced N29, an improved strain of the parent strain Streptomyces S. pulpuliridis ( S. scopuliridis KR-001), and the culture filtrate of the N29 strain was used for sorghum in natural conditions as well as in greenhouse conditions. Confirmed that the control effect is very excellent, it was determined that it can be usefully used as a candidate material for environmentally friendly ragweed control completed the present invention.

It is an object of the present invention to provide a novel Streptomyces scopuliridis strain.

Still another object of the present invention is to provide a weed control herbicide composition containing any one or more selected from the group consisting of the strain and its culture filtrate as an active ingredient, and a method for controlling weeds using the composition.

In order to achieve the above object, the present invention provides a Streptomyces scopuliridis N29 strain deposited with KCTC 13057BP deposited at the Korea Research Institute of Bioscience and Biotechnology.

The present invention also provides a herbicide composition for weed control containing any one or more selected from the group consisting of Streptomyces scopulididis N29 strain deposited with Accession No. KCTC 13057BP, and a culture filtrate thereof as an active ingredient.

In addition, the present invention, weed control comprising the step of treating any one or more of the group consisting of Streptomyces scopulididis N29 strain, and its culture filtrate deposited with accession number KCTC 13057BP to weeds, seeds or habitats thereof. Provide a method.

The culture filtrate containing Streptomyces scopuliridis N29 strain deposited with accession number KCTC 13057BP of the present invention was treated with a significant killing force as a result of cultivation in greenhouse and packaging conditions against weeds in ragweed. Since it is shown, it can be usefully used as a herbicide composition for weed control, and a weed control method.

Figure 1 shows the effect of the initial foliage treatment in greenhouse conditions of the Streptomyces scopulididis N29 strain deposited with accession number KCTC 13057BP. CK (check) indicates a control, and indicates that the strain culture filtrate or its dilution was not treated.
Figure 2 shows the effect of mid-term foliage treatment in greenhouse conditions of the Streptomyces scopulididis N29 strain deposited with accession number KCTC 13057BP. CK (check) indicates a control, and indicates that the strain culture filtrate or its dilution was not treated.
Figure 3 shows the late foliage treatment effect in greenhouse conditions of the Streptomyces scopulididis N29 strain deposited with accession number KCTC 13057BP. CK (check) indicates a control, and indicates that the strain culture filtrate or its dilution was not treated.
Figure 4 shows the control effect on ragweed of the 3-4 leaf phase in the packaging conditions of Streptomyces scopulididis N29 strain deposited with accession number KCTC 13057BP. CK (check) indicates a control, and indicates that the strain culture filtrate or its dilution was not treated.
Figure 5 shows the control effect on the ragweed of 5-6 leaves in the packaging conditions of Streptomyces scopulididis N29 strain deposited with accession number KCTC 13057BP. CK (check) indicates a control, and indicates that the strain culture filtrate or its dilution was not treated.
FIG. 6 shows Streptomyces scopulididis N29 strain deposited with accession number KCTC 13057BP, Streptomyces scopulididis KR-001 strain and streptomyces scopulididis M40 deposited with accession number KCTC 12156BP The late mutant strain J. Microbiol.Biotechnol. The effect of foliage treatment is shown in comparison.

Hereinafter, the present invention will be described in detail.

The present invention provides Streptomyces scopuliridis N29 strain deposited with accession number KCTC 13057BP.

The present inventors introduced a mutation into the Streptomyces scopulididis KR-001 strain (Accession No. KCTC 12156BP) using a compound, and the content of herbicidin A and B, herbicidal active ingredients, was higher than that of the parent strain. High strains were selected and named Streptomyces scopuliridis N29 strains (hereinafter referred to as N29 strains).

The present inventors deposited the novel strain N29 at Korea Biotechnology Research Institute (KCTC) on June 28, 2016 and received accession number KCTC 13057BP.

In addition, the present invention provides a herbicide composition for weed control containing any one or more selected from the group consisting of Streptomyces scopulididis N29 strain deposited with Accession No. KCTC 13057BP, and cultures thereof.

The weed is preferably any one selected from the group consisting of ragweed weeds, grass and weeds, broadleaf weeds, and heating weeds.

The weeds in the ragweed are Ambrosia trifida L., ragweed ( Ambrosia artemisiifilia L.), Artemisia annua L., Artemisia capillaris L., Artemisia japonica L., And wormwood ( Artemisia pricceps Pamp.) And any one selected from the group consisting of, but not limited thereto.

The flower and the weed is preferably any one selected from the group consisting of dianthus ( Digitaria ciliaris ), sorghum ( Sorghum bicolor ), wheatgrass ( Elymus tsukushiensis var.transiens) and dolpi ( Echinochloa crus-galli ).

The broadleaf weed is preferably selected from the group consisting of Abutilon theophasti , Aeschynomeme indica , Abutilon avicennae , Xanthium strumarium , and Calystegia sepium var. Japonicum. .

The heat weed is a weed that is difficult to control, and any one selected from the group consisting of Sicyos angulatus, Humsulus japonicus, Artemisia princes, Equisetum arvense, and Clover Trifolium repens is preferable. One is not limited thereto.

The herbicide composition for weed control may include a carrier and / or a diluent. As used herein, the term 'carrier' refers to an inert, organic or inorganic, having an active ingredient mixed or made for the application, or storage, transport and / or handling thereof of a plant or other subject to be treated. Mean material. Examples of such diluents or carriers include, but are not limited to, water, milk, ethanol, mineral oil, glycerol.

The present inventors confirmed that the culture filtrate of the strain N29 by foliar treatment in the greenhouse grass in the greenhouse conditions for excellent killing power and the drug effect persists over time (see Table 3 to Table 5).

The present inventors confirmed that the culture filtrate of the N29 strain cultured in ragweed under the packaging conditions, the superior killing power and the drug efficacy continued over time (see Table 6 to Table 7).

Actinomycetes are known to produce useful secondary metabolites, examples of which are herbicidally active materials include herbimycin (Li et al., 2003) and bialaphos (Bayer, et al., 2004; Duke et al., 1996 ), albucidin (Hahn et al., 2009). Herbicidin A and B, which show strong herbicidal activity even in the culture filtrate of S. scopuliridis KR-001, the parent strain of the N29 strain of the present invention (Won et al., 2015). , F is known (Choi et al., 2012a). In comparison with the parent strain, N29, the strain of the present invention, had significantly higher content of herbicidin A and B in the culture filtrate than herbicidal A and B in the culture strain of the KR-001 strain, the parent strain (Table 2). Reference)

In addition, the present inventors compared the herbicidal activity of the culture filtrate of the culture filtrate of the strain N29 strain and the culture filtrate of the parent strain KR-001 strain and another strain M40 strain UV-mutated from the parent strain KR-001 strain, as a result It was confirmed that the culture filtrate of the strain N29 strain was significantly higher than the culture filtrate of the KR-001 strain and the culture filtrate of the M40 strain (see Fig. 6).

Therefore, the Streptomyces scopulididis N29 strain, or its culture deposited with accession number KCTC 13057BP of the present invention, weeds because we show excellent herbicidal power against weeds, grasses and weeds, broadleaf weeds, heating weeds in ragweed. It can be usefully used as an active ingredient of the herbicide composition for control.

In addition, the present invention comprises the step of treating any one or more selected from the group consisting of Streptomyces scopulididis N29 strain deposited with the accession number KCTC 13057BP, and its culture filtrate to weeds, seeds or habitats thereof. A weed control method is provided.

The treatment may be a foliage treatment applied directly to the stems and leaves of the plant, or a soil treatment applied to the soil, but is not limited thereto, and exhibits excellent herbicidal power against weeds, grass and weeds, broadleaf weeds, and heating weeds. Can be.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following Examples are intended to illustrate the present invention, and the content of the present invention is not limited by the Examples.

Example 1 Isolation of Strain N29

Streptomyces scopuliridis KR-001 strains were cultured in the media and conditions of Table 1 below.

Separation medium and composition pH Incubation temperature Incubation period HV agar (g / ℓ) and humic acid 1
Na2HPO4 0.5
KCl 1.71
MgSO47H2O 0.05
FeSO47H2O 0.01
CaCO3 0.02
Agar 18 pH 7.2 28 ℃ 7-30 days

The strain was improved by treating the compound with the Streptomyces scopulididis KR-001 strain (following NTG).

Specifically, Streptomyces scopulididis KR-001 strains were cultured in a Bennett agar plate medium at 27 ° C. for 15 days to induce sporulation, and by adding 30% (v / v) glycerol solution Spore suspension was prepared and stored in a -70 ℃ freezer. In order to prepare a mutant strain, NTG (N-methyl-N-nitro-N-nitrosoguanidine) solution was treated in a spore suspension at 100 ㎍ / mL for 3 hours, washed three times with sterile water and plated on a plate medium. It was.

Mutant colonies formed on the Bennet agar plate medium were shaken and cultured at 27 ° C. and 150 rpm for 7 days, respectively, after passage to Bennett broth, and then the herbicidal herbicidin A and B contents were increased. The highest N29 strain was finally selected relative to the strain. Selection strains were selected from CN9 medium (1% (w / v) soybean meal, 3% (w / v) glucose, 1% (w / v) soluble potato starch (potato starch) selected to improve herbicidine productivity. )) Inoculated in 100 mL and incubated at 27 ° C. and 160 rpm for 7 days, followed by centrifugation (8,000 rpm, 15 minutes) to remove the cells and the culture filtrate as a supernatant for analysis of herbicidin content and herbicidal activity. Used as a sample.

Example 2 Analysis of Herbicidin Content in N29 Strain Solution

N29 strain isolated in Example 1, Streptomyces scopulidis KR-001 strain and Streptomyces scopulididis M40 deposited with known strain Accession No. KCTC 12156BP (Streptomyces scopululi from Korea Research Institute of Chemical Research) After culturing the strain, J. Microbiol.Biotechnol. (2017), 27 (5), 947-955), which was prepared by irradiating UV to the Leadis KR-001 strain, was cultured in CN9 medium in the same manner as in Example 1. Purified using HPLC, Herbicidine A (purity 98.5%) and Herbicidin B (purity 98%) were dissolved in methanol solution and diluted sequentially, followed by analytical HPLC (elution solvent: 35% methanol, elution ratio: 1). ml / min, detection: 210, 254 nm) was performed and the area of the peaks at which the respective herbicidin A and B were eluted was calculated to prepare a quantitative graph. HPLC analysis of the culture filtrate of each strain was carried out under the above conditions to calculate the area of the herbicidin peak visible at 254 nm using a trend line graph and to estimate the content of the substance to analyze the content of the herbicidin in the culture filtrate. It was.

The content of Herbicidin A and B produced in the culture filtrate of the strain N29 strain, the culture filtrate of the parent strain KR-001 strain and the culture filtrate of the M40 strain is shown in Table 2 by HPLC analysis.

Strain Herbicidine content (µg / mL) Herbicidin A Herbicidin B N0029 639.4 211.1 M40 491.6 34.73 KR-001 112.8 16.6

As a result, as shown in Table 1, the culture filtrate of the N29 strain was 5.7 times (639.4 μg / ml) and 12.7 times (211.1 μg / ml) of herbicidin A and B, respectively, than the culture filtrate of the parent strain KR-001. Ml), and it was confirmed that they contained 1.3 and 6.1 times more herbicidin A and B than the culture filtrate of the other mutant strain N40 strain, respectively.

<Example 3> Evaluation of foliage treatment activity in greenhouse conditions

Example 3-1 cultivation of ragweed

The ragweed ( Ambrosia artemisiifolia) seeds were harvested around Mihocheon, Cheongju-si, Chungbuk, Korea in October-November 2017, and selected and used as faithful seeds when stored in a dry state at a low temperature of 6 ℃. When the seedlings of horticulture, stored in cold storage, are filled with 350 cm ² square plastic pots and the cotyledon emerges above the ground, the surface area is 40 cm ² polystyrene cups for the early and middle foliage. Dragons were implanted in plastic pots with a surface area of 314 cm ² and managed under greenhouse conditions (30/25 ± 5 ° C, Light / Dark = 14 / 10h). After transplantation, when the growth period was different by test slap, a uniform individual was selected and used.

Example 3-2 Evaluation of Swine Grass Foliage Treatment Activity in Greenhouse Conditions

The herbicidal foliage treatment herbicidal activity of the strain cultured N29 strain prepared in Example 2 was evaluated by the following method.

When the growth time of the ragweed growing in the greenhouse after transplanting to the pot as in Example 3-1 became early (2-3 leaves), middle (4-5 leaves), late (7-8 leaves), Example The N29 strain culture filtrate prepared by the method of 2 was treated by dilution (Tween-20®, 0.1% (v / v)). The treatment concentration for each growth period was the initial and middle treatment of the culture filtrate, the supernatant from which the cells were removed from the culture filtrate prepared in Example 2, and 2, 4, 8-fold dilutions, and in the case of late treatment, the culture filtrate and 2 The dilutions were folds and quadruple dilutions, where the amount of treatment was 3 ml per pot for initial treatment, 5 ml for mid-term treatment, and 20 ml for late treatment. After treatment, the same greenhouse conditions were administered, and after 5, 10, and 15 days of treatment, external symptoms and efficacy were examined according to the criteria (0; no effect, 100; complete test).

<Example 3-3> Results of initial ragweed foliage treatment activity evaluation in greenhouse conditions

As a result of evaluating initial ragweed foliage treatment activity in greenhouse conditions in the same manner as in Example 3-2, it was confirmed that the treatment of N29 strain culture filtrate in ragweed of 2-3 leaves under greenhouse conditions showed a very good control effect. . After 5 days of treatment, the control effect of ragweed was 100% in the culture filtrate stock and 2-fold, 4-fold and 8-fold dilutions. . In 8-fold dilutions, new leaves developed at the growth point after 5 days of treatment, after which the regenerated new leaves no longer settled and continued. The herbicidal properties of N29 strain culture filtrates on ragweed were very fast action, and the drug duration was maintained for a long time. In other words, when the culture solution was treated with ragweed, external herbicidal symptoms began to be expressed within 24 hours, and completely died within 3 days, and this effect persisted after 3 weeks of treatment. The main symptom in appearance was chlorosis at the beginning of treatment, followed by leaf burn-down over time, and ultimately defoliation.

Dilution factor Culture filtrate herbicidal activity (%) ^y 5DAT ^z 10DAT 15 DAT x1 100 100 100 x1 / 2 100 100 100 x1 / 4 100 100 100 x1 / 8 100 98 98

^y Culture filtrate herbicidal activity was determined by pedagogical irradiation (0; no effect, 100; complete test). ^z DAT: days after treatment

<Example 3-4> Evaluation results of medium-term ragweed grass foliage treatment activity in greenhouse conditions

In the same manner as in Example 3-2, when the N29 strain culture filtrate was treated in GHG at the 4 to 5 leaf stages, the control effect after 15 days of treatment was 2 times, 4 times, and 8 times the culture filtrate. The dilutions were very good at 100, 100, 98 and 98%, respectively (Table 4, Figure 2). After 5 days of treatment, the control effect at the same concentration was 90% in all treatment concentrations, but the efficacy increased as the days of treatment increased, and after 10 days of treatment, they were 98, 98, 98, 95%, respectively. It showed high control effect of more than 98% in the treatment concentration range. After 15 days of treatment, it was completely controlled at concentrations higher than 2-fold dilutions, and at the concentration lower than 4-fold dilutions, very small new leaves were developed at the growth point as in the 8-fold dilutions at the initial treatment. In the mid-term treatment, the same herbicidal properties and external symptoms were expressed as in the initial treatment, and even after 3 weeks of treatment, no new leaf growth occurred even at concentrations below the 4-fold dilution where new leaves were formed. In the mid-term treatment, it was judged to be an effective control measure.

Dilution factor Culture filtrate herbicidal activity (%) ^y 5DAT ^z 10DAT 15 DAT x1 90 98 100 x1 / 2 90 98 100 x1 / 4 90 98 98 x1 / 8 90 95 98

^y Culture filtrate herbicidal activity was determined by pedagogical irradiation (0; no effect, 100; complete test). ^z DAT: days after treatment

Example 3-5 Late Swine Foliage Treatment Activity Evaluation Results Under Greenhouse Conditions

The ragweed begins to germinate in its natural state after the first spring rains, and blooms and fruites from summer to autumn (Kim and Park, 2009). After a certain period of early growth, growth is so fast that the height and number of nodes grow exponentially. Therefore, the growth is very active after the mid-stage season, if you do not control the ragweed at this time, further control becomes more difficult. In greenhouse conditions, control of ragweed after 7 leaves can be an indicator of the possibility of control in the natural state.

When the N29 strain culture filtrate was treated with ragweed 7-8 leaves in the same manner as in Example 3-2, it was completely controlled in the pretreatment concentration range (Table 5, Figure 3). After 5 days of treatment, the control effect of ragweed was 98, 90 and 90% in the culture stock and 2-fold and 4-fold dilutions, respectively. The control effect was shown, and even after the treatment was continued without the regenerated individual, 15 days after the treatment showed a 100% control effect in the concentration range. As described above, the N29 strain culture filtrate could be expected to be able to control the ragweed generated in the natural state because it can effectively control the ragweed in the seven leaf stage in the greenhouse conditions.

Dilution factor Culture filtrate herbicidal activity (%) ^y 5DAT ^z 10DAT 15 DAT x1 98 100 100 x1 / 2 90 100 100 x1 / 4 90 100 100

^y Culture filtrate herbicidal activity was determined by pedagogical irradiation (0; no effect, 100; complete test). ^z DAT: days after treatment

Example 4 Evaluation of Competitiveness in the Packaging Conditions

The field test site was conducted by selecting a flat colony where pig grasses were uniformly inhabited at 510-14 Gagok-ri, Ochang-eup, Cheongwon-gun, Cheongju-si, Chungcheongbuk-do, and the test period was from mid-May 2018 to late June. The treatment compartment was 1 x 1 m (1 m ² ), and the treatment period was early (3-4 leaves) and mid-term (5-6 leaves). The initial length of ragweed was 108.6 mm in the middle stage. Was 171.4 mm on average. Treatment concentrations were culture stock and 2-fold and 4-fold dilutions, and the treatment volume was 200 ml / 1 m ² for each treatment concentration (Tween-20 ^® , 0.1% (v / v)) for both initial and mid-term treatments. Spray leaf treatment was performed using a hand spray (nano electric sprayer, BIO CHEM KOREA Co., LTD., Bucheon, Korea). After 7, 14 and 21 days of treatment, the external symptoms and efficacy were examined according to the reference table (0; no effect, 100; complete test).

N29 strain culture filtrate showed a very good control effect on ragweed generated in the natural state (Table 6, Figure 4). When the N29 strain culture filtrate was treated in 3-4 leaf ragweed under the packing condition, the control effect of the culture stock and 2-fold and 4-fold dilutions were 99, 99 and 70% after 7 days, respectively. The herbicidal power increased more and more, and after 21 days of treatment, the control effect was 100% in the total concentration range. Especially, in the case of 4-fold dilution, the efficacy was improved to 70% after 5 days of treatment, and to 95% after 14 days of treatment, and new leaves were not completely regenerated at the growth point after 21 days of treatment.

Dilution factor Culture filtrate herbicidal activity (%) ^y 7DAT ^z 14DAT 21DAT x1 99 100 100 x1 / 2 99 99 100 x1 / 4 70 95 100

^y Culture filtrate herbicidal activity was determined by pedagogical irradiation (0; no effect, 100; complete test). ^z DAT: days after treatment

In addition, the treatment of N29 strain culture filtrate in 5-6 leaf ragweed showed a very good control effect (Table 7, Fig. 5), 2 times, 4 times the culture stock solution and 7 days and 14 days after treatment The control effects in the dilutions were all 100% and 99, 99 and 98% after 21 days of treatment, respectively. After 21 days of treatment, most of the ragweeds in the treatment compartments were completely controlled, showing signs of very fine regeneration of the new leaves near the growth point of some individuals that were expected to have a strong growth at the time of treatment.

Dilution factor Culture filtrate herbicidal activity (%) ^y 7DAT ^z 14DAT 21DAT x1 100 100 99 x1 / 2 100 100 99 x1 / 4 100 100 98

^y Culture filtrate herbicidal activity was determined by pedagogical irradiation (0; no effect, 100; complete test). ^z DAT: days after treatment

Soil actinomycetes N29 strain culture filtrate from the results of the experiment is not only excellent in the control effect for ragweed in the greenhouse conditions, but also effectively control the ragweed occurring in the natural state, so it is a candidate for natural plant-based ragweed control agent It is expected to be useful as a material. However, in the case of ragweed that occurs in the natural state, the possibility of regeneration may not be excluded depending on the growth situation as shown in Table 7 and Figure 5 after mid-term. Judging.

Example 5 Comparison of herbicidal activity between N29 strain, KR-001 strain and N40 strain

In order to compare the herbicidal activity of the known M40 strains in which the N29 strain and the parent strain KR-001 strain (Accession No. KCTC 12156BP) and the parent strain KR-001 strain were UV-mutated, experiments were carried out in the same manner as in Example 3-2. When the growth period of the ragweed is late (7-8 leaves), 5 times the culture filtrate of N29 strain culture filtrate , KR-001 strain culture filtrate, or M40 strain prepared in the same manner as in Example 2 After dilution, the treatment was carried out in the same greenhouse conditions, and after 5, 10 and 15 days of treatment, the external symptoms and efficacy were examined according to the reference table (0; no effect, 100; complete test).

As a result, as shown in Figure 6, when the 5-fold dilution of the N29 strain culture filtrate was treated to the ragweed, when treated with a 5-fold dilution of the KR-001 culture filtrate of the parent strain and a 5-fold dilution of the M40 strain culture filtrate It was confirmed that the herbicidal activity was significantly higher than when compared.

Korea Research Institute of Bioscience and Biotechnology KCTC13057BP 20160630

Claims (9)

Streptomyces scopuliridis N29 strain deposited with accession number KCTC 13057BP.
A herbicide composition for weed control comprising any one or more selected from the group consisting of Streptomyces scopulidis N29 strain deposited with accession number KCTC 13057BP, and a culture filtrate thereof.
The weed control herbicide composition according to claim 2, wherein the weeds are ragweed weeds, flower weeds, broadleaf weeds or heating weeds.
The herbicide composition for weed control according to claim 3, wherein the weed in the ragweed is any one selected from the group consisting of maple leaf ragweed ( Ambrosia trifida L.) and ragweed ( Ambrosia artemisiifilia L.).
4. The method of claim 3 wherein the hwabongwa weeds, characterized in that at least one selected from the group consisting of wire grass (Digitaria ciliaris), sorghum (Sorghum bicolor), couch grass (Elymus tsukushiensis var. Transiens) and dolpi (Echinochloa crus-galli) Herbicide composition for weed control.
4. The method of claim 3 wherein the broadleaf weeds is composed of any one selected from the group consisting of Solanum nigrum (Abutilon theophasti), Aeschynomene indica (Aeschynomeme indica), Abutilon theophrasti (Abutilon avicennae), Xanthium strumarium (Xanthium strumarium) and bindweed (Calystegia sepium var. Japonicum) Herbicide composition for weed control, characterized in that.
The method of claim 3, wherein the heating weed is any one selected from the group consisting of Sicyos angulatus , Humulus japonicus , Artemisia princeps , Equisetum arvense and Clover Trifolium repens Herbicide composition for weed control, characterized in that.
Weeds, their seeds or their habitats, comprising the step of treating at least one selected from the group consisting of Streptomyces scopulididis N29 strain deposited with Accession No. KCTC 13057BP, and its culture filtrate; Control method.
9. The weed control method according to claim 8, wherein the weeds are ragweed weeds, flower weeds, broadleaf weeds or heat weeds.

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