KR101976280B1 - Composition for female annihilation of pumpkin fruit flies and control method of female pumpkin fruit flies using it - Google Patents

Abstract

The present invention relates to a composition for trapping Paradacus depressus shiraki female, which comprises: protein foods, sugar, fruit juice, terpinyl acetate and insecticides, and to a method for controlling Paradacus depressus shiraki female using the same. The composition for trapping Paradacus depressus shiraki female has high attractivity to Paradacus depressus shiraki females, so that females of the Paradacus depressus shiraki can be effectively controlled.

Description

TECHNICAL FIELD The present invention relates to a composition for females and to a method for controlling females such as pumpkin fruit flies and control methods,

The present invention relates to a composition for harvesting females and to a method for preventing and controlling females of squash fruit flies using the same.

Fruit parasites, which are of great concern to crops, belong to the fruit fly (Tephritoidea), which is a large taxon with 9 families and about 7,300 species of bloody blooms (Korneyev, 1999).

It is known that Tephritidae, which has the largest bloom bankruptcy and economic damage to crops, contains more than 4,400 species (White and Elson-Harris, 1992; Norrbom et al. 1999).

The species belonging to the domestic fruit fly family is known as 90 species throughout the two Koreas (Han and Kwon, 2010; Han et al., 2014). They all belong to four subspecies, including four species in the Dacinae subspecies, four species in the Phytalmiinae subspecies, 39 species in the Tephritinae subspecies, and 43 species in the Trypetinae subspecies. The bactrocera depressa and B. scutellata , known as pests, belong to the Dacinae subfamily.

Pumpkin fruit flies were collected and recorded for the first time in 1974 in Baekyangsan, Gwangyang, Jeollanam-do (Kim and Kim, 1974). , 1994). The host plants of pumpkin fruit flies found in Korea are pumpkin (pumpkin, pumpkin), gourd, watermelon and melon (Han et al., 1994), wild Trichosanthes kirilowii ) (Kim et al., 2011). In the 1991-1992 national survey, the average damage rate was reported to be 43.5%, centered on the 300 to 399 meters above sea level (Han et al., 1994).

Pumpkin fruit flies are wintered in pupae, and occur once a year in Korea. The foliage occurs in late May to early June, and sporadic spawning is usually observed in mid-July (Kang et al., 2008). Pumpkin fruit flies are not yet known to be specific attractants, but are attracted to protein feed, such as brewer hydrolysate and Torula yeast (Kim et al . 2010), and when the roosting host was adjacent, the attractiveness was found to increase (Kim et al., 2012).

Amaranth Fruit Flies were not widely treated as agricultural pests because they made the aquatic plant buds of acute plants a food source (Shiraki, 1968). In Korea, Jung (1994) and Kim and Chang (1982) reported on the basic data of amber fruit, Kim et al . (2010) has been reevaluated as a new insect of pumpkin as it reported that 30.7% of the pumpkin and 53.8% of the males were caused by the pumpkin fruit flies.

In Korea, acarid fruit flies occur at the middle of July to early August and early September (Kim et al., 2010). Amber fruit fly has been reported to be attracted to Cuelure (Kim et al., 2012).

Pumpkin Fruit The larvae of the parasite inhabit the inside of the pumpkin and the fruit, and it is difficult to control with the general chemical agent because it is difficult to directly expose the drug because it is harmful. Therefore, control of these fruit flies is targeted to adult exposed to the outside.

Adult fruit flies can be largely classified into two types: genetic techniques using attractants and genetic control techniques using infertility (Vargas et al., 2015). A male annihilation technique (MAT) was developed to add the insecticide to such an attractant by using the effect that the raspberry ketone (RK) or the currant was specifically attracted to the amber fruit fly. (Kim et al., 2017). In the case of females, a female annihilation technique (FAT) has been developed in which females are required to consume protein for their reproduction, and protein feed is used as an attractant and the insecticide is added to the females to induce suicide (Vargas et al. , 2015).

Korean Patent Publication No. 10-2012-0050592 Korean Patent Registration No. 10-0971314

 Kim, Kyu - Soon, Min - Hyun Kim, Kwon Kyung Kwon, and Yong Kyun Kim. 2017. Development of Trap-type male eradication technology based on Fruit fly Fruits. Han Gong Geon. 56, 51-60.  Han, M.J., Lee, S.H., Ahn, S.B., Choi, J.Y., Choi, K.M., 1994. Distribution, Damage and Host Plants of Pumpkin Fruit Fly, Paradacus depressa (Shiraki). RDA J. Agri. Sci. 36, 346-350.  Han, H.Y., Kwon, Y.J., 2010. A list of North Korean Tephritoid species (Diptera: Tephritoidea) deposited in the Hungarian natural history museum. Kor. J. Syst. Zool. 26, 251-260.  Han, H.Y., Suk, S.W., Lee, Y.B., Lee, H.S. 2014. National List of Species of Korea "Insect" (Diptera II). National Institute of Biological Resources. Incheon. 1-268 pp.  (Diptera: Tephritidae), an adult emergence model of pumpkin fruit fly, Bactrocera depressa (Diptera: Tephritidae). Kor. J. Agric. For. Meteor. 47, 407-411.  Kim, J. I., Chang, K.S., 1982. On the summer seasonal insects from the group of Soan island, Wando-kun. Rep. Survey Nat. Environ. Korea. 2, 161-184.  Kim, C. W., Kim, J. L., 1974. Insect fauna of national park, Mt. Naejang-san in summer season. Rep. Kor. Cons. Nat. 8, 85-126.  Kim, D.S., Jang, Y.S., Choi, K.S., Kang, T.J., Jeon, H.Y., 2012. Olfactory responses of Bactrocera depressa (Diptera: Tephritidae) in the field and laboratory. J. Subtrop. Agri. Biotechnol. 28, 33-44.  Kim, Y.P., Jeon, S.W., Lee, S.G., Choi, N.J., Hwang, C.H., 2010. Seasonal occurrence and damage of Bactrocera scutellata (Diptera: Tephritidae) in Jeonbuk province. Kor. J. Appl. Entomol. 49, 299-304.  Kim, D.S., Kang, T.J., Jeon, H.Y., 2011. A preliminary study for the visual response of Bactrocera depressa (Tephritidae: Diptera) in the laboratory environment. J. Subtrop. Agri. Biotechnol. 27, 39-44.  Korneyev, V. A., 1999. Phylogenetic relationships among the families of the superfamily Tephritoidea, in: Aluja, M., Norrbom, A. L. (Eds.), Fruit flies (Tephritidae): phylogeny and evolution of behavior. CRC Press, pp. 3-22.  Norrbom, A. L., Carroll, L. E., Thompson, F. C., White, I. M., Freidberg, A., 1999. Systematic database of names, in: Thompson, F.C. (Ed.), Fruit fly expert system and systematic information database, Diptera Data Dissemination Disk 1 & Myia. pp. 65251.  Shiraki, T., 1968. Fruit flies of the Ryukyu islands. United States National Museum Bulletin 263 pp.  Vargas, R. I., Pinero, J. C., Leblanc L., 2015. An overview of pest species of Bactrocera fruit flies (Diptera: Tephritidae) and their integration with biopesticides. Insects 6, 297-318.  White, I. M., Elson-Harris, M. M., 1992. Fruit Flies of Economic Significance: Their Identification and Bionomics. CAB International / ACIAR.  White, I.M., Elson-Harris, M.M., 1994. Fruit flies of economic significance: their identification and bionomics. 2nd Edtion, CAB, Wallingford, 601 pp.

The present invention relates to a composition for damaging females of amber fruit flies including amber fruit flies and amber fruit flies, and a method for controlling the amber flies by using the composition.

In order to accomplish the above object, the present invention provides a method for producing a protein-containing food, which comprises protein feed, sugar, fruit juice, terpinyl acetate and insecticide, wherein the protein feed and sugar are mixed at a weight ratio of 1: 3 to 3: Wherein the mixture of 75 to 95 ml of fruit juice, 1 to 4 ml of terpineacetate and 1 to 4 ml of insecticide is mixed on the basis of 100 g of the mixture.

The composition may further comprise sodium azide.

In the above composition, sodium azide is preferably mixed with 0.6 to 1.4 g based on 100 g of a mixture of protein and sugar.

In the above composition, it is preferable that the protein-eating protein hydrolyzate, at least one selected from raw yeast and yeast extract.

The present invention also provides a method for preventing and controlling the females of Pumpkin Fillipulus by using a composition for feminine fruit fly parasitization.

In the method, the pumpkin fruit flies include pumpkin fruit flies and amber fruit flies.

The present invention relates to a composition for feminization of females which increases the attractiveness to females of the pumpkin fruit parasite by adding fruit juice and terpineacetate based on the protein feed and sugar to be fed to the amber fruit family, It has a high attraction for both pumpkin fruit flies and amber fruit flies. It is confirmed that the ratio of females to both males and females of the pumpkin fruit flies is up to 90%, which is different from that of the conventional males, so that the females are killed and controlled.

FIG. 1 shows the results of confirming the attractiveness of pumpkin fruit flies by dilution concentration of commercial fruit flour spraying agent (GF120).
FIG. 2 shows a comparison of the attractiveness of pumpkin fruit flies according to the trap position of commercial fruit flour spraying agent (GF120).
FIG. 3 is a graph showing the comparison of the entrainment capacity of pumpkin fruit flies of a commercial fruit fly spraying agent (GF120) of the females in Example 1.
Fig. 4 shows the results of comparing the attracting powers of pumpkin fruit flies of the composition for flesh transfer of females of Examples 1 and 2. Fig.
Figs. 5 to 7 show results of comparisons of attracting powers of pumpkin fruit flies of the compositions for fecal loss of females of Examples 2 to 4. Fig.
FIGS. 8 to 10 are the results of comparing the attracting power of terpineacetate (TA) to amber fruit flies.

The present invention relates to a composition for feminization of amber fruit, including protein food, sugar, fruit juice, terpinyl acetate and insecticides.

In this composition, protein food, sugar, fruit juice, and terpinyl acetate are all ingredients that attract pumpkin fruit flies, especially females.

Pumpkin fruit flies include pumpkin fruit flies and amber fruit flies.

Pumpkin fruit parasitic females are able to spawn only by ingesting protein hydrolyzate, so protein feed is used to attract females. As a protein feed, a protein hydrolyzate, a raw yeast extract, and an yeast extract, which are commonly used, are used.

Sugar is also used as food for pumpkin fruit flies, so it has an attracting effect.

Preferably, the protein feed and the sugar are mixed in a weight ratio of 1: 3 to 3: 1.

Fruit juice may be prepared from various fruits or used on the market. Examples of fruit juices are ordinary fruit juices such as oranges, grapes, and mangoes. The fruit juice is preferably used in an amount of 75 to 95 ml based on 100 g of a mixture of protein and sugar, more preferably 80 to 90 ml.

It is preferable to use 1 to 4 ml of terpinyl acetate based on 100 g of a mixture of protein and sugar, more preferably 2 to 3 ml.

By adding the fruit juice and terpineacetate to the composition, the attractiveness to amber fruit flies is markedly increased.

Insecticides are not pumpkin fruit fly acids can be used a variety of pesticides with insecticidal effect on, it is used in this embodiment eungchil ^® environmentally friendly insecticides, spinosad and the like, but including the limited. The insecticide is preferably used in an amount of 1 to 4 ml based on 100 g of a mixture of protein and sugar, more preferably 2 to 3 ml.

The composition for sowing the females may further comprise sodium azide. Sodium azide acts as a preservative and prevents outdoors the growth of bacteria and fungi by the protein diet and sugar contained in the composition for the slaughter of females. The sodium azide is preferably used in an amount of 0.6 to 1.4 g based on 100 g of a mixture of protein and sugar, more preferably 0.8 to 1.2 g.

The above-described composition for feminine sowing may be diluted by mixing distilled water. The distilled water can be appropriately added according to the desired dilution ratio.

The composition of the present invention of the present invention can be used for the control of females such as amber fruit flies. As a typical method, there is a method of treating the above-mentioned composition in a trap and installing the trap in an area where control is required, but the present invention is not limited thereto.

Hereinafter, the present invention will be described in more detail with reference to examples. The following examples illustrate the invention and are not intended to limit the scope of the invention.

< Example  1>

250 g of yeast extract (BioShop, Burlington, Canada), 750 g of sugar (CJ, Incheon, Korea) and 1 g of spinosad (Boomerang, Dongbang Agro, Seoul, Korea) FAT101).

< Example  2>

Yeast extract (MB cell, Seoul, Korea) 25g, Sugar (CJ CJ, Incheon, South Korea) 70g, fruit juice (cold ^®, Lotte Chilsung, Seoul, Korea) 80㎖, pesticides (eungchil the ^®, considering bio, (Sigma, Seoul, Korea), 2 ml of distilled water (Sigma, Seoul, Korea) and 2 ml of distilled water were mixed to prepare a composition for fecundation of pumpkin fruit parley females (FAT201) .

< Example  3>

Yeast extract (MB cell, Seoul, Korea) 50g, Sugar (CJ CJ, Incheon, South Korea) 45g, fruit juice (cold ^®, Lotte Chilsung, Seoul, Korea) 80㎖, pesticides (eungchil the ^®, considering bio, (Sigma, Seoul, Korea), 2 ml of distilled water (Sigma, Seoul, Korea) and 40 ml of distilled water were mixed to prepare a composition for fecundation of amber fruit pods (FAT202) .

< Example  4>

Yeast extract (MB cell, Seoul, Korea) 75g, Sugar (CJ CJ, Incheon, South Korea) 20g, fruit juice (cold ^®, Lotte Chilsung, Seoul, Korea) 80㎖, pesticides (eungchil the ^®, considering bio, (Sigma, Seoul, Korea), 2 ml of distilled water (Sigma, Seoul, Korea) and 40 ml of distilled water were mixed to prepare a composition for fecundation of amber fruit pods (FAT203) .

[ Experimental Example ]

Analysis packaging test

Test place and test insect

Analytical pavement test sites were Gyeongli (Northeast 36.53 East 128.77) in Andong, Gyeongbuk Province, and Amber Field (North Korea 33.44 East 126.57) belonging to Arudong in Jeju City and surrounding areas were Trichosanthes There is kirilowii . japonica Kitam) was born. Analysis All of the insects used in the pavement test consisted of outdoor packs.

sample

As a test sample, the composition for flesh transfer of females prepared in Examples 1 to 4 was used. For comparison, GF120, a commercial fruit fly spraying agent, was used.

Trap installation and packaging test

The compositions for female sowing were treated with McPhil trap (McP: Pherobank, Wijk bij Duurstede, Netherland). Each of the traps was treated with 500 ml of the herbicidal composition. However, since the herbicidal composition of Example 1 was solid, about 100 g was administered to each trap. The distance between traps was kept at least 50m and tested repeatedly 3 times in each package.

The tests were carried out from June 7 to July 21, 2017 in Jeju Island packaging, and from August 11 to September 21 in Andong packaging.

The results are expressed as the average trapped number trapped in each trap for a given period of time.

Statistical analysis

Percentage data were used for analysis of variance through arcsin numerical transformations. For the analysis of variance, PROC GLM of SAS program (SAS Inc., 1989) was used.

< Experimental Example  1>

Commercial Fruit of Paris Fertilizer Pumpkin Fruit Parsley  Incentive test

GF120, which is used as a fruit flour spraying agent in foreign countries, is a medicine that gives spinosad insecticide to the effect of ingestion derived from food materials to produce a hair loss effect. This drug was applied to pumpkin fruit flies inhabited in Korea.

1. Stimulus test by dilution concentration

The commercial fruit flies spraying agent (GF120) was diluted to 5 times, 20 times, 50 times and 500 times, and the results are shown in Fig.

As shown in Fig. 1, the commercial fruit flour spraying agent (GF120) showed a difference in attractiveness depending on the concentration of the drug ( F = 3.45; df = 3,8; P = 0.0717).

Interestingly, commercial fruit fly spraying agent (GF120) showed almost no attracting effect in the range of dilution concentration of 5 ~ 50 times. However, as the dilution concentration was increased 500 times, the incentive effect was significantly increased. However, the average number of attracted pumpkin fruit flies was about 5.7 for about 2 weeks (June 7 ~ June 21), indicating very low attractiveness.

2. Examination of attraction by trap position

The 500-fold dilution of commercial fruit flour spraying agent (GF120) was divided into sun and shade for 10 days (from June 21 to June 30). The sunshine was installed in the roadside exposed area, and the shade was installed under the roadside tree in the same place. The results are shown in Fig.

As shown in Fig. 2, there was a significant difference in attractiveness between the sunny and shaded areas ( F = 27.00; df = 1.4, P = 0.0065). At the same time, the catch density was not observed at all in the sunshine, but only at the trap installed under the shade of the tree.

As such, the commercial fruit fly spraying agent (GF120) showed an attractive attractiveness in a trap installed in a shade at a 500-fold dilution.

< Experimental Example  2>

Commercial fruit fly sprayer Example  Comparison of attractiveness of composition of 1

The attractiveness of commercial fruit flies spraying agent (GF120) and the composition for fecal slaughtering (FAT101) of Example 1 were compared.

A 500-fold dilution was used for commercial fruit flies (GF120), and a 50-fold dilution ( F = 9.14; df = 1,4; P = 0.0390) The results are shown in Fig.

As shown in the results of FIG. 3, the composition for fecal slaughter (FAT101) of Example 1 was significantly more effective than the commercial fruit fly spraying agent (GF120) on the amber fruit fly.

< Experimental Example  3>

Terpinyl  Acetate and Fruit juice Whether to add  Comparing attraction

In order to compare the attraction of pumpkin fruit flies according to the addition of terpinyi acetate and fruit juice, the following experiment was carried out.

As a sample, the composition for fecalization of females (FAT101) of Example 1 and the composition for flesh transfer of females (FAT201) of Example 2 were used. A 50-fold dilution was used for the composition for fecundity (FAT101) in Example 1, and 10-fold, 20-fold and 100-fold dilutions for the composition for fecal insemination (FAT201) in Example 2 were used. The results are shown in Fig.

The attractiveness of the composition for fecal insemination (FAT201) of Example 2 was different depending on the concentration and the attractivity was significantly higher in the 100-fold dilution ( F = 20.23; df = 3.5; P = 0.0032).

< Experimental Example  4>

Protein food and  Comparison of incentives according to sugar content

The differences in the attractiveness of pumpkin fruit flies according to the contents of protein and sugar were investigated as follows.

(FAT201) of Example 2, a female herbicidal composition (FAT202) of Example 3 and a female herbicidal composition (FAT203) of Example 4 were used as samples. From Example 2 to Example 4, the ratio of protein to sugar increases.

These three types of compositions for the squeezing were diluted 50 times to compare the attracting power against pumpkin fruit flies. The results are shown in Figs. 5 to 7.

As shown in the results of FIG. 5 showing the total number of attracted pumpkin fruit flies, there was no difference in the total catch numbers of three kinds of females ( F = 0.10; df = 2, 9; P = 0.9048).

As shown in FIG. 6, in which the number of females in the trapped pumpkin fruit flies was confirmed, all three kinds of females were found to induce more than 60% of the females, and there was no difference between the compositions for females ( F = 0.19; df = 2.9; P = 0.8287).

As can be seen from the results of FIG. 7 which confirmed the attractiveness of pumpkin fruit flies (Bd) and amber fruit flies (Bc), three kinds of compositions for females were attracted to both species. Among them, (Bd) included up to an average of 14 to 44% ( F = 2.14; df = 2, 9; P = 0.1739).

< Experimental Example  5>

Terpinyl  Confirmed the effect of increase in attractiveness by acetate

Whether the increase in attracting power to the amber fruit flies of the composition of the present invention was due to terpineacetate was confirmed as follows.

(FAT201) obtained in Example 2 and a sample (FAT201-TA) in which terpinyl acetate (TA) was removed from the composition for transferring female feces in Example 2 was examined. The results are shown in Figures 8-10.

In Figure 8, which shows the total attracted pumpkin fruit flies populations, it was found that the attractiveness was relatively pronounced when there was no terpinyl acetate (TA) ( F = 5.11; df = 1,6; P = 0.0456).

On the other hand, as shown in FIG. 9, the attractiveness to females was constantly maintained at about 70% ( F = 0.01; df = 1,6; P = 0.9260) regardless of the presence or absence of terpinyl acetate (TA).

As can be seen from the results of FIG. 10, there was no difference in the effect of attracting the two kinds of pumpkin fruit flies ( F = 2.30; df = 1,6; P = 0.1804).

Claims (6)

Protein hydrolyzate, at least one protein feed selected from raw yeast and yeast extract; Sugar; Fruit juice; Terpinyl acetate; And an insecticide,
Protein food and sugar are mixed in a weight ratio of 1: 3 to 3: 1,
Wherein the mixture is prepared by mixing 75 to 95 ml of fruit juice, 1 to 4 ml of terpinyl acetate and 1 to 4 ml of insecticide based on 100 g of a mixture of protein and sugar. The method according to claim 1,
Wherein the composition further comprises sodium azide. 3. The method of claim 2,
Wherein said sodium azide is admixed with 0.6 to 1.4 g based on 100 g of a mixture of protein feed and sugar. delete A method for preventing and controlling the females of an amber fruit family using a composition for harvesting amber fruit parasol females according to any one of claims 1 to 3. 6. The method of claim 5,
Wherein the pumpkin fruit flies include pumpkin fruit flies and amber fruit flies.

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