KR102035649B1 - A composition for rice bacterial blight suppressing activity - Google Patents
A composition for rice bacterial blight suppressing activity Download PDFInfo
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Abstract
본 발명은 스트렙토마이세스 MJM8519 균주로부터 생산되는 Antibiotic FD893을 포함하는 벼흰잎마름병 방제용 조성물에 관한 것이므로, 본 발명에 따른 벼흰잎마름병 방제용 조성물은 세계 3대 식물 중 하나인 벼의 가장 큰 피해를 야기하는 벼흰잎마름병을 억제하여 벼 재배 지역의 피해를 최소화할 수 있다.The present invention is Streptomyces because it relates to a composition for paddy huinip blight control containing Antibiotic FD893 produced from MJM8519 strain, for rice huinip blight controlling composition according to the invention are the most affected, one of the three major plant rice By suppressing the rice leaf blight that causes, it is possible to minimize the damage of the rice cultivation area.
Description
본 발명은 스트렙토마이세스 MJM8519(Streptomyces MJM8519) 균주로부터 생산되는 Antibiotic FD893을 포함하는 벼흰잎마름병 방제용 조성물에 관한 것이다. The present invention relates to a composition for the control of rice blight huinip containing Antibiotic FD893 produced from Streptomyces MJM8519 (Streptomyces MJM8519) strain.
벼흰잎마름병의 병징은 묘판 후기에 아래 잎의 가장자리에 물에 젖은 듯한 작은 병반이 나타나며, 그 표면에 분출된 세균의 마른 딱지가 형성된다. 발병된 지 며칠이 지나면 병반이 노란색으로 변하게 되고, 잎의 끝부분부터 하얗게 마르면서 잎이 죽게 된다. 감수성이 매우 높은 품종에서는 묘를 이앙한 후부터 분얼 최성기까지의 벼가 갑자기 말라죽는 급성형 병징(kresek)이 있는데 잎에서는 뚜렷한 병징이 없고, 잎의 가장자리나 잎 끝에 좁쌀보다 작은 세균의 마른 딱지가 보일 뿐이다. 분얼 최성기 이후 성숙된 잎에 나타난 병증상은 5∼6cm 아래의 잎 가장자리에 물에 젖은 듯한 작은 무늬가 생긴다. 아침 이슬이 맺힐 때 이 병반의 표면에는 옅은 우유빛의 이슬이 맺히며, 이것이 말라서 황색의 딱지로 남아 있는 것을 볼 수 있다. 7∼13일간 병이 진전되면 병반이 커지면서 황색∼등황색이 되고, 건강한 잎조직과의 경계는 물에 젖은 듯한 수침상으로 된다. 병반은 잎의 한쪽 또는 양쪽 가장자리에 형성되어 진전되며, 후에 잎 중앙맥까지 번지며 물결 모양으로 확대된다. 병이 더 진전되면 황백색 병반이 백색∼회백색으로 변하고, 부생균에 의해 오염되면서 더러운 색깔로 변한다.The symptoms of rice leaf blight are late stages of seedlings, with small lesions on the edges of the lower leaves, as if soaked with water. After several days of onset, the lesions turn yellow, and the leaves die off as they dry out from the tip of the leaves. In highly susceptible varieties, there is an acute type of kresek, in which the rice suddenly dies after transplanting the seedlings to the peak of the grains. It is only. Symptoms of mature leaves after the peaking of the vegetation have small patterns that seem to be wet with water at the edge of the leaves 5-6 cm below. When morning dew forms, the surface of this lesion forms a light milky dew, which can be seen to dry out and remain a yellow scab. When the disease progresses for 7 to 13 days, the lesion grows and becomes yellow to orange, and the boundary with healthy leaf tissue becomes water-soaked needles. Lesions are formed on one or both edges of the leaf and progress, later spreading to the leaf central veins and expanding in a wave shape. As the disease progresses further, the yellowish white lesions turn white to grayish and become dirty colors as they are contaminated by by-products.
벼흰잎마름병원균인 Xanthomonas oryzae pv . Oryzae는 그람음성의 호기성 세균으로서 단극모를 가지고 있고, 막대모양이다. 고체배지 상에서 황색의 집락(colony)을 형성하며, 자라는 속도는 다소 느리고, 생육 최적온도는 26∼30℃이다. 병반에 말라붙은 세균덩어리나 병들어 죽은 식물체 속에 남아 있는 병원세균은 8개월 이상 살 수 있으나, 고온 ·다습한 환경에서는 짧은 시일 내에 죽는다. Xanthomonas , a rice blight pathogen oryzae pv . Oryzae may have unipolar know as the gram-negative aerobic bacteria, it is rod-shaped. Yellow colonies are formed on the solid medium, the growth rate is rather slow, and the optimum growth temperature is 26-30 ° C. Bacteria that have dried on diseased pathogens and pathogens that remain in diseased and dead plants can live for more than eight months, but die in a short period of time in hot and humid environments.
발병을 일으키는 생태는 병원세균은 수로나 저수지에 흔히 볼 수 있는 잡초 중에 겨풀, 나도겨풀의 근권이나 병든 볏짚에서 월동한다. 월동한 병원세균들이 1차전염원이 되어 논물에 유입된 후 수면에 접촉된 벼잎의 배수선이나 기공으로 침입하여 병반을 형성하게 된다. 병반에서 증식된 병원세균이 2차 전염원이 되어 논물 속으로 배출되거나 직접 병반과 건전한 벼 조직의 접촉에 의하여 전염된다. 강한 바람은 벼에 상처를 주어 병원세균이 침입하기 쉽게 하고, 병반부에서 누출된 병원세균을 바람에 실어 50m 이상 운반한다. 또한 태풍에 동반된 많은 비로 인해 벼가 물에 잠기게 되면 배수선이나 기공을 통한 병원세균의 침입이 더욱 쉬워진다. 7월 평균기온이 22∼24℃로 저온일 때, 7월의 강수량이 200mm 이상으로 많은 비가 올 때, 15m/sec 이상의 강풍이 불 때 이 병의 발생이 특히 많아진다. 모판이나 본답에서 질소비료를 많이 주게 되면 벼가 감수성으로 되어 이 병의 발병을 촉진시킨다 The pathogenic ecology causes the pathogens to overwinter in the rhizome, rhizome, or diseased rice straw, among the weeds commonly found in waterways and reservoirs. Overwintering pathogens become the primary source of infection and enter the paddy fields and then invade the drainage lines or pores of rice leaves in contact with the water to form lesions. The pathogens propagated in the lesions become secondary infectious agents and are discharged into the paddy field or transmitted by direct contact between the lesions and healthy rice tissue. Strong winds damage rice, making it easier for invading pathogens, and carrying pathogens leaked from lesions in the wind and transporting more than 50m. In addition, when the rice is submerged due to the heavy rain accompanied by the typhoon, the invasion of pathogens through drainage lines or pores becomes easier. When the average temperature in July is low at 22 to 24 ° C, when the rainfall in July is more than 200 mm, when the rain is heavy, when the strong wind blows more than 15 m / sec, the disease is particularly high. If a large amount of nitrogen fertilizer is given in the bed sheet or the answer, the rice becomes susceptible to the development of the disease.
그리고 스트렙토마이세스와 같은 방선균으로부터 이미 다양한 항생물질이 분리되었으나, 현재 상기 항생물질이 정확히 어떠한 미생물에 대하여 항생효과를 나타내는지에 대해서 연구가 진행중에 있다.Although various antibiotics have already been isolated from actinomycetes such as Streptomyces, research is currently being conducted on exactly which microorganisms have antibiotic effects.
본 발명자들은 방선균으로부터 분리한 항생물질인 Antibiotic FD893가 그람 음성 균주인 벼흰잎마름병의 원인 균주에 대한 억제 효과를 확인함으로써 본 발명을 완성하였다.The present inventors completed the present invention by confirming the antibiotic FD893 antibiotic isolated from actinomycetes against the causative strain of Gram-negative rice leaf blight.
본 발명은 벼흰잎마름병의 활성을 억제하는 벼흰잎마름병 방제용 조성물을 제공하고자 한다.The present invention is to provide a composition for the control of rice white leaf blight inhibiting the activity of rice white leaf blight.
본 발명은 Antibiotic FD893을 포함하는 벼흰잎마름병 방제용 조성물을 제공한다.The present invention provides a composition for controlling rice bran leaf blight comprising Antibiotic FD893.
또한 본 발명은 (a) 수탁번호 KACC92147P로 기탁된 스트렙토마이세스 MJM8519 균주를 배양하는 단계; (b)상기 배양물을 유기용매로 추출하는 단계; (c)상기 추출물에 대해 크로마토그래피를 수행하여 활성분획을 수득하는 단계; (d)상기 활성분획을 정제하는 단계를 포함하는 Antibiotic FD893의 제조방법을 제공한다.In another aspect, the present invention (A) culturing the Streptomyces MJM8519 strain deposited with accession number KACC92147P; (b) extracting the culture with an organic solvent; (c) performing chromatography on the extract to obtain an active fraction; (d) It provides a method for producing Antibiotic FD893 comprising the step of purifying the active fraction.
또한 본 발명은 상기 방제용 조성물을 벼, 벼의 종자 또는 벼의 재배 토양에 처리하는 것을 특징으로 하는 벼흰잎마름병 방제 방법을 제공한다.In another aspect, the present invention provides a method for controlling rice white leaf blight, characterized in that the treatment composition for the treatment of rice, rice seeds or rice cultivation soil.
본 발명에 따른 벼흰잎마름병 방제용 조성물은 세계 3대 식물 중 하나인 벼의 가장 큰 피해를 야기하는 벼흰잎마름병을 억제하여 벼 재배 지역의 피해를 최소화할 수 있다.The composition for controlling rice leaf blight according to the present invention can minimize the damage of rice cultivation area by suppressing rice leaf blight causing the biggest damage of rice, which is one of the three largest plants in the world.
도 1은 스트렙토마이세스 MJM8519 균주에서 유래된 Antibiotic FD893의 화학식이다.
도 2는 스트렙토마이세스 MJM8519 균주에서 유래된 Antibiotic FD893을 RP-HPLC 분리 후, UPLC-Q-TOF/MS 및 UHPLC-LTQ-IT-MS/MS로 분석한 결과를 나타낸 크로마토그램 피크 패턴이다.
도 3은 스트렙토마이세스 MJM8519 균주에서 유래된 Antibiotic FD893 및 DAPG(2,4-diacetylphloroglucinol)의 벼잎생엽절편 스크리닝 결과를 나타낸 그래프이다; 1컬럼: 음성대조군, 2-11컬럼: HPLC 분획, 12컬럼: 양성대조군(DAPG, 2,4-Diacetylphloroglucinol). row 1 내지 8은 HPLC 분획을 각각 희석한 것(row 1 = 1/2, row 2 = 1/4, row 3 = 1/8, row 4 = 1/16, row 5 = 1/32, row 6 = 1/64, row 7 = 1/128, row 8 = 1/256).1 is a formula of Antibiotic FD893 derived from Streptomyces strain MJM8519.
Figure 2 is a chromatogram peak pattern showing the results of analysis by UPLC-Q-TOF / MS and UHPLC-LTQ-IT-MS / MS after RP-HPLC separation of Antibiotic FD893 derived from Streptomyces MJM8519 strain.
3 is Streptomyces It is a graph showing the screening results of leaf leaf sections of Antibiotic FD893 and DAPG (2,4-diacetylphloroglucinol) derived from MJM8519 strain; Column 1: negative control, 2-11 columns: HPLC fractions, column 12: positive control (DAPG, 2,4-Diacetylphloroglucinol).
본 발명은 화학식Ⅰ의 Antibiotic FD893을 포함하는 벼흰잎마름병 방제용 조성물을 제공한다.The present invention provides a composition for controlling rice bran leaf blight comprising Antibiotic FD893 of formula (I).
본 발명은 상기 Antibiotic FD893이 수탁번호 KACC92147P로 기탁된 스트렙토마이세스 MJM8519(streptomyces MJM8519) 균주에서 유래된 것을 특징으로하는 벼흰잎마름병 방제용 조성물을 제공한다.The present invention is Streptomyces MJM8519 ( streptomyces deposited with the antibiotic FD893 accession number KACC92147P MJM8519) provides a composition for the control of rice blight blight, characterized in that derived from the strain.
또한 본 발명은 상기 벼흰잎마름병이 잔토모나스 속 세균에 의해 발생되는 식물병임을 특징으로하는 벼흰잎마름병 방제용 조성물을 제공한다.In another aspect, the present invention provides a composition for controlling rice white leaf blight disease, characterized in that the rice plant blight is a plant disease caused by bacteria of the genus Xanthomonas .
본 발명에 따른 한 구체예에서, 스트렙토마이세스 MJM8519 유래 Antibiotic FD893이 벼흰잎마름병을 유발하는 병원균 Xanthomonas oryzae pv . Oryzae에 대한 우수한 항균활성을 가진다는 것이 본 발명자들에 의하여 확인되었다.In one embodiment according to the invention, Streptomyces MJM8519-derived Antibiotic FD893 is a pathogen that causes rice leaf blight Xanthomonas oryzae pv . It was confirmed by the present inventors to have excellent antimicrobial activity against Oryzae .
특히, 식물병원체에 대한 억제 활성이 알려진 자연 페놀 화합물인 Pseudomonas fluorescens 그람음성박테리움 DAPG(2,4-diacetylphloroglucinol)와 유사한 벼희잎마름병 병원균에 대한 저항활성을 보임을 실험적으로 밝혔다(실시예 3)In particular, it was experimentally shown to exhibit resistance to rice rot leaf blight pathogens similar to Pseudomonas fluorescens Gram-negative bacterium DAPG (2,4-diacetylphloroglucinol), a known natural phenolic compound that inhibits plant pathogens (Example 3).
본 발명은 (a) 수탁번호 KACC92147P로 기탁된 스트렙토마이세스 MJM8519(Streptomyces MJM8519) 균주를 배양하는 단계; (b)상기 배양물을 유기용매로 추출하는 단계; (c)상기 추출물에 대해 크로마토그래피를 수행하여 활성분획을 수득하는 단계; (d)상기 활성분획을 정제하는 단계를 포함하는 Antibiotic FD893의 제조방법을 제공한다.The present invention comprises the steps of culturing the Streptomyces MJM8519 (Streptomyces MJM8519) strain deposited as (a) accession No. KACC92147P; (b) extracting the culture with an organic solvent; (c) performing chromatography on the extract to obtain an active fraction; (d) It provides a method for producing Antibiotic FD893 comprising the step of purifying the active fraction.
상기 활성분획을 수득하는 단계 후에, (e) 상기 수집된 물질에 대해 질량분석을 수행하여 Antibiotic FD893을 확인 하는 단계를 더 포함할 수 있다.After the step of obtaining the active fraction, (e) performing a mass spectrometry on the collected material may further comprise the step of identifying the Antibiotic FD893.
상기 유기용매는 메탄올, 에탄올, 부탄올, 주정, 아세톤, 아세트산 에틸, 에테르, 클로로포름, 펜탄, 헥산, 헵탄, 노난, 데칸, 벤젠, 톨루엔 및 자이렌으로 이루어진 군에서 선택되는 어느 하나 이상인 것일 수 있으나, 이에 한정되지 않는다. 여기서 '주정'이란 전분질 원료 또는 당질 원료를 발효시킨 후 증류하여 만든 에탄올을 의미한다. 상기 유기용매로써는 아세트산 에틸, 메탄올, 에탄올, 부탄올 및 주정 등 알코올인 것이 바람직하고, 아세트산 에틸인 것이 가장 바람직하나, 이에 한정되지 않는다. The organic solvent may be any one or more selected from the group consisting of methanol, ethanol, butanol, spirits, acetone, ethyl acetate, ether, chloroform, pentane, hexane, heptane, nonane, decane, benzene, toluene and xylene, It is not limited to this. Here, 'alcohol' refers to ethanol made by distilling fermented starch raw material or sugar raw material. The organic solvent is preferably ethyl acetate, methanol, ethanol, butanol and alcohol such as alcohol, and most preferably ethyl acetate, but not always limited thereto.
상기 크로마토그래피는 이에 한정되는 것은 아니나, 흡착제로서 실리카겔, 역상실리카겔, 세파덱스류, 다이아이온 레진류, 알루미나, 활성탄, 규산마그네슘, 탄산칼슘, 인산칼슘, 인산마그네슘, 마그네시아 또는 수산화칼륨 등을 사용할 수 있으며, 세파덱스류를 사용할 수 있다. 보다 구체적으로는, 세파덱스 컬럼 크로마토그래피를 수행한 후 고성능 액체 크로마토그래피를 한번 더 수행할 수 있다.The chromatography is not limited thereto, but silica gel, reversed phase silica gel, Sephadex, diion resin, alumina, activated carbon, magnesium silicate, calcium carbonate, calcium phosphate, magnesium phosphate, magnesia or potassium hydroxide may be used as the adsorbent. Sephadex can be used. More specifically, after performing Sephadex column chromatography, high performance liquid chromatography may be performed once more.
본 발명에 따른 Antibiotic FD893을 생산하는 스트렙토마이세스 MJM8519균주 또는 그 배양 여액을 포함하는 벼흰잎마름병 방제용 조성물을 제공한다. The present invention provides a composition for controlling rice leaf blight comprising Streptomyces MJM8519 strain producing the antibiotic FD893 or a culture filtrate thereof.
상기 Antibiotic FD893을 유효성분으로 포함하여 벼흰잎마름병 방제용 살균제를 제조하기 위하여 본 발명이 속하는 기술분야에서 통상적으로 사용되는 충전제, 중량제, 계면활성제 등을 혼합하여 벼흰잎마름병 방제용 조성물을 제조하는 것이 가능하다.Including the antibiotic FD893 as an active ingredient to prepare a fungicide for the control of rice blight blight to prepare a composition for the control of rice blight blight by mixing fillers, weighting agents, surfactants, etc. commonly used in the art to which the present invention belongs. It is possible.
본 발명에 따른 방제용 조성물을 벼, 벼의 종자 또는 벼의 재배 토양에 처리하는 것을 특징으로 하는 벼흰잎마름병 방제 방법을 제공한다.Provided is a method for controlling rice white leaf blight, characterized in that the control composition according to the present invention is treated with rice, rice seeds or cultivated soil of rice.
이하 본 발명에 따르는 실시예 및 본 발명에 따르지 않는 비교예를 통하여 본 발명을 보다 상세히 설명하나, 본 발명의 범위가 하기 제시된 실시예에 의해 제한되는 것은 아니다.Hereinafter, the present invention will be described in more detail with reference to examples according to the present invention and comparative examples not according to the present invention, but the scope of the present invention is not limited to the following examples.
<< 실시예Example 1> 1> 벼흰잎마름병에On rice white leaf blight 항균 활성을 갖는 Having antimicrobial activity 스트렙토마이세스Streptomyces MJM8519MJM8519 균주의 분리 및 동정 Isolation and Identification of Strains
스트렙토마이세스(Streptomyces sp .)를 분리하기 위하여, 함박산(경기, 한국)에서 얻은 1g의 토양 샘플을 세척하여 60℃에서 30분 동안 가열하고, 스트렙토마이세스를 분리하기 위해 가열된 샘플을 HV(Humic acid vitamin) 한천 배지로 옮겼다. Streptomyces (Streptomyces sp . 1g soil samples from Hambaksan (Gyeonggi, Korea) were washed and heated at 60 ° C for 30 minutes, and the heated samples were separated with HV (Humic acid vitamin) agar medium to isolate Streptomyces . Moved.
한천에서 자란 단일 콜로니를 분리하여 GSS 배지(가용 녹말 1%, 글루코스 2%, 대두박 2.5%, 쇠고기 추출물 0.1%, 효모 추출물 0.4%, NaCl 0.2%, K2HPO4 0.025%, CaCO3 0.2%, pH 7.2)에서 200rpm, 28℃에서 7일 간 배양하고 16개의 2L 삼각 플라스크에 650 ml씩 나누어 담았다.Single colonies grown in agar were isolated and GSS medium (
스트렙토마이세스를 동정하기 위하여, 전장 16S rDNA를 유니버셜 프라이머 세트(universal primer set)인 27F 프라이머(5'-AGA GTT TGA TCA TGG CTC AG-3')(서열번호 1) 및 1492R 프라이머(5'-GGA TAC CTT GTT ACG ACT T-3')(서열번호 2)를 이용하여 증폭하였고, 상기 서열을 NCBI 홈페이지(National Center for Biotechnology Information; http://www.ncbi.nlm.nih.gov/)에서 분석하였다.To identify Streptomyces , full-length 16S rDNA was run on a universal primer set of 27F primer (5'-AGA GTT TGA TCA TGG CTC AG-3 ') (SEQ ID NO: 1) and 1492R primer (5'-). Amplified using GGA TAC CTT GTT ACG ACT T-3 '(SEQ ID NO: 2), and the sequence was obtained from the NCBI homepage (National Center for Biotechnology Information; http://www.ncbi.nlm.nih.gov/). Analyzed.
그 결과 분리된 균주는 스트렙토마이세스 속 균주와 99%의 상동성을 가짐을 확인하였다. 따라서 분리된 스트렙토마이세스 균주를 스트렙토마이세스 MJM8519로 명명하였으며, 2016년 10월 13일자로 KACC에 기탁하여 수탁번호 KACC92147P를 부여 받았다.As a result, it was confirmed that the isolated strain had 99% homology with the strain of Streptomyces genus. Therefore, the isolated Streptomyces strain was named Streptomyces MJM8519, and was deposited with KACC on October 13, 2016 to receive accession number KACC92147P.
<< 실시예Example 2> 2> 스트렙토마이세스Streptomyces MJM8519MJM8519 균주로부터 From strain 벼흰잎마름병에On rice white leaf blight 항균 활성을 갖는 Antibiotic FD893의 분리 및 동정 Isolation and Identification of Antibiotic FD893 with Antimicrobial Activity
벼흰잎마름병 억제 활성 화합물을 분리하기 위하여, 스트렙토마이세스 MJM8519 균주를 실시예 1에 따른 GSS 배지에서 28일 간 배양한 후 배양액을 제거하고 균체에 80% 메탄올을 첨가하여 공지의 방법으로 마쇄하였다. 상기 마쇄한 균개에 에틸아세테이트 300ml를 첨가하고 4℃에서 10분간 15,000rpm으로 원심분리 한 후, SepPak SPE(고체상 추출, Solid phase extraction) 시료를 이용한 세파덱스(Sephadex) LH-20 컬럼 크로마토그래피로 분획하였다. 이후, C18 역상 고성능 액체 크로마토그래피(reverse-phase high-performance liquid chromatography, C18 RP-HPLC)로 활성 화합물을 분리하였으며, HPLC의 20번째 분획에서 활성 화합물의 함량이 가장 높은 것을 확인하였다.In order to isolate the rice leaf blight inhibitory active compound, the Streptomyces MJM8519 strain was incubated for 28 days in GSS medium according to Example 1, the culture medium was removed, and the cells were ground by a known method by adding 80% methanol. 300 ml of ethyl acetate was added to the ground fungus, and centrifuged at 15,000 rpm for 10 minutes at 4 ° C., and then fractionated by Sephadex LH-20 column chromatography using SepPak SPE (Solid phase extraction) sample. It was. Since, C 18 reverse phase high performance liquid chromatography (reverse-phase high-performance liquid chromatography, C 18 RP-HPLC) was separated into the active compound, it was confirmed that from the 20 th fraction of the HPLC with the highest content of the active compound.
수득한 활성 화합물을 UPLC-Q-TOF/MS(ultra-performance liquid chromatography quadrupole time of flight mass spectrometry)로 분석한 결과, 분자량은 706.47 m/z, 분자식은 C40H66O10 이었다(도 2). The obtained active compound was analyzed by UPLC-Q-TOF / MS (ultra-performance liquid chromatography quadrupole time of flight mass spectrometry), and the molecular weight was 706.47 m / z and the molecular formula was C 40 H 66 O 10 (FIG. 2). .
<< 실시예Example 3> 3> 스트렙토마이세스Streptomyces MJM8519MJM8519 균주 유래 Antibiotic FD893의 Strain-derived Antibiotic FD893 벼흰잎마름병에On rice white leaf blight 대한 억제 활성 Inhibitory activity
Antibiotic FD893의 벼흰잎마름병에 대한 억제 활성을 확인하기 위하여, 96웰 플레이트에서 2배 연속 희석법을 수행하였다. 벼흰잎마름병의 병원균인 Xanthomonas oryzae pv . Oryzae KACC 10331의 배양 배지를 벼잎생엽절편에 투여하였다. 벼잎생엽절편의 병원균 감염 정도는 육안으로 확인하였다.In order to confirm the inhibitory activity of Antibiotic FD893 against rice leaf blight, two-fold serial dilutions were performed in 96-well plates. Xanthomonas oryzae , a pathogen of rice blight pv . Culture medium of Oryzae KACC 10331 was administered to the leaf leaf sections. The extent of pathogen infection of rice leaf slices was visually confirmed.
그 결과, Antibiotic FD893에 해당하는 HPLC 분획을 투여한 96웰 플레이트의 9컬럼 내 대부분의 벼잎생엽절편이 황색이 아닌 녹색을 나타내어, 양성대조군인 DAPG(2,4-Diacetylphloroglucinol)과 같은 병원균 감염 억제 효과가 있음을 확인하였다(도 3).As a result, most of the leaf leaf fragments in 9 columns of 96-well plate treated with HPLC fractions corresponding to Antibiotic FD893 showed green instead of yellow, and inhibited pathogen infection such as positive control DAPG (2,4-Diacetylphloroglucinol). It was confirmed that there is (Fig. 3).
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Claims (6)
[화학식Ⅰ]
[Formula I]
상기 Antibiotic FD893은 수탁번호 KACC92147P로 기탁된 스트렙토마이세스 MJM8519 (Streptomyces MJM8519) 균주에서 유래된 것인 벼흰잎마름병 방제용 조성물.The method of claim 1,
The Antibiotic FD893 is a Streptomyces deposited as accession No. KACC92147P MJM8519 (Streptomyces MJM8519) A composition for controlling rice white leaf blight which is derived from a strain.
상기 벼흰잎마름병은 잔토모나스(Xanthomonas) 속 세균에 의해 발생되는 식물병임을 특징으로하는 벼흰잎마름병 방제용 조성물.The method of claim 1,
The rice white leaf blight is a composition for controlling rice white leaf blight, characterized in that the plant disease caused by bacteria of the genus Xanthomonas ( Xanthomonas ).
A method of controlling rice white leaf blight, comprising treating the composition for control of any one of claims 1 to 3 with rice, seed of rice or cultivated soil of rice.
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