KR102043161B1 - Microfluidic Device for Merging Micro-droplets and Method for Merging Micro-droplets Using Same - Google Patents

Abstract

The present invention relates to a microfluidic control device and a method for merging microdroplets using the same. According to the present invention, by using a simple and short microchannel structure, it is possible to efficiently control the flow of microdroplets and is possible to further simplify a microfluidic chip structure by treating merging of microdroplets and chemical reactions at once. Therefore, it is possible to apply on a lab-on-a-chip that can integrate a variety of pre-processing and analysis processes on a chip in a high speed and high efficiency. In addition, a particle size of a chemical reaction product can be controlled, thereby being able to be effectively applied in the field of fine chemistry that requires control of nano units.

Description

Microfluidic Device for Merging Micro-droplets and Method for Merging Micro-droplets Using Same}

The present invention relates to a microfluidic control device and a microfluidic control method for merging microfluidic droplets, and more particularly, to simplify the structure of the microchannels and control the particle size of the reaction product by simultaneously performing the merging and chemical reaction of the droplets. The present invention relates to a microfluidic control device and a method of merging microdroplets using the same.

Lab-on-a-chip technology means 'the lab is placed on a single chip', which uses materials such as plastic, glass, and silicon (silicon) to create micro- or nanoscale microchannels. This allows chips to be used to quickly replace experiments or research processes that can be performed in existing laboratories with only a small amount of samples or samples.

This chip can be used for diagnosing various cancers and measuring the number of cells in red blood cells and white blood cells with a single drop of fatigue, and can be applied to various fields such as agriculture, food, environment, and chemical industry as well as biological or pharmaceutical fields. Since the mid-2000s, technology has been rapidly developed and attracting attention.

A microfluidic chip is a biochip that analyzes the reaction of various biomolecules or sensors integrated in the chip while sending a small amount of analyte. Recently, the separation, synthesis and quantitative analysis of analytes Chips that can be used are being developed. This means using micromachining techniques to perform all steps on one chip, such as sample dilution, mixing, reaction, separation and quantification. In other words, pumps, valves, reactors, extractors, separation systems, etc., which are essential for the sample pretreatment process of an automated analysis device generally used for analysis of (bio) chemicals, can be implemented on one chip.

The microfluidic chip is characterized in that the final result can be obtained by sample injection only without going through various complicated steps. The use of such a biochip not only provides simplicity but also provides reliability for the result obtained by eliminating the experimental error of the tester as much as possible. It is expected to play a very important role in biotechnology.

Microfluidic chips are becoming more sophisticated due to advances in microelectromechanical systems (MEMS) and nanotechnology (nanotechnology or nanofabrication), which are devices ranging from tens of micrometers to tens of nanometers using materials other than silicon. It is possible to develop elaborately.

Droplet control technology is one of the most important techniques in this microfluidic art. Droplet control technology allows biochemical reactions and analysis to be carried out by moving droplets containing biological samples from one point to another without contamination and allowing them to quickly mix with the reactants. Can be classified and separated.

Because of this advantage, much research has been done on droplet control. For example, U.S. Patent Application Publication No. 2007/0195127 discloses that the first fluid droplets and the second fluid droplets have different sizes so that the moving speeds are different, and an electric field is applied to at least one of the two droplets, A technique for controlling a composite droplet is described, and Korean Patent Laid-Open Publication No. 10-2010-0060466 proposes a method of merging two fluid droplets by configuring a structure of a microfluidic channel in a 'c' shape.

This conventional droplet control technique complicates and grows the structure of the microfluidic chip by forming a complicated channel structure or controlling the size of the droplets and applying an electric field to control and merge the speeds of the two droplets. There was a problem. In addition, since the process of merging the fine droplets and the heating for the compound synthesis process must be performed separately, a special channel shape is required to perform each function, and the length of the channel becomes long. Complex channel geometries and long channel lengths present a risk of high pressure in the microchannels causing channel leakage or flow instability.

The inventors of the present invention have recognized and studied the above problems, and as a result, by controlling the flow of droplets by using an optical heating method, it is possible to form the microfluidic channel in a short and simple manner, and to control the particle size of the product. It has been found that the present invention has been completed.

It is an object of the present invention to provide a microfluidic control device having a simple structure and a short length of the fluid channel and capable of effectively controlling fluidic droplets in a simple manner.

Still another object of the present invention is to provide a method for efficiently controlling two or more types of microfluids using the microfluidic control device.

Another object of the present invention is to provide a method for controlling the particle diameter of a chemical reaction product using the microfluidic control device.

In order to achieve the above object, the present invention comprises a first fluid channel for injecting a first fluid into the flow channel in the continuous phase flow to form a first fluid droplet in the flow channel; A second fluid channel for injecting a second fluid into the flow channel through which the continuous phase flows to form second fluid droplets in the flow channel; A flow channel through which the first and second fluid droplets alternately flow; And a coalescing portion for locally heating the flow channel to stop the flow of the preceding fluidic droplets to induce coalescence of the first fluidic droplets and the second fluidic droplets.

In the present invention, the microfluidic control device includes a first fluid inlet for injecting a first fluid; And a second fluid inlet for injecting a second fluid.

In the present invention, the first fluid inlet and the second fluid inlet may each be connected to a feed pump.

In the present invention, the microfluidic control device includes a continuous phase injection channel for injecting the continuous phase into a flow channel; And a continuous phase inlet for injecting a continuous phase into the continuous phase injection channel.

In the present invention, the microfluidic control device may further include a second continuous phase injection channel for injecting a continuous phase into the flow channel to adjust the distance between the first fluid droplet and the second fluid droplet.

In the present invention, the local heating may be performed by laser beam irradiation.

In the present invention, the intensity of the laser beam is preferably 140 to 230mW.

In the present invention, the average diameter of the first fluid droplets and the second fluid droplets is preferably 70 to 150㎛.

In the present invention, the continuous phase is preferably a mixture of one or more selected from the group consisting of oleic acid, silicone oil, FC-40 oil, hexadecane and edible and ink. .

In the present invention, the first fluid and the second fluid are preferably an aqueous solution or an aqueous dispersion.

In the present invention, the reaction of the first fluid droplet and the second fluid droplet is preferably an endothermic reaction.

The invention also includes injecting a first fluid into a flow channel through which the continuous phase flows to form a first fluid droplet within the flow channel; Injecting a second fluid into the flow channel through which the continuous phase flows to form a second fluid droplet within the flow channel, wherein the first fluid droplet and the second fluid droplet alternately flow; And locally heating the flow channel to stop the flow of the preceding fluidic droplets to induce merging of the first and second fluidic droplets.

In the present invention, the method of merging the fine droplets may further comprise injecting a continuous phase into the flow channel to adjust the spacing of the first and second fluid droplets.

In the present invention, the local heating is preferably performed by laser beam irradiation.

The present invention also provides a method for controlling the particle diameter of a chemical reaction product using a microfluidic control device, comprising: injecting a first fluid into a flow channel through which a continuous phase flows to form first fluid droplets in the flow channel; Injecting a second fluid capable of reacting with the first fluid to produce nanoparticles into a flow channel in which the continuous phase flows to form a second fluid droplet within the flow channel, wherein the first fluid droplet and the second fluid droplet alternately. Allowing flow; And locally heating the flow channel to stop the flow of the preceding fluidic droplets to induce coalescence and chemical reaction of the first and second fluidic droplets, thereby controlling the intensity of the heating to control the first fluid. Provided is a method for controlling the particle size of a chemical reaction product using a microfluidic control device that controls the particle size of nanoparticles produced by chemical reaction of a droplet and a second fluid droplet.

According to the present invention, the flow of microdroplets can be efficiently produced using a simple and short microchannel structure, and the microfluidic chip structure can be further simplified because the microdroplet merge and chemical reaction can be processed at once. Accordingly, it is possible to apply to a lab-on-a-chip that can integrate a variety of pre-processing and analysis processes on the chip for high speed and high efficiency. In addition, it is possible to control the particle size of the chemical reaction product can be effectively applied in the field of fine chemistry requiring control of nano units.

1 is a structural diagram of a microfluidic control device according to an embodiment of the present invention.
2 is an enlarged view of some structures of a microfluidic control device according to an embodiment of the present invention.
Figure 3 is an enlarged view of the droplet merger of the microfluidic control device according to an embodiment of the present invention.
4 is an image taken by the CCD camera of the flow and merging of the micro droplets according to an embodiment of the present invention.
5 is an image of the product obtained in the discharge section according to an embodiment of the present invention.
6 is a graph showing the particle size of the nanoparticles according to the change in the intensity of the laser beam, according to an embodiment of the present invention.
7 is a graph showing the relationship between the intensity of the laser beam and the particle size of the nanoparticles according to an embodiment of the present invention.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In general, the nomenclature used herein is well known and commonly used in the art.

The present invention relates to a microfluidic control device in which coalescence and chemical reaction of microdroplets are unified, and the microfluidic control device of the present invention injects a first fluid into a flow channel through which a continuous fluid flows to inject a first fluid droplet into a flow channel. Forming a first fluid channel; A second fluid channel for injecting a second fluid into the flow channel through which the continuous phase flows to form second fluid droplets in the flow channel; A flow channel through which the first and second fluid droplets alternately flow; And a coalescing portion for locally heating the flow channel to stop the flow of the preceding fluidic droplets to induce merging of the first and second fluidic droplets.

According to the microfluidic control device of the present invention, since the merging of droplets and the chemical reaction are performed in one place, the structure of the microchannels can be simplified and formed in a short length, and the channel leakage problem or the flow instability problem can be solved. Can be.

Furthermore, the microfluidic device of the present invention can control the particle size of the product, making it suitable for application in the fine chemical field.

Hereinafter, the present invention will be described in more detail with reference to the accompanying drawings.

1 shows a microfluidic control device according to an embodiment of the present invention. The microfluidic control device of the present invention includes a flow channel 101 through which a continuous phase C flows, and a first fluid channel 211 and a second fluid channel 221 are connected to the flow channel 101, respectively. do.

The first fluid channel 211 injects the first fluid A injected from the first fluid inlet 212 into the flow channel 101 to generate the first fluid droplet a in the flow channel 101. . A continuous phase C is flowing in the flow channel 101, so that when the first fluid A is injected into the flow channel 101 through the first fluid channel 211, it can flow in the form of fine droplets. Make sure

Equally, the second fluid channel 221 injects the second fluid B injected from the second fluid inlet 222 into the flow channel 101 to inject the second fluid droplet b into the flow channel 101. Create in A continuous phase C is flowing in the flow channel 101, so that the second fluid B can flow in the form of fine droplets when injected into the flow channel 101 through the second fluid channel 221. Make sure

Supply pumps, such as a syringe pump (not shown), may be connected to the first and second fluid inlets 212 and 222 to inject fluid into the channel at a desired flow rate.

As shown in more detail in FIG. 2, the first fluid channel 211 and the second fluid channel 221 may be connected to the flow channel 101 at positions facing each other. A continuous phase C is flowing in the flow channel 101, and the first fluid A or the second fluid B is transferred through the first fluid channel 211 or the second fluid channel 221. Injection at an angle perpendicular to or close to the flow of phase C produces a first fluid droplet a or a second fluid droplet b flowing along the continuous phase C in the flow channel 101. . In addition, the first fluid droplet a and the second fluid droplet b injected from the first fluid channel 211 and the second fluid channel 221 are controlled to flow alternately in the flow channel 101. do.

The continuous phase C may be injected into the flow channel 101 from one or more continuous phase injection channels 311, and the continuous phase injection channel 311 may be injected from the continuous phase injection port 312. ) Is injected into the flow channel 101 to flow. The continuous phase inlet 312 may be connected to a supply pump such as a syringe pump to inject the continuous phase C into the channel at a desired flow rate.

In one embodiment of the invention, the continuous phase (C) allows fluid flowing in the first fluid channel 211 and the second fluid channel 221 to flow in the flow channel 101 in the form of droplets. It may also serve to adjust the distance between the first fluid droplet (a) and the second fluid droplet (b). In this case, the continuous phase C flows by the second continuous phase injection channel 321 connected to the flow channel 101 downstream of the first fluid channel 211 and the second fluid channel 221. May be injected into the channel 101.

The continuous phase (C) is preferably a liquid which is not mixed with the first fluid (A) and the second fluid (B). In addition, the continuous phase (C) may be composed of a material having a black or dark color, or may include a black or dark color pigment to more easily absorb the heat of the heat source described later.

The first fluid droplet a and the second fluid droplet b, which alternately flow in the flow channel 101 by the operation as described above, are separated from each other in the droplet merger 102 provided in the flow channel 101. Are merged. The droplet merger 102 is provided in a portion of the flow channel 101, in which the continuous phase C in the flow channel 101 is locally heated by a heat source. It is preferable to use a laser beam as the heat source.

3 is an enlarged view of the flow of droplets in the fluid merger 102. When the continuous phase (C) is locally heated by laser beam irradiation, the temperature of the continuous phase (C) rises, and when the fine droplets flowing in the heated portion approach, the fine liquid is caused by thermal capillary effects. Enemy flow will stop.

In the present invention, the "thermocapillary phenomenon" is a phenomenon in which the path of movement of the fine droplets is influenced by the droplet size (diameter), the temperature difference experienced by the droplets, and the viscosity of the continuous phase in the fine droplets passing through the microchannels. The thermocapillary phenomenon is classified into repulsive thermocapillary effects and attractive thermocapillary effects depending on whether the interfacial tension between the continuous phase and the dispersed phase increases or decreases with increasing temperature. do. The rebound thermocapillary phenomenon in which the interfacial tension between the continuous phase and the dispersed phase increases with increasing temperature shows that droplets of the dispersed phase tend to move in a lower temperature direction, and the interfacial tension decreases with increasing temperature. The droplets in the dispersed phase try to move in the direction of high temperature. In the present invention, by using the repulsive thermocapillary phenomenon in which the interfacial tension between the continuous phase and the dispersed phase increases with increasing temperature, the fine droplets act in the opposite direction to the portion where the flow is heated by the heat source, i.e., The phenomenon in which the repelling force acts in the direction opposite to the flow direction causes the fine droplets to temporarily stop.

When the continuous phase C absorbs heat provided from the heat source in the droplet merger 102 and heats it, the flow of the preceding droplet is stopped by the thermocapillary phenomenon, and thus, the trailing droplet and the preceding droplet collide with each other. Will merge with each other. Subsequently, when the volume of the fine droplets increases due to merging, the portion occupied by the continuous phase (C) is relatively reduced, and the area where the heat of the heat source is absorbed is reduced, thereby decreasing the temperature, and thus the action of the thermocapillary phenomenon is reduced. The reduced fine droplets begin to flow again.

In order to use the repulsive thermocapillary phenomenon in the present invention, the continuous phase and the dispersed phase should have a relationship in which the interfacial tension increases with increasing temperature. For example, oleic acid, which is frequently used due to biocompatibility, is used in a continuous phase, and as the dispersed phase, water which is most commonly used in microfluidics can be selected. In addition, since the properties of the interfacial tension with respect to the temperature is important, a variety of oils, such as silicone oil, FC-40 oil, hexadecane, edible oil may be used in the continuous phase.

From this point of view, in the present invention, the first fluid A and the second fluid B forming the fine droplets are preferably an aqueous solution or an aqueous dispersion. Thus, the microfluidic control device of the present invention can be most suitably applied to the chemical synthesis reaction of two or more components dissolved or dispersed in water.

In a preferred embodiment of the present invention, the microfluidic control device is used in the reaction for synthesizing cadmium sulfide (CdS) by the reaction of cadmium nitrate (Cd (NO ₃ ) ₂ ) and sodium sulfide (Na ₂ S) Can be. In this case, it is preferable that the first fluid A is an aqueous solution of cadmium nitrate, and the second fluid B is an aqueous solution of sodium sulfide, and the order of the first fluid and the second fluid is irrelevant.

Potassium thiocyanate (KSCN) and iron chloride (FeCl ₂ ) may also be used as reactions to synthesize iron cyanide (Fe (CN) ₆ ), which is used to simulate blood. Gold nanoparticles in addition to cadmium sulfide nanoparticles (Gold nanoparticles), gold nanorods (Cold nanorods), copper nanoparticles (Copper nanoparticles), cobalt nanoparticles (Cobalt nanoparticles) can be used to prepare a variety of nanoparticles.

In addition, in consideration of the application of droplet control, the dispersed phase is preferably an aqueous solution, and the continuous phase (C) is preferably an oil component. In the present invention, the continuous phase (C) used oleic acid (oleic acid) excellent in biocompatibility, but this is not limited, even if any other fluid that satisfies the condition of increasing the interfacial tension when the temperature is increased It's okay. For example, oil types such as silicone oil, FC-40 oil, hexadecane, and edible oil may be used.

In addition, in order to absorb energy from the heat source more efficiently, the continuous phase (C) is preferably a material having a black color or a dark color, or a black or dark color pigment. In a preferred embodiment of the present invention, a mixture of oleic acid and black ink is used as the continuous phase (C).

In the present invention, the droplet merger 102 may perform a chemical reaction by heating simultaneously with the merge of the droplets. Therefore, it is not necessary to provide a channel for merging droplets and a channel for heating and chemical reaction separately, so that the structure of the microfluidic chip can be simple and a short channel can be used, and the overall chip size can be made smaller. There is an advantage. From this point of view, the microfluidic control device of the present invention is most suitably applied to an endothermic reaction.

In addition, in the present invention, when the product produced by the reaction of the first fluid droplet (a) and the second fluid droplet (b) is a solid precipitate, it was confirmed that the crystal size of the solid precipitate can be controlled. In one embodiment of the invention, the nanoparticle size of the product increased in proportion to the intensity of the heat source applied. Specifically, as shown in Figure 7, as the intensity of the heat source is increased the diameter of the nanoparticles produced in the form of S-shaped curve. Such control of product particle size offers the possibility of being used for a variety of applications in the field of fine chemistry.

In a preferred embodiment of the present invention, the intensity of the heat source is preferably 140 to 230 mW. If the intensity of the heat source is less than 140 mW, the excess heat acting on the preceding droplet may result in a lack of the magnitude of the heat capillary force acting on the preceding droplet, which may cause the flow of the droplet to flow downstream without stopping the flow of the droplet. . In addition, when the intensity of the heat source is greater than 230mW, a problem may occur in which the droplets are mixed into the continuous fluid due to the influence of the surfactant in the ink solution and the high temperature of the fluid.

In the present invention, it is preferable that the first and second fluid droplets have a diameter of 50 to 300 mu m, and the diameter of 70 to 150 mu m is more preferable. If the diameter of the fluid droplets is too small, the thermal capillary phenomenon is difficult to act, and if too large, the droplets are crushed into a disc shape and severely interfere with the channel wall. In addition, since the laser beam, which is a heat source, passes through the transparent droplets, if the droplet size is too large, the energy of the heat source may not be sufficiently converted into heat.

The fine droplets a + b merged in the droplet merger 102 and undergoing the chemical reaction may be discharged through the discharge unit 102 provided at the end of the flow channel 102.

The microfluidic control device according to the present invention may be manufactured by a method generally used in the microfluidic chip art.

For example, the microfluidic control device of the present invention can be manufactured by the following method: preparing an upper substrate and a lower substrate; Forming microchannels and fluid inlets by using lithography (lithograpy) on the upper substrate; And bonding the upper substrate and the lower substrate.

The upper substrate is preferably made of a polymer, for example poly (dimethylsiloxane) (PDMS), and may be preformed in a molding manner before forming the channel by lithography. As the lower substrate, a silicon wafer substrate, a glass substrate, or the like can be used.

In the microfluidic control device according to an embodiment of the present invention, the width and height of the flow channel 101 are configured to have a width and a height at which the droplets can be stopped by a thermocapillary phenomenon while the liquid flows smoothly. It is preferable. For example, the width and height may range from 100 to 500 μm. In an embodiment of the present invention, a channel having a width of 400 μm and a height of 150 μm was manufactured and used.

In addition, the first and second fluid channels and the continuous phase injection channel may have a width and a height of 50 to 300 μm.

The microfluidic control device according to the present invention has the advantage of not requiring a complicated and long channel structure because it can control the merging of the microdroplets through simple channel structure and temperature control and simultaneously perform the merging and chemical reaction of the droplets. . In addition, since the size of the product particles can be controlled by controlling the intensity of the heat source, it has various applications in the field of fine chemistry.

Example

Hereinafter, the present invention will be described in more detail with reference to Examples. These examples are only to illustrate the invention, it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as limited by these examples.

Preparation Example: Preparation of Microfluidic Chips

In order to manufacture the microfluidic chip as shown in FIG. 1, channels and implants were formed using lithography on a PDMS substrate having a size of 5 cm x 2 cm and a thickness of 1.5 mm. The width and height of the flow channel were formed to be 400 μm and 150 μm, respectively, and the width and height of the first and second fluid channels and the continuous phase injection channel were formed to be 100 μm and 150 μm, respectively.

The formed PDMS substrate was attached to a slide glass to manufacture a microfluidic control device.

Experimental Example: Microfluidic Merging and Synthesis Experiment

The experiment was performed using the microfluidic chip prepared in the above preparation.

In the continuous phase, a solution containing 80% by weight of oleic acid and 20% by weight of black ink was used. As a first fluid, 0.3 mM Cd (NO ₃ ) ₂ .4H ₂ O (including 1% by weight of yellow food coloring) was prepared. 0.3 mM Na ₂ S.9H ₂ O was used as the 2 fluid. They were injected into the channel at a constant flow rate using a syringe pump (Pump 11 Plus, HARVARD APPARATUS). The first fluid and the second fluid were injected into the inlets of 212 and 222 of [FIG. 1] at a flow rate of 0.002 mL / hr, respectively, and the continuous fluid was 0.010 mL / hr of the inlets of 312 and 322 of [FIG. 1], respectively. And a flow rate of 0.100 mL / hr.

Experiments were performed by irradiating the droplet merger of the flow channel with 532 nm laser beams (LVI532CW2000FL-VA, Laserlab) at an intensity of 140, 165, 190, 215 and 240 mW, respectively. The intensity of the laser beam was measured using a power meter (1815C, Newport), and the full width at half maximum of the laser beam was 164 µm at 70 mW.

The flow of droplets was monitored and recorded in real time with LabView software (National Instrument) using a CCD camera (SDC-415A, Samsung). The results are shown in FIG. It can be seen that the fine droplets flowing alternately in FIG. 4 merge after being moved in the laser beam irradiation zone.

In addition, the product obtained in the outlet is shown in FIG. Cadmium sulfide (CdS) can be found in the black ink.

The size of the produced cadmium sulfide particles was analyzed according to the intensity of the laser beam through DLS (Dynamic Light Scattering) analysis, and the results are shown in FIG. 6.

Figure 6a is a particle distribution of the bulk synthesized cadmium sulfide without using the microfluidic chip of the present invention. It can be seen that the size of the nanoparticles is very nonuniform.

6b to 6f show particle distribution according to the intensity of the laser beam using the microfluidic chip of the present invention. The results are summarized in the table below.

division drawing Laser beam intensity (mW) Average particle size (nm) Example 1 6b 140 52.61 Example 2 Fig 6c 165 112.4 Example 3 6d 190 414.3 Example 4 6e 215 661 Example 5 6f 240 758

In the above table, it can be seen that as the intensity of the laser beam increases, the average particle size also increases.

The correlation between the laser beam intensity and the average particle size shown in the table is shown in FIG. 7. It can be seen that the average particle size graph according to the laser beam intensity represents the S-shape.

As described above in detail a specific part of the content of the present invention, for those of ordinary skill in the art, such a specific description is only a preferred embodiment, which is not limited by the scope of the present invention Will be obvious. Thus, the substantial scope of the present invention will be defined by the appended claims and their equivalents.

101 Flow Channel 102 Droplet Merging
103 outlet
211 first fluid channel 212 first fluid inlet
221 Second Fluid Channel 222 Second Fluid Inlet
311 continuous phase injection channel 312 continuous phase injection port
321 second continuous phase injection channel
A first fluid a first fluid droplet
B second fluid b second fluid droplet
C continuous phase

Claims (15)

A first fluid channel for injecting a first fluid into the flow channel through which the continuous phase flows to form a first fluid droplet within the flow channel;
A second fluid channel for injecting a second fluid into the flow channel through which the continuous phase flows to form second fluid droplets in the flow channel;
A flow channel through which the first and second fluid droplets alternately flow; And
Merging unit for locally heating the flow channel by irradiation of a laser beam of 140 to 230mW intensity to stop the flow of the preceding fluid droplets to induce the merging and reaction of the first and second fluid droplets
A microfluidic control device comprising:
And the local heating zone is larger than the diameter of the first and second fluid droplets. The method of claim 1,
A first fluid inlet for injecting a first fluid; And
Second fluid inlet for injecting a second fluid
Microfluidic control device comprising a. The method of claim 2,
And the first fluid inlet and the second fluid inlet are respectively connected to a feed pump. The method of claim 1,
A continuous phase injection channel for injecting the continuous phase into a flow channel; And
Continuous phase inlet for injecting continuous phase into the continuous phase injection channel
Microfluidic control device comprising a. The method of claim 1,
And a second continuous phase injection channel for injecting a continuous phase into the flow channel to adjust the spacing of the first and second fluid droplets. delete delete The method of claim 1,
Microfluidic control device, characterized in that the average diameter of the first fluid droplets and the second fluid droplets is 70 to 150㎛ The method of claim 1,
The continuous fluid is characterized in that the mixture of at least one selected from the group consisting of oleic acid (silic acid oil), silicone oil (silicon oil), FC-40 oil, hexadecane and edible and inks and ink, fine fluid control Device. The method of claim 1,
And the first fluid and the second fluid are aqueous solutions or water dispersions. The method of claim 1,
And the reaction of the first and second fluid droplets is an endothermic reaction. A method of merging microdroplets using a microfluidic control device,
Injecting a first fluid into the flow channel through which the continuous phase flows to form a first fluid droplet within the flow channel;
Injecting a second fluid into the flow channel through which the continuous phase flows to form a second fluid droplet within the flow channel, wherein the first fluid droplet and the second fluid droplet alternately flow; And
Locally heating the flow channel by laser beam irradiation at 140-230 mW to stop the flow of the preceding fluidic droplets to induce coalescence and reaction of the first and second fluidic droplets;
As a method of merging fine droplets comprising:
And wherein said local heating zone is larger than the diameter of said first and second fluid droplets. The method of claim 12,
And injecting a continuous phase into the flow channel to adjust the spacing of the first fluid droplet and the second fluid droplet. delete A method of controlling the particle diameter of a chemical reaction product using a microfluidic control device,
Injecting a first fluid into the flow channel through which the continuous phase flows to form a first fluid droplet within the flow channel;
Injecting a second fluid capable of reacting with the first fluid to produce nanoparticles into a flow channel in which the continuous phase flows to form a second fluid droplet in the flow channel, wherein the first fluid droplet and the second fluid droplet alternately. Allowing flow; And
Locally heating the flow channel by laser beam irradiation of 140-230 mW intensity to stop the flow of the preceding fluidic droplets to induce coalescence and chemical reaction of the first and second fluidic droplets;
Including,
Controlling the intensity of the heating to control the particle size of the nanoparticles produced by the chemical reaction of the first and second fluid droplets,
And wherein said local heating zone is larger than the diameters of said first and second fluid droplets.

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