KR102007933B1 - Thermophilic bacterial consortium and method for simultaneous degradation of methane and compounds inducing odor at high temperature using the same - Google Patents

Abstract

The present invention is a mixed strain for the high-temperature decomposition of methane and odor-inducing compounds, a composition for the simultaneous high-temperature decomposition of methane and odor-inducing compounds comprising the mixed strain or a culture thereof as an active ingredient, and a method for the simultaneous high-temperature decomposition of methane and odor-inducing compounds using the same It is about.
The thermophilic bacterial consortium according to the present invention exhibits very high methane and odor removal efficiency inside the biocover which has become a high temperature environment by hot landfill gas and high atmospheric temperature. The high temperature bacterial consortium of the present invention is not only a high temperature landfill gas, but also a simultaneous treatment of methane and odor-inducing compounds of biogas discharged under high temperature conditions such as a high temperature anaerobic fermenter of organic waste, a composting device of organic waste, and a landfill of animal carcasses. It can be used as a useful microbial source.

Description

Thermophilic bacterial consortium and method for simultaneous degradation of methane and compounds inducing odor at high temperature using the same}

The present invention is a mixed strain for the high-temperature decomposition of methane and odor-inducing compounds, a composition for the simultaneous high-temperature decomposition of methane and odor-inducing compounds comprising the mixed strain or a culture thereof as an active ingredient, and a method for the simultaneous high-temperature decomposition of methane and odor-inducing compounds using the same It is about.

Under anaerobic conditions, organic matter is decomposed by microorganisms and finally decomposed into methane and carbon dioxide. Odor-inducing compounds such as hydrogen sulfide, methyl mercaptan, methyl sulfide, ammonia, amines and fatty acids are generated during this decomposition. Organic waste treatment facilities and processes such as landfills, food waste treatment facilities, anaerobic digesters, and barns are the representative sources of methane and odor emissions at the same time. The amount of methane in landfills is 35 ~ 73 teragrams (Tg) per year. It is estimated.

To date, odor management for organic waste treatment facilities (processes), such as landfills, has been focused on odor measurement and monitoring. In the future, it will be mandatory to report the installation of odor emission facilities for public environmental facilities such as landfills, manure / livestock manure treatment facilities and other organic waste treatment facilities. Accordingly, there is an increasing need to develop technologies for removing methane and odor generated from waste treatment facilities (processes) such as landfills.

Biological methods using biocovers (open systems) and biofilters (closed systems) can reduce methane emissions from aged landfills or small landfills that are too low for methane to be recovered and resourced, or that do not have gas collection wells installed. This is a useful way to do it. Methane oxidizing bacteria, which use methane as the only carbon and energy source, play a very important role in oxidizing and removing methane in these biological methods.

On the other hand, since most of the methane oxidizing bacteria are mesophilic, it is known that their performance is best when the temperature of the biofilter or the biocover filler is at a medium temperature (25 to 36 ° C.). However, the landfill gas itself is a high temperature of about 40 ℃, especially in the summer, the temperature of the biocover for treating the landfill gas rises to 45 ℃, high 50 ~ 60 ℃, to remove methane and odor-causing compounds in high temperature environment There is a need for method development.

Therefore, the present inventors have tried to develop a method for decomposing methane and odor-inducing compounds in a high temperature environment, the result is a high temperature obtained by thickening and culturing at 50 ℃ while supplying methane and DMS as the only carbon source as compost source The bacterial consortium was found to effectively co-decompose methane and malodorous compounds at high temperatures, thus completing the present invention.

Accordingly, the present invention includes Brevibacillus thermoruber, Actinomadura rubrobrunea, Amycolatopsis thermalba and Inquilinus ginsengisoli. An object of the present invention is to provide a mixed strain for simultaneous high temperature decomposition of methane and malodor-causing compounds.

In addition, the present invention is a composition for simultaneous high-temperature decomposition of methane and malodor-causing compounds comprising the mixed strain or its culture as an active ingredient, a biocover or biofilter comprising the same and a simultaneous high temperature decomposition system comprising the biocover or biofilter The purpose is to provide.

In addition, the present invention comprises the steps of treating the composition to methane and odor-causing compounds source; And it is an object of the present invention to provide a high temperature decomposition method of methane and malodorous compounds comprising the step of decomposing methane and malodorous compounds.

In order to achieve the above object, the present invention is Brevibacillus thermoruber (Actromadura rubrobrunea), Amycolatopsis thermalba (Amycolatopsis thermalba) and Inquilinus ginseng Provided are mixed strains for simultaneous high-temperature decomposition of methane and malodor-causing compounds, including soli (Inquilinus ginsengisoli).

In another aspect, the present invention provides a composition for simultaneous high-temperature decomposition of methane and odor-inducing compounds comprising the mixed strain or its culture as an active ingredient.

In addition, the present invention provides a biocover for high-temperature decomposition of methane and odor-inducing compounds comprising the composition.

In addition, the present invention provides a biofilter for high-temperature decomposition of methane and odor-inducing compounds comprising the composition.

In addition, the present invention comprises the steps of treating the composition to methane and odor-causing compounds source; And the composition decomposes methane and malodor-causing compounds.

In addition, the present invention provides a high temperature decomposition system of the methane and odor-causing compounds including the biocover or biofilter.

The composition for simultaneous high-temperature decomposition of methane and malodor-causing compounds according to the present invention shows very high methane and malodor removal efficiency inside the biocover which has become a high temperature environment by high temperature landfill gas and high atmospheric temperature. The high temperature bacterial consortium of the present invention is not only a high temperature landfill gas, but also a simultaneous treatment of methane and odor-inducing compounds of biogas discharged under high temperature conditions such as a high temperature anaerobic fermenter of organic waste, a composting device of organic waste, and a landfill of animal carcasses. It can be used as a useful microbial source.

1 is a time-phase graph of methane and DMS resolution by a high temperature bacterial consortium at 50 ℃ conditions.
Figure 2 shows the time-phase graph of methane and DMS resolution by high temperature bacterial consortium at 40 ℃ conditions.

Hereinafter, the present invention will be described in more detail.

The present invention is a methane comprising Brevibacillus thermoruber, Actinomadura rubrobrunea, Amycolatopsis thermalba and Inquilinus ginsengisoli. And it provides a mixed strain for the simultaneous high temperature decomposition of malodor-causing compounds.

In addition, the mixed strain of the present invention may further include Hyphomicrobium aestuarii or Chitinophaga sp. Strain to increase the decomposition efficiency of methane and malodor-causing compounds.

In one embodiment, the present inventors used a compost made from food waste as an inoculum, and concentrated the culture at 50 ° C. while supplying methane and DMS as the only carbon source to identify strains constituting a consortium of high-temperature methane and malodor-causing compounds. . As a result of the composition analysis of the bacterial consortium, Brevibacillus thermorubber occupies the most 31% of the total composition, the most dominant, Actinomadura rubrobrunnea (24%), Amycotopis thermal bar (16%), Inquil Linus Ginseng Soli (12%), Hypomicrobium Estuary (8%), Kitinopa genus (5%), and other strains (4%) in order to form a consortium, through which the Brevi Bacillus thermorubber, Actinmadura rubrobrunnea, Amicollapsis thermal bar, Inquilinus ginseng soli, Hyphomicrobium aestuarii and chitinopa are genus strains of methane and odor-inducing compounds It was confirmed that it can be utilized for high temperature decomposition.

Accordingly, the present invention provides a composition for simultaneous high temperature decomposition of methane and malodor-causing compounds comprising the mixed strain or its culture as an active ingredient. The 'culture medium' refers to the culture by inoculating the strain into the liquid medium, the culture solution contains the strain according to the invention. In addition, the culture solution may include an extract of the culture solution or a concentrate of the culture solution.

The composition of the present invention shows very high methane and odor removal efficiency inside the biocover which has become a high temperature environment due to the high temperature of the landfill gas and high ambient temperature, simultaneously the methane and odor-inducing compounds of the biogas discharged at high temperature conditions It can be useful for processing.

In the present invention, the odor-inducing compound is a substance that stimulates the sense of smell of animals including humans and makes them feel unpleasant, and odors cause loss of appetite, difficulty breathing, and cause nausea and vomiting, resulting in confusion of the mind. It is known.

The malodorous compounds are not limited thereto, but are not limited to ammonia, methyl mercaptan, hydrogen sulfide, dimethyl sulfide, dimethyl disulfide, trimethylamine, acetaldehyde, styrene, propionaldehyde, butyraldehyde, n-valeraldehyde, i-valeraldehyde, toluene, benzene, xylene, methyl ethyl ketone, methyl isobutyrate ketone and butyrate acetate may be any one or more compounds selected from the group, preferably hydrogen sulfide, methyl mercaptan, methyl sulfide, It may be any one or more compounds selected from the group consisting of benzene and toluene.

In addition, the composition of the present invention may further comprise a conventional methane oxidizing bacteria in order to increase the decomposition efficiency of methane. The methane oxidizing bacteria include methylomonas, methylomicrobium, methylobacter, methyllocaldum, methylophaga, methyllophasin Methylosarcina, Methylothermus, Methylohalobius, Methylosphaera, Methylocystis, Methylocella, Methyl Locapas (Methylocapsa), methylosinus (Methylosinus), methylococcus (Methylococcus) and the like may be included alone or two or more.

In the present invention, the composition may be characterized by simultaneously decomposing methane and malodorous compounds at high temperature conditions, that is, 40 to 70 ℃. Conventional microorganisms are mesophilic, but the composition for simultaneous high-temperature decomposition of methane and malodorous compounds according to the present invention has high industrial utility from the methane and malodorous compounds at the high temperature of 40 to 70 ℃.

In addition, the present invention provides a biocover for high-temperature decomposition of methane and odor-causing compounds comprising the composition of the present invention.

When the composition of the present invention is put in a bio cover for decomposing methane or malodor-causing compounds, and the biocover is installed in a source of methane or malodor-causing compounds and contacted with methane or malodor-causing compounds, the methane or malodorous compounds are effectively decomposed in a high temperature environment. can do.

In addition, the present invention provides a biofilter for high-temperature decomposition of methane and malodor-causing compounds comprising the composition of the present invention.

The composition of the present invention may be put in a carrier in a biofilter, and the biofilter may be installed in a source of methane or malodor-causing compounds to filter methane or malodor-causing compounds to effectively decompose methane or malodorous compounds in a high temperature environment.

The present invention also provides a method for co-decomposing methane or malodorous compounds using the compositions of the present invention. Specifically, the decomposition method comprises the steps of treating the composition of the present invention to methane and odor-causing compounds source; And the composition decomposes methane and malodor-causing compounds.

The methane or malodor-causing compound source is not limited to the place where methane or malodorous-causing compounds occur, waste landfill, landfill, wastewater treatment plant, manure treatment plant, livestock wastewater treatment plant, food waste treatment plant, petrochemical production plant, It may be a domestic sewage treatment plant, an industrial wastewater treatment plant, a livestock farm, a food processing plant, a paint manufacturing plant, a casting manufacturing plant, a petroleum refining plant, a manure treatment plant, a slaughterhouse, a fertilizer manufacturing plant, a plastic manufacturing combustion plant, a painting plant, or a plating plant. .

The present invention also provides a biocover layer comprising one or more biocover layers; And a ventilation layer surrounding the biocover layer or a ventilation layer stacked below the biocover layer. It is possible to biologically decompose the methane and odor causing compounds by installing a co-decomposition system including the biocover layer, a ventilation layer surrounding it, and a ventilation layer stacked below.

The present invention also provides a biofilter layer comprising one or more biofilters stacked thereon; And a ventilation layer surrounding the biofilter layer or a ventilation layer laminated under the biofilter layer. By installing a simultaneous decomposition system including the biofilter layer, a ventilation layer surrounding it, and a ventilation layer stacked below, the methane and odor-causing compounds can be biologically decomposed.

The biocover layer and the biofilter layer may include the composition of the present invention to biologically decompose methane or malodor-causing compounds in a high temperature environment.

The biocover layer and the biofilter layer may further include the above-described conventional methane oxidizing bacteria.

The biocover layer and the biofilter layer are surrounded by a ventilation layer for supplying oxygen, and the components constituting the ventilation layer are not particularly limited as long as they have a particle size capable of supplying oxygen. For example, it may consist of sand or gravel. In addition, the ventilating layer may be provided with one or more vent pipes for supplying air, it is possible to inject air to the blower through the vent pipe.

Hereinafter, the present invention will be described in detail by way of examples. However, the following examples are merely to illustrate the present invention is not limited to the contents of the present invention.

Example  1. High Temperature Degrading Bacteria Simultaneously with Methane and Odor-causing Compounds Consortium  Secured and active

1-1. Badge composition

NMS medium was used for strain culture, the medium composition is shown in Table 1 and Table 2. Nitrogen concentrate and phosphorus concentrate were prepared to prevent nutrient depletion by repeating the culture, the composition is shown in Table 3.

NMS Medium Composition Components Sample amount (g / L) MgSO _{4 7} H ₂ O 1.0 CaCl ₂ · 6H ₂ O 0.2 KNO ₃ 1.0 KH ₂ PO ₄ 0.272 Na ₂ HPO ₄ · 12H ₂ O 0.717 Trace element 0.5 mL / L 3,000 μM Cu ^{2 +} solution 10 mL / L

Trace element composition Components Sample amount (mg / L) FeSO ₄ 7H ₂ O 200 ZnSO ₄ · 7H ₂ O 10 MnCl ₂ 4H ₂ O 3 H ₃ BO ₃ 30 CoCl ₂ · 6H ₂ O 20 CaCl ₂ · 6H ₂ O One NiCl ₂ · 6H ₂ O 2 Na ₂ MnO ₄ 2H ₂ O 3

Nitrogen, phosphorus concentrate composition Concentrate Type Components Sample amount (g / L) Nitrogen Concentrate KNO ₃ 2.0 Phosphorus Concentrate KH ₂ PO ₄ 0.52 Na ₂ HPO ₄ · 12H ₂ O 1.65

1-2. High Temperature Degrading Bacteria with Methane and Odor-causing Compounds Consortium  culture

To obtain a high-temperature decomposition bacterial consortium with methane and malodorous compounds simultaneously, compost made from food waste was used as inoculum. After mixing 2 g of compost and 20 mL of NMS medium in a 600 mL serum bottle, 50,000 ppm of methane and 5,000 ppm of DMS (dimethyl sulfide, methyl sulfide) were added thereto. Serum bottles were sealed with rubber stoppers and parafilm. Thereafter, in order to prevent the rubber stopper from being opened by increasing the gas volume in the serum bottle by high temperature culture, 80 mL of the mixed gas was removed with a syringe, and the culture was shaken at 50 ° C. and 150 rpm. The gas at the top of the serum bottle was collected with a syringe and the concentration of methane and DMS was measured by gas chromatography. When both methane and DMS were decomposed, the serum bottle was opened, air replaced for 1 hour, and 50,000 ppm of methane and 5,000 ppm of DMS were reinjected. To prevent nutrient depletion, 1 mL of nitrogen and phosphorus concentrate was added every three times of air replacement. The strain was increased by mixing 10 mL of the methane and DMS degrading activity and 10 mL of NMS medium into a new 600 mL serum bottle. The increase was repeated 10 times to secure a high temperature decomposition bacterial consortium with methane and malodor-causing compounds. Table 4 shows the bacterial composition of methane and malodor-causing compounds obtained by the above-described process at the same time.

Composition of High Temperature Degrading Bacteria Consortium with Methane and Odor-causing Compounds Bacteria name ratio( % ) Key features Brevibacillus  thermoruber 31 Gram-positive bacteria, motility and spore forming thermophilic bacteria, aerobic bacteria, Exopolysaccharide producing bacteria Actinomadura  rubrobrunea 24 Gram-positive bacteria, aerobic bacteria without movement, thermophilic bacteria that do not form spores Amycolatopsis  thermalba 16 Gram-positive bacteria, actinomycetes with high G + C content, thermophilic bacteria Inquilinus  ginsengisoli 12 Gram-negative bacteria, bacilli that do not form spores, aerobic bacteria Hyphomicrobium  aestuarii 8 Gram-negative bacteria, bacilli that do not form spores, aerobic bacteria Chitinophaga sp . 5 Gram-negative bacteria, bacilli that do not form spores, aerobic bacteria Etc 4 - Sum 100 -

Example  2. Simultaneous high temperature decomposition of methane and odor-causing compounds Consortium  Active evaluation

In order to evaluate the ability to remove DMS, which is a kind of methane and malodor-causing compounds of the bacterial consortium according to Example 1, the following experiment was performed. In a 600 mL serum bottle, 2 mL of methane and malodor-causing compounds were simultaneously mixed with 18 mL of NMS medium, followed by adding 50,000 ppm of methane and 5,000 ppm of DMS (dimethyl sulfide, methyl sulfide). After sealing the serum bottle with a rubber stopper and a parafilm, 80 mL of the mixed gas was removed by a syringe to prevent the rubber stopper from opening due to an increase in gas volume in the serum bottle by high temperature culture. Then, shaking culture at 50 ℃ and 40 ℃, 150 rpm conditions. The gas at the top of the serum bottle was collected with a syringe and the methane and DMS concentrations were measured by gas chromatography. The results are shown in FIGS. 1 and 2.

As shown in FIG. 1, when 5000 ppm of methane and DMS were repeatedly injected three times at 50 ° C., the rate of decomposition of methane and DMS was slightly increased as the number of repetitions increased. Methane and odor co-decomposed bacterial methane and DMS degrading enzymes are sufficiently expressed that the degradation rate is fast. Finally, it was confirmed that the bacterial consortium of the present invention can decompose methane (50,000 ppm) and DMS (5,000 ppm) within about 75 hours at 50 ° C.

In addition, as shown in Figure 2, when the injection of methane and DMS 5,000ppm three times at 40 ℃ conditions, it was confirmed that the decomposition rate of the methane and DMS was significantly faster as the number of repetitions increased. Finally, it was confirmed that the bacterial consortium of the present invention can decompose methane (50,000 ppm) and DMS (5,000 ppm) within about 50 hours at 40 ° C.

Accordingly, it was confirmed that the bacterial consortium according to the present invention can effectively decompose methane and odors even under high temperature conditions and thus can be usefully used for landfill gas treatment in actual high temperature environments.

Example  3. Simultaneous High Temperature Degrading Bacteria of Methane and Odor-Inducing Compounds of the Present Invention Consortium  Bio cover performance evaluation

3-1. Experiment Method-Bio Cover Installation

In order to determine the ability to remove methane and malodor-causing compounds of the bacterial consortium according to Example 1, a biocover was manufactured and installed in a landfill facility of Gwangyang city.

Specifically, a site having a width of 2 m and a length of 2 m was prepared at a landfill site, and after excavating the waste embedded to a depth of 1 m using an excavator, a biocover outer wall frame was installed using a styrofoam having a thickness of 10 cm. Thereafter, one hole 20 cm in diameter was installed, and the gravel was formed to a height of 0.25 m to prevent the clogging of the hole and the landfill gas well dispersed into the biocover layer, and then the gas collecting pipe was connected to the hole. The gas collection pipe was used for collecting the biocover inlet gas to analyze the concentration of methane and odors introduced into the biocover.

A nonwoven fabric was laid on the gravel layer, and the soil was filled up to 0.25 m in height. On top of the soil layer, a mixture of soil, perlite, compost, hot methane and malodor-causing compounds co-decomposed bacterial consortium culture (soil: pearlite: compost: bacterial consortium = 400: 100: 10: 1, volume ratio) was charged to a height of 0.5 m layer.

In order to analyze the concentration of methane and odor-causing compounds entering the biocover, a gas collecting tube was installed from the bottom of the biocover to the ground portion. In addition, in order to analyze the concentration of methane and odor-causing compounds emitted from the surface of the biocover, a static flux chamber (1.5 x 1.0 x 0.1 m, acrylic) for gas collection was installed on the upper surface of the biocover.

3-2. Experimental method-Analysis method of collected gas

Odor analysis of gas collected at the site was analyzed according to the odor pollution process test method of the Ministry of Environment using the analysis equipment shown in [Table 5]. In order to measure complex odor, using a Tedlar bag (5 L, Tokyo Corporation, Japan) washed three times or more with high-purity nitrogen (99.999%) by vacuum-type lung sampling, at a rate of 1 L / min 5 Samples were taken for -min. Compound odor is an air dilution functional method, in which odorless air prepared by odorless air production device (general air passes through activated carbon and absorbent) is injected into the odorless sample step by step to prepare a diluted sample, and then presented in the odor process test method. Dilution factor was calculated according to the method.

Sampling Method and Analysis Equipment by Type of Odor Substance Items Sampling Analytical instrument Complex odor Dilution value Lung sampler Air dilution method,
Korea Ammonia Ammonia Solution absorption UV / VIS (Shimadzu, Japan) Sulfur Hydrogen sulfide,
Methyl mercaptane
Dimethyl sulfide
Dimethyl disulfide Lung sampler GC / FPD plus TD
(Shimadzu, Japan) Aldehydes Acetaldehyde,
Propionaldehyde,
Butyraldehyde,
n-Valeraldehyde,
iso-Valeraidehyde, DNPH cartridge HPLC / UV
(Younglin, Korea) Amine Trimethylamine Sulfuric acid
absorption GC / NPD
(Shimadzu, Japan) Hydrocarbons
(VOCs) Styrene, Toluene, Xylene,
Methyl ethyl keton, Methyl isobutyl keton, Butyl acetate,
iso-Butyl alcohol Tenax tube
adsorption GC / MSD
(Agilent, USA) Fatty acids Propionic acid,
n-Butyric acid,
n-Valeric acid,
i-Valeric acid KOH solution
filter absorption GC / FID
(Shimadzu, Japan)

The methane analysis was repeated 2 ~ 3 times in the field using a portable measuring device (model: biogas check analyzer, UK), and the gas analysis was performed by GC / FID in the analysis room after collecting gas into the tether bag for precise analysis. Methane analysis conditions are shown in Table 6.

Methane analysis conditions division Condition GC Shimadzu 17A, Japan Detector FID Column MS-5A column (30 m × 2.60 mm × 1 μm) Column temp. 40 ℃ (2 min) → 80 ℃ (10 min) → 200 ℃ (5 min) Injector temp. 250 ℃ Detector temp. 250 ℃ Carrier gas He (99.999%), 40 ml / min Oven Temp. 60 ° C (30min)

3-3. Experimental Results-Evaluation of High Density Landfill Gas Treatment Performance

Table 7 shows the results of evaluating the high concentration of landfill gas treatment performance at high temperature conditions by the biocover inoculated with methane and malodor-causing compounds at the same time high temperature decomposition bacterial consortium.

Removal of methane and odor in high concentration landfill gas by bio cover inoculated with high temperature decomposition bacteria consortium Bio cover temperature 45.2 ~ 49.5 ^o C methane Measuring point density( % ) Removal rate ( % ) Entrance 54.8 98.3
exit 0.9 Odorous substance Measuring point Concentration ( ppb ) Removal rate ( % ) Compound odor Air dilution (fold) Entrance 30,000 93.1
exit 2,080 Nitrogen compounds ammonia Entrance 938.45 46.0
exit 506.50 Trimethylamine Entrance 0.56 -
exit 4.15 Sulfur compounds Hydrogen sulfide Entrance 23,527.81 93.9
exit 1,426.78 Methyl mercaptan Entrance 185.69 76.7
exit 43.19 Dimethyl sulfide Entrance 77.08 7.2
exit 71.52 Dimethyl disulfide Entrance 38.95 -
exit 56.48 volatility
Organic compounds Acetaldehyde Entrance 13.69 72.2
exit 3.81 Propionaldehyde Entrance 0.00 -
exit 0.00 Butylaldehyde Entrance 1.82 100.0
exit 0.00 i-valeraldehyde Entrance 13.78 100.0
exit 0.00 n-valeraldehyde Entrance 1.38 100.0
exit 0.00 Styrene Entrance 70.96 65.8
exit 24.28 toluene Entrance 1,890.57 97.3
exit 51.61 Xylene (m + p + o) Entrance 1,323.53 88.4
exit 153.56 Methyl ethyl ketone Entrance 24.88 100.0
exit 0.00 Methyl Isobutyl Ketone Entrance 37.37 100.0
exit 0.00 Butyl Acetate Entrance 5.28 58.8
exit 2.18 i-butyl alcohol Entrance 3.30 100.0
exit 0.00 Propionic acid Entrance 0.00 -
exit 0.00 n-butyl acid Entrance 50.21 100.0
exit 0.00 i-valeric acid Entrance 0.00 -
exit 0.00 n-valeric acid Entrance 0.00 -

As shown in Table 7, the internal temperature of the biocover was high temperature in the range of 45.2 ° C to 49.5 ° C due to the high temperature of the landfill gas and the high atmospheric temperature. In this condition, the concentration of methane introduced into the biocover was 54.8%, but the concentration of methane released from the biocover was 0.9%, indicating a very high methane removal efficiency of 98.3%.

In addition, the mixed odor of the landfill gas flowing into the biocover was 30,000 times in the air dilution multiple, while the composite odor of the gas discharged from the biocover was 2,080 times in the air dilution multiple. The compound odor removal efficiency by the biocover was found to be very good as 93.1%.

In addition, the analysis of concentration and removal efficiency of each of the 22 odorous substances regulated by Korea's odor prevention law showed that butylaldehyde, i-valeraldehyde, n-valedaldehyde, methyl ethyl ketone, methyl isobutyl ketone, and i- It was confirmed that the removal efficiency of butyl alcohol and n-butyl acid was 100%. The highest concentration of hydrogen sulfide (inflow concentration 23,527.81ppb) among 22 odorous substances was confirmed to be 93.9%. The removal efficiency of toluene having an inflow concentration of 1,890.57 ppb was 97.3% and that of xylene (inflow concentration of 1,323.53 ppb) was 88.4%. The removal efficiency of ammonia, a nitrogen-based odorous substance, was 46%, but trimethylamine was found to have a higher concentration than the inlet concentration. Dimethyl disulfide also had a slightly higher emission concentration than the influent concentration, but the higher concentrations of odorous substances than the influent concentrations are common in landfill sites, and the increased concentration is not so high. Little effect.

3-4. Experimental Results-Evaluation of Low Concentration Landfill Gas Treatment Performance

Table 8 shows the results of evaluating the low concentration landfill gas treatment performance at high temperature conditions by the biocover inoculated with methane and malodor-causing compounds at the same time high temperature decomposition bacterial consortium.

Removal of methane and odor in low concentration landfill gas by bio cover inoculated with methane and odor-inducing compound at high temperature decomposition bacteria consortium Bio cover temperature 45.5 ~ 46.1 ^o C methane Measuring point density( % ) Removal rate ( % ) Entrance 29.4 98.6 exit 0.4 Odorous substance Measuring point Concentration ( ppb ) Removal rate ( % ) Compound odor Air dilution (fold) Entrance 300 85.0 exit 45 Nitrogen compounds ammonia Entrance 66.11 - exit 88.45 Trimethylamine Entrance 0.00 - exit 0.27 Sulfur compounds Hydrogen sulfide Entrance 19.02 97.1 exit 0.55 Methyl mercaptan Entrance 33.72 96.9 exit 1.06 Dimethyl sulfide Entrance 29.70 95.1 exit 1.46 Dimethyl disulfide Entrance 14.63 100.0 exit 0.00 volatility
Organic compounds Acetaldehyde Entrance 1.10 - exit 4.83 Propionaldehyde Entrance 0.00 - exit 0.00 Butylaldehyde Entrance 0.00 - exit 0.78 i-valeraldehyde Entrance 3.41 100.0 exit 0.00 n-valeraldehyde Entrance 0.00 - exit 0.00 Styrene Entrance 11.06 82.7 exit 1.91 toluene Entrance 307.44 98.8 exit 3.65 Xylene (m + p + o) Entrance 700.62 98.6 exit 10.00 Methyl ethyl ketone Entrance 0.00 - exit 0.00 Methyl Isobutyl Ketone Entrance 3.15 100.0 exit 0.00 Butyl Acetate Entrance 0.00 - exit 0.00 i-butyl alcohol Entrance 0.00 - exit 0.00 Propionic acid Entrance 0.00 - exit 0.00 n-butyl acid Entrance 87.47 41.3 exit 51.37 i-valeric acid Entrance 0.00 - exit 0.00 n-valeric acid Entrance 0.00 - exit 0.00

The biocover internal temperature was a high temperature condition in the range of 45.5 degreeC-46.1 degreeC. In this condition, the concentration of methane introduced into the biocover was 29.4%, but the concentration of methane released from the biocover was 0.4%, indicating a very high methane removal efficiency of 98.6%.

In addition, the mixed odor of the landfill gas flowing into the biocover was 300 times in the air dilution multiple, but the composite odor of the gas discharged from the biocover was 45 times in the air dilution multiple. The compound odor removal efficiency by the biocover was confirmed to be very good (85.1%).

As a result of analyzing the concentration and removal efficiency of each of the 22 odorous substances regulated by the odor prevention law of Korea, it was confirmed that the removal efficiency of dimethyl disulfide, i-valeraldehyde and methyl isobutyl ketone was 100%. In addition, the removal efficiencies of hydrogen sulfide, methyl mercaptan, dimethyl sulfide, toluene and xylene were very good at 95% or more. The removal efficiencies of styrene and n-butyl acid were 82.7% and 41.3%, respectively. The emission concentrations were slightly higher than the influent concentrations of ammonia, acetaldehyde and butylaldehyde, but had little effect on the complex odor because the increased concentration was not so high.

As can be seen from the above results, it was confirmed that the methane and odor-inducing compounds simultaneously high temperature decomposition bacterial consortium decomposes methane and odor-inducing compounds in landfill gas very efficiently under high temperature conditions of 40-50 ° C.

Therefore, the bacterial consortium of the present invention is not only a high-temperature landfill gas, but also in the simultaneous treatment of methane and odor-causing compounds of biogas discharged under high temperature conditions such as a high temperature anaerobic fermenter of organic waste, a composting device of organic waste, a landfill of animal carcasses, etc. It can be usefully used.

As mentioned above, although preferred embodiment of this invention was described in detail, the technical scope of this invention is not limited to the above-mentioned embodiment, It should be interpreted by the claim. At this time, those of ordinary skill in the art should consider that many modifications and variations are possible without departing from the scope of the present invention.

Claims (11)

Methane and odor induction, including Brevibacillus thermoruber, Actinomadura rubrobrunea, Amycolatopsis thermalba and Inquilinus ginsengisoli Mixed strains for simultaneous high temperature degradation of compounds.
According to claim 1, Hyphomicrobium aestuarii (Hyphomicrobium aestuarii) or chitinpa (Chitinophaga sp.), Characterized in that it further comprises a strain (Chitinophaga sp.), Methane and odor-induced compound mixed strain for simultaneous high temperature degradation.
The composition for simultaneous high temperature decomposition of methane and odor-inducing compounds comprising the mixed strain of claim 1 or a culture thereof as an active ingredient.
According to claim 3, The malodorous compound is methane and malodorous compound, characterized in that any one or more compounds selected from the group consisting of hydrogen sulfide, methyl mercaptan (methanethiol), dimethyl sulfide (benzene sulfide) and toluene Composition for simultaneous high temperature decomposition.
According to claim 3, wherein the high temperature is 40 to 70 ℃, methane and odor-inducing compound at the same time high temperature decomposition composition.
Bio cover for high-temperature decomposition of methane and malodor-causing compounds comprising the composition of claim 3.
Biofilter for high temperature decomposition at the same time methane and malodorous compounds comprising the composition of claim 3.
Treating the composition of claim 3 with a methane and malodor generating compound source; And the composition decomposing methane and malodorous compounds at high temperature.
9. The method of claim 8, wherein the methane and odor-causing compounds are generated from landfills, landfills, wastewater treatment plants, manure treatment plants, livestock wastewater treatment plants, food waste treatment plants, petrochemical production plants, domestic sewage treatment plants, industrial wastewater treatment plants, and livestock farms. High-temperature methane and odor-inducing compounds, characterized in that the food processing plant, paint manufacturing plant, foundry manufacturing plant, petroleum refining plant, manure treatment plant, slaughterhouse, fertilizer plant, plastic manufacturing combustion plant, coating plant or plating plant Decomposition method.
A biocover layer comprising one or more biocovers of claim 6 stacked thereon; And a ventilation layer surrounding the biocover layer or a lower layer of the biocover layer.
A biofilter layer in which at least one biofilter of claim 7 is stacked; And a ventilation layer surrounding the biofilter layer or a lower layer of the biofilter layer.

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