KR101971265B1 - Strain for decomposing tetramethylammonium hydroxide and method for water treatment using thereof - Google Patents

Abstract

The present invention provides a strain of Gordonia cholesterolivorans HTS-S2 (KCTC13663BP) which is capable of decomposing tetramethylammonium hydroxide (TMAH), a composition containing the same, and a method for decomposing TMAH using the same.

Description

TECHNICAL FIELD The present invention relates to a strain for decomposing tetramethylammonium hydroxide and a method for treating water using the same,

The present invention relates to a strain capable of degrading tetramethylammonium hydroxide, a composition comprising the same, and a method for decomposing tetramethylammonium hydroxide using the same.

The etchant used for photolithography contains tetramethylammonium hydroxide (TMAH), which is toxic, and the problem is how to properly treat waste water containing etchant. In order to solve this problem, there are known methods such as an adsorption method, an ion exchange method, an ultrafiltration method, a method of concentrating and disposing by reverse osmosis method, or a supercritical combustion treatment method of concentrated TMAH. However, these chemical processes require complicated steps, There is a problem that tea contamination occurs.

Accordingly, biological processes using microorganisms capable of decomposing continuously available TMAH while reducing environmental pollution are being studied. For example, Korean Patent Laid-Open No. 10-2002-0009060 discloses a method of decomposing TMAH using strains IBN-H1 to IBN-H7 in the genus Ashinobacterium. However, strains IBN-H1 to IBN-H7 have a low TMAH resolution when applied on a large scale, making it difficult to apply them to actual industrial fields.

The inventors of the present invention sought to find microorganisms having high utilization value because they have a high TMAH resolution even when decomposing TMAH contained in large scale wastewater.

Korean Patent Publication No. 10-2002-0009060

Keiji Hirano, et al., An Efficient Treatment Technique for TMAH Wastewater by Catalytic Oxidation, IEEE TRANSACTIONS ON SEMICONDUCTOR MANUFACTURING, VOL. 14, NO. 3, AUGUST 2001, pp. 202-206

The present invention provides a Gordonia cholesterolivorans HTS-S2 (KCTC13663BP) strain having TMAH resolution, a composition for degrading TMAH containing the same, a porous carrier containing the same, and a water treatment method using the same .

Item 1. Gordonia cholesterolivorans HTS-S2 (KCTC13663BP) with resolution of tetramethylammonium hydroxide (TMAH).

Item 2. A composition for degrading TMAH comprising the strain of item 1.

The method according to item 3. Item 2, Acinetobacter in Strain IBN-H6 (Acinetobacter sp IBN- H6;. KCTC 10809BP), Acinetobacter genus IBN-H7 (Acinetobacter sp IBN- H7;. KCTC 0836BP), Clube My process Delphi four cis IBN-H1 (Kluvermyces delphenisis IBN- H1; KCTC 0834BP), Bacillus cereus IBN-H4 (Bacillus cereus IBN- H4;. KCTC 0835BP), Mycobacterium species SMIC-1 (Mycobacterium sp SMIC- 1 KCTC 10947 BP) and a bacterium of methyl Solarium X Toku Enschede SMIC-1 ( Methylobacterium extorquens SMIC-1 KCTC 10946 BP). ≪ / RTI >

Item 4. A porous carrier comprising the composition for degrading TMAH of item 2.

Item 5. The porous carrier according to item 5, wherein the carrier is a hexahedral polyurethane foam.

Item 6. A microbial water treatment method comprising contacting a wastewater comprising TMAH with a strain of Item 1, a composition of Item 2 or 3, or a porous carrier of Item 4 or 5.

Item 7. The method according to item 6, wherein said carrier is selected from the group consisting of Strain IBN-H6 (Acinetobacter sp IBN- H6;. KCTC 10809BP), Acinetobacter genus IBN-H7 (Acinetobacter sp IBN- H7;. KCTC 0836BP), Clube My process Delphi four cis IBN-H1 (Kluvermyces delphenisis IBN- H1; KCTC 0834BP), Bacillus cereus IBN-H4 (Bacillus cereus IBN- H4;. KCTC 0835BP), Mycobacterium species SMIC-1 (Mycobacterium sp SMIC- 1 KCTC 10947 BP) and a bacterium of methyl Solarium X Toku Enschede SMIC-1 ( Methylobacterium extorquens SMIC-1 KCTC 10946 BP). ≪ / RTI >

Item 8. The microbial water treatment method according to item 6, wherein the microbial water treatment is carried out under aerobic conditions at 15 ° C to 42 ° C.

Item 9. The microorganism according to item 8, wherein the TMAH of the influent wastewater is 4 to 7 g / L, the pH of the reaction tank is adjusted to a range of 5-8, and the operation is continued under the condition of a residence time of 24-120 hours Water treatment method.

By using the strain Gordonia cholesterolivorans HTS-S2 (KCTC13663BP) according to the present invention, TMAH can be degraded with high efficiency. The strain according to the present invention can be applied to a known biological wastewater treatment process, and the decomposition efficiency of TMAH can be increased by using the composition or the porous carrier containing the strain according to the present invention. By using the strain according to the present invention, TMAH can be decomposed by 90% or more, and it is environmentally friendly since no additional compound is added. Also, the TMAH decomposition method using the microorganism contained in the composition or the porous carrier according to the present invention can decompose a high concentration of TMAH into a large amount, and the process is simple, and the installation and maintenance cost is low and the operation is easy.

FIG. 1 is a result of analyzing 16s RNA full-length sequence of a strain according to an embodiment of the present invention.
FIG. 2 is a result of analysis of a strain according to an embodiment of the present invention by MALDI-TOF.
FIG. 3 is a result of analyzing a strain according to an embodiment of the present invention with an API kit.
FIG. 4 shows the result of microscopic observation of a strain according to an embodiment of the present invention.
5 is a schematic view showing a process of decomposing TMAH in a large capacity using a porous carrier containing microorganisms according to an embodiment of the present invention.

BRIEF DESCRIPTION OF THE DRAWINGS The present invention will now be described more fully hereinafter with reference to the accompanying drawings, but only some of the embodiments of the invention are not exhaustive of the invention. Indeed, these inventions may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. The singular forms as used in this specification and the appended claims include plural referents unless the context clearly dictates otherwise.

Tetramethylammonium hydroxide (TMAH) is a quaternary ammonium salt having the molecular formula N (CH ₃ ) ₄ ⁺ OH ^- , having a melting point of about 67 ° C. It is used to anisotropically etch silicon, and is strongly basic. It is known that TMAH decomposition is difficult and has high toxicity (Izumi C. Mori, et al., Toxicity of tetramethylammonium hydroxide to aquatic organisms and its synergistic action with potassium iodide, Chemosphere, vol. 120, pp. 299-304, 2015 ).

Microorganisms having TMAH resolution and compositions comprising same

The present invention discloses a strain capable of degrading tetramethylammonium hydroxide (TMAH), Gordonia cholesterolivorans HTS-S2 (KCTC13663BP), which is a strain capable of degrading tetramethylammonium hydroxide (TMAH).

Further, the above-mentioned Gordonia cholesterol riborans HTS-S2 ( Gordonia cholesterolivorans HTS-S2) (KCTC13663BP). ≪ / RTI > In one embodiment of the present invention, the composition may further include a medium, a preservative, or a buffer, and is capable of providing an environment for maintaining the survival and / or TMAH degrading activity of the microorganism. Further, in one embodiment of the present invention, the composition may be in the form of activated sludge.

In one embodiment of the invention, the composition may comprise an active ingredient with TMAH resolving power isolated from the strain or strain in question. The " composition " of the present invention is referred to herein as including the above case where the same effect can be obtained, unless otherwise defined. The active substance may be an enzyme or a protein, but is not limited thereto.

In one embodiment of the present invention, the composition may further comprise a known strain capable of degrading TMAH. TMAH degrading microorganisms which may be used in combination in the composition according to the invention, for example, but are not limited to, Acinetobacter genus Strain IBN-H6 (Acinetobacter sp IBN- H6;. KCTC 10809BP), Acinetobacter genus IBN-H7 (Acinetobacter sp IBN- H7;. KCTC 0836BP), Clube My process Delphi four cis IBN-H1 (Kluvermyces delphenisis IBN- H1; KCTC 0834BP), Bacillus cereus IBN-H4 (Bacillus cereus IBN- H4;. KCTC 0835BP), Mycobacterium species SMIC-1 (Mycobacterium sp SMIC- 1 KCTC 10947 BP) and a bacterium of methyl Solarium X Toku Enschede SMIC-1 ( Methylobacterium extorquens SMIC-1 KCTC 10946 BP).

In one embodiment of the present invention, Gordonia cholesterol riborans HTS-S2 ( Gordonia cholesterolivorans HTS-S2) (KCTC13663BP), or a method for degrading TMAH using a carrier comprising the strain. The method comprises biodegrading TMAH by mixing a composition comprising the strain, or a carrier comprising the strain, with a solution comprising TMAH. The method is preferably carried out under aerobic conditions and may be carried out at 4 ° C to 42 ° C, or 15 ° C to 42 ° C, unless the TMAH degrading activity of the active ingredient is eliminated.

The term " strain " as used herein means a genetically identical population that has grown in one cell.

Decomposition of TMAH

It is possible to decompose TMAH from the wastewater containing the step of bringing the microorganism, the composition containing the same, or the porous carrier according to the present invention into contact with wastewater containing TMAH. The TMAH decomposition process may be included as a first step in a known wastewater treatment process, as an intermediate process, or as a final process, and the timing of the TMAH decomposition process may be changed according to the purpose.

The water treatment method is described in Korean Patent No. 10-0823149. More specifically, the water treatment can be performed using a fluidized bed biofilm reaction apparatus for waste water treatment including a raw water tank, a feed pump, a reaction tank, a flocculation tank, and a pressurized floating tank. The reaction tank has a hexagonal porous carrier filled with 30 to 50% of the apparent volume of the whole reaction tank, and has a wedge wire screen, and the pressurized floating tank is installed at the rear end of the flocculation reaction tank. The reaction tank is a system in which the contaminants in the waste water flowing from the raw water tank are brought into contact with the microorganisms attached to the porous carrier and mixed with air using a diffuser to convert the organic matter into the microorganism body, By converting to metabolic by-products, it plays a role of removing pollutants in waste water and wastewater. The reaction tank is capable of both single side aeration and front side aeration. The wedge wire screen is installed perpendicular to the reaction vessel wall to separate the porous carrier of cubic type from the treated water. The wedge wire screen is structured so that the obstruction of the screen can be visually confirmed, and the screen for cleaning the screen is installed at a proper distance so that the air can be cleaned. The excess sludge generated by the biodegradation is continuously discharged from the reaction tank due to friction due to the flow of the carrier.

Cultivation of microorganisms

The term " cultivation " as used herein means a process of growing and propagating microorganisms. The culturing method is well known in the art, and any person skilled in the art can choose according to the type of microorganism. In one embodiment of the present invention, the microorganism having TMAH resolving ability can be cultured according to a liquid culture method or a solid culture method, and the liquid culture method can be, for example, a surface culture method, a deep culture method or a shaking culture method, But is not limited to, for example, stationary culture, aeration culture, and fluidized bed culture. The microorganism according to the present invention is preferably cultured under aerobic conditions.

The term " medium " as used herein means a composition in which a nutrient required by a microorganism is artificially formed in order to cultivate the microorganism. The medium may be a solid or a liquid, and the solid medium may be prepared by adding agar, gelatin, silica gel or the like to the liquid medium to solidify the medium.

The term " inoculation " as used herein means injecting the medium to start cell culture and injecting cells into the injected medium. Here, the medium may be injected before the cells are injected or injected into the cell reactor simultaneously with the cells.

Porous carrier

The Gordonia cholesterol riborans HTS-S2 & lt ; RTI ID = 0.0 & gt ; ( Gordonia cholesterolivorans HTS-S2) (KCTC13663BP) microorganism may be contained in a porous carrier for carrying microorganisms.

The porous carrier for transporting microorganisms may be any porous carrier used in the art, including sponge-like synthetic resin foams, and porous polyurethane foams. The carrier may be, for example, but not limited to, polyurethane, poly (ethylene glycol) monoacrylate, poly (ethylene glycol) diacrylate, poly (ethylene glycol) monomethacrylate, poly (Ethylene glycol) methyl ether acrylate, poly (ethylene glycol) methyl ether methacrylate, poly (ethyleneglycol) divinyl ether, poly (ethylene glycol) ethyl ether methacrylate, (Ethyleneglycol) urethane diacrylate, poly (ethylene glycol) urethane methacrylate, poly (ethylene glycol) urethane dimethacrylate, poly (ethylene glycol) urethane monoacrylate, poly ) Urethane triacrylate, poly (ethylene glycol) urethane trimethacrylate, Poly (ethylene-co-propylene glycol) monomethacrylate, poly (ethylene-co-propylene glycol) methyl ether methacrylate, (Ethylene-co-propylene glycol) methyl ether acrylate, poly (ethylene-co-propylene glycol) ethyl ether acrylate, and Polyalkylene glycol-based polymer backbone formed by polymerization of at least one polyalkylene glycol-based precursor selected from the group consisting of poly (ethylene-co-propylene glycol) butyl ether acrylate, A polyalkylene glycol-based polymer having a three-dimensional network structure may be used. In one embodiment of the present invention, the carrier is preferably a porous polyurethane foam carrier.

Particularly, a method for producing the above porous polyurethane foam carrier is described in Korean Patent No. 10-0823151 entitled " Porous Polyurethane Foam Support and Preparation Process and Aeration Tank for Fluidized Biofilm Reactor ". (A) an isocyanate compound selected from the group consisting of i) 4,4-diphenylmethane diisocyanate, 2,4-toluene-diisocyanate, 2,6-toluene-diisocyanate and mixtures thereof, ii Iii) a catalyst selected from the group consisting of dimethylcyclohexylamine, tetramethylhexanediamine, pentamethylenediethylenetriamine and triethylenediamine; iv) a catalyst selected from the group consisting of Si-C bonds or Si-OC bonds And v) blowing agents selected from the group consisting of fluorocarbons (HFC), chlorofluoro-hydrocarbons (HCFC) and cyclopentane are introduced into the mixing head through respective independent lines through a mixing head ) To obtain a sponge-like porous polyurethane foam carrier; (b) Horizontal axis circular rotary knife bundle, in which circular knives are arranged at equal intervals in a range of 3 to 4.5 mm on a shaft, is vertically moved to vertically cut a sponge-like porous polyurethane foam carrier, Expelling step; (c) horizontally moving a vertical shaft circular rotary knife bundle having circular knives arranged at regular intervals in a range of 3 to 4.5 mm on a shaft, horizontally cutting a vertically sheathed sponge-like porous polyurethane foam carrier A step of forming an equal interval sheath; (d) finishing and cutting using a band knife to obtain a porous polyurethane foam carrier in the form of a uniform hexahedron in the range of 3 to 4.5 mm in height, width and height; (e) suctioning a vacuum on a porous polyurethane carrier in the form of a cut hexahedron using a carrier suction pump, and then removing the debris in two steps using a mesh net And can be manufactured by a person skilled in the art in an easily deformable manner. It is preferable that the porous carrier produced by the above method does not contain an active carbon component or an adhesive resin component.

Identification of microorganisms

16s rRNAs contain highly conserved primer binding sites and contain species-specifically hypervariable regions, and are therefore used to identify bacteria. 16s rRNA sequence analysis was carried out using 8F (5'-AGAGTTTGATCCTGGCTCAG-3 '), U1492R (5'-GGTTACCTTGTTACGACTT-3'), 928F (5'-TAAAACTYAAAKGAATTGACGGG- 3 '), 336R (5'- ACTGCTGCSYCCCGTAGGAGTCT- (5'-GAGATTAGATACCCTGGTA-3 '), 1100R (5'-GGGTTGCGCTCGTTG-3'), 337F (5'-GACTCCTACGGGAGGCWGCAG-3 '), 907R (5'- CCGTCAATTCCTTTRAGTTT- 3 '), 275 (5'-AGAGTTTGATCMTGGCTCAG-3'), or 1492R (5'-GACTACCAGGGTATCTAATC- (5'-CGGTTACCTTGTTACGACTT-3 '), or a combination thereof. The genome can be PCR-amplified using the primer pairs described above, and the amplified DNA sequence can be analyzed to identify the bacterium using the rRNA database and sequencing software provided by NCBI. Comparison of gene sequences can be performed by manual evaluation of sequences by those skilled in the art or by BLAST (Basic Local Alignment Search Tool; Altschul et al., J. Mol. Biol. 215: 403 (1993) ; (available at ncbi.nlm.nih.gov/BLAST/) by computer-automated sequence comparison and verification. The term " sequencing software " is any computer algorithm or software program useful for analyzing gene sequences. &Quot; Sequencing software " is commercially available or can be developed independently. A typical sequence analysis software is the GCG program suite (Wisconsin Package Version 9.0, Genetics Computer Group (GCG), Madison, WI), BLASTP, BLASTN, BLASTX (Altschul et al., J. Mol. Biol. 215: 403 And DNASTAR (DNASTAR, Inc. 1228 S. Park St. Madison, Wis. 53715 USA). Within the context of the present application, the analysis results, which are not otherwise specified when sequencing software is used for analysis, will be understood to be based on the "basic parameters" of the displayed program. Quot; default value " as used herein shall mean any set of numerical values or parameters contained in the original software at the start of the process.

The API 50 CH strip consists of 50 microtubes, each of which can be tested for anaerobic zones for fermentation testing and aerobic zones for oxidation and assimilation testing to check for carbohydrate metabolism in microorganisms. The fermentation is confirmed by the color change in the tube part, and the pH indicator shows the change caused by the acid generated in the anaerobic condition.

The API 20 E strip consists of 20 tubes containing a dried substrate. When the test tubes are inoculated with the bacterial suspension and incubated, the color is changed by the reaction products generated during the incubation time, or the color is changed by the addition of the auxiliary reagent, and the result is read. Each tube contains dehydrated media containing a chemically defined composition for each test.

The following examples are intended for illustrative purposes only and are not intended to limit the scope of the invention in any way.

Example

Example 1. Isolation of TMAH-degrading strain

250 ml of the waste water sample was put into a sterilized side filter bag and the sample was homogenized using a stomacher. 1 ml of the homogenized sample is inoculated into 9 ml of sterile physiological saline to dilute the sample 10 times and 0.3 ml of the diluted sample is inoculated into 1X TSA (15 g Trypton, 5 g Soytone, 5 g sodium chloride, 15 g Agar / L) and 0.1X TSA After culturing, the cells were cultured in an incubator at 30 DEG C for 5 days. After culturing, the colonies were selected and cultured separately in TSA. Each strain was transferred to a cryovial and stored frozen according to the usual method. The frozen strains were then used in the experiment.

Each of the obtained strains was inoculated into a TSA solid medium containing 0.01% (w / v) of TMAH and 0.2% (w / v), and cultured at 30 ° C for 24 hours. Thirteen strains cultivated in a minimal medium containing 0.01% (w / v) TMAH were selected, and 13 strains were selected by reclassifying microorganisms with similar morphology, and 0.2% (w / v) Were selected from 10 strains cultured in the TSA solid medium.

Example 2. Identification of TMAH decomposition activity

A total of 23 microorganisms selected in Example 1 were cultured in a minimal medium containing 0.01% (w / v) of TMAH at 30 ° C for 24 hours, and then the concentration of TMAH remaining in the medium was measured 1 and Table 2). The control group was not inoculated with any microorganisms and maintained for the same period of time under the same conditions as the experimental group.

Sample TMAH (mg / L) Control group 111 6 82.9 15 82.4 15d 85.2 21b * N.D. 24 N.D. 37 N.D. 37d N.D. 44a 86 51 72.9 62 71.1 64 N.D. 78 N.D. 82 N.D.

* N.D .; Not detected; Not detected

Sample TMAH (mg / L) Control group 112 1-1 * N.D. 1-2 N.D. 1-6 N.D. 1-7 N.D. 1-12 N.D. 2-3 N.D. 2-9 N.D. 2-10 N.D. 2-11 N.D. 2-17 N.D.

* N.D .; Not detected; Not detected

Example 3. Identification of TMAH-degrading microorganisms

3.1 16s rRNA Sequence Analysis

Of the microorganisms of Example 2, 11 microorganisms were selected by sorting microorganisms having similar TMAH degradation efficiencies and 16s rRNA sequences were analyzed using 27F and 1492R primers: 27F: 5'-AGA GTT TGA TCM TGG CTC AG-3 '; 1492R: 5'-CGG TTA CCT TGT TAC GAC TT-3 '(Jiang H, Dong H, Zhang G, Yu B, Chapman LR, Fields MW Applied and Environmental Microbiology, 72 (6): 3832-45.). Each microorganism was identified from the sequence analyzed using the MicroSeq 500 microbial identification system, and the results are shown in Table 3. SEQ ID Nos: 1, 2, and 3 are the 16s rRNA sequence analysis results of samples 2-10, 24, and 64, respectively (FIGS.

Sample result 24, 37 Lysinibacillus sphaericus 78, 82, 2-10 Stenotrophomonas maltophilia 37d, 64 Gordonia cholesterolivorans 1-6, 2-11 Bacillus cereus 1-7, 21b Bacillus wiedmannii

3.2 MALDI-TOF Analysis

Samples 24, 2-10, and 64 were each subjected to MALDI-TOF analysis. More specifically, 300 μl of distilled water and 20 μl of bacteria were mixed and stirred in an Eppendorf tube, heated at 100 ° C. for 30 minutes, and centrifuged at 13,000 rpm for 30 seconds to remove the supernatant. 900 쨉 l of 100% ethanol was added to the precipitate, stirred, centrifuged at 13,000 rpm for 2 minutes, and the supernatant was removed. After centrifuging once again under the same conditions as above, the remaining solution was removed and the pellet was dried at room temperature. The beads were poured in the same amount as the dried pellets, and the same amount of 20 μl of acetonitrile was added, followed by stirring for 1 minute. 20 mu l of 70% formic acid was added, stirred for 5 seconds, and centrifuged at 13,000 rpm for 2 minutes. 1 상 of the supernatant was loaded on the plate and dried at room temperature. Pretreatment samples were coated with 1 α of α-cyano-4-hydroxycinnamic acid (matrix solution) and measured by MALDI-TOF MS. The instrument ionizes the specimen by laser irradiation, and the molecular weight is measured by measuring the time the ions reach the detector according to the amount of charge.

The results are shown in Fig. As a result, Sample 2-10 (FIG. 2A) was identified as Stenotrophomonas maltophilia , and Sample 24 (FIG. 2B) was identified as Lysinibacillus sphaericus . Sample 64 (FIG. 2C) was unreadable because there was no data in the data sheet in the MALDI-TOF.

3.3 API Kit Analysis

Samples 24, 2-10, and 64 were analyzed according to the manufacturer's instructions using API 20E strips and API 50CH (BIOMERIEUX), respectively.

The results are shown in Fig. 3A (sample 24), Fig. 3B (sample 2-10) and Fig. 3C (sample 64).

3.4 Morphological analysis and others

Samples 24, 2-10, and 64 were observed with microscopic and microscopic microbial morphology and motility, respectively, and analyzed by Gram stain.

The strains 2-10 (FIGS. 4A and 4B) were motile, rod-shaped, and aerobic microorganisms corresponding to Gram-negative bacteria. 24 strains (Figures 4c and 4d) were aerobic microorganisms corresponding to rod-shaped Gram-negative bacteria with motility, and confirmed endospore. 64 strains (Figs. 4E and 4F) were aerobic microorganisms corresponding to rod-shaped Gram-negative bacteria with motility, and were able to degrade cholesterol.

Examples 3.3 and 3.4 also results as in the Example 3.1 of the 16s rRNA analysis of the samples 2-10 to note stacking Pomona's malto pilriah (Stenotrophomonas maltophilia), sample 24 is a license shinny Bacillus spare Li carcass (Lysinibacillus sphaericus), Sample 64 Gordonia cholesterolivorans . ≪ / RTI >

The microorganisms identified as above, Lysinibacillus sphaericus HTS-S3, Stenotrophomonas maltophilia HTS-S1 and Gordonia cholesterolivorans HTS-S2 were deposited at the Korea Biotechnology Research Center.

Example 4. Treatment of wastewater containing TMAH using microorganisms

Pick Macedonia cholesteryl as ribonucleic lance HTS-S2 (Gordonia cholesterolivorans HTS- S2) (KCTC13663BP), Lai shinny Bacillus spare Li coarse strain HTS-S3 (Lysinibacillus sphaericus HTS- S3) (KCTC13664BP), and a stacking notes Pomona's malto pilriah HTS-S1 The degradation efficiency of TMAH contained in wastewater was measured using a bioreactor filled with a porous carrier containing Stenotrophomonas maltophilia HTS-S1 (KCTC13662BP).

The porous carrier was contacted while supplying wastewater containing 4,000 to 7,000 mg / L of TMAH at about 250 m ³ / day, reacted in aeration tank at a pH of 5 to 8, and at a temperature of 15 to 42 ° C with stirring, The TMAH concentrations before and 1 to 5 days after the reaction were measured (Table 4).

division TMAH (mg / L) Enforcement 1 enemy 5300 Treated water N.D. Enforcement 2 enemy 5300 Treated water N.D. Enforcement 3 enemy 4094 Treated water N.D. Enforcement 4 enemy 5513 Treated water 129 Enforcement 5 enemy 5512 Treated water N.D. Enforcement 6 enemy 5506 Treated water 76

* N.D .; Not detected; Not detected

TMAH was contained at an average of about 5204 mg / L as the influent source, and about 99.4% of the TMAH was decomposed on an average basis by using the microorganism according to the present invention. According to the present invention, even when a large-scale waste water treatment, are included in the porous carrier, Lai shinny Bacillus spare Li coarse HTS-S3 strain (Lysinibacillus sphaericus) HTS-S3 ( KCTC13664BP), a stacking notes Pomona's malto pilriah HTS-S1 Stenotrophomonas maltophilia HTS-S1 (KCTC13662BP) and Gordonia cholesterolivorans HTS-S2 (KCTC13663BP) showed an average TMAH resolution of about 98% or more.

For example, for purposes of constituting a claim, the claims set forth below should not be construed in any way as narrower than its literal language, and thus the illustrative embodiment from the specification should not be read as a claim. It is, therefore, to be understood that the present invention has been described by way of example and not limitation in the scope of the claims. Accordingly, the invention is limited only by the following claims. All publications, published patents, patent applications, publications, and journal articles cited in this application are herein incorporated by reference in their entirety.

Korea Biotechnology Research Institute KCTC13662BP 20181018 Korea Biotechnology Research Institute KCTC13663BP 20181018 Korea Biotechnology Research Institute KCTC13664BP 20181018

<110> Hansu Technical Service LTD <120> Strain for decomposing tetramethylammonium hydroxide <130> 18P10017 <160> 3 <170> KoPatentin 3.0 <210> 1 <211> 1469 <212> DNA <213> Stenotrophomonas maltophilia <220> <221> rRNA &Lt; 222 > (1) .. (1469) <223> 16s RNA full sequence <400> 1 gctcagagtg aacgctggcg gtaggcctaa cacatgcaag tcgaacggca gcacaggaga 60 gcttgctctc tgggtggcga gtggcggacg ggtgaggaat acatcggaat ctactctgtc 120 gtgggggata acgtagggaa acttacgcta ataccgcata cgacctacgg gtgaaagcag 180 gggatcttcg gaccttgcgc gattgaatga gccgatgtcg gattagctag ttggcggggt 240 aaaggcccac caaggcgacg atccgtagct ggtctgagag gatgatcagc cacactggaa 300 ctgagacacg gtccagactc ctacgggagg cagcagtggg gaatattgga caatgggcgc 360 aagcctgatc cagccatacc gcgtgggtga agaaggcctt cgggttgtaa agcccttttg 420 ttgggaaaga aatccagccg gctaatacct ggttgggatg acggtaccca aagaataagc 480 accggctaac ttcgtgccag cagccgcggt aatacgaagg gtgcaagcgt tactcggaat 540 tactgggcgt aaagcgtgcg taggtggtta tttaagtccg ttgtgaaagc cctgggctca 600 acctgggaac tgcagtggat actggatgac tagaatgtgg tagagggtag cggaattcct 660 ggtgtagcag tgaaatgcgt agagatcagg aggaacatcc atggcgaagg cagctacctg 720 gaccaacatt gacactgagg cacgaaagcg tggggagcaa acaggattag ataccctggt 780 agtccacgcc ctaaacgatg cgaactggat gttgggtgca atttggcacg cagtatcgaa 840 gctaacgcgt taagttcgcc gcctggggag tacggtcgca agactgaaac tcaaaggaat 900 tgacgggggc ccgcacaagc ggtggagtat gtggtttaat tcgatgcaac gcgaagaacc 960 ttacctggcc ttgacatgtc gagaactttc cagagatgga ttggtgcctt cgggaactcg 1020 aacacaggtg ctgcatggct gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc 1080 aacgagcgca acccttgtcc ttagttgcca gcacgtaatg gtgggaactc taaggagacc 1140 gccggtgaca aaccggagga aggtggggat gacgtcaagt catcatggcc cttacggcca 1200 gggctacaca cgtactacaa tggtagggac agagggctgc aagccggcga cggtaagcca 1260 atcccagaaa ccctatctca gtccggattg gagtctgcaa ctcgactcca tgaagtcgga 1320 atcgctagta atcgcagatc agcattgctg cggtgaatac gttcccgggc cttgtacaca 1380 ccgcccgtca caccatggga gtttgttgca ccagaagcag gtagcttaac cttcgggagg 1440 gcgcttgcca cggtgtggcc gatgactgg 1469 <210> 2 <211> 1439 <212> DNA <213> Unknown <220> <223> Lysinibacillus sphaericus <220> <221> rRNA &Lt; 222 > (1) .. (1439) <223> 16s RNA full sequence <400> 2 tggctcagga cgaacgctgg cggcgtgcct aatacatgca agtcgagcga acagataagg 60 agcttgctcc tttgacgtta gcggcggacg ggtgagtaac acgtgggcaa cctaccctat 120 agtttgggat aactccggga aaccggggct aataccgaat aacttgttgt ctctcatgag 180 acaattctaa aagacggttt cggctgtcgc tataggatgg gcccgcggcg cattagctag 240 ttggtgaggt aacggctcac caaggcgacg atgcgtagcc gacctgagag ggtgatcggc 300 cacactggga ctgagacacg gcccagactc ctacgggagg cagcagtagg gaatcttcca 360 caatgggcga aagcctgatg gagcaacgcc gcgtgagtga agaaggattt cggttcgtaa 420 aactctgttg taagggaaga acaagtacag tagtaactgg ctgtaccttg acggtacctt 480 attagaaagc cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcaagcgt 540 tgtccggaat tattgggcgt aaagcgcgcg caggtggttt cttaagtctg atgtgaaagc 600 ccacggctg accgtggagg gtcattggaa actgggagac ttgagtgcag aagaggatag 660 tggaattcca agtgtagcgg tgaaatgcgt agagatttgg aggaacacca gtggcgaagg 720 cgactatctg gtctgtaact gacactgagg cgcgaaagcg tggggagcaa acaggattag 780 ataccctggt agtccacgcc gtaaacgatg agtgctaagt gttagggggt ttccgcccct 840 tagtgctgca gctaacgcat taagcactcc gcctggggag tacggtcgca agactgaaac 900 tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac 960 gcgaagaacc ttaccaggtc ttgacatccc attgaccact gtagagatac agttttccct 1020 tcggggacaa cggtgacagg tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg 1080 gttaagtccc gcaacgagcg caacccttga tcttagttgc catcatttag ttgggcactc 1140 taaggtgact gccggtgaca aaccggagga aggtggggat gacgtcaaat catcatgccc 1200 cttatgacct gggctacaca cgtgctacaa tggacgatac aaacggttgc caactcgcga 1260 gagggagcta atccgataaa gtcgttctca gttcggattg taggctgcaa ctcgcctaca 1320 tgaagccgga atcgctagta atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc 1380 ttgtacacac cgcccgtcac accacgagag tttgtaacac ccgaagtcgg tgaggtaac 1439 <210> 3 <211> 1409 <212> DNA <213> Unknown <220> <223> Gordonia cholesterolivorans <220> <221> rRNA &Lt; 222 > (1) .. (1409) <223> 16s RNA full sequence <400> 3 tggctcagga cgaacgctgg cggcgtgctt aacacatgca agtcgaacgg aaaggcccag 60 cttgctgggt actcgagtgg cgaacgggtg agtaacacgt gggtgatctg ccctggactc 120 tgggataagc ctgggaaact gggtctaata ccggatagga ccactgattg catggttggt 180 ggtggaaagc ttttgcggtt caggatgggc ccgcggccta tcagcttgtt ggtggggtaa 240 tggcctacca aggcgacgac gggtagccga cctgagaggg tgatcggcca cactgggact 300 gagacacggc ccagactcct acgggaggca gcagtgggga atattgcaca atgggcgcaa 360 gcctgatgca gcgacgccgc gtgagggatg acggccttcg ggttgtaaac ctctttcgct 420 agggacgaag cgtaagtgac ggtacctgga gaagaagcac cggccaacta cgtgccagca 480 gccgcggtaa tacgtagggt gcgagcgttg tccggaatta ctgggcgtaa agagctcgta 540 ggcggtttgt cgcgtcgtct gtgaaattct gcaactcaat tgcaggcgtg caggcgatac 600 gggcagactt gagtactaca ggggagactg gaattcctgg tgtagcggtg aaatgcgcag 660 atatcaggag gaacaccggt ggcgaaggcg ggtctctggg tagtaactga cgctgaggag 720 cgaaagcgtg gggagcgaac aggattagat accctggtag tccacgccgt aaacggtggg 780 tactaggtgt ggggctcatt tcacgagttc cgtgccgtag ctaacgcatt aagtaccccg 840 cctggggagt acggccgcaa ggctaaaact caaaggaatt gacgggggcc cgcacaagcg 900 gcggagcatg tggattaatt cgatgcaacg cgaagaacct tacctgggtt tgacatacac 960 cagaaagccg tagagatacg gccccccttg tggttggtgt acaggtggtg catggctgtc 1020 gtcagctcgt gtcgtgagat gttgggttaa gtcccgcaac gagcgcaacc cttgtcctgt 1080 attgccagcg ggttatgccg gggacttgca ggagactgcc ggggtcaact cggaggaagg 1140 tggggatgac gtcaagtcat catgcccctt atgtccaggg cttcacacat gctacaatgg 1200 cgcgtacaga gggctgcgag accgtgaggt ggagcgaatc ccttaaagcg cgtctcagtt 1260 cggattgggg tctgcaactc gaccccatga agtcggagtc gctagtaatc gcagatcagc 1320 aacgctgcgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacgt catgaaagtc 1380 ggtaacaccc gaagccggtg gcctaaccc 1409

Claims (9)

A strain Gordonia cholesterolivorans HTS-S2 (KCTC13663BP) with the ability to degrade tetramethylammonium hydroxide (TMAH). Including a strain of claim 1, Lai shinny Bacillus spare Li coarse HTS-S3 (Lysinibacillus sphaericus HTS- S3) (KCTC13664BP) and a stacking notes Pomona's malto pilriah HTS-S1 (Stenotrophomonas maltophilia HTS- S1) (KCTC13662BP) strains &Lt; / RTI &gt; The method according to claim 2, Acinetobacter genus Strain IBN-H6 (Acinetobacter sp IBN- H6;. KCTC 10809BP), Acinetobacter genus IBN-H7 (Acinetobacter sp IBN- H7;. KCTC 0836BP), Clube My process Delphi four cis IBN-H1 (Kluvermyces delphenisis IBN- H1; KCTC 0834BP), Bacillus cereus IBN-H4 (Bacillus cereus IBN- H4;. KCTC 0835BP), Mycobacterium species SMIC-1 (Mycobacterium sp SMIC- 1 KCTC 10947 BP) and a bacterium of methyl Solarium X Toku Enschede SMIC-1 ( Methylobacterium extorquens SMIC-1 KCTC 10946 BP). &Lt; / RTI &gt; A porous carrier comprising the TMAH decomposition composition of claim 2. The porous carrier according to claim 4, wherein the carrier is a hexahedral polyurethane foam. A method of microbial water treatment comprising contacting wastewater comprising TMAH with the strain of claim 1, the composition of claim 2 or 3, or the porous carrier of claim 4 or 5. According to claim 6, wherein said carrier is in Acinetobacter Strain IBN-H6 (Acinetobacter sp IBN- H6;. KCTC 10809BP), Acinetobacter genus IBN-H7 (Acinetobacter sp IBN- H7;. KCTC 0836BP), Clube My process Delphi four cis IBN-H1 (Kluvermyces delphenisis IBN- H1; KCTC 0834BP), Bacillus cereus IBN-H4 (Bacillus cereus IBN- H4;. KCTC 0835BP), Mycobacterium species SMIC-1 (Mycobacterium sp SMIC- 1 KCTC 10947 BP) and a bacterium of methyl Solarium X Toku Enschede SMIC-1 ( Methylobacterium extorquens SMIC-1 KCTC 10946 BP). &Lt; / RTI &gt; 7. The microbial water treatment method according to claim 6, wherein the microbial water treatment is carried out under aerobic conditions at 15 캜 to 42 캜. The microbial water treatment method according to claim 8, wherein the TMAH of the influent wastewater is 4 to 7 g / L, the pH of the reaction tank is adjusted to a range of 5-8, and the operation is continued under the condition of a residence time of 24-120 hours .

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