KR101931050B1 - Analysis method for feature and quality control of jeju dendropanax morbifera - Google Patents

Abstract

The present invention relates to an analysis method for feature and quality control of a Jeju Dendropanax morbiferus extract, and more particularly, to an analysis method for feature and quality control of a Dendropanax morbiferus extract, in which the content of kaempherol was analyzed to establish the method of differentiating raw materials of the Dendropanax morbiferus extract, and the analysis method using the same is developed to be able to manage the quality of a product using the Dendropanax morbiferus extract.

Description

METHOD FOR FEATURE AND QUALITY CONTROL OF JEJU DENDROPANAX MORBIFERA.

The present invention relates to a method for analyzing the characteristics and quality of the extract of Hokutogi, and more particularly, to a method for analyzing the characteristics and quality of Hokutogi extract using the content of the indicator component.

Dendropanax morbifera Lev. Is a subtropical evergreen arboreous tree of Araliaceae. It is distributed in southern coastal area and Jeju Island in Korea. Jeju is widely distributed in Mt. Halla, but it is mainly grown in 400 ~ 600m above sea level, mainly in the Donnaeko Valley and Suokak Valley, which are now difficult to access by ordinary people. Yellow-eyed tree is a species that does not support fallen leaves in the winter. If you bruise the bark, yellow resinous liquid comes out and it is called 黄 漆. Huangchil has been used as a rare paint for the emperor's armor, helmet, and other metal ornaments since the Three Kingdoms period. It has been used in the Goryeo period, which was written in the Goryeo period, the Guilin variety in China, And it is said that it was a special product of Baekje which was before it, and it remains in the passage of the book department of the Tang history book. It is said that it is effective for burning removal, eye quality, burn treatment, leprosy, harmlessness, and it is used recently for tea and food.

Recently, the beneficial effects such as excellent anticancer and antimicrobial effects of Hwangchujang have been revealed, and industrialization using it as a raw material has been actively carried out, and farmers who grow it are also increasing. The harvesting of Hwangchil trees occurs around May before the flowering period and around November after the fruits have been harvested. It is usually between November and February, although there is a difference of about one month in each region. In the summer of August, immature fruit can be mixed and it is not suitable for the harvest time. Therefore, the harvesting or harvesting of yellowtail is hardly achieved in August at the farmers.

Currently, the industrially produced Hwangchu-wood products are mainly produced by using extracts of leaves and branches of Hwangchujang. Mature leaves are used as the leaves in the raw materials of the Hwangchu-gil, and leaves are used as the branches. It is often used as a raw material when a mixture of 3: 7 ratio of leaf and branches of Hwangchil is widely used. However, due to high price and shortage of raw materials of Hwacheolchu, And there are also cases where the leaves are produced using only the raw materials. Thus, the standard for managing the quality of the extract of Hokkaido tree used in the products has been ambiguous.

In the case of products using natural products, the entire plant may be used, but since only a part of the plant is selectively used, it is not easy to discriminate between the products. In addition, there is a tendency that the content is varied depending on various factors such as the cultivation time of the natural product, the environment, and the variability. Accordingly, a method for distinguishing the quality of a natural product by measuring the content of the natural product using the marker and comparing the measured content with the standard is disclosed. The indicator component is chemically defined in natural products. It is used for quantitative or qualitative analysis for the purpose of controlling the quality of products using natural products. It is used as an active marker or an analytical marker .

KR 101490711 B1

The present invention aims to provide a method for quality control of the extract of Hokkaido japonica extract by setting a criterion for use in assessing the quality of Hokkaido japonica extract by confirming the change of the surface component content of the Hokkaido japonica extract according to various conditions . More particularly, the present invention aims to provide a method for quality control of a product using the extract of Huangchu tree by establishing a method for discriminating raw materials of Huchilchu extract by analyzing an indicator component utilizing the calibration curve method and developing an analysis method using the same.

According to an aspect of the present invention, there is provided a method for preparing a crude oil extract, comprising the steps of: preparing a calibration curve of camphor as an index component for evaluating the quality of the extract; A second step of performing HPLC (High Performance Liquid Chromatography) on the extract of Hwangcholgulchiae obtained by differentiating at least one condition from the extraction or extraction of raw materials and obtaining a peak area from the chromatogram; A third step of deriving a content of camphor in the Hwigae mackerel extract using the regression equation of the first step and the peak area of the second step; A fourth step of setting a quality control standard using the camphor content derived in the third step; A fifth step of performing HPLC on the target Hwanyeongnam extract and determining the peak area from the chromatogram; A sixth step of deriving a content of camphor in the to-be-tested Tochigi leaf extract using the regression equation of the first step and the peak area of the fifth step; And a seventh step of comparing the quality control standard of the fourth step and the camphor content of the sixth step to evaluate the quality of the to-be-tested Tochigi leaf extract, .

In addition, the first step may include the steps of: preparing a standard solution of camphor; Performing HPLC according to the standard solution and obtaining a peak area from the chromatogram; And generating a calibration curve using the peak area and the concentration as standard variables.

Further, the above regression equation can be expressed by the following equation (1).

[Equation 1]

(Where y is the chromatogram peak area and x is the camphor concentration).

In addition, it is preferable that the content of Camphor in the Hwanyeolmum extract in the seventh step is 0.19 to 0.98 mg / mL.

In addition, simultaneous analysis can be performed by using quercetin as the indicator component.

According to the analysis method according to one aspect of the present invention, an indicator method is provided for checking the difference in the content of the indicator components of the Hwigaechuang Extract according to various conditions to trace back the ratio of the ingredients of the Hwigae . Through this, it is possible to derive the ratio of the harvested portion of the raw materials of Horticulture used in the Horticultural Extract or the mixed use of the other raw materials. Or the quality control standard of the extract itself can be set.

FIG. 1 is a flow chart of an analysis method for quality control of an extract of Euphorbiaceae according to an embodiment of the present invention,
FIG. 2 shows the classification criteria of the raw material of Hwangchulchu according to the harvesting site,
FIG. 3 is an HPLC chromatogram of the indicator components quercetin, camphorol and isorhamnetin,
FIG. 4 is a calibration curve and a regression equation of the indicator components quercetin, camphorol and isorhamnetin.

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings. It will be apparent to those skilled in the art that these embodiments are illustrative of the present invention and are not intended to limit the scope of the appended claims and that various changes and modifications may be made to the embodiments within the scope and spirit of the invention , And it is natural that such variations and modifications fall within the scope of the appended claims. In the following description of the present invention, detailed description of known functions and configurations incorporated herein will be omitted when it may make the subject matter of the present invention rather unclear.

FIG. 1 is a flowchart illustrating an analysis method for quality control of the extract of U. pergola according to an embodiment of the present invention.

Referring to FIG. 1, the analysis method for quality control of the extract of U. perilla according to an embodiment of the present invention comprises the steps of: preparing a calibration curve of an indicator component to derive a regression equation; Establishing criteria for quality control of Horticultural extract; And a step of deriving the surface component content of the Tochigiokus extract to be evaluated and evaluating the quality thereof.

First, a step (S1) of deriving a regression equation by selecting an indicator component and creating a calibration curve is performed. Indicator components are low in variability due to differences in harvesting sites, sampling periods, receipts, or regional factors of Hwangchilgae raw materials, and there are abundant characteristics data including toxicity, It is desirable that the components be selected. As a result of screening various components to find out the index component which can be applied to the raw material, manufacturing process and the final product of Hwangchu - wood, it is distinguished from other components in the leaves of Hwangchu - Camerol (kaempferol, C ₁₅ H ₁₀ O ₆ ) was selected as an indicator component. If necessary, quercetin (C ₁₅ H ₁₀ O ₇ ) or isorhamnetin (C ₁₆ H ₁₂ O ₇ ) can be further used as an indicator component. The presence of quercetin is confirmed in many other food materials, and its specificity is confirmed in the extract of Hwangchujang, and it can be used together with camphorol as an indicator component to discriminate the mixing ratio of each part of Hwangchuek. Isolametin is not detected in the extract of Hwangchilan tree, and it can be used together with camphorol as another index component which is not detected in the Hwangchil tree extract. In detail, by using quercetin as another indicator component, it is possible to improve the discrimination or estimation reliability of the blossoming percentage of yellowtail tree of the sample to be evaluated, and isoramnetin is used as another indicator component, It is possible to predict whether other materials are mixed or not. Quercetin or isorhamnetin is an index component that can be analyzed simultaneously with camphorol. By using quercetin or isorhamnetin in addition to camphorol, it can improve the quality control reliability and provide various information.

The index component used in the present invention is quantified using a calibration method. In order to measure the concentration or content of a specific substance in a sample, the standard solution solution having a known accurate concentration is measured by an analyzer, and the value is stored. The signal value of the sample to be analyzed and the signal value of the standard solution It is a method to convert the concentration by using. The standard substances camperol, quercetin and isorhamnetin were each dissolved in a solvent for high performance liquid chromatography (HPLC) and prepared as standard solutions at various concentrations. From the chromatograms using the absorbance values calculated by HPLC, A peak area of each solution is determined, and a calibration curve is prepared by using the peak area and the concentration of the standard solution as parameters to obtain a regression linear equation (hereinafter referred to as a regression equation) for each reference material.

Next, a step S2 is performed to set a criterion for utilizing in the quality control of the extract. In this step, step (S21) of performing HPLC on the extract obtained from various varieties of Hokkaido chrysanthemum; (S22) deriving the content of camphor in the Hokkaido extract; And setting a quality control standard using the same (S23).

In order to establish the quality control standard for the extract of Horticultura, it is desirable to acquire information which is a standard for various conditions and to make it into a database. Therefore, it is better to obtain the Horticultural Extract by differentiating at least one condition of the raw material . In detail, it is preferable to obtain the extract of Hokkaido chinensis by at least one condition selected from the group consisting of the harvesting site, the harvesting time, the harvesting time and the extraction solvent of the Hokutogi source. Then, HPLC is performed for each extract obtained. It is preferred that the Hwigaechu extract is pretreated and then subjected to HPLC. The pretreatment can be carried out by adding a solution containing sulfuric acid and methanol and carrying out an acid hydrolysis treatment at 80 to 95 ° C. The peak area is obtained from the chromatogram of each extract obtained by HPLC. The index substance concentration in the extract is calculated for each extract using the regression formula of the index component obtained in the previous step (S1) and the peak area for each of the extracts, When the drainage and sample weight are corrected again, the content of the indicator component is derived, and the quality control standard is set by using the derived indicator content of each extract. For example, the indicator material content of each extract is databaseed, and the content of each condition is set as a reference point, or a preferable index material content is calculated in consideration of sampling time and sampling site of the commonly used extract, .

Next, a step (S3) of deriving the surface component content of the Tochigi extract and evaluating the quality thereof is carried out. In this step, a step (S31) of carrying out HPLC on an extract of Tochigi koreana is conducted; (S32) deriving the content of camphor in the tofu extract; And evaluating the quality thereof (S33).

The peak area was obtained from the chromatogram of the Hwangchu extract of the subject and quantitatively analyzed by using the obtained peak area and the regression equation. The quality control standard set in the previous step S2 and the indicator substance content of the to-be-tested Tochigi can be evaluated to evaluate the quality of the Tochigi extract. For example, if there is a significant difference in the content of indicator substances in the extract to be evaluated based on the index substance content database of various extracts of Hokkaido tree extracts, the incorporation of other raw materials may be inferred. Alternatively, by checking the difference in the indicator material content by condition, it is possible to trace the mixed ratio of the raw material material of the subject to be evaluated in reverse by referring to the mixing ratio of the harvesting time and the harvesting site.

Hereinafter, the specific structures and effects of the present invention will be described in more detail with reference to the following examples.

Example

≪ Preparation of an extract of Wuchulia chinensis>

In order to establish the quality control standard, various extracts of Hwangchu - In detail, the extract powder was prepared by dividing by the harvesting site, sampling period, receipt date and extracting solvent of the Hwangchil tree raw material. Hwangchilchu was collected from Jeju City and Seogwipo City, and 5, 8, 10 and 15 year olds were collected and the harvesting site was divided into leaves, branches and fruits. As shown in Fig. 2, the leaves and branches of the harvesting site were again divided into a young green part and a green mature part. Finally, the harvesting part was divided into a larval leaf, a mature leaf, a leaf, a raw egg and a fruit . The harvesting time was different from May, August and November, and raw materials were prepared. In the case of Leaf or Leaf Leaf, it is rarely used industrially as a raw material. In case of fruit, it is not allowed to be edible under the Food Act, . Each raw material was finely divided into 2 cm and dried by hot air drying at 50 ° C for 15 hours, and then extracted with an extraction solvent as a raw material. The extracts of 30% (v / v) alcohol and 60% (v / v) water were the most widely used in the extraction of hot pepper, Extracts were prepared by extracting different solvents. The alcohol was diluted with distilled water and used. The optimum conditions were selected by selecting the optimum conditions for each condition. The final extraction conditions were: 3 hours at 85 ℃ for hot water extraction, 75 ℃ for 30% (v / v) 4 hours, and 70% (v / v) for 4 hours. After extraction, it was filtered, concentrated and lyophilized to obtain powdery Hwangchuek tree extract for each raw material. Table 1 shows the extracts of Hwangchujang which were prepared by different conditions.

No. By region When to collect Year Extraction solvent One Fruit September 10 years Heat number 2 Fruit November 10 years Heat number 3 Seed Leaf In May 8 years Heat number 4 Seed Leaf In May 10 years Heat number 5 Seed Leaf In May 15 years Heat number 6 Seed Leaf November 8 years Heat number 7 Seed Leaf November 10 years Heat number 8 Seed Leaf November 15 years Heat number 9 Mature leaf In May 8 years Heat number 10 Mature leaf In May 8 years 30% alcohol 11 Mature leaf In May 10 years Heat number 12 Mature leaf In May 10 years 30% alcohol 13 Mature leaf In May 10 years 60% alcohol 14 Mature leaf In May 15 years Heat number 15 Mature leaf In May 15 years 30% alcohol 16 Mature leaf August 10 years Heat number 17 Mature leaf August 10 years 30% alcohol 18 Mature leaf November 8 years Heat number 19 Mature leaf November 8 years 30% alcohol 20 Mature leaf November 10 years Heat number 21 Mature leaf November 10 years 30% alcohol 22 Mature leaf November 10 years 60% alcohol 23 Mature leaf November 15 years Heat number 24 Mature leaf November 15 years 30% alcohol 25 Possession In May 8 years Heat number 26 Possession In May 10 years Heat number 27 Possession In May 15 years Heat number 28 Possession November 8 years Heat number 29 Possession November 10 years Heat number 30 Possession November 15 years Heat number 31 One way In May 8 years Heat number 32 One way In May 8 years 30% alcohol 33 One way In May 10 years Heat number 34 One way In May 10 years 30% alcohol 35 One way In May 10 years 60% alcohol 36 One way In May 15 years Heat number 37 One way In May 15 years 30% alcohol 38 One way August 10 years Heat number 39 One way August 10 years 30% alcohol 40 One way November 8 years Heat number 41 One way November 8 years 30% alcohol 42 One way November 10 years Heat number 43 One way November 10 years 30% alcohol 44 One way November 10 years 60% alcohol 45 One way November 15 years Heat number 46 One way November 15 years 30% alcohol

<Preparation of calibration curve of surface indices>

Examination and screening were carried out on the extracts of Hwangchilchu tree, and the camphorol was identified as an index component of HPLC peaks in the leaves of Hwachunil. In addition, quercetin was identified as an indicator component of the extract of Hwangchulchae. Among the flavonoids, isoramnetin was not detected in the extracts of Hwangchilchu tree. As a comparative reference, Isolamnetin, which is a component of flavonoids and not detected in Hwangchulchil, was selected as another index component.

In order to quantify the surface components, camptol, quercetin and isorhamnetin, which are standard substances, were dissolved in MeOH for HPLC and 1 mg / mL stock solution was prepared. In detail, quercetin was administered at concentrations of 5 μg / mL, 10 μg / mL, 25 μg / mL, 50 μg / mL and 100 μg / mL, camelol and isorhamnetin at 1 μg / mL, μg / mL, 25 μg / mL and 50 μg / mL, respectively. 20 μL of each standard solution was taken as a sample and the area was determined from the chromatogram obtained by HPLC. A calibration curve was prepared using these areas and the concentration of the standard solution as parameters, and a regression equation was obtained. The HPLC chromatogram for standard substances is shown in Fig. 3, and the calibration curve is shown in Fig. The regression equation of camphor is represented by the following equation (1), the regression equation of quercetin is represented by the following equation (2), and the regression equation of isorhamnetine is expressed by the following equation (3).

(In the above Equations 1 to 3, y is the chromatogram peak area of the sample subjected to HPLC, and x is the concentration of the indicator component of the sample subjected to HPLC.)

<Quantitative analysis of surface elements>

Quantitative analysis of the indicator components was carried out on the extracts of P. japonicus in Table 1.

For the preparation of the test solution to be subjected to HPLC, 0.1 g of the powdery extract was precisely taken, 3 ml of 50% (v / v) MeOH containing H ₂ SO ₄ was added and acid hydrolysis was carried out at 90 ° C for 30 minutes. The hydrolyzed solution was placed in a 25-mL constant volume flask, diluted with 50% (v / v) MeOH, filtered through a 0.45 μm membrane filter, and used as the test solution. 20 μL of each test solution was subjected to HPLC, and the peak area was obtained from the chromatogram obtained. The content of each indicator component was obtained from the regression equation. In detail, the peak area of the test solution as the unknown sample was substituted into the y variable of the above Equations 1 to 3, and the concentration was calculated by deriving the concentration of the sample by the test solution as the x variable. HPLC was performed using Empower3 software program (Waters Corporation, Milford, Mass., USA) for data acquisition and processing, using 1525 binary pump, column oven and 2998 photodiode array detector And the peak area was obtained. HPLC conditions are shown in Table 2 below. Table 3 shows the results of the quantitative analysis of the surface components of the extracts of Hwangchujang (Table 1) using HPLC chromatogram and the dilution drainage and sample weights were corrected again to obtain the index material contents of the Hwachunilchu extracts according to the conditions. In Table 3, ND means Not Detected.

Mobile phase
(mobile phase) Time (min) 0.1% TFA in H ₂ O 0.1% TFA in ACN 2 80 20 25 55 45 30 0 100 31 80 20 column
(column) Phenomenex C18 column (4.6 x 250 mm, 5 [mu] m) Detector
(detector) Waters 2998 UV detector (370 nm) Flow rate
(flow rate) 1 ml / min Injection capacity
(injection volume) 20 μL

No. By region When to collect Year Extraction solvent Surface composition (mg / g) Quercetin Camphor roll Isorhamnetine One Fruit September 10 years Heat number N.D. N.D. N.D. 2 Fruit November 10 years Heat number N.D. N.D. N.D. 3 Seed Leaf In May 8 years Heat number 16.32 0.25 N.D. 4 Seed Leaf In May 10 years Heat number 10.20 0.28 N.D. 5 Seed Leaf In May 15 years Heat number 17.20 0.98 N.D. 6 Seed Leaf November 8 years Heat number 4.23 0.24 N.D. 7 Seed Leaf November 10 years Heat number 9.72 0.50 N.D. 8 Seed Leaf November 15 years Heat number 1.37 0.22 N.D. 9 Mature leaf In May 8 years Heat number 5.37 0.32 N.D. 10 Mature leaf In May 8 years 30% alcohol 7.20 0.35 N.D. 11 Mature leaf In May 10 years Heat number 5.86 0.30 N.D. 12 Mature leaf In May 10 years 30% alcohol 18.06 0.50 N.D. 13 Mature leaf In May 10 years 60% alcohol 20.15 0.52 N.D. 14 Mature leaf In May 15 years Heat number 19.51 0.40 N.D. 15 Mature leaf In May 15 years 30% alcohol 19.75 0.57 N.D. 16 Mature leaf August 10 years Heat number 10.51 0.58 N.D. 17 Mature leaf August 10 years 30% alcohol 12.61 0.63 N.D. 18 Mature leaf November 8 years Heat number 8.68 0.47 N.D. 19 Mature leaf November 8 years 30% alcohol 13.03 0.63 N.D. 20 Mature leaf November 10 years Heat number 0.82 0.19 N.D. 21 Mature leaf November 10 years 30% alcohol 6.59 0.20 N.D. 22 Mature leaf November 10 years 60% alcohol 9.98 0.54 N.D. 23 Mature leaf November 15 years Heat number 2.24 0.28 N.D. 24 Mature leaf November 15 years 30% alcohol 8.03 0.50 N.D. 25 Possession In May 8 years Heat number 0.41 N.D. N.D. 26 Possession In May 10 years Heat number 0.30 N.D. N.D. 27 Possession In May 15 years Heat number 0.33 N.D. N.D. 28 Possession November 8 years Heat number 0.78 N.D. N.D. 29 Possession November 10 years Heat number 0.44 N.D. N.D. 30 Possession November 15 years Heat number 0.49 N.D. N.D. 31 One way In May 8 years Heat number 0.42 N.D. N.D. 32 One way In May 8 years 30% alcohol 0.33 N.D. N.D. 33 One way In May 10 years Heat number 0.57 N.D. N.D. 34 One way In May 10 years 30% alcohol 0.52 N.D. N.D. 35 One way In May 10 years 60% alcohol 0.94 N.D. N.D. 36 One way In May 15 years Heat number 0.55 N.D. N.D. 37 One way In May 15 years 30% alcohol 0.94 N.D. N.D. 38 One way August 10 years Heat number 0.35 N.D. N.D. 39 One way August 10 years 30% alcohol 0.28 N.D. N.D. 40 One way November 8 years Heat number 0.71 N.D. N.D. 41 One way November 8 years 30% alcohol 1.13 N.D. N.D. 42 One way November 10 years Heat number 0.94 N.D. N.D. 43 One way November 10 years 30% alcohol 1.03 N.D. N.D. 44 One way November 10 years 60% alcohol 1.05 N.D. N.D. 45 One way November 15 years Heat number 0.56 N.D. N.D. 46 One way November 15 years 30% alcohol 0.83 N.D. N.D.

As shown in Table 3 above, camphorol was specifically detected only in the extracts obtained from the extracts of the leaves of the perennials. In the mature leaves, the extracts of 10% And 30% (v / v), respectively. The highest content was obtained at 0.98 mg / mL of hot water extracts from the leaves of 15 - year - old Hwangchu - Camphorol can be used as an indicator component to provide a criterion for determining whether or not the leaves are used and the ratio of the use thereof.

Quercetin showed significant differences in the contents of the extracts according to the order of pickling, sampling period and sampling sites. Quercetin was found to be detected at higher contents than leaves and leaves. In the case of 60% (v / v) extracts of 10 - year - old mature leaves of 10 - year old harvested from May, Respectively. Quercetin can be detected as an indicator material for the quality control of common whitewood products by showing that quercetin is detected in all the extracts using the raw material of Hwangchu wood except for the fruit. Therefore, the simultaneous analysis with camphor can be used to improve the reliability of discrimination analysis of the mixed ratio of the raw material of Horticulture.

Isolametin was not detected in all the extracts of Hwangchujang tree, and it could be used as an indicator to determine the addition of Hwangchilgae and other raw materials.

With reference to the quantitative results in Table 3, a camphor peak is distinctly detected in an extract using a leaf of a Japanese whitish wood as a raw material, and a low-cost extract of a Japanese whitneywood using an eggplant can be distinguished. In addition, it can be predicted that quercetin and camphorol peak are characteristically detected and isorhamnetine is not detected in a conventional extract using a mixture of branches and leaves and used as an extraction raw material. In addition, the dilution factor and the sample weight are corrected to the quantitative value of the indicator component, and the index substance content of the extract of U. peruviana can be obtained. Using the quantitative results shown in Table 3, the change of the content of the indicator component by the condition can be confirmed, and it can be used as a quality control standard to derive the use ratio of the leaves and branches among the natural material raw materials used in the manufacture of the extract. . For example, when the analysis method according to the present invention is applied to the Hokutake japonica extract to be evaluated, it is possible to deduce the incorporation of other harvesting sites or other materials when the content of the indicator component is remarkably low or high. , And can be used to determine the grade of the product using the Hokutogi extract and to manage the quality. In addition, when two or more indicators are combined without using a single indicator component, the reliability of analysis can be further improved. For example, since quercetin exists in many other food materials, it is possible to predict the incorporation of other materials when the content of quercetin in the sample to be analyzed is much higher than the content range derived from the results of Table 3 above, More reliable results can be obtained depending on the detection of the net.

In the case of extracts from raw pomegranate leaves, it is judged that camphorol is more preferably detected based on the value of 0.19 mg / g, which is the minimum value of camphor in Table 3, as a reference point. With reference to the contents in Table 3 above, camphorol was detected up to 0.63 mg / g based on mature leaves and up to 0.98 mg / g based on sardine leaves. The content of quercetin in the extracts of raw pomegranate leaves was 0.82 to 20.15 mg / g. When the matured leaves and the raw leaves, which are the most commonly used conventional whitish wood raw materials, are considered on the basis of the extract of Hokutogi, which is mixed at a weight ratio of 3: 7, the camphor is classified into each minimum value and maximum value by applying the ratio to be detected in less than 0.057 to 0.189mg / g value obtained, in the case of kwosetin is minimum is 100: 0.82 = 30: x ₁ and 100: 0.28 = 70: after computing the x ₂ x ₁ and x ₂ and the maximum value is calculated as the sum of x ₃ and x ₄ after calculating 100: 20.15 = 30: x ₃ and 100: 1.13 = 70: x ₄ and is within a quercetin content of 0.442 to 6.836 mg / g It can be judged that it is desirable to be detected.

According to the analysis method of the present invention, it is possible to judge the quality of the extract of Huchulchuang by differentiating or estimating the mixing ratio of the Huchulchuang extract or the mixing ratio with the other substances. As a result, it can be used as a quality control method for controlling the quality of the woody products using the extract.

Claims (5)

delete delete delete delete The concentrations of camelol and cholesterol were determined as 1 μg / mL, 5 μg / mL, 10 μg / mL, 25 μg / mL and 50 μg / mL, respectively. A standard solution prepared by dissolving in methanol at a concentration of 5 μg / mL, 10 μg / mL, 25 μg / mL, 50 μg / mL and 100 μg / mL to prepare a calibration curve to derive a regression equation;
In order to establish the quality control standard for the extract of Hokutogi, extracts were prepared by extracting 30% (v / v) or 60% (v / v) A second step of performing an acid hydrolysis treatment at 80 to 95 占 폚 by adding a solution containing sulfuric acid and methanol to the extract of Hwangcholgwa extract to perform high performance liquid chromatography (HPLC) and obtaining a peak area from the chromatogram;
A third step of deriving the content of camphor and quercetin in the extract of Hwigae et al. Using the regression equation of the first step and the peak area of the second step, and databaseing the index material content of the Hwacheonchu extract according to the condition;
A fourth step of setting a quality control standard using the camphor and quercetin content derived in the third step;
A fifth step of performing HPLC on the target Hwanyeongnam extract and determining the peak area from the chromatogram;
A sixth step of deriving the camphor content and the quercetin content in the to-be-tested Tochigi extract using the regression equation of the first step and the peak area of the fifth step; And
And a seventh step of comparing the quality control standard of the fourth step and the content of camphor and quercetin of the sixth step to evaluate the quality of the to-be-tested Tochigi leaf extract. A method for quality control for extracting Hwacheonchu extract, which is able to derive the ratio of used parts or the mixture of different raw materials,
Characterized in that isomalin is further used as the indicator component and simultaneously analyzed.

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