KR101971634B1 - Multi-channel microchannel measuring apparatus which can easily replace sensor chip unit and method for measuring the same - Google Patents

Multi-channel microchannel measuring apparatus which can easily replace sensor chip unit and method for measuring the same Download PDF

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Publication number
KR101971634B1
KR101971634B1 KR1020170131673A KR20170131673A KR101971634B1 KR 101971634 B1 KR101971634 B1 KR 101971634B1 KR 1020170131673 A KR1020170131673 A KR 1020170131673A KR 20170131673 A KR20170131673 A KR 20170131673A KR 101971634 B1 KR101971634 B1 KR 101971634B1
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KR
South Korea
Prior art keywords
unit
prism
fixing
sensor chip
film
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KR1020170131673A
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Korean (ko)
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KR20190040839A (en
Inventor
이문근
정순우
이경균
배남호
이태재
이석재
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한국과학기술원
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Priority to KR1020170131673A priority Critical patent/KR101971634B1/en
Priority to PCT/KR2017/011273 priority patent/WO2019074140A1/en
Publication of KR20190040839A publication Critical patent/KR20190040839A/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/21—Polarisation-affecting properties
    • G01N21/211—Ellipsometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/21—Polarisation-affecting properties
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/21—Polarisation-affecting properties
    • G01N21/211—Ellipsometry
    • G01N2021/212—Arrangement with total internal reflection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/41—Refractivity; Phase-affecting properties, e.g. optical path length
    • G01N21/4133—Refractometers, e.g. differential
    • G01N2021/414—Correcting temperature effect in refractometers

Abstract

More particularly, the present invention relates to a multi-channel microchannel measuring device and a method of measuring the same, and more particularly, to a multi-channel microchannel measuring device and a measuring method thereof that are excellent in airtightness of a microfluidic channel, Channel microchannel measuring apparatus and a measuring method therefor. The structure of the present invention is a film-type unit having a microfluidic channel through which a sample containing a target material can pass; A prism unit provided on the film type unit and made of an optical prism; A sensor chip unit provided at a lower portion of the film-type unit and having a surface on which a reaction material reacting with the target material is fixed; A prism fixing unit provided so as to closely contact the film-type unit and the prism unit; And a sensor chip fixing unit provided so as to closely contact the film-type unit and the sensor chip unit, wherein the prism fixing unit and the sensor chip fixing unit are arranged such that the film-type unit, the prism unit, Channel microchannel measurement device that is easy to replace the sensor chip unit.

Description

TECHNICAL FIELD [0001] The present invention relates to a multi-channel microchannel measuring apparatus and a measuring method thereof,

More particularly, the present invention relates to a multi-channel microchannel measuring device and a method of measuring the same, and more particularly, to a multi-channel microchannel measuring device and a measuring method thereof that are excellent in airtightness of a microfluidic channel, Channel microchannel measuring apparatus and a measuring method therefor.

Reflectometry and Ellipsometry are optical techniques that detect the thickness or optical properties of a sample by measuring the change in reflectance or polarization state of the reflected light reflected from the surface of the sample and analyzing the measured value.

Reflectometer and Ellipsometer are used as measuring instruments. They are used to evaluate various nano-scale film thicknesses and physical properties in the nanofiltration process of the semiconductor industry. In addition, efforts to extend the application range to bio-industry and apply it to the interface analysis of biomaterials such as protein, DNA, virus, and new drug substance are continuing.

Particularly, in the field of biosensors, an assembly for forming a fine flow path between a prism and a silicon sensor chip has been developed.

However, in order to ensure the airtightness of the microfluidic channel, the conventional assembly is sealed with an adhesive between the prism and the silicon sensor chip to integrate them. Therefore, in the conventional assembly, it is difficult to separate the silicon chip and the prism, and after the experiment, the silicon sensor chip and the prism have all to be replaced.

Therefore, it is necessary to provide an economical multichannel microchannel measuring device because it is excellent in airtightness, but the silicon sensor chip and the prism can be easily separated to select and replace only the silicon sensor chip.

Korean Patent No. 10-1105328

An object of the present invention to solve the above problems is to provide a multichannel microchannel measuring device which is excellent in airtightness of a microfluidic channel and in which a sensor chip unit can be easily separated and a user can easily replace a sensor chip unit, Method.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the precise form disclosed. There will be.

According to an aspect of the present invention, there is provided a film-type unit including a microfluidic channel through which a sample containing a target material can pass; A prism unit provided on the film type unit and made of an optical prism; A sensor chip unit provided at a lower portion of the film-type unit and having a surface on which a reaction material reacting with the target material is fixed; A prism fixing unit provided so as to closely contact the film-type unit and the prism unit; And a sensor chip fixing unit provided so as to closely contact the film-type unit and the sensor chip unit, wherein the prism fixing unit and the sensor chip fixing unit are arranged such that the film-type unit, the prism unit, Channel microchannel measurement device that is easy to replace the sensor chip unit.

In an embodiment of the present invention, the film-type unit comprises: a microfluidic film made of a material having elasticity; And a microfluidic channel extending in the longitudinal direction of the microfluidic film, the microfluidic channel being provided in at least one of the microfluidic channels.

In an exemplary embodiment of the present invention, the prism unit may include: a prism body forming a body and formed of an optical prism; An incident surface formed on one side surface of the prism body and having an inclined surface; And a reflective surface formed on the other surface of the prism body and provided with an inclined surface, wherein incident light enters the incident surface and reflected light is emitted from the reflective surface.

In an embodiment of the present invention, the incident surface and the reflection surface are inclined surfaces having an inclination angle of 25 degrees to 35 degrees.

In an exemplary embodiment of the present invention, the prism unit may include a sample injection port formed on one side surface of the prism body, the sample port formed below the incident surface; And a sample outlet formed on the other side of the prism body and formed on the lower side of the reflecting surface, wherein the sample inlet and the sample outlet are formed to extend toward the inside of the prism body to be connected to the microfluidic channel . ≪ / RTI >

In an embodiment of the present invention, the prism fixing unit may include: a first fixing part on which the prism unit is seated; A second fixing part which is located opposite to the first fixing part and on which the prism unit is mounted; And an upper fixing unit coupled to an upper portion of the first fixing unit and the second fixing unit to fix the prism unit.

In an exemplary embodiment of the present invention, the first fixing unit may include: a first fixing unit adapted to be in close contact with a side surface of the prism unit; A pair of first elongated bodies extending from the first fixing body so as to surround both side surfaces of one side of the prism unit; And a first mounting member formed stepwise in the inner direction of the first fixing member so that one side of the prism unit is seated.

The first fixing unit may further include a first light blocking inner surface formed on an upper surface of the first fixing member and having a downward inclined surface toward an incident surface of the prism unit .

According to an embodiment of the present invention, the first fixing unit may include: a first conveying passage formed inside the first fixing unit and connected to a sample injection port of the prism unit; And a first tube connection port inserted into the first elongated body and having one side connected to the tube and the other side connected to the first transfer channel.

According to an embodiment of the present invention, the second fixing portion may include: a second fixing body adapted to be in close contact with the other side surface of the prism unit; A pair of second elongated bodies extending from the second fixing body so as to surround opposite side surfaces of the other side of the prism unit; And a second mounting member formed in a stepped manner in an inner direction of the second fixing member so that the other side of the prism unit is seated.

In the embodiment of the present invention, the second fixing portion may further include a second light-blocking inner surface formed on the upper surface of the second fixing body, the second light-blocking inner surface being provided with a downward inclined surface toward the reflection surface of the prism unit .

According to an embodiment of the present invention, the second fixing portion may include a second conveying path formed inside the second fixing member and connected to a sample outlet of the prism unit; And a second tube connection port inserted into the second elongated body and having one side connected to the tube and the other side connected to the second transfer channel.

According to an embodiment of the present invention, the upper fixing portion may include: a pair of first upper fixing bodies connected to the first fixing portion and the second fixing portion; And a second upper fixing body extending to connect a pair of the first upper fixing bodies, wherein when the first upper fixing body is coupled to the first fixing portion and the second fixing portion, And the upper fixture may be configured to press the prism unit downward.

In an embodiment of the present invention, the sensor chip fixing unit may include: a sensor chip fixed body inserted into a lower portion of the prism fixing unit; And a sensor chip protrusion formed on the sensor chip fixed body and on which the film-type unit and the sensor chip unit are mounted, and the prism fixing unit And the second electrode is coupled to the second electrode.

In the embodiment of the present invention, the sensor chip protruding body, the film-like unit and the sensor chip unit are located inside the first and second seating bodies of the prism fixing unit, The thickness of the film-type unit and the sensor chip unit may be the same as the thickness of the first and second seating members.

In an embodiment of the present invention, it may further comprise a polarization detection unit configured to irradiate light toward the prism unit and detect a change in polarization of the reflected light.

In the embodiment of the present invention, the polarization detection unit may include: a light source unit that emits light toward an incident surface of the prism unit; A polarizer for polarizing the light emitted from the light source unit; A lens unit receiving light emitted from the light source unit and providing parallel light to the polarizer; An analyzer for polarizing the reflected light that has passed through the reflection surface of the prism unit; And a photodetector for detecting the polarization change of the polarized reflected light.

According to an aspect of the present invention, there is provided a biosensor device using a multi-channel microchannel measurement device that is easy to replace a sensor chip unit.

According to another aspect of the present invention, there is provided a method for measuring a multi-channel microchannel measuring apparatus, the method comprising the steps of: a) injecting a sample solution into the film- Reacting the target material with a reactive material immobilized on the sensor chip unit; b) illuminating the prism unit with light to detect a polarization change; And c) injecting air into the film-type unit to discharge the sample solution. The method for measuring a multi-channel microchannel measuring apparatus according to claim 1,

In an embodiment of the present invention, the method may further include d) after the step c), removing the sensor chip fixing unit to replace the sensor chip unit.

According to the present invention, the sensor chip fixing unit and the prism fixing unit are provided so as to press the film-type unit, the prism unit, and the sensor chip unit in the up-and-down direction.

Further, according to the present invention, when the tightness of the sensor chip fixing unit is released, the sensor chip unit can be immediately separated, and the sensor chip unit can be easily replaced after the experiment.

Conventionally, after the experiment, the sensor chip unit and the prism unit must be exchanged at the same time. However, according to the present invention, since the sensor chip unit can be replaced only after the experiment, it is economical.

It should be understood that the effects of the present invention are not limited to the above effects and include all effects that can be deduced from the detailed description of the present invention or the configuration of the invention described in the claims.

1 is a perspective view of a multi-channel microchannel measuring apparatus according to an embodiment of the present invention.
FIG. 2 is an exploded perspective view of a multi-channel microchannel measuring apparatus according to an embodiment of the present invention. FIG.
3 is an exploded perspective view of a film-type unit, a prism unit, and a sensor chip unit according to an embodiment of the present invention.
FIG. 4 is a perspective view illustrating a combination of a film-type unit, a prism unit, and a sensor chip unit according to an embodiment of the present invention.
5 is a front view of a film-type unit, a prism unit, and a sensor chip unit according to an embodiment of the present invention.
FIG. 6 is a front view showing a path of light of a multi-channel microchannel measuring apparatus that can be easily replaced with a sensor chip unit according to an embodiment of the present invention.
FIG. 7 is a flowchart illustrating a method of measuring a multi-channel microchannel measuring apparatus according to an embodiment of the present invention. FIG.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described with reference to the accompanying drawings. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. In order to clearly illustrate the present invention, parts not related to the description are omitted, and similar parts are denoted by like reference characters throughout the specification.

Throughout the specification, when a part is referred to as being "connected" (connected, connected, coupled) with another part, it is not only the case where it is "directly connected" "Is included. Also, when an element is referred to as " comprising ", it means that it can include other elements, not excluding other elements unless specifically stated otherwise.

The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The singular expressions include plural expressions unless the context clearly dictates otherwise. In this specification, the terms "comprises" or "having" and the like refer to the presence of stated features, integers, steps, operations, elements, components, or combinations thereof, But do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components, or combinations thereof.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings.

FIG. 1 is a perspective view of a multi-channel microchannel measuring apparatus which can easily replace a sensor chip unit according to an embodiment of the present invention. FIG. 2 is a perspective view of a multi-channel microchannel measuring apparatus according to an embodiment of the present invention, Fig. 3 is an exploded perspective view of a microchannel measurement device.

FIG. 3 is an exploded perspective view of a film-type unit, a prism unit, and a sensor chip unit according to an embodiment of the present invention. FIG. And FIG. 5 is a front view of a film-type unit, a prism unit, and a sensor chip unit according to an embodiment of the present invention.

As shown in FIGS. 1 to 5, a multi-channel microchannel measuring apparatus 1000 that can easily replace a sensor chip unit includes a film-type unit 1100, a prism unit 1200, a sensor chip unit 1300, Unit 1400, a sensor chip fixing unit 1500, and a polarization detection unit 1600. [

In the multi-channel microchannel measuring apparatus 1000 in which the sensor chip unit can be easily replaced, the prism fixing unit 1400 and the sensor chip fixing unit 1500 are mounted on the film-type unit 1100, the prism unit 1200 and the sensor chip unit 1300 are pressed and fixed so as to be in close contact with each other.

The film-type unit 1100 includes a microfluidic film 1110 and a microfluidic channel 1120, and a sample containing the target material may be passed through the film-like unit 1100.

The microfluidic film 1110 may be made of a material having elasticity. More specifically, the microfluidic film 1110 may be formed of a material having elasticity such as a soft polymer or silicon.

The thickness of the microfluidic film 1110 may be less than 1 mm.

The microfluidic channel 1120 may extend in the longitudinal direction of the microfluidic film 1110 to allow a sample containing the target material to pass therethrough, and may be provided in one or more of the microfluidic channels 1120.

The microfluidic channel 1120 may provide a space through which the injected sample solution can react with a reactive material fixed on the surface of the sensor chip unit 1300.

The prism unit 1200 may be provided on the upper portion of the film-type unit 1100 and may be formed of an optical prism. The prism unit 1200 includes a prism body 1210, an incident surface 1220, a reflecting surface 1230, a sample inlet 1240, and a sample outlet 1250.

The prism body 1210 forms the body of the prism unit 1200 and may be formed of an optical prism. That is, the prism body 1210 may be formed of a material having a high optical characteristic such as glass.

The incident surface 1220 may be formed on one side of the prism body 1210 and may have an inclined surface.

The reflecting surface 1230 may be formed on the other surface of the prism body 1210 and may have an inclined surface.

The incident surface 1220 may be provided with incident light, and the reflection surface 1230 may be provided with reflected light.

The incident surface 1220 and the reflection surface 1230 may be inclined surfaces having an inclination angle of 25 degrees to 35 degrees.

The sample inlet 1240 is formed on one side of the prism body 1210 and may be formed on the lower side of the incident surface 1220.

The sample outlet 1250 is formed on the other side of the prism body 1210 and may be formed on the lower side of the reflecting surface 1230.

The sample inlet 1240 and the sample outlet 1250 may extend toward the inside of the prism body 1210 to be connected to the microfluidic channel 1120.

The sample inlet 1240 and the sample outlet 1250 may be the same as the number of the microfluidic channels 1120, respectively.

The sensor chip unit 1300 is provided below the film-type unit 1100, and a reaction material that reacts with the target material may be fixed on the surface. Here, the reactant may be a biomaterial or a chemical substance.

The prism fixing unit 1400 is provided to closely contact the film-type unit 1100 and the prism unit 1200 and includes a first fixing part 1410, a second fixing part 1420 and an upper fixing part 1430 ).

The first fixing part 1410 is adapted to receive the prism unit 1200 and includes a first fixing body 1411, a first extension body 1412, a first mounting body 1413, a first light blocking inner surface 1414 A first transfer passage 1415, a first tube connection port 1416,

The first fixing unit 1411 is provided to be in close contact with a side surface of the prism unit 1200.

The first extension 1412 may extend from the first fixing unit 1411 so as to surround both sides of the prism unit 1200, and may be provided in a pair. That is, a pair of the first extension bodies 1412 may extend from both ends of the first fixing body 1411 so as to surround both sides of the prism unit 1200 adjacent to one side of the prism unit 1200.

The first mounting member 1413 may be stepped inward of the first fixing member 1411 so that one side of the prism unit 1200 is seated on the upper side. In other words, the first mounting member 1413 is formed so as to extend from the first fixing member 1411 in the direction of the second fixing unit 1420 so that a part of one side of the prism unit 1200 can be seated thereon And the first mounting member 1413 may be formed to have a step with the first fixing member 1411.

The first light blocking inner surface 1414 may be formed on the upper surface of the first fixing body 1411 and may have a downward inclined surface toward the incident surface 1220 of the prism unit 1200. The first light source inner surface 1414 thus formed is irradiated with light toward the incident surface 1220 so that the irradiated light is incident on the incident surface 1220 by the first fixing body 1411 Can be prevented from being blocked before reaching.

The first transfer path 1415 is formed in the first fixing unit 1411 and may be connected to the sample inlet 1240 of the prism unit 1200. A sealing member (not shown) may be formed between the first transfer path 1415 and the sample inlet 1240. Here, the sealing member includes a rubber ring, and the sealing member can prevent a fluid from leaking between the sample inlet 1240 and the transfer passage 1415.

The first tube connection port 1416 may be inserted into the first extension 1412 so that one side is connected to the tube and the other side is connected to the first transfer path 1415. In addition, the first tube fittings 1416 may be connected to the respective first extensions 1412 as shown, and may be provided in pairs.

The first threaded hole 1417 may be provided on the upper portion of the first extension 1412.

The second fixing part 1420 is positioned to face the first fixing part 1410 and the prism unit 1200 can be seated. The second fixing portion 1420 includes a second fixing body 1421, a second extension body 1422, a second mounting body, a second light inner surface 1424, a second transfer path 1425, A connecting hole 1426, and a second screw hole 1427.

The second fixing body 1421 is provided to be in close contact with the other side surface of the prism unit 1200.

The second extension 1422 may extend from the second fixing body 1421 to surround both sides of the other side of the prism unit 1200 and may be provided in a pair. That is, the pair of second extensions 1422 may extend from both ends of the second fixing body 1421 so as to enclose both sides of the prism unit 1200 adjacent to the other side of the prism unit 1200.

The second mount may be stepped inward of the second fixing body 1421 so that the other side of the prism unit 1200 is seated on the upper side. That is, the second mounting member may be provided on the upper portion of the second fixing member 1421 in a direction extending from the second fixing member 1421 toward the first fixing portion 1410 so that a portion of the other side of the prism unit 1200 may be seated. The second mounting body may be formed to have a stepped portion with the second fixing body 1421.

The second optical inner surface 1424 may be formed on the upper surface of the second fixing body 1421 and may have a downward inclined surface toward the reflecting surface 1230 of the prism unit 1200. The second light-blocking inner surface 1424 thus formed can prevent light reflected through the reflection surface 1230 from being blocked by the second fixing body 1421.

The second transfer path 1425 is formed inside the second fixing unit 1421 and may be connected to the sample outlet 1250 of the prism unit 1200. A sealing member (not shown) may be formed between the second transfer path 1425 and the sample outlet 1250. Here, the sealing body includes a rubber ring, and the sealing body can prevent a fluid from leaking between the sample outlet 1250 and the transfer passage 1425.

The second tube connection port 1426 may be inserted into the second extension 1422 so that one end thereof is connected to the tube and the other end thereof is connected to the second transfer path 1425. In addition, the second tube fittings 1426 may be connected to the respective second extensions 1422 as shown, and may be provided in pairs.

The second threaded hole 1427 may be provided on the upper portion of the second extension 1422.

The upper fixing part 1430 is coupled to the upper part of the first fixing part 1410 and the second fixing part 1420 to fix the prism unit 1200 and the first upper fixing part 1431 And a second upper fixing body 1432, as shown in Fig.

The first upper fixing body 1431 may be provided to connect the first fixing portion 1410 and the second fixing portion 1420.

More specifically, the first upper fixing body 1431 is fixed to the first extending portion 1412 of the first fixing portion 1410 and the second extending portion 1412 of the second fixing portion 1420, So that both ends thereof are located on the upper side. Screw connection holes may be formed at both ends of the first upper fixing body 1431 at positions corresponding to the first screw holes 1417 and the second screw holes 1427. [

The first upper fixture 1431 may be provided on a pair of the first extension 1412 and the second extension 1412 so as to be opposed to each other.

The second upper fixing body 1432 may extend to connect the pair of the first upper fixing bodies 1431. When the first upper fixing body 1431 is coupled to the first fixing portion 1410 and the second fixing portion 1420, the second upper fixing body 1432 is fixed to the prism unit 1200, So as to press against the lower part of the housing.

More specifically, an upper fixing screw 1433 may be inserted and coupled to each of the screw connection holes formed in the first upper fixing body 1431. The upper fixing screw 1433 may be inserted into and coupled to the screw connection hole, the first screw hole 1417, and the screw connection hole and the second screw hole 1427. As the upper fixing screw 1433 is inserted and tightened in the first screw hole 1417 and the second screw hole 1427, the second upper fixing body 1432 is inserted into the prism unit 1200, So that the prism unit 1200 can be pressed toward the film-type unit 1100. That is, depending on the degree of tightening of the upper fixing screw 1433, the degree of tightness between the prism unit 1200 and the film-type unit 1100 changes, and thus the airtightness may vary. Accordingly, when leakage occurs between the film type unit 1100 and the prism unit 1200, the upper fixing screw 1433 can be further tightened to easily prevent water leakage.

The sensor chip fixing unit 1500 includes a sensor chip fixing body 1510 and a sensor chip protrusion body 1520 which are provided to closely contact the film type unit 1100 and the sensor chip unit 1300.

The sensor chip fixing body 1510 may be inserted into the lower portion of the prism fixing unit 1400.

The sensor chip fixing body 1510 may be provided such that an upper edge portion of the sensor chip fixing body 1510 is engaged with the first mounting body 1413 and the second mounting body 1423 when inserted into the lower portion of the prism fixing unit 1400 . The lower surface of the prism fixing unit 1400 and the lower surface of the sensor chip fixing body 1510 are provided so as not to have a step when the sensor chip fixing body 1510 is inserted into the lower portion of the prism fixing unit 1400 .

In addition, a plurality of sensor chip screw holes 1530 may be formed in the sensor chip fixed body 1510. A lower fixing screw 1540 is inserted into the sensor chip screw hole 1530 to connect the sensor chip fixing body 1510 and the prism fixing unit 1400. Accordingly, the prism fixing unit 1400 may have a lower screw hole (not shown) formed at a position corresponding to the sensor chip screw hole 1530 in advance.

The sensor chip protrusion 1520 may be formed on the sensor chip fixing body 1510 and the film type unit 1100 and the sensor chip unit 1300 may be sequentially mounted on the sensor chip fixing body 1510.

The sensor chip protrusion 1520 may be coupled to the prism fixing unit 1400 so as to press the film-type unit 1100 and the sensor chip unit 1300 upward.

More specifically, the sensor chip protrusion 1520, the film unit 1100 and the sensor chip unit 1300 are fixed to the first and second seating members 1413 and 1413 of the prism fixing unit 1400 The thickness of the sensor chip protrusion 1520, the film unit 1100 and the sensor chip unit 1300 is located inside the first and second seating members 1413 and 1423, 1423, respectively.

The sensor chip protruding body 1520 is inserted into the screw hole provided in the sensor chip fixing body 1510 and the prism fixing unit 1400 so that the lower fixing screw 1540 is inserted into the screw hole provided in the prism fixing unit 1400, 1300 and the film-type unit 1100 by pressing the sensor chip unit 1300 and the film-type unit 1100 to the prism unit 1200.

Therefore, by tightening the upper fixing screw 1433 and the lower fixing screw 1540, the airtightness between the film-type unit 1110, the prism unit 1200, and the sensor chip unit 1300 .

When the sensor chip unit 1300 is to be replaced after the experiment, the lower head screw 1540 is loosened to separate the sensor chip fixing unit 1500 from the prism fixing unit 1400, The chip unit 1300 can be quickly and simply replaced.

FIG. 6 is a front view showing a path of light of a multi-channel microchannel measuring apparatus which can easily replace the sensor chip unit 1300 according to an embodiment of the present invention.

6, the polarization detection unit 1600 is configured to irradiate light toward the prism unit 1200 and detect a change in polarization of the reflected light, and the polarization detection unit 1600 includes a light source unit 1610, The lens unit 1620, the polarizer 1630, the analyzer 1640, and the optical detector 1650.

The light source unit 1610 is arranged to emit light toward the incident surface 1220 of the prism unit 1200.

The lens unit 1620 may be provided between the light source unit 1610 and the polarizer 1620 to receive light emitted from the light source unit 1610 and to provide parallel light to the polarizer 1620.

The polarizer 1630 may be provided to polarize the light emitted from the light source unit 1610.

The analyzer 1640 may be provided to polarize the reflected light that has passed through the reflecting surface 1230 of the prism unit 1200.

The light detecting unit 1650 may be provided to detect a change in polarization of the polarized reflected light.

The multi-channel microchannel measuring apparatus 1000, which is easy to replace the sensor chip unit as described above, is applicable to a biosensor device as an experimental equipment for developing a new drug.

FIG. 7 is a flowchart illustrating a method of measuring a multi-channel microchannel measuring apparatus according to an embodiment of the present invention. FIG.

7, a method of measuring a multi-channel microchannel measuring apparatus 1000 in which the sensor chip unit can be easily replaced includes the steps of injecting a sample solution into the film-type unit, And reacting the reaction material fixed on the sensor chip unit (S110).

In the step S110 of injecting a sample solution into the film-type unit and reacting a target material contained in the sample solution with a reaction material fixed to the sensor chip unit, a tube (not shown) is attached to the first tube connector 1416 ) Can be connected to inject the sample solution. The sample solution injected into the tube connection port 1416 may be transferred to the microfluidic channel 1120 through the first transfer path 1415 and the sample injection port 1240.

The target material contained in the sample solution transferred to the microfluidic channel 1120 may react with the reactive material fixed to the sensor chip unit 1300.

After injecting the sample solution into the film-type unit and reacting the target material contained in the sample solution with the reactive material fixed to the sensor chip unit (S110), the prism unit is irradiated with light to detect a change in polarization (S120) may be performed.

The light source unit 1610 of the polarization detection unit 1600 irradiates light toward the incident surface 1220 of the prism unit 1200 in a step S120 of detecting a change in polarization by irradiating the prism unit with light, can do. At this time, the irradiated light changes into parallel light while passing through the lens unit 1620, and the parallel light can be polarized through the polarizer 1630. The polarized light may be incident on the incident surface 1220. In this case, the prism fixing unit 1400 may include a first light-blocking inner surface 1414 to prevent light incident on the light incidence surface 1220 from being blocked.

The incident light incident through the incident surface 1220 is reflected by the fluid positioned in the microfluidic channel 1120 and the reflected light reflected from the fluid passes through the reflection surface 1230 to the analyzer 1640 Can be moved and polarized. The polarized light reflected from the analyzer 1640 can be detected by the optical detector 1650. That is, the optical detector 1650 can detect the change in polarization of the reflected light to obtain optical data and convert it into an electrical signal. The optical detector 1650 is provided with an analysis program that uses an reflectance measurement method and an ellipsometry method to extract and analyze optical data converted into an electrical signal to determine the absorption concentration of the sample solution, the thickness of the adsorption layer, A dissociation constant, a refractive index, and the like.

After the step S120 of detecting the polarization change by irradiating the prism unit with light, step S130 of injecting air into the film-type unit and discharging the sample solution may be performed.

Air may be injected into the first tube connection port 1416 in a step S130 of injecting air into the film-type unit and discharging the sample solution. The air injected into the tube connection port 1416 passes through the first transfer passage 1415 and the sample injection port 1240 and passes through the sample outlet 1250, The second transfer passage 1425, and the second tube connection port 1426, and can be easily discharged.

After the step S130 of injecting air into the film-type unit and discharging the sample solution, a step S140 of removing the sensor chip fixing unit and replacing the sensor chip unit may be performed.

In the step S140 of removing the sensor chip fixing unit and replacing the sensor chip unit, the sensor chip unit 1300 in which the reacted reaction material is fixed is separated from the sensor chip fixing unit, The new sensor chip unit 1300 can be coupled to the multi-channel microchannel measuring apparatus 1000, which facilitates replacement of the sensor chip unit.

It will be understood by those skilled in the art that the foregoing description of the present invention is for illustrative purposes only and that those of ordinary skill in the art can readily understand that various changes and modifications may be made without departing from the spirit or essential characteristics of the present invention. will be. It is therefore to be understood that the above-described embodiments are illustrative in all aspects and not restrictive. For example, each component described as a single entity may be distributed and implemented, and components described as being distributed may also be implemented in a combined form.

The scope of the present invention is defined by the appended claims, and all changes or modifications derived from the meaning and scope of the claims and their equivalents should be construed as being included within the scope of the present invention.

1000: Multichannel microchannel measurement device with easy replacement of sensor chip unit
1100: Film type unit 1110: Microfluidic film
1120: Microfluidic channel 1200: Prism unit
1210: prism body 1220: incident surface
1230: Reflecting surface 1240: Sample inlet
1250: sample outlet 1300: sensor chip unit
1400: prism fixing unit 1410: first fixing unit
1411: first fixing member 1412: first extension member
1413: first mounting member 1414: first mounting surface 1414:
1415: first transfer path 1416: first tube connecting port
1417: first screw hole 1420: second fixing portion
1421: second fixing body 1422: second extension body
1424: second light hole inner surface 1425: second transfer path
1426: second tube fitting 1427: second screw hole
1430: upper fixing part 1431: first upper fixing part
1432: second upper fixing body 1433: upper fixing screw
1500: Sensor chip fixing unit 1510: Sensor chip fixing structure
1520: Sensor chip protrusion 1530: Sensor chip screw hole
1540: Lower fixing screw 1600: Polarization detection unit
1610: Light source part 1620:
1630: Polarizer 1640: Analyzer
1650:

Claims (20)

A film type unit in which a microfluidic channel is formed through which a sample containing a target material can pass;
A prism unit provided on the film type unit and made of an optical prism;
A sensor chip unit provided at a lower portion of the film-type unit and having a surface on which a reaction material reacting with the target material is fixed;
A prism fixing unit provided so as to closely contact the film-type unit and the prism unit; And
And a sensor chip fixing unit provided so as to closely contact the film-type unit and the sensor chip unit,
Wherein the prism fixing unit and the sensor chip fixing unit press and fix the film type unit, the prism unit and the sensor chip unit so as to be in close contact with each other,
The prism fixing unit includes:
A first fixing part on which the prism unit is seated;
A second fixing part which is located opposite to the first fixing part and on which the prism unit is mounted; And
And an upper fixing unit coupled to an upper portion of the first fixing unit and the second fixing unit to fix the prism unit,
The upper fixing part
A pair of first upper fixing bodies connected to the first fixing portion and the second fixing portion; And
And a second upper fixing body extending to connect a pair of the first upper fixing bodies,
Wherein when the first upper fixing body is coupled to the first fixing portion and the second fixing portion, the second upper fixing body is configured to press the prism unit downward. Channel microchannel measurement device. The method according to claim 1,
In the film-type unit,
A microfluidic film made of a material having elasticity; And
And a microfluidic channel extending in the longitudinal direction of the microfluidic film and including at least one microfluidic channel. The method according to claim 1,
The prism unit includes:
A prism body forming a body and formed of an optical prism;
An incident surface formed on one side surface of the prism body and having an inclined surface; And
And a reflecting surface formed on the other side surface of the prism body and having an inclined surface,
Wherein the sensor chip unit is arranged such that incident light enters the incident surface and reflected light is reflected by the reflection surface. The method of claim 3,
Wherein the incident surface and the reflection surface are inclined surfaces having an inclination angle of 25 to 35 degrees. The method of claim 3,
The prism unit includes:
A sample inlet formed on one side of the prism body and formed below the incident surface; And
And a sample outlet formed on the other side of the prism body and formed on the lower side of the reflecting surface,
Wherein the sample inlet and the sample outlet extend toward the inside of the prism body to be connected to the microfluidic channel. delete The method according to claim 1,
Wherein the first fixing portion comprises:
A first fixture adapted to be in close contact with a side surface of the prism unit;
A pair of first elongated bodies extending from the first fixing body so as to surround both side surfaces of one side of the prism unit; And
And a first mounting member formed in a stepped manner in an inward direction of the first fixing member so that one side of the prism unit is seated. 8. The method of claim 7,
Wherein the first fixing portion comprises:
Further comprising a first light inner surface formed on an upper surface of the first fixed body and having a downward inclined surface toward an incident surface of the prism unit. . 8. The method of claim 7,
Wherein the first fixing portion comprises:
A first conveying passage formed inside the first fixing member and connected to a sample inlet of the prism unit; And
And a first tube connection port inserted into the first extension body and connected to the tube at one end and connected to the first transfer path at the other end of the sensor chip unit. Device. The method according to claim 1,
Wherein the second fixing portion comprises:
A second fixing body adapted to be in close contact with the other side surface of the prism unit;
A pair of second elongated bodies extending from the second fixing body so as to surround opposite side surfaces of the other side of the prism unit; And
And a second mounting member formed in a stepped manner in an inward direction of the second fixing member so that the other side of the prism unit is seated. 11. The method of claim 10,
Wherein the second fixing portion comprises:
Further comprising a second photocathode inner surface formed on the upper surface of the second fixing member and having a downward inclined surface toward a reflecting surface of the prism unit. . 11. The method of claim 10,
Wherein the second fixing portion comprises:
A second conveying passage formed inside the second fixing body and connected to a sample outlet of the prism unit; And
And a second tube connection port inserted into the second elongated body, one side of which is connected to the tube, and the other side of which is connected to the second transfer channel. Device. delete The method according to claim 1,
The sensor chip fixing unit includes:
A sensor chip fixed body inserted to a lower portion of the prism fixing unit; And
And a sensor chip protrusion formed on the sensor chip fixed body and on which the film-type unit and the sensor chip unit are mounted,
And the sensor chip unit is coupled to the prism fixing unit so as to press the film-type unit and the sensor chip unit upward. 15. The method of claim 14,
Wherein the sensor chip protruding body, the film-type unit, and the sensor chip unit are located inside the first and second mounting bodies of the prism fixing unit, Wherein the thickness of the unit is the same as the thickness of the first and second seating members. The method according to claim 1,
Further comprising a polarization detection unit configured to irradiate light toward the prism unit and detect a change in polarization of the reflected light, wherein the sensor chip unit is easy to replace. 17. The method of claim 16,
The polarization detection unit includes:
A light source for emitting light toward an incident surface of the prism unit;
A polarizer for polarizing the light emitted from the light source unit;
A lens unit receiving light emitted from the light source unit and providing parallel light to the polarizer;
An analyzer for polarizing the reflected light that has passed through the reflection surface of the prism unit;
And a photodetector for detecting a change in polarization of the polarized reflected light, wherein the sensor chip unit is easy to replace. A biosensor device using the multi-channel microchannel measuring device according to claim 1, wherein the sensor chip unit can be easily replaced. A method of measuring a multi-channel microchannel measuring apparatus according to claim 1, wherein the sensor chip unit is easy to replace,
a) injecting a sample solution into the film-type unit, and reacting a target material contained in the sample solution with a reaction material fixed to the sensor chip unit;
b) illuminating the prism unit with light to detect a polarization change; And
and c) injecting air into the film-type unit to discharge the sample solution. A method of measuring a multi-channel microchannel measurement apparatus, comprising: 20. The method of claim 19,
After step c)
and d) removing the sensor chip fixing unit to replace the sensor chip unit, wherein the sensor chip unit is easily replaced.
KR1020170131673A 2017-10-11 2017-10-11 Multi-channel microchannel measuring apparatus which can easily replace sensor chip unit and method for measuring the same KR101971634B1 (en)

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