KR102066384B1 - A cosmetic composition having excellent skin barrier improvement with filaggrin activity - Google Patents

Abstract

The present invention relates to a cosmetic composition excellent in skin barrier improvement and filaggrin activity. The cosmetic composition includes ceramides, cholesterol, fatty acids, an acacia pollen extract and unsaponifiable materials of vegetable oil.

Description

Cosmetic composition with excellent skin barrier improvement and filaggrin activity {A COSMETIC COMPOSITION HAVING EXCELLENT SKIN BARRIER IMPROVEMENT WITH FILAGGRIN ACTIVITY}

The present invention relates to a cosmetic composition excellent in skin barrier improvement and filaggrin activity.

The skin is an organ that exists on the outside of the body and has a unique structure that contains various cells.It protects the body from external stimuli such as preventing evaporation of moisture in the body, controlling temperature, and ultraviolet rays, and harmful substances (microorganisms and pollutants). It acts as a primary shield of the human body, such as prevention of infiltration.

The skin is composed of several layers, the outermost of which is called the epidermis, and is divided into subdivided layers of dermis and subcutaneous fat. The double epidermis contains the stratum corneum and acts as a skin barrier to protect the human body.

In the epidermis, while the basal cells proliferating in the basal layer move to the stratum corneum, keratinocytes are formed in a stepwise change in form and function. This process is called keratinization of keratinocytes. The keratinocytes present in the stratum corneum are eliminated from the skin after a certain period of time, and new keratinocytes from the basal epidermis replace the function. In keratinization, keratinocytes produce natural moisturizing factors and intercellular lipids.

Natural moisturizing factors in the stratum corneum play an important role in maintaining the stratum corneum of the stratum corneum properly hydrated. On the other hand, profilaggrin present in the granular layer of the epidermis changes to filaggrin during the final differentiation of keratinocytes, and the filaggrin is decomposed into a natural moisturizing factor as it rises to the top of the keratinous layer. The natural moisturizing factors generated by the decomposition of filaggrin are powerful moisturizers that combine with moisture to maintain the moisture of the stratum corneum and to control the pH of the stratum corneum to strengthen the stratum corneum, and the filaggrin is the final differentiation process of keratinocytes When the keratinocyte membrane is formed in the keratinocytes make a solid and flat structure to act as a brick of the skin barrier.

The problem of filaggrin expression causes not only the abnormal structure of keratinocytes and the deterioration of skin barrier function, but also the decrease of the natural moisturizing factor of the stratum corneum, the water content in the stratum corneum decreases and the stratum corneum pH rises. This activates the serine protease, which destroys the corneodesmosome. This causes the stratum corneum to drop off prematurely and cause dry skin.

Promoting the expression of filaggrin in the stratum corneum increases the natural moisturizing factor through the hydrolysis of filaggrin, thereby enhancing skin barrier function, and increasing the density of corneocytes to achieve a firm skin barrier. .

On the other hand, the intercellular lipid in the stratum corneum is composed of 50% ceramide, 25% cholesterol, 15% free fatty acid, and fills the space between the keratinocytes to give the binding force between the keratinocytes to form a keratinocyte and lamellar structure. The stratum corneum formed with the lamellar structure prevents the leakage of natural moisturizing factors and substances in the body to provide an environment for normal biochemical metabolism of the epidermis and to prevent external contaminants from invading the skin. If the balance of components or contents of the intercellular lipids of the stratum corneum is broken, the lamellar structure of the keratinocytes and the intercellular lipids is not maintained, causing natural moisturizing factor and water loss, causing dryness of the skin and impaired barrier function.

Between the epidermis and the dermis, there is a dermal epidermal junction consisting of a basal membrane. The basal membrane of the epidermis-dermis junction has a three-layer structure of a transparent plate, a dense plate and a base plate.

The most important function of the epidermal-dermal junction is to connect the epidermis and the dermis by bonding. Epidermal-dermal junctions serve as a support for epidermal cells by the junction of the epidermis and the dermis, as well as the selective transmission and transport of substances between the epidermis and the dermis, and by controlling the differentiation of adjacent skin cells to regulate the proliferation of epidermal cells. do. If the epidermal-dermal junction is damaged, the selective permeation and filtration of the substance may not be possible, thereby increasing the likelihood of contaminants penetrating to the dermis, and the structure of the skin may not be maintained normally, resulting in abnormal function of the entire skin barrier.

Background art related to the present invention is disclosed in Korean Unexamined Patent Publication No. 2018-0027375 (published on March 14, 2018, the name of the invention: cosmetic composition for moisturizing the skin comprising a plant Taiji component).

An object of the present invention is to provide a cosmetic composition excellent in skin barrier improvement and filaggrin activity.

Another object of the present invention is to provide a cosmetic composition that prevents skin irritation, has excellent formulation stability, and is harmless to the human body.

Still another object of the present invention is to provide a cosmetic composition having excellent skin moisturizing and atopic improvement effects.

One aspect of the present invention relates to a cosmetic composition excellent in skin barrier improvement and filaggrin activity. In one embodiment of the cosmetic composition is ceramide (ceramide), cholesterol (cholesterol), fatty acids, acacia (Acacia), pollen (pollen) extract and vegetable oils bulgeom includes a cargo, wherein the vegetable oil bulgeom cargo dolkong five days bulgeom cargo (glycine soja oil unsaponifiables, olea europaea oil unsaponifiables, and wheat germ oil unsaturated triticum vulgare germ unsaponifiables).

In one embodiment, 0.1 to 7% by weight of ceramide, 0.01 to 5% by weight of cholesterol, 0.01 to 5% by weight of fatty acid, 0.001 to 1% by weight of acacia pollen (flower pot) extract and 0.01 to 1% vegetable oil non-compensation based on the total weight of the cosmetic composition 10 wt% may be included.

In one embodiment, the vegetable oil non-compensation may include 1: 1 soybean oil non-compensation, olive oil non-compensation, and wheat germ oil non-compensation in a weight ratio of 1: 1-3: 0.5-2.

In one embodiment, the fatty acid, ceramide and vegetable oil non-compound may be included in a weight ratio of 1: 1-7: 0.5-15.

In one embodiment the ceramide may include one or more of ceramide 1, ceramide 2, ceramide 3, ceramide 4, ceramide 5 and ceramide 6.

In one embodiment, the fatty acid may include one or more of palmitic acid, stearic acid, oleic acid, and linolenic acid.

In one embodiment the cosmetic composition may be a solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray formulation. .

In one embodiment the cosmetic composition may be Oil-in-Water (O / W), Water-in-Oil (W / O) or Water-in-Silicone (W / S) formulation.

In one embodiment, the cosmetic composition may be excellent in skin barrier improvement, skin moisturizing and atopic improvement effect due to the filaggrin activity and lipid content.

The cosmetic composition according to the present invention prevents skin irritation, has excellent formulation stability, is harmless to the human body, and has an excellent skin barrier and filaggrin activity effect, and may have an excellent skin moisturizing and atopy improving effect.

1 is a graph showing a change in the amount of the epidermal moisture loss of the application site to which the cosmetic composition according to the present invention is applied and the uncoated area to which the embodiment is not applied.
Figure 2 is a graph comparing the change in the amount of hard epidermal moisture loss between the application site to which the cosmetic composition according to the present invention is applied, and the uncoated site to which the embodiment is not applied.
Figure 3 is an optical micrograph showing the application site and the uncoated site of Example 1 of the test subjects of the transdermal moisture loss of the present invention.
Figure 4 is a graph showing the results of evaluating the 100-hour moisturizing persistence between the application group to which the cosmetic composition is applied according to the present invention, the group to which the above example was applied but the peeling group and the non-coating group to which the example was not applied. to be.
Figure 5 is a graph comparing the lasting 100 minutes moisturizing duration between the application group to which the cosmetic composition is applied according to the present invention, the group to which the above example was applied but the peeling group and the non-coating group to which the above example was not applied.
Figure 6 is a photograph showing the skin barrier improvement effect of the application site and the uncoated site of Example 1 of the present invention.

In the following description of the present invention, when it is determined that detailed descriptions of related well-known technologies or configurations may unnecessarily obscure the subject matter of the present invention, the detailed description will be omitted.

The terms to be described below are terms defined in consideration of functions in the present invention, and may be changed according to intentions or customs of users or operators, and the definitions should be made based on the contents throughout the specification for describing the present invention.

Cosmetics  Composition

One aspect of the invention relates to cosmetic compositions. In one embodiment of the cosmetic composition is ceramide (ceramide), cholesterol (cholesterol), fatty acids, acacia (Acacia), pollen (pollen) extract and vegetable oils bulgeom includes a cargo, wherein the vegetable oil bulgeom cargo dolkong five days bulgeom cargo (glycine soja oil unsaponifiables, olive oil ( Olea) europaea oil unsaponifiables), and wheat germ oil bulgeom cargo (triticum vulgare germ unsaponifiables).

Hereinafter, the components of the cosmetic composition will be described in more detail.

Ceramide

The ceramide is a component present in the stratum corneum of the skin epidermal layer and occupies about 40-50% of the lipid component between keratinocytes. The ceramide forms a multiple lamellar structure between keratinocytes with other keratinocyte lipid components, thereby inhibiting water evaporation from the skin and maintaining moisture to soften the skin and keep the skin healthy. In addition, the ceramide has a hydrophilic group and a lipophilic group at the same time can suppress the evaporation of water in the skin, and acts as a skin barrier to block the penetration of foreign substances in the skin can play an important function in the control of inflammation or wound recovery.

In one embodiment the ceramide may comprise one or more of ceramide 1, ceramide 2, ceramide 3 (or ceramide NP), ceramide 4, ceramide 5 and ceramide 6.

In one embodiment the ceramide may be included 0.1 to 7% by weight based on the total weight of the cosmetic composition. When included in the content, the skin barrier improvement and moisturizing effect can be excellent. For example, 0.1 to 5% by weight may be included.

cholesterol

The cholesterol (cholesterol) may have an excellent skin protective film forming effect when applied to the skin.

In one embodiment the cholesterol may be contained 0.01 to 5% by weight based on the total weight of the cosmetic composition. When included in the content of the formulation stability, and easy to form a skin protective film, the skin barrier improvement effect can be excellent. For example, 0.01 to 3% by weight may be included.

fatty acid

The fatty acid may include one or more of palmitic acid, stearic acid, oleic acid, and linolenic acid. When applying the fatty acid, it may be excellent in the moisturizing effect and skin barrier improvement effect. For example, the fatty acid may comprise one or more free fatty acids of palmitic acid and stearic acid.

In one embodiment, the fatty acid may be contained 0.01 to 5% by weight based on the total weight of the cosmetic composition. When included in the above range, the formulation stability and moisturizing effect may be excellent at the same time. For example, 0.01 to 3% by weight may be included.

Acacia Pollen Extract

The acacia pollen (pollen) extract is an extract derived from pollen of Acacia ( Acacia ), and enhances the skin barrier strengthening and moisturizing effect of the present invention. In addition, the acacia pollen (pollen) extract contains a large amount of useful components, such as proteins, vitamins and minerals.

In one embodiment the acacia pollen (pollen) extract can be obtained by applying the extraction method of the general method used in the art. For example, it can be obtained using one or more methods of ultrasonic extraction, hydrothermal extraction, fermentation extraction and solvent extraction. For example, the solvent extraction may be obtained by adding 10 to 1000 parts by weight of the extraction solvent to 100 parts by weight of acacia pollen, and extracting at 40 to 90 ° C. The extraction solvent may be water, acetone, ethyl acetate, butyl acetate, diethyl ether, benzene, chloroform, hexane and alcohol having 1 to 10 carbon atoms. These can be used individually or in mixture of 2 or more types. In one embodiment, the alcohol having 1 to 10 carbon atoms includes lower monohydric alcohols such as methanol, ethanol, butanol, pentanol, and hexanol, 1,2-pentanediol, 1,5-pentanediol, hexanediol, and heptanediol. And lower trihydric alcohols such as intermediate dihydric alcohols such as octanediol and decanediol, propylene glycol, 1,3-butylene glycol, and glycerin may be used. These can be used individually or in mixture of 2 or more types.

In one embodiment, the acacia pollen (pollen) extract may be included in an amount of 0.001 to 1% by weight based on the total weight of the cosmetic composition. When included in the above range may be excellent formulation stability, skin barrier improvement and moisturizing effect. For example, it may be included in 0.001 to 0.5% by weight.

vegetable oil Unchecked cargo

The vegetable oil non-compound has an excellent pilaggrin activity and is included to improve skin conditioning and skin barrier improvement. In one embodiment, the vegetable oil non-compound is a glycine glycoside ( glycine) soja oil unsaponifiables, olive oil ( Olea) europaea oil unsaponifiables), and wheat germ oil bulgeom cargo (triticum vulgare germ unsaponifiables).

In one embodiment, the sorghum oil non- solvate is a non-saponified soybean oil fraction component in the process of refining and recovering soybean fatty acid, to impart skin conditioning. In one embodiment, the dolkong oil non- solvate may be contained 0.01 to 4% by weight based on the total weight of the cosmetic composition. When included in the above range, while having excellent formulation stability, the skin barrier improvement effect may be excellent. For example, 0.01 to 2% by weight may be included.

In one embodiment, the olive oil non-compensation is a non-gumified olive oil fraction in the process of purifying and recovering the olive oil fatty acid, imparts conditioning to the skin and hair, invigorates and softens the skin, and gives a soft skin. And hair care. In one embodiment, the olive oil non-compound may be included in an amount of 0.01 to 4% by weight based on the total weight of the cosmetic composition. When included in the above range, while having excellent formulation stability, the skin barrier improvement effect may be excellent. For example, 0.01 to 1.5% by weight may be included.

In one embodiment, the wheat germ oil non-compensation imparts skin conditioning with an unbranched wheat germ oil fraction component during the purification and recovery of wheat germ oil fatty acids. In one embodiment, the wheat germ oil non-compound may be included in an amount of 0.01 to 4% by weight based on the total weight of the cosmetic composition. When included in the above range, while having excellent formulation stability, the skin barrier improvement effect may be excellent. For example, 0.01 to 1.5% by weight may be included.

In one embodiment, the vegetable oil non-compound may be included in an amount of 0.01 to 10 wt% based on the total weight of the cosmetic composition. When included in the above range, while having excellent formulation stability, the skin barrier improvement effect may be excellent. For example, 0.05 to 5% by weight may be included.

In one embodiment, the dol bean oil unchecked, olive oil unchecked and wheat germ oil unchecked may be included in a weight ratio of 1: 1-3: 0.5-2. When included in the weight ratio may be excellent synergistic effect between the vegetable oil non-gum component constituents excellent formulation stability, excellent skin barrier improvement effect.

In one embodiment, the fatty acid, ceramide and vegetable oil non-compound may be included in a weight ratio of 1: 1-7: 0.5-15. When included in the weight ratio, the synergistic effect between the vegetable oil non-compensated constituents is excellent, while the formulation stability is excellent, the skin protective film formation effect, moisturizing effect and skin barrier improvement effect may be excellent at the same time. For example, it may be included in a weight ratio of 1: 1-7: 1-14.

The cosmetic composition of the present invention may be prepared in a formulation commonly prepared in the art. In one embodiment, the cosmetic composition comprises a solution, suspension, emulsion, essence, paste, gel, cream, lotion, body cream, body lotion, powder, soap, surfactant-containing cleansing, cleansing water, oil, powder foundation, Emulsion foundations, wax foundations or spray formulations.

In one embodiment the cosmetic composition of the present invention is Oil-in-Water (O / W), Water-in-Oil (W / O), Water-in-Silicone (W / S), Oil-in-Water-in -Oil (O / W / O) or Water-in-Oil-in-Water (W / O / W) formulations.

When the formulation of the present invention is a paste, cream or gel, animal oils, vegetable oils, waxes, paraffins, starches, trachants, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicas, talc or zinc oxide may be used as carrier components. have.

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used, in particular in the case of a spray, additionally chlorofluorohydrocarbon, propane / Propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, solvents, solubilizers or emulsions are used as carrier components, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1, Fatty acid esters of 3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, liquid carrier diluents such as water, ethanol or propylene glycol, suspending agents such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxy, bentonite, agar or tracant and the like can be used.

Aliphatic alcohol sulfates, aliphatic alcohol ethersulfates, sulfosuccinic acid monoesters, isethionates, imidazolinium derivatives, methyltaurates, sarcosinates, fatty acid amide ethers as carrier components when the formulation of the present invention is a surfactant-containing cleansing Sulfates, alkylamidobetaines, aliphatic alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives or ethoxylated glycerol fatty acid esters and the like can be used.

For example, the cosmetic composition of the present invention is polysorbate 60, sorbitan sesquioleate, stearic acid, cetyl alcohol, squalane, glycerin, butylene glycol, arginine, carbomer, tocopheryl acetate, propanediol, 1, It may further comprise one or more of 2-hexanediol and purified water.

In addition, the cosmetic composition may include a surfactant, a moisturizer, a plant oil, an ester oil, a sunscreen, an antioxidant, a pH adjuster, a pigment, a perfume, a preservative, a thickener, an emulsifier, a solubilizer, a plant extract, and the like.

The cosmetic composition according to the present invention prevents skin irritation, has excellent formulation stability, is harmless to the human body, and has an excellent skin barrier and filaggrin activity effect, and may have an excellent skin moisturizing and atopy improving effect.

Hereinafter, the configuration and operation of the present invention through the preferred embodiment of the present invention will be described in more detail. However, the following examples are provided to help the understanding of the present invention, and the scope of the present invention is not limited to the following examples. Details that are not described herein will be omitted since those skilled in the art can sufficiently infer technically.

Example  1-4 and Comparative example  1-6

To apply the ingredients and contents shown in Table 1 and Table 2, to prepare a cosmetic composition of the cream formulation in a conventional manner. Specifically, the Acacia pollen (Pollen) extracts of Tables 1 and 2 were prepared by adding 100 parts by weight of acacia pollen to 1000 parts by weight of ethanol, and heating and filtering the filtrate after concentration at 60 to 80 ° C. and freeze-drying. The vegetable oil non-compound (A) is a glyco-oil non-compensated glycine soja oil unsaponifiables), olive oil and olive oil bulgeom bulgeom cargo freight (olea europaea oil unsaponifiables and wheat germ oil ( triticum) vulgare germ unsaponifiables) containing 1: 1 to 3: 1 to 3 by weight, and the vegetable oil non-compound (B) is a 1: 1 to 3 weight ratio of olive oil non-soybean oil and olive oil non-sulfide olive oil The containing was used.

Test Example  1: Evaluate the effect of skin moisturizing and skin barrier improvement

In order to measure skin moisturization and barrier improvement when using the cosmetic compositions of Examples 1 to 4 and Comparative Examples 1 to 6, a total of 10 women, 20 to 55 years old, were selected for 10 groups, and 1 week for each product. After the use, the moisturizing effect and skin barrier improvement are shown in Table 3 below. The lowest score was set to 1, the highest score was set to 5, and the test was conducted, and the average value of the evaluation results was obtained.

Referring to the results of Table 3, when using Examples 1 to 4 it was found that the moisturizing effect and skin barrier improvement effect is superior to Comparative Examples 1 to 6.

Test Example  2: skin irritation evaluation

In the case of using the cosmetic compositions of Examples 1 to 4 and Comparative Examples 1 to 6, a skin patch test was conducted to investigate the skin irritation test. The subjects were selected as a person without skin disease at the test site of 20 ~ 45 years old. After wiping the upper arm part of the patch area with 70% ethanol and drying, 0.1 g of the above Example and Comparative Example cosmetic composition was added to the patch for 24 hours, and then the patch was removed and the skin reaction was examined within 1 hour, and the next day ( 48 hours after the start of the patch), it was examined again. The skin reaction was judged by the following test criteria. The skin patch test results are shown in Table 4 below.

* Skin test criteria

-No response ± weak positive reaction (erythema) + positive reaction (erythema) ++ strong positive reaction (erythema, edema) +++ severe positive reaction (erythema, edema, blister)

Referring to the results of Table 4, in the case of the cosmetic composition of Examples 1 to 4 of the present invention, it can be seen that there is almost no skin irritation compared to Comparative Examples 1 to 6.

Test Example  3: Formulation stability evaluation

In order to measure the stability to the degree of separation and discoloration for the cosmetics of Examples 1 to 4 and Comparative Examples 1 to 6, the refrigerator and daylight conditions are constantly maintained in a constant temperature bath 4 ℃ maintained at 45 ℃ and 25 ℃ Example and Comparative Example The composition was stored in a sample container and stored for 4 weeks to compare and measure the degree of separation and discoloration, and the results are shown in Table 5 below. At this time, the degree of separation and discoloration of the product was evaluated by dividing into six grades.

* Separation and discoloration evaluation criteria

0: no change 1: very little separation (discoloration) 2: little separation (discoloration) 3: little separation (discoloration) 4: severe separation (discoloration) 5: very severe separation (discoloration)

Referring to the results of Table 5, Examples 1 to 4 of the present invention, the separation and discoloration is less than the Comparative Examples 1 to 6 out of the configuration of the present invention was found to be excellent formulation stability.

Experimental Example  4: Hard cuticle  Moisture Loss Measurement

Transepidermal water loss (TEWL) was measured for Example 1 and Comparative Example cosmetic compositions, typically in Example 1.

Specifically, experiments were conducted on 22 female clinical teams. The skin conditions of 22 subjects who participated in the evaluation test were 4 dry skin, 14 moderate skin, 1 neutral skin, 2 middle skin and 1 oily skin, skin disease, itching, itching, erythema and cosmetic side effects. None of the subjects had any experience with adverse drug reactions, drug side effects, photosensitivity, or atopic disease.

The epidermal moisture loss amount was measured by using a measuring device (Vapometer) on the coated portion and the uncoated portion coated with the cosmetic composition of Example 1, and the results are shown in Table 6, FIGS. 1 and 2. Shown in

1 is a graph showing a change in the transdermal moisture loss amount of the coating site to which the cosmetic composition of Example 1 according to the present invention is applied and the uncoated area to which the above example is not applied, and FIG. It is a graph which compares the change of the amount of transdermal moisture loss between the application site | part which apply | coated the cosmetic composition and the uncoated site | part which did not apply the said Example.

In addition, FIG. 3 is an optical microscope photograph showing the application site and the uncoated site of Example 1 of the hard epidermal moisture loss test subject. Referring to Table 6 and the results of FIGS. 1 to 3, it was found that the application group to which the cosmetic composition of Example 1 was applied has a smaller amount of hard epidermal moisture loss than the non-coating group.

Experimental Example 5: 100 hours  Moisturizing Sustainability Assessment

About the said Example and the comparative example cosmetic composition, the moisturizing sustainability for 100 hours was evaluated about Example 1 typically. Twenty-two female clinicians were included in one group. Specifically, Example 1 was applied to the coating group, and Example 1 was applied, but the test product was measured by applying a coating amount of 20 μl / cm 2 to the test site of the forearm (width x length 3x3 cm). Divided into a group to which the peeling was applied and a non-coating group, and measured the 100 minutes moisturizing persistence using a Corneometer, the results are shown in Table 7, 4 and 5 below.

Figure 4 is a result of evaluating the 100-hour moisturizing persistence between the application group to apply the cosmetic composition of Example 1 according to the present invention, the application of Example 1 but the peeling group and the non-coating group not applied to Example 1 5 is a graph showing a coating group to which the cosmetic composition of Example 1 according to the present invention is applied, 100 minutes of moisturizing between the group to which Example 1 is applied but the peeling group is not applied, and Example 1 to which no coating group is applied. This is a graph comparing sustainability.

Referring to Table 7, FIG. 4 and FIG. 5, it was found that when applying Example 1 according to the present invention, the moisturizing sustainability was excellent.

Experimental Example  6: Skin barrier  Effectiveness evaluation

Twenty-two female clinicians applied the cosmetic composition of Example 1 once a day to a group for one week, and then asked about the questionnaire values, standard deviations, and answers in a survey conducted on skin barrier improvement effects. The percentages of 22 subjects were obtained and the results are shown in Table 8 below.

Referring to the results of Table 8, when applying Example 1 of the present invention, 95.5% of the test subjects were evaluated as good or better, it was found that the skin barrier improvement effect is excellent.

Experimental Example  7: Skin barrier  Improvement evaluation

Twenty-two female clinicians evaluated skin barrier improvement effects. Skin barrier damage was caused by using a 1% aqueous solution of sodium lauryl sulfate (SLS), and after 1 day of injury, the cosmetic composition of Example 1 was applied to the upper arm of the arm. It was applied once a day for 14 days to evaluate the skin barrier improvement effect.

6 is a photograph showing the skin barrier improvement effect of the application site and the uncoated site of Example 1 of the present invention. Referring to FIG. 6, it can be seen that the application of Example 1 of the present invention has an excellent skin barrier improvement effect.

Experimental Example  8: Pill-green  Expression increase effect confirmed

In order to confirm the effect of increasing the filaggrin expression of the cosmetic composition of the present invention, after adjusting the keratinocytes (HaCaT) to 5X10 ⁵ cells / well using DMEM medium with 10% FBS, inoculated in 12 well plate 18 Incubated for hours. After incubation, the samples were treated with vegetable oil non-compounds and acacia pollen (flower pot) extract components corresponding to Example 1 of the present invention according to the treatment concentrations of Table 9, and incubated for 24 hours. RNA was extracted from the cultured cells and the expression rate of filaggrin was confirmed through reverse transcription polymerase chain reation (RT-PCR). Filaggrin expression increase rate was calculated by the following formula, the results are shown in Table 9 below. Hyaluronic acid was used as a control.

Formula expression rate (%) = sample filaggrin expression / control filaggrin expression x 100

Referring to the results of Table 9, it was found that the application of the vegetable oil non-gummul and acacia pollen (pollen) extract components of Example 1 of the present invention, the filaggrin expression effect is excellent.

Simple modifications or changes of the present invention can be easily carried out by those skilled in the art, and all such modifications or changes can be seen to be included in the scope of the present invention.

Claims (8)

0.1 to 7% by weight of ceramide, 0.01 to 5% by weight of cholesterol, 0.1 to 5% by weight of fatty acids, 0.001 to 1% by weight of Acacia pollen extract and 0.1 to 10% by weight of vegetable oil non-compound But
The fatty acids, ceramides and vegetable oil non-compounds are included in a weight ratio of 1: 1-7: 1-14,
The fatty acid includes palmitic acid,
The vegetable oil non-compensation improves the skin barrier, characterized in that it comprises glycine soja oil unsaponifiables, olea europaea oil unsaponifiables, and triticum vulgare germ unsaponifiables. Cosmetic composition excellent in pilagrin active effect.
delete The method of claim 1, wherein the vegetable oil non-compensation is a skin barrier improvement and pillars, characterized in that it comprises a 1: 3 ~ 3: 0.5 ~ 2 weight ratio of soybean oil non-compensation, olive oil non-compensation and wheat germ oil non-compensation Cosmetic composition excellent in green active effect.
delete The cosmetic composition of claim 1, wherein the ceramide comprises one or more of ceramide 1, ceramide 2, ceramide 3, ceramide 4, ceramide 5, and ceramide 6.
delete The cosmetic composition of claim 1 wherein the cosmetic composition is a solution, suspension, emulsion, paste, essence, gel, cream, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation or spray A cosmetic composition having excellent skin barrier improvement and filaggrin activity, characterized in that the formulation.
The skin barrier according to claim 1, wherein the cosmetic composition is an oil-in-water (O / W), water-in-oil (W / O) or water-in-silicone (W / S) formulation. Cosmetic composition with excellent improvement and filaggrin activity.

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