KR101919895B1 - Composition for controlling pest comprising citral - Google Patents

Abstract

The present invention provides a composition for pest control including citral and a manufacturing method thereof. The composition including citral and the manufacturing method thereof of the present invention can be usefully used as pest control techniques in a pest control field. The composition for pest control comprises: 5 to 10 parts by weight of citral; 1 to 10 parts by weight of PEG-60 hydrogenated castor oil; 0.1 to 2 parts by weight of glycerin; 0.01 to 1 parts by weight of EDTA2Na; 0.01 to 1 parts by weight of citric acid; 0.01 to 1 parts by weight of sodium citrate; and ethanol 30 to 70 parts by weight.

Description

TECHNICAL FIELD The present invention relates to a composition for controlling pest including citral,

TECHNICAL FIELD The present invention relates to a pest control composition, and more particularly, to a pest control composition comprising citral.

Vesicular arthropods are not only animals such as poultry but also animals that cause human stress and cause stress such as disturbance of sleep and weight loss by mediating disease or direct blood sucking. It is used for mosquito, tick (mites, mites) Flies, flies, flies, etc.), etc., lice flies, bedbugs, mites.

Among these arthropods, mosquitoes mediate infectious diseases such as malaria, yellow fever, dengue fever, Japanese encephalitis, West Nile virus, Zicca virus, and Chikungunya. Spider-rod arthropods, which are mainly parasitic to animals such as small mite mites and fleas, are important mediators mediating Lyme disease, spot fever, rickettsia, severe febrile platelets syndrome, and Tsutsugamushi disease.

Repellents are known to be the most appropriate means to block disease mediated by blood-borne arthropods. DEET (N, N-diethyl-m-toluamide) has been recognized as a repellent against various insects including mosquitoes. However, it is known that DEET has various adverse effects such as unpleasant odor, plastic or rubber damage, central nervous system depression, dermatitis induction, and mammalian DNA damage.

Korean Patent Publication No. 2009-0130037 discloses a hydrazide compound and a harmful arthropod-controlling agent containing the same, and Korean Patent No. 1012547 discloses a 1,2,4-thiadiazole compound and Korean Patent Laid-Open Publication No. 2016-0079851 discloses substituted benzamide for arthropod control.

In recent years, studies on plant-based essential oils have been conducted as eco-friendly blood-circulating arthropod repellents. However, in general, plant essential oils have lower efficacy against arthropods such as mosquitoes and ticks than organic synthetic repellents, There is a problem of using a large amount.

Korean Patent Publication No. 10-2018-0001760 (2015.01.05.) Korean Patent Laid-Open No. 10-2009-0100321 (September 23, 2009)

The inventors of the present invention have been studying pest control agents that have a good anti-alopecia effect on veterinary arthropods and have no adverse effects on human beings. However, the inventors of the present invention have found that compositions containing citral exhibit excellent anti- And can be used for controlling purposes.

Accordingly, it is an object of the present invention to provide a composition for controlling insect pests comprising citral and a method for producing the same.

According to one aspect of the present invention, there is provided a pest controlling composition comprising citral.

In one embodiment, the citral may be included in an amount of 1-10% by weight, and the composition may further comprise PEG-60 hydrogenated castor oil, glycerin, EDTA-2Na, sodium citrate and citric acid.

In one embodiment, the composition comprises 1 to 10 parts by weight of citral, 1 to 10 parts by weight of PEG-60 hydrogencarbonated castor oil, 0.1 to 2 parts by weight of glycerin, 0.01 to 1 part by weight of EDTA-2Na, 0.01 to 1 part by weight of citric acid, 1 part by weight of sodium citrate, 0.01 - 1 part by weight of sodium citrate and 30 - 70 parts by weight of ethanol.

In one embodiment said pest is selected from the group consisting of a blood-sucking arthropod, preferably Leptotrombidium scutellae , Haemaphysalis longicornis , Dermatophagoides pteronyssinus and Dermatophagoides farinae ) May be used.

In one embodiment, the composition can be in the form of a liquid or a spray.

In one embodiment, when the citral is included in an amount of 1 - 10% by weight, the salicidal effect of the dust mite when applied to a house dust mite including longitudinal dermatophagoides pteronyssinus and dermatophagoides farinae , And the body color may be changed.

(A) adding EDTA-2Na, citric acid and sodium citrate to water and dissolving it completely, adding glycerin and stirring to obtain a liquid phase; (b) mixing the ethanol with citral and PEG-60 hydrogenated castor oil to obtain an oil phase; And (c) solubilizing the oil phase obtained in step (b) in the liquid phase obtained in step (a) with stirring and stirring.

It has been found by the present invention that a composition comprising citral exhibits excellent alleviating efficacy against blood-borne arthropods, in particular ticks.

Accordingly, the composition comprising citral of the present invention and the method for its production can be usefully used as pest control techniques in the field of pest control.

Fig. 1 is a photograph of (A) a captive site of Jeonnam crustacean, (B) a sherman trap installation, and (C) a pulling method.
Fig. 2 is a photograph of (A) captor, (B) parasitic mite (orange), (C) mite on the water.
Fig. 3 is a photograph of (A) residue contact test of fleas, (B) test petri dish (diameter of 5 cm), and (C) residue contact test of true ticks.
FIG. 4 is a result of observing changes in body color over time after spraying 5.0% citral into house dust mite.

The present invention provides a pest control composition comprising citral.

And citral (citral) are referred to as 3,7-dimethyl-2,6-octadienyl day (3,7-dimethyl-2,6-octadienal ) or Le monal (lemonal), molecular formula C ₁₀ H ₁₆ O, A mixture of terpenoids, which is a mixture of double bond isomers. The E -isomer is called geranial or citral A, and the Z -isomer is neral or citral B.

In one embodiment of the present invention, the citral may be included in an amount of 1 to 10% by weight, preferably 5 to 10% by weight, and the composition comprises PEG-60 Hydrogenated Castor Oil ), Glycerin, EDTA-2Na, sodium citrate, and citric acid. At this time, PEG-60 hydrogenated castor oil is used as a solubilizing agent, glycerin is used as a humectant, EDTA-2Na is used as a stabilizer, and sodium citrate and citric acid are used as pH adjusters.

In one embodiment, the composition comprises 1 to 10 parts by weight of citral, 1 to 10 parts by weight of PEG-60 hydrogencarbonated castor oil, 0.1 to 2 parts by weight of glycerin, 0.01 to 1 part by weight of EDTA-2Na, 0.01 to 1 part by weight of citric acid, 1 part by weight of sodium citrate, 0.01 - 1 part by weight of sodium citrate and 30 - 70 parts by weight of ethanol.

In one embodiment, when the citral is included in an amount of 1 - 10% by weight, the salicidal effect of the dust mite when applied to a house dust mite including longitudinal dermatophagoides pteronyssinus and dermatophagoides farinae , And the body color may be changed.

(A) dissolving EDTA-2Na, citric acid and sodium citrate in water, adding glycerin and stirring to obtain a liquid phase; (b) mixing the ethanol with citral and PEG-60 hydrogenated castor oil to obtain an oil phase; And (c) solubilizing the oil phase obtained in step (b) in the liquid phase obtained in step (a) while stirring and agitating the oil phase.

The composition of the present invention prepared as described above can be used as a liquid formulation in a light yellow liquid, and can also be used as a spraying agent.

In one embodiment of the invention, the insect pests can be blood-sucking arthropods, such as Leptotrombidium scutellae , Haemaphysalis longicornis , Dermatophagoides pteronyssinus , And dermatophagoides farinae , but the present invention is not limited thereto.

The efficacy of the composition according to the present invention on the mites was 92.2% at 120 minutes (2 hours), and 97.8% at 150 minutes (2 hours 30 minutes) . As a result of probit analysis, the LT ₅₀ was 62.9 (58.4 - 67.4) min, the LT ₉₅ was 134.4 (121.8 - 152.1) min, and the treatment amount of the citral major component per unit area was 0.25 mg / cm < ² >, and 2,500 mg / m < ^{2 &} gt ;.

In addition, the effective mortality rate for small sphagnum mites was 87.8% at 300 minutes (5 hours) and 97.8% at 360 minutes (6 hours). As a result of the Probit analysis, the LT ₅₀ was 170.2 (158.3 - 181.2) minutes, the LT ₉₅ was 360.4 (326.4 - 411.0) minutes, the treatment amount of the citral main component per unit area was 0.25 mg / cm ² , ² .

As described above, it has been confirmed that the composition for insect control comprising citral of the present invention exhibits an excellent salvage efficacy against the blood-sucking arthropod pests.

Hereinafter, the present invention will be described in more detail through examples and test examples. However, the following examples and test examples are provided for illustrating the present invention, and the scope of the present invention is not limited thereto.

<Examples>

1. Preparation of citral 5% preparation

The citral preparations were prepared according to the following procedure with the compositions shown in Table 1 below.

① EDTA-2Na, citric acid (citric acid) and sodium citrate (sodium citrate) were added to purified water and stirred for 15 minutes.

② After completely dissolved, glycerin was added and stirred for 15 minutes.

③ Polyethylene glycol 60 castor oil (PEG-60 Hydrogenated Castor oil), which is a citric and a solubilizing agent, was mixed with ethanol and stirred for 20 minutes. Polyethylene glycol 60 Castor oil was used at 50 ° C because it was in a solid state at room temperature.

④ When ① and ③ were added and solubilized while stirring, it became soluble in cloudy state and transparent.

⑤ After filtering, it was stored in a container. The properties of the obtained formulation were a pale yellow liquid, pH 5.6, specific gravity 0.94.

Whole unit Of 100 g Purpose of blending Raw material name standard Unit (g) CAS No. Detailed configuration chief ingredient Citral Expression 5 5392-40-5 Available (Tues) Polyethylene glycol 60 castor oil KQC 1-10 61788-85-0 Moisturizer glycerin KQC 0.1-2 56-81-5 Stabilizer EDTA-2Na KQC 0.01-1 6381-92-6 pH adjusting agent Sodium citrate KQC 0.01-1 6132-04-3 pH adjusting agent Citric acid KQC 0.01-1 77-92-9 solvent ethanol KQC 30-70 64-17-5 solvent Purified water KQC Balance 7732-18-5

2. Manufacture of citral 10% preparation

A 10% citral preparation was prepared in the same manner as in the above item 1 except that 10 g of citral was used.

<Test Example>

The research was commissioned by the Institute of Sanitary Pest Control, Eulji University and tested as follows.

1. Purpose of Testing

We would like to test the saliva efficacy against hairy ticks and mites of citral 5% preparation commissioned by National Science Cycle Co., Ltd.

2. Test method

end. Test animal

1) Leptotrombidium scutellare ) larva

2) Small sphyx mites ( Haemaphysalis longicornis )

I. Collection place

1) Bullwing mite larvae: On November 6th - 14th, 2017, weedworms were caught in the vicinity of Sowong-gil and Naju-si in Ogye-myeon, Geosung-gun, Chonnam Province.

2) Small Sphinx mites: On November 8-11, 2017, the mosquitoes were collected from Myeongsan (Sol-Oum) in Donggu-dong, Seogwipo-si, Jeju Special Self-Governing Province, and near Hoesu-dong.

All. Collection method [see FIG. 1]

1) Sweet white mite: On the previous afternoon, a sherman trap containing bait food was placed in the grass around the paddy field , and the parasitic mite was collected by collecting the trapped Apodemus agrarius the next day.

2) Small Sophora japonica: A small sphagnum mite was collected by pulling on a grass.

la. Obtain test animals

1) Hairy tick larva [see FIG. 2]

① Peanut butter and 2: 1 oatmeal mixed as a bait for the mankind was used.

② Approximately 2g of manned bait in the shaman trap was installed around 17:00 in a grassy area where the lizard was inhabited.

③ The next day, 08:00 - 09:00, trapped traps were picked up to secure the host of the mite larva. Untrapped traps were moved and repositioned to capture the backbone in succession.

④ The trapped louse moves to the laboratory and shakes off the louse in a thick plastic bag. In the vinyl, the chest of the backbone was suffocated by finger pressure.

⑤ The dead louse was fixed on the cradle by hanging it upside down. Then a bowl containing water was installed to let the larvae of the mite coming off the host fall on the water.

⑥ The inverted moths were allowed to stand for about 24 hours so that the mites fell off sufficiently.

⑦ Twenty-four hours later, the mite larvae that fell on the water were collected and stored in a 20 mL vial.

2) Small sphyx mite tick

① Cheju Island has been selected as a gathering area because there are a lot of small sphagnum mites especially in wild animals and grazing areas.

② Small Sphinx mites climb to the end of the pool and move to the passing host animal.

③ A white cloth with a length of 1 m and a length of 1 m is fixed on one side of the same length rod, and both ends of the fixed rod are connected by a line so that they can be easily dragged from the grass.

④ After pulling for about 1-2 minutes with a white cloth made on the grass top, turn the cloth over and check that a small soppy mite is attached to the cloth and collect it in a bottle with tweezers.

⑤ Several small sphagnum mites were collected and used in this test in the same way.

hemp. Test agent

The medicines used in this test were supplied by National Science and Technology, Inc. and used for the test. The composition and presentation methods of the test agents are shown in Table 2 below.

product name Principal component and content Usage Remarks Undefined Citral 5% Hairy mites and small sphygmotic ticks Spray How to present a test client Spray liquid (0.25 mL / 50 cm ² ) at 50 mL / m ² on mite and small sphagnum mite habitat and parasitic site.

bar. Test Methods

1) Residual contact test method (continuous contact test method) [see FIG. 3]

The filter paper was cut into a size of 5 cm in diameter after being dripped with 0.25 mL (based on 50 mL / m ² ) of the citral 5% preparation on the filter paper cut into a size of 10 cm × 5 cm in width and the solvent was evaporated in the room. The cut filter paper was attached to a 5 cm diameter Petri dish bottom surface, and 10 ticks were put into a nail art brush, and then checked continuously to observe the lethal individual and calculate the mortality rate.

2) Control group

50% ethanol was added dropwise to 50 cm ² filter paper in an amount of 0.25 mL (50 mL / m ² ), dried and used.

3) Hair mite salivation effect test

① An active bursodactyle larvae on a Petri dish (5 cm in diameter) filter paper prepared by the residue contact test method was confirmed by a dissecting microscope and 10 mice were put into the filter paper. Ten mice were administered to the mice in triplicate.

② The control group was also given the same method as that of the barnyard mite and tested until the same time when mite was killed in the test group.

③ The Petri dish lid was not closed, and when the larvae of the mite leaves the treated filter paper, the mite is moved to the center of the filter paper with the brush, so that the contact with the treated surface continues.

④ The test was carried out in a room maintained at 25 - 26 ℃. No humidity was supplied to the test container, and the test table was tested with the humidifier turned on. The activity and mortality of the larvae were examined under a dissecting microscope, and lethal time and mortality were calculated in comparison with the control group.

⑤ The above test was repeated 3 times in total.

4) Small Sophora tick tick effect test

(1) The same method as that of the hair mite test was carried out. Ten mites of the small sphagnum mite were visually examined and put into the test for a total of 3 times.

The control group was also given the same amount of small sphagnum mites and tested until the same time when the mites were killed in the test group.

③ The Petri dish lid was not closed, and when the small sphagnum mite larvae were moved out of the filter paper treated with the drug, the mite was moved to the center of the filter paper with the brush, so that the drug contacted the treated surface.

④ The test was carried out in a room maintained at 25 - 26 ℃. No humidity was supplied to the test container, and the test table was tested with the humidifier turned on. During the test, the degree of lethality of small sphagnum mites was closely observed under a dissecting microscope, and lethal time and mortality were calculated in comparison with the control group.

⑤ The above test was repeated 3 times in total.

5) Body color change test of house dust mite

① House dust mite was inoculated with a thin pin made of 90 x 15 mm Petri dish and 90 mm filter paper.

② Control (control, methanol) and citral 5.0% Two kinds of liquids were sprayed into the filter paper five times with their 100 mL plastic containers.

③ After marking a certain area with a pencil in a circle, the change of body color was observed with an optical microscope (x40 times) and pictures were taken with a camera.

④ The above test was conducted three times in total.

3. Test results

end. Mite Salvi  effect

Table 3 below shows the effect of the biting mite on the mite according to the elapsed time after exposure to the medicament.

Test group Average lethal rate (%) with elapsed time (min) ± SD 30 60 90 120 150 Control group 0 0 0 0 0 Citral 5% 5.6 ± 5.1 48.9 ± 5.1 72.2 ± 6.9 92.2 ± 6.9 98.9 ± 1.9

I. Small Sophie Salvi  effect

Table 4 below shows the anti-inflammatory effect of small sphagnum mites on the time elapsed after exposure to the drug.

Test group Average lethal rate (%) with elapsed time (min) ± SD 120 180 240 300 360 Control group 0 0 0 0 0 Citral 5% 25.6 ± 5.1 50.0 ± 3.3 76.7 ± 3.4 87.8 ± 1.9 97.8 ± 3.9

All. Body color change of house dust mite

FIG. 4 shows the results of observing changes in body color after spraying 5.0% citral into house dust mite.

As shown in FIG. 4, it was observed that the mite color changed from white (no color, transparent color) to yellow after 1 hour, 3 hours, 5 hours, and 24 hours after spraying 5.0% citral.

4. Summary of results

end. Mite Salvia effect

The effective mortality rate of the citral 5% citrus larvae was 92.2% at 120 minutes (2 hours) and 97.8% at 150 minutes (2 hours 30 minutes).

The results of probit analysis showed that the LT ₅₀ was 62.9 (58.4 - 67.4) min and the LT ₉₅ was 134.4 (121.8 - 152.1) minutes for the mite larvae of the citral 5% formulation.

Therefore, the treatment amount of the citral principal component per unit area is 0.25 mg / cm ² and is 2,500 mg / m ² .

I. Small Sophie Salvia effect

The effective mortality rate for the small sphagnum mites of the citral 5% formulation by the residue contact test method was 87.8% at the elapsed time of 300 minutes (5 hours) and 97.8% at the elapsed time of 360 minutes (6 hours).

The probit analysis showed that the LT ₅₀ for small sphagnum mites of the citral 5% formulation was 170.2 (158.3 - 181.2) min and the LT ₉₅ was 360.4 (326.4 - 411.0) min.

Therefore, the treatment amount of the citral principal component per unit area is 0.25 mg / cm ² and is 2,500 mg / m ² .

All. Body color change of house dust mite

Citral formulations for house dust mite the vertical pattern house dust mite (Dermatophagoides pteronyssinus) and the application (spray method) appears and when live biryeok a large bridge house dust mite (Dermatophagoides farinae), was confirmed to be gradually mite body color after 1 time variation.

5. Conclusion

The saliva effect of citral is due to the presence of several tailed goose mites ( Rhipicephalus microplus ) has an LC ₅₀ of 7.0 mg / mL (7,000 ppm) and a large legged dust mite ( Dermatophagoides farinae ), LC _5O is 1.13 μg / cm ² . It has been found that citral is a slightly aromatic compound that increases mortality and lethal time faster than open space when exposed to citral compounds in the closed space with mites and small sphagnum mites. Therefore, the citral compounds appear to exhibit inactivity both in contact and in fumigation, and are expected to show synergistic effects in controlling true tick control. In particular, the citral ingredient is listed as a food additive and can be safely used in comparison with other pest control compounds. Therefore, it is possible to use the corticosteroids (Tsutsugamusi kiss medium), the small sphygmotic ticks (SFTS viral mediator ) And house dust mites.

Claims (9)

5 to 10 parts by weight of citral, 1 to 10 parts by weight of PEG-60 hydrogenated castor oil, 0.1 to 2 parts by weight of glycerin, 0.01 to 1 part by weight of EDTA-2Na, 0.01 to 1 part by weight of citric acid, 0.01 to 1 part by weight of sodium citrate, , small Sophie true tick (Haemaphysalis longicornis), vertical pattern house dust mite (Dermatophagoides pteronyssinus) and for large bridge house dust mite (Dermatophagoides farinae) pests at least one member selected from the group consisting of a control containing 70 parts by weight to 1 part by weight ethanol 30 Composition. delete delete delete delete delete The insect control composition according to claim 1, wherein the composition is a liquid or a spray. The method of claim 1, wherein the pest to the pest control during application of the composition for the vertical pattern house dust mite (Dermatophagoides pteronyssinus) and large bridge house dust mite (Dermatophagoides farinae) salbi efficacy is characterized in that occurrence of phenomenon that the body color of the tick body changes (2). (a) 0.01 to 1 part by weight of EDTA-2Na, 0.01 to 1 part by weight of citric acid and 0.01 to 1 part by weight of sodium citrate are dissolved in water and 0.1 to 2 parts by weight of glycerin is added thereto and stirred to obtain a liquid phase;
(b) mixing 30 to 70 parts by weight of ethanol with 5 to 10 parts by weight of citral and 1 to 10 parts by weight of PEG-60 hydrogenated castor oil to obtain an oil phase; And
(c) adding the oil phase obtained in the step (b) to the liquid phase obtained in the step (a) and solubilizing it with stirring
Wherein the composition is at least one selected from the group consisting of Haemaphysalis longicornis , Dermatophagoides pteronyssinus and Dermatophagoides farinae .

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