KR102047073B1 - Sample preparation device and method of preparing sample using the same - Google Patents

Abstract

The sampling device includes a main body, a gene capture member, and a slide valve assembly. The main body includes a sample injection space, a sample storage tube for temporarily storing a gene sample, a storage space for receiving a residue from which the gene sample is extracted, the sample storage space, the sample storage tube, and the storage space. And a plurality of conduits connected to the. The gene capture member is disposed in the sample injection space to capture only the gene sample from the raw sample, and is separated from the gene sample when the gene sample is mixed with an extract solution. The slide valve assembly is coupled to the main body so as to be slidable, and closely adhered to the main body so that the residue, the washer solution, and the extraction solution of the raw sample disposed therein are in close contact with each other. And a plurality of connection parts formed on a surface facing the main body and controlling the connection relationship between the conduits by sliding and a sample extraction port through which the genetic sample temporarily stored in the sample storage tube is discharged.

Description

Sampling device and sampling method using same {SAMPLE PREPARATION DEVICE AND METHOD OF PREPARING SAMPLE USING THE SAME}

The present invention relates to a sampling device and a sampling method using the same, and more particularly, to a sampling device and a sampling method using the same for collecting a gene sample in the field.

Due to the development of genetic technology, various techniques for analyzing genes, which are unique markers of living organisms, have been developed. Recently, a technique for amplifying and analyzing a small amount of genes present in a sample using a polymerase chain reaction (PCR) has been widely used.

In order to test genes, it is necessary to separate and extract genes from cells, viruses, bacteria, and the like of an organism.

In order to collect the genes, only the genes must be separated after the washing of the sebaceous wall and the nuclear membrane. In the conventional method of collecting the genes, the process of centrifugation, washing, etc. takes many times, so it takes a lot of time to collect the genes themselves. do.

In addition, the process of extracting the gene or mixing the solution using a pipette, etc. in the middle of each step, a highly skilled worker is required to increase the cost.

Moreover, since centrifuges, scrubbers, reagent bottles, etc. are required, it is difficult to take a genetic sample immediately on site.

An object of the present invention is to provide a sampling device for simply collecting a genetic sample in the field.

An object of the present invention is to provide a sample collection method using the sample collection device.

Sampling device for achieving the object of the present invention as described above includes a main body, the gene capture member, and a slide valve assembly. The main body includes a sample injection space into which a raw sample is injected, a sample storage tube temporarily storing a genetic sample in the raw sample, a storage space in which a residue from which the genetic sample is extracted from the raw sample is accommodated, and It includes a sample storage space, the sample storage tube, and a plurality of conduits connected to the receiving space. The gene capture member is disposed in the sample injection space to capture only the gene sample from the raw sample, and is separated from the gene sample when the gene sample is mixed with an extract solution. The slide valve assembly is coupled to the main body so as to be slidable, and closely adhered to the main body so that the residue, the washer solution, and the extraction solution of the raw sample disposed therein are in close contact with each other. And a plurality of connection parts formed on a surface facing the main body and controlling the connection relationship between the conduits by sliding and a sample extraction port through which the genetic sample temporarily stored in the sample storage tube is discharged.

In an embodiment, the main body may further include a first accommodating space in which the extraction solution is stored, and a second accommodating space in which the washer solution is stored.

In one embodiment, it may further include an air valve coupled to the main body for applying a negative pressure or injecting air to the storage space and the sample storage tube.

In the sampling method using a sample collection device for achieving the object of the present invention as described above, the sample collection device is a sample storage space in which the raw sample is injected, a sample storage tube for temporarily storing the genetic sample in the raw sample A main body including a storage space accommodating the residue from which the genetic sample is extracted from the raw sample, and a sample storage space, the sample storage tube, and a plurality of conduits connected to the storage space; Disposed within the space to capture only the gene sample from the raw sample and when the gene sample is mixed with the extraction solution, the gene capture member is separated from the gene sample, and coupled to the main body so as to be slidable and closely adhered to the main body. Residues, wash solutions, and extraction of the raw sample disposed therein and disposed therein A sample is formed on the surface facing the main body so as not to flow out of the outside and a plurality of connection parts for controlling the connection relationship between the conduits by the sliding movement and the sample sample temporarily stored in the sample storage tube is discharged And a slide valve assembly including an outlet. In the sampling method, the raw sample is first injected into the sample injection space. Subsequently, the slide valve assembly is controlled to connect the sample injection space and the accommodation space to accommodate the residue in the accommodation space and to capture the gene sample in the gene capture member. Thereafter, a negative pressure is applied to the air valve while the washer solution is applied to the sample inlet to accommodate the rest of the residue in the storage space. Subsequently, the slide valve assembly is controlled to connect the sample injection space and the sample storage tube, and a negative pressure is applied to the air valve while the extraction solution is applied to the sample inlet to transfer the gene sample to the sample storage tube. Save it temporarily. Subsequently, the slide valve assembly is controlled to remove the negative pressure applied to the air valve. Thereafter, the slide valve assembly is controlled to connect the sample storage tube and the sample extraction port to discharge the gene sample.

In one embodiment, the step of removing the negative pressure may introduce external air through the sample extraction port.

In one embodiment, the step of temporarily storing the gene sample in the sample storage tube, the step of applying the sound pressure from the top of the sample injection space; Manually introducing the extract solution from the upper portion of the sample injection space by using the negative pressure; And using the extraction solution may comprise the step of wetting the gene capture member disposed on the lower portion of the sample injection space from the top surface.

According to the embodiment of the present invention as described above, it is possible to simply capture the gene using the gene capture member, there is no need for a centrifuge. In addition, the process of gene capture, washing, etc. can be performed in an integrated sample collection device, and a separate reagent container is not necessary, so that a genetic sample can be immediately taken in a harsh environment such as a site.

In addition, since the extraction solution and the washing solution are automatically flowed out by the slide valve assembly and air pressure (or negative pressure), the arrangement of the first storage space and the second storage space is not limited. Therefore, contamination of the extraction solution and the washer solution is prevented, and the design of the sample extraction device is free.

In addition, the time taken to collect the genetic sample is reduced, and even without special technology, anyone can easily collect the genetic sample and the cost is reduced.

Figure 1 is a perspective image of the ready state of the sample collection device according to an embodiment of the present invention looking from the front right direction.
FIG. 2 is a perspective image of a sampling state of the sample collection device illustrated in FIG. 1 as viewed from the front right direction.
3 is a perspective view of the main body of the sampling device shown in FIG. 1 from above.
Figure 4 is a perspective view of the slide valve assembly of the sampling device shown in Figure 1 from the top.
5 is a flow chart showing a sample collection method using a sample collection device according to an embodiment of the present invention.
6, 8, 10, 12, 14, and 16 is a perspective view of the sample collection device used in the sample collection method shown in FIG.
7, 9, 11, 13, 15, and 17 are cross-sectional views showing a sampling device used in the sampling method shown in FIG.
18 is a perspective view of the main body of the sampling device according to another embodiment of the present invention viewed from above.
19 is a perspective view from above of a slide valve assembly coupled to the main body shown in FIG. 18.
FIG. 20 is a perspective view from above of a step of removing sound pressure in a sampling method using the sampling device shown in FIGS. 18 and 19.

With respect to the embodiments of the present invention disclosed in the text, specific structural to functional descriptions are merely illustrated for the purpose of describing embodiments of the present invention, embodiments of the present invention may be implemented in various forms and It should not be construed as limited to the embodiments described in.

As the present invention allows for various changes and numerous embodiments, particular embodiments will be illustrated in the drawings and described in the text. However, this is not intended to limit the present invention to a specific disclosed form, it should be understood to include all modifications, equivalents, and substitutes included in the spirit and scope of the present invention. In describing the drawings, similar reference numerals are used for the components.

Terms such as first and second may be used to describe various components, but the components should not be limited by the terms. The terms are used only for the purpose of distinguishing one component from another. For example, without departing from the scope of the present invention, the first component may be referred to as the second component, and similarly, the second component may also be referred to as the first component.

When a component is referred to as being "connected" or "connected" to another component, it may be directly connected to or connected to that other component, but it may be understood that other components may be present in between. Should be. On the other hand, when a component is said to be "directly connected" or "directly connected" to another component, it should be understood that there is no other component in between. Other expressions describing the relationship between components, such as "between" and "immediately between," or "neighboring to," and "directly neighboring to" should be interpreted as well.

The terminology used herein is for the purpose of describing particular example embodiments only and is not intended to be limiting of the present invention. Singular expressions include plural expressions unless the context clearly indicates otherwise. In this application, the terms "comprise" or "have" are intended to indicate that there is a feature, number, step, operation, component, part, or combination thereof that has been described, and that one or more of them is present. It is to be understood that it does not exclude in advance the possibility of the presence or addition of other features or numbers, steps, actions, components, parts or combinations thereof.

Unless defined otherwise, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art. Terms such as those defined in the commonly used dictionaries should be construed as having meanings consistent with the meanings in the context of the related art and shall not be construed in ideal or excessively formal meanings unless expressly defined in this application. Do not.

Hereinafter, with reference to the accompanying drawings, it will be described in detail a preferred embodiment of the present invention. The same reference numerals are used for the same elements in the drawings, and duplicate descriptions of the same elements are omitted.

1 is a perspective image of the sample collection device according to an embodiment of the present invention looking in the front right direction, and FIG. 2 is a perspective view of the sample collection device shown in FIG. 1 in a front right direction. 3 is a perspective view of the main body of the sampling device shown in FIG. 1 from above, and FIG. 4 is a perspective view of the slide valve assembly of the sampling device shown in FIG. 1 from above. . For convenience of description, the components shown in FIGS. 1 to 4 are both exposed to the outside of the slide valve assembly and disposed inside the slide valve assembly and the main body. In the following detailed description, the exposure to the outside of the slide valve assembly and the components disposed inside the slide valve assembly and the main body are distinguished and described. Thus, those skilled in the art will appreciate the following detailed description. Through the present invention will be able to grasp in detail.

1 to 4, the sampling device includes a main body 100, a gene trap 130, and a slide valve assembly 200.

The main body 100 has a rectangular parallelepiped shape, and the slide valve assembly 200 is slidably coupled to the upper portion of the main body 100 to slide in a long axis direction according to a sampling step. For example, the main body 100 may include chemically stable and rigid synthetic resins, metals, metal oxides, ceramics, PDMS, and the like so that genetic samples are not modified. In one embodiment, the main body 100 may include a transparent or translucent material to observe the movement of the raw sample, the genetic sample, the washing solution, the buffer solution, and the like from the outside.

In the sample collection device according to the present embodiment, the genetic sample is extracted by injecting a raw material sample (Lysis) into the liquid wall and nuclear membrane in liquid form.

The main body 100 includes a first storage space 112, a second storage space 114, a third storage space 116, a sample injection space 120, a first conduit 152, and a second conduit 154. And a third conduit 156, a sample storage tube 158, an air valve 165, a guide bar 50, and a guide rail 60.

The first accommodating space 112 is disposed inside the main body 100 and stores an extraction buffer used to extract genes. The extraction solution (elution buffer) includes a material that breaks the binding of the gene capture unit 130 and the genetic sample, such as distilled water, EDTA, and the like.

The first accommodating space 112 is connected to the first air inlet 112a and the extraction solution outlet 112b which are opened upward. In the present embodiment, the air is introduced into the first air inlet 112a and not a method of applying air pressure into the first accommodating space 112. A method of passively introducing air through the air inlet 112a is used. In another embodiment, a method of actively introducing air into the first air inlet 112a and applying air pressure into the first accommodating space 112 may be used. When the sound pressure is transferred into the first storage space 112, the extraction solution in the first storage space 112 is discharged toward the extraction solution outlet 112b.

In this embodiment, the air is injected into the first air inlet 112a, and the extraction solution is not discharged to the extraction solution outlet 112b, but is applied to the air valve 165 to be delivered toward the sample injection space 120. A negative pressure is applied to the first storage space 112 through the extraction solution outlet 112b so that the extraction solution flows out. When the extraction solution flows out of the first storage space 112 through the extraction solution outlet 112b by the negative pressure, external air is passively introduced into the first air inlet 112a. If there is no first air inlet 112a, the sound pressure applied to the first accommodating space 112 may not be released and the extraction solution may not be smoothly discharged.

The second accommodating space 114 is disposed inside the main body 100 to store a washing buffer used to remove the residual material of the raw sample. Washing buffer (washing buffer) is a genetic sample, such as ethanol, while maintaining the state attached to the gene capture unit 130 includes a substance that is removed from the sample.

The second accommodating space 114 is connected to the second air inlet 114a and the washer solution outlet 114b which are opened upward. In this embodiment, the air is not actively applied to the second air inlet 114a to apply air pressure into the second accommodating space 114, but a negative pressure is applied to the air valve 165. A method of passively introducing air through the second air inlet 114a is used. In another embodiment, a method of injecting air into the second air inlet 114a and applying air pressure into the second accommodating space 114 may be used.

In this embodiment, the air is injected into the second air inlet 114a, and the washing solution is not discharged to the washing solution outlet 114b, but is applied to the air valve 165 to be delivered toward the sample injection space 120. The negative pressure is applied to the second storage space 114 through the washer solution outlet 114b so that the washer solution flows out. When the washer solution flows out of the second accommodating space 114 through the washer solution outlet 114b by the negative pressure, external air is passively introduced into the second air inlet 114a. If there is no second air inlet 114a, the sound pressure applied to the second accommodating space 114 may not be released and the washer solution may not be smoothly discharged.

When a negative pressure is applied to the second accommodating space 114, the washer solution in the second accommodating space 114 flows out to the washer solution outlet 114b and is discharged.

The third accommodating space 116 is disposed inside the main body 100 and stores the washing solution and the buffer solution that have passed through the gene trap 130. The third receiving space 116 is connected to the other end of the second conduit 154 and the air valve 165.

In the present embodiment, the third storage space 116 is formed inside the main body 100 so as not to be exposed to the outside, and the used washing solution and buffer solution are stored, and then discarded after the extraction of the genetic sample is completed.

The sample injection space 120 is opened toward the upper surface of the main body 100, and the raw sample from which the thin wall and the nuclear membrane are destroyed (Lysis), the washer solution stored in the second storage space 114, and the first storage space. The extraction solution stored at 112 is injected. In this embodiment, the raw sample is first injected into the sample injection space 120, and then the washer solution and the extraction solution are sequentially injected. The injection of the raw sample, the washing solution, and the extraction solution may be automatically flowed out by the air valve (or negative pressure) applied from the slide valve assembly 200 and the outside, without a separate pipette (not shown).

In this embodiment, the extraction solution and the washer solution is automatically flowed out and injected by the slide valve assembly 200 and the air pressure (or negative pressure), so that the arrangement of the first storage space 112 and the second storage space 114 is It is not limited. Therefore, contamination of the extraction solution and the washer solution is prevented, and the design of the sample extraction device is free.

Gene capture member 130 is disposed in the lower portion of the sample injection space 120 to selectively capture only the gene sample from the injected raw sample. The gene capture member 130 includes a material that captures a gene, such as magnetic material, glass, silicon, synthetic resin, and the like. In the present embodiment, the gene capture member 130 is processed in the form of a porous medium so that the gene sample is captured by the gene capture member 130 while the raw sample, the washing solution, etc. pass downward. For example, the gene trap member 130 may form a porous medium by filling or binding bead-shaped particles.

The first conduit 152 is disposed inside the main body 100, one end of which is connected to the lower portion of the sample injection space 120, and the first terminal 152a disposed at the other end is opened upward. In this embodiment, one end of the first conduit 152 is disposed under the gene capture member 130, the residual material of the raw sample, the washer solution, and the extraction solution passed through the gene capture member 130 1 is delivered to the slide valve assembly 200 through the terminal (152a).

In this embodiment, one first terminal 152a is formed at the other end of the first conduit 152. The first terminal 152a of the first conduit 152 is connected to the first sample injection connection 225 as the slide valve assembly 200 moves. The distance that the first terminal 152a of the first conduit 152 is spaced apart from the bottom surface of the main body 100 is such that one end of the first sample injection connection 225 is spaced apart from the bottom surface of the slide valve assembly 200. As the distance, the first terminal 152a of the first conduit 152 may be connected to the first sample injection connection 225 of the slide valve assembly 200.

The second conduit 154 is disposed inside the main body 100 and has a second terminal 154a formed at one end thereof. The second terminal 154a of the second conduit 154 is connected to the first sample of the first conduit 152 through the first sample injection connection 225 of the slide valve assembly 200 as the slide valve assembly 200 moves. It is connected to the terminal 152a. When the first terminal 152a of the first conduit 152 is connected to one end of the first sample injection connection 225, the second terminal 154a of the second conduit 154 is connected to the first sample injection connection ( It is connected to the other end of the 225, the first conduit 152 and the second conduit 154 is connected to each other through the first sample injection connection 225. The distance that the second terminal 154a of the second conduit 154 is spaced apart from the bottom surface of the main body 100 is such that the other end of the first sample injection connection 225 is spaced apart from the bottom surface of the slide valve assembly 200. As the distance, the second terminal 154a of the second conduit 154 may be connected to the other end of the first sample injection connection 225.

The other end of the second conduit 154 is connected to the third receiving space 116. When the first sample injection connection 225 of the slide valve assembly 200 connects the first conduit 152 and the second conduit 154, the first sample injection connection 225 passes through the gene capture member 130 of the sample injection space 120. The residual material and the washer solution of the raw sample are injected into the third storage space 116 after sequentially passing through the first conduit 152, the first sample injection connection 225, and the second conduit 154.

The third conduit 156 is disposed inside the main body 100, one end of which is connected to the sample storage tube 158, and the other end of which is connected to the air valve 165. The third conduit 156 is used as a passage through which external air flows.

The sample storage tube 158 is disposed inside the main body 100 to temporarily store the genetic sample. One end of the sample storage tube 158 is connected to the third conduit 156 and the other end of the sample storage tube terminal 158a is formed. The sample storage tube terminal 158a of the sample storage tube 158 is connected to the second extraction connection portion 285 or the sample extraction connection conduit 272 of the slide valve assembly 200 according to the movement of the slide valve assembly 200. .

The gene sample is introduced into the sample storage tube 158 through the first conduit 152 of the main body 100, the second extraction connection portion 285 of the slide valve assembly 200, and the sample storage tube terminal 158a. After being temporarily stored, the sample is discharged to the sample extraction port 270 through the sample storage tube terminal 158a and the sample extraction connection conduit 272.

The distance between the sample storage tube terminal 158a of the sample storage tube 158 spaced apart from the bottom surface of the main body 100 is that the other end of the second extraction connection portion 285 is spaced apart from the bottom surface of the slide valve assembly 200. Distance and one end of the sample extraction connection conduit 272 is equal to the distance spaced from the bottom surface of the slide valve assembly 200, so that the sample storage tube terminal 158a of the sample storage tube 158 has a second extraction connection portion. It may be connected to the other end of 285 or one end of the sampling connection conduit 272.

The guide bar 50 is disposed above the main body 100 to restrain the slide valve assembly 200 from moving vertically. In this embodiment, the guide bar 50 extends in a direction perpendicular to the direction in which the slide valve assembly 200 slides and covers a portion of the upper surface of the slide valve assembly 200.

The guide rail 60 is disposed above the main body 100 to guide the slide valve assembly 200 to slide. In the present embodiment, the guide rail 60 extends along the sliding direction of the slide valve assembly 200 and covers both sides of the slide valve assembly 200.

The slide valve assembly 200 has a flat plate shape and slides on an upper portion of the main body 100 and covers an upper surface of the main body 100. For example, the slide valve assembly 200 may include materials such as synthetic resin, metal, metal oxide, ceramic, PDMS, and the like that are chemically stable and rigid so that genetic samples are not modified. In one embodiment, the slide valve assembly 200 may include a transparent or translucent material to observe the movement of the raw sample, the genetic sample, the washing solution, the buffer solution, and the like from the outside.

For example, between the upper surface of the main body 100 and the slide valve assembly 200, the lubricating oil 201, silicon, etc. are disposed to prevent the inflow of external air or the outflow of genetic samples, wash solution, extraction solution, etc. It may be.

In the present exemplary embodiment, the slide valve assembly 200 includes a sample inlet 220, a first sample inlet connection 225, a first air inlet 241, a first washer solution connection 243, and a second washer solution connection ( 245, the second air inlet 261, the first extraction solution connection 263, the second extraction solution connection 265, the sample extraction port 270, the sample extraction connection conduit 272, the third air inlet 281 ), A first extraction connector 283, and a second extraction connector 285.

The sample inlet 220 is formed by penetrating the slide valve assembly 200 in the vertical direction, and becomes a passage through which a raw sample (not shown) is injected into the sample injection space 120. The distance from which the sample inlet 220 is spaced apart from the bottom surface of the slide valve assembly 200 is the same as the distance from which the sample injection space 120 is spaced apart from the bottom surface of the main body 100, thereby positioning the slide valve assembly 200. By adjusting the sample inlet 220 may be disposed on the sample injection space (120).

The first sample injection connection 225 has a recess shape that is exposed on the rear surface of the slide valve assembly 200 and extends in an oblique direction. The first sample injection connector 225 connects the first conduit 152 and the second conduit 154 to be injected through the sample inlet 220 and the residual material of the raw sample passed through the gene capture member 130 is formed. The first conduit 152, the first sample injection connection 225, and the second conduit 154 may be moved to the third storage space 116.

In this embodiment, since the step of injecting the raw sample is performed first, the sample inlet 220 and the first sample injection connection 225 is disposed on the rightmost side in the slide valve assembly 200. In another embodiment, the sample inlet 220 and the first sample inlet connection 225 may be disposed on the leftmost side of the slide valve assembly 200.

The first air inlet 241 is formed by penetrating the slide valve assembly 200 in the vertical direction and becomes a passage through which external air flows into the second receiving space 114. The distance that the first air inlet 241 is spaced apart from the bottom surface of the slide valve assembly 200 is the same as the distance from which the second air inlet 114a is spaced apart from the bottom surface of the main body 100. The first air inlet 241 may be disposed on the second air inlet 114a by adjusting the position of 200.

The first washer solution connection part 243 has a recess shape exposed on the rear surface of the slide valve assembly 200 and extending in an inclined direction. The first washer solution connection part 243 connects the second storage space 114 and the sample injection space 120 to the washer solution 44a stored in the second storage space 114 to the sample injection space 120. I can move it. Specifically, one end of the first washer solution connection part 243 is connected to the washer solution outlet 114b of the second receiving space 114 and the other end of the first washer solution connection part 243 is the sample injection space 120 Is connected to.

The distance at which one end of the first washer solution connection part 243 is spaced apart from the bottom of the slide valve assembly 200 is equal to the distance that the washer fluid outlet 114b is spaced apart from the bottom of the main body 100 and the first washer The distance that the other end of the solution connecting portion 243 is spaced apart from the bottom surface of the slide valve assembly 200 is equal to the distance that the upper portion of the sample injection space 120 is spaced from the bottom surface of the main body 100, The first washing solution connecting portion 243 may be disposed between the washing solution outlet 114b and the sample injection space 120 by adjusting the position of the 200.

The second washer solution connection part 245 has a recess shape that is exposed on the rear surface of the slide valve assembly 200 and extends in an oblique direction. The second washer solution connection part 245 is connected to the first conduit 152 and the second conduit 154 and included in the washer solution and the washer solution injected through the sample inlet 220 and passed through the gene capture member 130. The residual material may move to the third storage space 116 through the first conduit 152, the first sample injection connection 225, and the second conduit 154.

In the present embodiment, the step of injecting the washer solution is performed after the step of injecting the raw sample, so that the first air inlet 241, the first washer solution connection part 243, and the second washer solution connection part 245 are sampled. The injection hole 220 and the first sample injection connection 225 is disposed immediately next to.

The second air inlet 261 is formed by penetrating the slide valve assembly 200 in the vertical direction and becomes a passage through which external air flows into the first receiving space 112. The distance from which the second air inlet 261 is spaced apart from the bottom of the slide valve assembly 200 is equal to the distance from which the first air inlet 112a is spaced apart from the bottom of the main body 100. The second air inlet 261 may be disposed on the first air inlet 112a by adjusting the position of 200.

The first extraction solution connecting portion 263 has a recess shape exposed on the rear surface of the slide valve assembly 200 and extending in an inclined direction. The first extraction solution connecting portion 263 connects the first storage space 112 and the sample injection space 120 to extract the extraction solution 46a stored in the first storage space 112 to the sample injection space 120. I can move it. Specifically, one end of the first extraction solution connecting portion 263 is connected to the extraction solution outlet 112b of the first receiving space 112 and the other end of the first extraction solution connecting portion 263 is the sample injection space 120 Is connected to.

The distance that one end of the first extraction solution connecting portion 263 is spaced apart from the bottom surface of the slide valve assembly 200 is equal to the distance that the extraction solution outlet 112b is spaced apart from the bottom surface of the main body 100 and the first extraction is performed. The distance that the other end of the solution connecting portion 263 is spaced apart from the bottom of the slide valve assembly 200 is equal to the distance that the top of the sample injection space 120 is spaced from the bottom of the main body 100, the slide valve assembly The first extraction solution connecting portion 263 may be disposed between the extraction solution outlet 112b and the sample injection space 120 by adjusting the position of the 200.

The second extraction solution connecting portion 265 has a recess shape which is exposed on the rear surface of the slide valve assembly 200 and extends in an oblique direction. The second extraction solution connecting portion 265 connects the upper portion of the sample injection space 120 and the sample storage tube 158. In another embodiment, when air is introduced through the second air inlet 261, the extraction solution 46a in the first accommodating space 112 flows into the sample injecting space 120 to be in the sample injecting space 120. Pressure may also be applied to existing air. The second extraction solution connection part 265 solves the negative pressure generated while the extraction solution 46a flows into the sample injection space 120 and is injected, so that the extraction solution 46a is smoothly injected into the sample injection space 120. do. In this embodiment, the negative pressure is first applied to the air valve 165, the negative pressure is transmitted through the connecting portion 265, the negative pressure is also applied to the sample injection space 120. A negative pressure applied to the sample injection space 120 sucks the extraction solution 46a in the first storage space 112, and the extraction solution 46a passes through the sample extraction space 120 through the first extraction solution connection part 263. Come into the soaking gene capture member 130. In order to fill the space in which the extraction solution 46a has exited, the external air is manually introduced into the first storage space 112 through the second air inlet 261.

In this embodiment, since one end of the second extraction solution connecting portion 265 is disposed above the sample injection space 120, the extraction solution 46a or the gene sample (46 of FIG. 12) is not introduced, and the sample injection is performed. Only the excess air present in the space 120 is moved.

The extraction solution introduced into the sample injection space 120 through the first extraction solution connection part 263 penetrates into the void of the gene capture member 130 and the gene sample is separated from the gene capture member 130. For example, the extraction solution is dripping from the upper portion of the sample injection space 120 in the form of droplets to wet the gene capture member 130, and the gene capture member 130 wetted by the extraction solution at room temperature for about one minute. By maintaining it, the target gene can be separated from the gene capture member 130. In another embodiment, in order to increase the separation efficiency of the target gene, the gene capture member 130 moistened by the extraction solution is heated to a predetermined temperature (for example, about 56 ° C.) and maintained for about 1 minute. It may be separated from the gene trap member 130.

In the present embodiment, the step of injecting the extraction solution is performed after the step of injecting the washer solution, so that the second air inlet 261, the first extraction solution connection part 263, and the second extraction solution connection part 265 are made of the first solution. It is disposed next to the first air inlet 241, the first washer solution connection part 243, and the second washer solution connection part 245.

The third air inlet 281 is formed to penetrate the slide valve assembly 200 in the vertical direction and is connected to one end of the first extraction connection portion 283 to be a passage through which external air flows into the sample injection space 120. .

The first extraction connector 283 is exposed on the rear surface of the slide valve assembly 200 and has a recess shape connecting the third air inlet 281 and the sample injection space 120. The first extraction connection portion 283 is a passage through which external air flows into the sample injection space 120 to fill the space generated by the genetic sample 46 is discharged from the sample injection space 120.

The second extraction connector 285 connects the first conduit 152 and the sample storage tube 158. Specifically, one end of the second extraction connector 285 is connected to the first terminal 152a of the first conduit 152, and the other end is connected to the sample storage tube terminal 158a of the sample storage tube 158. . Since one end of the first conduit 152 is connected to the lower portion of the sample injection space 120, the material passing through the first conduit 152 includes the gene sample 46 extracted from the gene trapping member 130. do.

When a negative pressure is applied to the air valve 165, the negative pressure is captured through the third conduit 156, the sample storage tube 158, the second extraction connection 285, and the first conduit 152. It is applied to the bottom of the member 130. When a negative pressure is applied to the lower portion of the gene capture member 130, the gene sample 46 separated from the gene capture member 130 by the extraction solution 46a is the first conduit 152 and the second extraction connector 285. It moves to the sample storage pipe 158 through.

Since the gene capture member 130 includes a porous medium, a very high level of negative pressure is applied to the air valve 165 to extract the gene sample 46. When excessive negative pressure is applied to the air valve 165, the gene sample 46 passes through the sample storage tube 158 to the third conduit 156.

In the present embodiment, the step of extracting the gene sample is performed after the step of injecting the extraction solution, so that the third air inlet 281, the first extraction connector 283, and the second extraction connector 285 are the second air inlet. 261, the first extraction solution connecting portion 263, and the second extraction solution connecting portion 265.

The sample extraction port 270 is formed by penetrating the slide valve assembly 200 in the vertical direction and is connected to one end of the sample connection connection conduit 272 so that external air flows into the second conduit 154 or the gene sample 46 ) Is a passage through which it is discharged to the outside.

The sample connection connection conduit 272 is exposed on the rear surface of the slide valve assembly 200 and the sample storage port 270 and the second terminal 154a or the sample extraction port 270 of the second conduit 154 and the sample storage. It has a recess shape for connecting the sample storage tube terminal 158a of the tube 158. The sample extraction connection conduit 272 serves as a passage through which external air flows into the second storage space 114 connected to the second conduit 154 to remove the negative pressure applied to the air valve 165, or the sample storage pipe ( Genetic sample 46 stored in 158 is discharged.

In the present embodiment, the step of removing the negative pressure of the air valve 165 and the step of discharging the gene sample is performed after the step of extracting the gene sample, so that the sample extraction port 270 and the sample extraction connection conduit 272 are removed. It is disposed next to the third air inlet 281, the first extraction connector 283, and the second extraction connector 285.

5 is a flow chart showing a sampling method using a sampling device according to an embodiment of the present invention, Figure 6, Figure 8, Figure 10, Figure 12, Figure 14, and Figure 16 is a sample collection shown in FIG. 7 is a perspective view of a sampling device used in the method from above, and FIGS. 7, 9, 11, 13, 15, and 17 illustrate a sampling device used in the sampling method shown in FIG. It is a cross section. For convenience of description, the components shown in FIGS. 6, 8, 10, 12, 14, and 16 are exposed to the outside of the main body and the slide valve assembly and inside the main body and the slide valve assembly. All arrangements are shown. In the following detailed description, since the main body and the slide valve assembly are exposed to the outside and distinguished from the components disposed therein, those skilled in the art will recognize the present invention through the following detailed description. You will be able to identify this in detail.

FIG. 6 is a perspective view of a sample collection device viewed from above in a step of injecting a raw sample and capturing a gene, and FIG. 7 is a cross-sectional view of the sample collection device illustrated in FIG. 6.

1 to 7, first, a raw sample is prepared by destroying a cell wall and a nuclear membrane of a biological sample including tissues, blood, and microorganisms of an organism. For example, it is possible to destroy the cell wall and the nuclear membrane of a biological sample using ultrasonic waves, surfactants and the like.

By adjusting the slide valve assembly 200, the sample inlet 220 is disposed on the sample injection space 120, the first sample injection connection 225 is connected to the first conduit 152 and the second conduit 154 Connect it. For example, when the sampling device is released for the first time, the sample inlet 220 is disposed on the sample injection space 120, and one end of the first sample injection connection 225 is connected to the first conduit 152. The other end corresponds to the terminal 152a and the other end corresponds to the second terminal 154a of the second conduit 154, and the remaining first air inlet 112a, the extraction solution outlet 112b, and the second air inlet ( 114a), the washer solution outlet 114b, and the sample storage tube terminal 158a are blocked by the flat rear surface of the slide valve assembly 200.

Then, the prepared raw sample is injected into the sample injection space 120 (step S110). In this embodiment, the raw sample may be injected into the sample injection space 120 using a pipette or the like. In another embodiment, the raw sample may be generated by injecting a biological sample and a surfactant into the sample injection space 120.

After that, only the gene sample is captured from the raw sample using the gene capture member 130 (step S120).

Specifically, when the negative pressure 52 is applied through the air valve 165 while the first conduit 152 and the second conduit 154 are connected, the third receiving space 116 and the second conduit 154 are provided. The negative pressure 52 is transmitted to the lower portion of the sample injection space 120 through the first sample injection connection 225 and the first conduit 152.

When the negative pressure 52 is applied to the lower portion of the sample injection space 120, only the gene sample from the raw sample is attached to the gene capture member 130, and the residual material 42 is the first conduit 152, the first sample injection connection portion. 225 and sequentially pass through the second conduit 154 is received in the third receiving space (116).

FIG. 8 is a perspective view of a sampling device viewed from above in a step of removing residual material, and FIG. 9 is a cross-sectional view of the sampling device shown in FIG. 8.

1 to 6, 8, and 9, the residual material 42 in the sample injection space 120 is subsequently removed (step S130).

Specifically, the slide valve assembly 200 is pushed to the right to move the first air inlet 241 onto the second air inlet 114a of the second accommodating space 114, and the first washer solution connection part ( 243 is used to connect the washer solution outlet 114b of the second receiving space 114 and the sample injection space 120, and the first conduit 152 of the first conduit 152 using the second washer solution connection part 245. Terminal 152a is coupled to second terminal 154a of second conduit 154.

When the negative pressure is applied to the air valve 165, the negative pressure is applied to the third storage space 116, the second conduit 154, the second washer solution connection 245, the first conduit 152, the sample injection space 120, And the washer solution 44a in the second storage space 114 through the first washer solution connection part 243. When the washing solution 44a moves under negative pressure, air flows in through the first air inlet 241.

Residual material 44 other than the gene sample captured by the washer solution 44a introduced into the sample injection space 120 passes through the first conduit 152 and the second washer solution connection part 245 to receive the third material. Move to space 116. The air 54, which previously filled the third storage space 116, is discharged to the outside through the air valve 165.

Injecting the washing solution 44a may be a method of applying a negative pressure to the air valve 165 or a method of injecting air into the first air inlet 241.

FIG. 10 is a perspective view of a sample collection device viewed from above in a step of extracting a gene sample, and FIG. 11 is a cross-sectional view of the sample collection device shown in FIG. 10.

1 to 6, 10, and 11, the extraction solution 46a in the first accommodating space 112 is continuously injected into the sample injection space 120 to extract the gene sample (46 in Fig. 12). (Step S140).

Specifically, the slide valve assembly 200 is pushed in the right direction to move the second air inlet 261 onto the first air inlet 112a of the first accommodating space 112, and the first extraction solution connecting part ( 263 is used to connect the extraction solution outlet 112b of the first storage space 112 and the sample injection space 120, and the sample injection space 120 using the second extraction solution connection part 265. The first terminal 152a of 152 is connected to the second terminal 154a of the second conduit 154.

When the negative pressure is applied to the air valve 165, the negative pressure is the third conduit 156, the sample storage tube 158, the second extraction solution connection portion 265, the upper portion of the sample injection space 120, and the first extraction solution. The extraction solution 46a is applied to the first storage space 112 through the connection part 263. When the extraction solution 46a moves under negative pressure, air is introduced through the second air inlet 261.

When air is introduced through the second air inlet 261, the extraction solution 46a disposed in the second accommodation space 114 is injected into the sample injection space 120. The gene sample attached to the gene capture member 130 is separated by the extraction solution 46a injected into the sample injection space 120 to float in the extraction solution 46a.

In this embodiment, both the second extraction solution connecting portion 265 for applying a negative pressure to the sample injection space 120 and the first extraction solution connecting portion 263 for supplying the extraction solution 46a to the sample injection space 120 are all present. Since it is located above the sample injection space 120, the extraction solution 46a does not flow into the sample storage tube 158, but the upper surface of the gene capture member 130 located at the bottom of the sample injection space 120 Start to soak.

Injecting the extraction solution 46a may be a method of applying a negative pressure to the air valve 165 or a method of injecting air into the second air inlet 261.

12 is a perspective view of a sample collection device viewed from above in a step of extracting a gene sample and storing the extracted gene sample in a sample storage tube, and FIG. 13 is a cross-sectional view of the sample collection device shown in FIG. 12.

Referring to FIGS. 1 to 6, 12, and 13, the genetic sample 46 separated and extracted from the gene trap member 130 by the extraction solution 46a is temporarily stored in the sample storage tube 158. (Step S150).

Specifically, the slide valve assembly 200 is pushed in the right direction to move the third air inlet 281 onto the first air inlet 112a of the first accommodating space 112, and the second extraction connector 283. And the other end of the first to the sample injection space 120, and the ends of the second extraction connecting portion 285 to the first terminal 152a of the first conduit 152 and the sample storage tube of the sample storage tube 158 Move to terminal 158a.

When a negative pressure is applied to the air valve 165, the negative pressure is collected through the third conduit 156, the sample storage tube 158, the second extraction connector 285, and the lower portion of the sample injection space 120. And applied to the genetic sample 46 isolated from 130).

Gene sample 46 is moved into the sample reservoir 158 by the negative pressure.

In this embodiment, the movement of the genetic sample 46 was used to apply a negative pressure to the air valve 165. In another embodiment, a method of injecting air into the first air inlet 281 may be used.

FIG. 14 is a perspective view of a sample collection device viewed from above in a step of removing a negative pressure applied to a gene sample in a sample storage tube, and FIG. 15 is a cross-sectional view of the sample collection device illustrated in FIG. 14.

1 to 6, 14, and 15, the negative pressure applied to the gene sample 46 is subsequently removed (step S160).

Since the gene capture member 130 includes a porous medium, a very high level of negative pressure is applied to the air valve 165 to move the gene sample 46 to the sample storage tube 158. When excessive negative pressure is applied to the air valve 165, it becomes difficult to discharge the genetic sample 46 in a later step, so that the external air 59 flows in the opposite direction to the direction in which the genetic sample 46 has moved, thereby reducing the negative pressure. Remove That is, when the sample storage tube terminal 158a is connected to the outside in the state in which the negative pressure is present in the third conduit 156 in the state in which the gene sample 46 fills the sample storage tube 158, the external air due to the negative pressure Is rapidly introduced into the sample storage pipe 158 through the sample storage pipe terminal 158a. When the external air is rapidly introduced into the sample storage tube 158, the genetic sample 46 exits toward the air valve 165 along the third conduit 156. In this embodiment, the negative pressure in the sample storage tube 158 is removed to prevent the genetic sample 46 from being lost by escaping toward the air valve 165.

Specifically, the slide valve assembly 200 is pushed in the right direction to connect one end of the sampling connection conduit 272 to the second terminal 154a of the second conduit 154.

Since the sample extraction connection conduit 272 is connected to the outside through the sample extraction port 270, the external air 57 is introduced through the sample extraction connection conduit 272. The introduced external air 57 flows into the third conduit 156 through the second conduit 154, the third accommodation space 116, and the air valve 165. The negative pressure applied to the genetic sample 46 in the previous step is removed by the external air 57 introduced into the third conduit 156.

FIG. 16 is a perspective view of a sample collection device viewed from above in a step of discharging a gene sample in a sample storage tube, and FIG. 17 is a cross-sectional view of the sample collection device illustrated in FIG. 16.

1 to 6, 16, and 17, finally, the gene sample 46 temporarily stored in the sample storage tube 158 is discharged (step S170).

Specifically, the slide valve assembly 200 is pushed in the right direction to connect one end of the sample extraction connection conduit 272 to the sample storage tube terminal 158a.

When the air 59 is injected through the air valve 165, the pressure is applied to the genetic sample 46 that is temporarily stored in the sample storage tube 158, and is discharged to the outside through the sample extraction port 270.

18 is a perspective view of the main body of the sample collection device according to another embodiment of the present invention from above, and FIG. 19 is a perspective view of the slide valve assembly coupled to the main body of FIG. 18, viewed from above. In the present embodiment, the remaining components except for the negative pressure removing conduit and the negative pressure removing hole are the same as those shown in FIGS. 1 to 17, and thus redundant descriptions of the same components will be omitted.

18 and 19, the sampling device includes a main body 300, a gene trap 130, and a slide valve assembly 400.

The main body 300 has a rectangular parallelepiped shape, the slide valve assembly 400 is slidingly coupled to the upper portion of the main body 300 and slides in the long axis direction according to the sampling step.

The main body 300 includes a first storage space 112, a second storage space 114, a third storage space 116, a sample injection space 120, a first conduit 152, and a second conduit 154. And a third conduit 156, a sample storage tube 158, a negative pressure removing conduit 159, an air valve 165, a guide bar 50, and a guide rail 60.

The negative pressure removing conduit 159 is connected to the air valve 165 through the third conduit 156. In this embodiment, the negative pressure removing conduit 159 is formed inside the main body 300, and includes a negative pressure removing tube terminal 159a at the top. The negative pressure removing tube terminal 159a is opened toward the upper surface of the main body 300 and connected to the negative pressure removing hole 267 of the slide valve assembly 400.

The negative pressure removing conduit 159 allows external air to flow into the third conduit 156 to remove the negative pressure applied to the sample storage tube 158.

The slide valve assembly 400 has a flat plate shape and slides on an upper portion of the main body 300 and covers an upper surface of the main body 300.

The slide valve assembly 400 includes a sample inlet 220, a first sample inlet connection 225, a first air inlet 241, a first washer solution connection 243, a second washer solution connection 245, and a second. Air inlet 261, first extraction solution connection 263, second extraction solution connection 265, negative pressure removal hole 267, sample extraction port 270, sample extraction connection conduit 272, third air inlet 281, a first extraction connector 283, and a second extraction connector 285.

The distance from which the negative pressure removing hole 267 is spaced apart from the bottom of the slide valve assembly 400 is equal to the distance from which the negative pressure removing tube terminal 159a is spaced apart from the bottom of the main body 300. When the negative pressure removing hole 267 moves above the negative pressure removing tube terminal 159a, external air flows into the negative pressure removing conduit 159 to remove the negative pressure applied to the sample storage tube 158.

In this embodiment, the negative pressure removing conduit 159 is disposed on the opposite side of the third receiving space 116 with respect to the air valve 165. When the negative pressure removing conduit 159 is disposed on the opposite side of the third storage space 116 based on the air valve 165, the residue disposed in the third storage space 116 in the process of removing the negative pressure stores the sample. Movement toward the tube 158 is contaminated to contaminate the genetic sample 46.

FIG. 20 is a perspective view from above of a step of removing sound pressure in a sampling method using the sampling device shown in FIGS. 18 and 19.

5 to 7, 18, and 19, first, a raw sample is prepared by destroying a cell wall and a nuclear membrane of a biological sample including tissues, blood, and microorganisms of an organism.

By adjusting the slide valve assembly 200, the sample inlet 220 is disposed on the sample injection space 120, the first sample injection connection 225 is connected to the first conduit 152 and the second conduit 154 Connect it.

Then, the prepared raw sample is injected into the sample injection space 120 (step S110).

After that, only the gene sample is captured from the raw sample using the gene capture member 130 (step S120).

5, 6, 8, 9, 18, and 19, the residual material 42 in the sample injection space 120 is subsequently removed (step S130).

Referring to FIGS. 5, 6, 10, 11, 18, and 19, the extraction solution 46a in the first accommodating space 112 is continuously injected into the sample injecting space 120 to generate a genetic sample ( 46 in FIG. 12 is extracted (step S140).

Referring to FIGS. 5, 6, and 12, 13, 18, and 19, the sample 46 is separated from the gene capture member 130 by the extraction solution 46a and extracted. Temporarily store in step 158 (step S150).

Specifically, the slide valve assembly 200 is pushed in the right direction to move the third air inlet 281 onto the first air inlet 112a of the first accommodating space 112, and the second extraction connector 283. And the other end of the first to the sample injection space 120, and the ends of the second extraction connecting portion 285 to the first terminal 152a of the first conduit 152 and the sample storage tube of the sample storage tube 158 Move to terminal 158a.

When a negative pressure is applied to the air valve 165, the negative pressure is collected through the third conduit 156, the sample storage tube 158, the second extraction connector 285, and the lower portion of the sample injection space 120. And applied to the genetic sample 46 isolated from 130).

Gene sample 46 is moved into the sample reservoir 158 by the negative pressure.

5, 6, and 18 to 20, the negative pressure applied to the gene sample 46 is subsequently removed (step S160).

Since the gene capture member 130 includes a porous medium, a very high level of negative pressure is applied to the air valve 165 to move the gene sample 46 to the sample storage tube 158. When excessive negative pressure is applied to the air valve 165, it becomes difficult to discharge the genetic sample 46 in a later step, so that the external air 59 flows in the opposite direction to the direction in which the genetic sample 46 has moved, thereby reducing the negative pressure. Remove That is, when the sample storage tube terminal 158a is connected to the outside in the state in which the negative pressure is present in the third conduit 156 in the state in which the gene sample 46 fills the sample storage tube 158, the external air due to the negative pressure Is rapidly introduced into the sample storage pipe 158 through the sample storage pipe terminal 158a. When the external air is rapidly introduced into the sample storage tube 158, the genetic sample 46 exits toward the air valve 165 along the third conduit 156. In this embodiment, the negative pressure in the sample storage tube 158 is removed to prevent the genetic sample 46 from being lost by escaping toward the air valve 165.

Specifically, the slide valve assembly 200 is pushed in the right direction to connect the negative pressure removing hole 267 to the negative pressure removing tube terminal 159a of the negative pressure removing conduit 159.

Since the negative pressure removing hole 267 is connected to the outside, the outside air 57 is introduced through the negative pressure removing hole 267. The introduced external air 57 is introduced into the air valve 165 and the sample storage pipe 158 through the negative pressure removing conduit 159 and the third conduit 156. The negative pressure applied to the gene sample 46 in the previous step is removed by the external air 57 introduced into the sample storage tube 158.

5, 6, and 16 to 19, the genetic sample 46 temporarily stored in the sample storage tube 158 is finally discharged (step S170).

According to the present embodiment as described above, the slide valve assembly 400 includes a negative pressure removing hole 267 spaced apart from the sample extraction port 270 and the negative pressure removing conduit 159 is based on the air valve 165. Disposed on the opposite side of the third storage space 116, and the residue disposed in the third storage space 116 moves toward the sample storage tube 158 to contaminate the genetic sample 46 in the process of removing the negative pressure. Is prevented.

According to the embodiment of the present invention as described above, it is possible to simply capture the gene using the gene capture member, there is no need for a centrifuge. In addition, the process of gene capture, washing, etc. can be performed in an integrated sample collection device, and a separate reagent container is not necessary, so that a genetic sample can be immediately taken in a harsh environment such as a site.

In addition, since the extraction solution and the washing solution are automatically flowed out by the slide valve assembly and air pressure (or negative pressure), the arrangement of the first storage space and the second storage space is not limited. Therefore, contamination of the extraction solution and the washer solution is prevented, and the design of the sample extraction device is free.

In addition, the slide valve assembly includes a negative pressure removing hole disposed to be spaced apart from the sample extraction port and the negative pressure removing conduit is disposed on the opposite side of the third storage space relative to the air valve, and disposed in the third storage space in the process of removing the negative pressure This residue is prevented from moving towards the sample reservoir and contaminating the genetic sample.

In addition, the time taken to collect the genetic sample is reduced, and even without special technology, anyone can easily collect the genetic sample and the cost is reduced.

The present invention has an industrial applicability that can be used for research, disaster prevention, medical, livestock, pet treatment, etc. as a device for performing tests by amplifying genetic material, blood test apparatus, disease test apparatus, and the like.

44a: washer solution 42, 44: residue
46a: extract solution 46: genetic sample
50: guide bar 52, 56, 58: sound pressure
53, 57, 59: air 60: guide rail
100: main body 112: first storage space
112a: first air inlet 112b: extraction solution outlet
114: second storage space 114a: second air inlet
114b: washer solution outlet 116: third storage space
120: sample injection space 130: gene capture member
140: rotary valve 152: first conduit
152a: first terminal 154: second conduit
154a: second terminal 156: third conduit
158: sample storage tube 158a: sample storage tube terminal
159: negative pressure removing conduit 159a: negative pressure removing pipe terminal
165: air valve 200: slide valve assembly
201: Lubricant 220: Sample inlet
225: first sample injection connection 241: first air inlet
243: first washer solution connection part 245: second washer solution connection part
261: second air inlet 263: first extraction solution connection
265: second extraction solution connecting portion 267: negative pressure removing hole
270: sample extraction port 272: sample extraction connection conduit
281: third air inlet 283: first extraction connection
285: second extraction connection

Claims (6)

A sample injection space into which a raw sample is injected, a sample storage tube for temporarily storing a gene sample in the raw sample, a storage space in which the residue from which the genetic sample is extracted from the raw sample is accommodated, the sample injection space, A main body including the sample storage tube and a plurality of conduits connected to the receiving space;
A gene trap member disposed in the sample injection space to capture only the gene sample from the raw sample and separate from the gene sample when the gene sample is mixed with an extraction solution; And
It is coupled to the main body so as to be slidable, is placed in close contact with the main body, the residue of the raw sample disposed therein, the washing solution, and the extraction solution is in close contact with each other so as not to leak out, the main body It includes a slide valve assembly formed on the opposite surface and including a plurality of connecting portions for controlling the connection of the conduits by sliding and a sample extraction port for discharging the genetic sample temporarily stored in the sample storage tube,
The genetic sample is separated from the surface of the gene capture member when mixed with the extract solution,
The slide valve assembly includes a plurality of connection portions exposed on the rear surface and having a recess shape through which the genetic sample passes.
According to the horizontal movement of the slide valve assembly, both ends of the connection portion sample collection device, characterized in that to sequentially connect the terminals of two different conduits of the conduits. The sample collection device according to claim 1, wherein the main body further comprises a first accommodating space in which the extraction solution is stored, and a second accommodating space in which the washer solution is stored. The sample collection device of claim 1, further comprising an air valve coupled to the main body to apply negative pressure to the storage space and the sample storage tube or to inject air. A sample injection space into which a raw sample is injected, a sample storage tube for temporarily storing a gene sample in the raw sample, a storage space in which the residue from which the genetic sample is extracted from the raw sample is accommodated, and the sample injection space and the sample A main body including a storage tube, and a plurality of conduits connected to the receiving space, and disposed in the sample injection space to capture only the gene sample from the raw sample and when the gene sample is mixed with an extraction solution, The gene capture member is separated, and coupled to the main body so as to be slidable, and closely adhered to the main body so that the residue, the washer solution, and the extraction solution of the raw sample disposed therein may not be leaked to the outside. And the conduits formed on the surface facing the main body by sliding. A slide valve assembly including a plurality of connections for controlling a connection relationship and a sample extraction port for discharging a gene sample temporarily stored in the sample storage tube, and coupled to the main body to the storage space and the sample storage tube; In the sampling method using a sampling device including an air valve connected,
Injecting the raw sample into the sample injection space;
The first terminal of the first conduit connected to the sample injection space and the second terminal of the second conduit connected to the accommodation space are moved through the first connection part of the slide valve assembly by moving the slide valve assembly horizontally. Storing the residue in the receiving space and capturing the genetic sample into the gene trapping member;
Accommodating a negative pressure to the air valve while the washing solution is applied to a sample inlet formed through the slide valve assembly in a vertical direction to accommodate the rest of the residue in the storage space;
The first terminal of the first conduit connected to the sample injection space by moving the slide valve assembly in the horizontal direction and the sample storage tube terminal of the sample storage tube are connected through the sample extraction connection conduit of the slide valve assembly. The sample is formed on the main body in a state in which the extraction solution is applied to the sample inlet, and a negative pressure is applied to an air valve disposed between the accommodation space and the sample storage tube to transfer the gene sample to the sample. Temporarily storing in a reservoir;
Controlling the slide valve assembly to remove the negative pressure applied to the air valve; And
And controlling the slide valve assembly to connect the sample storage tube and the sample extraction port to discharge the gene sample. The method of claim 4, wherein removing the negative pressure comprises introducing external air through the sample extraction port. The method of claim 4, wherein the step of temporarily storing the genetic sample in the sample storage tube,
Applying the negative pressure from the upper portion of the sample injection space;
Manually introducing the extract solution from the upper portion of the sample injection space by using the negative pressure; And
And using the extracting solution to wet the gene trapping member disposed below the sample injection space from an upper surface thereof.

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