KR102044344B1 - Microfludic device - Google Patents

Abstract

One embodiment of the present invention is a microfluidic device, comprising: a fluid receiving portion; Fluid reaction unit; And at least one flow passage connected to at least one of the fluid receiving portion and the fluid reaction portion, wherein at least one of the flow passages includes a fluid accelerator including a hydrophilic line and a plurality of hydrophobic island patterns spaced apart from each other. Provided is a microfluidic device including at least one of the reduction units.

Description

Microfluidic Device {MICROFLUDIC DEVICE}

The present invention relates to a microfluidic device.

A chemical laboratory unit, where specialists use precision instruments and equipment to handle reactions that require long, repeated operations, quantitatively isolates, transports, synthesizes, and evicts microliters of liquid samples on a single palm-sized substrate. The device includes a chemical reactor that is scaled down on a single chip so that most chemical reactions can be processed accurately, quickly and programmatically, as well as to detect and inspect the reaction product. "Lab-on-a-chip", micrototal analysis system (m-TAS), "microfluidic chip" or "microfluidic chip"

Microfluidic chip not only obtains various compounds based on inorganic chemical reactions, but also analyzes biological cell reactants through cellular reactions, analyzes DNA on the basis of immunoenzyme antigen-antibody reactions, and diagnoses and treats diseases. These are chemical, biological, and clinical reactors of fluid chemistry that are expected to be useful.

Since the last 20 years, many kinds of microfluidic chips have been developed, and pneumatic, ultrasonic, interfacial tension, voltage driven, magnetically driven, and electrofluidic fluid chips are used depending on the driving force. It is classified into glass chips, plastic chips including PDMS, silicon chips, PCB chips, and paper chips according to the substrate. In recent years, voltage-driven formulas have become central to R & D and commercialized products due to the simplicity of the structure and elimination of complicated pipes carrying continuous fluids and pneumatic pumps.

However, in the case of the microfluidic chip using voltage driving, an electrode is formed on the microfluidic chip in order to control the movement of the fluid on the microfluidic chip, and a separate voltage supply device for supplying a voltage to the electrode is required. . Therefore, the manufacturing of the microfluidic chip is somewhat complicated, and there is a inconvenience in that the voltage must be adjusted to control the movement of the fluid.

Therefore, there is a need for a microfluidic device capable of freely controlling the moving speed of the fluid on the microfluidic chip in a simple manner and performing various reactions.

The present invention is to provide a microfluidic device that can control the moving speed of the fluid.

One embodiment of the present invention is a microfluidic device, comprising: a fluid receiving portion; Fluid reaction unit; And at least one flow passage connected to at least one of the fluid receiving portion and the fluid reaction portion, wherein at least one of the flow passages includes a fluid accelerator including a hydrophilic line and a plurality of hydrophobic island patterns spaced apart from each other. Provided is a microfluidic device including at least one of the reduction units.

According to one embodiment of the present invention, the microfluidic device includes a fluid accelerator and / or a fluid decelerator, so that the speed of movement of the fluid on the microfluidic device can be easily controlled.

According to one embodiment of the present invention, by controlling the shape, line width, length, etc. of the hydrophilic line, it is possible to control the fluid movement speed in the fluid accelerator variously.

According to an embodiment of the present invention, by controlling the number of hydrophobic island patterns, the separation distance, the diameter of the pattern, etc., it is possible to variously control the fluid movement speed in the fluid deceleration unit.

According to one embodiment of the present invention, the moving speed of fluids moving on one microfluidic device may be about 30 times different.

According to one embodiment of the present invention, the microfluidic device has an advantage of being able to perform various chemical reactions simply by using only a small amount of reactants or samples.

According to one embodiment of the present invention, the paper, film, glass, etc., which can be easily obtained from the surroundings, can be used as a substrate, and thus, the microfluidic device can be easily manufactured at low cost.

1 is a view showing a fluid movement speed according to the width of the flow path according to an embodiment of the present invention.
2A and 2B are views illustrating a microfluidic device including a fluid receiving part, a fluid reaction part, and a flow path according to an embodiment of the present invention.
3A and 3B are views illustrating a microfluidic device including two or more fluid receiving portions and a fluid reaction portion according to one embodiment of the present invention.
4 is a view showing a flow path including a fluid accelerator according to an embodiment of the present invention.
5 is a view showing a flow path including a fluid deceleration part according to an embodiment of the present invention.
Figure 6a is a view showing a plurality of flow paths are all connected to the fluid receiving portion according to an embodiment of the present invention, Figure 6b is a plurality of flow paths are each independently connected to the fluid receiving portion according to an embodiment of the present invention The figure shown.
7 is a view schematically showing the configuration of a microfluidic device according to an embodiment of the present invention.
8 is a view showing a moving speed of the fluid according to the width of the hydrophilic line provided on the substrate according to an embodiment of the present invention.
FIG. 9 is a diagram illustrating a moving speed of a fluid according to distribution of a hydrophobic island pattern provided on a substrate according to an embodiment of the present invention.
FIG. 10 illustrates a fluid movement speed in a microfluidic device with a hydrophilic line, a microfluidic device with a hydrophobic island pattern, and a microfluidic device without a hydrophilic line and a hydrophobic island pattern according to an embodiment of the present invention. Drawing.
11A illustrates a microfluidic device in which one fluid receiving part and four reaction parts are formed according to an embodiment of the present invention, and FIG. 11B illustrates that glucose is detected on the microfluidic device according to an embodiment of the present invention. 11C to 11D are diagrams showing the results of glucose detection reaction in the microfluidic device according to one embodiment of the present invention.

When any part of the specification is to "include" any component, this means that it may further include other components, except to exclude other components unless otherwise stated.

Also, when a member is located "on" another member, this includes not only when one member is in contact with another member but also when another member exists between the two members.

In addition, when a part of the specification is "connected" to another part, this includes not only "directly connected", but also "connected with other elements in the middle".

Hereinafter, this specification is demonstrated in detail.

One embodiment of the present invention is a microfluidic device, comprising: a fluid receiving portion; Fluid reaction unit; And at least one flow passage connected to at least one of the fluid receiving portion and the fluid reaction portion, wherein at least one of the flow passages includes a fluid accelerator including a hydrophilic line and a plurality of hydrophobic island patterns spaced apart from each other. Provided is a microfluidic device including at least one of the reduction units.

According to an embodiment of the present invention, the flow path of the microfluidic device includes a fluid accelerator and / or a fluid decelerator, so that the moving speed of the fluid on the microfluidic device can be easily controlled.

In addition, according to an embodiment of the present invention, the fluid fluid device may not be provided with a separate power supply for moving the fluid on the microfluidic device. Specifically, the microfluidic device may adjust the moving speed of the fluid on the microfluidic device without a separate power supply.

Therefore, according to an embodiment of the present invention, the microfluidic device does not need to have a separate power supply device, and thus, the manufacturing cost of the microfluidic device can be effectively reduced and it is easy to carry. In addition, as a plurality of flow paths having different moving speeds of the fluid are provided in the microfluidic device, various chemical experiments, detection, and inspection may be performed on the microfluidic device.

According to an embodiment of the present invention, the fluid may be supplied to the body fluid device through the fluid receiving portion. In addition, the fluid reaction part may perform a chemical reaction of the fluid supplied to the fluid receiving part. The microfluidic device may include a fluid reaction part to perform various chemical reactions on the microfluidic device. The fluid reaction part may include a sample, and the type of the sample included in the fluid reaction part may be different according to a chemical reaction of the fluid performed in the fluid reaction part. In detail, when detecting a specific substance included in the fluid, the fluid reaction unit may be a sensor unit, and the fluid reaction unit may include a sample capable of detecting a specific substance included in the fluid.

The fluid may undergo synthesis reaction, oxidation reaction, reduction reaction, decomposition reaction, substitution reaction, precipitation reaction, detection reaction or acid-base reaction on the microfluidic device. Specifically, as a chemical reaction that can be performed on the microfluidic device, a chemical synthesis reaction for synthesizing a polymer or DNA, a surface modification reaction for modifying the surface of graphene or clay, and a quantum dot Alternatively, an ionic reduction reaction for reducing silver nanoparticles, a glucose detection reaction, or the like may be performed on the microfluidic device. However, the type of the chemical reaction described above is merely an example for description and does not limit the type of chemical reaction.

Therefore, according to one embodiment of the present invention, the microfluidic device has the advantage of being able to easily perform various chemical reactions with only a small amount of reactants or samples.

1 is a view showing a fluid movement speed according to the width of the flow path according to an embodiment of the present invention. Specifically, FIG. 1 is a view illustrating a fluid moving speed according to a width of a flow path in a flow path without a fluid accelerator and a fluid speed reducer.

According to an embodiment of the present invention, the width of the flow path may be 50 μm or more and 10,000 μm or less. Specifically, the width of the flow path may be 150 μm or more and 4,000 μm or less, 500 μm or more and 3,000 μm or less.

The flow path is a micro flow path having a micro size width, and when the fluid is supplied to the fluid receiving portion connected to the flow path, the fluid accommodated in the fluid receiving portion by capillary action may move in the flow path. Specifically, a capillary force is applied between the wall of the flow path and the fluid, so that the fluid may move in the flow path.

Referring to FIG. 1, when the width of the flow path is greater than or equal to 160 μm and less than or equal to 1,000 μm, the moving speed of the fluid is rapidly increased, and the moving speed of the fluid is slightly increased between 1,000 μm and 2,500 μm or less. Therefore, according to an embodiment of the present invention, by controlling the width of the flow path, it is possible to control the fluid movement speed in the flow path.

According to an embodiment of the present invention, the flow path may connect the fluid receiving part and the fluid reaction part. In detail, the flow path may connect one fluid receiving part and one fluid reaction part, connect one fluid receiving part and a plurality of fluid reaction parts, or connect a plurality of fluid receiving parts and one fluid reaction part. Specifically, when one fluid receiving part and one fluid reaction part are connected by the flow path, and the one fluid reaction part and the other fluid reaction part are connected by the other flow path, the fluid contained in the fluid receiving part is The first reaction may proceed in one fluid reaction part, and then the second reaction may proceed by moving to the other fluid reaction part.

Therefore, according to an embodiment of the present invention, by connecting the fluid receiving portion and the fluid reaction portion provided on the microfluidic device in various ways using a flow path, complex reactions such as a step reaction and a multiple reaction can be performed. .

According to an embodiment of the present invention, the fluid accelerator and the fluid decelerator may be provided in part or all of the flow path. Specifically, the fluid acceleration part or the fluid deceleration part may be provided in all of the flow paths, the fluid acceleration part may be provided in a part of the flow path, and the fluid deceleration part may be provided in the other part. That is, the flow path may include a fluid accelerator, a fluid reducer, or a fluid accelerator and a fluid reducer together.

2A to 2C are views illustrating a microfluidic device including a fluid receiving part, a fluid reaction part, and a flow path according to an embodiment of the present invention. In detail, FIG. 2A illustrates a fluid accelerator including a fluid receiving part 100 and a fluid reaction part 200 connected through one flow path 300, and including a hydrophilic line 410 in the flow path 300. A diagram showing a microfluidic device 400 is provided. FIG. 2B illustrates a fluid deceleration part including a fluid receiving part 100 and a fluid reaction part 200 connected through one flow path 300, wherein the flow path 300 includes a plurality of island patterns 510. 500 is a view showing a microfluidic device provided. In addition, in FIG. 2C, the fluid receiving part 100 and the fluid reaction part 200 are connected through one flow path 300, and the flow acceleration part 400 includes a hydrophilic line 410 in the flow path 300. ) And a microfluidic device including a fluid deceleration part 500 including a plurality of island patterns 510.

According to an embodiment of the present invention, the microfluidic device may include two or more flow paths, and the flow paths may have different fluid movement speeds. The microfluidic device may include two or more of the fluid receiving part, the fluid reaction part, and the flow path. Specifically, one fluid reaction part may be connected to two fluid receiving parts by two flow paths, respectively. In addition, two fluid reaction units may be connected to each other by one flow path, and each of the two fluid reaction parts may be connected to one fluid receiving part by two flow paths.

According to an embodiment of the present invention, when the microfluidic device includes a plurality of flow paths, the plurality of flow paths may include flow paths in which the fluid accelerator or the fluid deceleration part is not provided. That is, the microfluidic device may include a flow path provided with a fluid accelerator, a flow path provided with a fluid reducer, a flow path not provided with the fluid accelerator or the fluid reducer.

3A and 3B are views illustrating a microfluidic device including two or more fluid receiving portions and a fluid reaction portion according to one embodiment of the present invention. In detail, FIG. 3A illustrates that one fluid receiving part 110 and the fluid reaction part 200 are connected through the flow path 310, and the other fluid receiving part 120 and the fluid reaction part 200 are flow paths. A diagram showing a microfluidic device connected via 320. In addition, in FIG. 3B, one fluid receiving part 100 is connected to two fluid reaction parts 210 and 220 by two flow paths 310 and 320, and the two fluids are connected by one flow path 330. A diagram showing microfluidic devices in which reactors 210 and 220 are connected to each other.

According to an embodiment of the present invention, the fluid accelerator may mean an area including the hydrophilic line in the flow path, and the fluid deceleration part may mean an area including the hydrophobic island pattern in the flow path.

According to an embodiment of the present invention, the fluid movement speed may be increased in the fluid accelerator provided on the microfluidic device. That is, the fluid moving in the flow path may be increased in the fluid acceleration portion. The hydrophilic line included in the fluid accelerator includes a hydrophilic material, and when the fluid is located on the hydrophilic line, the contact angle of the fluid may be reduced. Specifically, the contact angle of the fluid on the paper is about 46 °, but the contact angle of the fluid on the surface coated with the hydrophilic silver nanoparticles is reduced to about 18 ° so that the fluid can move better on the surface coated with silver nanoparticles . That is, as the contact angle of the fluid is reduced on the fluid accelerator provided with the hydrophilic line, the moving speed of the fluid in the fluid accelerator may be increased.

On the other hand, the fluid movement speed in the fluid deceleration unit provided on the microfluidic device can be reduced. That is, the fluid moving in the flow path may be reduced in the moving speed in the fluid deceleration portion. Since the hydrophobic island pattern included in the fluid deceleration part includes a hydrophobic material, when the fluid is positioned on the hydrophobic island pattern, the contact angle of the fluid may be increased. Specifically, the contact angle of the fluid on the paper is about 46 °, but as the contact angle of the fluid on the surface coated with the hydrophobic polytetrafluoroethylene increases to about 120 °, the fluid is coated on the polytetrafluoroethylene Mobility can be reduced. That is, as the contact angle of the fluid on the fluid deceleration portion provided with the hydrophobic island pattern is increased, the moving speed of the fluid in the fluid deceleration portion may be reduced.

According to one embodiment of the invention, the hydrophilic line may comprise at least one metal selected from the group consisting of gold, platinum, silver, aluminum and copper. Specifically, the hydrophilic line may include one or more metal nanoparticles selected from the group consisting of gold nanoparticles, platinum nanoparticles, silver nanoparticles, aluminum nanoparticles, and copper nanoparticles.

The portion of the fluid accelerator including the hydrophilic line including one or more metals selected from the group consisting of gold, platinum, silver, aluminum, and copper may have a larger surface energy than a portion without the hydrophilic line. . The contact angle of the fluid on the fluid accelerator with large surface energy can be reduced, thereby increasing the speed of movement of the fluid in the fluid accelerator.

According to one embodiment of the present invention, the diameter of the metal nanoparticles may be 10 nm or more and 1,000 nm or less. By using the metal nanoparticles having a diameter of 10 nm or more and 1,000 nm or less, it is possible to increase the surface energy of the fluid accelerator portion having the hydrophilic line.

According to one embodiment of the invention, the hydrophobic island pattern comprises at least one selected from the group consisting of fluorine-containing polymer, polystyrene, ethylene vinyl acetate copolymer, polycarbonate, polymethyl (meth) acrylate and carbon nanotubes. can do. Specifically, the fluorine-containing polymer may be polytetrafluoroethylene, called Teflon, and the carbon nanotubes may be single-walled carbon nanotubes, double-walled carbon nanotubes, multi-walled carbon nanotubes, or multiple carbon nanotubes. have.

The fluid reduction portion portion with the hydrophobic island pattern comprising at least one selected from the group consisting of the fluorine-containing polymer, polystyrene, ethylene vinyl acetate copolymer, polycarbonate, polymethyl methacrylate and carbon nanotubes The surface energy may be lower than that of the portion without the hydrophobic island pattern. The contact angle of the fluid on the fluid deceleration portion with small surface energy can be increased, whereby the speed of movement of the fluid in the fluid deceleration portion can be reduced.

According to an embodiment of the present invention, the flow path including the fluid accelerator essentially includes the hydrophilic line, and may optionally include the hydrophobic island pattern. In addition, the flow path including the fluid reduction portion may include the hydrophobic island pattern, and may optionally include the hydrophilic line. As the hydrophobic island pattern is selectively included in the flow path including the fluid accelerator, or the hydrophilic line is selectively included in the flow path including the fluid deceleration part, the moving speed of the fluid moving in the flow path can be more precisely controlled. Can be.

According to an embodiment of the present invention, two or more flow paths included in the microfluidic device may have different fluid movement speeds. Referring to FIG. 3A, the fluid movement speed of the oil passage 310 including the fluid accelerator 400 including the hydrophilic line 410 may be greater than the fluid movement speed of the oil passage 320 without the fluid accelerator. . That is, the moving speed of the fluid in the flow path 310 with the fluid accelerator 400 may be faster than the moving speed of the fluid in the flow path 320 without the fluid accelerator.

In addition, referring to FIG. 3B, the fluid movement speed of the flow path 310 provided with the fluid deceleration part 500 including the hydrophobic island pattern 510 is fluid in the flow path 330 including the fluid accelerator 400. It may be less than the movement speed. That is, the moving speed of the fluid in the flow path 310 with the fluid deceleration part 500 may be slower than the moving speed of the fluid in the flow path 330 with the fluid accelerator 400.

Therefore, according to one embodiment of the present invention, by setting various positions provided with the fluid accelerator and the fluid deceleration portion included in the flow path, it is possible to more effectively control the moving speed of the fluid moving in the flow path.

According to an embodiment of the present invention, the fluid accelerator may adjust the moving speed of the fluid by adjusting at least one of the length of the hydrophilic line and the line width. As the line width of the hydrophilic line increases, the moving speed of the movement of the fluid in the fluid accelerator may increase. As the width of the hydrophilic line increases, the area in which the hydrophilic line is in fluid contact with the hydrophilic line may increase. As a result, the moving speed of the fluid in the fluid accelerator including the hydrophilic line may be increased. In addition, as the length of the hydrophilic line is longer, the section of the fluid accelerator in which the moving speed of the fluid is accelerated is increased, so that the fluid can be moved faster to a desired point. Specifically, as the fluid accelerator including the hydrophilic line is provided over the entire length direction of the flow path, the fluid movement speed in the flow path can be effectively increased.

According to an embodiment of the present invention, the hydrophilic line means a line continuously connected from one point to another point, and may have a shape of straight line, sawtooth pattern, curve or wave pattern.

4 is a view showing a flow path including a fluid accelerator according to an embodiment of the present invention. Specifically, FIG. 4 is a view illustrating a flow path 300 having a fluid accelerator 400 including a hydrophilic line 410.

According to an embodiment of the present invention, the fluid accelerator may include a plurality of hydrophilic lines. Referring to FIG. 4, the fluid accelerator 400 may include a plurality of hydrophilic lines 410 having the same line width. In addition, the fluid accelerator may include a plurality of hydrophilic lines having different line widths, and may include a plurality of hydrophilic lines having different lengths. In addition, the line width of the hydrophilic line may be changed along the length direction of the flow path. Specifically, the hydrophilic line may have a shape in which the line width of the hydrophilic line increases or decreases along the length direction of the flow path.

In addition, the fluid accelerator may include a hydrophilic line and a hydrophilic dot. Specifically, the hydrophilic line and the hydrophilic dot may be provided alternately along the longitudinal direction of the flow path.

Therefore, according to an embodiment of the present invention, by controlling the shape, line width, length, etc. of the hydrophilic line, it is possible to variously control the moving speed of the fluid in the fluid accelerator.

According to an embodiment of the present invention, the ratio of the width of the flow path and the line width of the hydrophilic line may be 1: 0.005 to 1: 1. Specifically, the ratio of the width of the flow path and the line width of the hydrophilic line may be 1: 0.05 to 1: 0.95. By adjusting the ratio of the width of the flow path and the line width of the hydrophilic line, it is possible to control the area of the portion in which the hydrophilic line is not provided in the fluid accelerator. Specifically, by reducing the area of the portion that is not provided with the hydrophilic line on the fluid accelerator, it is possible to further increase the moving speed of the fluid in the fluid accelerator.

According to one embodiment of the invention, the hydrophobic island pattern may be a shape consisting of a circle, a polygon or a curve. Specifically, the hydrophobic island pattern may have an elliptical shape, a hemispherical shape, or the like in addition to a circular shape. In addition, the hydrophobic island pattern may have a shape in which at least one side of the polygon is curved in addition to a polygon such as a triangle, a square, a pentagon, a hexagon, and the like. However, the shape of the hydrophobic island pattern described above is for illustrative purposes only and does not limit the shape of the hydrophobic island pattern.

According to an embodiment of the present invention, the fluid deceleration unit may adjust the moving speed of the fluid by adjusting at least one of the number of the hydrophobic island patterns, the separation distance, and the diameter of the island pattern.

5 is a view showing a flow path including a fluid deceleration part according to an embodiment of the present invention. In detail, FIG. 5 is a view illustrating a flow path 300 including the fluid deceleration part 400 including the hydrophobic island pattern 410.

According to an embodiment of the present invention, when the hydrophobic island pattern included in the fluid deceleration part is circular, the fluid deceleration part may include a plurality of hydrophobic island patterns having the same diameter, and a plurality of hydrophobic islands having different diameters from each other. It can include a pattern. In addition, referring to FIG. 5, the distance between the plurality of hydrophobic island patterns 410 included in the island pattern is controlled to control the distribution of the hydrophobic island pattern 410 per unit area of the fluid reduction unit. can do. In addition, by controlling the number of hydrophobic island patterns included in the fluid deceleration portion, it is possible to control the fluid movement speed in the fluid deceleration portion. Specifically, as the separation distance and the number of the plurality of hydrophobic island patterns are reduced, the fluid movement speed in the fluid deceleration portion may be reduced.

Therefore, according to one embodiment of the present invention, by controlling the number of hydrophobic island patterns, the separation distance, the diameter of the pattern, etc., it is possible to variously control the fluid movement speed in the fluid deceleration unit.

According to an embodiment of the present invention, the ratio of the width of the flow path and the diameter of the hydrophobic island pattern may be 1: 0.05 to 1: 0.2. Specifically, when the hydrophobic island pattern is circular, the ratio of the width of the flow path and the diameter of the hydrophobic island pattern may be 1: 0.05 to 1: 0.2. On the other hand, when the hydrophobic island pattern is not circular, the ratio of the long width having the longest length among the width of the flow path and the width of the hydrophobic island pattern in the direction perpendicular to the length direction of the flow path is 1: 0.05 to 1: 0.2 Can be.

By adjusting the ratio of the width of the flow path and the diameter of the hydrophobic island pattern to 1: 0.05 to 1: 0.2, it is possible to prevent the fluid from passing the portion where the hydrophobic island pattern is formed, and on the fluid deceleration part It is possible to effectively reduce the fluid movement speed of the.

According to an embodiment of the present invention, the fluid accelerator may further include a plurality of hydrophobic island patterns, and the fluid decelerator may further include a hydrophilic line. Specifically, when the hydrophilic line included in the fluid accelerator is provided along the length direction of the flow path, the hydrophobic island pattern may be provided on both sides of the hydrophilic line along the width direction of the flow path. In addition, when the plurality of hydrophobic island patterns included in the fluid deceleration unit are provided to be spaced apart from each other, the hydrophilic line may be provided along the length direction of the flow path between the plurality of island patterns.

As a plurality of hydrophobic island patterns are further included in the fluid accelerator, or a hydrophilic line is further included in the fluid decelerator, the moving speed of the fluid in the fluid accelerator and the fluid decelerator may be more precisely controlled.

According to an embodiment of the present invention, the fluid receiving portion may be all connected to the two or more flow paths, or may be connected to each of the flow paths independently. Specifically, one flow path connected to one fluid receiving part may be divided into several branches and connected to the plurality of fluid reaction parts. In addition, each of the plurality of fluid reaction units may be independently connected by one fluid receiving part and a plurality of flow paths.

Figure 6a is a view showing a plurality of flow paths are all connected to the fluid receiving portion according to an embodiment of the present invention, Figure 6b is a plurality of flow paths are each independently connected to the fluid receiving portion according to an embodiment of the present invention The figure shown. In detail, FIG. 6A is a view showing that the fluid paths 310 and 320 connected to the two fluid reaction parts 210 and 220 are connected to the one fluid receiving part 100 in a state where they are combined. In addition, FIG. 6B is a view showing that flow paths 310 and 320 respectively connected to two fluid reaction parts 210 and 220 are independently connected to one fluid receiving part 100.

According to an embodiment of the present invention, the fluid reaction part may be all connected to the two or more flow paths, or may be independently connected to each of the flow paths. Specifically, one flow path connected to one fluid reaction part may be divided into several branches and connected to the plurality of fluid receiving parts. In addition, the plurality of fluid receiving parts may be independently connected to one fluid reaction part and a plurality of flow paths, respectively.

Therefore, according to an embodiment of the present invention, the microfluidic device may include various types of flow paths, and thus there is an advantage in that a complicated chemical reaction can be easily performed.

According to an embodiment of the present invention, by adjusting the line width, number, shape, length, etc. of the hydrophilic line included in the fluid accelerator, the portion of the fluid acceleration portion in the fluid acceleration portion is not provided with the portion It can be increased by about 15 times. In addition, by adjusting the shape, area, number, separation distance, diameter, etc. of the plurality of hydrophobic island patterns, the fluid movement speed in the fluid deceleration portion is reduced by about twice as compared to the portion not provided with the hydrophobic island pattern. You can.

Therefore, according to one embodiment of the present invention, the moving speed of fluids moving on one microfluidic device may be about 30 times different. Specifically, in the case of one flow path in which the fluid accelerator is formed and the other flow path in which the fluid reducer is formed, the speed of fluid movement in the fluid accelerator is increased by about 15 times, and the speed of fluid movement in the fluid reducer is reduced by about 2 times. In this way, the difference in the moving speeds of the fluids on one microfluidic device can be controlled to be about 30 times.

Therefore, according to one embodiment of the present invention, by controlling the difference in the movement speed of the fluid on the microfluidic device to about 30 times, the microfluidic device can perform a chemical reaction more precisely, various chemical reactions Can be easily proceeded.

According to one embodiment of the invention, the microfluidic device is a cellulose substrate coated with a water repellent; Polymer substrates; It may be provided on a substrate selected from the group consisting of silicon-based glass.

Specifically, the cellulose substrate may be paper, and paper coated with silica nanoparticles may be used as the substrate. Moreover, a polyethylene film, a polypropylene film, a polystyrene film, a polyethylene terephthalate film, a polyvinyl chloride film, etc. can be used as a polymer base material. In addition, the material used as the water repellent may use a conventional material used when water repellent coating the film in the art. Specifically, as the water repellent, one containing at least one selected from the group consisting of silica nanoparticles, alumina, potassium carbonate and plastic clay may be used.

By using the paper coated with the silica nanoparticles, polyethylene terephthalate film, glass or the like as a substrate, even when the fluid moves in the flow path formed on the substrate can be prevented from being absorbed by the substrate.

According to an embodiment of the present invention, the hydrophilic line and the hydrophobic island pattern may be provided on the microfluidic device by an inkjet printing process, a silk screen process or a dispensing process. The printer used in the inkjet printing process may use a conventional printer for printing ink in the art. Specifically, a printer using a piezo electric head may be used as a printer used in the inkjet printing process.

According to an embodiment of the present invention, an ink including a hydrophilic material and an ink including a hydrophobic material may be prepared, and the ink may be printed or applied to form hydrophilic lines and hydrophobic island patterns on the microfluidic device. . Specifically, the ink may be printed by an inkjet printer, and the ink-filled pen may be used to draw hydrophilic lines and hydrophobic island patterns.

Therefore, according to an embodiment of the present invention, a hydrophilic line and a hydrophobic island pattern may be provided on the microfluidic device in a simple manner, thereby easily manufacturing the microfluidic device.

According to an embodiment of the present invention, a drawing of a hydrophilic line or hydrophobic island pattern of a desired shape may be created using a program such as Photoshop or CAD, and the hydrophilic line or hydrophobic island pattern may be printed using the prepared drawing. .

According to one embodiment of the invention, the microfluidic device can be reused. Specifically, the microfluidic device may be reused by removing the fluid remaining on the microfluidic device after performing the chemical reaction on the microfluidic device and dipping the microfluidic device in the silicone oil.

According to an embodiment of the present invention, the microfluidic device may have a laminated structure. Specifically, the microfluidic film having the fluid accommodating part, the fluid reaction part and the flow path may be stacked on the base film provided with the hydrophilic line or the plurality of hydrophobic island patterns. The microfluidic film may be stacked on the base film such that a hydrophilic line or a plurality of hydrophobic island patterns provided on the base material are positioned in the flow path of the microfluidic film. In addition, the micro-channel film is laminated on the base film, the cover film may be laminated on the micro-channel film. As the cover film is laminated on the micro channel film, impurities such as dust may be prevented from entering the fluid moving in the flow path. The cover film may be provided with one or more holes, and the one or more holes may be provided at a position of the cover film corresponding to a position of the fluid receiving portion or the fluid reaction portion formed in the micro-flow film. In addition, the base film, the micro- euro film, the cover film may be all paper-based film, the surface may be coated with a water repellent.

7 is a view schematically showing the configuration of a microfluidic device according to an embodiment of the present invention. Specifically, FIG. 7 is a microfluidic channel including a base film 600 having a hydrophilic line 410, a fluid receiving part 100, a fluid reaction part 200, and a flow path 300 included in a microfluidic device. FIG. 700 is a view illustrating a cover film 800 provided with a hole at a position corresponding to the position of the fluid receiving part 100 provided in the film 700 and the micro channel film 700. The microfluidic film 700 may be stacked on the base film 600 such that the hydrophilic line 410 is positioned in the flow path 300 provided in the microfluidic film 700. In addition, the cover film 800 is the micro-flow film (so that the fluid receiving portion 100 provided in the micro-flow film 700 and the holes provided in the cover film 800 correspond to each other). 700).

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only for illustrating the present invention in more detail, it will be apparent to those skilled in the art that the scope of the present invention is not limited by these examples in accordance with the gist of the present invention. .

Preparation of Microfluidic Devices

A4 paper (Epson, Inc.), a plastic adhesive film (Printec, Inc.), and an ink containing silver nanoparticles (DGP 40LT-15C, Advance Nano Products, Inc.) were prepared as a substrate. In addition, Poly [4,5-difluoro-2,2-bis (trifluoromethyl) -1,3-dioxole-co-tetrafluoroethylene] (Sigma-Aldrich) and fluorinert solution (Sigma-Aldrich) Teflon ink having a Teflon content of 0.5 wt% was prepared.

A portion of the plastic adhesive film was etched to form a fluid receiving portion, a fluid reaction portion and a flow path, and the plastic adhesive film was attached on A4 paper. Thereafter, the ink containing the silver nanoparticles was printed on the flow path to prepare a microfluidic chip having a hydrophilic line.

In addition, the plastic adhesive film having the fluid receiving portion, the fluid reaction portion, and the flow path was attached onto the A4 paper, and the Teflon ink was printed on the flow path to prepare a microfluidic chip having a plurality of hydrophobic island patterns. .

Microfluidic On the device  Fluid travel velocity measurement

Measurement of the velocity of fluid movement on hydrophilic lines

Eight substrates having a flow path having a width of 3 mm were prepared, and hydrophilic lines having a width of 0.15 mm, 0.22 mm, 0.44 mm, 0.94 mm, 1.31 mm, 1.75 mm, and 2.82 mm were formed in the flow path formed on the substrate, respectively. The ink containing the silver nanoparticles was printed to form the seven microfluidic devices. In addition, one microfluidic device was prepared without the hydrophilic line.

8 is a view showing a fluid movement speed according to the width of the hydrophilic line provided on the flow path according to an embodiment of the present invention. Specifically, Figure 8 is a view showing the measurement of the fluid movement speed on the eight microfluidic devices.

Referring to FIG. 8, it can be seen that as the width of the hydrophilic line increases, the moving speed of the fluid increases. In addition, it can be seen that the fluid moving speed on the microfluidic device with the hydrophilic line having a width of 2.82 mm is increased by about 15 times than the fluid moving speed on the microfluidic device without the hydrophilic line.

Measurement of the velocity of fluid movement on hydrophilic lines

Four substrates having a flow path having a width of 3 mm were prepared, and as shown in FIG. 5, four, six, and eight hydrophobic island patterns were formed in an area of 5 mm length x 3 mm width on the substrate. Ink was printed to prepare three microfluidic devices. In addition, one microfluidic device having no hydrophobic island pattern was prepared.

FIG. 9 is a diagram illustrating a moving speed of a fluid according to a distribution of a hydrophobic island pattern provided on a flow path according to an exemplary embodiment of the present invention. Specifically, Figure 9 is a view showing the measurement of the fluid movement speed on the four microfluidic devices.

Referring to FIG. 9, it can be seen that as the distribution of the hydrophobic island pattern increases in the same area, the moving speed of the fluid decreases. In addition, it can be seen that the fluid movement speed of the microfluidic device in which the six hydrophobic island patterns are formed is reduced by about twice the fluid movement speed on the microfluidic device in which the hydrophobic island pattern is not provided. On the other hand, in the case of the microfluidic device in which eight hydrophobic island patterns are formed, the fluid cannot be moved in the flow path due to the high hydrophobic island pattern distribution.

FIG. 10 illustrates a fluid movement speed in a microfluidic device having a hydrophilic line, a microfluidic device having a hydrophobic island pattern, and a microfluidic device having no hydrophilic line and a hydrophobic island pattern according to an embodiment of the present invention. Drawing. Specifically, FIG. 10 illustrates a microfluidic device having a hydrophilic line having a width of 0.9 mm by printing ink containing the silver nanoparticles on a flow path having a width of 3 mm, on a flow path having a width of 3 mm. The travel distance versus the travel time of the fluid in the microfluidic device with six hydrophobic island patterns for each area of 10 mm length x 3 mm width and the pristine microfluidic device without hydrophilic lines and hydrophobic island patterns The figure shown.

Referring to FIG. 10, it can be seen that the fluid on the microfluidic device with the hydrophilic line has the longest moving distance during the same travel time, and the fluid on the microfluidic device with the hydrophobic island pattern is the other two microfluids. It can be seen that it takes more time to reach the same distance than the fluid on the device.

Microfluidic device Glucose  Detection reaction

FIG. 11A illustrates a microfluidic device in which one fluid receiving part and four fluid reaction parts are formed according to an embodiment of the present invention. Specifically, FIG. 11 is a view illustrating a microfluidic device in which a flow path connected to the fluid receiving part is divided into four flow paths, and each of the four flow paths is connected to the fluid reaction part.

Microfluidic devices capable of detecting glucose were prepared as follows. As shown in FIG. 11, the fluid receiving part 100 may be etched by etching a portion of the plastic adhesive film such that a flow path connected to the fluid receiving part 100 is connected to four fluid reaction parts 210, 220, 230, and 240. The fluid reaction parts 210, 220, 230, and 240 and flow paths were formed. The plastic adhesive film was attached onto the substrate, and as shown in FIG. 11, three flow paths were printed with ink containing silver nanoparticles to prepare a microfluidic device having a hydrophilic line. Referring to FIG. 11, four flow paths on the microfluidic device have the same width, and the flow path connected to the first reaction part 210 is not provided with a hydrophilic line, and the flow path connected to the second reaction part 220 is 200. A hydrophilic line having a width of μm is provided, and a hydrophilic line having a width of 900 μm is provided at a flow path connected to the third reaction part 230, and a width of 2,000 μm is provided at a flow path connected to the fourth reaction part 240. The branch is provided with a hydrophilic line.

As a sample, a glucose solution at a concentration of 3 mM containing α-D-Glucose (Sigma-Aldrich) was prepared. In addition, a detection reagent was prepared by mixing a potassium iodide (Sigma-Aldrich) and a glucose oxidase (G2133-50KU, Sigma-Aldrich) extracted from a black mold in a weight ratio of 1: 1. 3 uL of the detection reagent was placed in the four fluid reaction parts and incubated at room temperature for 10 minutes, and then 70 uL of the glucose solution was supplied to the fluid receiving part.

When the glucose solution supplied to the fluid receiving part reaches the fluid reaction part, the glucose oxidase contained in the detection reagent supplied to the fluid reaction part reacts with the glucose contained in the glucose solution to react with hydrogen peroxide (H ₂ O ₂ ). Is generated. The generated hydrogen peroxide oxidizes iodine ions included in the detection reagent to iodine, and dark red color may be expressed in the reaction part by the iodine generated.

FIG. 11B is a diagram illustrating the detection of glucose on a microfluidic device according to an embodiment of the present invention, and FIGS. 11C to 11D illustrate the results of a glucose detection reaction in a microfluidic device according to an embodiment of the present invention. Drawing.

Specifically, FIG. 11B is an image of scanning the microfluidic device after 140 seconds and the microfluidic device after 480 seconds after supplying the glucose solution to the fluid receiving unit by using a scanner (TX129, Epson).

Referring to FIG. 11B, at the time when the glucose solution is supplied to the fluid receiving unit for 140 seconds, the color is expressed in the third fluid reaction unit 230 and the fourth fluid reaction unit 240. Afterwards, it can be seen that color is expressed in all four fluid reaction parts.

FIG. 11C shows the time when the glucose solution supplied to the fluid receiving unit reaches each fluid reaction unit, and is expressed in four fluid reaction units at a time of 140 seconds after the glucose solution is supplied to the fluid receiving unit. It is a figure which shows the average brightness of a color. The average brightness of the color expressed in the fluid reaction part was measured using the Image J program, the image scanned by the scanner.

Referring to FIG. 11C, the glucose solution first reaches the fourth fluid reaction part 240 connected to a flow path having a hydrophilic line having a width of 2,000 μm, and the fourth fluid reaction part 240 is the glucose solution. It can be seen that the average luminous intensity of the color expressed at the time of 140 seconds supplied to the fluid receiving portion is the largest. In addition, as the width of the hydrophilic line provided in the flow path increases, the time for the glucose solution to reach the fluid reaction part is reduced, and the color that is expressed at the time of 140 seconds after the glucose solution is supplied to the fluid receiving part. It can be seen that the average brightness increases.

FIG. 11D illustrates a result of measuring average luminosity of four fluid reaction units with time after the glucose solution is supplied to the fluid receiving unit. FIG. Referring to FIG. 11D, for 240 seconds after the glucose solution is supplied to the fluid receiving part, as the width of the hydrophilic line provided in the flow path increases, the average brightness of the color expressed in the fluid reaction part increases. In addition, when the glucose solution is supplied to the fluid receiving part and after 360 seconds, the third fluid reaction part 230 connected to the flow path having the hydrophilic line having the width of 900 μm is provided with the hydrophilic line having the width of 2,000 μm. It can be seen that the average brightness of the color that is expressed than the fourth fluid reaction part 240 connected to the flow path is larger.

Therefore, according to one embodiment of the present invention, by adjusting the width of the hydrophilic line formed in the flow path, it is possible to control the fluid movement speed in the flow path, the time the reaction proceeds in the fluid reaction unit by adjusting the fluid movement speed, The degree can be effectively controlled.

The foregoing description of the present invention is intended for illustration, and it will be understood by those skilled in the art that the present invention may be easily modified in other specific forms without changing the technical spirit or essential features of the present invention. will be. Therefore, it should be understood that the embodiments described above are exemplary in all respects and not restrictive. For example, each component described as a single type may be implemented in a distributed manner, and similarly, components described as distributed may be implemented in a combined form.

The scope of the present invention is shown by the following claims rather than the above description, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included in the scope of the present invention. do.

100, 110, 120: fluid receiving part
200, 210, 220, 230, 240: fluid reaction part
300, 310, 320, 330: Euro
400: fluid accelerator
410: hydrophilic line
500: fluid reduction unit
510: hydrophobic pattern
600: base film
700: micro euro film
800: cover film

Claims (14)

Fluid receiving portion; Fluid reaction unit; And at least one flow passage connected to at least one of the fluid receiving portion and the fluid reaction portion.
At least one of the flow path is a microfluidic device including a fluid accelerator including a hydrophilic line and a fluid decelerator including a plurality of hydrophobic island patterns spaced apart from each other,
The hydrophilic line is formed by printing or applying an ink containing at least one hydrophilic material selected from gold nanoparticles, platinum nanoparticles, silver nanoparticles, aluminum nanoparticles, and copper nanoparticles,
The hydrophobic island pattern prints or applies an ink including at least one hydrophobic material selected from polytetrafluoroethylene (PTFE), polystyrene, ethylene vinyl acetate copolymer, polycarbonate, polymethyl (meth) acrylate, and carbon nanotubes. Microfluidic device that is formed by. The method according to claim 1,
The microfluidic device includes two or more flow paths,
The flow path is a microfluidic device having a fluid movement speed different from each other. The method according to claim 1,
The fluid accelerator is microfluidic device to adjust the moving speed of the fluid by adjusting at least one of the length, the line width of the hydrophilic line. The method according to claim 1,
The ratio of the width of the flow path and the line width of the hydrophilic line is 1: 0.005 to 1: 1 microfluidic device. The method according to claim 1,
The fluid deceleration unit is a microfluidic device to adjust the moving speed of the fluid by adjusting at least one of the number of the hydrophobic island pattern, the separation distance, and the diameter of the island pattern. The method according to claim 1,
The ratio of the width of the flow path and the diameter of the hydrophobic island pattern is 1: 0.05 to 1: 0.2 microfluidic device. The method according to claim 1,
The fluid accelerator further comprises a plurality of hydrophobic island patterns. The method according to claim 1,
The fluid deceleration unit further comprises a hydrophilic line. The method according to claim 2,
The fluid receiving portion is all connected to the two or more flow paths, or a microfluidic device connected to each of the flow paths independently. The method according to claim 2,
The fluid reaction unit is all connected to the two or more flow paths, or a microfluidic device connected to each of the flow paths independently. delete delete The method according to claim 1,
A microfluidic device having a width of 50 μm or more and 10,000 μm or less. The method according to claim 1,
The microfluidic device comprises a cellulose substrate coated with a water repellent; Polymer substrates; Microfluidic device that is provided on a substrate selected from the group consisting of silicon-based glass.

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