KR102003859B1 - Jig for fine droplet generation - Google Patents

Abstract

TECHNICAL FIELD The present invention relates to a jig for micro-liquid production, and more particularly, to a jig for micro-liquid production for rapidly and precisely producing micro-liquids of uniform size. The structure of the present invention includes a cartridge unit configured to generate microcapsules therein; An upper housing unit provided on an upper portion of the cartridge unit; A lower housing unit provided at a lower portion of the cartridge unit; And a discharging unit coupled to the upper housing unit and discharging the generated micro liquid droplets, wherein the cartridge unit is provided to generate micro liquid droplets by the fluid pressure applied thereto. Provide a jig.

Description

JIG FOR FINE DROPLET GENERATION}

TECHNICAL FIELD The present invention relates to a jig for micro-liquid production, and more particularly, to a jig for micro-liquid production for rapidly and precisely producing micro-liquids of uniform size.

Bacteria, and pathogens are causing many casualties and costs each year. Accordingly, various analytical methods for rapidly and accurately detecting and analyzing pathogens and bacteria have been developed.

Specifically, the microorganism culture method, which is one of the methods for analyzing microorganisms, is a method in which a microorganism is cultured for an extended period of time in an environment capable of proliferation, and then the kind of microorganism is analyzed using a microscope or a staining method. However, such a microorganism culture method requires a skill of skill because it requires an environment in which microorganisms can be propagated and must undergo complicated experimental steps, and it takes a lot of time to analyze microorganisms.

In addition, the immunological reaction method of microorganisms is a method of detecting various specific antigens, single toxins or enzymes produced in a place where a pathogen grows, and has an advantage that it can be applied to a small amount of pathogen. However, such an immune response method does not identify the microorganism itself as an antigen, but refers to an antibody that reflects the presence of the antigen, so that defects that can not reflect the presence of the pathogen due to the time difference until the antibody titer increases .

In addition, the ATP (Adenosine triphosphate) method is a method of measuring light generated during metabolism of living organisms. However, since the ATP method is not a device for directly measuring bacteria, there is a problem that it is difficult to accurately detect the number of microorganisms since the ATP measurement value is not directly proportional to the number of microorganisms.

Recently, a method of culturing a pathogen using PCR-based technology and extracting genes and analyzing the amplification of genes using agarose gel electrophoresis has been mainly used.

However, such an agarose gel electrophoresis method has a problem in that it is visually inspected when the concentration of the pathogen is low, and there is a limitation in actually detecting presence or absence of the pathogen at a low concentration.

Specifically, when the concentration of the pathogen is extremely small, such as when the pathogen concentration is less than 103 cells per solution, even if the pathogen is amplified, the signal for detection is very weak because the pathogen is dispersed. Therefore, there is a problem that it is difficult to accurately and quickly detect the presence or absence of pathogens when the pathogens are extremely small.

In addition, the conventional analysis method using the agarose gel electrophoresis has a limitation in that it is difficult to quantitatively detect pathogens.

Therefore, in recent years, there has been developed a technique for generating micro-droplets so as to detect pathogens more rapidly and quantitatively and detecting and analyzing the genes contained therein.

For example, U.S. Patent No. 9328376 (hereinafter referred to as "the prior art") includes a carrier fluid channel for introducing oil, a sample channel for introducing the sample, and a droplet channel for the generated micro fluid. And, the above-mentioned prior art is prepared to introduce the oil and the sample into the droplet channel, respectively, to produce microcavities.

However, in the conventional technique, only one droplet channel is formed in one microcavity generating apparatus, and a pump is provided in each sample channel and carrier fluid channel. Therefore, in the prior art, the number of pumps to be controlled in proportion to the number of micro-liquid generating apparatuses increases as the micro-liquid generating apparatuses are used in order to quickly produce large volumes of micro liquid, There is a problem that production is difficult.

In addition, since the conventional techniques are controlled by using different pumps in different micro liquid generating devices, there is also a problem that micro liquid substances of different sizes are generated in different micro liquid producing devices due to the difference of the minute flow rates.

In this way, conventionally, it is difficult to rapidly generate a large amount of micro liquid having a uniform size, and control is difficult.

Therefore, there is a need for a technique for rapidly and precisely generating a large amount of microcapsules of uniform size.

United States Patent No. 9328376

SUMMARY OF THE INVENTION An object of the present invention is to provide a jig for micro-liquid production for rapidly and precisely generating a micro liquid having a uniform size.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise form disclosed. There will be.

According to an aspect of the present invention, there is provided a cartridge comprising: a cartridge unit configured to generate microcapsules therein; An upper housing unit provided on an upper portion of the cartridge unit; A lower housing unit provided at a lower portion of the cartridge unit; And a discharging unit coupled to the upper housing unit and discharging the generated micro liquid droplets, wherein the cartridge unit is provided to generate micro liquid droplets by the fluid pressure applied thereto. Provide a jig.

According to an embodiment of the present invention, the cartridge unit may include: a substrate unit having a microchannel formed therein; An oil injection unit provided at an upper portion of the substrate unit and adapted to inject oil into the micro flow path; A sample injecting unit provided at one side of the oil injecting unit and injecting the sample into the micro channel; And a collecting part provided at one side of the sample injecting part and collecting the micro liquid droplets generated in the micro flow path.

In an embodiment of the present invention, the oil injector, the sample injector, and the collecting unit may be provided in plural, and each of the oil injector, the sample injector, and the collecting unit may be provided to form one row on the substrate.

In the embodiment of the present invention, the sample injecting portion and the oil injecting portion are provided to discharge the sample and the oil toward the micro flow path, respectively, by the fluid pressure, and the sample is separated by the oil to form independent fine droplets Of the total amount of the product.

In an embodiment of the present invention, the sample injected into the micro channel by the sample injecting unit may include a PCR reagent.

In an embodiment of the present invention, the upper housing unit includes: an upper housing part coupled to an upper portion of the substrate part; An oil injection port provided in the upper housing part and adapted to be engaged with the oil injection part; A sample inlet provided in the upper housing part and adapted to be coupled to the sample injector; And a collecting outlet provided in the upper housing portion and adapted to engage with the collecting portion.

In an embodiment of the present invention, the upper housing portion may include a center frame provided with the oil inlet, the sample inlet, and the collecting outlet, and positioned at the upper portion of the substrate portion; And a side frame provided at a lower portion of the center frame and extending and extending toward both sides of the center frame.

In an embodiment of the present invention, the lower housing unit may include: an inner frame disposed on an upper portion of the lower housing unit; And an outer frame provided on both sides of the inner frame and adapted to engage with the side frames of the upper housing unit, wherein the outer frame is formed with a step higher than the inner frame, And the base portion is fixed.

In the embodiment of the present invention, the discharging unit may include a stud portion provided to be inserted into the collecting outlet of the upper housing unit and coupled to the lower housing unit, And a tube coupling portion extending from the upper portion of the stud portion, wherein a tube coupling hole through which the tube can be coupled is formed in the tube coupling portion.

In an embodiment of the present invention, the vacuum pump unit further includes a vacuum pump unit connected to the discharge unit, wherein the vacuum pump unit vacuum-sucks the oil and the sample injected into the oil injection unit and the sample injection unit, And the like.

In an embodiment of the present invention, a first pump unit connected to the oil injecting unit and adapted to apply a pump pressure to the oil injecting unit; And a second pump unit connected to the sample injecting unit and adapted to apply a pump pressure to the sample injecting unit, wherein the first pump unit and the second pump unit are respectively connected to the oil injecting unit and the sample injecting unit, And a pump pressure is applied to the oil and the sample injected into the pump to generate the undiluted solution.

In the embodiment of the present invention, the vacuum pump unit may further include a vacuum pump unit connected to the discharge unit, wherein the first pump unit and the second pump unit are respectively connected to the oil injection unit and the sample injection unit And injecting the oil and the sample injected into the oil injecting unit and the sample injecting unit into the vacuum pump to generate microcapsules when the pump pressure is applied to the oil and the sample injected into the oil injecting unit and the sample injecting unit.

In the embodiment of the present invention, it may further comprise a storage unit for collecting and storing the undiluted liquid discharged from the discharge unit.

According to an aspect of the present invention, there is provided a microcavity generating apparatus to which a microcavity generating jig is applied.

The effect of the present invention with the above-described structure can rapidly and massively produce microcapsules of uniform size.

In particular, according to the first embodiment of the present invention, since the flow of all the fluids is controlled by using the vacuum pump unit, all the connected fluids can be moved at the same flow rate. Thus, when the sample and the oil are moved to the same flow rate, uniform microcapsules can be generated in the microchannels in different assemblies.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

1 is an assembled perspective view of a jig for micro-liquid production according to an embodiment of the present invention.
2 is an exploded perspective view of a micro liquid generating jig according to an embodiment of the present invention.
3 is a bottom view of the micro liquid generating jig according to an embodiment of the present invention.
4 is a front view of a microgroovability jig according to an embodiment of the present invention.
5 is a side view of a jig for generating microcracks according to an embodiment of the present invention.
6 is a top view of a jig for generating microcracks according to an embodiment of the present invention.
7 is a cross-sectional view along the line A-A 'in FIG.
8 is a cross-sectional view taken along the line B-B 'in Fig.
9 is a cross-sectional view taken along line C-C 'of FIG.
10 is a sectional view taken along the line D-D 'in FIG.
11 is an exemplary view showing a pump unit of a micro liquid generating jig according to an embodiment of the present invention.
12 is a photograph of a cartridge unit of a micro liquid generating jig according to an embodiment of the present invention.
13 is a photograph showing the coupling of the micro liquid generating jig according to the embodiment of the present invention.
FIG. 14 is a flowchart of a microcavity generation method using a microgass production jig according to an embodiment of the present invention.
FIG. 15 is a diagram illustrating an example of a microcavity generating method using a microcavity generating jig according to the first embodiment of the present invention. FIG.
16 is an exemplary view showing a fluid flow of the micro liquid generating jig according to the first embodiment of the present invention.
17 is a diagram illustrating an example of a micro-liquid generating method using a micro-liquid generating jig according to the second embodiment of the present invention.
18 is an exemplary view of a microcavity generating method using a microcavity generating jig according to the third embodiment of the present invention.
FIG. 19 is a photograph showing microcavities generated according to the microcavity generation method using the microcavity production jig according to an embodiment of the present invention. FIG.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and similar parts are denoted by like reference characters throughout the specification.

Throughout the specification, when a part is referred to as being "connected" (connected, connected, coupled) with another part, it is not only the case where it is "directly connected" "Is included. Also, when an element is referred to as "comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 is an exploded perspective view of a micro-liquid generating jig according to an embodiment of the present invention, and FIG. 2 is an exploded perspective view of a micro-liquid generating jig according to an embodiment of the present invention.

FIG. 3 is a bottom view of a micro liquid generating jig according to an embodiment of the present invention, FIG. 4 is a front view of a micro liquid generating jig according to an embodiment of the present invention, and FIG. Fig. 8 is a side view of the micro liquid generating jig according to the embodiment. Fig.

1 to 5, the micro liquid generating jig 1000 includes a cartridge unit 1100, an upper housing unit 1200, a lower housing unit 1300, a discharge unit 1400, and a coupling unit 1500, And the cartridge unit 1100 may be provided to generate microcavities by the fluid pressure applied to the inside.

FIG. 6 is a top view of a micro liquid generating jig according to an embodiment of the present invention, FIG. 7 is a sectional view taken along the line A-A 'of FIG. 6, and FIG. 8 is a sectional view taken along the line B-B' of FIG. 9 is a sectional view taken along the line C-C 'of FIG. 6, and FIG. 10 is a sectional view taken along the line D-D' of FIG.

Referring to FIGS. 1 to 10, the cartridge unit 1100 is provided so as to generate microcapsules therein. The cartridge unit 1100 includes a substrate unit 1110, an oil injecting unit 1120, a sample injecting unit 1130, and a collecting unit 1140 ).

The substrate part 1110 may be provided so that a micro channel (not shown) is formed therein. The micro channel includes the oil injecting part 1120, the sample injecting part 1130 and the collecting part 1140 which are formed one by one in the width direction of the substrate part 1110 as one assembly, And may be provided to connect the flow path.

The oil injecting part 1120 is provided on the upper part of the substrate part 1110 and may inject oil into the micro flow path.

The sample injecting unit 1130 may be provided at one side of the oil injecting unit 1120 to inject the sample into the micro flow path. Here, the sample injected into the micro channel by the sample injecting unit 1130 may include a PCR reagent.

The collecting part 1140 is provided at one side of the sample injecting part 1130 and may be arranged to collect the undiluted solution generated in the micro flow path.

The oil injecting portion 1120, the sample injecting portion 1130 and the collecting portion 1140 may be provided in a plurality of ways and may be provided to form one row on the substrate portion 1110, respectively.

Specifically, a plurality of the oil injecting part 1120, the sample injecting part 1130, and the collecting part 1140 may be provided in the longitudinal direction of the substrate part 1110, respectively. The cartridge unit 1100 thus arranged is arranged such that one of the oil injecting portion 1120, the sample injecting portion 1130 and the collecting portion 1140 is positioned in the width direction of the substrate portion 1110, A plurality of such assemblies may be provided in the longitudinal direction of the substrate unit 1110.

The sample injecting unit 1130 and the oil injecting unit 1120 are provided to discharge the sample and the oil toward the micro flow path by the fluid pressure, respectively, and the sample is separated by the oil to form independent fine droplets Of the total.

Specifically, the sample and the oil are not mixed. Therefore, when the sample injecting portion 1130 and the oil injecting portion 1120 formed in one assembly discharge the sample and the oil into the micro flow path by the fluid pressure, each oil is inserted in the middle of the sample The sample can be separated. The sample thus separated forms an undiluted solution. Then, the generated fine fluid is moved to the collecting part 1140 by the fluid pressure. Here, the fluid pressure includes at least one of a vacuum pressure and a pump pressure.

For this, the micro flow path may be provided to connect the oil injecting portion 1120 and the sample injecting portion 1130 to the collecting portion 1140 in a linear direction. However, the shape of the microchannel is not limited thereto, and the microchannel for transferring the oil injected from the oil injecting portion 1120 may be connected to the microchannel connecting the sample injecting portion 1130 and the collecting portion 1140 And may be provided so as to cross each other.

The flow rate at which the oil injecting section 1120 injects oil into the micro flow path and the flow rate at which the sample injecting section 1130 injects the sample into the micro flow path can be separately controlled. If the flow rate of the oil injector 1120 and the micro flow path are controlled individually, the amount of the sample injected between the oil and the oil can be controlled and the size of the micro fluid can be controlled according to the amount of the sample .

The upper housing unit 1200 may be provided on the upper portion of the cartridge unit 1100 and includes an upper housing part 1210, an oil inlet 1220, a sample inlet 1230, a collecting outlet 1240, 1250).

The upper housing part 1210 may be coupled to the upper part of the base part 1110 and may include a center frame 1211 and a side frame 1212.

The center frame 1211 may be disposed at an upper portion of the substrate 1110 and the oil injection port 1220, the sample inlet 1230 and the collecting outlet 1240 may be formed in the center frame 1211, May be provided.

The side frames 1212 are provided under the center frame 1211 and can be bent and extended toward both sides of the center frame 1211.

In addition, one or more upper housing holes 1213 may be formed in the pair of side frames 1212.

The oil injection port 1220 may be provided in the upper housing part 1210 and may be coupled to the oil injection part 1120. The oil injection port 1220 may be provided in the center frame 1211 and may be provided at a position corresponding to the oil injection portion 1120 provided in the base portion 1110. [ The oil injection port 1220 may be coupled to an upper portion of the oil injection unit 1120.

The sample injection port 1230 may be provided in the upper housing part 1210 and may be coupled to the sample injection part 1130. Specifically, the sample injection port 1230 may be provided in the center frame 1211 and may be provided at a position corresponding to the sample injection unit 1130 provided in the substrate unit 1110. The sample injection port 1230 may be coupled to the upper portion of the sample injection unit 1130.

The collecting outlet 1240 is provided in the upper housing part 1210 and may be provided to be coupled to the collecting part 1140. The collecting outlet 1240 may be provided in the center frame 1211 and may be provided at a position corresponding to the collecting part 1140 provided in the base part 1110. The collecting outlet 1240 may be coupled to an upper portion of the collecting portion 1140.

In addition, an engaging groove 1241 may be formed in the collecting outlet 1240. The engaging groove 1241 is provided so as to engage with a stud portion 1410 of the discharging unit 1400 to be described later. For example, the coupling groove 1241 may be formed in a threaded groove shape, but not limited thereto, and the coupling groove 1241 may be coupled with the stud portion 1410 to maintain airtightness It includes all forms.

The sealing portion 1250 is provided between the collecting outlet 1240 and the discharging portion so that when the generated undiluted solution is transferred from the discharging portion to the collecting outlet 1240, Can be prevented from flowing out through the fine space between them.

The lower housing unit 1300 is provided at a lower portion of the cartridge unit 1100 and may be coupled to the upper housing unit 1200 to fix the substrate unit 1110. [

Specifically, the lower housing unit 1300 includes an inner frame 1310 and an outer frame 1320.

The inner frame 1310 may be provided on the upper portion to hold the substrate portion 1110 thereon. In this case, the length of the inner frame 1310 in the width direction may be the same as the width of the substrate portion 1110.

The outer frame 1320 is provided on both sides of the inner frame 1310 and may be coupled to the side frames 1212 of the upper housing unit 1200.

In addition, the outer frame 1320 may be formed to have a step height higher than that of the inner frame 1310.

One or more lower housing holes 1330 may be formed in the pair of outer frames 1320 and the lower housing holes 1330 may be provided at positions corresponding to the upper housing holes 1213 .

The lower housing unit 1300 may be provided to fix the substrate unit 1110 located inside the outer frame when the outer frame 1320 and the side frame 1212 are coupled to each other.

The distance between the center frame 1211 and the inner frame 1310 when the outer frame 1320 and the side frame 1212 are coupled is larger than the distance between the center of the oil injection port 1220 The sample inlet 1230 and the collecting outlet 1240 may be coupled to the oil injecting portion 1120, the sample injecting portion 1130 and the collecting portion 1140, respectively.

The discharge unit 1400 may be coupled to the upper housing unit 1200 and may be provided to discharge the generated micro fluid such that the stud unit 1410, the tube coupling unit 1420, . ≪ / RTI >

The stud portion 1410 is inserted into the collecting outlet 1240 of the upper housing unit 1200 to be coupled therewith, and a flow path through which the undiluted solution can be discharged may be formed in the stud portion 1410.

Specifically, the stud portion 1410 can be coupled to the engaging groove 1241 formed in the collecting outlet 1240. For example, as shown in the figure, the stud portion 1410 may be threaded on the outer circumferential surface and screwed to the defect groove.

The tube fitting part 1420 may be provided on the upper part of the stud part 1410.

The tube fitting hole 1430 may be provided in the tube fitting portion 1420 so that the tube may be coupled to the tube fitting portion 1420.

The tube fitting hole 1430 may be communicated with a flow path formed inside the stud portion 1410.

The coupling unit 1500 may be inserted and fixed to the inside of the upper housing hole 1213 and the lower housing hole 1330. The coupling unit 1500 may be coupled to the upper housing hole 1213 and the lower housing hole 1330 to couple the upper housing unit 1200 and the lower housing unit 1300. [

As the upper housing unit 1200 and the lower housing unit 1300 are coupled to each other, the cartridge unit 1100 located inside can be fixed.

11 is an exemplary view showing a pump unit of a micro liquid generating jig according to an embodiment of the present invention.

11, the micro liquid generating jig 1000 may further include a vacuum pump unit 1600 and a storage unit 1700.

The storage unit 1700 may be connected to the discharge unit 1400 by the tube and may be arranged to collect and store the undiluted liquid discharged from the discharge unit 1400.

The vacuum pump unit 1600 is connected to the evacuation unit 1400 and the vacuum pump unit 1600 supplies the oil and the sample injected into the oil injection unit 1120 and the sample injection unit 1130 to a vacuum It is possible to inhale and generate micro liquid.

Specifically, the vacuum pump unit 1600 includes a flow rate valve 1610 and a vacuum pump 1620.

The flow rate valve 1610 may be provided between the discharge unit 1400 and the storage unit 1700. The vacuum pump unit 1600 may be disposed between the oil injection unit 1120 and the sample injection unit 1130, And to control the flow rate of the oil and the sample when the vacuum is sucked.

The vacuum pump 1620 vacuum-sucks the oil and the sample contained in the oil injecting unit 1120 and the sample injecting unit 1130 to generate a microcapsule by the above-described method, (1700). At this time, the storage unit 1700 may be provided separately from the vacuum pump 1620, but a storage unit 1700 may be provided in the vacuum pump 1620.

When the vacuum pump 1620 vacuum-sucks the oil and the sample contained in the oil injecting part 1120 and the sample injecting part 1130, the sample is moved to the collecting part 1140 through the micro flow path . The micro flow path for transferring the oil contained in the oil injecting section 1120 to the collecting section 1140 is transferred to the collecting section 1140 by intersecting the micro flow path for transferring the sample to the collecting section 1140 It is possible to generate microcapsules while being injected between the samples.

The micro liquid generating jig 1000 may further include a first pump unit 1800 and a second pump unit 1900.

The first pump unit 1800 is connected to the oil injection unit 1120 and is adapted to apply a pump pressure to the oil injection unit 1120. The second pump unit 1900 is connected to the sample injection unit 1130 and may be provided to apply a pump pressure to the sample injection unit 1130. [

The first pump unit 1800 and the second pump unit 1900 apply pump pressure to the oil and the sample injected into the oil injection unit 1120 and the sample injection unit 1130, An enemy can be generated.

The first pump unit 1800 and the second pump unit 1900 are provided to control the pump pressure applied to the oil injection unit 1120 and the sample injection unit 1130, It is possible to control the size of the undrawn amount.

Specifically, as the pump pressure of the second pump unit 1900 is larger than that of the first pump unit 1800, the flow rate of the sample is increased and the volume of the undiluted solution becomes larger.

The vacuum pump unit 1600 and the first pump unit 1800 and the second pump unit 1900 may be provided at the same time and the first pump unit 1800 and the second pump unit 1900 Only the vacuum pump unit 1600 may be provided without the vacuum pump unit 1600 and only the first pump unit 1800 and the second pump unit 1900 may be provided without the vacuum pump unit 1600.

12 is a photograph of the cartridge unit of the micro liquid generating jig 1000 according to an embodiment of the present invention, and FIG. 13 is a photograph showing the combination of the micro liquid generating jig according to an embodiment of the present invention .

The micro liquid generating jig 1000 provided as described above can be applied to the micro liquid generating apparatus.

In addition, the micro liquid generating jig 1000 provided as described above can uniformly generate micro liquid volume, and it is possible to quickly and easily generate micro liquid liquid by controlling each pump.

Hereinafter, a micro-liquid generating method using the micro liquid generating jig 1000 according to the present invention will be described in detail.

FIG. 14 is a flowchart of a microcavity generation method using a microgass production jig according to an embodiment of the present invention.

Referring to FIG. 14, the microcavity generating method using the microcavity generating jig 1000 can first perform the step S100 of preparing the microcavity generating jig 1000.

A sample is injected into the sample injecting section 1130 of the cartridge unit 1100 and oil is injected into the oil injecting section 1120 in step S100 of preparing the micro liquid generating jig 1000 . Here, the sample injected into the sample injecting unit 1130 may include a PCR reagent.

A fluid pressure is generated in the sample injecting portion 1130 and the oil injecting portion 1120 of the micro liquid generating jig 1000 to form a micro fluid generating jig 1000 A step of generating a tax amount (S200) may be performed.

In the step S200 of generating the undiluted solution by generating the fluid pressure in the sample injecting portion 1130 and the oil injecting portion 1120 of the micro liquid generating jig 1000, the micro liquid generating jig 1000 To generate the fluid pressure in the sample injecting portion 1130 and the oil injecting portion 1120 of the sample analyzer 1110 to discharge the sample and the oil into the micro flow path. The sample discharged into the microchannel in the substrate portion 1110 by the fluid pressure can be separated by the oil to form microcapsules.

Hereinafter, each of the embodiments of generating the undiluted solution by generating fluid pressure in the sample injecting portion 1130 and the oil injecting portion 1120 of the micro liquid generating jig 1000 will be described in detail .

FIG. 15 is a diagram illustrating a micro-liquid generating method using the micro-liquid generating jig according to the first embodiment of the present invention, Fig.

15 and 16, the microcavity generating method using the microcavity generating jig 1000 according to the first embodiment includes a sample injecting unit 1130 of the microcavity generating jig 1000 A sample and oil injected into the sample injecting section 1130 and the oil injecting section 1120 are vacuumed in a step S200 of generating a micro fluid level by generating fluid pressure in the oil injecting section 1120 and the oil injecting section 1120 It is possible to generate an undrawn amount.

Specifically, the microcavity generating method using the microcavity generating jig 1000 according to the first embodiment is a method in which the oil injecting section 1120 and the sample injecting section 1130 And the oil and the sample respectively stored in the oil passage and the oil passage are discharged to the micro flow path.

In particular, according to the first embodiment of the present invention, since the flow of all the fluids is controlled by using the vacuum pump unit 1600, all the connected fluids can be moved at the same flow rate. Thus, when the sample and the oil are moved to the same flow rate, uniform microcapsules can be generated in the microchannels in different assemblies.

That is, according to the first embodiment of the present invention, it is possible to generate a large amount of fine liquid droplets of a uniform size and to control the fluids of various assemblies even if only one pump is controlled.

17 is a diagram illustrating an example of a micro-liquid generating method using a micro-liquid generating jig according to a second embodiment of the present invention.

17, the microcavity generating method using the microcavity generating jig 1000 according to the second embodiment is characterized in that the sample injecting section 1130 of the microcavity generating jig 1000 and the oil A pump pressure is applied to the sample and oil injected into the sample injecting section 1130 and the oil injecting section 1120, respectively, in step S200, in which the fluid pressure is generated in the injecting section 1120, It is possible to generate an undue amount of money.

Specifically, in the microcavity generating method using the micro liquid generating jig 1000 according to the second embodiment, the pump pressure of the first pump unit 1800 and the pump pressure of the second pump unit 1900 are used It is possible to generate an undrawn amount. More specifically, the first pump unit 1800 is connected to the oil injection unit 1120 and is adapted to apply pump pressure to the oil injection unit 1120, and the second pump unit 1900 And may be connected to the sample injecting unit 1130 to apply pump pressure to the sample injecting unit 1130.

The first pump unit 1800 and the second pump unit 1900 apply pump pressure to the oil and the sample injected into the oil injection unit 1120 and the sample injection unit 1130, An enemy can be generated.

The microcavity generating method using the microcavity generating jig 1000 according to the second embodiment is similar to the microcavity generating method using the sample injecting section 1130 and the oil injecting section 1120 of the microcavity generating jig 1000, The pump pressure applied to the sample injecting unit 1130 and the oil injecting unit 1120 is individually controlled in step S200 in which the micro fluid level is generated by generating fluid pressure in the sample injecting unit 1130, .

That is, in step S200 of generating the undiluted solution by generating fluid pressure in the sample injection part 1130 and the oil injection part 1120 of the micro liquid generating jig 1000, By discharging into the micro flow path at different flow rates, the sample can form independent micro droplets in the form of independent micro droplets.

18 is an exemplary view of a microcavity generating method using a microcavity generating jig according to the third embodiment of the present invention.

18, the microcavity generating method using the microcavity generating jig 1000 according to the third embodiment is characterized in that the sample injecting section 1130 of the microcavity generating jig 1000 and the oil In the step S200 of generating the undiluted solution by generating the fluid pressure in the injecting part 1120, the micro-fluid generating method using the micro-fluid generating jig 1000 according to the first embodiment and the micro- It is possible to generate a micro liquid at the same time by using the micro liquid production method using the micro liquid generating jig 1000 according to the present invention.

That is, the microcavity generating method using the microcavity generating jig 1000 according to the third embodiment includes the sample injecting section 1130 and the oil injecting section 1120 of the microcavity generating jig 1000, When the pump pressure is applied to the sample and oil injected into the sample injecting section 1130 and the oil injecting section 1120 in the step of generating microcavities by generating a fluid pressure in the sample injecting section 1130 1130) and the oil injected into the oil injecting portion 1120 and the oil are vacuumed to generate an undiluted solution.

After the fluid pressure is generated in the sample injecting portion 1130 and the oil injecting portion 1120 of the micro liquid generating jig 1000 to generate micro liquid droplets (S200), the generated micro liquid droplets are discharged Step S300 may be performed.

In step S300 of discharging the generated micro liquid, the generated micro liquid can be discharged to the storage unit 1700 through the collecting unit 1140 and the discharging unit 1400. [

The microcavity generating method using the microcavity generating jig 1000 provided as described above can be applied to the microcavity generating apparatus.

FIG. 19 is a photograph showing microcavities generated according to the microcavity generation method using the microcavity production jig according to an embodiment of the present invention. FIG.

Specifically, Fig. 19 (a) shows the size of the micro liquid at 5 μL / min, (b) the micro liquid at 10 μL / min, And the size of the enemy was 40 占 / / min.

As described above, it can be seen that all of the fine liquid droplets generated according to the present invention are formed in a uniform size.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

1000: micro liquid generating jig 1100: cartridge unit
1110: substrate portion 1120: oil injection portion
1130: sample injecting part 1140: collecting part
1200: upper housing unit 1210: upper housing part
1211: center frame 1212: side frame
1213: Upper housing hole 1220: Oil inlet
1230: Sample inlet 1240: Collecting outlet
1241: coupling groove 1250: sealing part
1300: lower housing unit 1310: inner frame
1320: outer frame 1330: lower housing hole
1400: discharging unit 1410:
1420: tube fitting part 1430: tube fitting hole
1500: Coupling unit 1600: Vacuum pump unit
1610: Flow rate valve 1620: Vacuum pump
1700: storage unit 1800: first pump unit
1900: Second pump unit

Claims (14)

A cartridge unit having a substrate portion provided with a micro flow passage formed therein so as to generate micro liquid therein;
An upper housing unit provided on an upper portion of the cartridge unit and having an upper housing portion coupled to an upper portion of the substrate portion;
A lower housing unit provided at a lower portion of the cartridge unit; And
And a discharge unit coupled to the upper housing unit and discharging the generated micro liquid,
Wherein the cartridge unit is provided so as to generate an undiluted solution due to fluid pressure applied thereto,
The upper housing part,
A center frame provided at an upper portion of the substrate portion and having an oil inlet, a sample inlet, and a collecting outlet; And
And a side frame that is provided at a lower portion of the center frame and that is bent and extended toward both sides of the center frame,
The lower housing unit includes:
An inner frame provided on the upper portion to receive the substrate portion; And
And an outer frame provided on both sides of the inner frame and adapted to engage with the side frames of the upper housing unit,
The length of the inner frame in the width direction is the same as the length in the width direction of the substrate portion and the outer frame is formed to have a step height higher than that of the inner frame so that when the outer frame and the side frame are engaged, And the alignment of the oil injecting portion, the sample injecting portion, the collecting portion, the oil injecting port, the sample injection port, and the collecting and discharging port provided in the substrate portion is facilitated. The method according to claim 1,
Wherein the cartridge unit comprises:
An oil injection unit provided at an upper portion of the substrate unit and adapted to inject oil into the micro flow path;
A sample injecting portion provided at one side of the oil injecting portion and adapted to inject a sample into the micro flow path; And
And a collecting portion provided at one side of the sample injecting portion for collecting the undiluted solution generated in the micro flow path. 3. The method of claim 2,
Wherein the oil injecting unit, the sample injecting unit, and the collecting unit are provided in a plurality of units, and each of the oil injecting unit, the sample injecting unit, and the collecting unit is provided to form one row on the substrate. 3. The method of claim 2,
Wherein the sample injecting unit and the oil injecting unit are respectively arranged to discharge the sample and the oil toward the micro flow path by the fluid pressure,
Wherein the sample is separated by the oil to form micro-droplets in the form of independent micro droplets. 3. The method of claim 2,
Wherein the sample injected into the micro channel by the sample injecting unit includes a PCR reagent. 3. The method of claim 2,
The upper housing unit includes:
The oil injection port provided in the upper housing part and adapted to be coupled to the oil injection part;
A sample injection port provided in the upper housing part and adapted to be coupled to the sample injection part; And
And a collecting outlet provided in the upper housing part and adapted to be coupled to the collecting part. delete delete The method according to claim 1,
The discharge unit includes:
A stud portion formed to be inserted and coupled to the inside of the collecting outlet of the upper housing unit and having a flow path through which micro liquid can be discharged; And
And a tube coupling portion extending from an upper portion of the stud portion,
And a tube coupling hole through which the tube is coupled is formed inside the tube coupling portion. 3. The method of claim 2,
And a vacuum pump unit connected to the discharge unit,
Wherein the vacuum pump unit vacuum-sucks the oil and the sample injected into the oil injecting unit and the sample injecting unit to generate microcavity. 3. The method of claim 2,
A first pump unit connected to the oil injecting unit and configured to apply a pump pressure to the oil injecting unit; And
And a second pump unit connected to the sample injecting unit and adapted to apply a pump pressure to the sample injecting unit,
Wherein the first pump unit and the second pump unit apply the pump pressure to the oil and the sample injected into the oil injecting unit and the sample injecting unit to generate the undiluted liquid, . 12. The method of claim 11,
And a vacuum pump unit connected to the discharge unit,
The vacuum pump unit includes:
When the first pump unit and the second pump unit respectively apply pump pressure to the oil and the sample injected into the oil injecting unit and the sample injecting unit, the oil injected into the oil injecting unit and the sample injecting unit, Is vacuum-sucked to generate a micro-fluidic jig. The method according to claim 1,
Further comprising a storage unit for collecting and storing the undiluted liquid discharged from the discharge unit. A microcavity generating apparatus to which the microcavity generating jig according to claim 1 is applied.

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