KR102034065B1 - Mesoporous Carbon-Silver Composite With Antibacterial Activity And Menufacturing Method Thereof - Google Patents

Abstract

The present invention provides a method for producing a mesoporous carbon-silver nanocomposite using sugar as a carbon raw material and a small amount of silver nitrate, and a mesoporous carbon-silver composite produced thereby.
The method for producing the mesoporous carbon-silver complex of the present invention, the mesoporous carbon-silver complex produced by the same, and the water treatment method using the same for the sterilization and purification of water infected by water-borne pathogens in the field of antimicrobial agents, water treatment agents and water treatment methods. It can be usefully used as an effective water treatment agent.

Description

Mesoporous Carbon-Silver Composite With Antibacterial Activity And Menufacturing Method Thereof}

The present invention relates to a mesoporous carbon-silver complex having an antibacterial activity and a method for preparing the same, and more particularly, to a mesoporous carbon-silver complex having a strong antibacterial activity against gram-positive and gram negative bacteria and a sugar. It relates to a production method thereof.

Mesoporous (porous) materials have a large surface area and a large number of pores, making them widely used in the field of catalysis, adsorption and water / air purification. They have been used as antibacterial filters for leaching microorganisms due to the adsorption of microorganisms to a large surface area and the adsorbed microorganisms are unable to pass through small mesopores. Regarding the antimicrobial properties of mesoporous materials, there have previously been several reports of the antibacterial effects of mesoporous carbon-lysozyme complexes, and mesoporous silica nanotubes comprising titanium oxide.

Silver (Ag) is well known as a promising antibacterial agent, and is sometimes produced and used as a filter using the antibacterial effect of silver. In particular, silver nanoparticles (AgNP) having a size of 1 to 100 nm are attracting attention because their contact with microorganisms is improved by their large surface area. Silver (Ag) is a soft acid, which reacts with a soft base containing S- or P- in bacterial cell membranes or DNA, resulting in damage to the membrane consisting of proteins and lipids. On the other hand, AgNPs tend to associate with each other, so that the size of the particles increases due to aggregation between AgNPs and the surface area decreases the antimicrobial effect of silver. Therefore, AgNPs need to be dispersed in water while preventing AgNP aggregation. The use of large amounts of silver could potentially be harmful to the environment. In addition, there has been a continuous demand for antimicrobial agents that have antimicrobial activity and durability while being environmentally friendly and recyclable.

Conventional patents on antimicrobial agents using silver include a glass water treatment agent (Japanese Patent Laid-Open No. 62-210098) made of soluble glass containing monovalent silver ions or a zirconium phosphate compound carrying and bonding silver; There was a water purification agent (Japanese Patent Laid-Open No. 7-222983) having excellent antimicrobial properties made of activated carbon.

However, for pure water such as beverage use and circulating water, although it is optimal to elute only a small amount of silver ions that are antibacterial components, and other substances are not eluted or dissolved, Patent Document 1 discloses other than silver which is an active ingredient. In addition to the dissolution of the glass component, there was a problem that control of the silver concentration in the aqueous solution was difficult. In addition, Patent Document 2 is not easy to control the persistence over a long period of time and a constant silver concentration in water, and was not suitable for water treatment.

Korean Patent Publication No. 10-2009-0080161 (2009.07.24.)

Applied Material & Interfaces 2017, 9 (23), pp 19371-19379 (October 28, 2016)

Therefore, the present inventors are studying the synergistic effect of mesoporous material and silver nanoparticles to develop an environmentally friendly water treatment agent, and the matrix having a uniform size of mesopores is used to filter harmful microorganisms through filtration and adsorption according to a large surface area. In light of its usefulness in the removal, it has been developed a method for preparing a structurally aligned MS or MC and Ag complex, and found that the prepared mesoporous material and silver complex exhibit excellent antibacterial effects against pathogenic bacteria. It was.

Accordingly, an object of the present invention is to provide a mesoporous carbon-silver composite having excellent antibacterial effect, a method for preparing the same, and a water treatment method using the same.

In one aspect of the invention, (a) infiltrating a carbon raw material into the porous silica to obtain a mesoporous silica in which the carbon raw material is deposited; (b) heating and carbonizing the carbonaceous deposited mesoporous silica obtained in step (a) at 700 to 900 ° C. for 1 to 3 hours under a nitrogen atmosphere to obtain a carbide; (c) dissolving mesoporous silica by adding the carbide obtained in step (b) to a 10-30% HF aqueous solution to obtain mesoporous carbon; And (d) mixing the mesoporous carbon obtained in step (c) with an aqueous solution of silver nitrate, and then adding sodium citrate and NaBH ₄ to form a mesoporous carbon-silver complex. A method is provided.

In one embodiment, step (a) is a sulfuric acid is added after mixing the porous silica, sugar and distilled water, it is heated for 4-8 hours at 90 ~ 120 ℃ and then further 4 ~ 8 hours at 130 ~ 200 ℃ It can be carried out by heating for a while to cool to room temperature.

According to another aspect of the present invention, there is provided a mesoporous carbon-silver composite produced by the above production method. In one embodiment, the mesoporous carbon-silver complex may have a diameter of 5 ~ 20 nm of the pupil.

According to another aspect of the invention, there is provided an antimicrobial agent and water treatment agent comprising the mesoporous carbon-silver complex.

According to another aspect of the present invention, there is provided a water treatment method comprising the step of contacting the mesoporous carbon-silver complex with water.

According to the production method of the present invention, it is possible to manufacture a composite of structurally aligned MC and Ag through a simple process in a dehydration process (carbonization) using silica and sugar can reduce the cost, produced by the method The mesoporous (porous) carbon-silver complex has effective antibacterial activity against gram-positive MRSA, which is a highly pathogenic bacterium, and gram-negative E. coli and S. typhi , and thus effective sterilization for drinking water where microbial contamination is a problem. In addition to showing a purification effect and excellent durability in water, it was confirmed that it can be used as an environmentally friendly water treatment agent because it can be washed and dried and recycled after use.

Therefore, the method for producing the mesoporous carbon-silver complex of the present invention, the mesoporous carbon-silver complex produced by the same, and the water treatment method using the same are sterilization and purification of water infected by water-borne pathogens in the field of antibacterial agents, water treatment agents and water treatment methods. It can be usefully used as an effective water treatment agent for.

1 shows electron microscopy (TEM) images of (a) MS (cross section), (b) MC (side), (c) MS-Ag (cross section), and (d) MC-Ag (side), each accumulated The size of the ruler is 100 nm.
Figure 2 schematically shows the test procedure for the antibacterial effect of the mesoporous complex. After co-culturing the mesoporous complex and bacterial cells, the bacteria were plated in Mueller Hinton agar (MHA) medium to calculate the colony forming unit (CFU).
3 shows antibacterial test results (A) for MRSA, E. coli and S. typhi conducted using (a) MS, (b) MC, (c) MS-Ag, and (d) MC-Ag. The graph (B) which measured and showed the number of colony forming units (CFU) by the said test is shown. The data are the results of four separate experiments and the numerical values are expressed as mean ± SEM (* P <0.05, *** P <0.001).

The present invention comprises the steps of (a) infiltrating the carbon raw material into the porous silica to obtain a mesoporous silica in which the carbon raw material is deposited; (b) heating and carbonizing the carbonaceous deposited mesoporous silica obtained in step (a) at 700 to 900 ° C. for 1 to 3 hours under a nitrogen atmosphere to obtain a carbide; (c) dissolving mesoporous silica by adding the carbide obtained in step (b) to a 10-30% HF aqueous solution to obtain mesoporous carbon; And (d) mixing the mesoporous carbon obtained in step (c) with an aqueous solution of silver nitrate, and then adding sodium citrate and NaBH ₄ to form a mesoporous carbon-silver complex. Provide a method.

The production method of the present invention includes the step of infiltrating the carbon raw material into the porous silica to obtain mesoporous silica on which the carbon raw material is deposited (ie, step (a)). Step (a) is followed by mixing sulfuric acid after mixing porous silica, sugar and distilled water, and heating it at 90-120 ° C., preferably at 100 ° C. for 4-8 hours, preferably 6 hours, Further may be carried out by heating to 130 ~ 200 ℃, preferably at 130 ℃, 4 to 8 hours, preferably for 6 hours to cool to room temperature, but is not limited thereto. Step (a) may be repeated within two times to sufficiently fix the carbon.

The production method of the present invention also comprises the step of obtaining a carbide by heating and carbonizing the carbonaceous deposited mesoporous silica obtained in step (a) at 700-900 ° C. for 1 to 3 hours under a nitrogen atmosphere [ie, step ( b)]. Step (b) is a process of carbonizing the carbon raw material deposited in the pores of mesoporous silica at high temperature conditions.

The preparation method of the present invention also includes the step of obtaining the mesoporous carbon by dissolving mesoporous silica by adding the carbide obtained in step (b) in a 10-30% HF aqueous solution (ie, step (c)). Step (c) is a process in which carbonized carbonized mesoporous silica is dissolved in HF aqueous solution to dissolve the silica template, thereby leaving only the mesoporous carbon having an inverse structure. The HF aqueous solution is used at a concentration of 10 to 30% (wt%), preferably 20 to 30% (wt%), more preferably at a concentration of 25% (wt%).

The preparation method of the present invention also comprises mixing the mesoporous carbon obtained in step (c) with an aqueous solution of silver nitrate and then adding sodium citrate and NaBH ₄ to form a mesoporous carbon-silver complex [ie, step (d) ] Is included. Step (d) is a process of dispersing silver nitrate on the mesoporous carbon surface.

In the preparation method of the present invention, as a result of adding silver particles with a support of a mesoporous material having an adsorption effect to prevent aggregation of AgNPs, AgNPs can be dispersed between mesoporous materials without any aggregation phenomenon, and AgNP can be prepared. Since only a small amount of silver precursor is needed, it can reduce the toxicity that can be caused by silver and lower the manufacturing cost. In addition, since the mesoporous carbon-silver nanocomposite is prepared using sugar as a carbon raw material and a small amount of silver nitrate, the manufacturing cost is low and does not include a complicated step.

The present invention also provides a mesoporous carbon-silver composite prepared by the above production method.

In one embodiment, at the time of preparation of the mesoporous carbon-silver complex, 5.4 mg of silver per 50 mg of mesoporous carbon can be added (in this case, about 90% carbon, 9.7% silver), and the final product is filtered and In the final stage of drying, the silver (not to be attached to the mesoporous carbon) can be removed, so the actual silver content (wt%) can be 9.7 wt% or less.

The mesoporous carbon-silver composite has a mesoporous carbon structure of amorphous graphite and is stable even in hot water unless it is heated for a long time at a high temperature of 100 degrees Celsius or more in air. Can be. Therefore, the mesoporous carbon-silver nanocomposite is easy to handle and store, and has excellent physical properties that can be dried after manufacture and stored in a container with a lid.

In one embodiment, the mesoporous carbon-silver composite may have a diameter of 5 ~ 20 nm, preferably 9 ~ 10 nm of the pupil, but is not limited thereto.

Since the mesoporous carbon of the present invention has a small, uniform, and regular arrangement of pores, and may act as a molecular sieve, bacteria larger in size than most of the pores (most bacteria are larger than the pore size) The mesoporous carbon-silver can not pass through the nanocomposite and remains filtered out of the composite, and the antibacterial effect can be enhanced by the synergistic action of carbon and silver nanoparticles in the composite.

The present invention also provides an antimicrobial agent comprising the mesoporous carbon-silver complex produced by the above production method.

In the antimicrobial test, the mesoporous carbon-silver complex may be used at 10 μg per 10 ml of medium, and the test may be performed for a time sufficient to exhibit antimicrobial activity, for example, 18 hours.

In one embodiment, the mesoporous carbon-silver complex may have antibacterial activity against gram negative and gram positive bacteria, but is not limited thereto.

Silver nanoparticles have been known to have antimicrobial activity by the principle of reacting with and damaging bacterial cell membranes or DNA, but tended to aggregate into agglomerates. Mesoporous carbon itself has a large surface area to adsorb microorganisms, and serves as a molecular sieve to trap the microorganisms in the pupil, as well as a support for the dispersion of silver nanoparticles. In the mesoporous carbon-silver composite of the present invention, silver nanoparticles are adsorbed on the surface of mesoporous carbon, and the advantages of mesoporous carbon and silver nanoparticles are combined to obtain a synergistic antibacterial effect.

The Gram-positive bacteria include the antibiotic resistant Gram-positive bacteria, methicillin resistant Staphylococcus aureus (MRSA), and Gram-negative bacteria include, for example, E. coli and Salmonella typhi ( S. typhi ), but is not limited thereto and may include various waterborne pathogens.

The present invention also provides a water treatment agent comprising the mesoporous carbon-silver composite produced by the above production method.

Since the complex in which silver nanoparticles are dispersed in a regularly arranged porous carbon shows excellent antimicrobial activity against various water-borne bacteria, the complex of the present invention can be used for sterilization and purification of drinking water to prevent food poisoning. In addition, since the composite of the present invention uses porous carbon, it is durable in heat or water of 100 degrees Celsius or less in air, and a small amount of silver can be used to provide an environmentally friendly water treatment agent.

The antibacterial agent for water treatment of the present invention is used for water treatment for water in which microbial contamination is a problem, for example, a filter medium for a water purifier, a water tank for a water server, a circulating water, a cut flower water, a water pipe, a tank, a pool or a pond. In the refrigerator, ice water, humidifier, air conditioner drain water, etc. can be effectively used, but is not limited thereto.

The present invention also provides a water treatment method comprising the step of contacting the mesoporous carbon-silver composite produced by the above production method with water.

In one embodiment, the complex is added at 0.001 to 1000 g per kg of water, and may be treated for 10 to 30 hours, preferably 18 hours, but is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to Examples. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention.

<Example> Mesoporous  Preparation of the complex

In order to obtain a high antimicrobial mesoporous complex, mesoporous silica (MS, Comparative Example 1), mesoporous carbon (MC, Comparative Example 2), mesoporous silica-silver complex (MS-Ag, Comparative Example 3) and meso Porous carbon-silver complexes (MC-Ag, Example 1) were each prepared by the following method and their antimicrobial activity was compared.

Mesoporous silica (MS) is a structure-directing agent in 0.8 g of Pluronic P123 surfactant (EO ₂₀ PO ₇₀ EO ₂₀ , Sigma-Aldrich) with 10 ml of distilled water and 10 ml of 1M acetic acid. Add and mix well for 1 hour, and then continue to drop by dropping 2.78 g of sodium silicate solution (Sigma-Aldrich, ~ 27% SiO ₂ , ~ 14% NaOH) dissolved in 30 ml of distilled water. Continue to stir for 30 minutes. The reaction was continued for 20 hours while shaking in a 60 ° C. water bath, then filtered, washed with distilled water and dried at room temperature. The sample was placed in an electric furnace and heated at 550 ° C. for 6 hours, and then cooled slowly to room temperature to prepare hexagonal mesoporous silica (Comparative Example 1).

Mesoporous carbon (MC) has the reverse structure of porous silica. Mix 1 g of porous silica with 1.25 g of sugar and 6 ml of distilled water and stir well while dropping 0.14 g of sulfuric acid. The mixture was heated at 100 ° C. for 6 hours and heated at 160 ° C. for 6 hours, and then slowly lowered to room temperature. To this sample where some carbon raw materials were fixed, a solution containing 0.75 g of sugar and 5 ml of distilled water was added, and 0.08 g of sulfuric acid was added. The sample was placed in a quartz glass tube and heated at 800 ° C. for 2 hours in a nitrogen atmosphere so that the formed carbon was fixed to the silica template. The resulting product was placed in a plastic bottle containing 25% hydrofluoric acid (HF) solution, and then placed overnight, filtered, washed several times with distilled water, and dried to prepare mesoporous carbon in which silica was dissolved (Comparative Example 2).

To prepare the mesoporous silica-silver complex (MS-Ag) (Comparative Example 3) and the mesoporous carbon-silver complex (MC-Ag) (Example 1), distilled water in MS and MC powder (50 mg) in a vial (25 mL) was added respectively and AgNO _{3 (} 8.5 mg, 2 mM) was added to the solution under slow stirring conditions. After 15 minutes, sodium citrate (15 mg, about 2 mM), which acts as a reducing agent and a capping ligand, was added to the solution and stirred for 30 minutes. Next, NaBH _{4 (} 5.7 mg, 6 mM) as a reducing agent was poured into the mixture, followed by continuous stirring for 1 hour, and the mixture was filtered, washed and dried at room temperature.

<Test Example 1> Mesoporous  Complex Antibacterial  Active evaluation

In order to evaluate the antibacterial activity of the mesoporous complexes of Comparative Examples 1, 2, 3 and Example 1, three kinds of bacteria, gram-positive bacteria, methicillin resistant Staphylococcus aureus , MRSA), and Gram-negative bacteria, E. coli and Salmonella typhi , were selected. The bacterium selection criteria is pathogenic causing oral infection, such as food poisoning, in the present invention based on the characteristics of the mesoporous complex, the guidelines of the Clinical and Laboratory Standards Institute (CLSI) The change was used. The minimum inhibitory concentration (MIC) for these three bacteria was determined by modifying the standard protocol of the American Society of Microbiology in the CLSI guidelines.

Since both MC and MC-Ag are black, bacterial growth cannot measure turbidity to the endpoint using conventional optical density (OD) measurements, so the number of bacteria is calculated by a modified MIC test, respectively. The degree of bacterial growth by the nanoparticles of was evaluated.

Specifically, single colonies were separated by a streak plating method, and then transferred to a Muller Hinton medium (MHB, 3 ml) in a sterile state, and then the medium was incubated at 37 ° C. for 18 hours in a shake incubator. . After culturing the medium, the optical density (OD) was measured by spectrophotometer to determine the optimum growth concentration of bacteria. 1 μl of bacteria growing in exponential growth phase were taken and cultured in fresh MHB (1 mL) containing each mesoporous complex (1 mg in dimethyl sulfoxide). An additional culture process was necessary to incubate the bacterial medium at 37 ° C. for 18 hours in a shaker, and the measurement of OD was also performed in the positive control group without the complex of the present invention to confirm the ideal growth rate.

Similar to the method used for the disk-diffusion method, agar plate surfaces were inoculated by plating the same volume of bacterial medium over the entire MHA surface. Bacteria were incubated in each incubator for an additional 18 hours to calculate CFU / mL. Four separate experiments were performed to determine the antibacterial effects of the mesoporous complex, and the data were obtained using the two-tailed Student's t-test or one-way analysis of variance (ANOVA). After comparison, Dunnett's post hoc test was performed using GraphPad Prism software (version 5.01; GraphPad Software). P value <0.05 was considered statistically significant.

<Test Example 2> Mesoporous  Complex Water treatment

The water treatment method performed in this test example included a step of contacting water with the antibacterial treatment agent for water treatment. The use form of the antibacterial treatment agent for water treatment of the present invention can be used by passing water to be treated to a container containing a conventionally used form, for example, an antibacterial treatment agent, but is not particularly limited. The antimicrobial agent does not always need to be present in water, and once it is dried in air, it can be reused since its performance does not change significantly when it is returned to water. However, after use as an antimicrobial agent, the content of silver contained in the mesoporous carbon-silver complex will be reduced, so to maintain the same antimicrobial activity, wash the used complex and then perform the step (d) again with a small amount of silver nitrate solution. do.

The use of the antibacterial agent for water treatment of the present invention is not particularly limited, and can be effectively used for water treatment applications for water in which microbial contamination is a problem. For example, a filter medium for water purifiers, a water tank for water servers, circulating water, cut flowers, water pipes and tanks, water for ice production in a pool or pond, a refrigerator, a humidifier, air conditioner drain water, and the like.

Results and Discussion

MS-Ag / MC-Ag prepared by incorporation of MS / MC and AgNP of the present invention induces physical and chemical damage to bacterial cell membranes through adsorption to MS or MC and membrane penetration by AgNP. In addition, MS or MC serves as a support for the dispersion of AgNPs, preventing AgNPs from aggregation. That is, pure AgNPs tend to aggregate into larger silver particles, which reduces the interaction between the silver particles and the bacterial cell surface, and thus requires a support to disperse and hold the AgNPs separately to prevent aggregation. In addition, in order to manufacture nano-sized AgNPs in order to increase the surface area of the entire silver, very small amount of silver precursors need to be used. In the present invention, MS / MC may serve as a support for silver particles in addition to adsorbing and filtering bacteria. In addition, since a very small amount of silver precursor is required in the preparation of AgNP, it not only reduces the toxicity of silver but also provides the advantage that it can be safely applied clinically with minimal raw material cost.

FIG. 1 shows electron microscopy (TEM) images of MS, MC, MS-Ag and MC-Ag prepared by Comparative Examples and Examples, wherein MS of a regularly arranged hexagonal mesoporous structure (FIG. 1A) is shown. And MC in a replicated form (see FIG. 1 (b)). In the formation of MS-Ag, an aqueous AgNO ₃ solution penetrated into hydrophilic MS, after which the reduced AgNP was dispersed throughout the silica (see FIG. 1 (c)). However, during the formation of MC-Ag, the aqueous AgNO ₃ solution could not penetrate into the hydrophobic MC, and the reduced AgNP was mainly dispersed only on the surface of the MC (see FIG. 1 (d)). Thus, it can be seen that the overall surface area of silver that can contact bacteria when attached to the MC surface is larger than when silver is attached to the MS surface.

The mesoporous complex of the present invention has a small, uniform, and regular arrangement of about 10 nm in size of the pupil, which may serve as a molecular sieve. Thus, bacteria larger in size than most of these pores (most bacteria larger than the pore size) will not be able to pass through the mesoporous carbon-silver nanocomposite and remain filtered out of the complex, where carbon and silver nanoparticles The synergistic effect of can increase the antibacterial effect.

We also investigated antibacterial activity against three highly pathogenic bacteria of MS, MC, MS-Ag and MC-Ag of Comparative Examples 1, 2, 3 and Example 1, wherein the three highly pathogenic bacteria were antibiotic resistant Gram- a negative bacterium Escherichia coli (E. coli) and Salmonella typhimurium (S. typhi) - positive bacteria is methicillin-resistant Staphylococcus aureus (MRSA), grams. MRSA are spherical bacteria (cocci) of about 1 μm in diameter, and E. coli and S. typhi are rounded cylindrical bacteria (bacillus) with an average width of about 1 μm and a length of 2 μm.

3 shows experimental data for characterizing antibacterial properties for each bacterium causing waterborne infections and foodborne diseases of MS, MC, MS-Ag and MC-Ag. As a result, MC-Ag was shown to have antibacterial activity regardless of the type and size of bacteria. Experimental results on antibacterial efficiency are as follows: First, the growth of MRSA is relatively inhibited in the medium of MS and MC, but the antibacterial effect is increased when MC and Ag are combined. Second, the bacteria - gram-negative bacterium Escherichia coli (E. coli) and Salmonella typhimurium (S. typhi)] and the same time of the mesoporous matrix from the culture (co-culture), look that the MS is more effective than MC, wherein The bacterial effect was significantly increased when combining MC and Ag. MS has been reported to cause damage to cell membranes by the hydroxyl groups on the surface interacting with the lipopolysaccharide of the Gram-negative bacterial outer membrane to form hydrogen bonds. Thus, for E. coli and S. typhi bacteria, not only physical damage of bacterial cell membranes through adsorption to MS, but also chemical damage through interaction between hydroxyl group of MS and glycolipid of membrane It is estimated.

Minimal Inhibitory Concentration ( MCC ) of MC-Ag was measured at 1 μg / ml for each strain of MRSA , E. coli , and Salmonella typhi .

Synergistic antibacterial effects were observed to be better in MS-Ag and MC-Ag compared to MS and MC in all media containing bacteria. This suggests that in addition to the physical / chemical damage caused by the mesoporous matrix MS / MC, silver additives play an important role in damaging bacterial cells.

In particular, no growth of bacteria was observed in the medium containing MC-Ag compared to the medium containing MS-Ag, which means that MC-Ag has a very effective antibacterial effect regardless of the type of bacteria. do. This is presumed to be related to the surface area of AgNPs attached to the mesoporous matrix. In other words, AgNPs attached to the MC surface had a larger contact area and smaller particle size than those attached to the MS surface, as shown in the TEM image. Thus, AgNPs on MC will interact with more bacterial cells and are believed to be able to more strongly inhibit bacterial growth than if silver had spread throughout MS. This means that the size of the silver additive, which determines the total contact area and the degree of dispersion attached to the matrix surface, is also an important factor influencing the synergistic effect of the antibacterial activity.

As such, the present inventors identified the mesoporous carbon-silver complex having the highest antibacterial effect among the mesoporous complexes prepared in Comparative Examples 1, 2, 3 and Example 1, and used it for water treatment for drinking water, which is a problem of microbial contamination. To provide. The mesoporous carbon-silver composite is an amorphous graphite molecule, which is stable in hot water unless it is heated for a long time at a high temperature of more than 100 degrees Celsius in air. have. In addition, the mesoporous carbon-silver nanocomposite is easy to handle and store, and has excellent physical properties that can be dried after manufacture and stored in a container with a lid. Therefore, the mesoporous carbon-silver complex of the present invention exhibits excellent physical properties as well as strong antibacterial activity, and thus may be used as an effective water treatment agent.

Claims (7)

(a) After mixing the hexagonal porous silica, carbon raw material and distilled water, sulfuric acid is added, and it is heated at 90-120 ° C. for 4-8 hours and further heated at 130-200 ° C. for 4-8 hours. Cooling to room temperature to obtain mesoporous silica on which carbon material is deposited;
(b) heating and carbonizing the carbonaceous deposited mesoporous silica obtained in step (a) at 700 to 900 ° C. for 1 to 3 hours under a nitrogen atmosphere to obtain a carbide;
(c) adding the carbide obtained in step (b) to a 10-30% HF aqueous solution to dissolve mesoporous silica to obtain mesoporous carbon having an aligned structure; And
(d) mixing the mesoporous carbon obtained in step (c) with an aqueous solution of silver nitrate and then adding sodium citrate and NaBH ₄ to form a mesoporous carbon-silver complex.
Method for producing a mesoporous carbon-silver composite comprising a. The method of claim 1, wherein step (a) is a mixture of porous silica, sugar and distilled water, and then sulfuric acid is added, which is heated at 90 to 120 ° C. for 4 to 8 hours, and then further at 4 to 8 at 130 to 200 ° C. It is carried out by heating for a period of time to cool to room temperature. The mesoporous carbon-silver composite prepared by claim 1. The mesoporous carbon-silver composite according to claim 3, wherein the pupil has a diameter of 5 to 20 nm. An antimicrobial agent comprising the mesoporous carbon-silver complex of claim 3. A water treatment agent comprising the mesoporous carbon-silver complex of claim 3. A method of treating water comprising contacting the mesoporous carbon-silver complex of claim 3 with water.

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