KR101942516B1 - Manufacture method of marsh snail extract - Google Patents

Abstract

More particularly, the present invention relates to a method for producing an extract of a polygonum tumefaciens, for effectively extracting active ingredients of polyglycerol using an acid and an enzyme.
A method for preparing an extract of a dragonfly according to the present invention comprises the steps of: preparing a dragonfly; A second step of preparing a mixture by adding water and an enzyme to the prepared sugar cake; A third step of firstly extracting the mixture by first heating; A fourth step of secondary heating the extract prepared by the first extraction; A fifth step of adding an acid to the extract which has been secondarily heated, followed by a second reaction by reacting; A sixth step of neutralizing the second extract; A seventh step of filtering the neutralized extract; And concentrating the filtered extract under reduced pressure.

Description

Manufacture method of marsh snail extract

More particularly, the present invention relates to a method for producing an extract of a polygonum tumefaciens, for effectively extracting active ingredients of polyglycerol using an acid and an enzyme.

It is a kind of shellfish belonging to the reptile family. It lives in a clean river of 1 ~ 2 water, which is deep and watery, and in a gap between rocks such as a lake.

It is known that it is effective for protection of eyesight, restoration of liver function and resolution of hangover, and it is also beneficial for anemia because of iron content.

These foods are consumed in a variety of dishes in order to consume such foods as health food, and they also consume extracts, concentrates, and seaweeds.

Korean Patent Registration No. 0558649, which utilizes the merits and features of such a tea syrup, is characterized in that it comprises the steps of adding water to the tea syrup and then heating it at 100 to 120 캜 for 24 hours to extract the tea syrup extract, And the mixture is subjected to filtration and filtration for 3 to 5 hours at a temperature of 100 ° C to form a concentrated liquid. The extract is then pulverized to separate the bark and flesh of the bark. A step of washing, filtering, dewatering and sterilizing the extracted flesh to remove moisture, and then keeping it at -2 ° C for 5 hours for freezing to remove the odor of the intestine, thereby forming pure fleshy meat; A method for producing a food composition having water and meat quality, and Korean Patent Laid-Open Publication No. 2004-0091418 discloses a method for preparing a soybean milk concentrate The surface of the obtained extrudate is washed and the extruded extrudate is allowed to stand in a water of more than one grade to remove impurities and then the coarse extrudate is pulverized to prepare a pulverized pulverized product, 1 to 1: 3 to 1: 1, and heating the mixture for 30 to 50 hours in a hot water bath.

However, the known methods for extracting tea leaves have not been sufficiently extracted, such as fragrance components and amino acids contained in tea leaves, resulting in a decrease in efficiency. Accordingly, there is a need for a more effective method for producing tea leaves.

Korean Patent Publication No. 2004-0091418

Disclosure of Invention Technical Problem [8] Accordingly, the present invention has been made to solve the above problems, and an object of the present invention is to provide a method for manufacturing a polyglycerol extract solution in which an effective component such as a fragrance component and an amino acid is more effectively extracted, .

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not restrictive of the invention as set forth in the accompanying drawings. It will be possible.

A method for preparing an extract of a dragonfly according to the present invention comprises the steps of: preparing a dragonfly; A second step of preparing a mixture by adding water and an enzyme to the prepared sugar cake; A third step of firstly extracting the mixture by first heating; A fourth step of secondary heating the extract prepared by the first extraction; A fifth step of adding an acid to the extract which has been secondarily heated, followed by a second reaction by reacting; A sixth step of neutralizing the second extract; A seventh step of filtering the neutralized extract; And concentrating the filtered extract under reduced pressure.

According to the solution of the above-mentioned problems, the method of the present invention for extracting the polyphenols of the present invention can more efficiently extract the polyphenols from the polyphenols by proteolytic action of the enzymes and acids during the polyphenol extract.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a flow chart showing a method for producing a tea leaf extract of the present invention. FIG.

The above and other objects, features and advantages of the present invention will be more apparent from the following detailed description taken in conjunction with the accompanying drawings, in which: FIG. BRIEF DESCRIPTION OF THE DRAWINGS The advantages and features of the present invention and the manner of achieving them will become apparent with reference to the embodiments described in detail below with reference to the accompanying drawings.

Hereinafter, the method for producing the tea extract of the present invention will be described in detail with reference to the drawings.

1 is a flow chart showing a method for producing a tea extract of the present invention.

First, in the first step S10, a crossing is prepared. Specifically, the pulp is selected and washed to prepare the pulp.

Next, in a second step (S20), water and enzyme are added to the prepared agar slurry to prepare a mixture. Specifically, water and an enzyme extract used as a solvent are added to and mixed with the prepared skim milk powder to prepare a mixture.

The enzyme extract is preferably an alkalase (acalase 2.4 L). The enzyme extract acts to efficiently extract amino acids and fragrance components through a proteolytic reaction.

Preferably, the mixing ratio of the polyglycerol, water, and enzyme is 5 parts by weight of water and 0.5 part by weight of the enzyme, based on 1 part by weight of the polysaccharide.

If the amount of water is less than 5 parts by weight based on 1 part by weight of water, the amount of water serving as a solvent may be insufficient and extraction may not be smooth. If the amount of water is more than 5 parts by weight, The work may be prolonged in the process of concentration to be performed.

If the amount of the enzyme is less than 0.5 part by weight based on 1 part by weight of the polysaccharide, the effect on the proteolytic activity of the enzyme may not be sufficiently expressed. If the amount is more than 0.5 part by weight, have.

Next, in the third step (S30), the mixture is firstly heated and subjected to primary extraction. Specifically, the mixture containing the enzyme is first heated with hot water to effect extraction.

It is preferable that the temperature is 60 ° C. If the primary heating temperature is lower than 60 ° C, there is a problem in that the extraction time of the polysaccharide is excessively long. If the primary heating temperature is higher than 60 ° C, the action of the enzyme may be stopped.

The heating time in the primary heating is preferably 3 hours. If the primary heating time is less than 3 hours, the components of the drying agent may not be sufficiently extracted, and if the primary heating time exceeds 3 hours, the critical point for the drying of the drying agent may reach the critical point and the operation time may become excessively long.

Next, in the fourth step (S40), the extract is subjected to secondary heating. Specifically, the extract prepared by the first heating is secondarily heated with a hot water bath to stop the enzyme reaction, so that the acid reaction to be carried out is enabled.

It is preferred that the temperature is 95 to 100 ° C. If the secondary heating temperature is lower than 95 ° C, the action of the enzyme may not be stopped and the extract may be deteriorated. If it exceeds 100 ° C, the viscosity of the extract may become stronger, and the fragrance and pharmacological components of the extract may be released.

The heating time in the secondary heating is preferably 30 minutes. If the second heating time is less than 30 minutes, the enzyme reaction may not be sufficiently stopped. If the second heating time is more than 30 minutes, the enzyme reaction may stop completely and the efficiency of the operation may be lowered.

Next, in the fifth step (S50), acid is added to the extract which has been secondarily heated, followed by a second extraction. Specifically, acid is added to the secondarily heated extract, and the reaction and extraction are progressed while heating with the hot water.

The acid added for the reaction is hydrochloric acid (HCl), and it is preferable that an acid of about 2N (normal concentration), which is harmless to the human body and capable of sufficient reaction, is added.

The amount of the hydrochloric acid is preferably 5 parts by weight based on 1 part by weight of the first step (S10). If the amount of the hydrochloric acid is less than 5 parts by weight, the protein degradation due to the acid reaction may not be sufficient. If the amount of the hydrochloric acid exceeds 5 parts by weight, the acid is added to the excessive acid, And the like.

The secondary extraction is carried out in a hot water bath, preferably at 95 to 100 ° C. If the temperature is lower than 95 ° C, the extraction operation may not proceed smoothly or the extraction time may become excessively long. If the temperature exceeds 100 ° C, the fragrance and pharmacological components of the polyglycerol extract may fly.

The secondary extraction time is preferably 3 hours. If the secondary extraction time is less than 3 hours, sufficient extraction through the proteolytic effect of the acid may not be performed. If the secondary extraction time exceeds 3 hours, the critical point for the extraction may be reached and the efficiency of the operation may be lowered.

Next, in the sixth step (S60), the secondary extract is neutralized. Specifically, the extract extracted secondarily through the acid reaction is neutralized with a base.

The base to be neutralized is sodium hydroxide (NaOH), and the pH of the extract is neutralized to 7.0 by using sodium hydroxide at a concentration of 33% which is harmless to the human body and capable of being neutralized.

Next, in the seventh step (S70), the neutralized extract is filtered. Specifically, the sludge is separated from the neutralized extract and filtered under reduced pressure.

It is preferable to use the above-mentioned chips of 80mesh. If the mesh of the network is smaller than 80 mesh, the filtration time becomes longer and the efficiency of the operation is lowered. If the mesh is larger than 80 mesh, the filtration may not be performed properly.

Next, in the eighth step (S80), the filtered extract is concentrated under reduced pressure. Specifically, the filtered extract is concentrated under reduced pressure to complete the preparation of the extract.

Hereinafter, the experimental results will be described in detail in order to set the optimum conditions for the extraction time and the effect of the preparation of the extract using the enzyme and the acid.

A. Verification of the effect of producing extract using enzyme

[Comparative Example 1]

In Comparative Example 1, the tea extract was prepared by hot water extraction for 3 hours.

[Example 1]

Example 1 is a tea extract prepared by the first step of extraction using an enzyme in the method of preparing the extract of the present invention for 1 hour (secondary extraction - no acid extraction) followed by sludge removal and concentration.

[Example 2]

Example 2 is a tea extract prepared by conducting the first extraction using an enzyme in the method of the present invention for 3 hours (second extraction - no extraction) followed by sludge removal and concentration.

[Example 3]

In Example 3, the extract of the present invention was prepared by conducting the first extraction using an enzyme for 5 hours (second extraction - no extraction) and then removing and concentrating the sludge.

division Sugar content Sludge weight per 400g
(80 mesh) Sludge dry weight
(80 mesh) Comparative Example 1 0.46 316.7 85.55 Example 1 1.51 267.89 68.97 Example 2 2.07 227.83 56.28 Example 3 2.17 223.3 48.37

As shown in Table 1, the sugar content of Examples 1 to 3 was higher than that of Comparative Example 1, and the weight of the sludge was also significantly decreased. Therefore, it can be seen that the extraction method using the enzyme is more effective than the general hot water extraction.

In Examples 1 to 3, the changes in sugar content and sludge weight according to the heating time were similar to those in Examples 2 to 3. Therefore, the heating time of the first extract was 3 hours The most preferable one can be found.

N. Verification of the effect of extracts using acid

[Comparative Example 1]

In Comparative Example 1, the tea extract was prepared by hot water extraction for 3 hours.

[Example 4]

In Example 4, the extract of the present invention was prepared by extracting and concentrating the sludge after the secondary extraction with acid was conducted for 1 hour (primary extraction - no enzyme extraction).

[Example 5]

In Example 5, the extract of the present invention was prepared by extracting and concentrating the sludge after the secondary extraction using acid was conducted for 3 hours (primary extraction - no enzyme extraction).

[Example 6]

In Example 6, the extract of the present invention was prepared by extracting and concentrating sludge after the secondary extraction using acid was conducted for 5 hours (primary extraction - no enzyme extraction).

division Sugar content Sludge weight per 400g
(80 mesh) Sludge dry weight
(80 mesh) Comparative Example 1 0.46 316.7 85.55 Example 4 5.77 254.41 47.15 Example 5 6.72 249.44 32.64 Example 6 6.96 246.06 30.85

As shown in Table 2, the sugar content of Examples 4 to 6 was higher than that of Comparative Example 1, and the weight of the sludge was also significantly decreased. Therefore, it can be seen that the acid extraction method is more effective than the general hot water extraction.

C. Effect of enzyme-acid extraction

[Comparative Example 1]

In Comparative Example 1, the tea extract was prepared by hot water extraction for 3 hours.

[Example 7]

In Example 7, the extract of the present invention was prepared by conducting the first extraction using the enzyme for 3 hours, the second extraction using the acid for 1 hour, removing the sludge, and concentrating the extracted extract.

[Example 8]

In Example 8, the extract of the present invention was prepared by conducting the first extraction using the enzyme for 3 hours, the second extraction using the acid for 3 hours, and the sludge removal and concentration.

[Example 9]

In Example 9, the extract of the present invention was prepared by conducting the first extraction using the enzyme for 3 hours, the second extraction using the acid for 5 hours, and the sludge removal and concentration.

division Sugar content Sludge weight per 400g
(80 mesh) Sludge dry weight
(80 mesh) Comparative Example 1 0.46 316.7 85.55 Example 7 7.01 125.40 24.44 Example 8 7.24 90.20 10.91 Example 9 7.26 88.75 10.12

As shown in Table 3, the sugar content of Examples 7 to 9 was higher than that of Comparative Example 1, and the weight of the sludge was also significantly decreased. Therefore, it can be understood that the extraction method using the enzyme-acid is more effective than the general hot water extraction.

In addition, the sugar content of Examples 7 to 9 was higher than those of Examples 1 to 6 shown in Tables 1 and 2, and the weight of sludge was also decreased. Therefore, it can be understood that the extraction method in which the enzyme-acid is sequentially applied is more effective than the extraction method in which the enzyme and the acid are used singly.

Since Examples 8 to 9 show similar changes in sugar content and sludge weight according to the heating time in Examples 7 to 9, the heating time of the secondary extracts was changed from 3 hours to 8 hours in consideration of efficiency and productivity The most preferable one can be found.

The results of the free amino acid analysis of Comparative Example 1 and Examples 7 to 9 in the above-mentioned experiment are shown below in order to confirm the effect of increasing the amino acid content during the preparation of the extract of the present invention.

D. Free amino acid analysis

division Comparative Example 1 Example 7 Example 8 Example 9 O-Phosopho-L-serine 69.00 27.33 11.67 14.00 Taurine - - - 0.67 O-Phosphoethanolamine - - 0.33 1.00 Urea 8.67 2.33 1.00 2.00 L-Aspartic acid 25.00 108.67 343.00 620.00 Hydroxy-L-proline - - - - L-Theronine 15.33 21.67 37.00 66.33 L-Serine 12.00 36.33 67.00 118.33 L-Asparagine 0.67 10.33 9.33 6.00 L-glutamic acid 88.00 171.33 281.00 474.00 Theanine - 11.33 19.67 8.00 D, L- [alpha] -amino adipic acid - 16.67 27.67 25.00 L-proline - - - - Glycine 25.00 35.00 113.33 212.33 L-Alanine 85.00 39.67 91.33 168.67 L-Citrulline - - - - L-α-Amino-n-butylic acid 10.67 7.67 12.67 20.67 L-Valine 13.67 11.00 9.00 20.67 L-Cystine 1.00 36.33 36.33 30.00 L-Methionine 9.67 32.67 42.33 69.00 L-Isoleucine 6.33 3.33 6.33 5.33 L-Leucine 14.67 93.00 111.33 173.33 L-Tyrosine 27.67 26.67 35.33 67.67 L-Phenylalanine 14.33 21.33 32.00 55.33 b-Alanine 1.00 10.67 13.33 18.00 D, L-β-Aminoisobutylic acid - 91.33 92.67 118.67 4-Amino-n-butyric acid 6.00 3.00 7.00 11.00 L-Histidine 9.00 10.00 13.67 25.67 1-Methyl-L-histidine - 1.67 3.33 5.00 3-Methyl-L-histidine - 1.33 2.00 4.00 L-Tryptophan - - - - L-Carnosine 14.00 25.00 23.33 35.00 L-Ornithine-monohydrochloride 72.67 8.67 12.33 17.67 L-Lysine 97.33 21.67 42.67 86.00 Ammonium Chloride 8.00 54.33 41.67 41.67 L-Argnine 154.00 37.67 57.33 115.67 Total 788.67 978.00 1597.00 2636.67

As shown in Table 4, the total content of the free amino acids of Examples 7 to 9 was higher than that of Comparative Example 1, and the content of free amino acids such as L-Methionine, L-Histidine, L-Asparagine and Taurine It can be seen that the content is measured to be high.

Therefore, when the extraction is carried out using the above-mentioned enzyme-acid, it is possible to produce a tea extract of higher quality.

As described above, it is to be understood that the technical structure of the present invention can be embodied in other specific forms without departing from the spirit and essential characteristics of the present invention.

Therefore, it should be understood that the above-described embodiments are to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than the foregoing description, All changes or modifications that come within the scope of the equivalent concept are to be construed as being included within the scope of the present invention.

S10. The first step to prepare the dragon
S20. A second step of preparing a mixture by adding water and an enzyme to the prepared poly
S30. And a third step of firstly extracting the mixture by first heating
S40. The fourth step of secondary heating the extract prepared by the first extraction
S50. A fifth step in which an acid is added to the secondarily extracted extract, followed by a second extraction
S60. The sixth step of neutralizing the second extract
S70. The seventh step of filtering the neutralized extract
S80. Step 8 to concentrate the filtrate under reduced pressure

Claims (1)

A first step of preparing the web;
A second step of preparing a mixture by adding water and an enzyme to the prepared sugar cake;
A third step of firstly extracting the mixture by first heating;
A fourth step of secondarily heating the extract prepared by the first extraction with hot water;
A fifth step of adding an acid to the extract which has been secondarily heated, then reacting and extracting it with a hot water;
A sixth step of neutralizing the second-extracted extract to pH 7.0;
A seventh step of filtering the neutralized extract with a mesh of 80 mesh;
And concentrating the filtered extract at a reduced pressure,
In the third to fourth steps, the primary heating temperature is extracted at 60 ° C for 3 hours, the secondary heating temperature is heated at 95 to 100 ° C for 30 minutes,
In the fifth step,
At a temperature of 95 to 100 DEG C for 3 hours,
In the second step, the addition amount of water and enzyme is 5 parts by weight of water and 0.5 part by weight of enzyme,
In the fifth step, the acid is 2N (normal concentration) hydrochloric acid (HCl), 5 parts by weight with respect to 1 part by weight of the first step,
The enzyme is an alkaline,
Wherein the neutralization is carried out in an aqueous solution of sodium hydroxide (NaOH) at a concentration of 33% in the sixth step

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